FvH4_6g12130

Steroid 5-alpha-reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014100
Physical Location & Seq
Forward (+)
7324831 .. 7330567
5737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12130.t1

Sequence Viewer

Length: 789 bp
ATGGCCACCTCGTCAGATCAGATCTTCTTCAACAACTGCCTCCTCTGCCTCTACCTCATAGGCCCTCCCACCTTCATCTCCCTCAGATTCCTCCAAGCCCCCTACGGCAAGCACAACCGGCCAGGGTGGGGCCCCACCATCTCTCCGCCGTTGGCTTGGTTCCTCATGGAATCACCAACCCTGTGGCTCACTCTACTCCTCTTCCCCTACGGCCAACACAGCACCAACCCCCAAGCCTTAATTCTAATAACCCTCTTTCTCTTGCACTATATCAACCGAGCCTGCCTCTACCCACTTCGCCTCTACCGGAATACCATCCGGAAAACCGCCCCCGGTTTTCCGGTCAGCGTGGCGTTGATGGCATTTGTGTTCAATTTATTGAATGCTTACTTGCAGTCCAGATGGGTGTCTCACTACAAGGACTATGAGGGTGATCATCATGGGTTGTTTTGGCTGAGGTTTTGTGTTGGGTTGGTTGTGTTTTTGGGTGGGATGAGTGTGAATGTTTGGTCTGATAGGGTTTTAGTGGGGTTGAAAAAAGAGGGCGGAGGGTATAAGATCCCTAGGGGAGGGTGGTTTGAGTTGGTGAGCTGCCCTAACTACTTTGGGGAAATAGTGGAGTGGGTAGGTTGGGCAGTGATGGCTTGGTCTTGGGCAGGACTGGGGTTTTTGCTTTACACGTGCGCTAACTTGGTTCCGAGGGCACGTGCGAACCATAGGTGGTATTTGGAGAAGTTTGGGGAGGATTATCCAAAGTCCAGAAAAGCTGTTATTCCATTCTTGTATTGA

Protein Analysis

263

Amino Acids

30.14

Weight (kDa)

9.39

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 113 - 262 6.3e-54 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38050
fragaria_vesca FvH4_6g12130
malus_domestica MD04G1146500.v1.1 MD12G1159700.v1.1
prunus_persica Prupe.6G270300_v2.0.a1 Prupe.6G270300_v2.0.a1
pyrus_communis pycom12g15140
rosa_chinensis RchiOBHm_Chr3g0463931
rosa_laevigata RLG00000024734
rosa_multiflora Rmu_co8513843.1_g000001 Rmu_sc0006492.1_g000004
rosa_roxburghii Rroxscaffold_6G00416130
rosa_rugosa Rorug03G0069400
rosa_samantha Rh3BG130400 Rh3CG132800 Rh3DG131600
rosa_wichuraiana Rw3G010660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 3 cut(s) 146, 327, 546
AclWI GGATC 1 cut(s) 553
AcoI YGGCCR 3 cut(s) 3, 119, 211
AcvI CACGTG 2 cut(s) 681, 707
AfiI CCNNNNNNNGG 2 cut(s) 128, 569
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 4 cut(s) 31, 373, 382, 535
AjnI CCWGG 1 cut(s) 121
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 2 cut(s) 591, 767
AluI AGCT 2 cut(s) 591, 767
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 553
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 5 cut(s) 3, 61, 119, 130, 211
ApaI GGGCCC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 591
AspA2I CCTAGG 1 cut(s) 563
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 3 cut(s) 62, 130, 131
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 165, 443, 598
AvrII CCTAGG 1 cut(s) 563
BaeGI GKGCMC 2 cut(s) 134, 706
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 134
BbrPI CACGTG 2 cut(s) 681, 707
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 1 cut(s) 578
BccI CCATC 5 cut(s) 146, 323, 352, 396, 634
BceAI ACGGC 3 cut(s) 121, 133, 226
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 1 cut(s) 564
BglII AGATCT 1 cut(s) 21
BisI GCNGC 1 cut(s) 592
BlnI CCTAGG 1 cut(s) 563
BlsI GCNGC 1 cut(s) 593
Bme1390I CCNGG 2 cut(s) 123, 333
BmgT120I GGNCC 3 cut(s) 62, 130, 131
BmiI GGNNCC 5 cut(s) 131, 132, 133, 161, 696
BmrFI CCNGG 2 cut(s) 123, 333
BmrI ACTGGG 1 cut(s) 671
BmuI ACTGGG 1 cut(s) 671
BplI GAGNNNNNCTC 2 cut(s) 270, 302
Bpu10I CCTNAGC 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 333
BsaAI YACGTR 2 cut(s) 681, 707
BsaJI CCNNGG 4 cut(s) 122, 331, 563, 698
BsaWI WCCGGW 3 cut(s) 306, 318, 340
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 569
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 122, 331, 563, 698
BseGI GGATG 2 cut(s) 315, 498
BseLI CCNNNNNNNGG 2 cut(s) 128, 569
BseMII CTCAG 2 cut(s) 97, 446
BseNI ACTGG 1 cut(s) 666
BseRI GAGGAG 2 cut(s) 32, 188
BseSI GKGCMC 2 cut(s) 134, 706
BseXI GCAGC 1 cut(s) 578
BshFI GGCC 5 cut(s) 5, 63, 121, 132, 213
BsiSI CCGG 5 cut(s) 118, 307, 319, 333, 341
BslI CCNNNNNNNGG 2 cut(s) 128, 569
BsmAI GTCTC 1 cut(s) 414
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 5 cut(s) 5, 63, 121, 132, 213
Bsp120I GGGCCC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 134, 706
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 4 cut(s) 16, 21, 433, 558
BspACI CCGC 3 cut(s) 146, 327, 546
BspANI GGCC 5 cut(s) 5, 63, 121, 132, 213
BspCNI CTCAG 2 cut(s) 96, 447
BspEI TCCGGA 1 cut(s) 318
BspLI GGNNCC 5 cut(s) 131, 132, 133, 161, 696
BspPI GGATC 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 1 cut(s) 666
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 4 cut(s) 122, 331, 563, 698
BssMI GATC 4 cut(s) 16, 21, 433, 558
BssT1I CCWWGG 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 206
BstBAI YACGTR 2 cut(s) 681, 707
BstC8I GCNNGC 2 cut(s) 110, 283
BstDEI CTNAG 2 cut(s) 83, 455
BstENI CCTNNNNNAGG 1 cut(s) 567
BstF5I GGATG 2 cut(s) 315, 498
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 4 cut(s) 19, 24, 436, 561
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 4 cut(s) 16, 21, 433, 558
BstMWI GCNNNNNNNGC 5 cut(s) 45, 118, 219, 359, 641
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 2 cut(s) 121, 331
BstSLI GKGCMC 2 cut(s) 134, 706
BstV1I GCAGC 1 cut(s) 578
BstX2I RGATCY 2 cut(s) 21, 558
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 21, 558
BsuRI GGCC 5 cut(s) 5, 63, 121, 132, 213
BtsCI GGATG 2 cut(s) 315, 498
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 2 cut(s) 110, 283
CfoI GCGC 1 cut(s) 686
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 3 cut(s) 62, 130, 131
CviAII CATG 2 cut(s) 166, 440
DdeI CTNAG 2 cut(s) 83, 455
DpnI GATC 4 cut(s) 18, 23, 435, 560
DpnII GATC 4 cut(s) 16, 21, 433, 558
EaeI YGGCCR 3 cut(s) 3, 119, 211
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 2 cut(s) 135, 561
Eco130I CCWWGG 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 134
Eco72I CACGTG 2 cut(s) 681, 707
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoO109I RGGNCCY 3 cut(s) 62, 130, 131
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 563
FaeI CATG 2 cut(s) 169, 443
FaiI YATR 7 cut(s) 59, 167, 270, 426, 441, 555, 717
FatI CATG 2 cut(s) 165, 439
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 1 cut(s) 592
FokI GGATG 2 cut(s) 302, 505
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 592
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 685
GluI GCNGC 1 cut(s) 592
HaeIII GGCC 5 cut(s) 5, 63, 121, 132, 213
HapII CCGG 5 cut(s) 118, 307, 319, 333, 341
HhaI GCGC 1 cut(s) 686
Hin1II CATG 2 cut(s) 169, 443
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HinfI GANTC 2 cut(s) 87, 170
HpaII CCGG 5 cut(s) 118, 307, 319, 333, 341
HphI GGTGA 3 cut(s) 165, 443, 598
Hpy188I TCNGA 5 cut(s) 16, 21, 86, 514, 699
Hpy188III TCNNGA 3 cut(s) 319, 399, 759
HpyAV CCTTC 1 cut(s) 82
HpyCH4IV ACGT 2 cut(s) 680, 706
HpyCH4V TGCA 2 cut(s) 265, 394
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 118, 219, 359, 641
HpyF3I CTNAG 2 cut(s) 83, 455
HpySE526I ACGT 2 cut(s) 680, 706
Hsp92II CATG 2 cut(s) 169, 443
HspAI GCGC 1 cut(s) 684
Kpn2I TCCGGA 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 4 cut(s) 16, 21, 433, 558
Lsp1109I GCAGC 1 cut(s) 578
MaeI CTAG 1 cut(s) 564
MaeII ACGT 2 cut(s) 680, 706
MalI GATC 4 cut(s) 18, 23, 435, 560
MboI GATC 4 cut(s) 16, 21, 433, 558
MboII GAAGA 3 cut(s) 16, 19, 193
MflI RGATCY 2 cut(s) 21, 558
MhlI GDGCHC 2 cut(s) 134, 706
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 240, 373
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 318
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 5 cut(s) 118, 307, 319, 333, 341
MspR9I CCNGG 2 cut(s) 123, 333
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 5 cut(s) 45, 118, 219, 359, 641
NciI CCSGG 1 cut(s) 333
NdeII GATC 4 cut(s) 16, 21, 433, 558
NlaIII CATG 2 cut(s) 169, 443
NlaIV GGNNCC 5 cut(s) 131, 132, 133, 161, 696
PctI GAATGC 1 cut(s) 388
PfeI GAWTC 2 cut(s) 87, 170
PkrI GCNGC 1 cut(s) 593
PmaCI CACGTG 2 cut(s) 681, 707
PmlI CACGTG 2 cut(s) 681, 707
Ppu21I YACGTR 2 cut(s) 681, 707
Psp6I CCWGG 1 cut(s) 121
PspCI CACGTG 2 cut(s) 681, 707
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 5 cut(s) 131, 132, 133, 161, 696
PspOMI GGGCCC 1 cut(s) 130
PspPI GGNCC 3 cut(s) 62, 130, 131
PsuI RGATCY 2 cut(s) 21, 558
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 1 cut(s) 592
Sau3AI GATC 4 cut(s) 16, 21, 433, 558
Sau96I GGNCC 3 cut(s) 62, 130, 131
ScrFI CCNGG 2 cut(s) 123, 333
SduI GDGCHC 2 cut(s) 134, 706
Sse9I AATT 2 cut(s) 240, 373
SsiI CCGC 3 cut(s) 146, 327, 546
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 121, 331
StyI CCWWGG 1 cut(s) 563
TaiI ACGT 2 cut(s) 683, 709
TasI AATT 2 cut(s) 240, 373
TfiI GAWTC 2 cut(s) 87, 170
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TscAI CASTG 1 cut(s) 642
TseI GCWGC 1 cut(s) 591
TspDTI ATGAA 1 cut(s) 64
TspRI CASTG 1 cut(s) 642
XagI CCTNNNNNAGG 1 cut(s) 567
XmaJI CCTAGG 1 cut(s) 563
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.