pycom04g14940

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013031
Physical Location & Seq
Forward (+)
17722516 .. 17723636
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14940.1

Sequence Viewer

Length: 957 bp
ATGCCTGCCCAAGTTATGCCAGAAAAAGCCCTTCATGGAATTCTTGGATTGGGAAGGGACAACATTACTACTACTCCACTTCCTAAAAGGCACTTTCTCCTCGATTCATTTTCTCAATTGTTGGATGGAAAAGTTGCTATTGTGACAGGTGGTGCTAGAGGCATTGGAGAAGCAACAGTGAAGCTTTTTGCAAGGCATGGCGCCAAAGTAGTCATTGCAGACGTGGAGGATGCAGTTGGCACAGCCCTATCAAATTCCCTGAGCCCTTCAGTTACATATGTTCACTGTGATGTTAGCTTGGAAGAAGACATGGAAAACCTAATTGAATCAACAATCACCCGTTACGGGCAACTGGACATTATGTTCAACAATGCTGGGGTCCTCGGAAACCAGTCGAAACACAAGAGCATCATGAACTTTGATGTCGATGAATTTGATAGCATAATGCGGGTGAATGTTAGAGGAGTTGCATTGGGGATGAAGCATGCTGCAAGAGTGATGGTTGCTAGAGGAATCGGCGGGTGCATAATTTCAACCGCAAGTGTAGCCGGAGTCACGGGCGGGCTGGGGCCACATGGTTACACAGCTTCAAAGCATGCCATTGTAGGGCTCACAAAGAACGCAGCTTGTGAATTGGGAAGGTACGGAATTAGGGTAAATTGCATTTCTCCGTTTGGGGTTGCCACGTCAATGCTGGTCAACGCTTGGAGGATGAGTGGTGGTGATGAAGAAGATGAAGAGGAATGCATGGATTTAGTGATGCCTTGTGAGCAAGAAGTGGAGAAAATGGAAGAGTTTGTGAGAGGGCTTGCAAATTTAAAAGGTCAAACTTTGAGGGCAAAAGACATAGCTGAGGCTGCTCTTTATCTTGCTAGTGATGAATCCAAGTATGTTAGTGGTCATAACTTGGTTGTGGATGGTGGAATCACCACTTCAAGAAATTGTGTTGGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

319

Amino Acids

33.85

Weight (kDa)

5.5

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 44 - 236 1e-47 short chain dehydrogenase
KR PF08659 46 - 201 9.3e-10 KR domain
adh_short_C2 PF13561 51 - 236 2.9e-46 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 260 - 310 2.5e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 4 cut(s) 448, 519, 537, 561
AcsI RAATTY 4 cut(s) 39, 253, 431, 814
AcuI CTGAAG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 2 cut(s) 345, 606
AgsI TTSAA 5 cut(s) 326, 367, 534, 591, 936
AjiI CACGTC 2 cut(s) 223, 687
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 5 cut(s) 184, 297, 587, 626, 851
AluI AGCT 5 cut(s) 184, 297, 587, 626, 851
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 3 cut(s) 488, 623, 857
ApoI RAATTY 4 cut(s) 39, 253, 431, 814
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 379, 569
AsuHPI GGTGA 4 cut(s) 328, 463, 734, 919
AvaII GGWCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 2 cut(s) 266, 612
BbsI GAAGAC 1 cut(s) 312
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 3 cut(s) 475, 635, 844
BccI CCATC 3 cut(s) 119, 493, 911
BfaI CTAG 3 cut(s) 156, 507, 873
BfoI RGCGCY 1 cut(s) 204
BisI GCNGC 3 cut(s) 489, 624, 858
BlsI GCNGC 3 cut(s) 490, 625, 859
Bme18I GGWCC 1 cut(s) 379
BmgBI CACGTC 2 cut(s) 223, 687
BmgT120I GGNCC 2 cut(s) 379, 569
BmiI GGNNCC 3 cut(s) 202, 380, 570
BmsI GCATC 3 cut(s) 220, 417, 750
BpiI GAAGAC 1 cut(s) 312
Bpu10I CCTNAGC 2 cut(s) 260, 852
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 382
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 700, 730
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 606
Bse1I ACTGG 2 cut(s) 357, 391
Bse3DI GCAATG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 382
BseGI GGATG 5 cut(s) 130, 235, 483, 717, 922
BseLI CCNNNNNNNGG 2 cut(s) 345, 606
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 251, 843
BseNI ACTGG 2 cut(s) 357, 391
BseRI GAGGAG 2 cut(s) 89, 477
BseXI GCAGC 3 cut(s) 475, 635, 844
BseYI CCCAGC 2 cut(s) 374, 565
BshFI GGCC 1 cut(s) 571
BshNI GGYRCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 345, 606
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 749
BsnI GGCC 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 266, 612
BspACI CCGC 4 cut(s) 448, 519, 537, 561
BspANI GGCC 1 cut(s) 571
BspCNI CTCAG 2 cut(s) 252, 844
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 202, 380, 570
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 213
BsrI ACTGG 2 cut(s) 357, 391
BssECI CCNNGG 1 cut(s) 382
BssNI GRCGYC 1 cut(s) 201
Bst4CI ACNGT 2 cut(s) 178, 287
Bst6I CTCTTC 2 cut(s) 732, 786
BstACI GRCGYC 1 cut(s) 201
BstC8I GCNNGC 5 cut(s) 6, 486, 563, 597, 810
BstDEI CTNAG 2 cut(s) 260, 852
BstF5I GGATG 5 cut(s) 130, 235, 483, 717, 922
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstMWI GCNNNNNNNGC 3 cut(s) 545, 769, 857
BstNSI RCATGY 2 cut(s) 488, 599
BstV1I GCAGC 3 cut(s) 475, 635, 844
BstV2I GAAGAC 1 cut(s) 312
BsuRI GGCC 1 cut(s) 571
BtrI CACGTC 2 cut(s) 223, 687
BtsCI GGATG 5 cut(s) 130, 235, 483, 717, 922
BtsIMutI CAGTG 2 cut(s) 183, 283
Cac8I GCNNGC 5 cut(s) 6, 486, 563, 597, 810
CciI TCATGA 1 cut(s) 411
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 379, 569
Csp6I GTAC 1 cut(s) 643
CviAII CATG 8 cut(s) 35, 197, 310, 412, 485, 575, 596, 748
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 2 cut(s) 260, 852
DinI GGCGCC 1 cut(s) 202
DraI TTTAAA 1 cut(s) 819
Eam1104I CTCTTC 2 cut(s) 732, 786
EarI CTCTTC 2 cut(s) 732, 786
Eco24I GRGCYC 2 cut(s) 266, 612
Eco47I GGWCC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 749
EcoT38I GRGCYC 2 cut(s) 266, 612
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
FaeI CATG 8 cut(s) 38, 200, 313, 415, 488, 578, 599, 751
FaqI GGGAC 1 cut(s) 71
FatI CATG 8 cut(s) 34, 196, 309, 411, 484, 574, 595, 747
FauI CCCGC 3 cut(s) 441, 512, 554
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 489, 624, 858
FokI GGATG 5 cut(s) 137, 242, 490, 724, 929
FriOI GRGCYC 2 cut(s) 266, 612
Fsp4HI GCNGC 3 cut(s) 489, 624, 858
FspBI CTAG 3 cut(s) 156, 507, 873
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 489, 624, 858
GsaI CCCAGC 2 cut(s) 378, 569
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 1 cut(s) 571
HapII CCGG 1 cut(s) 549
HhaI GCGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 8 cut(s) 38, 200, 313, 415, 488, 578, 599, 751
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 6 cut(s) 104, 326, 513, 552, 881, 924
HpaII CCGG 1 cut(s) 549
HphI GGTGA 4 cut(s) 328, 463, 734, 919
Hpy166II GTNNAC 2 cut(s) 283, 700
Hpy188I TCNGA 1 cut(s) 386
Hpy188III TCNNGA 2 cut(s) 412, 936
Hpy8I GTNNAC 2 cut(s) 283, 700
HpyAV CCTTC 4 cut(s) 41, 48, 276, 633
HpyCH4III ACNGT 2 cut(s) 178, 287
HpyCH4IV ACGT 2 cut(s) 222, 686
HpyCH4V TGCA 9 cut(s) 191, 218, 233, 470, 491, 525, 663, 747, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 545, 769, 857
HpyF3I CTNAG 2 cut(s) 260, 852
HpySE526I ACGT 2 cut(s) 222, 686
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 8 cut(s) 38, 200, 313, 415, 488, 578, 599, 751
HspAI GCGC 1 cut(s) 201
KasI GGCGCC 1 cut(s) 200
Lsp1109I GCAGC 3 cut(s) 475, 635, 844
LweI GCATC 3 cut(s) 220, 417, 750
MaeI CTAG 3 cut(s) 156, 507, 873
MaeII ACGT 2 cut(s) 222, 686
MaeIII GTNAC 5 cut(s) 142, 271, 341, 553, 578
MboII GAAGA 6 cut(s) 314, 317, 740, 743, 749, 803
MfeI CAATTG 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 266, 612
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 561
MmeI TCCRAC 1 cut(s) 102
Mph1103I ATGCAT 1 cut(s) 749
MseI TTAA 1 cut(s) 818
MslI CAYNNNNRTG 2 cut(s) 288, 689
MspI CCGG 1 cut(s) 549
MunI CAATTG 1 cut(s) 116
Mva1269I GAATGC 1 cut(s) 749
MwoI GCNNNNNNNGC 3 cut(s) 545, 769, 857
NarI GGCGCC 1 cut(s) 201
NdeI CATATG 1 cut(s) 277
NlaIII CATG 8 cut(s) 38, 200, 313, 415, 488, 578, 599, 751
NlaIV GGNNCC 3 cut(s) 202, 380, 570
NmuCI GTSAC 2 cut(s) 142, 553
NsiI ATGCAT 1 cut(s) 749
NspI RCATGY 2 cut(s) 488, 599
PaeI GCATGC 2 cut(s) 488, 599
PagI TCATGA 1 cut(s) 411
PctI GAATGC 1 cut(s) 749
PfeI GAWTC 5 cut(s) 104, 326, 513, 881, 924
PkrI GCNGC 3 cut(s) 490, 625, 859
PleI GAGTC 1 cut(s) 560
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 560
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
PspFI CCCAGC 2 cut(s) 374, 565
PspN4I GGNNCC 3 cut(s) 202, 380, 570
PspPI GGNCC 2 cut(s) 379, 569
PspPPI RGGWCCY 1 cut(s) 379
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 2 cut(s) 288, 689
SaqAI TTAA 1 cut(s) 818
SatI GCNGC 3 cut(s) 489, 624, 858
Sau96I GGNCC 2 cut(s) 379, 569
SchI GAGTC 1 cut(s) 561
SduI GDGCHC 2 cut(s) 266, 612
SfaNI GCATC 3 cut(s) 220, 417, 750
SfoI GGCGCC 1 cut(s) 202
SinI GGWCC 1 cut(s) 379
SmiMI CAYNNNNRTG 2 cut(s) 288, 689
SphI GCATGC 2 cut(s) 488, 599
SsiI CCGC 4 cut(s) 448, 519, 537, 561
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 3 cut(s) 156, 507, 873
TaaI ACNGT 2 cut(s) 178, 287
TaiI ACGT 2 cut(s) 225, 689
TaqI TCGA 3 cut(s) 102, 395, 426
TfiI GAWTC 5 cut(s) 104, 326, 513, 881, 924
Tru1I TTAA 1 cut(s) 818
Tru9I TTAA 1 cut(s) 818
TscAI CASTG 2 cut(s) 183, 290
TseFI GTSAC 2 cut(s) 142, 553
TseI GCWGC 3 cut(s) 488, 623, 857
Tsp45I GTSAC 2 cut(s) 142, 553
TspDTI ATGAA 8 cut(s) 23, 96, 428, 444, 494, 741, 750, 894
TspGWI ACGGA 2 cut(s) 660, 660
TspRI CASTG 2 cut(s) 183, 290
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 4 cut(s) 39, 253, 431, 814
XceI RCATGY 2 cut(s) 488, 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XspI CTAG 3 cut(s) 156, 507, 873
Zsp2I ATGCAT 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.