Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014100

Physical Location & Seq

Genomic Coordinates Reverse (-)

23492564 .. 23493349

Gene Structure 786 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1146500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 786 bp

ATGGCATTGTCAGATCAGACCTTCTTCCACAACTGCCTTATAGCTCTCTACCTCATAGCTCCCCCAACCTTCATCTCCCTCCGGTTCCTCCAAGCCCCATACGGCAAGCACCGCCGCCCGGGGTGGGGCCCCACAATGTCCCCGCCCCTCGCCTGGTTCCTCATGGAATCCCCAACTCTCTGGCTAACCCTCCTCCTCTTCCCCTCCGGCCGCCACTCCTCCAACCCAAAAGCCCTTTTGCTCATCACCCCCTTTCTCTTCCACTATTTCCACCGCACCTGCCTCTACCCGCTCCGCCTCCACCGCGACACCGCCAGTTCCAAATCCTCCGCCGGCGGTTTTCCGGTGAGCGTGGCGGCGATGGCGTTTGGTTTCAACCTGTTGAATGCGTATCTGCAGGGCAGGTGGATTTCTGAGTACAAAGATTATGAATCTGACGGGTGGTTTTGGTGGCGGTTTATGGGTGGGTTGGCGGTGTTTGGTTATGGCATGGGTGTAAATATATGGTCTGATAGCGTCCTCGTGGGGTTGAAAAAAGAGGGTGGGGGGTATAAAGTGCCAAAAGGAGGGTGGTTTGAGTTGGTGAGCTGCCCAAACTACTATGGGGAGATAGTGGAGTGGGCAGGATGGGCAGTGATGACGTGGTCCTGGGTAGGGCTGGGGTTTTTTCTTTACACGTGTGCAAACTTGGTGCCTAGGGCACGTGCGAATCACCGGTGGTATTTAGAGAAGTTTGGAGAGGATTATCCCAAGGGTAGAAAAGCTGTCCTCCCATTCTTGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0006629 GO:0006694 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009917 GO:0010268 GO:0010817 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016229 GO:0016491 GO:0016627 GO:0016628 GO:0033765 GO:0042445 GO:0042446 GO:0042592 GO:0044238 GO:0048878 GO:0050213 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00905 ko01100 ko01110 map00905 map01100 map01110

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

262

Amino Acids

29.87

Weight (kDa)

9.39

Isoelectric Point (pI)

50.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	114 - 261	7.3e-50	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38050
fragaria_vesca	FvH4_6g12130
malus_domestica	MD04G1146500.v1.1 MD12G1159700.v1.1
prunus_persica	Prupe.6G270300_v2.0.a1 Prupe.6G270300_v2.0.a1
pyrus_communis	pycom12g15140
rosa_chinensis	RchiOBHm_Chr3g0463931
rosa_laevigata	RLG00000024734
rosa_multiflora	Rmu_co8513843.1_g000001 Rmu_sc0006492.1_g000004
rosa_roxburghii	Rroxscaffold_6G00416130
rosa_rugosa	Rorug03G0069400
rosa_samantha	Rh3BG130400 Rh3CG132800 Rh3DG131600
rosa_wichuraiana	Rw3G010660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	287, 393
Acc36I	ACCTGC	2 cut(s)	287, 393
AccB1I	GGYRCC	1 cut(s)	691
AccBSI	CCGCTC	1 cut(s)	292
AccII	CGCG	1 cut(s)	306
AcoI	YGGCCR	1 cut(s)	208
AcvI	CACGTG	2 cut(s)	678, 704
AfaI	GTAC	1 cut(s)	419
AfiI	CCNNNNNNNGG	6 cut(s)	118, 124, 125, 153, 566, 654
AflIII	ACRYGT	2 cut(s)	675, 677
AgeI	ACCGGT	1 cut(s)	714
AgsI	TTSAA	3 cut(s)	376, 385, 532
AjiI	CACGTC	1 cut(s)	642
AjnI	CCWGG	2 cut(s)	152, 647
AluBI	AGCT	4 cut(s)	44, 59, 588, 764
AluI	AGCT	4 cut(s)	44, 59, 588, 764
Ama87I	CYCGRG	1 cut(s)	118
AoxI	GGCC	2 cut(s)	127, 208
ApaI	GGGCCC	1 cut(s)	131
ApeKI	GCWGC	1 cut(s)	588
AsiGI	ACCGGT	1 cut(s)	714
AspA2I	CCTAGG	1 cut(s)	695
AspS9I	GGNCC	3 cut(s)	127, 128, 645
AsuC2I	CCSGG	2 cut(s)	119, 120
AsuHPI	GGTGA	4 cut(s)	238, 358, 595, 704
AvaI	CYCGRG	1 cut(s)	118
AvaII	GGWCC	1 cut(s)	645
AvrII	CCTAGG	1 cut(s)	695
BaeGI	GKGCMC	2 cut(s)	131, 703
BanI	GGYRCC	1 cut(s)	691
BanII	GRGCYC	1 cut(s)	131
BauI	CACGAG	1 cut(s)	521
BbrPI	CACGTG	2 cut(s)	678, 704
BbvI	GCAGC	1 cut(s)	575
BccI	CCATC	2 cut(s)	355, 621
BceAI	ACGGC	1 cut(s)	118
BciT130I	CCWGG	2 cut(s)	154, 649
BcnI	CCSGG	2 cut(s)	119, 120
BfaI	CTAG	1 cut(s)	696
BfmI	CTRYAG	1 cut(s)	395
BfuAI	ACCTGC	2 cut(s)	287, 393
BisI	GCNGC	4 cut(s)	115, 211, 357, 589
BlnI	CCTAGG	1 cut(s)	695
BlsI	GCNGC	4 cut(s)	116, 212, 358, 590
Bme1390I	CCNGG	4 cut(s)	119, 120, 154, 649
Bme18I	GGWCC	1 cut(s)	645
BmeT110I	CYCGRG	1 cut(s)	118
BmgBI	CACGTC	1 cut(s)	642
BmgT120I	GGNCC	3 cut(s)	127, 128, 645
BmiI	GGNNCC	6 cut(s)	86, 128, 129, 130, 158, 693
BmrFI	CCNGG	4 cut(s)	119, 120, 154, 649
BpuMI	CCSGG	2 cut(s)	119, 120
BsaAI	YACGTR	2 cut(s)	678, 704
BsaJI	CCNNGG	5 cut(s)	118, 119, 648, 695, 750
BsaWI	WCCGGW	3 cut(s)	81, 343, 714
Bsc4I	CCNNNNNNNGG	6 cut(s)	118, 124, 125, 153, 566, 654
Bse118I	RCCGGY	2 cut(s)	332, 714
Bse1I	ACTGG	1 cut(s)	315
BseBI	CCWGG	2 cut(s)	154, 649
BseDI	CCNNGG	5 cut(s)	118, 119, 648, 695, 750
BseGI	GGATG	1 cut(s)	632
BseLI	CCNNNNNNNGG	6 cut(s)	118, 124, 125, 153, 566, 654
BseMII	CTCAG	1 cut(s)	405
BseNI	ACTGG	1 cut(s)	315
BseRI	GAGGAG	3 cut(s)	182, 185, 208
BseSI	GKGCMC	2 cut(s)	131, 703
BseX3I	CGGCCG	1 cut(s)	208
BseXI	GCAGC	1 cut(s)	575
BseYI	CCCAGC	1 cut(s)	658
Bsh1236I	CGCG	1 cut(s)	306
Bsh1285I	CGRYCG	1 cut(s)	211
BshFI	GGCC	2 cut(s)	129, 210
BshNI	GGYRCC	1 cut(s)	691
BshTI	ACCGGT	1 cut(s)	714
BsiEI	CGRYCG	1 cut(s)	211
BsiHKCI	CYCGRG	1 cut(s)	118
BsiSI	CCGG	6 cut(s)	82, 119, 207, 333, 344, 715
BslFI	GGGAC	1 cut(s)	124
BslI	CCNNNNNNNGG	6 cut(s)	118, 124, 125, 153, 566, 654
BsmFI	GGGAC	1 cut(s)	124
BsmI	GAATGC	1 cut(s)	391
BsnI	GGCC	2 cut(s)	129, 210
BsoBI	CYCGRG	1 cut(s)	118
Bsp120I	GGGCCC	1 cut(s)	127
Bsp1286I	GDGCHC	2 cut(s)	131, 703
Bsp143I	GATC	1 cut(s)	13
BspANI	GGCC	2 cut(s)	129, 210
BspCNI	CTCAG	1 cut(s)	406
BspFNI	CGCG	1 cut(s)	306
BspLI	GGNNCC	6 cut(s)	86, 128, 129, 130, 158, 693
BspMAI	CTGCAG	1 cut(s)	399
BspMI	ACCTGC	2 cut(s)	287, 393
BspT107I	GGYRCC	1 cut(s)	691
BsrBI	CCGCTC	1 cut(s)	292
BsrFI	RCCGGY	2 cut(s)	332, 714
BsrI	ACTGG	1 cut(s)	315
BssAI	RCCGGY	2 cut(s)	332, 714
BssECI	CCNNGG	5 cut(s)	118, 119, 648, 695, 750
BssMI	GATC	1 cut(s)	13
BssSI	CACGAG	1 cut(s)	521
BssT1I	CCWWGG	2 cut(s)	695, 750
Bst2BI	CACGAG	1 cut(s)	521
Bst2UI	CCWGG	2 cut(s)	154, 649
Bst6I	CTCTTC	2 cut(s)	203, 263
BstBAI	YACGTR	2 cut(s)	678, 704
BstC8I	GCNNGC	2 cut(s)	107, 334
BstDEI	CTNAG	1 cut(s)	414
BstF5I	GGATG	1 cut(s)	632
BstFNI	CGCG	1 cut(s)	306
BstKTI	GATC	1 cut(s)	16
BstMBI	GATC	1 cut(s)	13
BstMCI	CGRYCG	1 cut(s)	211
BstMWI	GCNNNNNNNGC	4 cut(s)	111, 303, 362, 629
BstNI	CCWGG	2 cut(s)	154, 649
BstSCI	CCNGG	4 cut(s)	117, 118, 152, 647
BstSFI	CTRYAG	1 cut(s)	395
BstSLI	GKGCMC	2 cut(s)	131, 703
BstUI	CGCG	1 cut(s)	306
BstV1I	GCAGC	1 cut(s)	575
BstXI	CCANNNNNNTGG	1 cut(s)	180
BstZI	CGGCCG	1 cut(s)	208
BsuRI	GGCC	2 cut(s)	129, 210
BtgZI	GCGATG	1 cut(s)	374
BtrI	CACGTC	1 cut(s)	642
BtsCI	GGATG	1 cut(s)	632
BtsI	GCAGTG	1 cut(s)	639
BtsIMutI	CAGTG	1 cut(s)	639
BveI	ACCTGC	2 cut(s)	287, 393
Cac8I	GCNNGC	2 cut(s)	107, 334
Cfr10I	RCCGGY	2 cut(s)	332, 714
Cfr13I	GGNCC	3 cut(s)	127, 128, 645
Cfr9I	CCCGGG	1 cut(s)	118
CseI	GACGC	1 cut(s)	505
Csp6I	GTAC	1 cut(s)	418
CspAI	ACCGGT	1 cut(s)	714
CviAII	CATG	2 cut(s)	163, 490
CviQI	GTAC	1 cut(s)	418
DdeI	CTNAG	1 cut(s)	414
DpnI	GATC	1 cut(s)	15
DpnII	GATC	1 cut(s)	13
EaeI	YGGCCR	1 cut(s)	208
EagI	CGGCCG	1 cut(s)	208
Eam1104I	CTCTTC	2 cut(s)	203, 263
EarI	CTCTTC	2 cut(s)	203, 263
EciI	GGCGGA	2 cut(s)	284, 319
EclXI	CGGCCG	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	695, 750
Eco24I	GRGCYC	1 cut(s)	131
Eco47I	GGWCC	1 cut(s)	645
Eco52I	CGGCCG	1 cut(s)	208
Eco72I	CACGTG	2 cut(s)	678, 704
Eco88I	CYCGRG	1 cut(s)	118
EcoO109I	RGGNCCY	2 cut(s)	127, 128
EcoRII	CCWGG	2 cut(s)	152, 647
EcoT14I	CCWWGG	2 cut(s)	695, 750
EcoT38I	GRGCYC	1 cut(s)	131
ErhI	CCWWGG	2 cut(s)	695, 750
FaeI	CATG	2 cut(s)	166, 493
FaqI	GGGAC	1 cut(s)	124
FatI	CATG	2 cut(s)	162, 489
FauI	CCCGC	2 cut(s)	150, 297
Fnu4HI	GCNGC	4 cut(s)	115, 211, 357, 589
FokI	GGATG	1 cut(s)	639
FriOI	GRGCYC	1 cut(s)	131
Fsp4HI	GCNGC	4 cut(s)	115, 211, 357, 589
FspBI	CTAG	1 cut(s)	696
GluI	GCNGC	4 cut(s)	115, 211, 357, 589
GsaI	CCCAGC	1 cut(s)	662
HaeIII	GGCC	2 cut(s)	129, 210
HapII	CCGG	6 cut(s)	82, 119, 207, 333, 344, 715
HgaI	GACGC	1 cut(s)	505
Hin1II	CATG	2 cut(s)	166, 493
HinfI	GANTC	3 cut(s)	167, 431, 709
HpaII	CCGG	6 cut(s)	82, 119, 207, 333, 344, 715
HphI	GGTGA	4 cut(s)	238, 358, 595, 704
Hpy188I	TCNGA	5 cut(s)	13, 18, 415, 436, 511
HpyAV	CCTTC	2 cut(s)	31, 79
HpyCH4IV	ACGT	3 cut(s)	641, 677, 703
HpyCH4V	TGCA	2 cut(s)	397, 683
HpyF10VI	GCNNNNNNNGC	4 cut(s)	111, 303, 362, 629
HpyF3I	CTNAG	1 cut(s)	414
HpySE526I	ACGT	3 cut(s)	641, 677, 703
Hsp92II	CATG	2 cut(s)	166, 493
KroI	GCCGGC	1 cut(s)	332
KroNI	GCCGGC	1 cut(s)	334
Kzo9I	GATC	1 cut(s)	13
LmnI	GCTCC	2 cut(s)	64, 297
Lsp1109I	GCAGC	1 cut(s)	575
MaeI	CTAG	1 cut(s)	696
MaeII	ACGT	3 cut(s)	641, 677, 703
MalI	GATC	1 cut(s)	15
MbiI	CCGCTC	1 cut(s)	292
MboI	GATC	1 cut(s)	13
MboII	GAAGA	3 cut(s)	16, 190, 250
MhlI	GDGCHC	2 cut(s)	131, 703
MmeI	TCCRAC	1 cut(s)	246
MreI	CGCCGGCG	1 cut(s)	332
MroNI	GCCGGC	1 cut(s)	332
MspI	CCGG	6 cut(s)	82, 119, 207, 333, 344, 715
MspR9I	CCNGG	4 cut(s)	119, 120, 154, 649
Mva1269I	GAATGC	1 cut(s)	391
MvaI	CCWGG	2 cut(s)	154, 649
MvnI	CGCG	1 cut(s)	306
MwoI	GCNNNNNNNGC	4 cut(s)	111, 303, 362, 629
NaeI	GCCGGC	1 cut(s)	334
NciI	CCSGG	2 cut(s)	119, 120
NdeII	GATC	1 cut(s)	13
NgoMIV	GCCGGC	1 cut(s)	332
NlaIII	CATG	2 cut(s)	166, 493
NlaIV	GGNNCC	6 cut(s)	86, 128, 129, 130, 158, 693
PaqCI	CACCTGC	2 cut(s)	287, 393
PctI	GAATGC	1 cut(s)	391
PdiI	GCCGGC	1 cut(s)	334
PfeI	GAWTC	3 cut(s)	167, 431, 709
PflFI	GACNNNGTC	1 cut(s)	643
PinAI	ACCGGT	1 cut(s)	714
PkrI	GCNGC	4 cut(s)	116, 212, 358, 590
PmaCI	CACGTG	2 cut(s)	678, 704
PmlI	CACGTG	2 cut(s)	678, 704
Ppu21I	YACGTR	2 cut(s)	678, 704
Psp6I	CCWGG	2 cut(s)	152, 647
PspCI	CACGTG	2 cut(s)	678, 704
PspFI	CCCAGC	1 cut(s)	658
PspGI	CCWGG	2 cut(s)	152, 647
PspN4I	GGNNCC	6 cut(s)	86, 128, 129, 130, 158, 693
PspOMI	GGGCCC	1 cut(s)	127
PspPI	GGNCC	3 cut(s)	127, 128, 645
PstI	CTGCAG	1 cut(s)	399
PsyI	GACNNNGTC	1 cut(s)	643
RsaI	GTAC	1 cut(s)	419
RsaNI	GTAC	1 cut(s)	418
SatI	GCNGC	4 cut(s)	115, 211, 357, 589
Sau3AI	GATC	1 cut(s)	13
Sau96I	GGNCC	3 cut(s)	127, 128, 645
ScrFI	CCNGG	4 cut(s)	119, 120, 154, 649
SduI	GDGCHC	2 cut(s)	131, 703
SfcI	CTRYAG	1 cut(s)	395
SgrAI	CRCCGGYG	2 cut(s)	332, 714
SinI	GGWCC	1 cut(s)	645
SmaI	CCCGGG	1 cut(s)	120
SspMI	CTAG	1 cut(s)	696
StyD4I	CCNGG	4 cut(s)	117, 118, 152, 647
StyI	CCWWGG	2 cut(s)	695, 750
TaiI	ACGT	3 cut(s)	644, 680, 706
TatI	WGTACW	1 cut(s)	417
TauI	GCSGC	3 cut(s)	117, 213, 359
TfiI	GAWTC	3 cut(s)	167, 431, 709
TscAI	CASTG	1 cut(s)	639
TseI	GCWGC	1 cut(s)	588
TspDTI	ATGAA	2 cut(s)	61, 444
TspMI	CCCGGG	1 cut(s)	118
TspRI	CASTG	1 cut(s)	639
Tth111I	GACNNNGTC	1 cut(s)	643
VpaK11BI	GGWCC	1 cut(s)	645
XcmI	CCANNNNNNNNNTGG	1 cut(s)	567
XmaI	CCCGGG	1 cut(s)	118
XmaJI	CCTAGG	1 cut(s)	695
XspI	CTAG	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.