Rorug03G0084700

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0006939
Physical Location & Seq
Reverse (-)
6548212 .. 6551368
3157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0084700.1

Sequence Viewer

Length: 1803 bp
ATGACGTTGGAGTTTGGCAAAACTATTGATGAAGATGTGGTGGAGCAAATCCTATCAACTCTGCCACCCAAATCTCTTAAGCGATTCCAGTGCGTCTCTAATAGGTGGTATGCTCTGATCACCACTCCCAGGTTCGTAGCTAAGCACCTCTCCATTTCCATGCACAACAATCTCTCCACCAGTGTTCTTATGAAACGTAAAGTCCATAAGGACACCAACTCTGACGAGACTCAAGAGTTGTTCTCATTTCTTCATTTTCGAAATGATGAAGATAATGATGTTGATGATGTGCATGATGAGCATAGCTTTCTTTCTAGTATCCAGGAATTCCATATTCCGTTTTCTACCGGTGTAAAGACTCGGGCCGAATCACTTATAATTATAGGCCATTGTAACGGGATCATTTGTCTAGCTCAATCAGTCTCTGGTGAGGTGATTATTTGCAACCCAGCAATTCATGAATATAAGCTTCTTCCCCCCTCTCCGTACCTTCCAGATTCCGATTTAGAGATGGATTGGGATTTGGATATGATCCGAACTTTAACGAATATAAAATTGTTAACATTGGATTTCCTGCTCCAGAATTATCTACGCCTGATGGATTTAACATTTATAATCCTCCCCAAGCAGCTGTCTACACCCTGGATGCACTTCAAGGATATGTGTTTTTGGCGAGCACCTGAGCAACACAAGGAATTGGATGTGCTTGATGAAGACGAGGAGCAATTCATTAGGGAAGTTATCATTATGTTTGATACTGGGGATGAGCTATTTCATAATATAATGTTACCGGATGAATTTGATTATCCATCAAAAAATTATTTCTTTGAGAGCCTTTTAGTGTGGAAGGACTCCGTTGCTCTTTTGGGAATACAAATCTGTCAACTATCATCATATGGAATATGGGTGATAGATGAATTTGGTGGTCATAACGGTGGTGCTTGGACAAAACACATAACTTTTGAGCTCCCTGTGGAACCGTTGATATTTTGGAAGAGCGACAGGGTTCTTTTAAATGATCCTAACGACACTGATTATAGAGGACTTATATTCTCTTATAATCTCGATACCAAAAAGCTTAAAAATCTTCCCATTCAAAGCGAGCGGACTGACTCTTCTGCTATTGTGTATGTGAGCAGTATAGTTTCAGTATTGGGAGGCAGCAAACCCAAGAACAAAGATAATTCTACACCCAATGCTGAATTTTCTGTATTTGAGTACCCTTCTCCACTAGTCAGTCATTATATGGTTGACAAGAAAACTTACTCATATTCGGTGTGGGCAATCCCGTCAGATGATGTGTCTCTCCGGATTAAGAAAGTGATGGTGGGACTGCGGACTGAATTCGGCGGGCCGGAGATTGATCCCCACATTGTCGTTGTGGGGTCCATTCGTATGAAGCATGAGGATATGCTCACCAAGTTCAGATCTCTCCAGTCTAATGTTATTTCTTCGTACAAAGCAAAAGTTAATCAAGTCGTTACTAGGAGTTCCTATTTCCAATGTATTTCCCTCCTCATTCATTCATCTTTCGAGGTGTCACCTGAGTTATATTTTGCAACAGGAGTTTGTGGTGGACGATTCCAATTTTGTAATAAAGTTAGGCCACATTTGAGCCTCCTTTATGGTTACTTGACAGAAGAAGAGAGGAAGAAAGCTCAAGAAAAAGTCAGTTATCTGGATGAAGGCCTCAGCAGCTTGAGCTTCTTTATAACTCGACTTGCATTGTACAAAATTGACTACAAAGATAGAAGTCTCAAATCTTGGGAGAAGATTGCTGATTACCCCCTCCAATTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

600

Amino Acids

69.26

Weight (kDa)

5.45

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 11 - 45 9e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 377, 614, 1059, 1712
AccBSI CCGCTC 1 cut(s) 1105
AccI GTMKAC 1 cut(s) 635
AccIII TCCGGA 1 cut(s) 1308
AciI CCGC 3 cut(s) 1105, 1336, 1350
AclWI GGATC 4 cut(s) 407, 526, 1013, 1358
AcsI RAATTY 5 cut(s) 326, 797, 917, 1202, 1343
AfaI GTAC 4 cut(s) 488, 1220, 1457, 1730
AfiI CCNNNNNNNGG 1 cut(s) 129
AflII CTTAAG 1 cut(s) 77
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 2 cut(s) 655, 1097
AhlI ACTAGT 1 cut(s) 1231
AjnI CCWGG 3 cut(s) 128, 321, 641
Alw21I GWGCWC 2 cut(s) 679, 969
Alw26I GTCTC 5 cut(s) 100, 221, 427, 1308, 1760
AlwI GGATC 4 cut(s) 407, 526, 1013, 1358
AlwNI CAGNNNCTG 1 cut(s) 425
Ama87I CYCGRG 1 cut(s) 360
Aor13HI TCCGGA 1 cut(s) 1308
AoxI GGCC 5 cut(s) 363, 385, 1352, 1604, 1687
ApeKI GCWGC 3 cut(s) 628, 1161, 1695
ApoI RAATTY 5 cut(s) 326, 797, 917, 1202, 1343
AsiGI ACCGGT 1 cut(s) 347
AspS9I GGNCC 3 cut(s) 363, 1352, 1386
AsuHPI GGTGA 6 cut(s) 112, 440, 445, 919, 1408, 1533
AsuII TTCGAA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 1 cut(s) 1386
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 720
Bbv12I GWGCWC 2 cut(s) 679, 969
BbvCI CCTCAGC 1 cut(s) 1691
BbvI GCAGC 3 cut(s) 640, 1173, 1707
BccI CCATC 4 cut(s) 505, 592, 817, 1318
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 3 cut(s) 130, 323, 643
BciVI GTATCC 1 cut(s) 329
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 5 cut(s) 100, 221, 427, 1308, 1760
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 4 cut(s) 315, 410, 1232, 1485
BfrI CTTAAG 1 cut(s) 77
BfuI GTATCC 1 cut(s) 329
BglII AGATCT 1 cut(s) 1427
BisI GCNGC 3 cut(s) 629, 1162, 1696
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 3 cut(s) 630, 1163, 1697
Bme1390I CCNGG 3 cut(s) 130, 323, 643
Bme18I GGWCC 1 cut(s) 1386
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 363, 1352, 1386
BmiI GGNNCC 2 cut(s) 978, 1387
BmrFI CCNGG 3 cut(s) 130, 323, 643
BmrI ACTGGG 1 cut(s) 768
BmsI GCATC 1 cut(s) 636
BmuI ACTGGG 1 cut(s) 768
BpiI GAAGAC 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 2 cut(s) 563, 1418
Bpu10I CCTNAGC 2 cut(s) 681, 1691
Bpu1102I GCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 3 cut(s) 216, 1644, 1720
BsaJI CCNNGG 2 cut(s) 128, 641
BsaWI WCCGGW 3 cut(s) 347, 790, 1308
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 4 cut(s) 88, 180, 763, 1435
BseAI TCCGGA 1 cut(s) 1308
BseBI CCWGG 3 cut(s) 130, 323, 643
BseDI CCNNGG 2 cut(s) 128, 641
BseGI GGATG 5 cut(s) 651, 706, 769, 799, 1687
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMII CTCAG 3 cut(s) 672, 1536, 1705
BseNI ACTGG 4 cut(s) 88, 180, 763, 1435
BseRI GAGGAG 2 cut(s) 734, 1505
BseXI GCAGC 3 cut(s) 640, 1173, 1707
BseYI CCCAGC 1 cut(s) 448
BshFI GGCC 5 cut(s) 365, 387, 1354, 1606, 1689
BshTI ACCGGT 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 679, 969
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 4 cut(s) 348, 791, 1309, 1355
BslFI GGGAC 1 cut(s) 1344
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 5 cut(s) 100, 221, 427, 1308, 1760
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 1344
BsnI GGCC 5 cut(s) 365, 387, 1354, 1606, 1689
BsoBI CYCGRG 1 cut(s) 360
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 679, 969
Bsp13I TCCGGA 1 cut(s) 1308
Bsp1407I TGTACA 1 cut(s) 1728
Bsp143I GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 3 cut(s) 1105, 1336, 1350
BspANI GGCC 5 cut(s) 365, 387, 1354, 1606, 1689
BspCNI CTCAG 3 cut(s) 673, 1537, 1704
BspEI TCCGGA 1 cut(s) 1308
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 978, 1387
BspPI GGATC 4 cut(s) 407, 526, 1013, 1358
BspQI GCTCTTC 1 cut(s) 989
BspT104I TTCGAA 1 cut(s) 259
BspTI CTTAAG 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 1105
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 1728
BsrI ACTGG 4 cut(s) 88, 180, 763, 1435
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 128, 641
BssMI GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
Bst2UI CCWGG 3 cut(s) 130, 323, 643
Bst4CI ACNGT 2 cut(s) 935, 981
Bst6I CTCTTC 3 cut(s) 989, 1120, 1638
BstAFI CTTAAG 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 1728
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 3 cut(s) 675, 1103, 1352
BstDEI CTNAG 4 cut(s) 141, 681, 1545, 1691
BstF5I GGATG 5 cut(s) 651, 706, 769, 799, 1687
BstKTI GATC 6 cut(s) 120, 402, 534, 1021, 1366, 1430
BstMAI GTCTC 5 cut(s) 100, 221, 427, 1308, 1760
BstMBI GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
BstMWI GCNNNNNNNGC 3 cut(s) 298, 1695, 1701
BstNI CCWGG 3 cut(s) 130, 323, 643
BstSCI CCNGG 3 cut(s) 128, 321, 641
BstV1I GCAGC 3 cut(s) 640, 1173, 1707
BstV2I GAAGAC 1 cut(s) 720
BstX2I RGATCY 1 cut(s) 1427
BstYI RGATCY 1 cut(s) 1427
BsuI GTATCC 1 cut(s) 329
BsuRI GGCC 5 cut(s) 365, 387, 1354, 1606, 1689
BtsCI GGATG 5 cut(s) 651, 706, 769, 799, 1687
BtsIMutI CAGTG 3 cut(s) 95, 187, 1029
Cac8I GCNNGC 3 cut(s) 675, 1103, 1352
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 363, 1352, 1386
CseI GACGC 1 cut(s) 82
Csp6I GTAC 4 cut(s) 487, 1219, 1456, 1729
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 4 cut(s) 160, 293, 458, 1403
CviQI GTAC 4 cut(s) 487, 1219, 1456, 1729
DdeI CTNAG 4 cut(s) 141, 681, 1545, 1691
DpnI GATC 6 cut(s) 119, 401, 533, 1020, 1365, 1429
DpnII GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
DraI TTTAAA 1 cut(s) 1014
Eam1104I CTCTTC 3 cut(s) 989, 1120, 1638
EarI CTCTTC 3 cut(s) 989, 1120, 1638
Ecl136II GAGCTC 1 cut(s) 967
Eco147I AGGCCT 1 cut(s) 1689
Eco24I GRGCYC 1 cut(s) 969
Eco47I GGWCC 1 cut(s) 1386
Eco53kI GAGCTC 1 cut(s) 967
Eco88I CYCGRG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 967
EcoRI GAATTC 2 cut(s) 326, 1343
EcoRII CCWGG 3 cut(s) 128, 321, 641
EcoT38I GRGCYC 1 cut(s) 969
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 4 cut(s) 163, 296, 461, 1406
FaqI GGGAC 1 cut(s) 1344
FatI CATG 4 cut(s) 159, 292, 457, 1402
FauI CCCGC 1 cut(s) 1343
FauNDI CATATG 1 cut(s) 895
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 635
Fnu4HI GCNGC 3 cut(s) 629, 1162, 1696
FokI GGATG 5 cut(s) 658, 713, 776, 806, 1694
FriOI GRGCYC 1 cut(s) 969
Fsp4HI GCNGC 3 cut(s) 629, 1162, 1696
FspBI CTAG 4 cut(s) 315, 410, 1232, 1485
GluI GCNGC 3 cut(s) 629, 1162, 1696
GsaI CCCAGC 1 cut(s) 452
GsuI CTGGAG 2 cut(s) 563, 1418
HaeIII GGCC 5 cut(s) 365, 387, 1354, 1606, 1689
HapII CCGG 4 cut(s) 348, 791, 1309, 1355
HgaI GACGC 1 cut(s) 82
Hin1II CATG 4 cut(s) 163, 296, 461, 1406
HincII GTYRAC 3 cut(s) 561, 884, 1252
HindII GTYRAC 3 cut(s) 561, 884, 1252
HindIII AAGCTT 2 cut(s) 467, 1076
HinfI GANTC 8 cut(s) 84, 229, 358, 368, 497, 851, 1112, 1581
HpaI GTTAAC 1 cut(s) 561
HpaII CCGG 4 cut(s) 348, 791, 1309, 1355
HphI GGTGA 6 cut(s) 112, 440, 445, 919, 1408, 1533
Hpy166II GTNNAC 5 cut(s) 561, 636, 884, 1252, 1577
Hpy188I TCNGA 6 cut(s) 117, 223, 502, 536, 1294, 1427
Hpy188III TCNNGA 8 cut(s) 233, 458, 494, 580, 1064, 1309, 1661, 1679
Hpy8I GTNNAC 5 cut(s) 561, 636, 884, 1252, 1577
HpyAV CCTTC 4 cut(s) 500, 841, 1233, 1679
HpyCH4III ACNGT 2 cut(s) 935, 981
HpyCH4IV ACGT 2 cut(s) 5, 196
HpyCH4V TGCA 6 cut(s) 163, 292, 444, 649, 1559, 1724
HpyF10VI GCNNNNNNNGC 3 cut(s) 298, 1695, 1701
HpyF3I CTNAG 4 cut(s) 141, 681, 1545, 1691
HpySE526I ACGT 2 cut(s) 5, 196
Hsp92II CATG 4 cut(s) 163, 296, 461, 1406
Kpn2I TCCGGA 1 cut(s) 1308
Ksp22I TGATCA 1 cut(s) 117
KspAI GTTAAC 1 cut(s) 561
Kzo9I GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
LguI GCTCTTC 1 cut(s) 989
LmnI GCTCC 4 cut(s) 43, 582, 721, 972
Lsp1109I GCAGC 3 cut(s) 640, 1173, 1707
LweI GCATC 1 cut(s) 636
MaeI CTAG 4 cut(s) 315, 410, 1232, 1485
MaeII ACGT 2 cut(s) 5, 196
MaeIII GTNAC 5 cut(s) 392, 786, 1480, 1539, 1628
MalI GATC 6 cut(s) 119, 401, 533, 1020, 1365, 1429
MbiI CCGCTC 1 cut(s) 1105
MboI GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
MflI RGATCY 1 cut(s) 1427
MhlI GDGCHC 2 cut(s) 679, 969
MlyI GAGTC 4 cut(s) 223, 352, 845, 1106
MroI TCCGGA 1 cut(s) 1308
MseI TTAA 8 cut(s) 78, 542, 560, 605, 1013, 1080, 1314, 1470
MslI CAYNNNNRTG 3 cut(s) 158, 933, 1394
MspA1I CMGCKG 1 cut(s) 631
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 4 cut(s) 348, 791, 1309, 1355
MspR9I CCNGG 3 cut(s) 130, 323, 643
MvaI CCWGG 3 cut(s) 130, 323, 643
MwoI GCNNNNNNNGC 3 cut(s) 298, 1695, 1701
NdeI CATATG 1 cut(s) 895
NdeII GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
NlaIII CATG 4 cut(s) 163, 296, 461, 1406
NlaIV GGNNCC 2 cut(s) 978, 1387
NmuCI GTSAC 1 cut(s) 1539
NspV TTCGAA 1 cut(s) 259
PagI TCATGA 1 cut(s) 457
PceI AGGCCT 1 cut(s) 1689
PciSI GCTCTTC 1 cut(s) 989
PfeI GAWTC 4 cut(s) 84, 368, 497, 1581
PfoI TCCNGGA 1 cut(s) 321
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 3 cut(s) 630, 1163, 1697
PleI GAGTC 4 cut(s) 223, 352, 845, 1106
PpsI GAGTC 4 cut(s) 223, 352, 845, 1106
PsiI TTATAA 4 cut(s) 377, 614, 1059, 1712
Psp124BI GAGCTC 1 cut(s) 969
Psp6I CCWGG 3 cut(s) 128, 321, 641
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 3 cut(s) 128, 321, 641
PspN4I GGNNCC 2 cut(s) 978, 1387
PspPI GGNCC 3 cut(s) 363, 1352, 1386
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 1427
PvuII CAGCTG 1 cut(s) 631
RsaI GTAC 4 cut(s) 488, 1220, 1457, 1730
RsaNI GTAC 4 cut(s) 487, 1219, 1456, 1729
RseI CAYNNNNRTG 3 cut(s) 158, 933, 1394
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 989
SaqAI TTAA 8 cut(s) 78, 542, 560, 605, 1013, 1080, 1314, 1470
SatI GCNGC 3 cut(s) 629, 1162, 1696
Sau3AI GATC 6 cut(s) 117, 399, 531, 1018, 1363, 1427
Sau96I GGNCC 3 cut(s) 363, 1352, 1386
SchI GAGTC 4 cut(s) 223, 352, 845, 1106
ScrFI CCNGG 3 cut(s) 130, 323, 643
SduI GDGCHC 2 cut(s) 679, 969
SfaNI GCATC 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 1 cut(s) 1386
SmiMI CAYNNNNRTG 3 cut(s) 158, 933, 1394
SmlI CTYRAG 4 cut(s) 77, 231, 1659, 1699
SmoI CTYRAG 4 cut(s) 77, 231, 1659, 1699
SpeI ACTAGT 1 cut(s) 1231
SseBI AGGCCT 1 cut(s) 1689
SsiI CCGC 3 cut(s) 1105, 1336, 1350
SspMI CTAG 4 cut(s) 315, 410, 1232, 1485
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 1 cut(s) 1689
StyD4I CCNGG 3 cut(s) 128, 321, 641
TaaI ACNGT 2 cut(s) 935, 981
TaiI ACGT 2 cut(s) 8, 199
TaqI TCGA 4 cut(s) 259, 1065, 1533, 1717
TatI WGTACW 1 cut(s) 1728
TfiI GAWTC 4 cut(s) 84, 368, 497, 1581
Tru1I TTAA 8 cut(s) 78, 542, 560, 605, 1013, 1080, 1314, 1470
Tru9I TTAA 8 cut(s) 78, 542, 560, 605, 1013, 1080, 1314, 1470
TscAI CASTG 3 cut(s) 95, 187, 1036
TseFI GTSAC 1 cut(s) 1539
TseI GCWGC 3 cut(s) 628, 1161, 1695
Tsp45I GTSAC 1 cut(s) 1539
TspGWI ACGGA 3 cut(s) 327, 474, 844
TspRI CASTG 3 cut(s) 95, 187, 1036
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 1386
XapI RAATTY 5 cut(s) 326, 797, 917, 1202, 1343
XmiI GTMKAC 1 cut(s) 635
XspI CTAG 4 cut(s) 315, 410, 1232, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.