MD04G1168400.v1.1

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013031
Physical Location & Seq
Forward (+)
25857382 .. 25858638
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1168400.v1.1.491

Sequence Viewer

Length: 879 bp
ATGCATATGTTTGTTTCTTTCTTCACAAACAGCCCTCTCTTGTTGGATGGAAAAGTTGCTATAGTGACAGGTGGTGCTAGAGGCATTGGAGAAGCAACAGTGAAGCTTTTTGCAAGGCATGGCGCCAAAGTGGTCATTGCTGACGTGGAGGATGCAGTTGGCACAGCCCTAGCAAATTCCCTGAGCCCTTCAGTTACATATGTTCACTGTGATGTTAGCTTGGAAGAAGACATGGAAAACCTAATTGAATCAACAATCACCCGTTACGGGCAACTGGACATTATGTTCAACAATGCCGGGGTCCTCGGAAACCAGTCGAAACACAAGAGCATCATGAACTTCGATGTCGATGAATTTGATAGCGTAATGCGGGTGAATGTTAGAGGAGCTGCATTGGGGATGAAGCATGCTGCAAGAGTGATGGTTGCTAGAGGAATCGGCGGGTGCATAATTTCAACCGCAAGTGTAGCCGGAGTCACAGGCGGGCTGGGGCCACATGGTTACACAGCTTCAAAGCATGCCATTGTAGGGCTCACAAAGAACGCAGCTTGTGAATTGGGAAGGTACGGAATTAGGGTGAATTGCATTTCTCCGTTTGGGGTTGCCACGTCAATGCTGGTCAACGCTTGGAGGATGAGTGGTGGTGATGAAGAAGATGCAGAGGAATGCATGGATTTAGTGATGCCTTGTGAGCAAGAAGTGGAGAAAATGGAAGAGTTTGTGAGAGGGCTTGCAAATTTAAAAGGTCAAACTTTGAGGGCAAAAGACATAGCTGAGGCTGCTCTTTATCTTGCTAGTGATGAATCCAAGTATGTCAGTGGTCATAACTTGGTTGTGGATGGTGGAATCACCACTTCAAGGAATTGTGTTGGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

293

Amino Acids

30.97

Weight (kDa)

5.39

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 18 - 210 4.8e-48 short chain dehydrogenase
KR PF08659 21 - 175 1.2e-10 KR domain
adh_short_C2 PF13561 25 - 210 1.6e-46 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 234 - 284 2.3e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AciI CCGC 4 cut(s) 370, 441, 459, 483
AcsI RAATTY 3 cut(s) 175, 353, 736
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 1 cut(s) 566
AfiI CCNNNNNNNGG 3 cut(s) 267, 528, 858
AgsI TTSAA 5 cut(s) 248, 289, 456, 513, 858
AjiI CACGTC 2 cut(s) 145, 609
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 6 cut(s) 106, 219, 389, 509, 548, 773
AluI AGCT 6 cut(s) 106, 219, 389, 509, 548, 773
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 389, 410, 545, 779
ApoI RAATTY 3 cut(s) 175, 353, 736
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 301, 491
AsuC2I CCSGG 1 cut(s) 298
AsuHPI GGTGA 5 cut(s) 250, 385, 589, 656, 841
AvaII GGWCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 2 cut(s) 188, 534
BbsI GAAGAC 1 cut(s) 234
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 4 cut(s) 376, 397, 557, 766
BccI CCATC 3 cut(s) 41, 415, 833
BcnI CCSGG 1 cut(s) 298
BfaI CTAG 4 cut(s) 78, 170, 429, 795
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 126
BisI GCNGC 4 cut(s) 390, 411, 546, 780
BlsI GCNGC 4 cut(s) 391, 412, 547, 781
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 301
BmgBI CACGTC 2 cut(s) 145, 609
BmgT120I GGNCC 2 cut(s) 301, 491
BmiI GGNNCC 3 cut(s) 124, 302, 492
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 4 cut(s) 142, 339, 646, 672
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 2 cut(s) 182, 774
BpuMI CCSGG 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 297, 304
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 528, 858
Bse1I ACTGG 2 cut(s) 279, 313
Bse3DI GCAATG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 297, 304
BseGI GGATG 5 cut(s) 52, 157, 405, 639, 844
BseLI CCNNNNNNNGG 3 cut(s) 267, 528, 858
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 2 cut(s) 173, 765
BseNI ACTGG 2 cut(s) 279, 313
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 4 cut(s) 376, 397, 557, 766
BseYI CCCAGC 1 cut(s) 487
BshFI GGCC 1 cut(s) 493
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 2 cut(s) 297, 471
BslI CCNNNNNNNGG 3 cut(s) 267, 528, 858
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 1 cut(s) 493
Bsp1286I GDGCHC 2 cut(s) 188, 534
BspACI CCGC 4 cut(s) 370, 441, 459, 483
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 2 cut(s) 174, 766
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 124, 302, 492
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 2 cut(s) 279, 313
BssECI CCNNGG 2 cut(s) 297, 304
BssNI GRCGYC 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 100, 209
Bst6I CTCTTC 1 cut(s) 708
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 4 cut(s) 408, 485, 519, 732
BstDEI CTNAG 2 cut(s) 182, 774
BstF5I GGATG 5 cut(s) 52, 157, 405, 639, 844
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 3 cut(s) 467, 691, 779
BstNSI RCATGY 2 cut(s) 410, 521
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 4 cut(s) 376, 397, 557, 766
BstV2I GAAGAC 1 cut(s) 234
BsuRI GGCC 1 cut(s) 493
BtrI CACGTC 2 cut(s) 145, 609
BtsCI GGATG 5 cut(s) 52, 157, 405, 639, 844
BtsIMutI CAGTG 3 cut(s) 105, 205, 823
Cac8I GCNNGC 4 cut(s) 408, 485, 519, 732
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 301, 491
Csp6I GTAC 1 cut(s) 565
CviAII CATG 7 cut(s) 119, 232, 334, 407, 497, 518, 670
CviQI GTAC 1 cut(s) 565
DdeI CTNAG 2 cut(s) 182, 774
DinI GGCGCC 1 cut(s) 124
DraI TTTAAA 1 cut(s) 741
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Eco24I GRGCYC 2 cut(s) 188, 534
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 301
EcoT22I ATGCAT 2 cut(s) 6, 671
EcoT38I GRGCYC 2 cut(s) 188, 534
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
FaeI CATG 7 cut(s) 122, 235, 337, 410, 500, 521, 673
FatI CATG 7 cut(s) 118, 231, 333, 406, 496, 517, 669
FauI CCCGC 3 cut(s) 363, 434, 476
FauNDI CATATG 2 cut(s) 6, 199
Fnu4HI GCNGC 4 cut(s) 390, 411, 546, 780
FokI GGATG 5 cut(s) 59, 164, 412, 646, 851
FriOI GRGCYC 2 cut(s) 188, 534
Fsp4HI GCNGC 4 cut(s) 390, 411, 546, 780
FspBI CTAG 4 cut(s) 78, 170, 429, 795
GlaI GCGC 1 cut(s) 124
GluI GCNGC 4 cut(s) 390, 411, 546, 780
GsaI CCCAGC 1 cut(s) 491
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 1 cut(s) 493
HapII CCGG 2 cut(s) 297, 471
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 7 cut(s) 122, 235, 337, 410, 500, 521, 673
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 5 cut(s) 248, 435, 474, 803, 846
HpaII CCGG 2 cut(s) 297, 471
HphI GGTGA 5 cut(s) 250, 385, 589, 656, 841
Hpy166II GTNNAC 2 cut(s) 205, 622
Hpy188I TCNGA 1 cut(s) 308
Hpy188III TCNNGA 1 cut(s) 334
Hpy8I GTNNAC 2 cut(s) 205, 622
HpyAV CCTTC 2 cut(s) 198, 555
HpyCH4III ACNGT 2 cut(s) 100, 209
HpyCH4IV ACGT 2 cut(s) 144, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 691, 779
HpyF3I CTNAG 2 cut(s) 182, 774
HpySE526I ACGT 2 cut(s) 144, 608
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 7 cut(s) 122, 235, 337, 410, 500, 521, 673
HspAI GCGC 1 cut(s) 123
KasI GGCGCC 1 cut(s) 122
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 9 cut(s) 54, 194, 260, 310, 326, 465, 473, 484, 602
Lsp1109I GCAGC 4 cut(s) 376, 397, 557, 766
LweI GCATC 4 cut(s) 142, 339, 646, 672
MaeI CTAG 4 cut(s) 78, 170, 429, 795
MaeII ACGT 2 cut(s) 144, 608
MaeIII GTNAC 5 cut(s) 64, 193, 263, 475, 500
MboII GAAGA 6 cut(s) 13, 236, 239, 662, 665, 725
MhlI GDGCHC 2 cut(s) 188, 534
MluCI AATT 9 cut(s) 175, 243, 353, 450, 554, 570, 580, 736, 862
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 1 cut(s) 483
MmeI TCCRAC 1 cut(s) 24
Mph1103I ATGCAT 2 cut(s) 6, 671
MseI TTAA 1 cut(s) 740
MslI CAYNNNNRTG 2 cut(s) 210, 611
MspI CCGG 2 cut(s) 297, 471
MspR9I CCNGG 1 cut(s) 298
Mva1269I GAATGC 1 cut(s) 671
MwoI GCNNNNNNNGC 3 cut(s) 467, 691, 779
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 1 cut(s) 298
NdeI CATATG 2 cut(s) 6, 199
NlaIII CATG 7 cut(s) 122, 235, 337, 410, 500, 521, 673
NlaIV GGNNCC 3 cut(s) 124, 302, 492
NmuCI GTSAC 2 cut(s) 64, 475
NsiI ATGCAT 2 cut(s) 6, 671
NspI RCATGY 2 cut(s) 410, 521
PaeI GCATGC 2 cut(s) 410, 521
PagI TCATGA 1 cut(s) 333
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 4 cut(s) 248, 435, 803, 846
PkrI GCNGC 4 cut(s) 391, 412, 547, 781
PleI GAGTC 1 cut(s) 482
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 487
PspN4I GGNNCC 3 cut(s) 124, 302, 492
PspPI GGNCC 2 cut(s) 301, 491
PspPPI RGGWCCY 1 cut(s) 301
RsaI GTAC 1 cut(s) 566
RsaNI GTAC 1 cut(s) 565
RseI CAYNNNNRTG 2 cut(s) 210, 611
SaqAI TTAA 1 cut(s) 740
SatI GCNGC 4 cut(s) 390, 411, 546, 780
Sau96I GGNCC 2 cut(s) 301, 491
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 2 cut(s) 188, 534
SfaNI GCATC 4 cut(s) 142, 339, 646, 672
SfcI CTRYAG 1 cut(s) 60
SfoI GGCGCC 1 cut(s) 124
SinI GGWCC 1 cut(s) 301
SmiMI CAYNNNNRTG 2 cut(s) 210, 611
SphI GCATGC 2 cut(s) 410, 521
Sse9I AATT 9 cut(s) 175, 243, 353, 450, 554, 570, 580, 736, 862
SsiI CCGC 4 cut(s) 370, 441, 459, 483
SspDI GGCGCC 1 cut(s) 122
SspMI CTAG 4 cut(s) 78, 170, 429, 795
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 100, 209
TaiI ACGT 2 cut(s) 147, 611
TaqI TCGA 3 cut(s) 317, 342, 348
TasI AATT 9 cut(s) 175, 243, 353, 450, 554, 570, 580, 736, 862
TfiI GAWTC 4 cut(s) 248, 435, 803, 846
Tru1I TTAA 1 cut(s) 740
Tru9I TTAA 1 cut(s) 740
TscAI CASTG 3 cut(s) 105, 212, 823
TseFI GTSAC 2 cut(s) 64, 475
TseI GCWGC 4 cut(s) 389, 410, 545, 779
Tsp45I GTSAC 2 cut(s) 64, 475
TspDTI ATGAA 5 cut(s) 350, 366, 416, 663, 816
TspGWI ACGGA 2 cut(s) 582, 582
TspRI CASTG 3 cut(s) 105, 212, 823
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 3 cut(s) 175, 353, 736
XceI RCATGY 2 cut(s) 410, 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XspI CTAG 4 cut(s) 78, 170, 429, 795
Zsp2I ATGCAT 2 cut(s) 6, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.