RLG00000024955

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013031
Physical Location & Seq
Reverse (-)
40752971 .. 40754169
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024955

Sequence Viewer

Length: 921 bp
ATGCCTGCCGTAATGCCTGAAACACCCCTTCATGGAATTCATGGATTGGGAAGGGACAACAGTACTACTACTCCTGCACATCCTAGAAGGTTGGAGGGCAAAGTTGCTATAGTAACGGGCGGTGCACGAGGCATAGGAGAAGCAACAGCGAGGCTTTTCACAAGGCACGGCGCCAAAGTAGTCATTGCCGATGTGGAGGACTCTATCGGCACAGCCCTGGCCAATTCCTTAGGACCATCAGTCACCTTCGTTCACTGCGACGTCAGCCTCGAAGAAGACATCGAAAACCTAATCGAGTCCACCGTTTCCCGCTACGGGCAGCTCGACATTATGTTCAACAACGCCGGAATCCTTGGCAACCAATCCAAACACAAGAGCATCGTCAACTTTAATGTCGACGAGTTCGACCGCGTGATGCGCGTGAACGTGAGAGGCACTGCCTTGGGGATCAAGCATGCGGCGCGTGTGATGATCCCCAGGGGTGGAGGCTGCATTATTTCAACCGCGAGCGTCGCCGGAGTAGCTGGTGGGTTAGGGCCGCATGCCTACACAGCTTCCAAGCATGCCATTGTTGGGCTGACGAAGAACGCCGCCTGTGAACTAGGCCGGTATGGGATCAGAGTGAACTGTATTTCGCCTTTTGGAGTTGCCACGCCGATGCTGGTGAATGCGTGGAGGATGAGTAACGATGACGAAGAGGAAGAAAAGTGTATGGATTTTGGGATGCCTTGTGAGCAAGAGGTGGAGAAGATGGAAGATTTCGTGAGAGGGCTTGCGAATTTGAAAGGCCCGATTTTGAGGGCTAAGGACATAGCCGAGGCTGCTCTATATCTTGCTAGTGATGAGTCCAAATATGTAAGTGGTCATAATCTGGTTGTGGATGGTGGAATTACTACCTCAAGAAATTGTGTTGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

307

Amino Acids

32.52

Weight (kDa)

6.07

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 34 - 225 9.4e-51 short chain dehydrogenase
KR PF08659 37 - 190 3e-11 KR domain
adh_short_C2 PF13561 41 - 226 7.7e-50 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 248 - 298 4.3e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccB1I GGYRCC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 396
AccII CGCG 4 cut(s) 411, 420, 463, 506
AciI CCGC 7 cut(s) 120, 310, 409, 458, 504, 539, 591
AclWI GGATC 3 cut(s) 455, 466, 623
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 2 cut(s) 36, 778
AcyI GRCGYC 2 cut(s) 171, 261
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 4 cut(s) 32, 315, 482, 573
AgsI TTSAA 3 cut(s) 337, 501, 784
AhdI GACNNNNNGTC 1 cut(s) 239
AjnI CCWGG 2 cut(s) 216, 476
AluBI AGCT 3 cut(s) 322, 524, 554
AluI AGCT 3 cut(s) 322, 524, 554
Alw21I GWGCWC 1 cut(s) 127
Alw44I GTGCAC 1 cut(s) 123
AlwI GGATC 3 cut(s) 455, 466, 623
AoxI GGCC 4 cut(s) 219, 536, 604, 787
ApaLI GTGCAC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 319, 489, 821
ApoI RAATTY 2 cut(s) 36, 778
AspLEI GCGC 3 cut(s) 173, 420, 463
AspS9I GGNCC 3 cut(s) 233, 536, 788
AsuHPI GGTGA 2 cut(s) 235, 676
AvaII GGWCC 1 cut(s) 233
AxyI CCTNAGG 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 221
BanI GGYRCC 1 cut(s) 170
BauI CACGAG 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 3 cut(s) 331, 476, 808
BccI CCATC 3 cut(s) 244, 745, 875
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 218, 478
BfaI CTAG 3 cut(s) 84, 602, 837
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 6 cut(s) 320, 459, 490, 539, 591, 822
BlsI GCNGC 6 cut(s) 321, 460, 491, 540, 592, 823
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 218, 478
Bme18I GGWCC 1 cut(s) 233
BmeRI GACNNNNNGTC 1 cut(s) 239
BmgT120I GGNCC 3 cut(s) 233, 536, 788
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 2 cut(s) 218, 478
BmsI GCATC 4 cut(s) 387, 405, 648, 714
BpiI GAAGAC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 804
BpuEI CTTGAG 1 cut(s) 883
BsaHI GRCGYC 2 cut(s) 171, 261
BsaJI CCNNGG 6 cut(s) 216, 352, 441, 476, 477, 816
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 667, 697
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 315, 482, 573
Bse118I RCCGGY 1 cut(s) 606
Bse21I CCTNAGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 2 cut(s) 218, 478
BseDI CCNNGG 6 cut(s) 216, 352, 441, 476, 477, 816
BseGI GGATG 4 cut(s) 79, 684, 729, 886
BseLI CCNNNNNNNGG 4 cut(s) 32, 315, 482, 573
BseMI GCAATG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 3 cut(s) 331, 476, 808
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 4 cut(s) 411, 420, 463, 506
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 4 cut(s) 221, 538, 606, 789
BshNI GGYRCC 1 cut(s) 170
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 3 cut(s) 345, 516, 607
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 4 cut(s) 32, 315, 482, 573
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 673
BsnI GGCC 4 cut(s) 221, 538, 606, 789
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 447, 471, 615
BspACI CCGC 7 cut(s) 120, 310, 409, 458, 504, 539, 591
BspANI GGCC 4 cut(s) 221, 538, 606, 789
BspFNI CGCG 4 cut(s) 411, 420, 463, 506
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 3 cut(s) 455, 466, 623
BspT107I GGYRCC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 6 cut(s) 216, 352, 441, 476, 477, 816
BssMI GATC 3 cut(s) 447, 471, 615
BssNI GRCGYC 2 cut(s) 171, 261
BssSI CACGAG 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 352, 441
Bst2BI CACGAG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 218, 478
Bst4CI ACNGT 3 cut(s) 62, 304, 629
Bst6I CTCTTC 1 cut(s) 690
BstACI GRCGYC 2 cut(s) 171, 261
BstC8I GCNNGC 6 cut(s) 6, 456, 508, 543, 564, 774
BstDEI CTNAG 2 cut(s) 229, 804
BstF5I GGATG 4 cut(s) 79, 684, 729, 886
BstFNI CGCG 4 cut(s) 411, 420, 463, 506
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 3 cut(s) 173, 420, 463
BstKTI GATC 3 cut(s) 450, 474, 618
BstMBI GATC 3 cut(s) 447, 471, 615
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 8 cut(s) 264, 417, 460, 512, 521, 551, 733, 821
BstNI CCWGG 2 cut(s) 218, 478
BstNSI RCATGY 3 cut(s) 458, 545, 566
BstSCI CCNGG 2 cut(s) 216, 476
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 127
BstUI CGCG 4 cut(s) 411, 420, 463, 506
BstV1I GCAGC 3 cut(s) 331, 476, 808
BstV2I GAAGAC 1 cut(s) 282
Bsu36I CCTNAGG 1 cut(s) 229
BsuRI GGCC 4 cut(s) 221, 538, 606, 789
BtsCI GGATG 4 cut(s) 79, 684, 729, 886
BtsI GCAGTG 2 cut(s) 253, 435
BtsIMutI CAGTG 2 cut(s) 253, 435
Cac8I GCNNGC 6 cut(s) 6, 456, 508, 543, 564, 774
CfoI GCGC 3 cut(s) 173, 420, 463
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 3 cut(s) 233, 536, 788
CseI GACGC 1 cut(s) 499
Csp6I GTAC 1 cut(s) 63
CviAII CATG 5 cut(s) 32, 41, 455, 542, 563
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 2 cut(s) 229, 804
DinI GGCGCC 1 cut(s) 172
DpnI GATC 3 cut(s) 449, 473, 617
DpnII GATC 3 cut(s) 447, 471, 615
DriI GACNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 690
Eam1105I GACNNNNNGTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 690
Eco130I CCWWGG 2 cut(s) 352, 441
Eco47I GGWCC 1 cut(s) 233
Eco81I CCTNAGG 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 2 cut(s) 216, 476
EcoT14I CCWWGG 2 cut(s) 352, 441
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 352, 441
FaeI CATG 5 cut(s) 35, 44, 458, 545, 566
FaqI GGGAC 1 cut(s) 68
FatI CATG 5 cut(s) 31, 40, 454, 541, 562
FauI CCCGC 1 cut(s) 317
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 6 cut(s) 320, 459, 490, 539, 591, 822
FokI GGATG 4 cut(s) 66, 691, 736, 893
Fsp4HI GCNGC 6 cut(s) 320, 459, 490, 539, 591, 822
FspBI CTAG 3 cut(s) 84, 602, 837
GlaI GCGC 3 cut(s) 172, 419, 462
GluI GCNGC 6 cut(s) 320, 459, 490, 539, 591, 822
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 4 cut(s) 221, 538, 606, 789
HapII CCGG 3 cut(s) 345, 516, 607
HgaI GACGC 1 cut(s) 499
HhaI GCGC 3 cut(s) 173, 420, 463
Hin1I GRCGYC 2 cut(s) 171, 261
Hin1II CATG 5 cut(s) 35, 44, 458, 545, 566
Hin6I GCGC 3 cut(s) 171, 418, 461
HinP1I GCGC 3 cut(s) 171, 418, 461
HincII GTYRAC 2 cut(s) 385, 397
HindII GTYRAC 2 cut(s) 385, 397
HinfI GANTC 4 cut(s) 200, 296, 348, 845
HpaII CCGG 3 cut(s) 345, 516, 607
HphI GGTGA 2 cut(s) 235, 676
Hpy166II GTNNAC 8 cut(s) 125, 253, 300, 385, 397, 424, 599, 625
Hpy188I TCNGA 1 cut(s) 620
Hpy188III TCNNGA 2 cut(s) 763, 900
Hpy8I GTNNAC 8 cut(s) 125, 253, 300, 385, 397, 424, 599, 625
Hpy99I CGWCG 3 cut(s) 263, 401, 515
HpyAV CCTTC 4 cut(s) 38, 45, 81, 256
HpyCH4III ACNGT 3 cut(s) 62, 304, 629
HpyCH4IV ACGT 2 cut(s) 261, 426
HpyCH4V TGCA 3 cut(s) 77, 125, 492
HpyF10VI GCNNNNNNNGC 8 cut(s) 264, 417, 460, 512, 521, 551, 733, 821
HpyF3I CTNAG 2 cut(s) 229, 804
HpySE526I ACGT 2 cut(s) 261, 426
Hsp92I GRCGYC 2 cut(s) 171, 261
Hsp92II CATG 5 cut(s) 35, 44, 458, 545, 566
HspAI GCGC 3 cut(s) 171, 418, 461
KasI GGCGCC 1 cut(s) 170
Kzo9I GATC 3 cut(s) 447, 471, 615
Lsp1109I GCAGC 3 cut(s) 331, 476, 808
LweI GCATC 4 cut(s) 387, 405, 648, 714
MaeI CTAG 3 cut(s) 84, 602, 837
MaeII ACGT 2 cut(s) 261, 426
MaeIII GTNAC 3 cut(s) 112, 241, 683
MalI GATC 3 cut(s) 449, 473, 617
MboI GATC 3 cut(s) 447, 471, 615
MboII GAAGA 7 cut(s) 284, 287, 595, 707, 713, 760, 767
MhlI GDGCHC 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 5 cut(s) 36, 223, 778, 888, 904
MluNI TGGCCA 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 3 cut(s) 194, 305, 854
MmeI TCCRAC 1 cut(s) 72
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 221
MspI CCGG 3 cut(s) 345, 516, 607
MspR9I CCNGG 2 cut(s) 218, 478
Mva1269I GAATGC 1 cut(s) 673
MvaI CCWGG 2 cut(s) 218, 478
MvnI CGCG 4 cut(s) 411, 420, 463, 506
MwoI GCNNNNNNNGC 8 cut(s) 264, 417, 460, 512, 521, 551, 733, 821
NarI GGCGCC 1 cut(s) 171
NdeII GATC 3 cut(s) 447, 471, 615
NlaIII CATG 5 cut(s) 35, 44, 458, 545, 566
NlaIV GGNNCC 1 cut(s) 172
NmeAIII GCCGAG 1 cut(s) 841
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 3 cut(s) 458, 545, 566
PaeI GCATGC 3 cut(s) 458, 545, 566
PasI CCCWGGG 1 cut(s) 477
PcsI WCGNNNNNNNCGW 5 cut(s) 255, 267, 300, 321, 402
PctI GAATGC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 348
PkrI GCNGC 6 cut(s) 321, 460, 491, 540, 592, 823
PleI GAGTC 3 cut(s) 194, 304, 853
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 3 cut(s) 194, 304, 853
Psp6I CCWGG 2 cut(s) 216, 476
PspGI CCWGG 2 cut(s) 216, 476
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 3 cut(s) 233, 536, 788
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 656
SalI GTCGAC 1 cut(s) 395
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 6 cut(s) 320, 459, 490, 539, 591, 822
Sau3AI GATC 3 cut(s) 447, 471, 615
Sau96I GGNCC 3 cut(s) 233, 536, 788
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 3 cut(s) 194, 305, 854
ScrFI CCNGG 2 cut(s) 218, 478
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 4 cut(s) 387, 405, 648, 714
SfcI CTRYAG 1 cut(s) 108
SfoI GGCGCC 1 cut(s) 172
SinI GGWCC 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
SphI GCATGC 3 cut(s) 458, 545, 566
Sse9I AATT 5 cut(s) 36, 223, 778, 888, 904
SsiI CCGC 7 cut(s) 120, 310, 409, 458, 504, 539, 591
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 3 cut(s) 84, 602, 837
StyD4I CCNGG 2 cut(s) 216, 476
StyI CCWWGG 2 cut(s) 352, 441
TaaI ACNGT 3 cut(s) 62, 304, 629
TaiI ACGT 2 cut(s) 264, 429
TaqI TCGA 6 cut(s) 270, 282, 294, 324, 396, 405
TasI AATT 5 cut(s) 36, 223, 778, 888, 904
TatI WGTACW 1 cut(s) 62
TauI GCSGC 3 cut(s) 461, 541, 593
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 260, 442
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 319, 489, 821
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 20, 29
TspRI CASTG 2 cut(s) 260, 442
VneI GTGCAC 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 2 cut(s) 36, 778
XceI RCATGY 3 cut(s) 458, 545, 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XmiI GTMKAC 1 cut(s) 396
XspI CTAG 3 cut(s) 84, 602, 837
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.