FvH4_6g14100

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006939
Physical Location & Seq
Forward (+)
8630302 .. 8634945
4644 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14100.t4

Sequence Viewer

Length: 1743 bp
ATGGGCTCCATCAATGAAGCCGAGAATGCCACTGAACTTGAAGCTCTGTGCAAGCAAGATTCTTCATGGTACCCCTGCCATGTCTCTCTCAGCTCTACTGAAGACAGCCTAATTGTCGACTTTGGGAGGCAGGAGTTAGAAGATATGGTGTTGAACAAGGATGAAGCACTTATGCGTCTTCGTTTTCGTTCTGGTCCTCTTCAGGGTGACGACTGCTCTCACATTGAAGGAGAGCATGTTCTTGCGATACATAAATCACCATTTAAGAGCTACTTATATGATGCTAAGGTGGAAAAGGTAACCAGAGTGAGACACTCTACAAGGGTCTATTGCAGATGCAGTTTTATGATTCTGTGGCTTCACCCAGACTTCAAAGGACAGATGGTCACTATCACATCCAGTTCAATCATGAAACTAGCAAGCAAAAGCATCAATTCCCATCCTACTGTTGCTGCATTATTCAAATCAGTTAAGCAAATGGGTTTATATACTGCACCTCTCCTTCCAATCATGCATGAGGACATTGATGTTGAATTCGACCTCAATAAATTATTGGGAAAGCAAATAGAAGAGATCAACATTTCAGCTAATAGAGTTACAAATGAAATTACAGTAGACATAATAGAGGGGGTTAAAGCTGATAGCAGTGGACATGTTACTGAGTCCAAAATAGGCACTTCAAAAGCTCAGGTTTCTCATGACCAGGATCAATTGAAATCTGTTGCTAACAGATCTGGTAACCTGGAAGTAAACAAAGAAGATGAGGACCCACATCCCCCTTTCTTATCCAAACAGGAAGAGCATTCAGAACACAGGTGTCACATTAGTCCTCTTGCTGCCCGTGCAGCTCTGGCCTCCCTAGTATCACTTACTCACAAGCATATTGCTATAAGTGGGACAGAACTTTTCAAGTCCAGTGACAGTACTGATTTGTCTATTAAGGTATCTTCTGATAGAACCGAATCTCCAAAGAATGGAAATGCAAACTTGGGCTCAGGTGCTAGAACAACTCGCTCAAGAGGTCTTAAAGGTTTTGAAAAACAGAACAGTGATTTGCATGATTCTGCTGAAGCCATCAAGTTAAGGGCCGTAACAAATCGAGGATGGTTGACTCGTTCAGCAGTCAAAGAAGAAAAAGATATATCATCTGTGGCAAGTAAACATGGGTCAGAAGAGAGCGAGTCTGCTCAGAGTACAGAATCATATTCTTCTGATGGAACTGACATTGTTCATGGAAACAAAGTATTGACAAAGAAGAATGGTATATCAAAGAAAGCAGTCTCTTCACCACTTCATTCAGAGAGTAATGGCCATAAGGAAAATCTGACATCTGGTGATCTTGGGGTCATTCAAGATGCATATGTTCAGACAAAGACTTGTGCTAAAGACACCAACTCAAGCGTTTCAACCAACTTGAGGAGGTTGACTCGTTCCAGGGTCTCTTGTCAAGACAATTTGATAGTTCCTGAATGCCATGCAGTAGAAAAGGAAAATCGTGAATCAAAAAAGAAGAAAGCTGGCTCTGCATCATCTCAGAATTACAGTACGTCAGGTGAAGATGGTAATAGACAGCACAACTCTGGGGTTGTGAGAAACAGTCGACAAACTGAAGGAAAAATGTCAGGCAGTGGTGACAATAGTCAAGGCCGGAAGAGGAAGTCTAATTCTTCGAGTAGGCAAGAGCAACAGTTTTCTCCACAGTTAAGGTTTCTCCCTCGGACTCGTTCACAGAACAAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

581

Amino Acids

63.86

Weight (kDa)

8.4

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 11 - 136 3.1e-21 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 69
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 974
AccI GTMKAC 3 cut(s) 117, 615, 1600
AclWI GGATC 1 cut(s) 714
AcoI YGGCCR 1 cut(s) 1309
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 4 cut(s) 120, 185, 1089, 1629
AfaI GTAC 4 cut(s) 71, 925, 1195, 1546
AfiI CCNNNNNNNGG 3 cut(s) 203, 974, 1416
AflIII ACRYGT 1 cut(s) 652
AhdI GACNNNNNGTC 1 cut(s) 383
AjnI CCWGG 3 cut(s) 702, 741, 1433
AloI GAACNNNNNNTCC 4 cut(s) 222, 254, 949, 981
AluBI AGCT 8 cut(s) 44, 93, 270, 587, 638, 686, 848, 1517
AluI AGCT 8 cut(s) 44, 93, 270, 587, 638, 686, 848, 1517
Alw26I GTCTC 4 cut(s) 88, 304, 1285, 1444
AlwI GGATC 1 cut(s) 714
AoxI GGCC 4 cut(s) 852, 1086, 1309, 1645
ApeKI GCWGC 3 cut(s) 452, 836, 845
ApoI RAATTY 1 cut(s) 533
Asp718I GGTACC 1 cut(s) 69
AspS9I GGNCC 3 cut(s) 194, 766, 1086
AsuHPI GGTGA 7 cut(s) 218, 249, 353, 1278, 1346, 1565, 1643
AvaII GGWCC 2 cut(s) 194, 766
BalI TGGCCA 1 cut(s) 1311
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 2 cut(s) 8, 995
BbsI GAAGAC 2 cut(s) 108, 170
BbvI GCAGC 3 cut(s) 439, 823, 857
BccI CCATC 7 cut(s) 17, 376, 447, 1082, 1098, 1208, 1553
BceAI ACGGC 1 cut(s) 1073
BciT130I CCWGG 3 cut(s) 704, 743, 1435
BcoDI GTCTC 4 cut(s) 88, 304, 1285, 1444
BfaI CTAG 3 cut(s) 416, 860, 1002
BglII AGATCT 1 cut(s) 731
BisI GCNGC 3 cut(s) 453, 837, 846
BlsI GCNGC 3 cut(s) 454, 838, 847
BmcAI AGTACT 1 cut(s) 925
Bme1390I CCNGG 3 cut(s) 704, 743, 1435
Bme18I GGWCC 2 cut(s) 194, 766
BmeRI GACNNNNNGTC 1 cut(s) 383
BmgT120I GGNCC 3 cut(s) 194, 766, 1086
BmiI GGNNCC 3 cut(s) 7, 71, 768
BmrFI CCNGG 3 cut(s) 704, 743, 1435
BmsI GCATC 5 cut(s) 271, 326, 438, 1345, 1535
BoxI GACNNNNGTC 1 cut(s) 1638
BpiI GAAGAC 2 cut(s) 108, 170
BplI GAGNNNNNCTC 2 cut(s) 1411, 1443
Bpu10I CCTNAGC 3 cut(s) 285, 687, 994
BpuEI CTTGAG 3 cut(s) 1000, 1381, 1435
BsaI GGTCTC 1 cut(s) 1444
BsaJI CCNNGG 2 cut(s) 1434, 1715
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 949, 979
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 974, 1416
Bse1I ACTGG 2 cut(s) 399, 915
BseBI CCWGG 3 cut(s) 704, 743, 1435
BseDI CCNNGG 2 cut(s) 1434, 1715
BseGI GGATG 5 cut(s) 166, 395, 439, 772, 1109
BseLI CCNNNNNNNGG 3 cut(s) 203, 974, 1416
BseMII CTCAG 6 cut(s) 103, 651, 701, 1008, 1202, 1547
BseNI ACTGG 2 cut(s) 399, 915
BseRI GAGGAG 1 cut(s) 1432
BseXI GCAGC 3 cut(s) 439, 823, 857
BsgI GTGCAG 2 cut(s) 477, 864
BshFI GGCC 4 cut(s) 854, 1088, 1311, 1647
BshNI GGYRCC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 1648
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 3 cut(s) 203, 974, 1416
BsmAI GTCTC 4 cut(s) 88, 304, 1285, 1444
BsmFI GGGAC 1 cut(s) 910
BsmI GAATGC 3 cut(s) 31, 802, 1475
BsnI GGCC 4 cut(s) 854, 1088, 1311, 1647
Bso31I GGTCTC 1 cut(s) 1444
Bsp1286I GDGCHC 2 cut(s) 8, 995
Bsp143I GATC 4 cut(s) 573, 706, 731, 1336
BspANI GGCC 4 cut(s) 854, 1088, 1311, 1647
BspCNI CTCAG 6 cut(s) 102, 652, 700, 1007, 1201, 1546
BspHI TCATGA 2 cut(s) 408, 697
BspLI GGNNCC 3 cut(s) 7, 71, 768
BspPI GGATC 1 cut(s) 714
BspQI GCTCTTC 1 cut(s) 792
BspT107I GGYRCC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 1444
BsrI ACTGG 2 cut(s) 399, 915
BssECI CCNNGG 2 cut(s) 1434, 1715
BssMI GATC 4 cut(s) 573, 706, 731, 1336
Bst2UI CCWGG 3 cut(s) 704, 743, 1435
Bst4CI ACNGT 8 cut(s) 448, 613, 923, 1049, 1544, 1598, 1689, 1701
Bst6I CTCTTC 6 cut(s) 204, 564, 792, 1167, 1288, 1646
BstC8I GCNNGC 3 cut(s) 53, 421, 1519
BstDEI CTNAG 7 cut(s) 89, 285, 660, 687, 994, 1188, 1533
BstEII GGTNACC 2 cut(s) 298, 737
BstF5I GGATG 5 cut(s) 166, 395, 439, 772, 1109
BstKTI GATC 4 cut(s) 576, 709, 734, 1339
BstMAI GTCTC 4 cut(s) 88, 304, 1285, 1444
BstMBI GATC 4 cut(s) 573, 706, 731, 1336
BstMWI GCNNNNNNNGC 5 cut(s) 26, 842, 845, 851, 1523
BstNI CCWGG 3 cut(s) 704, 743, 1435
BstNSI RCATGY 2 cut(s) 239, 656
BstPAI GACNNNNGTC 1 cut(s) 1638
BstPI GGTNACC 2 cut(s) 298, 737
BstSCI CCNGG 3 cut(s) 702, 741, 1433
BstV1I GCAGC 3 cut(s) 439, 823, 857
BstV2I GAAGAC 2 cut(s) 108, 170
BstX2I RGATCY 1 cut(s) 731
BstYI RGATCY 1 cut(s) 731
BsuRI GGCC 4 cut(s) 854, 1088, 1311, 1647
BtsCI GGATG 5 cut(s) 166, 395, 439, 772, 1109
BtsI GCAGTG 2 cut(s) 652, 1633
BtsIMutI CAGTG 5 cut(s) 30, 652, 922, 1054, 1633
Cac8I GCNNGC 3 cut(s) 53, 421, 1519
CciI TCATGA 2 cut(s) 408, 697
Cfr13I GGNCC 3 cut(s) 194, 766, 1086
CseI GACGC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 70, 924, 1194, 1545
CspCI CAANNNNNGTGG 2 cut(s) 19, 54
CviQI GTAC 4 cut(s) 70, 924, 1194, 1545
DdeI CTNAG 7 cut(s) 89, 285, 660, 687, 994, 1188, 1533
DpnI GATC 4 cut(s) 575, 708, 733, 1338
DpnII GATC 4 cut(s) 573, 706, 731, 1336
DriI GACNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 1 cut(s) 1309
Eam1104I CTCTTC 6 cut(s) 204, 564, 792, 1167, 1288, 1646
Eam1105I GACNNNNNGTC 1 cut(s) 383
EarI CTCTTC 6 cut(s) 204, 564, 792, 1167, 1288, 1646
Eco24I GRGCYC 2 cut(s) 8, 995
Eco31I GGTCTC 1 cut(s) 1444
Eco47I GGWCC 2 cut(s) 194, 766
Eco57I CTGAAG 4 cut(s) 120, 185, 1089, 1629
Eco91I GGTNACC 2 cut(s) 298, 737
EcoO109I RGGNCCY 1 cut(s) 766
EcoO65I GGTNACC 2 cut(s) 298, 737
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 3 cut(s) 702, 741, 1433
EcoT22I ATGCAT 2 cut(s) 516, 1360
EcoT38I GRGCYC 2 cut(s) 8, 995
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 910
FauNDI CATATG 1 cut(s) 1360
FblI GTMKAC 3 cut(s) 117, 615, 1600
Fnu4HI GCNGC 3 cut(s) 453, 837, 846
FokI GGATG 5 cut(s) 173, 382, 426, 759, 1116
FriOI GRGCYC 2 cut(s) 8, 995
Fsp4HI GCNGC 3 cut(s) 453, 837, 846
FspBI CTAG 3 cut(s) 416, 860, 1002
GluI GCNGC 3 cut(s) 453, 837, 846
HaeIII GGCC 4 cut(s) 854, 1088, 1311, 1647
HapII CCGG 1 cut(s) 1648
HgaI GACGC 1 cut(s) 164
HincII GTYRAC 4 cut(s) 118, 1110, 1425, 1601
HindII GTYRAC 4 cut(s) 118, 1110, 1425, 1601
HpaII CCGG 1 cut(s) 1648
HphI GGTGA 7 cut(s) 218, 249, 353, 1278, 1346, 1565, 1643
Hpy166II GTNNAC 9 cut(s) 118, 616, 650, 751, 1110, 1160, 1425, 1601, 1727
Hpy188III TCNNGA 7 cut(s) 409, 698, 1017, 1352, 1448, 1466, 1496
Hpy8I GTNNAC 9 cut(s) 118, 616, 650, 751, 1110, 1160, 1425, 1601, 1727
HpyAV CCTTC 3 cut(s) 221, 512, 1604
HpyCH4III ACNGT 8 cut(s) 448, 613, 923, 1049, 1544, 1598, 1689, 1701
HpyCH4IV ACGT 1 cut(s) 1547
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 842, 845, 851, 1523
HpyF3I CTNAG 7 cut(s) 89, 285, 660, 687, 994, 1188, 1533
HpySE526I ACGT 1 cut(s) 1547
KpnI GGTACC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 573, 706, 731, 1336
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 3 cut(s) 439, 823, 857
LweI GCATC 5 cut(s) 271, 326, 438, 1345, 1535
MaeI CTAG 3 cut(s) 416, 860, 1002
MaeII ACGT 1 cut(s) 1547
MalI GATC 4 cut(s) 575, 708, 733, 1338
MboI GATC 4 cut(s) 573, 706, 731, 1336
MfeI CAATTG 1 cut(s) 710
MflI RGATCY 1 cut(s) 731
MhlI GDGCHC 2 cut(s) 8, 995
MlsI TGGCCA 1 cut(s) 1311
MluCI AATT 9 cut(s) 111, 433, 533, 548, 606, 710, 1453, 1537, 1663
MluNI TGGCCA 1 cut(s) 1311
MlyI GAGTC 5 cut(s) 671, 1105, 1190, 1420, 1714
Mox20I TGGCCA 1 cut(s) 1311
Mph1103I ATGCAT 2 cut(s) 516, 1360
MscI TGGCCA 1 cut(s) 1311
MseI TTAA 7 cut(s) 264, 471, 633, 939, 1026, 1082, 1703
Msp20I TGGCCA 1 cut(s) 1311
MspI CCGG 1 cut(s) 1648
MspR9I CCNGG 3 cut(s) 704, 743, 1435
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 3 cut(s) 31, 802, 1475
MvaI CCWGG 3 cut(s) 704, 743, 1435
MwoI GCNNNNNNNGC 5 cut(s) 26, 842, 845, 851, 1523
NdeI CATATG 1 cut(s) 1360
NdeII GATC 4 cut(s) 573, 706, 731, 1336
NlaIV GGNNCC 3 cut(s) 7, 71, 768
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 5 cut(s) 206, 385, 818, 917, 1631
NsiI ATGCAT 2 cut(s) 516, 1360
NspI RCATGY 2 cut(s) 239, 656
PagI TCATGA 2 cut(s) 408, 697
PciI ACATGT 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 792
PctI GAATGC 3 cut(s) 31, 802, 1475
PfeI GAWTC 6 cut(s) 59, 349, 962, 1061, 1199, 1499
PflMI CCANNNNNTGG 1 cut(s) 974
PkrI GCNGC 3 cut(s) 454, 838, 847
PleI GAGTC 5 cut(s) 670, 1105, 1189, 1420, 1714
PpsI GAGTC 5 cut(s) 670, 1105, 1189, 1420, 1714
PpuMI RGGWCCY 1 cut(s) 766
PscI ACATGT 1 cut(s) 652
PshAI GACNNNNGTC 1 cut(s) 1638
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 3 cut(s) 702, 741, 1433
PspEI GGTNACC 2 cut(s) 298, 737
PspGI CCWGG 3 cut(s) 702, 741, 1433
PspN4I GGNNCC 3 cut(s) 7, 71, 768
PspPI GGNCC 3 cut(s) 194, 766, 1086
PspPPI RGGWCCY 1 cut(s) 766
PsuI RGATCY 1 cut(s) 731
RsaI GTAC 4 cut(s) 71, 925, 1195, 1546
RsaNI GTAC 4 cut(s) 70, 924, 1194, 1545
SalI GTCGAC 2 cut(s) 116, 1599
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 7 cut(s) 264, 471, 633, 939, 1026, 1082, 1703
SatI GCNGC 3 cut(s) 453, 837, 846
Sau3AI GATC 4 cut(s) 573, 706, 731, 1336
Sau96I GGNCC 3 cut(s) 194, 766, 1086
ScaI AGTACT 1 cut(s) 925
SchI GAGTC 5 cut(s) 671, 1105, 1190, 1420, 1714
ScrFI CCNGG 3 cut(s) 704, 743, 1435
SduI GDGCHC 2 cut(s) 8, 995
SfaNI GCATC 5 cut(s) 271, 326, 438, 1345, 1535
SinI GGWCC 2 cut(s) 194, 766
SmlI CTYRAG 3 cut(s) 1015, 1396, 1414
SmoI CTYRAG 3 cut(s) 1015, 1396, 1414
Sse9I AATT 9 cut(s) 111, 433, 533, 548, 606, 710, 1453, 1537, 1663
SspMI CTAG 3 cut(s) 416, 860, 1002
StyD4I CCNGG 3 cut(s) 702, 741, 1433
TaaI ACNGT 8 cut(s) 448, 613, 923, 1049, 1544, 1598, 1689, 1701
TaiI ACGT 1 cut(s) 1550
TaqI TCGA 5 cut(s) 117, 537, 1099, 1600, 1670
TasI AATT 9 cut(s) 111, 433, 533, 548, 606, 710, 1453, 1537, 1663
TatI WGTACW 2 cut(s) 923, 1193
TfiI GAWTC 6 cut(s) 59, 349, 962, 1061, 1199, 1499
Tru1I TTAA 7 cut(s) 264, 471, 633, 939, 1026, 1082, 1703
Tru9I TTAA 7 cut(s) 264, 471, 633, 939, 1026, 1082, 1703
TscAI CASTG 5 cut(s) 37, 652, 922, 1054, 1633
TseFI GTSAC 5 cut(s) 206, 385, 818, 917, 1631
TseI GCWGC 3 cut(s) 452, 836, 845
Tsp45I GTSAC 5 cut(s) 206, 385, 818, 917, 1631
TspDTI ATGAA 7 cut(s) 30, 54, 177, 425, 618, 1220, 1283
TspRI CASTG 5 cut(s) 37, 652, 922, 1054, 1633
Van91I CCANNNNNTGG 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 194, 766
XapI RAATTY 1 cut(s) 533
XceI RCATGY 2 cut(s) 239, 656
XmiI GTMKAC 3 cut(s) 117, 615, 1600
XspI CTAG 3 cut(s) 416, 860, 1002
ZrmI AGTACT 1 cut(s) 925
Zsp2I ATGCAT 2 cut(s) 516, 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.