Rh3AG132600

SAWADEE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0006939
Physical Location & Seq
Forward (+)
11461869 .. 11465705
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG132600.1

Sequence Viewer

Length: 1743 bp
ATGAAAATGGACTCTATCAGAGAAGCGGAGAGTGCCACTGAACTTGAAGCCTTGTGCAAGCAGGATTCTTCTTGGTATCCCTGCCAGGTTTCCCTCAGCTCTACTGAAGATAGCCTAATTGTCGACTTTGGGAGGCAGGAGTTACAAGATATGGTGTTGAACAAGGATGAAGCACTTACGCGTCTTCGTTTTCGTTCGGCTCCTCTTCAGGGTGACGACTGCTCTCGCATTGAAGGAGAGCATGTTCTTTCCATCCATAAATCACCATATAAGAGCCACTTATATGATGCCAAGGTGGAAAAGGTGACCAGAGTGAGACACTCTACAAGGGTCTATTGCAGATGCAGTTTTATGATTAAGTGGCTTCACCCAGACTTCAAAGGACAGATGGTCACTGTCCCCTCTAGTTCAATCATGAAACTAGCAAGCAAAAGCATCAATTCTCATCCAACTGTTGCTGCATTATTGAAATCAGTTAAGCAAATGGGTTTATATACTGCACCTCCCCTTCCAATCATGCATGAAGACAATGATGTTGAATTCGACCTCAATAAATTATTGGGAAAGCAAATAGAAGAGATCAACATTTCAGCTAATGGAGTCACAAATGAAACTACAGTAGACATATTAGAGGTGGTTAAAGCTGTTGACAATGGACATGTTACTTTGTCCAAAGTAGGCACTTCAAAAGCTCAGGTTTCTCATGACCAGGATCAATTGAAATCTGTTAGTAACAGACCTGGTAACCTGGAAGTAAACATAGAAGATGAGGACCCACAAGCCCCTCTCTCATCCAAACAAGAAGAGCATTCAGAACACAGGTCTCATATTAGTCCTCTTGCTGCCCGTGCAGCTCTGGCCTCCCTAGTGTCGCTTACTCACAAGCATATTGCTATAAGTGGGACTGAACTTTTCAAGTCCAGTGACAGTAATAATATGTCTATTATGGTATCTTCTACTGCAATCAAATCTCCAAAAAATGGAAATGCGAACTCAGGTGTTAGAACAACTCGCTCAACAGTTCAGAAAGGTTTTGGAATTCAGAACAGTGATTTGCATGATTTTACTGAAGCCATCGAGTTAAGGGTCTTAACCAACACAGGAAGGTTGACACATTTAGCAGTCAAAGAAGAAAAAGATATATCATCTATGGAAATTAAACAAGGGTTGGAAGAAAGTGAGTCTGCTCAGAGTACAGGATCATATTCTTCTGAAGGAACTGACATTGTTCATGAAAGCAAAGTACTCAGAAAGAAGAATGATATATCAAACAAAGCAGTCTCTTCACCATTTCATTCAGAGAGTAACGGCCCTAAGGAAAATCTGACGTCTGGTGATCTTGAGGCGATTCAAGATGCATATGTTCAGATGAAGACTTGTGCTAAAGACACCAAGTCAAGTGTTTCAACCAACATGAGGAGGTTGACTCGTTCCATGGTCTCTTGTAAAGACAATTTGATAGTTCCTGAAAGCCATGCAGTGGAAAAGGAAAATCGTGAATCAAAAAAGAAGAAAGCTGTCTCTGCGACATCTCATAATGACAGTACACAAGGTGAAGAAAGTAATAGAAAGCACATGTCCGGTGCTGTCAGAAACAGTCGACAAACTGAAGGAAAAATGTCAGGCAGTGGTGACAATAGTCAAGGCCAGAAGAGGAAGTGTACTTCTTTGAGTAGGCAAGAGCAACGGTTTTCTCCACGATTAAGGGTTCTCCCTCGCACTCGTTCACAAAACAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAWADEE

Protein Analysis

580

Amino Acids

64.13

Weight (kDa)

8.43

Isoelectric Point (pI)

55.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAWADEE PF16719 13 - 139 1.4e-22 SAWADEE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1331
AccB7I CCANNNNNTGG 2 cut(s) 980, 1480
AccI GTMKAC 3 cut(s) 123, 621, 1600
AccII CGCG 1 cut(s) 181
AciI CCGC 1 cut(s) 26
AclWI GGATC 2 cut(s) 720, 1207
AcsI RAATTY 2 cut(s) 539, 1038
AcuI CTGAAG 5 cut(s) 126, 191, 1089, 1233, 1629
AcyI GRCGYC 1 cut(s) 1328
AdeI CACNNNGTG 1 cut(s) 1553
AfaI GTAC 4 cut(s) 1195, 1245, 1546, 1663
AfiI CCNNNNNNNGG 4 cut(s) 209, 980, 1416, 1480
AflIII ACRYGT 3 cut(s) 179, 658, 1575
AhdI GACNNNNNGTC 2 cut(s) 389, 1393
AjnI CCWGG 4 cut(s) 84, 708, 739, 747
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 6 cut(s) 99, 593, 644, 692, 854, 1517
AluI AGCT 6 cut(s) 99, 593, 644, 692, 854, 1517
Alw26I GTCTC 5 cut(s) 310, 828, 1285, 1444, 1525
AlwI GGATC 2 cut(s) 720, 1207
AoxI GGCC 3 cut(s) 858, 1309, 1645
ApeKI GCWGC 3 cut(s) 458, 842, 851
ApoI RAATTY 2 cut(s) 539, 1038
AspS9I GGNCC 2 cut(s) 772, 1310
AsuHPI GGTGA 8 cut(s) 224, 255, 316, 359, 1278, 1346, 1565, 1643
AvaII GGWCC 1 cut(s) 772
AxyI CCTNAGG 1 cut(s) 1314
BbsI GAAGAC 3 cut(s) 176, 531, 1379
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 445, 829, 863
BccI CCATC 3 cut(s) 260, 382, 1082
BceAI ACGGC 1 cut(s) 1324
BciT130I CCWGG 4 cut(s) 86, 710, 741, 749
BciVI GTATCC 1 cut(s) 87
BcoDI GTCTC 5 cut(s) 310, 828, 1285, 1444, 1525
BfaI CTAG 3 cut(s) 405, 422, 866
BfmI CTRYAG 1 cut(s) 615
BfuI GTATCC 1 cut(s) 87
BisI GCNGC 3 cut(s) 459, 843, 852
BlsI GCNGC 3 cut(s) 460, 844, 853
BmcAI AGTACT 1 cut(s) 1245
Bme1390I CCNGG 4 cut(s) 86, 710, 741, 749
Bme18I GGWCC 1 cut(s) 772
BmeRI GACNNNNNGTC 2 cut(s) 389, 1393
BmgT120I GGNCC 2 cut(s) 772, 1310
BmiI GGNNCC 2 cut(s) 201, 774
BmrFI CCNGG 4 cut(s) 86, 710, 741, 749
BmsI GCATC 4 cut(s) 277, 332, 444, 1345
BoxI GACNNNNGTC 1 cut(s) 1638
BpiI GAAGAC 3 cut(s) 176, 531, 1379
BplI GAGNNNNNCTC 2 cut(s) 1411, 1443
Bpu10I CCTNAGC 2 cut(s) 95, 693
BpuEI CTTGAG 1 cut(s) 1361
BsaHI GRCGYC 1 cut(s) 1328
BsaI GGTCTC 2 cut(s) 828, 1444
BsaJI CCNNGG 2 cut(s) 291, 1434
BsaWI WCCGGW 1 cut(s) 1580
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 487, 517
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 980, 1416, 1480
Bse1I ACTGG 1 cut(s) 921
Bse21I CCTNAGG 1 cut(s) 1314
BseBI CCWGG 4 cut(s) 86, 710, 741, 749
BseDI CCNNGG 2 cut(s) 291, 1434
BseGI GGATG 4 cut(s) 172, 252, 445, 791
BseLI CCNNNNNNNGG 4 cut(s) 209, 980, 1416, 1480
BseMII CTCAG 5 cut(s) 109, 707, 1008, 1202, 1261
BseNI ACTGG 1 cut(s) 921
BseRI GAGGAG 2 cut(s) 192, 1432
BseXI GCAGC 3 cut(s) 445, 829, 863
BsgI GTGCAG 2 cut(s) 483, 870
Bsh1236I CGCG 1 cut(s) 181
BshFI GGCC 3 cut(s) 860, 1311, 1647
BsiSI CCGG 1 cut(s) 1581
BslFI GGGAC 2 cut(s) 383, 916
BslI CCNNNNNNNGG 4 cut(s) 209, 980, 1416, 1480
BsmAI GTCTC 5 cut(s) 310, 828, 1285, 1444, 1525
BsmFI GGGAC 2 cut(s) 383, 916
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 3 cut(s) 860, 1311, 1647
Bso31I GGTCTC 2 cut(s) 828, 1444
Bsp143I GATC 4 cut(s) 579, 712, 1199, 1336
Bsp19I CCATGG 1 cut(s) 1434
BspACI CCGC 1 cut(s) 26
BspANI GGCC 3 cut(s) 860, 1311, 1647
BspCNI CTCAG 5 cut(s) 108, 706, 1007, 1201, 1260
BspFNI CGCG 1 cut(s) 181
BspHI TCATGA 3 cut(s) 414, 703, 1231
BspLI GGNNCC 2 cut(s) 201, 774
BspPI GGATC 2 cut(s) 720, 1207
BspQI GCTCTTC 1 cut(s) 798
BspTNI GGTCTC 2 cut(s) 828, 1444
BsrI ACTGG 1 cut(s) 921
BssECI CCNNGG 2 cut(s) 291, 1434
BssMI GATC 4 cut(s) 579, 712, 1199, 1336
BssNI GRCGYC 1 cut(s) 1328
BssT1I CCWWGG 2 cut(s) 291, 1434
Bst2UI CCWGG 4 cut(s) 86, 710, 741, 749
Bst4CI ACNGT 9 cut(s) 397, 454, 619, 929, 1021, 1049, 1544, 1598, 1689
Bst6I CTCTTC 5 cut(s) 210, 570, 798, 1288, 1646
BstACI GRCGYC 1 cut(s) 1328
BstC8I GCNNGC 2 cut(s) 59, 427
BstDEI CTNAG 6 cut(s) 95, 693, 994, 1188, 1247, 1314
BstDSI CCRYGG 1 cut(s) 1434
BstEII GGTNACC 2 cut(s) 304, 743
BstF5I GGATG 4 cut(s) 172, 252, 445, 791
BstFNI CGCG 1 cut(s) 181
BstKTI GATC 4 cut(s) 582, 715, 1202, 1339
BstMAI GTCTC 5 cut(s) 310, 828, 1285, 1444, 1525
BstMBI GATC 4 cut(s) 579, 712, 1199, 1336
BstMWI GCNNNNNNNGC 5 cut(s) 32, 848, 851, 857, 1523
BstNI CCWGG 4 cut(s) 86, 710, 741, 749
BstNSI RCATGY 3 cut(s) 245, 662, 1579
BstPAI GACNNNNGTC 1 cut(s) 1638
BstPI GGTNACC 2 cut(s) 304, 743
BstSCI CCNGG 4 cut(s) 84, 708, 739, 747
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 1 cut(s) 181
BstV1I GCAGC 3 cut(s) 445, 829, 863
BstV2I GAAGAC 3 cut(s) 176, 531, 1379
Bsu36I CCTNAGG 1 cut(s) 1314
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 3 cut(s) 860, 1311, 1647
BtgI CCRYGG 1 cut(s) 1434
BtsCI GGATG 4 cut(s) 172, 252, 445, 791
BtsI GCAGTG 2 cut(s) 1485, 1633
BtsIMutI CAGTG 6 cut(s) 36, 393, 928, 1054, 1485, 1633
Cac8I GCNNGC 2 cut(s) 59, 427
CciI TCATGA 3 cut(s) 414, 703, 1231
Cfr13I GGNCC 2 cut(s) 772, 1310
CseI GACGC 1 cut(s) 170
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 4 cut(s) 1194, 1244, 1545, 1662
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviQI GTAC 4 cut(s) 1194, 1244, 1545, 1662
DdeI CTNAG 6 cut(s) 95, 693, 994, 1188, 1247, 1314
DpnI GATC 4 cut(s) 581, 714, 1201, 1338
DpnII GATC 4 cut(s) 579, 712, 1199, 1336
DraIII CACNNNGTG 1 cut(s) 1553
DriI GACNNNNNGTC 2 cut(s) 389, 1393
Eam1104I CTCTTC 5 cut(s) 210, 570, 798, 1288, 1646
Eam1105I GACNNNNNGTC 2 cut(s) 389, 1393
EarI CTCTTC 5 cut(s) 210, 570, 798, 1288, 1646
Eco130I CCWWGG 2 cut(s) 291, 1434
Eco31I GGTCTC 2 cut(s) 828, 1444
Eco47I GGWCC 1 cut(s) 772
Eco57I CTGAAG 5 cut(s) 126, 191, 1089, 1233, 1629
Eco81I CCTNAGG 1 cut(s) 1314
Eco91I GGTNACC 2 cut(s) 304, 743
EcoO109I RGGNCCY 1 cut(s) 772
EcoO65I GGTNACC 2 cut(s) 304, 743
EcoRI GAATTC 2 cut(s) 539, 1038
EcoRII CCWGG 4 cut(s) 84, 708, 739, 747
EcoT14I CCWWGG 2 cut(s) 291, 1434
EcoT22I ATGCAT 2 cut(s) 522, 1360
ErhI CCWWGG 2 cut(s) 291, 1434
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 383, 916
FauNDI CATATG 1 cut(s) 1360
FblI GTMKAC 3 cut(s) 123, 621, 1600
Fnu4HI GCNGC 3 cut(s) 459, 843, 852
FokI GGATG 4 cut(s) 179, 239, 432, 778
Fsp4HI GCNGC 3 cut(s) 459, 843, 852
FspBI CTAG 3 cut(s) 405, 422, 866
GluI GCNGC 3 cut(s) 459, 843, 852
HaeIII GGCC 3 cut(s) 860, 1311, 1647
HapII CCGG 1 cut(s) 1581
HgaI GACGC 1 cut(s) 170
Hin1I GRCGYC 1 cut(s) 1328
HincII GTYRAC 5 cut(s) 124, 649, 1110, 1425, 1601
HindII GTYRAC 5 cut(s) 124, 649, 1110, 1425, 1601
HinfI GANTC 7 cut(s) 11, 65, 600, 1181, 1348, 1426, 1499
HpaII CCGG 1 cut(s) 1581
HphI GGTGA 8 cut(s) 224, 255, 316, 359, 1278, 1346, 1565, 1643
Hpy188III TCNNGA 7 cut(s) 415, 704, 1232, 1340, 1352, 1466, 1496
HpyAV CCTTC 5 cut(s) 227, 518, 1098, 1208, 1604
HpyCH4III ACNGT 9 cut(s) 397, 454, 619, 929, 1021, 1049, 1544, 1598, 1689
HpyCH4IV ACGT 1 cut(s) 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 848, 851, 857, 1523
HpyF3I CTNAG 6 cut(s) 95, 693, 994, 1188, 1247, 1314
HpySE526I ACGT 1 cut(s) 1328
Hsp92I GRCGYC 1 cut(s) 1328
Kzo9I GATC 4 cut(s) 579, 712, 1199, 1336
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 1 cut(s) 205
Lsp1109I GCAGC 3 cut(s) 445, 829, 863
LweI GCATC 4 cut(s) 277, 332, 444, 1345
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 3 cut(s) 405, 422, 866
MaeII ACGT 1 cut(s) 1328
MalI GATC 4 cut(s) 581, 714, 1201, 1338
MboI GATC 4 cut(s) 579, 712, 1199, 1336
MfeI CAATTG 1 cut(s) 716
MluCI AATT 8 cut(s) 117, 439, 539, 554, 716, 1038, 1155, 1453
MluI ACGCGT 1 cut(s) 179
MlyI GAGTC 4 cut(s) 5, 609, 1190, 1420
MmeI TCCRAC 2 cut(s) 473, 1149
Mph1103I ATGCAT 2 cut(s) 522, 1360
MseI TTAA 8 cut(s) 357, 477, 639, 1082, 1091, 1158, 1703, 1741
MslI CAYNNNNRTG 1 cut(s) 282
MspI CCGG 1 cut(s) 1581
MspR9I CCNGG 4 cut(s) 86, 710, 741, 749
MunI CAATTG 1 cut(s) 716
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 4 cut(s) 86, 710, 741, 749
MvnI CGCG 1 cut(s) 181
MwoI GCNNNNNNNGC 5 cut(s) 32, 848, 851, 857, 1523
NcoI CCATGG 1 cut(s) 1434
NdeI CATATG 1 cut(s) 1360
NdeII GATC 4 cut(s) 579, 712, 1199, 1336
NlaIV GGNNCC 2 cut(s) 201, 774
NmuCI GTSAC 6 cut(s) 212, 304, 391, 601, 923, 1631
NsiI ATGCAT 2 cut(s) 522, 1360
NspI RCATGY 3 cut(s) 245, 662, 1579
PagI TCATGA 3 cut(s) 414, 703, 1231
PciI ACATGT 2 cut(s) 658, 1575
PciSI GCTCTTC 1 cut(s) 798
PctI GAATGC 1 cut(s) 808
PfeI GAWTC 3 cut(s) 65, 1348, 1499
PflMI CCANNNNNTGG 2 cut(s) 980, 1480
PkrI GCNGC 3 cut(s) 460, 844, 853
PleI GAGTC 4 cut(s) 5, 608, 1189, 1420
PpsI GAGTC 4 cut(s) 5, 608, 1189, 1420
PpuMI RGGWCCY 1 cut(s) 772
PscI ACATGT 2 cut(s) 658, 1575
PshAI GACNNNNGTC 1 cut(s) 1638
Psp5II RGGWCCY 1 cut(s) 772
Psp6I CCWGG 4 cut(s) 84, 708, 739, 747
PspEI GGTNACC 2 cut(s) 304, 743
PspGI CCWGG 4 cut(s) 84, 708, 739, 747
PspN4I GGNNCC 2 cut(s) 201, 774
PspPI GGNCC 2 cut(s) 772, 1310
PspPPI RGGWCCY 1 cut(s) 772
RsaI GTAC 4 cut(s) 1195, 1245, 1546, 1663
RsaNI GTAC 4 cut(s) 1194, 1244, 1545, 1662
RseI CAYNNNNRTG 1 cut(s) 282
SalI GTCGAC 2 cut(s) 122, 1599
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 8 cut(s) 357, 477, 639, 1082, 1091, 1158, 1703, 1741
SatI GCNGC 3 cut(s) 459, 843, 852
Sau3AI GATC 4 cut(s) 579, 712, 1199, 1336
Sau96I GGNCC 2 cut(s) 772, 1310
ScaI AGTACT 1 cut(s) 1245
SchI GAGTC 4 cut(s) 5, 609, 1190, 1420
ScrFI CCNGG 4 cut(s) 86, 710, 741, 749
SexAI ACCWGGT 1 cut(s) 739
SfaNI GCATC 4 cut(s) 277, 332, 444, 1345
SfcI CTRYAG 1 cut(s) 615
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 1340
SmoI CTYRAG 1 cut(s) 1340
Sse9I AATT 8 cut(s) 117, 439, 539, 554, 716, 1038, 1155, 1453
SsiI CCGC 1 cut(s) 26
SspMI CTAG 3 cut(s) 405, 422, 866
StyD4I CCNGG 4 cut(s) 84, 708, 739, 747
StyI CCWWGG 2 cut(s) 291, 1434
TaaI ACNGT 9 cut(s) 397, 454, 619, 929, 1021, 1049, 1544, 1598, 1689
TaiI ACGT 1 cut(s) 1331
TaqI TCGA 4 cut(s) 123, 543, 1077, 1600
TasI AATT 8 cut(s) 117, 439, 539, 554, 716, 1038, 1155, 1453
TatI WGTACW 4 cut(s) 1193, 1243, 1544, 1661
TfiI GAWTC 3 cut(s) 65, 1348, 1499
Tru1I TTAA 8 cut(s) 357, 477, 639, 1082, 1091, 1158, 1703, 1741
Tru9I TTAA 8 cut(s) 357, 477, 639, 1082, 1091, 1158, 1703, 1741
TscAI CASTG 6 cut(s) 43, 400, 928, 1054, 1485, 1633
TseFI GTSAC 6 cut(s) 212, 304, 391, 601, 923, 1631
TseI GCWGC 3 cut(s) 458, 842, 851
Tsp45I GTSAC 6 cut(s) 212, 304, 391, 601, 923, 1631
TspDTI ATGAA 9 cut(s) 17, 183, 431, 537, 624, 1220, 1248, 1283, 1385
TspRI CASTG 6 cut(s) 43, 400, 928, 1054, 1485, 1633
Van91I CCANNNNNTGG 2 cut(s) 980, 1480
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 2 cut(s) 539, 1038
XceI RCATGY 3 cut(s) 245, 662, 1579
XmiI GTMKAC 3 cut(s) 123, 621, 1600
XspI CTAG 3 cut(s) 405, 422, 866
ZraI GACGTC 1 cut(s) 1329
ZrmI AGTACT 1 cut(s) 1245
Zsp2I ATGCAT 2 cut(s) 522, 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.