Rmu_sc0017357.1_g000003

Vacuolar protein sorting-associated protein 62

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017357.1
OG0010762
Physical Location & Seq
Reverse (-)
15689 .. 17998
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017357.1_g000003.1.cds

Sequence Viewer

Length: 1716 bp
atgattgggtgtgagtgttggtgctggagcagtgaatatgaggagtacctatatgatcagtctgagccaaagccatactctttgccttcgcctcttccaaaatggcctcaaggccaaggttttgcgactggaagaatatgccttggagaaattgaagttgtgcaaatcacaaagtttgagactgtctggagctgcaatgtgttgcatggaaaatccaaaggtgtcgcattttatagacctgctgggattcccgatgacttcttttgcctcggtcactactgccaacccaatgaccagccgttaagaggttttgtacttgctgctcgtgacacggttgctaccaggacggatttgaaagatggttttgtccaagacttggtacaaaagttgccagctttaataaagccccttaactactccttagtctggaatgcagattcgaccagtgggtgtggttatatttggatgccaaacccacctgtgggttataaggccatgggttttgtggtcactgacgatagtgaggagccaagcctcgacgttgttcgatgtgtacgagaagatcttactgagacttgtgaaccgtgtgatcggatacttgcagtggattcgaagttttcagcaaaccaatttcaggtttggagtacaagaccttgcgaacgaggtatgttctgtagaggtgtttcagttggcacattcttttgttctagtacctacttggattctgacgaagaactagaagttgcgtgcttgaagaatattggttcatccctacacgcaatgccaaatctacagcaggttcataccctcattgagcactatgggcctactgtatacttccatcctgatgaggcttatttgccatcgtcagtacaatggtttttcaaaaatggggcacttttgtacggtgattcctctgaaacaggtgaatcaattgatcacagaggctctaatttgcctaggggaggggaaaatgagcatgattattggatagatttgccaaatgatgatgatgctagacatcatatcaaaggcggagacatggagagtgcgaagctatatgttcatgtaaaaccagcattaggaggaactttcaccgatgtggccatgtggatattctgtcccttcaatggaccggccaccattaaagttggtgtagtgagtattgcaatgaacaagataggacaacatgttggtgattgggagcactacactctccgagtgagcaacttcaccggagaactttggcaggtgtatttctcagaacacagtggtggtagatgggtggatgcttttgacttggagttcattgaagggaataagtcaattgtatattcttcaaaacatggtcatagtagctaccctcattcaggaacctatcttcaggggtcttcaaagcttggaataggagtaaagaacgaagttgctcgaagcaagatgttcattgattcgagcaccaaatatgagattgttgccgccgagtaccttggtgatggtgttattgaagaaccgtgttggttgcagtatatgagagattggggtccaaccattgtgtatgattcgcgatcagaactagataagataattgatcgtcttccattctttgttcgattctctgtggagaatctattcgaattgtttccaaccgaactctacggtgaggaagggccgaccggccccaaggagaaggataattggttgggagatgaaaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps62

Protein Analysis

571

Amino Acids

64.35

Weight (kDa)

4.88

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010762)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04350 AT3G04350
fragaria_vesca FvH4_6g01580
malus_domestica MD00G1149300.v1.1 MD04G1232300.v1.1 MD07G1000700.v1.1
prunus_persica Prupe.2G000800_v2.0.a1 Prupe.6G351900_v2.0.a1
pyrus_communis pycom04g20570 pycom12g23000
rosa_chinensis RchiOBHm_Chr3g0449321
rosa_laevigata RLG00000025839
rosa_multiflora Rmu_sc0017357.1_g000003
rosa_roxburghii Rroxscaffold_6G00427330
rosa_rugosa Rorug02G0619700
rosa_samantha Rh3AG017700 Rh3BG017800 Rh3CG016800
rosa_wichuraiana Rw3G001360 Rw3G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AarI CACCTGC 1 cut(s) 1240
Acc36I ACCTGC 3 cut(s) 247, 787, 1240
AccB7I CCANNNNNTGG 1 cut(s) 376
AccI GTMKAC 1 cut(s) 834
AccII CGCG 1 cut(s) 1564
AciI CCGC 2 cut(s) 1035, 1476
AcoI YGGCCR 2 cut(s) 1104, 1137
AcuI CTGAAG 1 cut(s) 1367
AfaI GTAC 9 cut(s) 47, 315, 381, 555, 646, 712, 873, 905, 1484
AflIII ACRYGT 1 cut(s) 1189
AgsI TTSAA 9 cut(s) 155, 355, 754, 886, 1129, 1313, 1341, 1395, 1505
AjnI CCWGG 1 cut(s) 341
AleI CACNNNNGTG 2 cut(s) 1100, 1272
AluBI AGCT 5 cut(s) 192, 395, 1057, 1359, 1399
AluI AGCT 5 cut(s) 192, 395, 1057, 1359, 1399
Alw21I GWGCWC 3 cut(s) 819, 1209, 1457
Alw26I GTCTC 3 cut(s) 173, 566, 1032
AoxI GGCC 8 cut(s) 104, 112, 492, 824, 1104, 1137, 1667, 1675
ApeKI GCWGC 2 cut(s) 192, 320
Asp700I GAANNNNTTC 2 cut(s) 1628, 1638
AspA2I CCTAGG 1 cut(s) 959
AspS9I GGNCC 5 cut(s) 824, 1133, 1541, 1667, 1676
AsuHPI GGTGA 7 cut(s) 920, 938, 1087, 1208, 1225, 1502, 1670
AsuII TTCGAA 2 cut(s) 611, 1632
AvaII GGWCC 2 cut(s) 1133, 1541
AvrII CCTAGG 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 898
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BalI TGGCCA 1 cut(s) 1106
BauI CACGAG 1 cut(s) 324
BbsI GAAGAC 2 cut(s) 1383, 1586
Bbv12I GWGCWC 3 cut(s) 819, 1209, 1457
BbvI GCAGC 2 cut(s) 179, 307
BccI CCATC 5 cut(s) 353, 849, 871, 1275, 1487
BceAI ACGGC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BciT130I CCWGG 1 cut(s) 343
BciVI GTATCC 1 cut(s) 588
BclI TGATCA 2 cut(s) 55, 937
BcoDI GTCTC 3 cut(s) 173, 566, 1032
BfaI CTAG 5 cut(s) 708, 737, 960, 1017, 1574
BfmI CTRYAG 2 cut(s) 673, 791
BfuAI ACCTGC 3 cut(s) 247, 787, 1240
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 1 cut(s) 562
BisI GCNGC 3 cut(s) 193, 321, 1476
BlnI CCTAGG 1 cut(s) 959
BlsI GCNGC 3 cut(s) 194, 322, 1477
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 2 cut(s) 1133, 1541
BmgT120I GGNCC 5 cut(s) 824, 1133, 1541, 1667, 1676
BmiI GGNNCC 4 cut(s) 528, 1375, 1542, 1678
BmrFI CCNGG 1 cut(s) 343
BmsI GCATC 3 cut(s) 456, 1003, 1279
BpiI GAAGAC 2 cut(s) 1383, 1586
BpmI CTGGAG 2 cut(s) 46, 208
Bpu14I TTCGAA 2 cut(s) 611, 1632
BpuEI CTTGAG 1 cut(s) 93
BsaJI CCNNGG 7 cut(s) 115, 142, 268, 495, 959, 1486, 1680
BsaWI WCCGGW 1 cut(s) 1235
Bse118I RCCGGY 2 cut(s) 1135, 1673
Bse1I ACTGG 2 cut(s) 133, 444
Bse3DI GCAATG 3 cut(s) 202, 786, 1176
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 7 cut(s) 115, 142, 268, 495, 959, 1486, 1680
BseGI GGATG 4 cut(s) 471, 767, 841, 1294
BseMI GCAATG 3 cut(s) 202, 786, 1176
BseMII CTCAG 3 cut(s) 54, 561, 1275
BseNI ACTGG 2 cut(s) 133, 444
BseRI GAGGAG 2 cut(s) 56, 539
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 2 cut(s) 179, 307
BseYI CCCAGC 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 1564
Bsh1285I CGRYCG 1 cut(s) 1674
BshFI GGCC 8 cut(s) 106, 114, 494, 826, 1106, 1139, 1669, 1677
BsiEI CGRYCG 1 cut(s) 1674
BsiHKAI GWGCWC 3 cut(s) 819, 1209, 1457
BsiSI CCGG 3 cut(s) 1136, 1236, 1674
BslFI GGGAC 1 cut(s) 1107
BsmAI GTCTC 3 cut(s) 173, 566, 1032
BsmFI GGGAC 1 cut(s) 1107
BsmI GAATGC 1 cut(s) 436
BsnI GGCC 8 cut(s) 106, 114, 494, 826, 1106, 1139, 1669, 1677
Bsp119I TTCGAA 2 cut(s) 611, 1632
Bsp1286I GDGCHC 4 cut(s) 819, 898, 1209, 1457
Bsp143I GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
Bsp19I CCATGG 1 cut(s) 495
Bsp68I TCGCGA 1 cut(s) 1564
BspACI CCGC 2 cut(s) 1035, 1476
BspANI GGCC 8 cut(s) 106, 114, 494, 826, 1106, 1139, 1669, 1677
BspCNI CTCAG 3 cut(s) 55, 562, 1274
BspFNI CGCG 1 cut(s) 1564
BspLI GGNNCC 4 cut(s) 528, 1375, 1542, 1678
BspMI ACCTGC 3 cut(s) 247, 787, 1240
BspT104I TTCGAA 2 cut(s) 611, 1632
BsrDI GCAATG 3 cut(s) 202, 786, 1176
BsrFI RCCGGY 2 cut(s) 1135, 1673
BsrI ACTGG 2 cut(s) 133, 444
BssAI RCCGGY 2 cut(s) 1135, 1673
BssECI CCNNGG 7 cut(s) 115, 142, 268, 495, 959, 1486, 1680
BssMI GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
BssNAI GTATAC 1 cut(s) 835
BssSI CACGAG 1 cut(s) 324
BssT1I CCWWGG 6 cut(s) 115, 142, 495, 959, 1486, 1680
Bst1107I GTATAC 1 cut(s) 835
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 8 cut(s) 184, 334, 585, 832, 908, 1271, 1512, 1658
Bst6I CTCTTC 1 cut(s) 99
BstBI TTCGAA 2 cut(s) 611, 1632
BstC8I GCNNGC 2 cut(s) 393, 748
BstDEI CTNAG 4 cut(s) 63, 421, 570, 1261
BstDSI CCRYGG 1 cut(s) 495
BstENI CCTNNNNNAGG 2 cut(s) 963, 1368
BstF5I GGATG 4 cut(s) 471, 767, 841, 1294
BstFNI CGCG 1 cut(s) 1564
BstKTI GATC 6 cut(s) 58, 565, 592, 940, 1568, 1591
BstMAI GTCTC 3 cut(s) 173, 566, 1032
BstMBI GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
BstMCI CGRYCG 1 cut(s) 1674
BstMWI GCNNNNNNNGC 1 cut(s) 823
BstNI CCWGG 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 1193
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 2 cut(s) 673, 791
BstSLI GKGCMC 1 cut(s) 898
BstUI CGCG 1 cut(s) 1564
BstV1I GCAGC 2 cut(s) 179, 307
BstV2I GAAGAC 2 cut(s) 1383, 1586
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BstZ17I GTATAC 1 cut(s) 835
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 8 cut(s) 106, 114, 494, 826, 1106, 1139, 1669, 1677
BtgI CCRYGG 1 cut(s) 495
BtsCI GGATG 4 cut(s) 471, 767, 841, 1294
BtsI GCAGTG 2 cut(s) 37, 609
BtsIMutI CAGTG 5 cut(s) 37, 451, 510, 609, 1276
BtuMI TCGCGA 1 cut(s) 1564
BveI ACCTGC 3 cut(s) 247, 787, 1240
Cac8I GCNNGC 2 cut(s) 393, 748
Cfr10I RCCGGY 2 cut(s) 1135, 1673
Cfr13I GGNCC 5 cut(s) 824, 1133, 1541, 1667, 1676
Csp6I GTAC 9 cut(s) 46, 314, 380, 554, 645, 711, 872, 904, 1483
CviAII CATG 8 cut(s) 206, 496, 980, 1042, 1067, 1108, 1190, 1346
CviQI GTAC 9 cut(s) 46, 314, 380, 554, 645, 711, 872, 904, 1483
DdeI CTNAG 4 cut(s) 63, 421, 570, 1261
DpnI GATC 6 cut(s) 57, 564, 591, 939, 1567, 1590
DpnII GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
EaeI YGGCCR 2 cut(s) 1104, 1137
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 1050
Eco130I CCWWGG 6 cut(s) 115, 142, 495, 959, 1486, 1680
Eco47I GGWCC 2 cut(s) 1133, 1541
Eco57I CTGAAG 1 cut(s) 1367
EcoNI CCTNNNNNAGG 2 cut(s) 963, 1368
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 6 cut(s) 115, 142, 495, 959, 1486, 1680
ErhI CCWWGG 6 cut(s) 115, 142, 495, 959, 1486, 1680
FaeI CATG 8 cut(s) 209, 499, 983, 1045, 1070, 1111, 1193, 1349
FaqI GGGAC 1 cut(s) 1107
FatI CATG 8 cut(s) 205, 495, 979, 1041, 1066, 1107, 1189, 1345
FbaI TGATCA 2 cut(s) 55, 937
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 3 cut(s) 193, 321, 1476
FokI GGATG 4 cut(s) 478, 754, 828, 1301
Fsp4HI GCNGC 3 cut(s) 193, 321, 1476
FspBI CTAG 5 cut(s) 708, 737, 960, 1017, 1574
GluI GCNGC 3 cut(s) 193, 321, 1476
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 2 cut(s) 46, 208
HaeIII GGCC 8 cut(s) 106, 114, 494, 826, 1106, 1139, 1669, 1677
HapII CCGG 3 cut(s) 1136, 1236, 1674
Hin1II CATG 8 cut(s) 209, 499, 983, 1045, 1070, 1111, 1193, 1349
HindIII AAGCTT 1 cut(s) 1397
HpaII CCGG 3 cut(s) 1136, 1236, 1674
HphI GGTGA 7 cut(s) 920, 938, 1087, 1208, 1225, 1502, 1670
Hpy166II GTNNAC 3 cut(s) 554, 581, 835
Hpy188I TCNGA 7 cut(s) 64, 594, 727, 919, 1220, 1264, 1570
Hpy188III TCNNGA 7 cut(s) 187, 251, 326, 427, 845, 1371, 1563
Hpy8I GTNNAC 3 cut(s) 554, 581, 835
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 5 cut(s) 96, 1135, 1307, 1658, 1681
HpyCH4III ACNGT 8 cut(s) 184, 334, 585, 832, 908, 1271, 1512, 1658
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 7 cut(s) 163, 195, 205, 434, 602, 1169, 1522
HpyF10VI GCNNNNNNNGC 1 cut(s) 823
HpyF3I CTNAG 4 cut(s) 63, 421, 570, 1261
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 8 cut(s) 209, 499, 983, 1045, 1070, 1111, 1193, 1349
Ksp22I TGATCA 2 cut(s) 55, 937
Kzo9I GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
LmnI GCTCC 4 cut(s) 27, 189, 526, 1204
Lsp1109I GCAGC 2 cut(s) 179, 307
LweI GCATC 3 cut(s) 456, 1003, 1279
MaeI CTAG 5 cut(s) 708, 737, 960, 1017, 1574
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 272, 326, 508
MalI GATC 6 cut(s) 57, 564, 591, 939, 1567, 1590
MboI GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
MfeI CAATTG 2 cut(s) 933, 1326
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 4 cut(s) 819, 898, 1209, 1457
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 8 cut(s) 150, 629, 933, 952, 1326, 1584, 1634, 1693
MluNI TGGCCA 1 cut(s) 1106
MmeI TCCRAC 2 cut(s) 1568, 1667
Mox20I TGGCCA 1 cut(s) 1106
MroXI GAANNNNTTC 2 cut(s) 1628, 1638
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 4 cut(s) 302, 398, 411, 1146
MslI CAYNNNNRTG 4 cut(s) 846, 1100, 1194, 1272
Msp20I TGGCCA 1 cut(s) 1106
MspI CCGG 3 cut(s) 1136, 1236, 1674
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 2 cut(s) 933, 1326
Mva1269I GAATGC 1 cut(s) 436
MvaI CCWGG 1 cut(s) 343
MvnI CGCG 1 cut(s) 1564
MwoI GCNNNNNNNGC 1 cut(s) 823
NcoI CCATGG 1 cut(s) 495
NdeII GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
NlaIII CATG 8 cut(s) 209, 499, 983, 1045, 1070, 1111, 1193, 1349
NlaIV GGNNCC 4 cut(s) 528, 1375, 1542, 1678
NmeAIII GCCGAG 1 cut(s) 1504
NmuCI GTSAC 3 cut(s) 272, 326, 508
NruI TCGCGA 1 cut(s) 1564
NspI RCATGY 1 cut(s) 1193
NspV TTCGAA 2 cut(s) 611, 1632
OliI CACNNNNGTG 2 cut(s) 1100, 1272
PaqCI CACCTGC 1 cut(s) 1240
PciI ACATGT 1 cut(s) 1189
PcsI WCGNNNNNNNCGW 1 cut(s) 553
PctI GAATGC 1 cut(s) 436
PdmI GAANNNNTTC 2 cut(s) 1628, 1638
PflMI CCANNNNNTGG 1 cut(s) 376
PkrI GCNGC 3 cut(s) 194, 322, 1477
PscI ACATGT 1 cut(s) 1189
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 4 cut(s) 528, 1375, 1542, 1678
PspPI GGNCC 5 cut(s) 824, 1133, 1541, 1667, 1676
PsuI RGATCY 1 cut(s) 562
RruI TCGCGA 1 cut(s) 1564
RsaI GTAC 9 cut(s) 47, 315, 381, 555, 646, 712, 873, 905, 1484
RsaNI GTAC 9 cut(s) 46, 314, 380, 554, 645, 711, 872, 904, 1483
RseI CAYNNNNRTG 4 cut(s) 846, 1100, 1194, 1272
SaqAI TTAA 4 cut(s) 302, 398, 411, 1146
SatI GCNGC 3 cut(s) 193, 321, 1476
Sau3AI GATC 6 cut(s) 55, 562, 589, 937, 1565, 1588
Sau96I GGNCC 5 cut(s) 824, 1133, 1541, 1667, 1676
ScrFI CCNGG 1 cut(s) 343
SduI GDGCHC 4 cut(s) 819, 898, 1209, 1457
SfaNI GCATC 3 cut(s) 456, 1003, 1279
SfcI CTRYAG 2 cut(s) 673, 791
SfuI TTCGAA 2 cut(s) 611, 1632
SinI GGWCC 2 cut(s) 1133, 1541
SmiMI CAYNNNNRTG 4 cut(s) 846, 1100, 1194, 1272
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 8 cut(s) 150, 629, 933, 952, 1326, 1584, 1634, 1693
SsiI CCGC 2 cut(s) 1035, 1476
SspI AATATT 1 cut(s) 760
SspMI CTAG 5 cut(s) 708, 737, 960, 1017, 1574
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 6 cut(s) 115, 142, 495, 959, 1486, 1680
TaaI ACNGT 8 cut(s) 184, 334, 585, 832, 908, 1271, 1512, 1658
TaiI ACGT 1 cut(s) 543
TaqI TCGA 8 cut(s) 440, 537, 547, 611, 1429, 1451, 1609, 1632
TaqII GACCGA 1 cut(s) 260
TasI AATT 8 cut(s) 150, 629, 933, 952, 1326, 1584, 1634, 1693
TatI WGTACW 3 cut(s) 313, 644, 871
TauI GCSGC 1 cut(s) 1478
Tru1I TTAA 4 cut(s) 302, 398, 411, 1146
Tru9I TTAA 4 cut(s) 302, 398, 411, 1146
TscAI CASTG 5 cut(s) 37, 451, 517, 609, 1276
TseFI GTSAC 3 cut(s) 272, 326, 508
TseI GCWGC 2 cut(s) 192, 320
Tsp45I GTSAC 3 cut(s) 272, 326, 508
TspDTI ATGAA 6 cut(s) 756, 791, 1055, 1187, 1297, 1432
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 5 cut(s) 37, 451, 517, 609, 1276
Van91I CCANNNNNTGG 1 cut(s) 376
VpaK11BI GGWCC 2 cut(s) 1133, 1541
XagI CCTNNNNNAGG 2 cut(s) 963, 1368
XceI RCATGY 1 cut(s) 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XmaJI CCTAGG 1 cut(s) 959
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 2 cut(s) 1628, 1638
XspI CTAG 5 cut(s) 708, 737, 960, 1017, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.