FvH4_6g01630

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006902
Physical Location & Seq
Forward (+)
938385 .. 945010
6626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01630.t5

Sequence Viewer

Length: 1458 bp
ATGTCTCTGGTCACATTCAACACTCATGGATCTGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGACATTGAACTTTTCTTACGATGGTTATCAGCTATTCCCTTCAATGGTGGTGGTTTTAGTCATGCTGCAATTGCAGAAGGGCTGTCTGAAGCCCTAATGATGTTCTCTACCATTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCTGCTAGTGACCCATGTCCCCTTCCCACACCAGTCTATAGACCACAAATGCAAAACGTGGAGCAGAGTGAAATTTCTGATGTGCAAACCGAAAGCTGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCTTTGTTTCTCTGTCAGTCATCTGTCCAAAACAGCTTCCTAAACTCAGAGCTATTTACAATGCGGGAAAGCGCAACCCGCGGGCAGCAGATCCACCAATTGATAATGTGAAAAACCCCCCCTTTCTCGTTCTAATCTCAGAGAATTTTATTGAGGCGCGGGCTACTTTAACTCGTCCAGCTTTGCCGAAAGCTCCTGTTGCATCAGTAACACAGCCACATGAATGTCTTCCCGGTTGCAGTTCGACCGATCCACCTCCGACTTCTAGGCCATCTGGAAATTCTAATCCTGGGATGTCACAACCAATGAGTAACCTCCAAGGTGGTGCTAGTATTGGGCAATCTGTTCCTGGCATAAACCAAAATCACTCAGGATCCCAAATGGTTCAAAATAAACTTGTTTCAGCATGTCCCTCTGGAACTGGTACAATGATTCCTCCTCTTGGAATCTCTCAACAAGCCCAATCTGGGATACAGTCGGTTGGTGCAAACTATAGTTCGGTATTTAATCCGTTGTCCCAACAGACGTCAAGTGCTCTGCAATCTGCAAGATCCAGTTATGTAAAAGTCTGGGAGGGAAATTTATCTTTACCGGGGAGGAGACAAGGACAACCTGTGGTTATTACTAGATTGGAAGGATACAGAAGGGTTTCAGCACGTGAGACGCTTGCAGCAAAATGGCCACCGACATTGCAAATAGTCAAGTTCGCACCTCTGGAACCCCTGATTAACAAGCAGCAGCAATATTCGCAACTGGCCGACATTCTAGTATTTCGGGTAATGGATGGGCAAGAATTTGTTTTGCAGATGCAAGAAAAGAAGCTTTGTGCTGTCATCAAATTGCCGTCTCAAACACTAGTGCTATATCTTTCGCAGAAAGATTGCTACTTGATTGGAATGCTTTTCCCATGGGCTATGGTTGTGTTAAAGCCACAAGTAGTTGGTCAGCAACAAATGCAACAGCAGCAACCACATCTAACAACAGAAGCCTCAGCTTCAACAGATACAGCAACAGCAGCCGAAGCTACAGCAGCAACAAGTGCAACCTGGTCCAGCAACACAGCAACCACAACGGGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

486

Amino Acids

52.32

Weight (kDa)

8.59

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 40 - 214 1.6e-30 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 905
AccII CGCG 2 cut(s) 457, 535
AciI CCGC 4 cut(s) 440, 455, 457, 535
AclWI GGATC 6 cut(s) 37, 461, 620, 744, 757, 921
AcoI YGGCCR 2 cut(s) 1055, 1131
AcsI RAATTY 5 cut(s) 309, 520, 655, 955, 1169
AcuI CTGAAG 1 cut(s) 174
AcvI CACGTG 1 cut(s) 1034
AcyI GRCGYC 1 cut(s) 902
AfaI GTAC 2 cut(s) 45, 802
AfiI CCNNNNNNNGG 4 cut(s) 110, 818, 843, 1449
AgsI TTSAA 7 cut(s) 19, 74, 109, 182, 218, 764, 1374
AhlI ACTAGT 1 cut(s) 1231
AjnI CCWGG 3 cut(s) 664, 724, 1421
AluBI AGCT 9 cut(s) 98, 333, 412, 428, 557, 569, 1198, 1370, 1400
AluI AGCT 9 cut(s) 98, 333, 412, 428, 557, 569, 1198, 1370, 1400
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 5 cut(s) 9, 60, 970, 1031, 1227
AlwI GGATC 6 cut(s) 37, 461, 620, 744, 757, 921
AoxI GGCC 3 cut(s) 644, 1055, 1131
ApeKI GCWGC 9 cut(s) 131, 239, 461, 1046, 1111, 1114, 1339, 1391, 1406
ApoI RAATTY 5 cut(s) 309, 520, 655, 955, 1169
Asp700I GAANNNNTTC 2 cut(s) 603, 1024
AspLEI GCGC 2 cut(s) 450, 535
AspS9I GGNCC 1 cut(s) 1425
AsuC2I CCSGG 2 cut(s) 609, 969
AvaII GGWCC 1 cut(s) 1425
BalI TGGCCA 1 cut(s) 1057
BamHI GGATCC 1 cut(s) 749
BbrPI CACGTG 1 cut(s) 1034
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 1 cut(s) 913
BbvCI CCTCAGC 2 cut(s) 348, 1366
BbvI GCAGC 9 cut(s) 118, 226, 473, 1058, 1123, 1126, 1351, 1403, 1418
BccI CCATC 3 cut(s) 81, 655, 1154
BceAI ACGGC 1 cut(s) 1204
BciT130I CCWGG 3 cut(s) 666, 726, 1423
BciVI GTATCC 2 cut(s) 840, 1007
BcnI CCSGG 2 cut(s) 609, 969
BcoDI GTCTC 5 cut(s) 9, 60, 970, 1031, 1227
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 6 cut(s) 243, 642, 705, 1002, 1142, 1232
BfmI CTRYAG 3 cut(s) 274, 868, 1401
BfuI GTATCC 2 cut(s) 840, 1007
BisI GCNGC 9 cut(s) 132, 240, 462, 1047, 1112, 1115, 1340, 1392, 1407
BlsI GCNGC 9 cut(s) 133, 241, 463, 1048, 1113, 1116, 1341, 1393, 1408
Bme1390I CCNGG 5 cut(s) 609, 666, 726, 969, 1423
Bme18I GGWCC 1 cut(s) 1425
BmgT120I GGNCC 1 cut(s) 1425
BmiI GGNNCC 2 cut(s) 751, 1095
BmrFI CCNGG 5 cut(s) 609, 666, 726, 969, 1423
BmsI GCATC 3 cut(s) 334, 587, 1173
BoxI GACNNNNGTC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 596
Bpu10I CCTNAGC 2 cut(s) 348, 1366
BpuMI CCSGG 2 cut(s) 609, 969
BsaAI YACGTR 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 902
BsaJI CCNNGG 5 cut(s) 455, 665, 694, 968, 1283
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 818, 843, 1449
Bse1I ACTGG 4 cut(s) 269, 802, 930, 1134
Bse3DI GCAATG 2 cut(s) 223, 1064
Bse8I GATNNNNATC 1 cut(s) 91
BseBI CCWGG 3 cut(s) 666, 726, 1423
BseDI CCNNGG 5 cut(s) 455, 665, 694, 968, 1283
BseGI GGATG 2 cut(s) 675, 1165
BseJI GATNNNNATC 1 cut(s) 91
BseLI CCNNNNNNNGG 4 cut(s) 110, 818, 843, 1449
BseMI GCAATG 2 cut(s) 223, 1064
BseMII CTCAG 5 cut(s) 339, 436, 528, 759, 1380
BseNI ACTGG 4 cut(s) 269, 802, 930, 1134
BseRI GAGGAG 2 cut(s) 804, 988
BseXI GCAGC 9 cut(s) 118, 226, 473, 1058, 1123, 1126, 1351, 1403, 1418
Bsh1236I CGCG 2 cut(s) 457, 535
Bsh1285I CGRYCG 1 cut(s) 624
BshFI GGCC 3 cut(s) 646, 1057, 1133
BsiEI CGRYCG 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 913
BsiSI CCGG 2 cut(s) 609, 968
BslFI GGGAC 3 cut(s) 240, 771, 877
BslI CCNNNNNNNGG 4 cut(s) 110, 818, 843, 1449
BsmAI GTCTC 5 cut(s) 9, 60, 970, 1031, 1227
BsmBI CGTCTC 2 cut(s) 1031, 1227
BsmFI GGGAC 3 cut(s) 240, 771, 877
BsmI GAATGC 2 cut(s) 228, 1278
BsnI GGCC 3 cut(s) 646, 1057, 1133
Bsp1286I GDGCHC 1 cut(s) 913
Bsp143I GATC 5 cut(s) 29, 466, 625, 749, 926
Bsp19I CCATGG 1 cut(s) 1283
BspACI CCGC 4 cut(s) 440, 455, 457, 535
BspANI GGCC 3 cut(s) 646, 1057, 1133
BspCNI CTCAG 5 cut(s) 340, 435, 527, 758, 1379
BspFNI CGCG 2 cut(s) 457, 535
BspLI GGNNCC 2 cut(s) 751, 1095
BspPI GGATC 6 cut(s) 37, 461, 620, 744, 757, 921
BsrDI GCAATG 2 cut(s) 223, 1064
BsrI ACTGG 4 cut(s) 269, 802, 930, 1134
BssECI CCNNGG 5 cut(s) 455, 665, 694, 968, 1283
BssMI GATC 5 cut(s) 29, 466, 625, 749, 926
BssNI GRCGYC 1 cut(s) 902
BssT1I CCWWGG 2 cut(s) 694, 1283
Bst2UI CCWGG 3 cut(s) 666, 726, 1423
Bst4CI ACNGT 2 cut(s) 375, 852
BstACI GRCGYC 1 cut(s) 902
BstAPI GCANNNNNTGC 2 cut(s) 1330, 1415
BstBAI YACGTR 1 cut(s) 1034
BstC8I GCNNGC 3 cut(s) 459, 537, 1044
BstDEI CTNAG 5 cut(s) 348, 422, 514, 745, 1366
BstDSI CCRYGG 2 cut(s) 455, 1283
BstF5I GGATG 2 cut(s) 675, 1165
BstFNI CGCG 2 cut(s) 457, 535
BstHHI GCGC 2 cut(s) 450, 535
BstKTI GATC 5 cut(s) 32, 469, 628, 752, 929
BstMAI GTCTC 5 cut(s) 9, 60, 970, 1031, 1227
BstMBI GATC 5 cut(s) 29, 466, 625, 749, 926
BstMCI CGRYCG 1 cut(s) 624
BstNI CCWGG 3 cut(s) 666, 726, 1423
BstNSI RCATGY 1 cut(s) 786
BstPAI GACNNNNGTC 1 cut(s) 252
BstSCI CCNGG 5 cut(s) 607, 664, 724, 967, 1421
BstSFI CTRYAG 3 cut(s) 274, 868, 1401
BstUI CGCG 2 cut(s) 457, 535
BstV1I GCAGC 9 cut(s) 118, 226, 473, 1058, 1123, 1126, 1351, 1403, 1418
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 4 cut(s) 29, 466, 749, 926
BstYI RGATCY 4 cut(s) 29, 466, 749, 926
BsuI GTATCC 2 cut(s) 840, 1007
BsuRI GGCC 3 cut(s) 646, 1057, 1133
BtgI CCRYGG 2 cut(s) 455, 1283
BtsCI GGATG 2 cut(s) 675, 1165
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 3 cut(s) 459, 537, 1044
CfoI GCGC 2 cut(s) 450, 535
Cfr13I GGNCC 1 cut(s) 1425
Cfr42I CCGCGG 1 cut(s) 458
CseI GACGC 1 cut(s) 1048
CsiI ACCWGGT 1 cut(s) 1421
Csp6I GTAC 2 cut(s) 44, 801
CspCI CAANNNNNGTGG 4 cut(s) 97, 132, 1047, 1082
CviAII CATG 6 cut(s) 26, 128, 252, 596, 783, 1284
CviQI GTAC 2 cut(s) 44, 801
DdeI CTNAG 5 cut(s) 348, 422, 514, 745, 1366
DpnI GATC 5 cut(s) 31, 468, 627, 751, 928
DpnII GATC 5 cut(s) 29, 466, 625, 749, 926
EaeI YGGCCR 2 cut(s) 1055, 1131
Eco130I CCWWGG 2 cut(s) 694, 1283
Eco47I GGWCC 1 cut(s) 1425
Eco57I CTGAAG 1 cut(s) 174
Eco72I CACGTG 1 cut(s) 1034
EcoRII CCWGG 3 cut(s) 664, 724, 1421
EcoT14I CCWWGG 2 cut(s) 694, 1283
ErhI CCWWGG 2 cut(s) 694, 1283
Esp3I CGTCTC 2 cut(s) 1031, 1227
FaeI CATG 6 cut(s) 29, 131, 255, 599, 786, 1287
FaqI GGGAC 3 cut(s) 240, 771, 877
FatI CATG 6 cut(s) 25, 127, 251, 595, 782, 1283
FauI CCCGC 4 cut(s) 433, 450, 462, 528
Fnu4HI GCNGC 9 cut(s) 132, 240, 462, 1047, 1112, 1115, 1340, 1392, 1407
FokI GGATG 2 cut(s) 682, 1172
Fsp4HI GCNGC 9 cut(s) 132, 240, 462, 1047, 1112, 1115, 1340, 1392, 1407
FspBI CTAG 6 cut(s) 243, 642, 705, 1002, 1142, 1232
GlaI GCGC 2 cut(s) 449, 534
GluI GCNGC 9 cut(s) 132, 240, 462, 1047, 1112, 1115, 1340, 1392, 1407
HaeIII GGCC 3 cut(s) 646, 1057, 1133
HapII CCGG 2 cut(s) 609, 968
HgaI GACGC 1 cut(s) 1048
HhaI GCGC 2 cut(s) 450, 535
Hin1I GRCGYC 1 cut(s) 902
Hin1II CATG 6 cut(s) 29, 131, 255, 599, 786, 1287
Hin6I GCGC 2 cut(s) 448, 533
HinP1I GCGC 2 cut(s) 448, 533
HindIII AAGCTT 1 cut(s) 1196
HinfI GANTC 2 cut(s) 808, 822
HpaII CCGG 2 cut(s) 609, 968
Hpy188I TCNGA 5 cut(s) 154, 316, 425, 517, 636
Hpy188III TCNNGA 4 cut(s) 651, 747, 792, 1091
HpyAV CCTTC 5 cut(s) 115, 137, 269, 1004, 1014
HpyCH4III ACNGT 2 cut(s) 375, 852
HpyCH4IV ACGT 3 cut(s) 294, 902, 1033
HpyF3I CTNAG 5 cut(s) 348, 422, 514, 745, 1366
HpySE526I ACGT 3 cut(s) 294, 902, 1033
Hsp92I GRCGYC 1 cut(s) 902
Hsp92II CATG 6 cut(s) 29, 131, 255, 599, 786, 1287
HspAI GCGC 2 cut(s) 448, 533
KspI CCGCGG 1 cut(s) 458
Kzo9I GATC 5 cut(s) 29, 466, 625, 749, 926
LmnI GCTCC 2 cut(s) 298, 574
Lsp1109I GCAGC 9 cut(s) 118, 226, 473, 1058, 1123, 1126, 1351, 1403, 1418
LweI GCATC 3 cut(s) 334, 587, 1173
MabI ACCWGGT 1 cut(s) 1421
MaeI CTAG 6 cut(s) 243, 642, 705, 1002, 1142, 1232
MaeII ACGT 3 cut(s) 294, 902, 1033
MaeIII GTNAC 5 cut(s) 10, 245, 583, 672, 686
MalI GATC 5 cut(s) 31, 468, 627, 751, 928
MboI GATC 5 cut(s) 29, 466, 625, 749, 926
MboII GAAGA 1 cut(s) 596
MfeI CAATTG 2 cut(s) 135, 474
MflI RGATCY 4 cut(s) 29, 466, 749, 926
MhlI GDGCHC 1 cut(s) 913
MlsI TGGCCA 1 cut(s) 1057
MluCI AATT 8 cut(s) 135, 309, 474, 520, 655, 955, 1169, 1214
MluNI TGGCCA 1 cut(s) 1057
MmeI TCCRAC 2 cut(s) 38, 659
Mox20I TGGCCA 1 cut(s) 1057
MroXI GAANNNNTTC 2 cut(s) 603, 1024
MscI TGGCCA 1 cut(s) 1057
MseI TTAA 4 cut(s) 545, 882, 1104, 1301
MslI CAYNNNNRTG 1 cut(s) 598
Msp20I TGGCCA 1 cut(s) 1057
MspA1I CMGCKG 1 cut(s) 457
MspI CCGG 2 cut(s) 609, 968
MspR9I CCNGG 5 cut(s) 609, 666, 726, 969, 1423
MunI CAATTG 2 cut(s) 135, 474
Mva1269I GAATGC 2 cut(s) 228, 1278
MvaI CCWGG 3 cut(s) 666, 726, 1423
MvnI CGCG 2 cut(s) 457, 535
NciI CCSGG 2 cut(s) 609, 969
NcoI CCATGG 1 cut(s) 1283
NdeII GATC 5 cut(s) 29, 466, 625, 749, 926
NlaIII CATG 6 cut(s) 29, 131, 255, 599, 786, 1287
NlaIV GGNNCC 2 cut(s) 751, 1095
NmuCI GTSAC 3 cut(s) 10, 245, 672
NspI RCATGY 1 cut(s) 786
PctI GAATGC 2 cut(s) 228, 1278
PdmI GAANNNNTTC 2 cut(s) 603, 1024
PfeI GAWTC 2 cut(s) 808, 822
PkrI GCNGC 9 cut(s) 133, 241, 463, 1048, 1113, 1116, 1341, 1393, 1408
PmaCI CACGTG 1 cut(s) 1034
PmlI CACGTG 1 cut(s) 1034
Ppu21I YACGTR 1 cut(s) 1034
PshAI GACNNNNGTC 1 cut(s) 252
Psp6I CCWGG 3 cut(s) 664, 724, 1421
PspCI CACGTG 1 cut(s) 1034
PspGI CCWGG 3 cut(s) 664, 724, 1421
PspN4I GGNNCC 2 cut(s) 751, 1095
PspPI GGNCC 1 cut(s) 1425
PsrI GAACNNNNNNTAC 2 cut(s) 66, 98
PsuI RGATCY 4 cut(s) 29, 466, 749, 926
RsaI GTAC 2 cut(s) 45, 802
RsaNI GTAC 2 cut(s) 44, 801
RseI CAYNNNNRTG 1 cut(s) 598
SacII CCGCGG 1 cut(s) 458
SaqAI TTAA 4 cut(s) 545, 882, 1104, 1301
SatI GCNGC 9 cut(s) 132, 240, 462, 1047, 1112, 1115, 1340, 1392, 1407
Sau3AI GATC 5 cut(s) 29, 466, 625, 749, 926
Sau96I GGNCC 1 cut(s) 1425
ScrFI CCNGG 5 cut(s) 609, 666, 726, 969, 1423
SduI GDGCHC 1 cut(s) 913
SexAI ACCWGGT 1 cut(s) 1421
SfaNI GCATC 3 cut(s) 334, 587, 1173
SfcI CTRYAG 3 cut(s) 274, 868, 1401
Sfr303I CCGCGG 1 cut(s) 458
SgrBI CCGCGG 1 cut(s) 458
SinI GGWCC 1 cut(s) 1425
SmiMI CAYNNNNRTG 1 cut(s) 598
SpeI ACTAGT 1 cut(s) 1231
Sse9I AATT 8 cut(s) 135, 309, 474, 520, 655, 955, 1169, 1214
SsiI CCGC 4 cut(s) 440, 455, 457, 535
SspI AATATT 1 cut(s) 1121
SspMI CTAG 6 cut(s) 243, 642, 705, 1002, 1142, 1232
StyD4I CCNGG 5 cut(s) 607, 664, 724, 967, 1421
StyI CCWWGG 2 cut(s) 694, 1283
TaaI ACNGT 2 cut(s) 375, 852
TaiI ACGT 3 cut(s) 297, 905, 1036
TaqI TCGA 1 cut(s) 620
TaqII GACCGA 1 cut(s) 638
TasI AATT 8 cut(s) 135, 309, 474, 520, 655, 955, 1169, 1214
TfiI GAWTC 2 cut(s) 808, 822
Tru1I TTAA 4 cut(s) 545, 882, 1104, 1301
Tru9I TTAA 4 cut(s) 545, 882, 1104, 1301
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 3 cut(s) 10, 245, 672
TseI GCWGC 9 cut(s) 131, 239, 461, 1046, 1111, 1114, 1339, 1391, 1406
Tsp45I GTSAC 3 cut(s) 10, 245, 672
TspDTI ATGAA 1 cut(s) 612
TspGWI ACGGA 2 cut(s) 64, 876
TspRI CASTG 1 cut(s) 380
VpaK11BI GGWCC 1 cut(s) 1425
XapI RAATTY 5 cut(s) 309, 520, 655, 955, 1169
XceI RCATGY 1 cut(s) 786
XmnI GAANNNNTTC 2 cut(s) 603, 1024
XspI CTAG 6 cut(s) 243, 642, 705, 1002, 1142, 1232
ZraI GACGTC 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.