FvH4_6g10300

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013031
Physical Location & Seq
Forward (+)
6136097 .. 6137911
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10300.t1

Sequence Viewer

Length: 918 bp
ATGCCTGCAGTTATGCCTGAAAAACCCCTTCACGGCATTCATGGATTGGGAAGGGACAATACTACTACTCCTCCACATCCTAGAAGGTTGGAGGGGAAAATTGCTATAGTAACAGGTGCTGCACGAGGCATAGGAGAAGCAACAGTGAAGCTTTTCGCAAAGCACGGCGCCAAAGTTGTCATTGCCGATGTGGAAGACTCCACCGGCACAGCCTTGGCCAATTCCTTAGGCCCTTCAGTCACCTTCGTTCACTGCGACGTCAGCCTAGAAGAAGACATCGAAAACCTAATCCAGTCCACTGTTTCCCGCTACGGCCAGCTCGACATTATGTTCAATAACGCCGGAATCCTTGGAAACCAATCCAAACACAAGAGCATCGTCAACTTCGACGTCGACGAGTTCGACCGCGTGATGCGCGTGAACGTGAGAGGCACTGCATTGGGGATCAAGCATGCGGCGCGCGTGATGATCCCCGGGGGAGGAGGCTGCATAATCTCCACTGCCAGCGTCGCGGGAGTCGCCGGCGGGCTAGGGCCGCATGCCTACACTGCTTCCAAGCATGCCATTGTTGGCTTGACGAAGAACGCTGCGTGTGAACTAGGCCGGTATGGGATCAGAGTGAACTGTGTTTCTCCTTTCGGGGTTGCCACGCCGATGCTAGTGAATGCGTGGAGGATGAGTGACGAGGACGATGAGGAAGAAGAGTGTATGGACTTTGGGATGCCTTGTGAGCAAGAGGTGGAGAAGATGGAAGAGTTTGTGAGAGGGCTTGCGAATTTGAAAGGTCCGACTTTGAGGACTAAGGACATAGCCGAGGCAGCTCTATATCTTGCTAGTGATGAGTCCAAGTATGTAAGTGGTCATAATCTGGTTGTGGATGGTGGAATTACTACCTCAAGAAATTGTGTTGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

306

Amino Acids

32.37

Weight (kDa)

5.7

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 33 - 224 1.4e-51 short chain dehydrogenase
KR PF08659 36 - 189 1.2e-10 KR domain
adh_short_C2 PF13561 39 - 297 1.7e-59 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 261, 393
AccB1I GGYRCC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 393
AccII CGCG 5 cut(s) 408, 417, 460, 462, 512
AciI CCGC 6 cut(s) 307, 406, 455, 512, 525, 536
AclWI GGATC 3 cut(s) 452, 463, 620
AcoI YGGCCR 2 cut(s) 216, 313
AcsI RAATTY 1 cut(s) 775
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 3 cut(s) 168, 258, 390
AfiI CCNNNNNNNGG 2 cut(s) 32, 479
AgsI TTSAA 2 cut(s) 334, 781
AluBI AGCT 3 cut(s) 151, 319, 821
AluI AGCT 3 cut(s) 151, 319, 821
AlwI GGATC 3 cut(s) 452, 463, 620
AlwNI CAGNNNCTG 1 cut(s) 119
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 5 cut(s) 216, 229, 313, 533, 601
ApeKI GCWGC 4 cut(s) 119, 486, 587, 818
ApoI RAATTY 1 cut(s) 775
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 4 cut(s) 170, 417, 460, 462
AspS9I GGNCC 3 cut(s) 230, 533, 785
AsuC2I CCSGG 2 cut(s) 474, 475
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 785
AxyI CCTNAGG 1 cut(s) 226
BalI TGGCCA 1 cut(s) 218
BanI GGYRCC 1 cut(s) 167
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 201, 279
BbvI GCAGC 4 cut(s) 106, 473, 574, 830
BccI CCATC 2 cut(s) 742, 872
BceAI ACGGC 3 cut(s) 49, 181, 328
BcnI CCSGG 2 cut(s) 474, 475
BfaI CTAG 6 cut(s) 81, 266, 530, 599, 659, 834
BfmI CTRYAG 2 cut(s) 6, 105
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 6 cut(s) 120, 456, 487, 536, 588, 819
BlsI GCNGC 6 cut(s) 121, 457, 488, 537, 589, 820
Bme1390I CCNGG 2 cut(s) 474, 475
Bme18I GGWCC 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 3 cut(s) 230, 533, 785
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 2 cut(s) 474, 475
BmsI GCATC 4 cut(s) 384, 402, 645, 711
BpiI GAAGAC 2 cut(s) 201, 279
BpuEI CTTGAG 1 cut(s) 880
BpuMI CCSGG 2 cut(s) 474, 475
BsaHI GRCGYC 3 cut(s) 168, 258, 390
BsaJI CCNNGG 6 cut(s) 213, 349, 472, 473, 474, 813
BsaXI ACNNNNNCTCC 6 cut(s) 52, 55, 82, 85, 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 479
Bse118I RCCGGY 3 cut(s) 203, 521, 603
Bse1I ACTGG 1 cut(s) 292
Bse21I CCTNAGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 180
BseDI CCNNGG 6 cut(s) 213, 349, 472, 473, 474, 813
BseGI GGATG 4 cut(s) 76, 681, 726, 883
BseLI CCNNNNNNNGG 2 cut(s) 32, 479
BseMI GCAATG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 292
BsePI GCGCGC 1 cut(s) 458
BseRI GAGGAG 2 cut(s) 60, 495
BseXI GCAGC 4 cut(s) 106, 473, 574, 830
BsgI GTGCAG 1 cut(s) 105
Bsh1236I CGCG 5 cut(s) 408, 417, 460, 462, 512
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 5 cut(s) 218, 231, 315, 535, 603
BshNI GGYRCC 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 5 cut(s) 204, 342, 474, 522, 604
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 32, 479
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 2 cut(s) 36, 670
BsnI GGCC 5 cut(s) 218, 231, 315, 535, 603
BsoBI CYCGRG 1 cut(s) 473
Bsp143I GATC 3 cut(s) 444, 468, 612
BspACI CCGC 6 cut(s) 307, 406, 455, 512, 525, 536
BspANI GGCC 5 cut(s) 218, 231, 315, 535, 603
BspFNI CGCG 5 cut(s) 408, 417, 460, 462, 512
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 3 cut(s) 452, 463, 620
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 180
BsrFI RCCGGY 3 cut(s) 203, 521, 603
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 3 cut(s) 203, 521, 603
BssECI CCNNGG 6 cut(s) 213, 349, 472, 473, 474, 813
BssHII GCGCGC 1 cut(s) 458
BssMI GATC 3 cut(s) 444, 468, 612
BssNI GRCGYC 3 cut(s) 168, 258, 390
BssSI CACGAG 1 cut(s) 123
BssT1I CCWWGG 2 cut(s) 213, 349
Bst2BI CACGAG 1 cut(s) 123
Bst4CI ACNGT 3 cut(s) 145, 301, 626
Bst6I CTCTTC 2 cut(s) 696, 747
BstACI GRCGYC 3 cut(s) 168, 258, 390
BstDEI CTNAG 2 cut(s) 226, 801
BstF5I GGATG 4 cut(s) 76, 681, 726, 883
BstFNI CGCG 5 cut(s) 408, 417, 460, 462, 512
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 4 cut(s) 170, 417, 460, 462
BstKTI GATC 3 cut(s) 447, 471, 615
BstMBI GATC 3 cut(s) 444, 468, 612
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 9 cut(s) 261, 414, 457, 509, 518, 535, 548, 730, 818
BstNSI RCATGY 3 cut(s) 455, 542, 563
BstSCI CCNGG 2 cut(s) 472, 473
BstSFI CTRYAG 2 cut(s) 6, 105
BstUI CGCG 5 cut(s) 408, 417, 460, 462, 512
BstV1I GCAGC 4 cut(s) 106, 473, 574, 830
BstV2I GAAGAC 2 cut(s) 201, 279
Bsu36I CCTNAGG 1 cut(s) 226
BsuRI GGCC 5 cut(s) 218, 231, 315, 535, 603
BtsCI GGATG 4 cut(s) 76, 681, 726, 883
BtsI GCAGTG 4 cut(s) 250, 432, 498, 546
BtsIMutI CAGTG 6 cut(s) 150, 250, 297, 432, 498, 546
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 4 cut(s) 170, 417, 460, 462
Cfr10I RCCGGY 3 cut(s) 203, 521, 603
Cfr13I GGNCC 3 cut(s) 230, 533, 785
Cfr9I CCCGGG 1 cut(s) 473
CseI GACGC 1 cut(s) 496
CviAII CATG 4 cut(s) 41, 452, 539, 560
DdeI CTNAG 2 cut(s) 226, 801
DinI GGCGCC 1 cut(s) 169
DpnI GATC 3 cut(s) 446, 470, 614
DpnII GATC 3 cut(s) 444, 468, 612
EaeI YGGCCR 2 cut(s) 216, 313
Eam1104I CTCTTC 2 cut(s) 696, 747
EarI CTCTTC 2 cut(s) 696, 747
Eco130I CCWWGG 2 cut(s) 213, 349
Eco47I GGWCC 1 cut(s) 785
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 213, 349
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 2 cut(s) 213, 349
FaeI CATG 4 cut(s) 44, 455, 542, 563
FaqI GGGAC 1 cut(s) 68
FatI CATG 4 cut(s) 40, 451, 538, 559
FauI CCCGC 3 cut(s) 314, 505, 518
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 6 cut(s) 120, 456, 487, 536, 588, 819
FokI GGATG 4 cut(s) 63, 688, 733, 890
Fsp4HI GCNGC 6 cut(s) 120, 456, 487, 536, 588, 819
FspBI CTAG 6 cut(s) 81, 266, 530, 599, 659, 834
GlaI GCGC 4 cut(s) 169, 416, 459, 461
GluI GCNGC 6 cut(s) 120, 456, 487, 536, 588, 819
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 5 cut(s) 218, 231, 315, 535, 603
HapII CCGG 5 cut(s) 204, 342, 474, 522, 604
HgaI GACGC 1 cut(s) 496
HhaI GCGC 4 cut(s) 170, 417, 460, 462
Hin1I GRCGYC 3 cut(s) 168, 258, 390
Hin1II CATG 4 cut(s) 44, 455, 542, 563
Hin6I GCGC 4 cut(s) 168, 415, 458, 460
HinP1I GCGC 4 cut(s) 168, 415, 458, 460
HincII GTYRAC 2 cut(s) 382, 394
HindII GTYRAC 2 cut(s) 382, 394
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 197, 345, 516, 842
HpaII CCGG 5 cut(s) 204, 342, 474, 522, 604
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 7 cut(s) 250, 297, 382, 394, 421, 596, 622
Hpy188I TCNGA 2 cut(s) 617, 789
Hpy188III TCNNGA 1 cut(s) 897
Hpy8I GTNNAC 7 cut(s) 250, 297, 382, 394, 421, 596, 622
Hpy99I CGWCG 5 cut(s) 260, 392, 395, 398, 512
HpyAV CCTTC 5 cut(s) 38, 45, 78, 243, 253
HpyCH4III ACNGT 3 cut(s) 145, 301, 626
HpyCH4IV ACGT 3 cut(s) 258, 390, 423
HpyCH4V TGCA 4 cut(s) 8, 122, 437, 489
HpyF10VI GCNNNNNNNGC 9 cut(s) 261, 414, 457, 509, 518, 535, 548, 730, 818
HpyF3I CTNAG 2 cut(s) 226, 801
HpySE526I ACGT 3 cut(s) 258, 390, 423
Hsp92I GRCGYC 3 cut(s) 168, 258, 390
Hsp92II CATG 4 cut(s) 44, 455, 542, 563
HspAI GCGC 4 cut(s) 168, 415, 458, 460
KasI GGCGCC 1 cut(s) 167
KroI GCCGGC 1 cut(s) 521
KroNI GCCGGC 1 cut(s) 523
Kzo9I GATC 3 cut(s) 444, 468, 612
Lsp1109I GCAGC 4 cut(s) 106, 473, 574, 830
LweI GCATC 4 cut(s) 384, 402, 645, 711
MaeI CTAG 6 cut(s) 81, 266, 530, 599, 659, 834
MaeII ACGT 3 cut(s) 258, 390, 423
MaeIII GTNAC 3 cut(s) 109, 238, 680
MalI GATC 3 cut(s) 446, 470, 614
MboI GATC 3 cut(s) 444, 468, 612
MboII GAAGA 8 cut(s) 206, 281, 284, 592, 710, 713, 757, 764
MlsI TGGCCA 1 cut(s) 218
MluCI AATT 5 cut(s) 99, 220, 775, 885, 901
MluNI TGGCCA 1 cut(s) 218
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 3 cut(s) 191, 525, 851
MmeI TCCRAC 2 cut(s) 69, 812
Mox20I TGGCCA 1 cut(s) 218
MreI CGCCGGCG 1 cut(s) 521
MroNI GCCGGC 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 218
MslI CAYNNNNRTG 1 cut(s) 653
Msp20I TGGCCA 1 cut(s) 218
MspI CCGG 5 cut(s) 204, 342, 474, 522, 604
MspR9I CCNGG 2 cut(s) 474, 475
Mva1269I GAATGC 2 cut(s) 36, 670
MvnI CGCG 5 cut(s) 408, 417, 460, 462, 512
MwoI GCNNNNNNNGC 9 cut(s) 261, 414, 457, 509, 518, 535, 548, 730, 818
NaeI GCCGGC 1 cut(s) 523
NarI GGCGCC 1 cut(s) 168
NciI CCSGG 2 cut(s) 474, 475
NdeII GATC 3 cut(s) 444, 468, 612
NgoMIV GCCGGC 1 cut(s) 521
NlaIII CATG 4 cut(s) 44, 455, 542, 563
NlaIV GGNNCC 1 cut(s) 169
NmeAIII GCCGAG 1 cut(s) 838
NmuCI GTSAC 2 cut(s) 238, 680
NspI RCATGY 3 cut(s) 455, 542, 563
PaeI GCATGC 3 cut(s) 455, 542, 563
PauI GCGCGC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 5 cut(s) 252, 318, 384, 393, 399
PctI GAATGC 2 cut(s) 36, 670
PdiI GCCGGC 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 345
PkrI GCNGC 6 cut(s) 121, 457, 488, 537, 589, 820
PleI GAGTC 3 cut(s) 191, 524, 850
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 3 cut(s) 191, 524, 850
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 3 cut(s) 230, 533, 785
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 119
PteI GCGCGC 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 653
SalI GTCGAC 1 cut(s) 392
SatI GCNGC 6 cut(s) 120, 456, 487, 536, 588, 819
Sau3AI GATC 3 cut(s) 444, 468, 612
Sau96I GGNCC 3 cut(s) 230, 533, 785
SchI GAGTC 3 cut(s) 191, 525, 851
ScrFI CCNGG 2 cut(s) 474, 475
SfaNI GCATC 4 cut(s) 384, 402, 645, 711
SfcI CTRYAG 2 cut(s) 6, 105
SfoI GGCGCC 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 521
SgrDI CGTCGACG 1 cut(s) 392
SinI GGWCC 1 cut(s) 785
SmaI CCCGGG 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 895
SmoI CTYRAG 1 cut(s) 895
SphI GCATGC 3 cut(s) 455, 542, 563
Sse9I AATT 5 cut(s) 99, 220, 775, 885, 901
SsiI CCGC 6 cut(s) 307, 406, 455, 512, 525, 536
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 6 cut(s) 81, 266, 530, 599, 659, 834
StyD4I CCNGG 2 cut(s) 472, 473
StyI CCWWGG 2 cut(s) 213, 349
TaaI ACNGT 3 cut(s) 145, 301, 626
TaiI ACGT 3 cut(s) 261, 393, 426
TaqI TCGA 5 cut(s) 279, 321, 387, 393, 402
TasI AATT 5 cut(s) 99, 220, 775, 885, 901
TauI GCSGC 2 cut(s) 458, 538
TfiI GAWTC 1 cut(s) 345
TscAI CASTG 6 cut(s) 150, 257, 304, 439, 505, 553
TseFI GTSAC 2 cut(s) 238, 680
TseI GCWGC 4 cut(s) 119, 486, 587, 818
Tsp45I GTSAC 2 cut(s) 238, 680
TspDTI ATGAA 1 cut(s) 29
TspMI CCCGGG 1 cut(s) 473
TspRI CASTG 6 cut(s) 150, 257, 304, 439, 505, 553
VpaK11BI GGWCC 1 cut(s) 785
XapI RAATTY 1 cut(s) 775
XceI RCATGY 3 cut(s) 455, 542, 563
XmaI CCCGGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 6 cut(s) 81, 266, 530, 599, 659, 834
ZraI GACGTC 2 cut(s) 259, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.