Rorug03G0084500

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006939
Physical Location & Seq
Forward (+)
6540590 .. 6541630
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0084500.1

Sequence Viewer

Length: 441 bp
ATGAAAACACCAGCGGTCTGTCTTATTTTCTTGCTCTCTGTTATTCTGTTCAAGGGTTCCCATGCAGAGCCATACGAATACCTACAGTTTGTCTTGCAATACGCTAGAGGTTCCTGTGTTAACGTCAAGAAATGCATTCCACCGGCGAGCTTGCCGGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCAAGCCGGAGATCGAGTGTAAAGCTGCACAGAAACTACACCATTTCAACAAAAAGCTGATAACTCCGTCGTTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCATGACAGATAAAGATGATATGTCGTTTTGGGAACACGAATATAATAAGCATGGGGTACTGCACCAGCTATCACTCAGACAGCGTATTTTAAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

146

Amino Acids

16.72

Weight (kDa)

9.37

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 120 2.9e-18 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 161, 177
AfaI GTAC 1 cut(s) 360
AgsI TTSAA 3 cut(s) 52, 244, 271
AluBI AGCT 5 cut(s) 150, 221, 253, 370, 400
AluI AGCT 5 cut(s) 150, 221, 253, 370, 400
Alw21I GWGCWC 1 cut(s) 402
AoxI GGCC 3 cut(s) 156, 182, 293
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 2 cut(s) 161, 177
AspS9I GGNCC 2 cut(s) 183, 294
BanII GRGCYC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 208
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 183, 294
BmiI GGNNCC 2 cut(s) 58, 112
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bse118I RCCGGY 2 cut(s) 142, 154
BseMII CTCAG 1 cut(s) 391
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 2 cut(s) 207, 347
BshFI GGCC 3 cut(s) 158, 184, 295
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 3 cut(s) 143, 155, 203
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 3 cut(s) 158, 184, 295
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 3 cut(s) 207, 277, 423
BspACI CCGC 1 cut(s) 14
BspANI GGCC 3 cut(s) 158, 184, 295
BspCNI CTCAG 1 cut(s) 390
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 2 cut(s) 58, 112
BsrFI RCCGGY 2 cut(s) 142, 154
BssAI RCCGGY 2 cut(s) 142, 154
BssMI GATC 3 cut(s) 207, 277, 423
Bst4CI ACNGT 2 cut(s) 87, 169
BstC8I GCNNGC 3 cut(s) 148, 152, 156
BstDEI CTNAG 1 cut(s) 377
BstKTI GATC 3 cut(s) 210, 280, 426
BstMBI GATC 3 cut(s) 207, 277, 423
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 208
BsuRI GGCC 3 cut(s) 158, 184, 295
Cac8I GCNNGC 3 cut(s) 148, 152, 156
CciI TCATGA 1 cut(s) 303
Cfr10I RCCGGY 2 cut(s) 142, 154
Cfr13I GGNCC 2 cut(s) 183, 294
CseI GACGC 1 cut(s) 289
Csp6I GTAC 1 cut(s) 359
CviAII CATG 4 cut(s) 62, 173, 304, 353
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 1 cut(s) 377
DpnI GATC 3 cut(s) 209, 279, 425
DpnII GATC 3 cut(s) 207, 277, 423
EaeI YGGCCR 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 402
FaeI CATG 4 cut(s) 65, 176, 307, 356
FaiI YATR 8 cut(s) 63, 73, 174, 305, 323, 345, 354, 422
FatI CATG 4 cut(s) 61, 172, 303, 352
Fnu4HI GCNGC 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 1 cut(s) 105
GluI GCNGC 1 cut(s) 222
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 3 cut(s) 158, 184, 295
HapII CCGG 3 cut(s) 143, 155, 203
HgaI GACGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 65, 176, 307, 356
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 3 cut(s) 143, 155, 203
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 1 cut(s) 380
Hpy188III TCNNGA 3 cut(s) 127, 304, 411
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 408
HpyCH4III ACNGT 2 cut(s) 87, 169
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 65, 97, 135, 224, 364
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 4 cut(s) 65, 176, 307, 356
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 3 cut(s) 207, 277, 423
LpnPI CCDG 8 cut(s) 24, 127, 156, 168, 216, 310, 380, 396
Lsp1109I GCAGC 1 cut(s) 208
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 123
MalI GATC 3 cut(s) 209, 279, 425
MboI GATC 3 cut(s) 207, 277, 423
MboII GAAGA 1 cut(s) 272
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 2 cut(s) 161, 177
MnlI CCTC 1 cut(s) 101
Mph1103I ATGCAT 1 cut(s) 137
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 2 cut(s) 120, 392
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 3 cut(s) 143, 155, 203
Mva1269I GAATGC 1 cut(s) 135
NaeI GCCGGC 1 cut(s) 156
NdeII GATC 3 cut(s) 207, 277, 423
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 4 cut(s) 65, 176, 307, 356
NlaIV GGNNCC 2 cut(s) 58, 112
NsiI ATGCAT 1 cut(s) 137
PagI TCATGA 1 cut(s) 303
PctI GAATGC 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 156
PkrI GCNGC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 58, 112
PspPI GGNCC 2 cut(s) 183, 294
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 2 cut(s) 120, 392
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 3 cut(s) 207, 277, 423
Sau96I GGNCC 2 cut(s) 183, 294
SduI GDGCHC 1 cut(s) 402
SetI ASST 8 cut(s) 84, 112, 126, 152, 223, 255, 372, 402
SfcI CTRYAG 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 142
Sse9I AATT 2 cut(s) 161, 177
SsiI CCGC 1 cut(s) 14
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 402
TaaI ACNGT 2 cut(s) 87, 169
TaiI ACGT 1 cut(s) 126
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 161, 177
Tru1I TTAA 2 cut(s) 120, 392
Tru9I TTAA 2 cut(s) 120, 392
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 17, 161
TspGWI ACGGA 1 cut(s) 252
XapI RAATTY 2 cut(s) 161, 177
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.