MD12G1181900.v1.1

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013031
Physical Location & Seq
Forward (+)
26162654 .. 26163953
1300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1181900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGCGTGCCCAAGTTATGCCAGAAAAAGCCCTTCATGGAATCCATGGATTGGGAAGGGACAACATTACTACTACTCCTCCCCCCAAAAGGTTGGATGGAAAAGTTGCTATAGTGACAGGTGGTGCTAGAGGCATTGGAGAAGCAACAGTGAGGCTTTTTGCAAGGCATGGCGCCAAAGCAGTCATTGCTGACGTGGAGGATGCAGTTGGCACAGCCCTAGCAAATTCCCTGGGCCCTTCAGTTACCTTTGTTCACTGTGATGTTAGCTTGGAAGAAGACATGGAAAACCTAATTGAATCAACAATTTCCCGTTACGGGCAACTGGACATTATGTTCAACAATGCCGGGGTCCTCGGAAACCAGTCGAAACACAAGAGCATCATGAACTTTGATGTCGATGAATTTGACCGCGTAATGCGGGTGAATGTTAGAGGAGCTGCATTGGGGATGAAGCACGCTGCAAGAGTGATGGTTGCTAGAGGAATCGGAGGGTGCATAATTTCAACTGCAAGTGTAGCCGGAGTAGCAGGCGGCCTGGGGCCACATGCTTACACAGCTTCAAAACATGCCATTGTAGGGCTCACAAAGAACACAGCTTGTGAGCTGGGAAGGTACGGAATTAGGGTAAACTGCATTTCTCCGTTTGGGGTTGCCACGTCAATGCTGGTCAACGCTTGGAGGATGAGTAGCGATGGTGATGATGAAGATGAAGAGGAATGTACGGATTTGGGGATGCCTTGTGAGCAAGAAGTGGAGAAAATGGAAGAGTTTGTGAGAGGGCTTGCCAATTTAAAAGGTCAAACTTTGAGGACAAAAGACATAGCTGAGGCTGCTCTTTATCTTGCTAGTGATGAATCCAAGTACGTTAGTGGCCATAACTTGGTTGTGGATGGTGGAATTACCACTTCAAGAAATTGTGTTGGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

310

Amino Acids

32.79

Weight (kDa)

5.74

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 34 - 225 1.7e-48 short chain dehydrogenase
KR PF08659 37 - 192 1.2e-11 KR domain
adh_short_C2 PF13561 41 - 225 8.6e-47 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 251 - 301 1.9e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccB7I CCANNNNNTGG 2 cut(s) 49, 880
AccII CGCG 1 cut(s) 411
AciI CCGC 3 cut(s) 409, 418, 531
AcoI YGGCCR 1 cut(s) 871
AcsI RAATTY 2 cut(s) 223, 401
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 3 cut(s) 614, 721, 863
AfiI CCNNNNNNNGG 5 cut(s) 49, 87, 315, 576, 880
AgsI TTSAA 5 cut(s) 296, 337, 504, 561, 909
AjiI CACGTC 2 cut(s) 193, 657
AjnI CCWGG 2 cut(s) 228, 534
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
AluBI AGCT 6 cut(s) 267, 437, 557, 596, 604, 824
AluI AGCT 6 cut(s) 267, 437, 557, 596, 604, 824
AoxI GGCC 4 cut(s) 232, 532, 539, 871
ApaI GGGCCC 1 cut(s) 236
ApeKI GCWGC 3 cut(s) 437, 458, 830
ApoI RAATTY 2 cut(s) 223, 401
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 4 cut(s) 232, 233, 349, 539
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 433, 707
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 2 cut(s) 10, 236
BalI TGGCCA 1 cut(s) 873
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 2 cut(s) 236, 582
BbsI GAAGAC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 825
BbvI GCAGC 3 cut(s) 424, 445, 817
BccI CCATC 4 cut(s) 89, 463, 686, 884
BciT130I CCWGG 2 cut(s) 230, 536
BcnI CCSGG 1 cut(s) 346
BfaI CTAG 4 cut(s) 126, 218, 477, 846
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 4 cut(s) 438, 459, 532, 831
BlsI GCNGC 4 cut(s) 439, 460, 533, 832
Bme1390I CCNGG 3 cut(s) 230, 346, 536
Bme18I GGWCC 1 cut(s) 349
BmgBI CACGTC 2 cut(s) 193, 657
BmgT120I GGNCC 4 cut(s) 232, 233, 349, 539
BmiI GGNNCC 4 cut(s) 172, 234, 350, 540
BmrFI CCNGG 3 cut(s) 230, 346, 536
BmsI GCATC 3 cut(s) 190, 387, 723
BpiI GAAGAC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 825
BpuMI CCSGG 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 6 cut(s) 43, 228, 229, 345, 352, 535
BsaXI ACNNNNNCTCC 6 cut(s) 58, 61, 88, 91, 670, 700
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 87, 315, 576, 880
Bse1I ACTGG 2 cut(s) 327, 361
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 2 cut(s) 230, 536
BseDI CCNNGG 6 cut(s) 43, 228, 229, 345, 352, 535
BseGI GGATG 6 cut(s) 100, 205, 453, 687, 738, 895
BseLI CCNNNNNNNGG 5 cut(s) 49, 87, 315, 576, 880
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 327, 361
BseRI GAGGAG 2 cut(s) 66, 447
BseSI GKGCMC 2 cut(s) 10, 236
BseXI GCAGC 3 cut(s) 424, 445, 817
BseYI CCCAGC 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 411
BshFI GGCC 4 cut(s) 234, 534, 541, 873
BshNI GGYRCC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 345, 519
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 5 cut(s) 49, 87, 315, 576, 880
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 4 cut(s) 234, 534, 541, 873
Bsp120I GGGCCC 1 cut(s) 232
Bsp1286I GDGCHC 3 cut(s) 10, 236, 582
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 3 cut(s) 409, 418, 531
BspANI GGCC 4 cut(s) 234, 534, 541, 873
BspCNI CTCAG 1 cut(s) 817
BspFNI CGCG 1 cut(s) 411
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 4 cut(s) 172, 234, 350, 540
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 2 cut(s) 327, 361
BssECI CCNNGG 6 cut(s) 43, 228, 229, 345, 352, 535
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 43
Bst2UI CCWGG 2 cut(s) 230, 536
Bst4CI ACNGT 2 cut(s) 148, 257
Bst6I CTCTTC 2 cut(s) 705, 759
BstACI GRCGYC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 4 cut(s) 6, 456, 529, 783
BstDEI CTNAG 1 cut(s) 825
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 6 cut(s) 100, 205, 453, 687, 738, 895
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 1 cut(s) 173
BstMWI GCNNNNNNNGC 6 cut(s) 185, 515, 524, 554, 742, 830
BstNI CCWGG 2 cut(s) 230, 536
BstNSI RCATGY 2 cut(s) 548, 569
BstSCI CCNGG 3 cut(s) 228, 344, 534
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 2 cut(s) 10, 236
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 424, 445, 817
BstV2I GAAGAC 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 91
BsuRI GGCC 4 cut(s) 234, 534, 541, 873
BtgI CCRYGG 1 cut(s) 43
BtgZI GCGATG 1 cut(s) 705
BtrI CACGTC 2 cut(s) 193, 657
BtsCI GGATG 6 cut(s) 100, 205, 453, 687, 738, 895
BtsIMutI CAGTG 2 cut(s) 153, 253
Cac8I GCNNGC 4 cut(s) 6, 456, 529, 783
CciI TCATGA 1 cut(s) 381
CfoI GCGC 1 cut(s) 173
Cfr13I GGNCC 4 cut(s) 232, 233, 349, 539
Csp6I GTAC 3 cut(s) 613, 720, 862
CviAII CATG 7 cut(s) 35, 44, 167, 280, 382, 545, 566
CviQI GTAC 3 cut(s) 613, 720, 862
DdeI CTNAG 1 cut(s) 825
DinI GGCGCC 1 cut(s) 172
DraI TTTAAA 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 871
Eam1104I CTCTTC 2 cut(s) 705, 759
EarI CTCTTC 2 cut(s) 705, 759
Eco130I CCWWGG 1 cut(s) 43
Eco24I GRGCYC 2 cut(s) 236, 582
Eco47I GGWCC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 222
EcoO109I RGGNCCY 2 cut(s) 233, 349
EcoRII CCWGG 2 cut(s) 228, 534
EcoT14I CCWWGG 1 cut(s) 43
EcoT38I GRGCYC 2 cut(s) 236, 582
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 7 cut(s) 38, 47, 170, 283, 385, 548, 569
FaqI GGGAC 1 cut(s) 71
FatI CATG 7 cut(s) 34, 43, 166, 279, 381, 544, 565
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 438, 459, 532, 831
FokI GGATG 6 cut(s) 107, 212, 460, 694, 745, 902
FriOI GRGCYC 2 cut(s) 236, 582
Fsp4HI GCNGC 4 cut(s) 438, 459, 532, 831
FspBI CTAG 4 cut(s) 126, 218, 477, 846
GlaI GCGC 1 cut(s) 172
GluI GCNGC 4 cut(s) 438, 459, 532, 831
GsaI CCCAGC 1 cut(s) 608
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 4 cut(s) 234, 534, 541, 873
HapII CCGG 2 cut(s) 345, 519
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 7 cut(s) 38, 47, 170, 283, 385, 548, 569
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HinfI GANTC 4 cut(s) 39, 296, 483, 854
HpaII CCGG 2 cut(s) 345, 519
HphI GGTGA 2 cut(s) 433, 707
Hpy166II GTNNAC 3 cut(s) 253, 628, 670
Hpy188I TCNGA 2 cut(s) 356, 488
Hpy188III TCNNGA 2 cut(s) 382, 909
Hpy8I GTNNAC 3 cut(s) 253, 628, 670
HpyAV CCTTC 4 cut(s) 41, 48, 246, 603
HpyCH4III ACNGT 2 cut(s) 148, 257
HpyCH4IV ACGT 3 cut(s) 192, 656, 864
HpyCH4V TGCA 7 cut(s) 161, 203, 440, 461, 495, 509, 633
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 515, 524, 554, 742, 830
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 3 cut(s) 192, 656, 864
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 7 cut(s) 38, 47, 170, 283, 385, 548, 569
HspAI GCGC 1 cut(s) 171
KasI GGCGCC 1 cut(s) 170
LmnI GCTCC 1 cut(s) 434
Lsp1109I GCAGC 3 cut(s) 424, 445, 817
LweI GCATC 3 cut(s) 190, 387, 723
MaeI CTAG 4 cut(s) 126, 218, 477, 846
MaeII ACGT 3 cut(s) 192, 656, 864
MaeIII GTNAC 3 cut(s) 112, 241, 311
MboII GAAGA 5 cut(s) 284, 287, 716, 722, 776
MhlI GDGCHC 3 cut(s) 10, 236, 582
MlsI TGGCCA 1 cut(s) 873
MluCI AATT 9 cut(s) 223, 291, 303, 401, 498, 618, 787, 897, 913
MluNI TGGCCA 1 cut(s) 873
Mly113I GGCGCC 1 cut(s) 171
MmeI TCCRAC 1 cut(s) 72
Mox20I TGGCCA 1 cut(s) 873
MscI TGGCCA 1 cut(s) 873
MseI TTAA 1 cut(s) 791
MslI CAYNNNNRTG 2 cut(s) 258, 659
Msp20I TGGCCA 1 cut(s) 873
MspI CCGG 2 cut(s) 345, 519
MspR9I CCNGG 3 cut(s) 230, 346, 536
MvaI CCWGG 2 cut(s) 230, 536
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 6 cut(s) 185, 515, 524, 554, 742, 830
NarI GGCGCC 1 cut(s) 171
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 43
NlaIII CATG 7 cut(s) 38, 47, 170, 283, 385, 548, 569
NlaIV GGNNCC 4 cut(s) 172, 234, 350, 540
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 548, 569
PagI TCATGA 1 cut(s) 381
PasI CCCWGGG 1 cut(s) 229
PfeI GAWTC 4 cut(s) 39, 296, 483, 854
PflMI CCANNNNNTGG 2 cut(s) 49, 880
PkrI GCNGC 4 cut(s) 439, 460, 533, 832
PluTI GGCGCC 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 228, 534
PspFI CCCAGC 1 cut(s) 604
PspGI CCWGG 2 cut(s) 228, 534
PspN4I GGNNCC 4 cut(s) 172, 234, 350, 540
PspOMI GGGCCC 1 cut(s) 232
PspPI GGNCC 4 cut(s) 232, 233, 349, 539
PspPPI RGGWCCY 1 cut(s) 349
RsaI GTAC 3 cut(s) 614, 721, 863
RsaNI GTAC 3 cut(s) 613, 720, 862
RseI CAYNNNNRTG 2 cut(s) 258, 659
SaqAI TTAA 1 cut(s) 791
SatI GCNGC 4 cut(s) 438, 459, 532, 831
Sau96I GGNCC 4 cut(s) 232, 233, 349, 539
ScrFI CCNGG 3 cut(s) 230, 346, 536
SduI GDGCHC 3 cut(s) 10, 236, 582
SfaNI GCATC 3 cut(s) 190, 387, 723
SfcI CTRYAG 1 cut(s) 108
SfoI GGCGCC 1 cut(s) 172
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 2 cut(s) 258, 659
Sse9I AATT 9 cut(s) 223, 291, 303, 401, 498, 618, 787, 897, 913
SsiI CCGC 3 cut(s) 409, 418, 531
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 4 cut(s) 126, 218, 477, 846
StyD4I CCNGG 3 cut(s) 228, 344, 534
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 2 cut(s) 148, 257
TaiI ACGT 3 cut(s) 195, 659, 867
TaqI TCGA 2 cut(s) 365, 396
TasI AATT 9 cut(s) 223, 291, 303, 401, 498, 618, 787, 897, 913
TauI GCSGC 1 cut(s) 534
TfiI GAWTC 4 cut(s) 39, 296, 483, 854
Tru1I TTAA 1 cut(s) 791
Tru9I TTAA 1 cut(s) 791
TscAI CASTG 2 cut(s) 153, 260
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 437, 458, 830
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 7 cut(s) 23, 398, 414, 464, 717, 723, 867
TspGWI ACGGA 3 cut(s) 630, 630, 737
TspRI CASTG 2 cut(s) 153, 260
Van91I CCANNNNNTGG 2 cut(s) 49, 880
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 2 cut(s) 223, 401
XceI RCATGY 2 cut(s) 548, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XspI CTAG 4 cut(s) 126, 218, 477, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.