Rroxscaffold_6G00414020

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006939
Physical Location & Seq
Reverse (-)
36351874 .. 36356952
5079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00414020.1

Sequence Viewer

Length: 1872 bp
ATGAAAATGGACTCTATCAGAGAAGCGGAGAGTGCCACTGAACTTGAAGCCTTGTGCAAGCAGGATTCTTCTTGGTATCCCTGCCAGGTCTCCCTCAGGCACTTGCCGTCACCAATTGCTTTTAAATTTCAAGTTTTGGGCATGCATTACAAATGTATTGAGAGGTGGCTCCTGATTATGGTCCACAGCTCTACTGAAGATAGCCTAATTGTCGACTTTGGGAGGCAGGAGTTAGAAGATATGGTGTTGAACAAGGAAGAAGCACTTATGCGTCTTCGTTTTCGTTCTGCTCCTCTTCAGGGTGATGACTGCTCTCGCATTGAAGGAGAGCATGTTCTTTCCATCCATAAATCACCATTTAAGAGCCACTTATATGATGCCAAGGTGCAAAAGGTGACCAGAGTGAGACACTCTACAAGGGTCTATTGCAGATGCAGTTTTATGATTAAGTGGCTTCACCCAGCCTTCAAAGGACAGATGGTCACTGTCCCCTCTAGTTCAATCATGAAACTAGCAAGCAAAAGCATCAATTCTCATCCAACTGTTGCTGCATTATTGAAATCAGTTAAGCAAATGGGTTTATATACTGCACCTCCCCTTCCCATCATGCATGAAGACAATGATGTTGAATTCGACCTCAATAAATTATTAGGAAAGCAAATAGAAGAGATCAACATTTCAGCTAATGGAGTCACAAATGAAACTACAGTAGACATATTAGAGGGGTTGAAGGTAACTAGCCCTACTCAATACTTCTTGGTTGTGCATGCAGCTGTTGACAATGGACATGTTACTGCATCCAAAGTAGGCACTTCAAAAGCTCAGGTTTCTCATGACCAGGATCAATTGAAATCTGTTACTAACAGACCTGGTAACCTGGAAGTAAACATAGAAGATGAGGACCCACAAGCCCCTCTCTCATCCAAACAAGAAGAGCATTCAGAACACAGGTCTCATATTAGTCCTCTTGCTGCCCGTGCAGCTCTGGCCTCCCTAGTGTCACTTACTCACAAGCATATTGCTATAAGTGGGACTGAACTTTTCAAGTGCAGTGACAGTAATAATATGTCTATTATGGTATCTTCTACTACAATCAAATCTCCACAAAATGGAAATGCGAACTCAGGTGCTAGAACAACTCGCTCAACAGTTCAGAAAGGTTTGGGAATTCAGAACAGTGATTTGCATGATTCTACTGAAGCCATCGAGTTAAGGGTCTTAACCAACACAAGAAGGTTGACACGTCTAGCAGTCAAAGAAGAAAAAGATATATCATCTATGGAAATTAAACAAGGGTCGGAAGAAAGTGAGTCTGCTCAGATTACAGGATCATATTCTTCTGAAGGAACTGACATTGTTCATGAAAGCAAAGTACTGAGAAAGAAGAATGATATATCAAACAAAGCAGTCTCTTCACCACTTCATTCAGAGAGTAACGGCCCTAAGGAAAATCTGACGTCTGGTGATCTTGAGGCGATTCAAGATGCATATGTTCGGATGAAGAGTTGTGCTAAAGACACCAGGTCAAGTGTTTCAACCAACATGAGGAGGTTGACTCGTTCCATGGTCTCTTGTAAAGACAATTTGATAGTTCCTGAAAGCCATGCAGTGGAAAAGGAAAATCGTGAATCAAAAAAGAAGAAAGCTGTCTCTGCGACATCTCATAATGACAGTACACAAGGTGAAGAAAGTAATAGAAAGCACATGTCCAGTGCTGCCAGAAACAGTCGACAAACTGAAGGAAAAATGTCAGGCAGTGGTGACAATAGTCATGGCCAGAAGAGGAAGTCTACTTCTTTGAGCAGGCAAGAGCAACGGTTTTCTCCACGATTAAGGTTTCTCCCTCGCACTCGTTCACAAAACAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

623

Amino Acids

69.24

Weight (kDa)

8.97

Isoelectric Point (pI)

58.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 13 - 169 4.1e-19 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1460
AccB7I CCANNNNNTGG 2 cut(s) 1109, 1609
AccI GTMKAC 4 cut(s) 213, 711, 1729, 1790
AciI CCGC 1 cut(s) 26
AclWI GGATC 2 cut(s) 849, 1336
AcoI YGGCCR 1 cut(s) 1774
AcsI RAATTY 3 cut(s) 125, 629, 1167
AcuI CTGAAG 5 cut(s) 216, 281, 1218, 1362, 1758
AcyI GRCGYC 1 cut(s) 1457
AdeI CACNNNGTG 1 cut(s) 1682
AfaI GTAC 2 cut(s) 1374, 1675
AfiI CCNNNNNNNGG 5 cut(s) 178, 299, 1109, 1545, 1609
AflIII ACRYGT 3 cut(s) 787, 1241, 1704
AhdI GACNNNNNGTC 2 cut(s) 479, 1522
AjiI CACGTC 1 cut(s) 1244
AjnI CCWGG 5 cut(s) 84, 837, 868, 876, 1520
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 6 cut(s) 189, 683, 773, 821, 983, 1646
AluI AGCT 6 cut(s) 189, 683, 773, 821, 983, 1646
Alw26I GTCTC 6 cut(s) 94, 400, 957, 1414, 1573, 1654
AlwI GGATC 2 cut(s) 849, 1336
AoxI GGCC 3 cut(s) 987, 1438, 1774
ApeKI GCWGC 5 cut(s) 548, 770, 971, 980, 1715
ApoI RAATTY 3 cut(s) 125, 629, 1167
AspS9I GGNCC 3 cut(s) 181, 901, 1439
AsuHPI GGTGA 9 cut(s) 102, 314, 345, 406, 449, 1407, 1475, 1694, 1772
AvaII GGWCC 2 cut(s) 181, 901
AxyI CCTNAGG 2 cut(s) 95, 1443
BalI TGGCCA 1 cut(s) 1776
BbsI GAAGAC 2 cut(s) 266, 621
BbvI GCAGC 5 cut(s) 535, 782, 958, 992, 1702
BccI CCATC 4 cut(s) 350, 472, 611, 1211
BceAI ACGGC 2 cut(s) 91, 1453
BciT130I CCWGG 5 cut(s) 86, 839, 870, 878, 1522
BciVI GTATCC 1 cut(s) 87
BcoDI GTCTC 6 cut(s) 94, 400, 957, 1414, 1573, 1654
BfaI CTAG 6 cut(s) 495, 512, 738, 995, 1131, 1247
BfmI CTRYAG 1 cut(s) 705
BfuI GTATCC 1 cut(s) 87
BisI GCNGC 5 cut(s) 549, 771, 972, 981, 1716
BlsI GCNGC 5 cut(s) 550, 772, 973, 982, 1717
BmcAI AGTACT 1 cut(s) 1374
Bme1390I CCNGG 5 cut(s) 86, 839, 870, 878, 1522
Bme18I GGWCC 2 cut(s) 181, 901
BmeRI GACNNNNNGTC 2 cut(s) 479, 1522
BmgBI CACGTC 1 cut(s) 1244
BmgT120I GGNCC 3 cut(s) 181, 901, 1439
BmiI GGNNCC 2 cut(s) 170, 903
BmrFI CCNGG 5 cut(s) 86, 839, 870, 878, 1522
BmsI GCATC 5 cut(s) 367, 422, 534, 806, 1474
BoxI GACNNNNGTC 1 cut(s) 1767
BpiI GAAGAC 2 cut(s) 266, 621
BplI GAGNNNNNCTC 2 cut(s) 1540, 1572
Bpu10I CCTNAGC 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 1490
BsaHI GRCGYC 1 cut(s) 1457
BsaI GGTCTC 3 cut(s) 94, 957, 1573
BsaJI CCNNGG 2 cut(s) 381, 1563
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 577, 607
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 299, 1109, 1545, 1609
Bse1I ACTGG 1 cut(s) 1710
Bse21I CCTNAGG 2 cut(s) 95, 1443
BseBI CCWGG 5 cut(s) 86, 839, 870, 878, 1522
BseDI CCNNGG 2 cut(s) 381, 1563
BseGI GGATG 5 cut(s) 342, 535, 797, 920, 1503
BseLI CCNNNNNNNGG 5 cut(s) 178, 299, 1109, 1545, 1609
BseMII CTCAG 5 cut(s) 109, 836, 1137, 1331, 1367
BseNI ACTGG 1 cut(s) 1710
BseRI GAGGAG 2 cut(s) 282, 1561
BseXI GCAGC 5 cut(s) 535, 782, 958, 992, 1702
BseYI CCCAGC 1 cut(s) 460
BsgI GTGCAG 3 cut(s) 573, 999, 1069
BshFI GGCC 3 cut(s) 989, 1440, 1776
BslFI GGGAC 2 cut(s) 473, 1045
BslI CCNNNNNNNGG 5 cut(s) 178, 299, 1109, 1545, 1609
BsmAI GTCTC 6 cut(s) 94, 400, 957, 1414, 1573, 1654
BsmFI GGGAC 2 cut(s) 473, 1045
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 3 cut(s) 989, 1440, 1776
Bso31I GGTCTC 3 cut(s) 94, 957, 1573
Bsp143I GATC 4 cut(s) 669, 841, 1328, 1465
Bsp19I CCATGG 1 cut(s) 1563
BspACI CCGC 1 cut(s) 26
BspANI GGCC 3 cut(s) 989, 1440, 1776
BspCNI CTCAG 5 cut(s) 108, 835, 1136, 1330, 1368
BspHI TCATGA 3 cut(s) 504, 832, 1360
BspLI GGNNCC 2 cut(s) 170, 903
BspPI GGATC 2 cut(s) 849, 1336
BspQI GCTCTTC 1 cut(s) 927
BspTNI GGTCTC 3 cut(s) 94, 957, 1573
BsrI ACTGG 1 cut(s) 1710
BssECI CCNNGG 2 cut(s) 381, 1563
BssMI GATC 4 cut(s) 669, 841, 1328, 1465
BssNI GRCGYC 1 cut(s) 1457
BssT1I CCWWGG 2 cut(s) 381, 1563
Bst2UI CCWGG 5 cut(s) 86, 839, 870, 878, 1522
Bst4CI ACNGT 9 cut(s) 487, 544, 709, 1058, 1150, 1178, 1673, 1727, 1818
Bst6I CTCTTC 6 cut(s) 300, 660, 927, 1417, 1496, 1775
BstACI GRCGYC 1 cut(s) 1457
BstC8I GCNNGC 5 cut(s) 59, 143, 517, 768, 1805
BstDEI CTNAG 6 cut(s) 95, 822, 1123, 1317, 1376, 1443
BstDSI CCRYGG 1 cut(s) 1563
BstEII GGTNACC 2 cut(s) 394, 872
BstF5I GGATG 5 cut(s) 342, 535, 797, 920, 1503
BstKTI GATC 4 cut(s) 672, 844, 1331, 1468
BstMAI GTCTC 6 cut(s) 94, 400, 957, 1414, 1573, 1654
BstMBI GATC 4 cut(s) 669, 841, 1328, 1465
BstMWI GCNNNNNNNGC 5 cut(s) 32, 977, 980, 986, 1652
BstNI CCWGG 5 cut(s) 86, 839, 870, 878, 1522
BstNSI RCATGY 5 cut(s) 145, 335, 770, 791, 1708
BstPAI GACNNNNGTC 1 cut(s) 1767
BstPI GGTNACC 2 cut(s) 394, 872
BstSCI CCNGG 5 cut(s) 84, 837, 868, 876, 1520
BstSFI CTRYAG 1 cut(s) 705
BstV1I GCAGC 5 cut(s) 535, 782, 958, 992, 1702
BstV2I GAAGAC 2 cut(s) 266, 621
Bsu36I CCTNAGG 2 cut(s) 95, 1443
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 3 cut(s) 989, 1440, 1776
BtgI CCRYGG 1 cut(s) 1563
BtrI CACGTC 1 cut(s) 1244
BtsCI GGATG 5 cut(s) 342, 535, 797, 920, 1503
BtsI GCAGTG 3 cut(s) 1057, 1614, 1762
BtsIMutI CAGTG 7 cut(s) 36, 483, 1057, 1183, 1614, 1717, 1762
Cac8I GCNNGC 5 cut(s) 59, 143, 517, 768, 1805
CciI TCATGA 3 cut(s) 504, 832, 1360
Cfr13I GGNCC 3 cut(s) 181, 901, 1439
CseI GACGC 1 cut(s) 260
CsiI ACCWGGT 2 cut(s) 868, 1520
Csp6I GTAC 2 cut(s) 1373, 1674
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviQI GTAC 2 cut(s) 1373, 1674
DdeI CTNAG 6 cut(s) 95, 822, 1123, 1317, 1376, 1443
DpnI GATC 4 cut(s) 671, 843, 1330, 1467
DpnII GATC 4 cut(s) 669, 841, 1328, 1465
DraI TTTAAA 1 cut(s) 124
DraIII CACNNNGTG 1 cut(s) 1682
DriI GACNNNNNGTC 2 cut(s) 479, 1522
EaeI YGGCCR 1 cut(s) 1774
Eam1104I CTCTTC 6 cut(s) 300, 660, 927, 1417, 1496, 1775
Eam1105I GACNNNNNGTC 2 cut(s) 479, 1522
EarI CTCTTC 6 cut(s) 300, 660, 927, 1417, 1496, 1775
Eco130I CCWWGG 2 cut(s) 381, 1563
Eco31I GGTCTC 3 cut(s) 94, 957, 1573
Eco47I GGWCC 2 cut(s) 181, 901
Eco57I CTGAAG 5 cut(s) 216, 281, 1218, 1362, 1758
Eco81I CCTNAGG 2 cut(s) 95, 1443
Eco91I GGTNACC 2 cut(s) 394, 872
EcoO109I RGGNCCY 1 cut(s) 901
EcoO65I GGTNACC 2 cut(s) 394, 872
EcoRI GAATTC 2 cut(s) 629, 1167
EcoRII CCWGG 5 cut(s) 84, 837, 868, 876, 1520
EcoT14I CCWWGG 2 cut(s) 381, 1563
EcoT22I ATGCAT 3 cut(s) 147, 612, 1489
ErhI CCWWGG 2 cut(s) 381, 1563
FalI AAGNNNNNCTT 4 cut(s) 249, 281, 353, 385
FaqI GGGAC 2 cut(s) 473, 1045
FauNDI CATATG 1 cut(s) 1489
FblI GTMKAC 4 cut(s) 213, 711, 1729, 1790
Fnu4HI GCNGC 5 cut(s) 549, 771, 972, 981, 1716
FokI GGATG 5 cut(s) 329, 522, 784, 907, 1510
Fsp4HI GCNGC 5 cut(s) 549, 771, 972, 981, 1716
FspBI CTAG 6 cut(s) 495, 512, 738, 995, 1131, 1247
GluI GCNGC 5 cut(s) 549, 771, 972, 981, 1716
GsaI CCCAGC 1 cut(s) 464
HaeIII GGCC 3 cut(s) 989, 1440, 1776
HgaI GACGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 1457
HincII GTYRAC 5 cut(s) 214, 778, 1239, 1554, 1730
HindII GTYRAC 5 cut(s) 214, 778, 1239, 1554, 1730
HinfI GANTC 8 cut(s) 11, 65, 690, 1190, 1310, 1477, 1555, 1628
HphI GGTGA 9 cut(s) 102, 314, 345, 406, 449, 1407, 1475, 1694, 1772
Hpy188III TCNNGA 8 cut(s) 172, 505, 833, 1361, 1469, 1481, 1595, 1625
HpyAV CCTTC 7 cut(s) 317, 475, 608, 724, 1227, 1337, 1733
HpyCH4III ACNGT 9 cut(s) 487, 544, 709, 1058, 1150, 1178, 1673, 1727, 1818
HpyCH4IV ACGT 2 cut(s) 1243, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 977, 980, 986, 1652
HpyF3I CTNAG 6 cut(s) 95, 822, 1123, 1317, 1376, 1443
HpySE526I ACGT 2 cut(s) 1243, 1457
Hsp92I GRCGYC 1 cut(s) 1457
Kzo9I GATC 4 cut(s) 669, 841, 1328, 1465
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 2 cut(s) 174, 295
Lsp1109I GCAGC 5 cut(s) 535, 782, 958, 992, 1702
LweI GCATC 5 cut(s) 367, 422, 534, 806, 1474
MabI ACCWGGT 2 cut(s) 868, 1520
MaeI CTAG 6 cut(s) 495, 512, 738, 995, 1131, 1247
MaeII ACGT 2 cut(s) 1243, 1457
MalI GATC 4 cut(s) 671, 843, 1330, 1467
MboI GATC 4 cut(s) 669, 841, 1328, 1465
MfeI CAATTG 2 cut(s) 114, 845
MlsI TGGCCA 1 cut(s) 1776
MluNI TGGCCA 1 cut(s) 1776
MlyI GAGTC 4 cut(s) 5, 699, 1319, 1549
MmeI TCCRAC 2 cut(s) 563, 1278
Mox20I TGGCCA 1 cut(s) 1776
Mph1103I ATGCAT 3 cut(s) 147, 612, 1489
MscI TGGCCA 1 cut(s) 1776
MseI TTAA 9 cut(s) 123, 360, 447, 567, 1211, 1220, 1287, 1832, 1870
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 1776
MspA1I CMGCKG 1 cut(s) 773
MspR9I CCNGG 5 cut(s) 86, 839, 870, 878, 1522
MunI CAATTG 2 cut(s) 114, 845
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 5 cut(s) 86, 839, 870, 878, 1522
MwoI GCNNNNNNNGC 5 cut(s) 32, 977, 980, 986, 1652
NcoI CCATGG 1 cut(s) 1563
NdeI CATATG 1 cut(s) 1489
NdeII GATC 4 cut(s) 669, 841, 1328, 1465
NlaIV GGNNCC 2 cut(s) 170, 903
NmuCI GTSAC 7 cut(s) 108, 394, 481, 691, 999, 1052, 1760
NsiI ATGCAT 3 cut(s) 147, 612, 1489
NspI RCATGY 5 cut(s) 145, 335, 770, 791, 1708
PaeI GCATGC 2 cut(s) 145, 770
PagI TCATGA 3 cut(s) 504, 832, 1360
PciI ACATGT 2 cut(s) 787, 1704
PciSI GCTCTTC 1 cut(s) 927
PctI GAATGC 1 cut(s) 937
PfeI GAWTC 4 cut(s) 65, 1190, 1477, 1628
PflMI CCANNNNNTGG 2 cut(s) 1109, 1609
PkrI GCNGC 5 cut(s) 550, 772, 973, 982, 1717
PleI GAGTC 4 cut(s) 5, 698, 1318, 1549
PpsI GAGTC 4 cut(s) 5, 698, 1318, 1549
PpuMI RGGWCCY 1 cut(s) 901
PscI ACATGT 2 cut(s) 787, 1704
PshAI GACNNNNGTC 1 cut(s) 1767
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 5 cut(s) 84, 837, 868, 876, 1520
PspEI GGTNACC 2 cut(s) 394, 872
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 5 cut(s) 84, 837, 868, 876, 1520
PspN4I GGNNCC 2 cut(s) 170, 903
PspPI GGNCC 3 cut(s) 181, 901, 1439
PspPPI RGGWCCY 1 cut(s) 901
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 2 cut(s) 1374, 1675
RsaNI GTAC 2 cut(s) 1373, 1674
RseI CAYNNNNRTG 1 cut(s) 372
SalI GTCGAC 2 cut(s) 212, 1728
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 9 cut(s) 123, 360, 447, 567, 1211, 1220, 1287, 1832, 1870
SatI GCNGC 5 cut(s) 549, 771, 972, 981, 1716
Sau3AI GATC 4 cut(s) 669, 841, 1328, 1465
Sau96I GGNCC 3 cut(s) 181, 901, 1439
ScaI AGTACT 1 cut(s) 1374
SchI GAGTC 4 cut(s) 5, 699, 1319, 1549
ScrFI CCNGG 5 cut(s) 86, 839, 870, 878, 1522
SexAI ACCWGGT 2 cut(s) 868, 1520
SfaNI GCATC 5 cut(s) 367, 422, 534, 806, 1474
SfcI CTRYAG 1 cut(s) 705
SinI GGWCC 2 cut(s) 181, 901
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 1469
SmoI CTYRAG 1 cut(s) 1469
SphI GCATGC 2 cut(s) 145, 770
SsiI CCGC 1 cut(s) 26
SspMI CTAG 6 cut(s) 495, 512, 738, 995, 1131, 1247
StyD4I CCNGG 5 cut(s) 84, 837, 868, 876, 1520
StyI CCWWGG 2 cut(s) 381, 1563
TaaI ACNGT 9 cut(s) 487, 544, 709, 1058, 1150, 1178, 1673, 1727, 1818
TaiI ACGT 2 cut(s) 1246, 1460
TaqI TCGA 4 cut(s) 213, 633, 1206, 1729
TatI WGTACW 2 cut(s) 1372, 1673
TfiI GAWTC 4 cut(s) 65, 1190, 1477, 1628
Tru1I TTAA 9 cut(s) 123, 360, 447, 567, 1211, 1220, 1287, 1832, 1870
Tru9I TTAA 9 cut(s) 123, 360, 447, 567, 1211, 1220, 1287, 1832, 1870
TscAI CASTG 7 cut(s) 43, 490, 1057, 1183, 1614, 1717, 1762
TseFI GTSAC 7 cut(s) 108, 394, 481, 691, 999, 1052, 1760
TseI GCWGC 5 cut(s) 548, 770, 971, 980, 1715
Tsp45I GTSAC 7 cut(s) 108, 394, 481, 691, 999, 1052, 1760
TspDTI ATGAA 8 cut(s) 17, 521, 627, 714, 1349, 1377, 1412, 1514
TspRI CASTG 7 cut(s) 43, 490, 1057, 1183, 1614, 1717, 1762
Van91I CCANNNNNTGG 2 cut(s) 1109, 1609
VpaK11BI GGWCC 2 cut(s) 181, 901
XapI RAATTY 3 cut(s) 125, 629, 1167
XceI RCATGY 5 cut(s) 145, 335, 770, 791, 1708
XmiI GTMKAC 4 cut(s) 213, 711, 1729, 1790
XspI CTAG 6 cut(s) 495, 512, 738, 995, 1131, 1247
ZraI GACGTC 1 cut(s) 1458
ZrmI AGTACT 1 cut(s) 1374
Zsp2I ATGCAT 3 cut(s) 147, 612, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.