Rw3G006820

Kelch motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008868
Physical Location & Seq
Reverse (-)
6352475 .. 6356012
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006820.1

Sequence Viewer

Length: 1995 bp
ATGGGGTCTTTGGGAGGTGAAGAAGCTACCAGGAAGAAGGCAATGTGGCTCTACCCAAAGCTTGTGGGATTCAATCCTTCTGAAAGATGGGGGCACTCTGCTTGTTATTTTCAAGGGAATATGTATGTTTTTGGGGGATGTTCTGGAGGTATGCATTACAGTGATGTTCTTGTGCTTAATTATGAAACGATGGCCTGGAACACTCTTGTAACAACTGGTAAAGGGCCTGGTCCTAGGGATAGCCACAGTACCGTTGTTCTGGGTAATAATATGATAGTTTTTGGGGGTACAAGTGGCTCCAAGAAGTGTAATGATCTTCACATTTTAAATCTGATGACCAAAGAATGGACTCGACCAGAATGTGGCGGAACTCCACCTTCGCCCCGAGAAAGTCATTCTGCCACGCTTGTGGATGATGAAGAATTAGTGGTTTTTGGGGGAAGTGGAGAAGGTCAAAGCTTGAATGATCTGCATGTTCTAGACCTCAAAACCATGAGATGGACTTCCCCTGAGGTGAAGGGTGATATTCCTGTTCCTAGAGACAGTCATAGTGCTATTGCAATAGGAAAGAAGATGCTTGTGTACGGTGGTGACCGTGGTGATAGGTATTATGGTGATGTTGATATGCTTGACATGGACACAATGACTTGGTCAAGGTTGGCTGTTCAAGGATCTTCACCCGGTGGCAGGGCTGGTCATGCAGCTGTGAGTATTGGCACAAAGGTCTATGTCATTGGAGGGGTTGGACACAAACGGTACTACAATGATGCATGGGTCCTGGACTCAAGTACTTGTTCATGGACCCAACTCGATGTATGCGGCCAACTGCCACAAGGGAGGTTCTCTCACACTGCTGTTGTTACCGACTCTGACATTGCCGTCTATGGAGGGTGTGGGGAGGATGATCGTCCTCTCGATGAGTTGTTGGTGTTGCAGCTTGGAACAGAGCTGCCAAATGAGTGTTACAATGTTCCTATGTGCAAAATTTTTGGAAGCCATTTAAACCAAGAGAGGAGAAGGTTCTCTAGGGGAGCTGAATTTAACACGAATGTGAAACCTATGCTTATGGGGAATCCTGTAGTGGTAAGACAGACATCCAAGATATCAGAATCAGAAACAAAGCGTTCATCTTTAAAATCAGATACTCTGCATCCAAAAAGGAGAAGAAGTACCACTTTGAAAGCATGGGATGTTGAATCAGAACAAGAAGAGCATTCTCTATCTCTGTCCCAGCATTCATCATCATCTCACTCAGACCAAGAACAGCAACAGACCCCAACTCCCAAACTTGAAGATTCTGCCCCACCTTCTAAAGGTTTTCATATCTTTAAGCAATTAAATCAGAGTCCAAACATAAAGATCTTAGAAATTCCAGCCAAAGATCTCAGAATTTCCACGCAAAGAACTCCAGAGGAACTTAGCCACTTGTTAGGAGCACATCAAATGAAAGCGAGACGAGAACAGTATCTTCATGGTAGACCAAGCATGCACTTTCAAGCTGCAGGGCCTATGCAGAACCCGATTGGTGCTGAGGTCCATGGCAAAGTTGATGGAGCATTTGACTCCGGTTTCCTAATGACTGCAACTGTTAACGGAAATATATATAGAGGGGTCTTATTTGCACCGGGAGCTGGGATCGTCCCAAGAATGTCAATTCTTCCACAAAATTCTTCATCTTTAACAAGTCAAACTGGCAATCTGGGACCTGGAGTCATTGGAAGAGGGTCAATTGTTCCACAAAGTCAAATAGCCAATGCTGTGACACAACCCTTTGCAAACTCAAATCATGCAGAGCCATCCTCCTCAAAGCTATCCGAGCAGCCAATGAAGAATTCCCTACCAGGGTCTAGTCTAGGCCTCAGACAACCTCAGGTGGCTCGGCCATTTTCAGGAGTTAGTGCTTCATCTTTAGTCAGAGAACCTAATCACCGAAATGATTTTCAAGGTCTGGTTCTAACACTTGGAGGATCAGGAAGTGGCTCTGGAGGTTTGTAG

Protein Analysis

664

Amino Acids

71.91

Weight (kDa)

7.35

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_HCF PF13854 12 - 97 1.1e-12 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 14 - 197 1.2e-60 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 14 - 167 6.7e-39 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 53 - 197 9.5e-23 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 78 - 118 1.7e-06 Kelch motif
Kelch_FKB95 PF25210 79 - 199 2.3e-06 FKB95, Kelch-repeats domain
Kelch_HCF PF13854 104 - 197 4.1e-18 Host cell factor, Kelch-repeats domain
Beta-prop_TYW4 PF13418 126 - 217 7.4e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 141 - 297 4.8e-50 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 143 - 299 6.2e-23 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 151 - 315 5e-36 FBX42, beta-propeller domain
NANM PF24996 175 - 299 3.9e-06 N-acetylneuraminate epimerase
Kelch_HCF PF13854 191 - 247 1.7e-09 Host cell factor, Kelch-repeats domain
Beta-prop_TYW4 PF13418 227 - 353 6.4e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_FKB95 PF25210 229 - 303 3.2e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 230 - 270 1.7e-10 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AccB7I CCANNNNNTGG 3 cut(s) 345, 362, 498
AccI GTMKAC 1 cut(s) 1477
AciI CCGC 2 cut(s) 366, 819
AclWI GGATC 3 cut(s) 679, 1643, 1975
AcoI YGGCCR 2 cut(s) 820, 1880
AcsI RAATTY 6 cut(s) 984, 1037, 1368, 1389, 1666, 1831
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 6 cut(s) 250, 289, 584, 758, 790, 1171
AfiI CCNNNNNNNGG 8 cut(s) 345, 362, 498, 687, 1313, 1631, 1842, 1889
AgsI TTSAA 9 cut(s) 73, 113, 463, 668, 1180, 1196, 1292, 1496, 1943
AhdI GACNNNNNGTC 1 cut(s) 1709
AjnI CCWGG 6 cut(s) 29, 194, 226, 777, 1705, 1840
AjuI GAANNNNNNNTTGG 2 cut(s) 1816, 1848
AleI CACNNNNGTG 2 cut(s) 407, 1049
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 2 cut(s) 534, 1447
AlwI GGATC 3 cut(s) 679, 1643, 1975
Ama87I CYCGRG 1 cut(s) 384
AoxI GGCC 6 cut(s) 192, 224, 820, 1505, 1855, 1880
ApeKI GCWGC 5 cut(s) 701, 934, 949, 1499, 1819
ApoI RAATTY 6 cut(s) 984, 1037, 1368, 1389, 1666, 1831
AspA2I CCTAGG 1 cut(s) 233
AspS9I GGNCC 7 cut(s) 224, 230, 775, 801, 1505, 1534, 1703
AsuC2I CCSGG 2 cut(s) 681, 1626
AsuHPI GGTGA 8 cut(s) 29, 526, 533, 602, 611, 626, 669, 1919
AvaI CYCGRG 1 cut(s) 384
AvaII GGWCC 5 cut(s) 230, 775, 801, 1534, 1703
AvrII CCTAGG 1 cut(s) 233
AxyI CCTNAGG 2 cut(s) 510, 1869
BaeGI GKGCMC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 1438
BbvCI CCTCAGC 1 cut(s) 1530
BbvI GCAGC 5 cut(s) 713, 936, 946, 1486, 1831
BccI CCATC 5 cut(s) 81, 184, 492, 1544, 1804
BceAI ACGGC 1 cut(s) 863
BciT130I CCWGG 6 cut(s) 31, 196, 228, 779, 1707, 1842
BcnI CCSGG 2 cut(s) 681, 1626
BcoDI GTCTC 2 cut(s) 534, 1447
BfaI CTAG 6 cut(s) 234, 479, 537, 1026, 1848, 1853
BfmI CTRYAG 2 cut(s) 1077, 1500
BglII AGATCT 2 cut(s) 1359, 1381
BisI GCNGC 6 cut(s) 702, 820, 935, 950, 1500, 1820
BlnI CCTAGG 1 cut(s) 233
BlsI GCNGC 6 cut(s) 703, 821, 936, 951, 1501, 1821
BmcAI AGTACT 1 cut(s) 790
Bme1390I CCNGG 8 cut(s) 31, 196, 228, 681, 779, 1626, 1707, 1842
Bme18I GGWCC 5 cut(s) 230, 775, 801, 1534, 1703
BmeRI GACNNNNNGTC 1 cut(s) 1709
BmeT110I CYCGRG 1 cut(s) 384
BmgT120I GGNCC 7 cut(s) 224, 230, 775, 801, 1505, 1534, 1703
BmiI GGNNCC 4 cut(s) 298, 776, 803, 1704
BmrFI CCNGG 8 cut(s) 31, 196, 228, 681, 779, 1626, 1707, 1842
BmsI GCATC 3 cut(s) 564, 757, 1159
BplI GAGNNNNNCTC 4 cut(s) 829, 861, 1784, 1816
BpmI CTGGAG 3 cut(s) 165, 1392, 1728
Bpu10I CCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 1 cut(s) 769
BpuMI CCSGG 2 cut(s) 681, 1626
BsaJI CCNNGG 4 cut(s) 233, 595, 1537, 1841
BsaWI WCCGGW 1 cut(s) 1565
BsaXI ACNNNNNCTCC 4 cut(s) 1153, 1183, 1264, 1294
Bsc4I CCNNNNNNNGG 8 cut(s) 345, 362, 498, 687, 1313, 1631, 1842, 1889
Bse1I ACTGG 2 cut(s) 220, 1696
Bse21I CCTNAGG 2 cut(s) 510, 1869
Bse3DI GCAATG 2 cut(s) 48, 873
BseBI CCWGG 6 cut(s) 31, 196, 228, 779, 1707, 1842
BseDI CCNNGG 4 cut(s) 233, 595, 1537, 1841
BseGI GGATG 7 cut(s) 143, 418, 907, 1094, 1150, 1195, 1796
BseLI CCNNNNNNNGG 8 cut(s) 345, 362, 498, 687, 1313, 1631, 1842, 1889
BseMI GCAATG 2 cut(s) 48, 873
BseMII CTCAG 6 cut(s) 501, 1266, 1399, 1521, 1873, 1883
BseNI ACTGG 2 cut(s) 220, 1696
BseRI GAGGAG 2 cut(s) 1027, 1792
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 5 cut(s) 713, 936, 946, 1486, 1831
BseYI CCCAGC 2 cut(s) 1230, 1631
BshFI GGCC 6 cut(s) 194, 226, 822, 1507, 1857, 1882
BsiHKAI GWGCWC 1 cut(s) 1438
BsiHKCI CYCGRG 1 cut(s) 384
BsiSI CCGG 3 cut(s) 681, 1566, 1625
BslFI GGGAC 3 cut(s) 1213, 1625, 1716
BslI CCNNNNNNNGG 8 cut(s) 345, 362, 498, 687, 1313, 1631, 1842, 1889
BsmAI GTCTC 2 cut(s) 534, 1447
BsmBI CGTCTC 1 cut(s) 1447
BsmFI GGGAC 3 cut(s) 1213, 1625, 1716
BsmI GAATGC 2 cut(s) 1213, 1234
BsnI GGCC 6 cut(s) 194, 226, 822, 1507, 1857, 1882
BsoBI CYCGRG 1 cut(s) 384
Bsp1286I GDGCHC 2 cut(s) 96, 1438
Bsp143I GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
Bsp19I CCATGG 1 cut(s) 1537
BspACI CCGC 2 cut(s) 366, 819
BspANI GGCC 6 cut(s) 194, 226, 822, 1507, 1857, 1882
BspCNI CTCAG 6 cut(s) 502, 1265, 1398, 1522, 1872, 1882
BspLI GGNNCC 4 cut(s) 298, 776, 803, 1704
BspMAI CTGCAG 1 cut(s) 1504
BspPI GGATC 3 cut(s) 679, 1643, 1975
BspQI GCTCTTC 1 cut(s) 1203
BsrDI GCAATG 2 cut(s) 48, 873
BsrI ACTGG 2 cut(s) 220, 1696
BssECI CCNNGG 4 cut(s) 233, 595, 1537, 1841
BssMI GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
BssT1I CCWWGG 2 cut(s) 233, 1537
Bst2UI CCWGG 6 cut(s) 31, 196, 228, 779, 1707, 1842
Bst4CI ACNGT 9 cut(s) 161, 248, 253, 545, 587, 596, 756, 1464, 1588
Bst6I CTCTTC 2 cut(s) 1203, 1714
BstC8I GCNNGC 1 cut(s) 1487
BstDEI CTNAG 8 cut(s) 510, 1252, 1363, 1385, 1418, 1530, 1859, 1869
BstDSI CCRYGG 2 cut(s) 595, 1537
BstEII GGTNACC 1 cut(s) 590
BstENI CCTNNNNNAGG 1 cut(s) 1311
BstF5I GGATG 7 cut(s) 143, 418, 907, 1094, 1150, 1195, 1796
BstKTI GATC 8 cut(s) 316, 469, 674, 907, 1362, 1384, 1638, 1970
BstMAI GTCTC 2 cut(s) 534, 1447
BstMBI GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
BstMWI GCNNNNNNNGC 3 cut(s) 698, 1628, 1816
BstNI CCWGG 6 cut(s) 31, 196, 228, 779, 1707, 1842
BstNSI RCATGY 2 cut(s) 476, 1489
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 8 cut(s) 29, 194, 226, 679, 777, 1624, 1705, 1840
BstSFI CTRYAG 2 cut(s) 1077, 1500
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 713, 936, 946, 1486, 1831
BstX2I RGATCY 3 cut(s) 671, 1359, 1381
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 3 cut(s) 671, 1359, 1381
Bsu36I CCTNAGG 2 cut(s) 510, 1869
BsuRI GGCC 6 cut(s) 194, 226, 822, 1507, 1857, 1882
BtgI CCRYGG 2 cut(s) 595, 1537
BtsCI GGATG 7 cut(s) 143, 418, 907, 1094, 1150, 1195, 1796
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 166, 849
Cac8I GCNNGC 1 cut(s) 1487
Cfr13I GGNCC 7 cut(s) 224, 230, 775, 801, 1505, 1534, 1703
Csp6I GTAC 6 cut(s) 249, 288, 583, 757, 789, 1170
CspCI CAANNNNNGTGG 2 cut(s) 45, 80
CviQI GTAC 6 cut(s) 249, 288, 583, 757, 789, 1170
DdeI CTNAG 8 cut(s) 510, 1252, 1363, 1385, 1418, 1530, 1859, 1869
DpnI GATC 8 cut(s) 315, 468, 673, 906, 1361, 1383, 1637, 1969
DpnII GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
DraI TTTAAA 3 cut(s) 327, 1002, 1134
DraIII CACNNNGTG 1 cut(s) 683
DrdI GACNNNNNNGTC 1 cut(s) 878
DriI GACNNNNNGTC 1 cut(s) 1709
DseDI GACNNNNNNGTC 1 cut(s) 878
EaeI YGGCCR 2 cut(s) 820, 1880
Eam1104I CTCTTC 2 cut(s) 1203, 1714
Eam1105I GACNNNNNGTC 1 cut(s) 1709
EarI CTCTTC 2 cut(s) 1203, 1714
EciI GGCGGA 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 233, 1537
Eco147I AGGCCT 1 cut(s) 1857
Eco32I GATATC 1 cut(s) 1104
Eco47I GGWCC 5 cut(s) 230, 775, 801, 1534, 1703
Eco81I CCTNAGG 2 cut(s) 510, 1869
Eco88I CYCGRG 1 cut(s) 384
Eco91I GGTNACC 1 cut(s) 590
EcoNI CCTNNNNNAGG 1 cut(s) 1311
EcoO109I RGGNCCY 4 cut(s) 224, 775, 1505, 1703
EcoO65I GGTNACC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 1831
EcoRII CCWGG 6 cut(s) 29, 194, 226, 777, 1705, 1840
EcoRV GATATC 1 cut(s) 1104
EcoT14I CCWWGG 2 cut(s) 233, 1537
EcoT22I ATGCAT 2 cut(s) 156, 772
ErhI CCWWGG 2 cut(s) 233, 1537
Esp3I CGTCTC 1 cut(s) 1447
FalI AAGNNNNNCTT 2 cut(s) 1159, 1191
FaqI GGGAC 3 cut(s) 1213, 1625, 1716
FblI GTMKAC 1 cut(s) 1477
Fnu4HI GCNGC 6 cut(s) 702, 820, 935, 950, 1500, 1820
FokI GGATG 7 cut(s) 150, 425, 914, 1081, 1137, 1202, 1783
Fsp4HI GCNGC 6 cut(s) 702, 820, 935, 950, 1500, 1820
FspBI CTAG 6 cut(s) 234, 479, 537, 1026, 1848, 1853
GluI GCNGC 6 cut(s) 702, 820, 935, 950, 1500, 1820
GsaI CCCAGC 2 cut(s) 1234, 1635
GsuI CTGGAG 3 cut(s) 165, 1392, 1728
HaeIII GGCC 6 cut(s) 194, 226, 822, 1507, 1857, 1882
HapII CCGG 3 cut(s) 681, 1566, 1625
HincII GTYRAC 1 cut(s) 1591
HindII GTYRAC 1 cut(s) 1591
HindIII AAGCTT 2 cut(s) 59, 457
HpaI GTTAAC 1 cut(s) 1591
HpaII CCGG 3 cut(s) 681, 1566, 1625
HphI GGTGA 8 cut(s) 29, 526, 533, 602, 611, 626, 669, 1919
Hpy166II GTNNAC 3 cut(s) 583, 1478, 1591
Hpy188III TCNNGA 7 cut(s) 144, 479, 914, 1409, 1890, 1971, 1983
Hpy8I GTNNAC 3 cut(s) 583, 1478, 1591
HpyAV CCTTC 7 cut(s) 31, 87, 387, 443, 511, 1011, 1317
HpyCH4III ACNGT 9 cut(s) 161, 248, 253, 545, 587, 596, 756, 1464, 1588
HpyF10VI GCNNNNNNNGC 3 cut(s) 698, 1628, 1816
HpyF3I CTNAG 8 cut(s) 510, 1252, 1363, 1385, 1418, 1530, 1859, 1869
KspAI GTTAAC 1 cut(s) 1591
Kzo9I GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 302, 1031, 1433, 1553, 1628
Lsp1109I GCAGC 5 cut(s) 713, 936, 946, 1486, 1831
LweI GCATC 3 cut(s) 564, 757, 1159
MaeI CTAG 6 cut(s) 234, 479, 537, 1026, 1848, 1853
MaeIII GTNAC 5 cut(s) 208, 590, 859, 962, 1759
MalI GATC 8 cut(s) 315, 468, 673, 906, 1361, 1383, 1637, 1969
MboI GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
MfeI CAATTG 1 cut(s) 1728
MflI RGATCY 3 cut(s) 671, 1359, 1381
MhlI GDGCHC 2 cut(s) 96, 1438
MlyI GAGTC 6 cut(s) 343, 776, 860, 1354, 1556, 1719
MmeI TCCRAC 1 cut(s) 724
Mph1103I ATGCAT 2 cut(s) 156, 772
MseI TTAA 9 cut(s) 177, 326, 1001, 1041, 1133, 1329, 1337, 1590, 1679
MslI CAYNNNNRTG 4 cut(s) 159, 407, 1049, 1932
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 3 cut(s) 681, 1566, 1625
MspR9I CCNGG 8 cut(s) 31, 196, 228, 681, 779, 1626, 1707, 1842
MunI CAATTG 1 cut(s) 1728
Mva1269I GAATGC 2 cut(s) 1213, 1234
MvaI CCWGG 6 cut(s) 31, 196, 228, 779, 1707, 1842
MwoI GCNNNNNNNGC 3 cut(s) 698, 1628, 1816
NciI CCSGG 2 cut(s) 681, 1626
NcoI CCATGG 1 cut(s) 1537
NdeII GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
NlaIV GGNNCC 4 cut(s) 298, 776, 803, 1704
NmeAIII GCCGAG 1 cut(s) 1858
NmuCI GTSAC 2 cut(s) 590, 1759
NsiI ATGCAT 2 cut(s) 156, 772
NspI RCATGY 2 cut(s) 476, 1489
OliI CACNNNNGTG 2 cut(s) 407, 1049
PaeI GCATGC 1 cut(s) 1489
PceI AGGCCT 1 cut(s) 1857
PciSI GCTCTTC 1 cut(s) 1203
PctI GAATGC 2 cut(s) 1213, 1234
PfeI GAWTC 5 cut(s) 69, 1072, 1109, 1196, 1295
PflFI GACNNNGTC 1 cut(s) 649
PflMI CCANNNNNTGG 3 cut(s) 345, 362, 498
PfoI TCCNGGA 1 cut(s) 777
PkrI GCNGC 6 cut(s) 703, 821, 936, 951, 1501, 1821
PleI GAGTC 6 cut(s) 343, 776, 860, 1353, 1556, 1718
PpsI GAGTC 6 cut(s) 343, 776, 860, 1353, 1556, 1718
PpuMI RGGWCCY 2 cut(s) 775, 1703
Psp5II RGGWCCY 2 cut(s) 775, 1703
Psp6I CCWGG 6 cut(s) 29, 194, 226, 777, 1705, 1840
PspEI GGTNACC 1 cut(s) 590
PspFI CCCAGC 2 cut(s) 1230, 1631
PspGI CCWGG 6 cut(s) 29, 194, 226, 777, 1705, 1840
PspN4I GGNNCC 4 cut(s) 298, 776, 803, 1704
PspPI GGNCC 7 cut(s) 224, 230, 775, 801, 1505, 1534, 1703
PspPPI RGGWCCY 2 cut(s) 775, 1703
PstI CTGCAG 1 cut(s) 1504
PsuI RGATCY 3 cut(s) 671, 1359, 1381
PsyI GACNNNGTC 1 cut(s) 649
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 6 cut(s) 250, 289, 584, 758, 790, 1171
RsaNI GTAC 6 cut(s) 249, 288, 583, 757, 789, 1170
RseI CAYNNNNRTG 4 cut(s) 159, 407, 1049, 1932
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 9 cut(s) 177, 326, 1001, 1041, 1133, 1329, 1337, 1590, 1679
SatI GCNGC 6 cut(s) 702, 820, 935, 950, 1500, 1820
Sau3AI GATC 8 cut(s) 313, 466, 671, 904, 1359, 1381, 1635, 1967
Sau96I GGNCC 7 cut(s) 224, 230, 775, 801, 1505, 1534, 1703
ScaI AGTACT 1 cut(s) 790
SchI GAGTC 6 cut(s) 343, 776, 860, 1354, 1556, 1719
ScrFI CCNGG 8 cut(s) 31, 196, 228, 681, 779, 1626, 1707, 1842
SduI GDGCHC 2 cut(s) 96, 1438
SfaNI GCATC 3 cut(s) 564, 757, 1159
SfcI CTRYAG 2 cut(s) 1077, 1500
SinI GGWCC 5 cut(s) 230, 775, 801, 1534, 1703
SmiMI CAYNNNNRTG 4 cut(s) 159, 407, 1049, 1932
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SphI GCATGC 1 cut(s) 1489
SseBI AGGCCT 1 cut(s) 1857
SsiI CCGC 2 cut(s) 366, 819
SspMI CTAG 6 cut(s) 234, 479, 537, 1026, 1848, 1853
StuI AGGCCT 1 cut(s) 1857
StyD4I CCNGG 8 cut(s) 29, 194, 226, 679, 777, 1624, 1705, 1840
StyI CCWWGG 2 cut(s) 233, 1537
TaaI ACNGT 9 cut(s) 161, 248, 253, 545, 587, 596, 756, 1464, 1588
TaqI TCGA 3 cut(s) 352, 810, 915
TatI WGTACW 1 cut(s) 788
TauI GCSGC 1 cut(s) 822
TfiI GAWTC 5 cut(s) 69, 1072, 1109, 1196, 1295
Tru1I TTAA 9 cut(s) 177, 326, 1001, 1041, 1133, 1329, 1337, 1590, 1679
Tru9I TTAA 9 cut(s) 177, 326, 1001, 1041, 1133, 1329, 1337, 1590, 1679
TscAI CASTG 2 cut(s) 166, 856
TseFI GTSAC 2 cut(s) 590, 1759
TseI GCWGC 5 cut(s) 701, 934, 949, 1499, 1819
Tsp45I GTSAC 2 cut(s) 590, 1759
TspGWI ACGGA 1 cut(s) 1608
TspRI CASTG 2 cut(s) 166, 856
Tth111I GACNNNGTC 1 cut(s) 649
Van91I CCANNNNNTGG 3 cut(s) 345, 362, 498
VpaK11BI GGWCC 5 cut(s) 230, 775, 801, 1534, 1703
XagI CCTNNNNNAGG 1 cut(s) 1311
XapI RAATTY 6 cut(s) 984, 1037, 1368, 1389, 1666, 1831
XbaI TCTAGA 1 cut(s) 478
XceI RCATGY 2 cut(s) 476, 1489
XmaJI CCTAGG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 1477
XspI CTAG 6 cut(s) 234, 479, 537, 1026, 1848, 1853
ZrmI AGTACT 1 cut(s) 790
Zsp2I ATGCAT 2 cut(s) 156, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.