Rh3DG131600

Steroid 5-alpha-reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0014100
Physical Location & Seq
Forward (+)
11229193 .. 11230987
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG131600.1

Sequence Viewer

Length: 945 bp
ATGTGTCTGCCACCAAATGAAGGACAGATAATAGCACAAGAATCCAATTCAGAAATCCAAGCCAATAAGGCAATAACAATTTCCTTTCCCATTCACCATCCCCCCATTACCCATCAACCCCATTCCCCTTTCTATTTCATTCTTCATTCCACACACACAGTCATGGCCTCATCGGATCAGACCTTCTTCAACAACTCCCTCCTAGCTCTCTACCTCATAGGCCCTCCCACATTCATCTCCCTCAGATTCCTCCAAGCCCCATATGGCAAGCACAACCGTCCAGGGTGGGGCCCCACCATGTCTCCGCCGCTGGCTTGGTTCCTCATGGAATCACCAACCCTTGGGCTCACTCTACTCCTCTTCCCCTATGGCCAACACAGTTCCAACCCCCAAGCCTTAATTCTAATGACCCCCTTTCTTTTCCACTATTTCAACCGAACCTGCCTCTACCCACTTCGCCTCTACCGGAATACCACCCGGAAAACCACCCCCGGTTTTCCGGTCAGCGTGGCGTCGATGGCATTTGTGTTCAATCTGTTGAATGGTTATTTGCAATCCAGATGGGTGTCTCACTACAAGGACTATGAGAGTGAAAATGGGTGGTTTTGGTGTAGGTTTTTTGTTGGGTTGGTTGTGTTTTTGTGTGGGATGGGTGTGAATGTGTGGTCTGATAGGGTTTTAGTGGGTTTGAAAACAGAGGGTGGAGGGTATAAGATCCCCAAAGGGGGGTGGTTTGAGTTGGTGAGCTGCCCTAACTACTTTGGGGAAATAGTGGAGTGGATTGGATGGGCAGTGATGACTTGGTCATCAGCAGGACTAGGGTTTATGCTATACACGTGTGCCAATTTGGTGCCCAGGGCACGTGCGAACCATAGTTGGTATTTGGAGAAGTTTGGGGAGGACTATCCCAAGTCCAGAAAAGCTGTTATCCCTTTCTTGTATTGA

Protein Analysis

314

Amino Acids

35.83

Weight (kDa)

8.96

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 166 - 314 3.5e-55 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38050
fragaria_vesca FvH4_6g12130
malus_domestica MD04G1146500.v1.1 MD12G1159700.v1.1
prunus_persica Prupe.6G270300_v2.0.a1 Prupe.6G270300_v2.0.a1
pyrus_communis pycom12g15140
rosa_chinensis RchiOBHm_Chr3g0463931
rosa_laevigata RLG00000024734
rosa_multiflora Rmu_co8513843.1_g000001 Rmu_sc0006492.1_g000004
rosa_roxburghii Rroxscaffold_6G00416130
rosa_rugosa Rorug03G0069400
rosa_samantha Rh3BG130400 Rh3CG132800 Rh3DG131600
rosa_wichuraiana Rw3G010660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 850
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 2 cut(s) 305, 308
AclWI GGATC 2 cut(s) 183, 709
AcoI YGGCCR 1 cut(s) 370
AcvI CACGTG 2 cut(s) 837, 863
AcyI GRCGYC 1 cut(s) 512
AfiI CCNNNNNNNGG 6 cut(s) 20, 287, 341, 724, 725, 726
AflIII ACRYGT 2 cut(s) 834, 836
AgsI TTSAA 5 cut(s) 190, 433, 532, 541, 691
AjnI CCWGG 2 cut(s) 280, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AluBI AGCT 3 cut(s) 206, 747, 923
AluI AGCT 3 cut(s) 206, 747, 923
Alw26I GTCTC 2 cut(s) 306, 573
AlwI GGATC 2 cut(s) 183, 709
AoxI GGCC 4 cut(s) 165, 220, 289, 370
ApaI GGGCCC 1 cut(s) 293
ApeKI GCWGC 1 cut(s) 747
AspS9I GGNCC 3 cut(s) 221, 289, 290
AsuC2I CCSGG 2 cut(s) 478, 492
AsuHPI GGTGA 3 cut(s) 86, 324, 754
BaeGI GKGCMC 3 cut(s) 293, 855, 862
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 2 cut(s) 293, 348
BbrPI CACGTG 2 cut(s) 837, 863
BbvI GCAGC 1 cut(s) 734
BccI CCATC 6 cut(s) 105, 120, 511, 555, 643, 780
BciT130I CCWGG 2 cut(s) 282, 856
BcnI CCSGG 2 cut(s) 478, 492
BcoDI GTCTC 2 cut(s) 306, 573
BfaI CTAG 2 cut(s) 203, 818
BfuAI ACCTGC 1 cut(s) 449
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 2 cut(s) 308, 748
BlsI GCNGC 2 cut(s) 309, 749
Bme1390I CCNGG 4 cut(s) 282, 478, 492, 856
BmgT120I GGNCC 3 cut(s) 221, 289, 290
BmiI GGNNCC 5 cut(s) 290, 291, 292, 320, 852
BmrFI CCNGG 4 cut(s) 282, 478, 492, 856
BpuMI CCSGG 2 cut(s) 478, 492
BsaAI YACGTR 2 cut(s) 837, 863
BsaHI GRCGYC 1 cut(s) 512
BsaJI CCNNGG 5 cut(s) 281, 340, 490, 854, 855
BsaWI WCCGGW 2 cut(s) 465, 499
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 287, 341, 724, 725, 726
BseBI CCWGG 2 cut(s) 282, 856
BseDI CCNNGG 5 cut(s) 281, 340, 490, 854, 855
BseGI GGATG 3 cut(s) 97, 654, 791
BseLI CCNNNNNNNGG 6 cut(s) 20, 287, 341, 724, 725, 726
BseMII CTCAG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 3 cut(s) 293, 855, 862
BseXI GCAGC 1 cut(s) 734
BshFI GGCC 4 cut(s) 167, 222, 291, 372
BshNI GGYRCC 1 cut(s) 850
BsiSI CCGG 4 cut(s) 466, 478, 492, 500
BslI CCNNNNNNNGG 6 cut(s) 20, 287, 341, 724, 725, 726
BsmAI GTCTC 2 cut(s) 306, 573
BsnI GGCC 4 cut(s) 167, 222, 291, 372
Bsp120I GGGCCC 1 cut(s) 289
Bsp1286I GDGCHC 4 cut(s) 293, 348, 855, 862
Bsp143I GATC 2 cut(s) 175, 714
BspACI CCGC 2 cut(s) 305, 308
BspANI GGCC 4 cut(s) 167, 222, 291, 372
BspCNI CTCAG 1 cut(s) 255
BspLI GGNNCC 5 cut(s) 290, 291, 292, 320, 852
BspMI ACCTGC 1 cut(s) 449
BspPI GGATC 2 cut(s) 183, 709
BspT107I GGYRCC 1 cut(s) 850
BssECI CCNNGG 5 cut(s) 281, 340, 490, 854, 855
BssMI GATC 2 cut(s) 175, 714
BssNI GRCGYC 1 cut(s) 512
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 2 cut(s) 282, 856
Bst4CI ACNGT 3 cut(s) 160, 278, 380
Bst6I CTCTTC 1 cut(s) 365
BstACI GRCGYC 1 cut(s) 512
BstBAI YACGTR 2 cut(s) 837, 863
BstC8I GCNNGC 2 cut(s) 269, 312
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 97, 654, 791
BstKTI GATC 2 cut(s) 178, 717
BstMAI GTCTC 2 cut(s) 306, 573
BstMBI GATC 2 cut(s) 175, 714
BstMWI GCNNNNNNNGC 2 cut(s) 68, 518
BstNI CCWGG 2 cut(s) 282, 856
BstSCI CCNGG 4 cut(s) 280, 476, 490, 854
BstSLI GKGCMC 3 cut(s) 293, 855, 862
BstV1I GCAGC 1 cut(s) 734
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 4 cut(s) 167, 222, 291, 372
BtsCI GGATG 3 cut(s) 97, 654, 791
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 1 cut(s) 798
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 2 cut(s) 269, 312
Cfr13I GGNCC 3 cut(s) 221, 289, 290
CseI GACGC 1 cut(s) 501
CviAII CATG 3 cut(s) 163, 298, 325
DdeI CTNAG 1 cut(s) 242
DpnI GATC 2 cut(s) 177, 716
DpnII GATC 2 cut(s) 175, 714
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
EciI GGCGGA 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 2 cut(s) 293, 348
Eco72I CACGTG 2 cut(s) 837, 863
EcoO109I RGGNCCY 3 cut(s) 221, 289, 290
EcoRII CCWGG 2 cut(s) 280, 854
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 2 cut(s) 293, 348
ErhI CCWWGG 1 cut(s) 340
FaeI CATG 3 cut(s) 166, 301, 328
FatI CATG 3 cut(s) 162, 297, 324
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 308, 748
FokI GGATG 3 cut(s) 84, 661, 798
FriOI GRGCYC 2 cut(s) 293, 348
Fsp4HI GCNGC 2 cut(s) 308, 748
FspBI CTAG 2 cut(s) 203, 818
GluI GCNGC 2 cut(s) 308, 748
HaeIII GGCC 4 cut(s) 167, 222, 291, 372
HapII CCGG 4 cut(s) 466, 478, 492, 500
HgaI GACGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 3 cut(s) 166, 301, 328
HinfI GANTC 3 cut(s) 41, 246, 329
HpaII CCGG 4 cut(s) 466, 478, 492, 500
HphI GGTGA 3 cut(s) 86, 324, 754
Hpy188I TCNGA 5 cut(s) 52, 175, 180, 245, 670
Hpy188III TCNNGA 2 cut(s) 558, 915
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 2 cut(s) 14, 193
HpyCH4III ACNGT 3 cut(s) 160, 278, 380
HpyCH4IV ACGT 2 cut(s) 836, 862
HpyCH4V TGCA 1 cut(s) 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 518
HpyF3I CTNAG 1 cut(s) 242
HpySE526I ACGT 2 cut(s) 836, 862
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 3 cut(s) 166, 301, 328
Kzo9I GATC 2 cut(s) 175, 714
Lsp1109I GCAGC 1 cut(s) 734
MaeI CTAG 2 cut(s) 203, 818
MaeII ACGT 2 cut(s) 836, 862
MalI GATC 2 cut(s) 177, 716
MboI GATC 2 cut(s) 175, 714
MboII GAAGA 3 cut(s) 134, 178, 352
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 4 cut(s) 293, 348, 855, 862
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 4 cut(s) 46, 78, 399, 844
MluNI TGGCCA 1 cut(s) 372
MmeI TCCRAC 1 cut(s) 408
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 1 cut(s) 161
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 4 cut(s) 466, 478, 492, 500
MspR9I CCNGG 4 cut(s) 282, 478, 492, 856
MvaI CCWGG 2 cut(s) 282, 856
MwoI GCNNNNNNNGC 2 cut(s) 68, 518
NciI CCSGG 2 cut(s) 478, 492
NdeI CATATG 1 cut(s) 262
NdeII GATC 2 cut(s) 175, 714
NlaIII CATG 3 cut(s) 166, 301, 328
NlaIV GGNNCC 5 cut(s) 290, 291, 292, 320, 852
PasI CCCWGGG 1 cut(s) 855
PfeI GAWTC 3 cut(s) 41, 246, 329
PflFI GACNNNGTC 1 cut(s) 802
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 2 cut(s) 309, 749
PmaCI CACGTG 2 cut(s) 837, 863
PmlI CACGTG 2 cut(s) 837, 863
Ppu21I YACGTR 2 cut(s) 837, 863
Psp6I CCWGG 2 cut(s) 280, 854
PspCI CACGTG 2 cut(s) 837, 863
PspGI CCWGG 2 cut(s) 280, 854
PspN4I GGNNCC 5 cut(s) 290, 291, 292, 320, 852
PspOMI GGGCCC 1 cut(s) 289
PspPI GGNCC 3 cut(s) 221, 289, 290
PsuI RGATCY 1 cut(s) 714
PsyI GACNNNGTC 1 cut(s) 802
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 2 cut(s) 308, 748
Sau3AI GATC 2 cut(s) 175, 714
Sau96I GGNCC 3 cut(s) 221, 289, 290
ScrFI CCNGG 4 cut(s) 282, 478, 492, 856
SduI GDGCHC 4 cut(s) 293, 348, 855, 862
SetI ASST 9 cut(s) 185, 208, 216, 443, 617, 749, 839, 865, 925
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 4 cut(s) 46, 78, 399, 844
SsiI CCGC 2 cut(s) 305, 308
SspMI CTAG 2 cut(s) 203, 818
StyD4I CCNGG 4 cut(s) 280, 476, 490, 854
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 3 cut(s) 160, 278, 380
TaiI ACGT 2 cut(s) 839, 865
TaqI TCGA 1 cut(s) 515
TasI AATT 4 cut(s) 46, 78, 399, 844
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 3 cut(s) 41, 246, 329
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 1 cut(s) 798
TseI GCWGC 1 cut(s) 747
TspDTI ATGAA 4 cut(s) 33, 127, 134, 223
TspRI CASTG 1 cut(s) 798
Tth111I GACNNNGTC 1 cut(s) 802
Van91I CCANNNNNTGG 1 cut(s) 341
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 2 cut(s) 203, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.