FvH4_6g14680

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009024
Physical Location & Seq
Reverse (-)
9034052 .. 9037650
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14680.t2

Sequence Viewer

Length: 1131 bp
ATGGGCAACTCCCACGGCCACCGCAAACACCGTAACGGCGCGAAGCACCCCGTAACGGCCACCCCACCCTTTAAATATCAGTCCTCCGATCATCCCGAAGCTCATAACCCCAGCTCAAGTAAGCCCATCATGGCTGTGTCCTTGCCTTACTCTCATGTTGACTCCTCCCTTCGCGCCCTCGCTGCCCAAGCTGAAGGCTTTGGCCGCTTAGCCATTGGCGGCCTCCATGGTCCTCTTTACCACGTCACCACTCTAGCAGATGATGGTCCAGGGTCACTTAGGGAAGCCTGTCGAAAGAAAGAGCCACTTTGGATTGTGTTTGATATTTCGGGCACTATCAATCTCTCGTCTTACTTGAATGTGTCGTCGCATAAGACAATTGATGGCCGGGGTCAGAGGATTAAGTTTACGGGGAAGGGGTTGAGACTGAAGGAGTGTGAGCATGTGATCATATGCAATTTAGAGTTTGAAGGTGGTAGAGGACCAGATGTTGATGGCATTCAGATAAAACCAAACTCTAAGCACATATGGATAGACCGGTGCAGCCTCCGAGATTATGAAGACGGACTTATAGATATCACCAGATGCAGCACGGATATTACAATTTCAAGGTGTCATTTCTCGGAGCATGATAAGACAATGCTAATTGGAGCAGACCCCTCTCATGTTGATGACAGATGCATCCGAGTTACAATTCACCACTGTTTCTTTGATGGGACCCGACAGCGGCATCCTCGAGTTAGATTCGCCAAAGTACATCTATACAACAATTACACTCGAAACTGGGGCATATATGCTGTTTGTGCCAGTGTAGAATCACAGATATACTCTCAATGCAATATATATGAAGCAGGACAGAAGAAAATAGCCTTTAAATACCTTACAGAGAAGGCAGCGGACAAGGAGGCAGCAACAACCGGCTTCATTAAGTCGGAAGGGGACTTATTCATAACTGGAACTAAAGCAGGATCATCCACCGAGGGTGGTGAAGATTGCACGTTTCATCCTAGCAAGCACTACCAGACATGGACAGTAGGACCTCCAGTCGATGATCTGAAACATGTTCTCCAACATTGCACCGGATGGCAGTCCATTCCTCGACCAGCTGATCAGGCAAGTGTTGCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

377

Amino Acids

41.73

Weight (kDa)

8.38

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 77 - 285 2.3e-29 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 174
AciI CCGC 5 cut(s) 22, 205, 219, 727, 896
AclWI GGATC 1 cut(s) 976
AcoI YGGCCR 4 cut(s) 16, 57, 202, 385
AcuI CTGAAG 2 cut(s) 213, 449
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 2 cut(s) 55, 726
AflIII ACRYGT 1 cut(s) 1060
AgeI ACCGGT 1 cut(s) 537
AgsI TTSAA 3 cut(s) 358, 470, 609
AhdI GACNNNNNGTC 1 cut(s) 1043
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 4 cut(s) 101, 114, 191, 1106
AluI AGCT 4 cut(s) 101, 114, 191, 1106
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 976
Ama87I CYCGRG 1 cut(s) 735
AoxI GGCC 5 cut(s) 16, 57, 202, 220, 385
ApeKI GCWGC 5 cut(s) 182, 543, 588, 893, 908
AsiGI ACCGGT 1 cut(s) 537
AspLEI GCGC 2 cut(s) 41, 176
AspS9I GGNCC 5 cut(s) 230, 266, 482, 717, 1037
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 4 cut(s) 238, 571, 689, 998
AvaI CYCGRG 1 cut(s) 735
AvaII GGWCC 5 cut(s) 230, 266, 482, 717, 1037
BaeGI GKGCMC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 169, 555, 600, 905, 920
BccI CCATC 6 cut(s) 134, 257, 377, 488, 707, 1077
BceAI ACGGC 3 cut(s) 31, 52, 72
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 2 cut(s) 447, 1108
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 2 cut(s) 254, 1008
BisI GCNGC 8 cut(s) 183, 205, 220, 544, 589, 728, 894, 909
BlpI GCTNAGC 1 cut(s) 208
BlsI GCNGC 8 cut(s) 184, 206, 221, 545, 590, 729, 895, 910
Bme1390I CCNGG 2 cut(s) 270, 389
Bme18I GGWCC 5 cut(s) 230, 266, 482, 717, 1037
BmeRI GACNNNNNGTC 1 cut(s) 1043
BmeT110I CYCGRG 1 cut(s) 735
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 5 cut(s) 230, 266, 482, 717, 1037
BmiI GGNNCC 2 cut(s) 718, 719
BmrFI CCNGG 2 cut(s) 270, 389
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 4 cut(s) 575, 668, 690, 739
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 567
BpmI CTGGAG 1 cut(s) 1026
Bpu1102I GCTNAGC 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 100
BpuMI CCSGG 1 cut(s) 389
BsaJI CCNNGG 5 cut(s) 13, 226, 269, 388, 978
BsaWI WCCGGW 2 cut(s) 537, 1079
BsaXI ACNNNNNCTCC 2 cut(s) 1050, 1080
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 726
Bse118I RCCGGY 2 cut(s) 537, 917
Bse1I ACTGG 4 cut(s) 788, 807, 958, 1043
Bse3DI GCAATG 1 cut(s) 1072
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 13, 226, 269, 388, 978
BseGI GGATG 6 cut(s) 91, 681, 730, 971, 1003, 1088
BseLI CCNNNNNNNGG 2 cut(s) 55, 726
BseMI GCAATG 1 cut(s) 1072
BseNI ACTGG 4 cut(s) 788, 807, 958, 1043
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 5 cut(s) 169, 555, 600, 905, 920
BseYI CCCAGC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 41, 174
BshFI GGCC 5 cut(s) 18, 59, 204, 222, 387
BshTI ACCGGT 1 cut(s) 537
BsiHKCI CYCGRG 1 cut(s) 735
BsiSI CCGG 4 cut(s) 388, 538, 918, 1080
BslFI GGGAC 2 cut(s) 730, 953
BslI CCNNNNNNNGG 2 cut(s) 55, 726
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 730, 953
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 5 cut(s) 18, 59, 204, 222, 387
BsoBI CYCGRG 1 cut(s) 735
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 5 cut(s) 88, 447, 968, 1051, 1108
Bsp1720I GCTNAGC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 5 cut(s) 22, 205, 219, 727, 896
BspANI GGCC 5 cut(s) 18, 59, 204, 222, 387
BspFNI CGCG 2 cut(s) 41, 174
BspLI GGNNCC 2 cut(s) 718, 719
BspPI GGATC 1 cut(s) 976
BsrDI GCAATG 1 cut(s) 1072
BsrFI RCCGGY 2 cut(s) 537, 917
BsrI ACTGG 4 cut(s) 788, 807, 958, 1043
BssAI RCCGGY 2 cut(s) 537, 917
BssECI CCNNGG 5 cut(s) 13, 226, 269, 388, 978
BssMI GATC 5 cut(s) 88, 447, 968, 1051, 1108
BssT1I CCWWGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 3 cut(s) 32, 704, 1033
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstC8I GCNNGC 1 cut(s) 1013
BstDEI CTNAG 3 cut(s) 208, 278, 519
BstDSI CCRYGG 2 cut(s) 13, 226
BstF5I GGATG 6 cut(s) 91, 681, 730, 971, 1003, 1088
BstFNI CGCG 2 cut(s) 41, 174
BstHHI GCGC 2 cut(s) 41, 176
BstKTI GATC 5 cut(s) 91, 450, 971, 1054, 1111
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 5 cut(s) 88, 447, 968, 1051, 1108
BstMWI GCNNNNNNNGC 6 cut(s) 182, 188, 204, 803, 1112, 1121
BstNI CCWGG 1 cut(s) 270
BstNSI RCATGY 2 cut(s) 446, 1064
BstSCI CCNGG 2 cut(s) 268, 387
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 2 cut(s) 41, 174
BstV1I GCAGC 5 cut(s) 169, 555, 600, 905, 920
BstV2I GAAGAC 1 cut(s) 567
BsuRI GGCC 5 cut(s) 18, 59, 204, 222, 387
BtgI CCRYGG 2 cut(s) 13, 226
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 6 cut(s) 91, 681, 730, 971, 1003, 1088
BtsIMutI CAGTG 2 cut(s) 700, 814
Cac8I GCNNGC 1 cut(s) 1013
CfoI GCGC 2 cut(s) 41, 176
Cfr10I RCCGGY 2 cut(s) 537, 917
Cfr13I GGNCC 5 cut(s) 230, 266, 482, 717, 1037
Csp6I GTAC 1 cut(s) 755
CspAI ACCGGT 1 cut(s) 537
CviAII CATG 8 cut(s) 130, 155, 227, 443, 629, 665, 1026, 1061
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 3 cut(s) 208, 278, 519
DpnI GATC 5 cut(s) 90, 449, 970, 1053, 1110
DpnII GATC 5 cut(s) 88, 447, 968, 1051, 1108
DraI TTTAAA 2 cut(s) 73, 874
DriI GACNNNNNGTC 1 cut(s) 1043
EaeI YGGCCR 4 cut(s) 16, 57, 202, 385
Eam1105I GACNNNNNGTC 1 cut(s) 1043
Eco130I CCWWGG 1 cut(s) 226
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 5 cut(s) 230, 266, 482, 717, 1037
Eco57I CTGAAG 2 cut(s) 213, 449
Eco88I CYCGRG 1 cut(s) 735
EcoO109I RGGNCCY 2 cut(s) 717, 1037
EcoRII CCWGG 1 cut(s) 268
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 683
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 8 cut(s) 133, 158, 230, 446, 632, 668, 1029, 1064
FalI AAGNNNNNCTT 4 cut(s) 291, 323, 552, 584
FaqI GGGAC 2 cut(s) 730, 953
FatI CATG 8 cut(s) 129, 154, 226, 442, 628, 664, 1025, 1060
FauNDI CATATG 2 cut(s) 452, 527
FbaI TGATCA 2 cut(s) 447, 1108
Fnu4HI GCNGC 8 cut(s) 183, 205, 220, 544, 589, 728, 894, 909
FokI GGATG 6 cut(s) 78, 668, 717, 958, 990, 1095
Fsp4HI GCNGC 8 cut(s) 183, 205, 220, 544, 589, 728, 894, 909
FspBI CTAG 2 cut(s) 254, 1008
GlaI GCGC 2 cut(s) 40, 175
GluI GCNGC 8 cut(s) 183, 205, 220, 544, 589, 728, 894, 909
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 5 cut(s) 18, 59, 204, 222, 387
HapII CCGG 4 cut(s) 388, 538, 918, 1080
HhaI GCGC 2 cut(s) 41, 176
Hin1II CATG 8 cut(s) 133, 158, 230, 446, 632, 668, 1029, 1064
Hin6I GCGC 2 cut(s) 39, 174
HinP1I GCGC 2 cut(s) 39, 174
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 3 cut(s) 161, 744, 815
HpaII CCGG 4 cut(s) 388, 538, 918, 1080
HphI GGTGA 4 cut(s) 238, 571, 689, 998
Hpy166II GTNNAC 2 cut(s) 160, 408
Hpy188I TCNGA 8 cut(s) 88, 396, 504, 551, 625, 686, 934, 1056
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 160, 408
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 7 cut(s) 179, 188, 409, 424, 464, 883, 929
HpyCH4III ACNGT 3 cut(s) 32, 704, 1033
HpyCH4IV ACGT 2 cut(s) 243, 998
HpyCH4V TGCA 8 cut(s) 456, 543, 588, 681, 837, 996, 1077, 1124
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 188, 204, 803, 1112, 1121
HpyF3I CTNAG 3 cut(s) 208, 278, 519
HpySE526I ACGT 2 cut(s) 243, 998
Hsp92II CATG 8 cut(s) 133, 158, 230, 446, 632, 668, 1029, 1064
HspAI GCGC 2 cut(s) 39, 174
KflI GGGWCCC 1 cut(s) 717
Ksp22I TGATCA 2 cut(s) 447, 1108
Kzo9I GATC 5 cut(s) 88, 447, 968, 1051, 1108
LmnI GCTCC 2 cut(s) 625, 650
Lsp1109I GCAGC 5 cut(s) 169, 555, 600, 905, 920
LweI GCATC 4 cut(s) 575, 668, 690, 739
MaeI CTAG 2 cut(s) 254, 1008
MaeII ACGT 2 cut(s) 243, 998
MaeIII GTNAC 5 cut(s) 32, 52, 244, 273, 688
MalI GATC 5 cut(s) 90, 449, 970, 1053, 1110
MboI GATC 5 cut(s) 88, 447, 968, 1051, 1108
MboII GAAGA 3 cut(s) 572, 871, 1001
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 6 cut(s) 378, 457, 603, 645, 693, 769
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 912, 1093
Mph1103I ATGCAT 1 cut(s) 683
MseI TTAA 4 cut(s) 72, 402, 873, 927
MslI CAYNNNNRTG 2 cut(s) 134, 669
MspA1I CMGCKG 3 cut(s) 727, 896, 1106
MspI CCGG 4 cut(s) 388, 538, 918, 1080
MspR9I CCNGG 2 cut(s) 270, 389
MunI CAATTG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 2 cut(s) 41, 174
MwoI GCNNNNNNNGC 6 cut(s) 182, 188, 204, 803, 1112, 1121
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 226
NdeI CATATG 2 cut(s) 452, 527
NdeII GATC 5 cut(s) 88, 447, 968, 1051, 1108
NlaIII CATG 8 cut(s) 133, 158, 230, 446, 632, 668, 1029, 1064
NlaIV GGNNCC 2 cut(s) 718, 719
NmuCI GTSAC 2 cut(s) 244, 273
NsiI ATGCAT 1 cut(s) 683
NspI RCATGY 2 cut(s) 446, 1064
PaeR7I CTCGAG 1 cut(s) 735
PciI ACATGT 1 cut(s) 1060
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 2 cut(s) 744, 815
PinAI ACCGGT 1 cut(s) 537
PkrI GCNGC 8 cut(s) 184, 206, 221, 545, 590, 729, 895, 910
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PpuMI RGGWCCY 2 cut(s) 717, 1037
PscI ACATGT 1 cut(s) 1060
Psp5II RGGWCCY 2 cut(s) 717, 1037
Psp6I CCWGG 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 718, 719
PspPI GGNCC 5 cut(s) 230, 266, 482, 717, 1037
PspPPI RGGWCCY 2 cut(s) 717, 1037
PspXI VCTCGAGB 1 cut(s) 735
PvuII CAGCTG 1 cut(s) 1106
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
RseI CAYNNNNRTG 2 cut(s) 134, 669
SaqAI TTAA 4 cut(s) 72, 402, 873, 927
SatI GCNGC 8 cut(s) 183, 205, 220, 544, 589, 728, 894, 909
Sau3AI GATC 5 cut(s) 88, 447, 968, 1051, 1108
Sau96I GGNCC 5 cut(s) 230, 266, 482, 717, 1037
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 270, 389
SduI GDGCHC 1 cut(s) 335
SfaNI GCATC 4 cut(s) 575, 668, 690, 739
Sfr274I CTCGAG 1 cut(s) 735
SinI GGWCC 5 cut(s) 230, 266, 482, 717, 1037
SlaI CTCGAG 1 cut(s) 735
SmiMI CAYNNNNRTG 2 cut(s) 134, 669
SmlI CTYRAG 2 cut(s) 115, 735
SmoI CTYRAG 2 cut(s) 115, 735
Sse9I AATT 6 cut(s) 378, 457, 603, 645, 693, 769
SsiI CCGC 5 cut(s) 22, 205, 219, 727, 896
SspMI CTAG 2 cut(s) 254, 1008
StyD4I CCNGG 2 cut(s) 268, 387
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 3 cut(s) 32, 704, 1033
TaiI ACGT 2 cut(s) 246, 1001
TaqI TCGA 5 cut(s) 292, 736, 778, 1047, 1099
TasI AATT 6 cut(s) 378, 457, 603, 645, 693, 769
TatI WGTACW 1 cut(s) 754
TauI GCSGC 3 cut(s) 207, 222, 730
TfiI GAWTC 2 cut(s) 744, 815
Tru1I TTAA 4 cut(s) 72, 402, 873, 927
Tru9I TTAA 4 cut(s) 72, 402, 873, 927
TscAI CASTG 2 cut(s) 707, 814
TseFI GTSAC 2 cut(s) 244, 273
TseI GCWGC 5 cut(s) 182, 543, 588, 893, 908
Tsp45I GTSAC 2 cut(s) 244, 273
TspDTI ATGAA 5 cut(s) 573, 861, 913, 937, 992
TspGWI ACGGA 2 cut(s) 579, 608
TspRI CASTG 2 cut(s) 707, 814
VpaK11BI GGWCC 5 cut(s) 230, 266, 482, 717, 1037
XceI RCATGY 2 cut(s) 446, 1064
XhoI CTCGAG 1 cut(s) 735
XspI CTAG 2 cut(s) 254, 1008
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.