Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008868

Physical Location & Seq

Genomic Coordinates Forward (+)

28586391 .. 28590534

Gene Structure 4144 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1205300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGGGGTCTTTGGGAGGTGAAGCTGCAAGGAAGAAAGCAATGTGGCTCTATCCAAAGATCATGGGATTTAATCCTTCTGAAAGATGGGGGCACTCTGCCTGCTATTCTCGTGGGGTTTTGTATATTTTTGGGGGATGTTGCGGCGGTATGCATTTCAGCGATGTTCTTGTGCTTGATCTTGAGAAAATGTTATGGAACACTCTTGTGTCAACCGGTCAAGGGCCTGGTCCTAGAGACAGCCATAGTGCCATTGTTCTAGGGCACAAGATGATAGTTTTCGGGGGAACTAATGGCTCCAAGAAGGTAAATGATCTTCACATATTGAATCTTGTGACCAAAGAATGGACACGGCCAGAATGTACGGGTACTCCACCTTCACCCCGAGAAAGTCATACTGCTACCCTTGTTGGTGATGAAAAATTAGTGATATTCGGGGGTAGTGGAGAAGGTAAAGGAAATTACTTGAATGATCTTCATGTTCTAGACCTTAATACCATGAGATGGACTTCTCCTGAGGTGACGGGTGATATTCCTGTTCCTCGAGACAGTCATAGCACAATTGTGATGGGGAAGAAGCTTCTTGTGTATGGTGGGGACCGTGGTGATAGGTATTATGGTGGTGTAGATATGCTTGACATGAACACACTGACATGGTCGAGGTTGGCTGTTCAAGGATCTTCACCGGGTGCCAGGGCAGGTCATGCAACTGTTAGTATCGGGACAAAGGTTTATGTCATTGGAGGGGTTGGAGACAAACATTATTACAACGATGTTTGGGTCCTAGATGTGATTACTTGTTCATGGACTCAACTTGAAATATGTGGCCAACATCCACAAGGACGATTCTCTCATACTGCTGTTGTTACAGATTTTGATATTGCCGTCTACGGAGGGTGTGGTGAGGACGAGCGCCCTCTGAATGAGTTGTTGGTGTTGCATCTTGGAGCAGAACATCTCAATGGTCGTTACAATGTTTCCATGTGCAAAATTTTTGGAAGCCACTGGAACCGAGAGAGGGGGAGGATCTCAAAGGGAGCAGATTTTAACACGCAGAAACCTATGCTAATGGGGAATCATGTAGTAGTACGAGAGACAACCCACGGACCAGAATCAGAAGCAAGACGGTCTCTTCAGTTCAAATCAGATACTTTACATCCAAAGAGGAGAAGAACCACAAGTACAAAATCAATAGATCTCGAATCAGAACAAGAGGAGCATTCTCTATCACTCTCCCAGCATTCATCACCATCACACTCGGATCAAGAGCAGATCCCGAATCCTAATATTGTTGATTCCAACCAAGGTTCTCAAGGTTTTCATTTGTTTAAGAAACTCAATCATAGTCCGAGCAATGGCCAATCTCCCATTGTCACGAGCAGCCATAAAGCTAATAGAAATTCTGGACAAAGATCTCCAGACCTTAACTTGTTAGGAGAGCGTCACACCGAACCAAAACGAGAACAGTATCATCATGTCAACATTGGTAGACCAAGTATGCAATTTCAAGCAGTAGAACAAAAACACTTGGAGGCAGGGCCTATTCAGAACATGATGGGTGCTGAGGTTCAAGGCAAAGTTGATGGAGCATTCGACTCAGGTTTCCTAATGACCGCGATGGTTAATGGAAAGATATATAGAGGAGTCTTATTTGCACCAGGACCCCAGATCATCTCAAGAAGGCCAACTTTCGTGCAAAATCCTTCGTCTTCAACAAGCCAAATTCCCATTTCTGAAGCAAAACCATTTCCAAACTCGAATCATCACGAGCCACCCTTGAAGCTATCTGAGCAACCAATGAGGAATTCCATGCCGGAGTCTGGCCTGGGCCTCAGCCAACCTCAGGTTGCTCGACCATTTTCGGTCATTCGAGCCAATCCATCTGTAGCCAAAGAAAGAAGTGATCTTCCTGGTGTGTTTTTAACACTTGGGGGACCAGGAAGTCCAACTGGTGGATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_3 Kelch_4

Protein Analysis

653

Amino Acids

71.32

Weight (kDa)

8.48

Isoelectric Point (pI)

41.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kelch_HCF	PF13854	11 - 96	8.2e-13	Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	13 - 198	1.2e-59	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42	PF13415	13 - 149	3.4e-35	FBX42, beta-propeller domain
Kelch_1	PF01344	30 - 68	9.5e-06	Kelch motif
Kelch_FKB95	PF25210	36 - 145	7.6e-06	FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1	PF24981	52 - 198	5.3e-23	Attractin/LZTR1 beta-propeller
Kelch_1	PF01344	77 - 117	4.6e-07	Kelch motif
Kelch_HCF	PF13854	100 - 198	6.4e-18	Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	130 - 314	9.1e-52	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_2	PF07646	131 - 171	3.3e-06	Kelch motif
Beta-prop_FBX42	PF13415	143 - 315	2.8e-38	FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1	PF24981	155 - 301	8.5e-22	Attractin/LZTR1 beta-propeller
Kelch_HCF	PF13854	191 - 248	2.5e-08	Host cell factor, Kelch-repeats domain
Kelch_FKB95	PF25210	226 - 304	1.1e-06	FKB95, Kelch-repeats domain
Kelch_1	PF01344	230 - 271	4e-10	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008868)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g07501 FvH4_6g07501
malus_domestica	MD00G1208300.v1.1 MD12G1205300.v1.1
prunus_persica	Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1 Prupe.6G312400_v2.0.a1
pyrus_communis	pycom04g17040 pycom12g19310 pycom12g19320
rosa_chinensis	RchiOBHm_Chr3g0457201
rosa_laevigata	RLG00000025243
rosa_multiflora	Rmu_co8495289.1_g000002 Rmu_sc0000488.1_g000018 Rmu_sc0016952.1_g000006
rosa_roxburghii	Rroxscaffold_6G00422120
rosa_rugosa	Rorug03G0019400
rosa_samantha	Rh3AG079200 Rh3BG080900 Rh3CG081100 Rh3DG082900
rosa_wichuraiana	Rw3G006820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1938
Acc36I	ACCTGC	1 cut(s)	686
AccB1I	GGYRCC	1 cut(s)	686
AccB7I	CCANNNNNTGG	3 cut(s)	342, 501, 1949
AccI	GTMKAC	2 cut(s)	885, 1486
AccII	CGCG	1 cut(s)	1613
AciI	CCGC	3 cut(s)	141, 144, 1611
AclWI	GGATC	4 cut(s)	682, 1031, 1264, 1266
AcoI	YGGCCR	3 cut(s)	350, 823, 1354
AcsI	RAATTY	4 cut(s)	987, 1396, 1719, 1801
AcuI	CTGAAG	2 cut(s)	1115, 1752
AdeI	CACNNNGTG	1 cut(s)	686
AfaI	GTAC	4 cut(s)	361, 367, 1086, 1180
AfiI	CCNNNNNNNGG	8 cut(s)	219, 342, 501, 1015, 1352, 1817, 1840, 1949
AgeI	ACCGGT	1 cut(s)	212
AgsI	TTSAA	9 cut(s)	325, 466, 671, 815, 1138, 1505, 1568, 1710, 1777
AjnI	CCWGG	6 cut(s)	223, 689, 1654, 1821, 1906, 1933
AjuI	GAANNNNNNNTTGG	4 cut(s)	911, 943, 1786, 1818
AleI	CACNNNNGTG	2 cut(s)	203, 560
AluBI	AGCT	4 cut(s)	23, 577, 1388, 1780
AluI	AGCT	4 cut(s)	23, 577, 1388, 1780
Alw26I	GTCTC	5 cut(s)	228, 537, 744, 1085, 1131
AlwI	GGATC	4 cut(s)	682, 1031, 1264, 1266
Ama87I	CYCGRG	2 cut(s)	381, 540
AoxI	GGCC	8 cut(s)	221, 350, 823, 1354, 1535, 1679, 1819, 1825
ApeKI	GCWGC	2 cut(s)	23, 1377
ApoI	RAATTY	4 cut(s)	987, 1396, 1719, 1801
AsiGI	ACCGGT	1 cut(s)	212
AspLEI	GCGC	1 cut(s)	912
AspS9I	GGNCC	9 cut(s)	221, 227, 595, 778, 1103, 1535, 1658, 1825, 1931
AsuC2I	CCSGG	1 cut(s)	684
AsuHPI	GGTGA	9 cut(s)	29, 369, 422, 529, 536, 614, 672, 911, 1236
AvaI	CYCGRG	2 cut(s)	381, 540
AvaII	GGWCC	6 cut(s)	227, 595, 778, 1103, 1658, 1931
AxyI	CCTNAGG	2 cut(s)	513, 1839
BaeGI	GKGCMC	2 cut(s)	93, 264
BalI	TGGCCA	2 cut(s)	825, 1356
BanI	GGYRCC	1 cut(s)	686
BarI	GAAGNNNNNNTAC	4 cut(s)	297, 329, 358, 390
BauI	CACGAG	3 cut(s)	108, 1372, 1763
BbsI	GAAGAC	1 cut(s)	1698
BbvCI	CCTCAGC	2 cut(s)	1560, 1829
BbvI	GCAGC	2 cut(s)	10, 1389
BccI	CCATC	8 cut(s)	78, 495, 559, 1255, 1546, 1574, 1609, 1885
BceAI	ACGGC	2 cut(s)	365, 866
BciT130I	CCWGG	6 cut(s)	225, 691, 1656, 1823, 1908, 1935
BcnI	CCSGG	1 cut(s)	684
BcoDI	GTCTC	5 cut(s)	228, 537, 744, 1085, 1131
BfaI	CTAG	4 cut(s)	231, 257, 482, 782
BfmI	CTRYAG	1 cut(s)	1881
BfoI	RGCGCY	1 cut(s)	913
BfuAI	ACCTGC	1 cut(s)	686
BglII	AGATCT	2 cut(s)	1192, 1409
BisI	GCNGC	3 cut(s)	24, 142, 1378
BlsI	GCNGC	3 cut(s)	25, 143, 1379
Bme1390I	CCNGG	7 cut(s)	225, 684, 691, 1656, 1823, 1908, 1935
Bme18I	GGWCC	6 cut(s)	227, 595, 778, 1103, 1658, 1931
BmeT110I	CYCGRG	2 cut(s)	381, 540
BmgT120I	GGNCC	9 cut(s)	221, 227, 595, 778, 1103, 1535, 1658, 1825, 1931
BmiI	GGNNCC	7 cut(s)	295, 596, 688, 779, 1007, 1660, 1932
BmrFI	CCNGG	7 cut(s)	225, 684, 691, 1656, 1823, 1908, 1935
BmsI	GCATC	1 cut(s)	946
BpiI	GAAGAC	1 cut(s)	1698
BpmI	CTGGAG	1 cut(s)	1398
Bpu10I	CCTNAGC	2 cut(s)	1560, 1829
BpuEI	CTTGAG	3 cut(s)	200, 1293, 1657
BpuMI	CCSGG	1 cut(s)	684
BsaI	GGTCTC	1 cut(s)	1131
BsaJI	CCNNGG	5 cut(s)	598, 690, 1099, 1300, 1822
BsaWI	WCCGGW	1 cut(s)	212
BsaXI	ACNNNNNCTCC	2 cut(s)	352, 382
Bsc4I	CCNNNNNNNGG	8 cut(s)	219, 342, 501, 1015, 1352, 1817, 1840, 1949
Bse118I	RCCGGY	1 cut(s)	212
Bse1I	ACTGG	2 cut(s)	1007, 1951
Bse21I	CCTNAGG	2 cut(s)	513, 1839
Bse3DI	GCAATG	2 cut(s)	45, 1357
BseBI	CCWGG	6 cut(s)	225, 691, 1656, 1823, 1908, 1935
BseDI	CCNNGG	5 cut(s)	598, 690, 1099, 1300, 1822
BseGI	GGATG	3 cut(s)	140, 829, 1153
BseLI	CCNNNNNNNGG	8 cut(s)	219, 342, 501, 1015, 1352, 1817, 1840, 1949
BseMI	GCAATG	2 cut(s)	45, 1357
BseMII	CTCAG	6 cut(s)	504, 1551, 1608, 1776, 1843, 1853
BseNI	ACTGG	2 cut(s)	1007, 1951
BseRI	GAGGAG	3 cut(s)	1177, 1226, 1653
BseSI	GKGCMC	2 cut(s)	93, 264
BseXI	GCAGC	2 cut(s)	10, 1389
BseYI	CCCAGC	1 cut(s)	1233
Bsh1236I	CGCG	1 cut(s)	1613
BshFI	GGCC	8 cut(s)	223, 352, 825, 1356, 1537, 1681, 1821, 1827
BshNI	GGYRCC	1 cut(s)	686
BshTI	ACCGGT	1 cut(s)	212
BsiHKCI	CYCGRG	2 cut(s)	381, 540
BsiSI	CCGG	3 cut(s)	213, 683, 1811
BslFI	GGGAC	3 cut(s)	608, 733, 1944
BslI	CCNNNNNNNGG	8 cut(s)	219, 342, 501, 1015, 1352, 1817, 1840, 1949
BsmAI	GTCTC	5 cut(s)	228, 537, 744, 1085, 1131
BsmFI	GGGAC	3 cut(s)	608, 733, 1944
BsmI	GAATGC	3 cut(s)	1216, 1237, 1586
BsnI	GGCC	8 cut(s)	223, 352, 825, 1356, 1537, 1681, 1821, 1827
Bso31I	GGTCTC	1 cut(s)	1131
BsoBI	CYCGRG	2 cut(s)	381, 540
Bsp1286I	GDGCHC	2 cut(s)	93, 264
BspACI	CCGC	3 cut(s)	141, 144, 1611
BspANI	GGCC	8 cut(s)	223, 352, 825, 1356, 1537, 1681, 1821, 1827
BspCNI	CTCAG	6 cut(s)	505, 1552, 1607, 1777, 1842, 1852
BspFNI	CGCG	1 cut(s)	1613
BspLI	GGNNCC	7 cut(s)	295, 596, 688, 779, 1007, 1660, 1932
BspMI	ACCTGC	1 cut(s)	686
BspPI	GGATC	4 cut(s)	682, 1031, 1264, 1266
BspT107I	GGYRCC	1 cut(s)	686
BspTNI	GGTCTC	1 cut(s)	1131
BsrDI	GCAATG	2 cut(s)	45, 1357
BsrFI	RCCGGY	1 cut(s)	212
BsrI	ACTGG	2 cut(s)	1007, 1951
BssAI	RCCGGY	1 cut(s)	212
BssECI	CCNNGG	5 cut(s)	598, 690, 1099, 1300, 1822
BssSI	CACGAG	3 cut(s)	108, 1372, 1763
BssT1I	CCWWGG	1 cut(s)	1300
Bst2BI	CACGAG	3 cut(s)	108, 1372, 1763
Bst2UI	CCWGG	6 cut(s)	225, 691, 1656, 1823, 1908, 1935
Bst4CI	ACNGT	5 cut(s)	548, 599, 709, 1125, 1464
Bst6I	CTCTTC	1 cut(s)	1134
BstAPI	GCANNNNNTGC	1 cut(s)	701
BstC8I	GCNNGC	1 cut(s)	100
BstDEI	CTNAG	7 cut(s)	513, 1560, 1594, 1785, 1829, 1839, 1956
BstDSI	CCRYGG	2 cut(s)	598, 1099
BstF5I	GGATG	3 cut(s)	140, 829, 1153
BstFNI	CGCG	1 cut(s)	1613
BstH2I	RGCGCY	1 cut(s)	913
BstHHI	GCGC	1 cut(s)	912
BstMAI	GTCTC	5 cut(s)	228, 537, 744, 1085, 1131
BstMWI	GCNNNNNNNGC	2 cut(s)	701, 1786
BstNI	CCWGG	6 cut(s)	225, 691, 1656, 1823, 1908, 1935
BstSCI	CCNGG	7 cut(s)	223, 682, 689, 1654, 1821, 1906, 1933
BstSFI	CTRYAG	1 cut(s)	1881
BstSLI	GKGCMC	2 cut(s)	93, 264
BstUI	CGCG	1 cut(s)	1613
BstV1I	GCAGC	2 cut(s)	10, 1389
BstV2I	GAAGAC	1 cut(s)	1698
BstX2I	RGATCY	6 cut(s)	674, 1023, 1192, 1269, 1409, 1952
BstYI	RGATCY	6 cut(s)	674, 1023, 1192, 1269, 1409, 1952
Bsu36I	CCTNAGG	2 cut(s)	513, 1839
BsuRI	GGCC	8 cut(s)	223, 352, 825, 1356, 1537, 1681, 1821, 1827
BtgI	CCRYGG	2 cut(s)	598, 1099
BtgZI	GCGATG	2 cut(s)	174, 1628
BtsCI	GGATG	3 cut(s)	140, 829, 1153
BtsIMutI	CAGTG	2 cut(s)	644, 1000
BveI	ACCTGC	1 cut(s)	686
Cac8I	GCNNGC	1 cut(s)	100
CfoI	GCGC	1 cut(s)	912
Cfr10I	RCCGGY	1 cut(s)	212
Cfr13I	GGNCC	9 cut(s)	221, 227, 595, 778, 1103, 1535, 1658, 1825, 1931
CseI	GACGC	1 cut(s)	1427
Csp6I	GTAC	4 cut(s)	360, 366, 1085, 1179
CspAI	ACCGGT	1 cut(s)	212
CviQI	GTAC	4 cut(s)	360, 366, 1085, 1179
DdeI	CTNAG	7 cut(s)	513, 1560, 1594, 1785, 1829, 1839, 1956
DraIII	CACNNNGTG	1 cut(s)	686
DrdI	GACNNNNNNGTC	1 cut(s)	1938
DseDI	GACNNNNNNGTC	1 cut(s)	1938
EaeI	YGGCCR	3 cut(s)	350, 823, 1354
Eam1104I	CTCTTC	1 cut(s)	1134
EarI	CTCTTC	1 cut(s)	1134
Eco130I	CCWWGG	1 cut(s)	1300
Eco31I	GGTCTC	1 cut(s)	1131
Eco47I	GGWCC	6 cut(s)	227, 595, 778, 1103, 1658, 1931
Eco57I	CTGAAG	2 cut(s)	1115, 1752
Eco81I	CCTNAGG	2 cut(s)	513, 1839
Eco88I	CYCGRG	2 cut(s)	381, 540
EcoO109I	RGGNCCY	4 cut(s)	221, 778, 1535, 1658
EcoRI	GAATTC	1 cut(s)	1801
EcoRII	CCWGG	6 cut(s)	223, 689, 1654, 1821, 1906, 1933
EcoT14I	CCWWGG	1 cut(s)	1300
EcoT22I	ATGCAT	1 cut(s)	153
ErhI	CCWWGG	1 cut(s)	1300
FalI	AAGNNNNNCTT	2 cut(s)	1669, 1701
FaqI	GGGAC	3 cut(s)	608, 733, 1944
FblI	GTMKAC	2 cut(s)	885, 1486
Fnu4HI	GCNGC	3 cut(s)	24, 142, 1378
FokI	GGATG	3 cut(s)	147, 816, 1140
Fsp4HI	GCNGC	3 cut(s)	24, 142, 1378
FspBI	CTAG	4 cut(s)	231, 257, 482, 782
GlaI	GCGC	1 cut(s)	911
GluI	GCNGC	3 cut(s)	24, 142, 1378
GsaI	CCCAGC	1 cut(s)	1237
GsuI	CTGGAG	1 cut(s)	1398
HaeII	RGCGCY	1 cut(s)	913
HaeIII	GGCC	8 cut(s)	223, 352, 825, 1356, 1537, 1681, 1821, 1827
HapII	CCGG	3 cut(s)	213, 683, 1811
HgaI	GACGC	1 cut(s)	1427
HhaI	GCGC	1 cut(s)	912
Hin6I	GCGC	1 cut(s)	910
HinP1I	GCGC	1 cut(s)	910
HincII	GTYRAC	2 cut(s)	210, 1477
HindII	GTYRAC	2 cut(s)	210, 1477
HindIII	AAGCTT	1 cut(s)	575
HpaII	CCGG	3 cut(s)	213, 683, 1811
HphI	GGTGA	9 cut(s)	29, 369, 422, 529, 536, 614, 672, 911, 1236
Hpy166II	GTNNAC	4 cut(s)	210, 886, 1477, 1487
Hpy8I	GTNNAC	4 cut(s)	210, 886, 1477, 1487
HpyAV	CCTTC	6 cut(s)	84, 295, 384, 440, 1671, 1710
HpyCH4III	ACNGT	5 cut(s)	548, 599, 709, 1125, 1464
HpyCH4V	TGCA	8 cut(s)	26, 151, 704, 937, 984, 1498, 1652, 1693
HpyF10VI	GCNNNNNNNGC	2 cut(s)	701, 1786
HpyF3I	CTNAG	7 cut(s)	513, 1560, 1594, 1785, 1829, 1839, 1956
HspAI	GCGC	1 cut(s)	910
LmnI	GCTCC	5 cut(s)	299, 944, 1034, 1213, 1583
Lsp1109I	GCAGC	2 cut(s)	10, 1389
LweI	GCATC	1 cut(s)	946
MaeI	CTAG	4 cut(s)	231, 257, 482, 782
MaeIII	GTNAC	6 cut(s)	331, 517, 862, 965, 1369, 1439
MboII	GAAGA	9 cut(s)	43, 305, 464, 583, 669, 1121, 1179, 1698, 1895
MfeI	CAATTG	1 cut(s)	558
MflI	RGATCY	6 cut(s)	674, 1023, 1192, 1269, 1409, 1952
MhlI	GDGCHC	2 cut(s)	93, 264
MlsI	TGGCCA	2 cut(s)	825, 1356
MluCI	AATT	8 cut(s)	419, 457, 558, 987, 1396, 1499, 1719, 1801
MluNI	TGGCCA	2 cut(s)	825, 1356
MlyI	GAGTC	4 cut(s)	799, 1586, 1650, 1823
MmeI	TCCRAC	2 cut(s)	727, 1320
Mox20I	TGGCCA	2 cut(s)	825, 1356
Mph1103I	ATGCAT	1 cut(s)	153
MscI	TGGCCA	2 cut(s)	825, 1356
MseI	TTAA	7 cut(s)	69, 489, 1044, 1326, 1422, 1620, 1919
MslI	CAYNNNNRTG	4 cut(s)	203, 560, 649, 957
Msp20I	TGGCCA	2 cut(s)	825, 1356
MspI	CCGG	3 cut(s)	213, 683, 1811
MspR9I	CCNGG	7 cut(s)	225, 684, 691, 1656, 1823, 1908, 1935
MunI	CAATTG	1 cut(s)	558
Mva1269I	GAATGC	3 cut(s)	1216, 1237, 1586
MvaI	CCWGG	6 cut(s)	225, 691, 1656, 1823, 1908, 1935
MvnI	CGCG	1 cut(s)	1613
MwoI	GCNNNNNNNGC	2 cut(s)	701, 1786
NciI	CCSGG	1 cut(s)	684
NlaIV	GGNNCC	7 cut(s)	295, 596, 688, 779, 1007, 1660, 1932
NmuCI	GTSAC	4 cut(s)	331, 517, 1369, 1439
NsiI	ATGCAT	1 cut(s)	153
OliI	CACNNNNGTG	2 cut(s)	203, 560
PaeR7I	CTCGAG	1 cut(s)	540
PctI	GAATGC	3 cut(s)	1216, 1237, 1586
PfeI	GAWTC	8 cut(s)	325, 843, 1072, 1109, 1199, 1276, 1292, 1756
PflFI	GACNNNGTC	1 cut(s)	652
PflMI	CCANNNNNTGG	3 cut(s)	342, 501, 1949
PinAI	ACCGGT	1 cut(s)	212
PkrI	GCNGC	3 cut(s)	25, 143, 1379
PleI	GAGTC	4 cut(s)	799, 1586, 1649, 1822
PpsI	GAGTC	4 cut(s)	799, 1586, 1649, 1822
PpuMI	RGGWCCY	2 cut(s)	778, 1658
Psp5II	RGGWCCY	2 cut(s)	778, 1658
Psp6I	CCWGG	6 cut(s)	223, 689, 1654, 1821, 1906, 1933
PspFI	CCCAGC	1 cut(s)	1233
PspGI	CCWGG	6 cut(s)	223, 689, 1654, 1821, 1906, 1933
PspN4I	GGNNCC	7 cut(s)	295, 596, 688, 779, 1007, 1660, 1932
PspPI	GGNCC	9 cut(s)	221, 227, 595, 778, 1103, 1535, 1658, 1825, 1931
PspPPI	RGGWCCY	2 cut(s)	778, 1658
PsrI	GAACNNNNNNTAC	2 cut(s)	1162, 1194
PsuI	RGATCY	6 cut(s)	674, 1023, 1192, 1269, 1409, 1952
PsyI	GACNNNGTC	1 cut(s)	652
RsaI	GTAC	4 cut(s)	361, 367, 1086, 1180
RsaNI	GTAC	4 cut(s)	360, 366, 1085, 1179
RseI	CAYNNNNRTG	4 cut(s)	203, 560, 649, 957
SaqAI	TTAA	7 cut(s)	69, 489, 1044, 1326, 1422, 1620, 1919
SatI	GCNGC	3 cut(s)	24, 142, 1378
Sau96I	GGNCC	9 cut(s)	221, 227, 595, 778, 1103, 1535, 1658, 1825, 1931
SchI	GAGTC	4 cut(s)	799, 1586, 1650, 1823
ScrFI	CCNGG	7 cut(s)	225, 684, 691, 1656, 1823, 1908, 1935
SduI	GDGCHC	2 cut(s)	93, 264
SfaNI	GCATC	1 cut(s)	946
SfcI	CTRYAG	1 cut(s)	1881
Sfr274I	CTCGAG	1 cut(s)	540
SinI	GGWCC	6 cut(s)	227, 595, 778, 1103, 1658, 1931
SlaI	CTCGAG	1 cut(s)	540
SmiMI	CAYNNNNRTG	4 cut(s)	203, 560, 649, 957
SmlI	CTYRAG	4 cut(s)	179, 540, 1308, 1672
SmoI	CTYRAG	4 cut(s)	179, 540, 1308, 1672
Sse9I	AATT	8 cut(s)	419, 457, 558, 987, 1396, 1499, 1719, 1801
SsiI	CCGC	3 cut(s)	141, 144, 1611
SspI	AATATT	1 cut(s)	1285
SspMI	CTAG	4 cut(s)	231, 257, 482, 782
StyD4I	CCNGG	7 cut(s)	223, 682, 689, 1654, 1821, 1906, 1933
StyI	CCWWGG	1 cut(s)	1300
TaaI	ACNGT	5 cut(s)	548, 599, 709, 1125, 1464
TaqI	TCGA	7 cut(s)	541, 656, 1197, 1590, 1754, 1849, 1867
TaqII	GACCGA	1 cut(s)	1849
TasI	AATT	8 cut(s)	419, 457, 558, 987, 1396, 1499, 1719, 1801
TatI	WGTACW	1 cut(s)	1178
TauI	GCSGC	1 cut(s)	144
TfiI	GAWTC	8 cut(s)	325, 843, 1072, 1109, 1199, 1276, 1292, 1756
Tru1I	TTAA	7 cut(s)	69, 489, 1044, 1326, 1422, 1620, 1919
Tru9I	TTAA	7 cut(s)	69, 489, 1044, 1326, 1422, 1620, 1919
TscAI	CASTG	2 cut(s)	651, 1007
TseFI	GTSAC	4 cut(s)	331, 517, 1369, 1439
TseI	GCWGC	2 cut(s)	23, 1377
Tsp45I	GTSAC	4 cut(s)	331, 517, 1369, 1439
TspDTI	ATGAA	6 cut(s)	429, 464, 653, 789, 1230, 1307
TspGWI	ACGGA	2 cut(s)	903, 1116
TspRI	CASTG	2 cut(s)	651, 1007
Tth111I	GACNNNGTC	1 cut(s)	652
Van91I	CCANNNNNTGG	3 cut(s)	342, 501, 1949
VpaK11BI	GGWCC	6 cut(s)	227, 595, 778, 1103, 1658, 1931
XapI	RAATTY	4 cut(s)	987, 1396, 1719, 1801
XbaI	TCTAGA	1 cut(s)	481
XhoI	CTCGAG	1 cut(s)	540
XmiI	GTMKAC	2 cut(s)	885, 1486
XspI	CTAG	4 cut(s)	231, 257, 482, 782
Zsp2I	ATGCAT	1 cut(s)	153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.