Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014100

Physical Location & Seq

Genomic Coordinates Reverse (-)

23955053 .. 23955289

Gene Structure 237 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1159700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 237 bp

ATGGCATTTGGGTTCAATATGTTGAATGCGTATCTTCAGGCCAGGTGGGTTCCTCAGTACAAAGATTATGAATCCGACGGGTGGTTTTGGTGGCGGTTTGTGGTTGGGTTGGCGGTGTTTGGTTATGGCATGGGTGTAAATATTTGTTCTGATAGGGTCCTTGTGGGGTTGAAAAAAGAGGGTGGGGGAATAAAGTGCCTGAGCTGCCCAAACTACCATGGGGAGATAATGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003865 GO:0006629 GO:0006694 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009917 GO:0010268 GO:0010817 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016229 GO:0016491 GO:0016627 GO:0016628 GO:0033765 GO:0042445 GO:0042446 GO:0042592 GO:0044238 GO:0048878 GO:0050213 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00905 ko01100 ko01110 map00905 map01100 map01110

Pfam Domains

Protein Families

Steroid_dh

Protein Analysis

79

Amino Acids

8.92

Weight (kDa)

6.54

Isoelectric Point (pI)

40.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Steroid_dh	PF02544	1 - 78	1.6e-13	3-oxo-5-alpha-steroid 4-dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38050
fragaria_vesca	FvH4_6g12130
malus_domestica	MD04G1146500.v1.1 MD12G1159700.v1.1
prunus_persica	Prupe.6G270300_v2.0.a1 Prupe.6G270300_v2.0.a1
pyrus_communis	pycom12g15140
rosa_chinensis	RchiOBHm_Chr3g0463931
rosa_laevigata	RLG00000024734
rosa_multiflora	Rmu_co8513843.1_g000001 Rmu_sc0006492.1_g000004
rosa_roxburghii	Rroxscaffold_6G00416130
rosa_rugosa	Rorug03G0069400
rosa_samantha	Rh3BG130400 Rh3CG132800 Rh3DG131600
rosa_wichuraiana	Rw3G010660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	94, 113
AcuI	CTGAAG	1 cut(s)	20
AfaI	GTAC	1 cut(s)	59
AfiI	CCNNNNNNNGG	1 cut(s)	81
AgsI	TTSAA	3 cut(s)	16, 25, 172
AjnI	CCWGG	1 cut(s)	41
AluBI	AGCT	1 cut(s)	204
AluI	AGCT	1 cut(s)	204
AoxI	GGCC	1 cut(s)	39
ApeKI	GCWGC	1 cut(s)	204
AspS9I	GGNCC	1 cut(s)	157
AvaII	GGWCC	1 cut(s)	157
BbvI	GCAGC	1 cut(s)	191
BciT130I	CCWGG	1 cut(s)	43
BisI	GCNGC	1 cut(s)	205
BlsI	GCNGC	1 cut(s)	206
Bme1390I	CCNGG	1 cut(s)	43
Bme18I	GGWCC	1 cut(s)	157
BmgT120I	GGNCC	1 cut(s)	157
BmiI	GGNNCC	2 cut(s)	51, 158
BmrFI	CCNGG	1 cut(s)	43
Bpu10I	CCTNAGC	1 cut(s)	200
BsaJI	CCNNGG	1 cut(s)	217
Bsc4I	CCNNNNNNNGG	1 cut(s)	81
BseBI	CCWGG	1 cut(s)	43
BseDI	CCNNGG	1 cut(s)	217
BseLI	CCNNNNNNNGG	1 cut(s)	81
BseMII	CTCAG	2 cut(s)	68, 191
BseXI	GCAGC	1 cut(s)	191
BshFI	GGCC	1 cut(s)	41
BslI	CCNNNNNNNGG	1 cut(s)	81
BsmI	GAATGC	1 cut(s)	31
BsnI	GGCC	1 cut(s)	41
Bsp19I	CCATGG	1 cut(s)	217
BspACI	CCGC	2 cut(s)	94, 113
BspANI	GGCC	1 cut(s)	41
BspCNI	CTCAG	2 cut(s)	67, 192
BspLI	GGNNCC	2 cut(s)	51, 158
BssECI	CCNNGG	1 cut(s)	217
BssT1I	CCWWGG	1 cut(s)	217
Bst2UI	CCWGG	1 cut(s)	43
BstDEI	CTNAG	2 cut(s)	54, 200
BstDSI	CCRYGG	1 cut(s)	217
BstMWI	GCNNNNNNNGC	1 cut(s)	204
BstNI	CCWGG	1 cut(s)	43
BstSCI	CCNGG	1 cut(s)	41
BstV1I	GCAGC	1 cut(s)	191
BsuRI	GGCC	1 cut(s)	41
BtgI	CCRYGG	1 cut(s)	217
Cfr13I	GGNCC	1 cut(s)	157
Csp6I	GTAC	1 cut(s)	58
CviAII	CATG	2 cut(s)	130, 218
CviJI	RGCY	2 cut(s)	41, 204
CviKI_1	RGCY	2 cut(s)	41, 204
CviQI	GTAC	1 cut(s)	58
DdeI	CTNAG	2 cut(s)	54, 200
Eco130I	CCWWGG	1 cut(s)	217
Eco47I	GGWCC	1 cut(s)	157
Eco57I	CTGAAG	1 cut(s)	20
EcoO109I	RGGNCCY	1 cut(s)	157
EcoRII	CCWGG	1 cut(s)	41
EcoT14I	CCWWGG	1 cut(s)	217
ErhI	CCWWGG	1 cut(s)	217
FaeI	CATG	2 cut(s)	133, 221
FaiI	YATR	5 cut(s)	20, 69, 126, 131, 219
FatI	CATG	2 cut(s)	129, 217
Fnu4HI	GCNGC	1 cut(s)	205
Fsp4HI	GCNGC	1 cut(s)	205
GluI	GCNGC	1 cut(s)	205
HaeIII	GGCC	1 cut(s)	41
Hin1II	CATG	2 cut(s)	133, 221
HinfI	GANTC	1 cut(s)	71
Hpy188I	TCNGA	2 cut(s)	76, 151
Hpy99I	CGWCG	1 cut(s)	80
HpyF10VI	GCNNNNNNNGC	1 cut(s)	204
HpyF3I	CTNAG	2 cut(s)	54, 200
Hsp92II	CATG	2 cut(s)	133, 221
LpnPI	CCDG	4 cut(s)	23, 28, 55, 212
Lsp1109I	GCAGC	1 cut(s)	191
MboII	GAAGA	1 cut(s)	26
MmeI	TCCRAC	1 cut(s)	99
MnlI	CCTC	2 cut(s)	63, 172
MspR9I	CCNGG	1 cut(s)	43
Mva1269I	GAATGC	1 cut(s)	31
MvaI	CCWGG	1 cut(s)	43
MwoI	GCNNNNNNNGC	1 cut(s)	204
NcoI	CCATGG	1 cut(s)	217
NlaIII	CATG	2 cut(s)	133, 221
NlaIV	GGNNCC	2 cut(s)	51, 158
PctI	GAATGC	1 cut(s)	31
PfeI	GAWTC	1 cut(s)	71
PkrI	GCNGC	1 cut(s)	206
PpuMI	RGGWCCY	1 cut(s)	157
Psp5II	RGGWCCY	1 cut(s)	157
Psp6I	CCWGG	1 cut(s)	41
PspGI	CCWGG	1 cut(s)	41
PspN4I	GGNNCC	2 cut(s)	51, 158
PspPI	GGNCC	1 cut(s)	157
PspPPI	RGGWCCY	1 cut(s)	157
RsaI	GTAC	1 cut(s)	59
RsaNI	GTAC	1 cut(s)	58
SatI	GCNGC	1 cut(s)	205
Sau96I	GGNCC	1 cut(s)	157
ScrFI	CCNGG	1 cut(s)	43
SetI	ASST	2 cut(s)	47, 206
SgeI	CNNG	8 cut(s)	50, 54, 55, 91, 142, 173, 211, 230
SinI	GGWCC	1 cut(s)	157
SsiI	CCGC	2 cut(s)	94, 113
SspI	AATATT	1 cut(s)	142
StyD4I	CCNGG	1 cut(s)	41
StyI	CCWWGG	1 cut(s)	217
TatI	WGTACW	1 cut(s)	57
TfiI	GAWTC	1 cut(s)	71
TseI	GCWGC	1 cut(s)	204
TspDTI	ATGAA	1 cut(s)	84
VpaK11BI	GGWCC	1 cut(s)	157

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.