RLG00000024734

Steroid 5-alpha-reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014100
Physical Location & Seq
Reverse (-)
38623688 .. 38624470
783 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024734

Sequence Viewer

Length: 783 bp
ATGGCCTCATCGGATCAGACCTTCTTCAACAACTCCCTCCTAGCTCTATGCCTCATAGGCCCTCCCACCTTCATCTCCCTCAGATTCCTCCAAGCCCCATATGGCAAGCACAACCGTCCAGGGTGGGGCCCCACCATGTCTCCGCCGCTGGCTTGGTTCCTCATGGAATCACCAACCCTTTGGCTCACTCTACTCCTCTTCCCCCATGGCCAACACAGTTCCAATCCCCAAGCCTTAATTCTAATGACCCCCTTTCTTTTCCACTATTTCAACCGAACCTGCCTCTACCCACTTCGCCTCTACCGGAATGCCACCCGGAAAACCACCCCCGGTTTTCCGGTCAGCGTGGCGTCGATGGCATTTGTGTTCAATCTGTTGAATGGTTATTTGCAATCCAGATGGGTGTCTCACTACAAGGACTATGAGAGTGATCATGGGTGGTTTTGGTGTAGGTTTTTTGTTGGGTTGGTTGTGTTTTTGTGTGGGATGGGTGTGAATGTGTGGTCTGATAGGGTTTTAGTGGGTTTGAAAAAAGAGGGTGGAGGGTATAAGATCCCCAAAGGAGGGTGGTTTGAGTTGGTGAGCTGCCCTAACTACTTTGGGGAAATAGTGGAGTGGTTAGGATGGGCAGTGATGACTTGGTCATGGGCAGGACTCGGGTTTATGCTATACACGTGTGCCAATTTGGTGCCCAGGGCACGTGCGAACCATAGGTGGTATTTGGAGAAGTTTGGGGAGGACTATCCCAAGTCCAGAAAAGCTGTTATCCCTTTCTTGTATTGA

Protein Analysis

261

Amino Acids

30.0

Weight (kDa)

9.41

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 112 - 260 1.6e-56 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014100)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38050
fragaria_vesca FvH4_6g12130
malus_domestica MD04G1146500.v1.1 MD12G1159700.v1.1
prunus_persica Prupe.6G270300_v2.0.a1 Prupe.6G270300_v2.0.a1
pyrus_communis pycom12g15140
rosa_chinensis RchiOBHm_Chr3g0463931
rosa_laevigata RLG00000024734
rosa_multiflora Rmu_co8513843.1_g000001 Rmu_sc0006492.1_g000004
rosa_roxburghii Rroxscaffold_6G00416130
rosa_rugosa Rorug03G0069400
rosa_samantha Rh3BG130400 Rh3CG132800 Rh3DG131600
rosa_wichuraiana Rw3G010660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 688
AciI CCGC 2 cut(s) 143, 146
AclWI GGATC 2 cut(s) 21, 547
AcoI YGGCCR 1 cut(s) 208
AcvI CACGTG 2 cut(s) 675, 701
AcyI GRCGYC 1 cut(s) 350
AfiI CCNNNNNNNGG 3 cut(s) 125, 563, 564
AflIII ACRYGT 2 cut(s) 672, 674
AgsI TTSAA 5 cut(s) 28, 271, 370, 379, 529
AjnI CCWGG 2 cut(s) 118, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 3 cut(s) 44, 585, 761
AluI AGCT 3 cut(s) 44, 585, 761
Alw26I GTCTC 2 cut(s) 144, 411
AlwI GGATC 2 cut(s) 21, 547
Ama87I CYCGRG 1 cut(s) 656
AoxI GGCC 4 cut(s) 3, 58, 127, 208
ApaI GGGCCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 585
AspS9I GGNCC 3 cut(s) 59, 127, 128
AsuC2I CCSGG 2 cut(s) 316, 330
AsuHPI GGTGA 2 cut(s) 162, 592
AvaI CYCGRG 1 cut(s) 656
BaeGI GKGCMC 3 cut(s) 131, 693, 700
BalI TGGCCA 1 cut(s) 210
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 131
BbrPI CACGTG 2 cut(s) 675, 701
BbvI GCAGC 1 cut(s) 572
BccI CCATC 4 cut(s) 349, 393, 481, 618
BciT130I CCWGG 2 cut(s) 120, 694
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 2 cut(s) 316, 330
BcoDI GTCTC 2 cut(s) 144, 411
BfaI CTAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 287
BglI GCCNNNNNGGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 146, 586
BlsI GCNGC 2 cut(s) 147, 587
Bme1390I CCNGG 4 cut(s) 120, 316, 330, 694
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 59, 127, 128
BmiI GGNNCC 5 cut(s) 128, 129, 130, 158, 690
BmrFI CCNGG 4 cut(s) 120, 316, 330, 694
BpuMI CCSGG 2 cut(s) 316, 330
BsaAI YACGTR 2 cut(s) 675, 701
BsaHI GRCGYC 1 cut(s) 350
BsaJI CCNNGG 5 cut(s) 119, 205, 328, 692, 693
BsaWI WCCGGW 2 cut(s) 303, 337
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 563, 564
BseBI CCWGG 2 cut(s) 120, 694
BseDI CCNNGG 5 cut(s) 119, 205, 328, 692, 693
BseGI GGATG 2 cut(s) 492, 629
BseLI CCNNNNNNNGG 3 cut(s) 125, 563, 564
BseMII CTCAG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 185
BseSI GKGCMC 3 cut(s) 131, 693, 700
BseXI GCAGC 1 cut(s) 572
BshFI GGCC 4 cut(s) 5, 60, 129, 210
BshNI GGYRCC 1 cut(s) 688
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 4 cut(s) 304, 316, 330, 338
BslI CCNNNNNNNGG 3 cut(s) 125, 563, 564
BsmAI GTCTC 2 cut(s) 144, 411
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 4 cut(s) 5, 60, 129, 210
BsoBI CYCGRG 1 cut(s) 656
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 3 cut(s) 131, 693, 700
Bsp143I GATC 3 cut(s) 13, 430, 552
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 2 cut(s) 143, 146
BspANI GGCC 4 cut(s) 5, 60, 129, 210
BspCNI CTCAG 1 cut(s) 93
BspLI GGNNCC 5 cut(s) 128, 129, 130, 158, 690
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 2 cut(s) 21, 547
BspT107I GGYRCC 1 cut(s) 688
BssECI CCNNGG 5 cut(s) 119, 205, 328, 692, 693
BssMI GATC 3 cut(s) 13, 430, 552
BssNI GRCGYC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 120, 694
Bst4CI ACNGT 2 cut(s) 116, 218
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 350
BstBAI YACGTR 2 cut(s) 675, 701
BstC8I GCNNGC 2 cut(s) 107, 150
BstDEI CTNAG 1 cut(s) 80
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 492, 629
BstKTI GATC 3 cut(s) 16, 433, 555
BstMAI GTCTC 2 cut(s) 144, 411
BstMBI GATC 3 cut(s) 13, 430, 552
BstMWI GCNNNNNNNGC 2 cut(s) 57, 356
BstNI CCWGG 2 cut(s) 120, 694
BstSCI CCNGG 4 cut(s) 118, 314, 328, 692
BstSLI GKGCMC 3 cut(s) 131, 693, 700
BstV1I GCAGC 1 cut(s) 572
BstX2I RGATCY 1 cut(s) 552
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 4 cut(s) 5, 60, 129, 210
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 492, 629
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 2 cut(s) 107, 150
Cfr13I GGNCC 3 cut(s) 59, 127, 128
CseI GACGC 1 cut(s) 339
CviAII CATG 5 cut(s) 136, 163, 206, 434, 645
DdeI CTNAG 1 cut(s) 80
DpnI GATC 3 cut(s) 15, 432, 554
DpnII GATC 3 cut(s) 13, 430, 552
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 131
Eco72I CACGTG 2 cut(s) 675, 701
Eco88I CYCGRG 1 cut(s) 656
EcoO109I RGGNCCY 3 cut(s) 59, 127, 128
EcoRII CCWGG 2 cut(s) 118, 692
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 5 cut(s) 139, 166, 209, 437, 648
FatI CATG 5 cut(s) 135, 162, 205, 433, 644
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 2 cut(s) 146, 586
FokI GGATG 2 cut(s) 499, 636
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 146, 586
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 146, 586
HaeIII GGCC 4 cut(s) 5, 60, 129, 210
HapII CCGG 4 cut(s) 304, 316, 330, 338
HgaI GACGC 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 5 cut(s) 139, 166, 209, 437, 648
HinfI GANTC 3 cut(s) 84, 167, 654
HpaII CCGG 4 cut(s) 304, 316, 330, 338
HphI GGTGA 2 cut(s) 162, 592
Hpy188I TCNGA 4 cut(s) 13, 18, 83, 508
Hpy188III TCNNGA 2 cut(s) 396, 753
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 31, 79
HpyCH4III ACNGT 2 cut(s) 116, 218
HpyCH4IV ACGT 2 cut(s) 674, 700
HpyCH4V TGCA 1 cut(s) 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 356
HpyF3I CTNAG 1 cut(s) 80
HpySE526I ACGT 2 cut(s) 674, 700
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 5 cut(s) 139, 166, 209, 437, 648
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 3 cut(s) 13, 430, 552
Lsp1109I GCAGC 1 cut(s) 572
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 674, 700
MalI GATC 3 cut(s) 15, 432, 554
MboI GATC 3 cut(s) 13, 430, 552
MboII GAAGA 2 cut(s) 16, 190
MflI RGATCY 1 cut(s) 552
MhlI GDGCHC 3 cut(s) 131, 693, 700
MlsI TGGCCA 1 cut(s) 210
MluCI AATT 2 cut(s) 237, 682
MluNI TGGCCA 1 cut(s) 210
MlyI GAGTC 1 cut(s) 648
Mox20I TGGCCA 1 cut(s) 210
MscI TGGCCA 1 cut(s) 210
MseI TTAA 1 cut(s) 236
Msp20I TGGCCA 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 4 cut(s) 304, 316, 330, 338
MspR9I CCNGG 4 cut(s) 120, 316, 330, 694
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 2 cut(s) 120, 694
MwoI GCNNNNNNNGC 2 cut(s) 57, 356
NciI CCSGG 2 cut(s) 316, 330
NcoI CCATGG 1 cut(s) 205
NdeI CATATG 1 cut(s) 100
NdeII GATC 3 cut(s) 13, 430, 552
NlaIII CATG 5 cut(s) 139, 166, 209, 437, 648
NlaIV GGNNCC 5 cut(s) 128, 129, 130, 158, 690
PasI CCCWGGG 1 cut(s) 693
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 2 cut(s) 84, 167
PflFI GACNNNGTC 1 cut(s) 640
PkrI GCNGC 2 cut(s) 147, 587
PleI GAGTC 1 cut(s) 648
PmaCI CACGTG 2 cut(s) 675, 701
PmlI CACGTG 2 cut(s) 675, 701
PpsI GAGTC 1 cut(s) 648
Ppu21I YACGTR 2 cut(s) 675, 701
Psp6I CCWGG 2 cut(s) 118, 692
PspCI CACGTG 2 cut(s) 675, 701
PspGI CCWGG 2 cut(s) 118, 692
PspN4I GGNNCC 5 cut(s) 128, 129, 130, 158, 690
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 3 cut(s) 59, 127, 128
PsuI RGATCY 1 cut(s) 552
PsyI GACNNNGTC 1 cut(s) 640
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 2 cut(s) 146, 586
Sau3AI GATC 3 cut(s) 13, 430, 552
Sau96I GGNCC 3 cut(s) 59, 127, 128
SchI GAGTC 1 cut(s) 648
ScrFI CCNGG 4 cut(s) 120, 316, 330, 694
SduI GDGCHC 3 cut(s) 131, 693, 700
Sse9I AATT 2 cut(s) 237, 682
SsiI CCGC 2 cut(s) 143, 146
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 4 cut(s) 118, 314, 328, 692
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 116, 218
TaiI ACGT 2 cut(s) 677, 703
TaqI TCGA 1 cut(s) 353
TasI AATT 2 cut(s) 237, 682
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 84, 167
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 1 cut(s) 636
TseI GCWGC 1 cut(s) 585
TspDTI ATGAA 1 cut(s) 61
TspRI CASTG 1 cut(s) 636
Tth111I GACNNNGTC 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 98
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.