Rroxscaffold_6G00418890

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013031
Physical Location & Seq
Reverse (-)
40442834 .. 40444017
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418890.1

Sequence Viewer

Length: 909 bp
ATGCCTGAAACACCTCTTCATGGAATTCATGGATTGGGAAGGGACAACAGTACTACTACTCCTTCACATCCTAGAAGTACCAAAGGAAAAGTTGCTATAGTAACGGGCGGTGCACGAGGCATAGGAGAAGCAACAGCGAGGCTTTTCACAAGGCACGGCGCCAAAGTAGTCATTGCCGATGTGGAGGACTCTATCGGCACAACCCTGGCCAATTCCTTAGGCCCTTCAGTCACCTTCGTTCACTGCGACGTCAGCCTCGAAGAAGACATCGAAAACCTAATCGAGTCCACCGTCTCCCGTTTCGGCCAGCTCGACATTATGTTCAACAACGCCGGAATCCTCGGCAACCAATCCAAACACAAGAGCATCATCAACTTCGACGTCGACGAGTTCGACCGCGTGATGCGCGTGAACGTGAGAGGCACTGCCTTGGGGATCAAGCATGCGGCGCGTGTGATGATCCCCAGGGGAGGAGGCTGCATTATTTCAACCGCGAGCGTCGCCGGAGTCGCTGGTGGGTTAGGGCCGCATGCCTACACAGCTTCCAAGCATGCCATTGTTGGGTTGACGAAGAACGCCGCGTGTGAACTAGGCCGGTATGGGATCAGAGTGAACTGTATTTCGCCTTTTGGAGTTGCCACGCCGATGCTGGTGAATGCGTGGAGGATGAGTGACGATGATGAAGAGGAAGAAGGGTGTATGGATTTTGGGATGCCTTGTGAGCAAGAGGTGGAGAAGATGGAAGATTTCGTGAGAGGGCTTGCGAATTTGAAAGGCCCGACTTTGAGGGCTAAGGACATAGCTGAGGCTGCTCTATATCTTGCTAGCGATGAGTCCAAGTATGTAAGTGGTCATAATCTCGTTGTGGATGGTGGAATTACTACCTCAAGAAATTGTGTTGGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

302

Amino Acids

32.0

Weight (kDa)

5.81

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 30 - 221 8.2e-51 short chain dehydrogenase
KR PF08659 32 - 186 1.6e-11 KR domain
adh_short_C2 PF13561 37 - 221 6.9e-50 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 244 - 294 3.9e-09 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51680
fragaria_vesca FvH4_6g10300
malus_domestica MD04G1168400.v1.1 MD12G1181900.v1.1
prunus_persica Prupe.6G286600_v2.0.a1
pyrus_communis pycom04g14940 pycom12g17110
rosa_chinensis RchiOBHm_Chr3g0461051
rosa_laevigata RLG00000024955
rosa_multiflora Rmu_sc0012073.1_g000001 Rmu_sc0012074.1_g000001
rosa_roxburghii Rroxscaffold_6G00418890
rosa_rugosa Rorug03G0048100
rosa_samantha Rh3AG105400 Rh3BG109400 Rh3CG111000 Rh3DG111100
rosa_wichuraiana Rw3G009030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 252, 384
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 1 cut(s) 384
AccII CGCG 5 cut(s) 399, 408, 451, 494, 581
AciI CCGC 6 cut(s) 108, 397, 446, 492, 527, 579
AclWI GGATC 3 cut(s) 443, 454, 611
AcoI YGGCCR 2 cut(s) 207, 304
AcsI RAATTY 2 cut(s) 24, 766
AcuI CTGAAG 1 cut(s) 210
AcyI GRCGYC 3 cut(s) 159, 249, 381
AfaI GTAC 2 cut(s) 52, 79
AfiI CCNNNNNNNGG 3 cut(s) 20, 470, 561
AgsI TTSAA 3 cut(s) 325, 489, 772
AjnI CCWGG 2 cut(s) 204, 464
AluBI AGCT 3 cut(s) 310, 542, 803
AluI AGCT 3 cut(s) 310, 542, 803
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 298
Alw44I GTGCAC 1 cut(s) 111
AlwI GGATC 3 cut(s) 443, 454, 611
AoxI GGCC 6 cut(s) 207, 220, 304, 524, 592, 775
ApaLI GTGCAC 1 cut(s) 111
ApeKI GCWGC 2 cut(s) 477, 809
ApoI RAATTY 2 cut(s) 24, 766
AspLEI GCGC 3 cut(s) 161, 408, 451
AspS9I GGNCC 3 cut(s) 221, 524, 776
AsuHPI GGTGA 2 cut(s) 223, 664
AsuNHI GCTAGC 1 cut(s) 824
AxyI CCTNAGG 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 115
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 158
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 115
BbvCI CCTCAGC 1 cut(s) 804
BbvI GCAGC 2 cut(s) 464, 796
BccI CCATC 2 cut(s) 733, 863
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 2 cut(s) 206, 466
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 3 cut(s) 72, 590, 825
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 5 cut(s) 447, 478, 527, 579, 810
BlsI GCNGC 5 cut(s) 448, 479, 528, 580, 811
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 206, 466
BmgT120I GGNCC 3 cut(s) 221, 524, 776
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 206, 466
BmsI GCATC 4 cut(s) 375, 393, 636, 702
BmtI GCTAGC 1 cut(s) 828
BpiI GAAGAC 1 cut(s) 270
Bpu10I CCTNAGC 2 cut(s) 792, 804
BpuEI CTTGAG 1 cut(s) 871
BsaHI GRCGYC 3 cut(s) 159, 249, 381
BsaJI CCNNGG 5 cut(s) 204, 340, 429, 464, 465
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 655, 685
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 470, 561
Bse118I RCCGGY 1 cut(s) 594
Bse21I CCTNAGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 2 cut(s) 206, 466
BseDI CCNNGG 5 cut(s) 204, 340, 429, 464, 465
BseGI GGATG 4 cut(s) 67, 672, 717, 874
BseLI CCNNNNNNNGG 3 cut(s) 20, 470, 561
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 795
BseRI GAGGAG 1 cut(s) 486
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 464, 796
Bsh1236I CGCG 5 cut(s) 399, 408, 451, 494, 581
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 6 cut(s) 209, 222, 306, 526, 594, 777
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 3 cut(s) 333, 504, 595
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 3 cut(s) 20, 470, 561
BsmAI GTCTC 1 cut(s) 298
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 6 cut(s) 209, 222, 306, 526, 594, 777
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 3 cut(s) 435, 459, 603
BspACI CCGC 6 cut(s) 108, 397, 446, 492, 527, 579
BspANI GGCC 6 cut(s) 209, 222, 306, 526, 594, 777
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 5 cut(s) 399, 408, 451, 494, 581
BspLI GGNNCC 1 cut(s) 160
BspOI GCTAGC 1 cut(s) 828
BspPI GGATC 3 cut(s) 443, 454, 611
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 594
BssAI RCCGGY 1 cut(s) 594
BssECI CCNNGG 5 cut(s) 204, 340, 429, 464, 465
BssMI GATC 3 cut(s) 435, 459, 603
BssNI GRCGYC 3 cut(s) 159, 249, 381
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 206, 466
Bst4CI ACNGT 3 cut(s) 50, 292, 617
Bst6I CTCTTC 2 cut(s) 21, 678
BstACI GRCGYC 3 cut(s) 159, 249, 381
BstC8I GCNNGC 7 cut(s) 308, 444, 496, 531, 552, 762, 826
BstDEI CTNAG 3 cut(s) 217, 792, 804
BstF5I GGATG 4 cut(s) 67, 672, 717, 874
BstFNI CGCG 5 cut(s) 399, 408, 451, 494, 581
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 3 cut(s) 161, 408, 451
BstKTI GATC 3 cut(s) 438, 462, 606
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 3 cut(s) 435, 459, 603
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 8 cut(s) 252, 405, 448, 500, 509, 539, 721, 809
BstNI CCWGG 2 cut(s) 206, 466
BstNSI RCATGY 3 cut(s) 446, 533, 554
BstSCI CCNGG 2 cut(s) 204, 464
BstSFI CTRYAG 1 cut(s) 96
BstSLI GKGCMC 1 cut(s) 115
BstUI CGCG 5 cut(s) 399, 408, 451, 494, 581
BstV1I GCAGC 2 cut(s) 464, 796
BstV2I GAAGAC 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 217
BsuRI GGCC 6 cut(s) 209, 222, 306, 526, 594, 777
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 4 cut(s) 67, 672, 717, 874
BtsI GCAGTG 2 cut(s) 241, 423
BtsIMutI CAGTG 2 cut(s) 241, 423
Cac8I GCNNGC 7 cut(s) 308, 444, 496, 531, 552, 762, 826
CfoI GCGC 3 cut(s) 161, 408, 451
Cfr10I RCCGGY 1 cut(s) 594
Cfr13I GGNCC 3 cut(s) 221, 524, 776
CseI GACGC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 51, 78
CviAII CATG 5 cut(s) 20, 29, 443, 530, 551
CviQI GTAC 2 cut(s) 51, 78
DdeI CTNAG 3 cut(s) 217, 792, 804
DinI GGCGCC 1 cut(s) 160
DpnI GATC 3 cut(s) 437, 461, 605
DpnII GATC 3 cut(s) 435, 459, 603
EaeI YGGCCR 2 cut(s) 207, 304
Eam1104I CTCTTC 2 cut(s) 21, 678
EarI CTCTTC 2 cut(s) 21, 678
Eco130I CCWWGG 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 210
Eco81I CCTNAGG 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 204, 464
EcoT14I CCWWGG 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 5 cut(s) 23, 32, 446, 533, 554
FaqI GGGAC 1 cut(s) 56
FatI CATG 5 cut(s) 19, 28, 442, 529, 550
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 447, 478, 527, 579, 810
FokI GGATG 4 cut(s) 54, 679, 724, 881
Fsp4HI GCNGC 5 cut(s) 447, 478, 527, 579, 810
FspBI CTAG 3 cut(s) 72, 590, 825
GlaI GCGC 3 cut(s) 160, 407, 450
GluI GCNGC 5 cut(s) 447, 478, 527, 579, 810
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 6 cut(s) 209, 222, 306, 526, 594, 777
HapII CCGG 3 cut(s) 333, 504, 595
HgaI GACGC 1 cut(s) 487
HhaI GCGC 3 cut(s) 161, 408, 451
Hin1I GRCGYC 3 cut(s) 159, 249, 381
Hin1II CATG 5 cut(s) 23, 32, 446, 533, 554
Hin6I GCGC 3 cut(s) 159, 406, 449
HinP1I GCGC 3 cut(s) 159, 406, 449
HincII GTYRAC 2 cut(s) 385, 567
HindII GTYRAC 2 cut(s) 385, 567
HinfI GANTC 5 cut(s) 188, 284, 336, 507, 833
HpaII CCGG 3 cut(s) 333, 504, 595
HphI GGTGA 2 cut(s) 223, 664
Hpy166II GTNNAC 8 cut(s) 113, 241, 288, 385, 412, 567, 587, 613
Hpy188I TCNGA 1 cut(s) 608
Hpy188III TCNNGA 2 cut(s) 751, 888
Hpy8I GTNNAC 8 cut(s) 113, 241, 288, 385, 412, 567, 587, 613
Hpy99I CGWCG 5 cut(s) 251, 383, 386, 389, 503
HpyAV CCTTC 5 cut(s) 33, 72, 234, 244, 686
HpyCH4III ACNGT 3 cut(s) 50, 292, 617
HpyCH4IV ACGT 3 cut(s) 249, 381, 414
HpyCH4V TGCA 2 cut(s) 113, 480
HpyF10VI GCNNNNNNNGC 8 cut(s) 252, 405, 448, 500, 509, 539, 721, 809
HpyF3I CTNAG 3 cut(s) 217, 792, 804
HpySE526I ACGT 3 cut(s) 249, 381, 414
Hsp92I GRCGYC 3 cut(s) 159, 249, 381
Hsp92II CATG 5 cut(s) 23, 32, 446, 533, 554
HspAI GCGC 3 cut(s) 159, 406, 449
KasI GGCGCC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 435, 459, 603
Lsp1109I GCAGC 2 cut(s) 464, 796
LweI GCATC 4 cut(s) 375, 393, 636, 702
MaeI CTAG 3 cut(s) 72, 590, 825
MaeII ACGT 3 cut(s) 249, 381, 414
MaeIII GTNAC 3 cut(s) 100, 229, 671
MalI GATC 3 cut(s) 437, 461, 605
MboI GATC 3 cut(s) 435, 459, 603
MboII GAAGA 8 cut(s) 8, 272, 275, 583, 695, 701, 748, 755
MhlI GDGCHC 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 5 cut(s) 24, 211, 766, 876, 892
MluNI TGGCCA 1 cut(s) 209
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 4 cut(s) 182, 293, 516, 842
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 3 cut(s) 333, 504, 595
MspR9I CCNGG 2 cut(s) 206, 466
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 2 cut(s) 206, 466
MvnI CGCG 5 cut(s) 399, 408, 451, 494, 581
MwoI GCNNNNNNNGC 8 cut(s) 252, 405, 448, 500, 509, 539, 721, 809
NarI GGCGCC 1 cut(s) 159
NdeII GATC 3 cut(s) 435, 459, 603
NheI GCTAGC 1 cut(s) 824
NlaIII CATG 5 cut(s) 23, 32, 446, 533, 554
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 2 cut(s) 229, 671
NspI RCATGY 3 cut(s) 446, 533, 554
PaeI GCATGC 3 cut(s) 446, 533, 554
PasI CCCWGGG 1 cut(s) 465
PcsI WCGNNNNNNNCGW 6 cut(s) 243, 255, 288, 309, 384, 390
PctI GAATGC 1 cut(s) 661
PfeI GAWTC 1 cut(s) 336
PkrI GCNGC 5 cut(s) 448, 479, 528, 580, 811
PleI GAGTC 4 cut(s) 182, 292, 515, 841
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 4 cut(s) 182, 292, 515, 841
Psp6I CCWGG 2 cut(s) 204, 464
PspGI CCWGG 2 cut(s) 204, 464
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 3 cut(s) 221, 524, 776
RsaI GTAC 2 cut(s) 52, 79
RsaNI GTAC 2 cut(s) 51, 78
RseI CAYNNNNRTG 1 cut(s) 644
SalI GTCGAC 1 cut(s) 383
SatI GCNGC 5 cut(s) 447, 478, 527, 579, 810
Sau3AI GATC 3 cut(s) 435, 459, 603
Sau96I GGNCC 3 cut(s) 221, 524, 776
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 4 cut(s) 182, 293, 516, 842
ScrFI CCNGG 2 cut(s) 206, 466
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 4 cut(s) 375, 393, 636, 702
SfcI CTRYAG 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 160
SgrDI CGTCGACG 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
SphI GCATGC 3 cut(s) 446, 533, 554
Sse9I AATT 5 cut(s) 24, 211, 766, 876, 892
SsiI CCGC 6 cut(s) 108, 397, 446, 492, 527, 579
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 3 cut(s) 72, 590, 825
StyD4I CCNGG 2 cut(s) 204, 464
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 3 cut(s) 50, 292, 617
TaiI ACGT 3 cut(s) 252, 384, 417
TaqI TCGA 7 cut(s) 258, 270, 282, 312, 378, 384, 393
TasI AATT 5 cut(s) 24, 211, 766, 876, 892
TatI WGTACW 1 cut(s) 50
TauI GCSGC 3 cut(s) 449, 529, 581
TfiI GAWTC 1 cut(s) 336
TscAI CASTG 2 cut(s) 248, 430
TseFI GTSAC 2 cut(s) 229, 671
TseI GCWGC 2 cut(s) 477, 809
Tsp45I GTSAC 2 cut(s) 229, 671
TspDTI ATGAA 3 cut(s) 8, 17, 696
TspRI CASTG 2 cut(s) 248, 430
VneI GTGCAC 1 cut(s) 111
XapI RAATTY 2 cut(s) 24, 766
XceI RCATGY 3 cut(s) 446, 533, 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 3 cut(s) 72, 590, 825
ZraI GACGTC 2 cut(s) 250, 382
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.