pycom12g19310

Kelch motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008868
Physical Location & Seq
Forward (+)
21199025 .. 21200643
1619 bp
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UTR
Exon/CDS
Intron
pycom12g19310.1

Sequence Viewer

Length: 477 bp
ATGGGGTCTTTGGGAGGTGAAGCTGCAAGGAAGAAGGCAATGTGGCTCTATCCAAAGATCATGGGATTTAATCCTTCTGAAAGATGGGGGCACTCTGCCTGCTATTCTCGTGGGGTTTTGTATATTTTTGGGGGATGTTGTGGCGGTATGCATTTCAGCGATGTTCTTGTGCTTGATCTTGAGAAAATGGTATGGAACACTCTTGTGTCAACCGGTCAAGGGCCTGGTCCTAGAGACAGCCATAGTGCCATTGTTCTAGGGCACAAGATGATAGTTTTCGGGGGAACTAATGGCTCCAAGAAGGTAAATGATCTTCACATATTGAATCTTGTGACCAAAGAATGGACACGGCCAGAATGTACGGGTACTCCACCTTCACCCCGCGAAAGTCACACTGCTACCCTTGTTGGTGATGAAAAATTAGTGATATTCGGGGGTAGTGGAGAAGGTAAAGGAAATTACTCCGTCGGTGAATGA

Protein Analysis

159

Amino Acids

16.95

Weight (kDa)

8.36

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_HCF PF13854 10 - 95 2.5e-14 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 12 - 151 7.2e-37 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 13 - 149 4.6e-43 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1 PF01344 30 - 68 1e-06 Kelch motif
Kelch_FKB95 PF25210 32 - 146 1.2e-07 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 51 - 148 1e-16 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 77 - 117 5.8e-08 Kelch motif
Kelch_HCF PF13854 88 - 146 2.2e-09 Host cell factor, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 342
AccII CGCG 1 cut(s) 384
AciI CCGC 2 cut(s) 144, 382
AcoI YGGCCR 1 cut(s) 350
AfaI GTAC 2 cut(s) 361, 367
AfiI CCNNNNNNNGG 2 cut(s) 219, 342
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 1 cut(s) 223
AleI CACNNNNGTG 1 cut(s) 203
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 228
AoxI GGCC 2 cut(s) 221, 350
ApeKI GCWGC 1 cut(s) 23
AsiGI ACCGGT 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 221, 227
AsuHPI GGTGA 3 cut(s) 29, 369, 422
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 93, 264
BarI GAAGNNNNNNTAC 4 cut(s) 297, 329, 358, 390
BauI CACGAG 1 cut(s) 108
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 2 cut(s) 231, 257
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 221, 227
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 352, 382
Bsc4I CCNNNNNNNGG 2 cut(s) 219, 342
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 225
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 219, 342
BseMI GCAATG 1 cut(s) 45
BseSI GKGCMC 2 cut(s) 93, 264
BseXI GCAGC 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 223, 352
BshTI ACCGGT 1 cut(s) 212
BsiSI CCGG 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 219, 342
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 2 cut(s) 223, 352
Bsp1286I GDGCHC 2 cut(s) 93, 264
Bsp143I GATC 3 cut(s) 57, 175, 310
BspACI CCGC 2 cut(s) 144, 382
BspANI GGCC 2 cut(s) 223, 352
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 295
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssMI GATC 3 cut(s) 57, 175, 310
BssSI CACGAG 1 cut(s) 108
Bst2BI CACGAG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 100
BstF5I GGATG 1 cut(s) 140
BstFNI CGCG 1 cut(s) 384
BstKTI GATC 3 cut(s) 60, 178, 313
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 3 cut(s) 57, 175, 310
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstSLI GKGCMC 2 cut(s) 93, 264
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 10
BsuRI GGCC 2 cut(s) 223, 352
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 1 cut(s) 140
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 221, 227
Csp6I GTAC 2 cut(s) 360, 366
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 1 cut(s) 61
CviJI RGCY 6 cut(s) 23, 46, 223, 240, 294, 352
CviKI_1 RGCY 6 cut(s) 23, 46, 223, 240, 294, 352
CviQI GTAC 2 cut(s) 360, 366
DpnI GATC 3 cut(s) 59, 177, 312
DpnII GATC 3 cut(s) 57, 175, 310
EaeI YGGCCR 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 153
FaeI CATG 1 cut(s) 64
FaiI YATR 6 cut(s) 62, 123, 149, 193, 243, 320
FatI CATG 1 cut(s) 60
FauI CCCGC 1 cut(s) 389
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 2 cut(s) 231, 257
GluI GCNGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 223, 352
HapII CCGG 1 cut(s) 213
Hin1II CATG 1 cut(s) 64
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 1 cut(s) 325
HpaII CCGG 1 cut(s) 213
HphI GGTGA 3 cut(s) 29, 369, 422
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 5 cut(s) 28, 84, 295, 384, 440
HpyCH4V TGCA 2 cut(s) 26, 151
Hsp92II CATG 1 cut(s) 64
Kzo9I GATC 3 cut(s) 57, 175, 310
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 5 cut(s) 112, 210, 226, 237, 366
Lsp1109I GCAGC 1 cut(s) 10
MaeI CTAG 2 cut(s) 231, 257
MaeIII GTNAC 2 cut(s) 331, 389
MalI GATC 3 cut(s) 59, 177, 312
MboI GATC 3 cut(s) 57, 175, 310
MboII GAAGA 2 cut(s) 43, 305
MhlI GDGCHC 2 cut(s) 93, 264
MluCI AATT 2 cut(s) 419, 457
MnlI CCTC 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 153
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 1 cut(s) 384
NdeII GATC 3 cut(s) 57, 175, 310
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 2 cut(s) 331, 389
NsiI ATGCAT 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 203
PfeI GAWTC 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 342
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 2 cut(s) 221, 227
RsaI GTAC 2 cut(s) 361, 367
RsaNI GTAC 2 cut(s) 360, 366
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 3 cut(s) 57, 175, 310
Sau96I GGNCC 2 cut(s) 221, 227
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 2 cut(s) 93, 264
SetI ASST 5 cut(s) 19, 25, 306, 376, 451
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 2 cut(s) 419, 457
SsiI CCGC 2 cut(s) 144, 382
SspMI CTAG 2 cut(s) 231, 257
StyD4I CCNGG 1 cut(s) 223
TasI AATT 2 cut(s) 419, 457
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 2 cut(s) 331, 389
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 2 cut(s) 331, 389
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 454
TspRI CASTG 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 227
XspI CTAG 2 cut(s) 231, 257
Zsp2I ATGCAT 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.