Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008868

Physical Location & Seq

Genomic Coordinates Reverse (-)

4504450 .. 4509662

Gene Structure 5213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g07501.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGGGGTCTTTGGAAGGTGAAGAAGCTGGAAGGAAGAAGGCAATGTGGCTCTACCCAAACCTTGCGGGAAGCGATCCTTCTGAAAGATGGGGGCACTCTGCTTGTTATTTTCAAGGGAATATGTATGTCTTTGGGGGGTGTTCTGGAGGTATGCATTACAGTGATGTTCAGGTGCTTAATTTTGAAACGATGGTCTGGAACACTCTTGTCACAACTGGTCCAGGGCCTGGTCCTAGAGATAGCCACAGTACCGTTCTTCTGGGTAATAATATGATAGTTTTTGGAGGTACAAGTGGCTCCAAGAAGTGTAATGATCTTCACATTTTAAATCTGATTACCAAAGAATGGACTCGACTAGAATGTGGCGGAACTCCACCTTCGCCTCGAGAAAGTCATTCTGCCACGCTTGTGGATGATGTAGAATTAGTGGTTTTTGGGGGAAGTGGGGAAGGTGAATGCTTGAATGATCTGCATGTTCTTGACCTTAGAACCATGAGATGGACTTCCCCTGAGGTGAAGGGTGATGTTCCTGTTCCTAGAGACAGTCATAGTGCTATTGCAATAGGAAATAAGATGTTTGTGTATGGTGGTGACCGTGGCGATAGGTATTATGGTGATGTTGATATGCTTGACATGGACACAATGACCTGGTCAAGGTTGGCTGTTCAAGGATCTTCACCCGGCGGCAGGGCTGGCCATGCAGCTGTGAGTATTGGCACAATGATCTATGTTATTGGAGGGGTCGGACACAAACGGTACTATAATGATGCATGGGTCCTGGATTCAAGTACTTGTTCATGGACCCAACTGGATGCACGCGGTCAACTGCCACAAGGGAGATTCTCTCACACTGCTGTTGTTACCGACTCTGTCCTTGCTATCTATGGAGGGTGTGGGGAGGATGATCGGCCTCTCAATGAGTTGTTGGTGTTGGAGCTTGGAACAGAACAGTCAAATGACTGCTACGATGTTCCTATATGCAAAATTTTTGGAAGCCCTATAAACCATGAGAGGAGAAGGTTCTCTAGGGGAGCTGATTTTAACACGAATCTGAAACCTATGCTTATGGGGAATCCTGTAGTAGTAAGACAGACATCTAAGAAATCAGAATCAGAAACAAAGCGACTTTTTTTAAAATCAGGTACTCTGCATCCGAAAAGGAGAAGAACTACCACTGCTAGAGCATGGGAGATTGAATCAGAACAAGAAGAGCGTTCTCTGTCTCTGTCCCAGCATTCATCATCATCTCACTCAGACCAAGAGCAGCAACCGACCCCAACTCCCAATCTGGAAGATTCCGCTCCACCTTCTCAAGGTTACGTCTTTAAGCAATTAAATCAGAGTCCGAACATAAAGATCTTAGAAATTCCGGCCAAAGATCTCAGATTTTCCACACAAAGAAGTCCAGAGGAACTTAGCCACTTGTTAGGAGCACATCAAATGAAAGCCAGACGAGAACAGTGTCTCCATGGTAGACCAAACATACAATTTCAAGCTGCAGAACGTGTACCATTTGTGGCAGTGAACCCTATGTGCAACTTGATTGGTTCTGAGGTCCATGGCAAAGTTGATGGTGCATTCGACTCCGGTTTCCTAATGACTGCAACTGTTAACGGGAGAATATTTAGAGGGGTCTTATTTGCACCGGGACGTGCGCTTGCCCCTAGAATGCCAGTTCCAGAGAATTCTTCATCTTTAACTAGTCACACTTGCAATCTGGGACCTGGAGTCATTGGAAGAGGGTCAATTGTTCCACAAAGTCAAATTGCCAATGCTGTGACACAACCGTTTGCAAACTCAAATCATGCAGAGTCATCCTCCTCAAAGCAATCCGAGCAGCCAACGAAGAATTTTTTACCAAGGTCTAGTCTAGGCCTCAGGCAGCCTCAGGTGGCTCGACCATTTCCAGGAGTTAGAACTTCATCTCTAGACAGAGAATCTAATCACCAAAGTGATTTTCAAGGTCTGGTTTTAACACTTGGTGGATCAGGAAGTGGTTCTGGTGGTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_3 Kelch_4

Protein Analysis

671

Amino Acids

72.89

Weight (kDa)

6.73

Isoelectric Point (pI)

54.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_HCF	PF13854	12 - 103	7.4e-13	Host cell factor, Kelch-repeats domain
Beta-prop_FBX42	PF13415	14 - 167	5.1e-37	FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	15 - 196	2.4e-59	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	53 - 250	3.4e-30	Attractin/LZTR1 beta-propeller
Kelch_HCF	PF13854	105 - 197	8.9e-18	Host cell factor, Kelch-repeats domain
Kelch_FKB95	PF25210	127 - 223	3e-06	FKB95, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	142 - 314	5.8e-51	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	144 - 298	1.3e-22	Attractin/LZTR1 beta-propeller
Beta-prop_FBX42	PF13415	152 - 316	3.2e-36	FBX42, beta-propeller domain
NANM	PF24996	175 - 299	1.1e-06	N-acetylneuraminate epimerase
Kelch_1	PF01344	179 - 221	1.9e-06	Kelch motif
Kelch_HCF	PF13854	194 - 247	9.1e-08	Host cell factor, Kelch-repeats domain
Kelch_1	PF01344	230 - 270	4.6e-10	Kelch motif
Beta-prop_ATRN-LZTR1	PF24981	242 - 317	1.6e-08	Attractin/LZTR1 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008868)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g07501 FvH4_6g07501
malus_domestica	MD00G1208300.v1.1 MD12G1205300.v1.1
prunus_persica	Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1
pyrus_communis	pycom04g17040 pycom12g19310 pycom12g19320
rosa_chinensis	RchiOBHm_Chr3g0457201
rosa_laevigata	RLG00000025243
rosa_multiflora	Rmu_co8495289.1_g000002 Rmu_sc0000488.1_g000018 Rmu_sc0016952.1_g000006
rosa_roxburghii	Rroxscaffold_6G00422120
rosa_rugosa	Rorug03G0019400
rosa_samantha	Rh3AG079200 Rh3BG080900 Rh3CG081100 Rh3DG082900
rosa_wichuraiana	Rw3G006820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	3 cut(s)	227, 345, 498
AccBSI	CCGCTC	1 cut(s)	1301
AccI	GTMKAC	1 cut(s)	1474
AccII	CGCG	1 cut(s)	819
AciI	CCGC	5 cut(s)	65, 366, 684, 819, 1299
AclWI	GGATC	3 cut(s)	68, 679, 1993
AcoI	YGGCCR	2 cut(s)	694, 1371
AcsI	RAATTY	4 cut(s)	984, 1365, 1684, 1849
AdeI	CACNNNGTG	1 cut(s)	1982
AfaI	GTAC	6 cut(s)	250, 289, 758, 790, 1144, 1509
AfiI	CCNNNNNNNGG	7 cut(s)	227, 345, 498, 654, 687, 1313, 1907
AflIII	ACRYGT	1 cut(s)	1504
AgsI	TTSAA	8 cut(s)	113, 185, 463, 668, 786, 1196, 1493, 1961
AhdI	GACNNNNNGTC	1 cut(s)	1727
AhlI	ACTAGT	1 cut(s)	1700
AjiI	CACGTC	1 cut(s)	1652
AjnI	CCWGG	6 cut(s)	220, 226, 647, 777, 1723, 1906
AjuI	GAANNNNNNNTTGG	2 cut(s)	1941, 1973
AleI	CACNNNNGTG	2 cut(s)	407, 1950
AloI	GAACNNNNNNTCC	2 cut(s)	1449, 1481
AluBI	AGCT	5 cut(s)	26, 704, 937, 1034, 1496
AluI	AGCT	5 cut(s)	26, 704, 937, 1034, 1496
Alw21I	GWGCWC	1 cut(s)	1435
Alw26I	GTCTC	3 cut(s)	534, 1227, 1469
AlwI	GGATC	3 cut(s)	68, 679, 1993
AlwNI	CAGNNNCTG	1 cut(s)	227
Ama87I	CYCGRG	1 cut(s)	384
AoxI	GGCC	5 cut(s)	224, 694, 908, 1371, 1873
ApeKI	GCWGC	5 cut(s)	701, 1264, 1496, 1837, 1882
ApoI	RAATTY	4 cut(s)	984, 1365, 1684, 1849
Asp700I	GAANNNNTTC	1 cut(s)	1996
AspLEI	GCGC	1 cut(s)	1657
AspS9I	GGNCC	7 cut(s)	218, 224, 230, 775, 801, 1555, 1721
AsuC2I	CCSGG	2 cut(s)	681, 1647
AsuHPI	GGTGA	8 cut(s)	29, 464, 526, 533, 602, 626, 669, 1937
AvaI	CYCGRG	1 cut(s)	384
AvaII	GGWCC	6 cut(s)	218, 230, 775, 801, 1555, 1721
AxyI	CCTNAGG	3 cut(s)	510, 1877, 1887
BaeGI	GKGCMC	1 cut(s)	96
BalI	TGGCCA	1 cut(s)	696
Bbv12I	GWGCWC	1 cut(s)	1435
BbvI	GCAGC	5 cut(s)	713, 1276, 1483, 1849, 1894
BccI	CCATC	4 cut(s)	81, 184, 492, 1565
BciT130I	CCWGG	6 cut(s)	222, 228, 649, 779, 1725, 1908
BcnI	CCSGG	2 cut(s)	681, 1647
BcoDI	GTCTC	3 cut(s)	534, 1227, 1469
BcuI	ACTAGT	1 cut(s)	1700
BfmI	CTRYAG	2 cut(s)	1077, 1497
BglII	AGATCT	2 cut(s)	1356, 1378
BisI	GCNGC	6 cut(s)	685, 702, 1265, 1497, 1838, 1883
BlsI	GCNGC	6 cut(s)	686, 703, 1266, 1498, 1839, 1884
BmcAI	AGTACT	1 cut(s)	790
Bme1390I	CCNGG	8 cut(s)	222, 228, 649, 681, 779, 1647, 1725, 1908
Bme18I	GGWCC	6 cut(s)	218, 230, 775, 801, 1555, 1721
BmeRI	GACNNNNNGTC	1 cut(s)	1727
BmeT110I	CYCGRG	1 cut(s)	384
BmgBI	CACGTC	1 cut(s)	1652
BmgT120I	GGNCC	7 cut(s)	218, 224, 230, 775, 801, 1555, 1721
BmiI	GGNNCC	4 cut(s)	298, 776, 803, 1722
BmrFI	CCNGG	8 cut(s)	222, 228, 649, 681, 779, 1647, 1725, 1908
BmsI	GCATC	3 cut(s)	757, 802, 1159
BplI	GAGNNNNNCTC	4 cut(s)	829, 861, 1802, 1834
BpmI	CTGGAG	2 cut(s)	165, 1746
BpuEI	CTTGAG	1 cut(s)	1296
BpuMI	CCSGG	2 cut(s)	681, 1647
BsaJI	CCNNGG	5 cut(s)	221, 595, 1468, 1558, 1859
BsaWI	WCCGGW	1 cut(s)	1586
BsaXI	ACNNNNNCTCC	4 cut(s)	1264, 1294, 1449, 1479
Bsc4I	CCNNNNNNNGG	7 cut(s)	227, 345, 498, 654, 687, 1313, 1907
Bse1I	ACTGG	3 cut(s)	220, 813, 1673
Bse21I	CCTNAGG	3 cut(s)	510, 1877, 1887
Bse3DI	GCAATG	1 cut(s)	48
BseBI	CCWGG	6 cut(s)	222, 228, 649, 779, 1725, 1908
BseDI	CCNNGG	5 cut(s)	221, 595, 1468, 1558, 1859
BseGI	GGATG	5 cut(s)	418, 817, 907, 1150, 1814
BseLI	CCNNNNNNNGG	7 cut(s)	227, 345, 498, 654, 687, 1313, 1907
BseMI	GCAATG	1 cut(s)	48
BseMII	CTCAG	6 cut(s)	501, 1266, 1396, 1542, 1891, 1901
BseNI	ACTGG	3 cut(s)	220, 813, 1673
BseRI	GAGGAG	2 cut(s)	1027, 1810
BseSI	GKGCMC	1 cut(s)	96
BseXI	GCAGC	5 cut(s)	713, 1276, 1483, 1849, 1894
BseYI	CCCAGC	1 cut(s)	1230
Bsh1236I	CGCG	1 cut(s)	819
BshFI	GGCC	5 cut(s)	226, 696, 910, 1373, 1875
BsiHKAI	GWGCWC	1 cut(s)	1435
BsiHKCI	CYCGRG	1 cut(s)	384
BsiSI	CCGG	4 cut(s)	681, 1370, 1587, 1646
BslFI	GGGAC	3 cut(s)	1213, 1662, 1734
BslI	CCNNNNNNNGG	7 cut(s)	227, 345, 498, 654, 687, 1313, 1907
BsmAI	GTCTC	3 cut(s)	534, 1227, 1469
BsmFI	GGGAC	3 cut(s)	1213, 1662, 1734
BsmI	GAATGC	4 cut(s)	461, 1234, 1577, 1674
BsnI	GGCC	5 cut(s)	226, 696, 910, 1373, 1875
BsoBI	CYCGRG	1 cut(s)	384
Bsp1286I	GDGCHC	2 cut(s)	96, 1435
Bsp143I	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
Bsp19I	CCATGG	2 cut(s)	1468, 1558
BspACI	CCGC	5 cut(s)	65, 366, 684, 819, 1299
BspANI	GGCC	5 cut(s)	226, 696, 910, 1373, 1875
BspCNI	CTCAG	6 cut(s)	502, 1265, 1395, 1543, 1890, 1900
BspFNI	CGCG	1 cut(s)	819
BspLI	GGNNCC	4 cut(s)	298, 776, 803, 1722
BspMAI	CTGCAG	1 cut(s)	1501
BspPI	GGATC	3 cut(s)	68, 679, 1993
BspQI	GCTCTTC	1 cut(s)	1203
BsrBI	CCGCTC	1 cut(s)	1301
BsrDI	GCAATG	1 cut(s)	48
BsrI	ACTGG	3 cut(s)	220, 813, 1673
BssECI	CCNNGG	5 cut(s)	221, 595, 1468, 1558, 1859
BssMI	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
BssT1I	CCWWGG	3 cut(s)	1468, 1558, 1859
Bst2UI	CCWGG	6 cut(s)	222, 228, 649, 779, 1725, 1908
Bst6I	CTCTTC	2 cut(s)	1203, 1732
BstC8I	GCNNGC	3 cut(s)	694, 817, 1659
BstDSI	CCRYGG	3 cut(s)	595, 1468, 1558
BstEII	GGTNACC	1 cut(s)	590
BstENI	CCTNNNNNAGG	2 cut(s)	652, 1311
BstF5I	GGATG	5 cut(s)	418, 817, 907, 1150, 1814
BstFNI	CGCG	1 cut(s)	819
BstHHI	GCGC	1 cut(s)	1657
BstKTI	GATC	9 cut(s)	76, 316, 469, 674, 726, 907, 1359, 1381, 1988
BstMAI	GTCTC	3 cut(s)	534, 1227, 1469
BstMBI	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
BstMWI	GCNNNNNNNGC	3 cut(s)	693, 698, 1834
BstNI	CCWGG	6 cut(s)	222, 228, 649, 779, 1725, 1908
BstNSI	RCATGY	1 cut(s)	476
BstPI	GGTNACC	1 cut(s)	590
BstSCI	CCNGG	8 cut(s)	220, 226, 647, 679, 777, 1645, 1723, 1906
BstSFI	CTRYAG	2 cut(s)	1077, 1497
BstSLI	GKGCMC	1 cut(s)	96
BstUI	CGCG	1 cut(s)	819
BstV1I	GCAGC	5 cut(s)	713, 1276, 1483, 1849, 1894
BstX2I	RGATCY	3 cut(s)	671, 1356, 1378
BstXI	CCANNNNNNTGG	1 cut(s)	409
BstYI	RGATCY	3 cut(s)	671, 1356, 1378
Bsu36I	CCTNAGG	3 cut(s)	510, 1877, 1887
BsuRI	GGCC	5 cut(s)	226, 696, 910, 1373, 1875
BtgI	CCRYGG	3 cut(s)	595, 1468, 1558
BtrI	CACGTC	1 cut(s)	1652
BtsCI	GGATG	5 cut(s)	418, 817, 907, 1150, 1814
BtsI	GCAGTG	3 cut(s)	849, 1173, 1527
BtsIMutI	CAGTG	5 cut(s)	166, 849, 1173, 1466, 1527
Cac8I	GCNNGC	3 cut(s)	694, 817, 1659
CaiI	CAGNNNCTG	1 cut(s)	227
CfoI	GCGC	1 cut(s)	1657
Cfr13I	GGNCC	7 cut(s)	218, 224, 230, 775, 801, 1555, 1721
CsiI	ACCWGGT	1 cut(s)	647
Csp6I	GTAC	6 cut(s)	249, 288, 757, 789, 1143, 1508
CviQI	GTAC	6 cut(s)	249, 288, 757, 789, 1143, 1508
DpnI	GATC	9 cut(s)	75, 315, 468, 673, 725, 906, 1358, 1380, 1987
DpnII	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
DraI	TTTAAA	2 cut(s)	327, 1134
DraIII	CACNNNGTG	1 cut(s)	1982
DriI	GACNNNNNGTC	1 cut(s)	1727
EaeI	YGGCCR	2 cut(s)	694, 1371
Eam1104I	CTCTTC	2 cut(s)	1203, 1732
Eam1105I	GACNNNNNGTC	1 cut(s)	1727
EarI	CTCTTC	2 cut(s)	1203, 1732
EciI	GGCGGA	1 cut(s)	381
Eco130I	CCWWGG	3 cut(s)	1468, 1558, 1859
Eco147I	AGGCCT	1 cut(s)	1875
Eco47I	GGWCC	6 cut(s)	218, 230, 775, 801, 1555, 1721
Eco81I	CCTNAGG	3 cut(s)	510, 1877, 1887
Eco88I	CYCGRG	1 cut(s)	384
Eco91I	GGTNACC	1 cut(s)	590
EcoNI	CCTNNNNNAGG	2 cut(s)	652, 1311
EcoO109I	RGGNCCY	3 cut(s)	224, 775, 1721
EcoO65I	GGTNACC	1 cut(s)	590
EcoRI	GAATTC	1 cut(s)	1684
EcoRII	CCWGG	6 cut(s)	220, 226, 647, 777, 1723, 1906
EcoT14I	CCWWGG	3 cut(s)	1468, 1558, 1859
EcoT22I	ATGCAT	2 cut(s)	156, 772
ErhI	CCWWGG	3 cut(s)	1468, 1558, 1859
FalI	AAGNNNNNCTT	2 cut(s)	61, 93
FaqI	GGGAC	3 cut(s)	1213, 1662, 1734
FauI	CCCGC	1 cut(s)	58
FblI	GTMKAC	1 cut(s)	1474
Fnu4HI	GCNGC	6 cut(s)	685, 702, 1265, 1497, 1838, 1883
FokI	GGATG	5 cut(s)	425, 824, 914, 1137, 1801
Fsp4HI	GCNGC	6 cut(s)	685, 702, 1265, 1497, 1838, 1883
GlaI	GCGC	1 cut(s)	1656
GluI	GCNGC	6 cut(s)	685, 702, 1265, 1497, 1838, 1883
GsaI	CCCAGC	1 cut(s)	1234
GsuI	CTGGAG	2 cut(s)	165, 1746
HaeIII	GGCC	5 cut(s)	226, 696, 910, 1373, 1875
HapII	CCGG	4 cut(s)	681, 1370, 1587, 1646
HhaI	GCGC	1 cut(s)	1657
Hin6I	GCGC	1 cut(s)	1655
HinP1I	GCGC	1 cut(s)	1655
HincII	GTYRAC	2 cut(s)	824, 1612
HindII	GTYRAC	2 cut(s)	824, 1612
HpaI	GTTAAC	1 cut(s)	1612
HpaII	CCGG	4 cut(s)	681, 1370, 1587, 1646
HphI	GGTGA	8 cut(s)	29, 464, 526, 533, 602, 626, 669, 1937
Hpy166II	GTNNAC	5 cut(s)	824, 1475, 1508, 1525, 1612
Hpy188III	TCNNGA	9 cut(s)	144, 196, 386, 479, 1289, 1406, 1679, 1928, 1989
Hpy8I	GTNNAC	5 cut(s)	824, 1475, 1508, 1525, 1612
HpyAV	CCTTC	9 cut(s)	8, 24, 31, 87, 387, 443, 511, 1011, 1317
HpyCH4IV	ACGT	3 cut(s)	1320, 1504, 1651
HpyF10VI	GCNNNNNNNGC	3 cut(s)	693, 698, 1834
HpySE526I	ACGT	3 cut(s)	1320, 1504, 1651
HspAI	GCGC	1 cut(s)	1655
KspAI	GTTAAC	1 cut(s)	1612
Kzo9I	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
LguI	GCTCTTC	1 cut(s)	1203
LmnI	GCTCC	5 cut(s)	302, 934, 1031, 1306, 1430
Lsp1109I	GCAGC	5 cut(s)	713, 1276, 1483, 1849, 1894
LweI	GCATC	3 cut(s)	757, 802, 1159
MabI	ACCWGGT	1 cut(s)	647
MaeII	ACGT	3 cut(s)	1320, 1504, 1651
MaeIII	GTNAC	6 cut(s)	208, 590, 859, 1316, 1703, 1777
MalI	GATC	9 cut(s)	75, 315, 468, 673, 725, 906, 1358, 1380, 1987
MbiI	CCGCTC	1 cut(s)	1301
MboI	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
MfeI	CAATTG	1 cut(s)	1746
MflI	RGATCY	3 cut(s)	671, 1356, 1378
MhlI	GDGCHC	2 cut(s)	96, 1435
MlsI	TGGCCA	1 cut(s)	696
MluNI	TGGCCA	1 cut(s)	696
MlyI	GAGTC	6 cut(s)	343, 860, 1351, 1577, 1737, 1820
MmeI	TCCRAC	2 cut(s)	724, 912
Mox20I	TGGCCA	1 cut(s)	696
Mph1103I	ATGCAT	2 cut(s)	156, 772
MroXI	GAANNNNTTC	1 cut(s)	1996
MscI	TGGCCA	1 cut(s)	696
MseI	TTAA	9 cut(s)	177, 326, 1041, 1133, 1326, 1334, 1611, 1697, 1973
MslI	CAYNNNNRTG	3 cut(s)	159, 407, 1950
Msp20I	TGGCCA	1 cut(s)	696
MspA1I	CMGCKG	1 cut(s)	704
MspI	CCGG	4 cut(s)	681, 1370, 1587, 1646
MspR9I	CCNGG	8 cut(s)	222, 228, 649, 681, 779, 1647, 1725, 1908
MunI	CAATTG	1 cut(s)	1746
Mva1269I	GAATGC	4 cut(s)	461, 1234, 1577, 1674
MvaI	CCWGG	6 cut(s)	222, 228, 649, 779, 1725, 1908
MvnI	CGCG	1 cut(s)	819
MwoI	GCNNNNNNNGC	3 cut(s)	693, 698, 1834
NciI	CCSGG	2 cut(s)	681, 1647
NcoI	CCATGG	2 cut(s)	1468, 1558
NdeII	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
NlaIV	GGNNCC	4 cut(s)	298, 776, 803, 1722
NmuCI	GTSAC	4 cut(s)	208, 590, 1703, 1777
NsiI	ATGCAT	2 cut(s)	156, 772
NspI	RCATGY	1 cut(s)	476
OliI	CACNNNNGTG	2 cut(s)	407, 1950
PaeR7I	CTCGAG	1 cut(s)	384
PceI	AGGCCT	1 cut(s)	1875
PciSI	GCTCTTC	1 cut(s)	1203
PctI	GAATGC	4 cut(s)	461, 1234, 1577, 1674
PdmI	GAANNNNTTC	1 cut(s)	1996
PfeI	GAWTC	8 cut(s)	782, 840, 1048, 1072, 1109, 1196, 1295, 1937
PflFI	GACNNNGTC	2 cut(s)	649, 869
PflMI	CCANNNNNTGG	3 cut(s)	227, 345, 498
PfoI	TCCNGGA	2 cut(s)	777, 1906
PkrI	GCNGC	6 cut(s)	686, 703, 1266, 1498, 1839, 1884
PleI	GAGTC	6 cut(s)	343, 860, 1350, 1577, 1736, 1819
PpsI	GAGTC	6 cut(s)	343, 860, 1350, 1577, 1736, 1819
PpuMI	RGGWCCY	2 cut(s)	775, 1721
Psp5II	RGGWCCY	2 cut(s)	775, 1721
Psp6I	CCWGG	6 cut(s)	220, 226, 647, 777, 1723, 1906
PspEI	GGTNACC	1 cut(s)	590
PspFI	CCCAGC	1 cut(s)	1230
PspGI	CCWGG	6 cut(s)	220, 226, 647, 777, 1723, 1906
PspN4I	GGNNCC	4 cut(s)	298, 776, 803, 1722
PspPI	GGNCC	7 cut(s)	218, 224, 230, 775, 801, 1555, 1721
PspPPI	RGGWCCY	2 cut(s)	775, 1721
PstI	CTGCAG	1 cut(s)	1501
PstNI	CAGNNNCTG	1 cut(s)	227
PsuI	RGATCY	3 cut(s)	671, 1356, 1378
PsyI	GACNNNGTC	2 cut(s)	649, 869
PvuII	CAGCTG	1 cut(s)	704
RsaI	GTAC	6 cut(s)	250, 289, 758, 790, 1144, 1509
RsaNI	GTAC	6 cut(s)	249, 288, 757, 789, 1143, 1508
RseI	CAYNNNNRTG	3 cut(s)	159, 407, 1950
SapI	GCTCTTC	1 cut(s)	1203
SaqAI	TTAA	9 cut(s)	177, 326, 1041, 1133, 1326, 1334, 1611, 1697, 1973
SatI	GCNGC	6 cut(s)	685, 702, 1265, 1497, 1838, 1883
Sau3AI	GATC	9 cut(s)	73, 313, 466, 671, 723, 904, 1356, 1378, 1985
Sau96I	GGNCC	7 cut(s)	218, 224, 230, 775, 801, 1555, 1721
ScaI	AGTACT	1 cut(s)	790
SchI	GAGTC	6 cut(s)	343, 860, 1351, 1577, 1737, 1820
ScrFI	CCNGG	8 cut(s)	222, 228, 649, 681, 779, 1647, 1725, 1908
SduI	GDGCHC	2 cut(s)	96, 1435
SexAI	ACCWGGT	1 cut(s)	647
SfaNI	GCATC	3 cut(s)	757, 802, 1159
SfcI	CTRYAG	2 cut(s)	1077, 1497
Sfr274I	CTCGAG	1 cut(s)	384
SinI	GGWCC	6 cut(s)	218, 230, 775, 801, 1555, 1721
SlaI	CTCGAG	1 cut(s)	384
SmiMI	CAYNNNNRTG	3 cut(s)	159, 407, 1950
SmlI	CTYRAG	2 cut(s)	384, 1311
SmoI	CTYRAG	2 cut(s)	384, 1311
SpeI	ACTAGT	1 cut(s)	1700
SseBI	AGGCCT	1 cut(s)	1875
SsiI	CCGC	5 cut(s)	65, 366, 684, 819, 1299
SspI	AATATT	1 cut(s)	1623
StuI	AGGCCT	1 cut(s)	1875
StyD4I	CCNGG	8 cut(s)	220, 226, 647, 679, 777, 1645, 1723, 1906
StyI	CCWWGG	3 cut(s)	1468, 1558, 1859
TaiI	ACGT	3 cut(s)	1323, 1507, 1654
TaqI	TCGA	4 cut(s)	352, 385, 1581, 1897
TatI	WGTACW	1 cut(s)	788
TauI	GCSGC	1 cut(s)	687
TfiI	GAWTC	8 cut(s)	782, 840, 1048, 1072, 1109, 1196, 1295, 1937
Tru1I	TTAA	9 cut(s)	177, 326, 1041, 1133, 1326, 1334, 1611, 1697, 1973
Tru9I	TTAA	9 cut(s)	177, 326, 1041, 1133, 1326, 1334, 1611, 1697, 1973
TscAI	CASTG	5 cut(s)	166, 856, 1180, 1466, 1527
TseFI	GTSAC	4 cut(s)	208, 590, 1703, 1777
TseI	GCWGC	5 cut(s)	701, 1264, 1496, 1837, 1882
Tsp45I	GTSAC	4 cut(s)	208, 590, 1703, 1777
TspDTI	ATGAA	5 cut(s)	786, 1227, 1457, 1680, 1911
TspRI	CASTG	5 cut(s)	166, 856, 1180, 1466, 1527
Tth111I	GACNNNGTC	2 cut(s)	649, 869
Van91I	CCANNNNNTGG	3 cut(s)	227, 345, 498
VpaK11BI	GGWCC	6 cut(s)	218, 230, 775, 801, 1555, 1721
XagI	CCTNNNNNAGG	2 cut(s)	652, 1311
XapI	RAATTY	4 cut(s)	984, 1365, 1684, 1849
XbaI	TCTAGA	1 cut(s)	1927
XceI	RCATGY	1 cut(s)	476
XhoI	CTCGAG	1 cut(s)	384
XmiI	GTMKAC	1 cut(s)	1474
XmnI	GAANNNNTTC	1 cut(s)	1996
ZrmI	AGTACT	1 cut(s)	790
Zsp2I	ATGCAT	2 cut(s)	156, 772

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.