Rorug03G0090000

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009024
Physical Location & Seq
Reverse (-)
7001511 .. 7006299
4789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0090000.1

Sequence Viewer

Length: 1134 bp
ATGGTGAAATTTAACAATGGCAAGCATAAGCAAACATGGGTATTGTTTAATTTGCTAAAGTTTGTCTTTTTGGGTTTTTACTTGACAGAATCATATACTCTTGACACAAACTGGAAATCAACAATGGCAAGCACAAGCTCCAAACCCAAAATCAAGACTAGGATCAAGATTAACAGACCCCTTTATATCAACTTACTGTTTGACAAGGAAAAACTTCCTTCATCTCTTTGGGGAGGCGGGCCCACCATAGTCCCTGGACCAGGAGGACCGGAGGACTTGCCGGAAGTTTTCAACAAGGTGATGAACACCACCGAAGGATTCATGAAGAGTTTTGTCAGGATGCATGGTAATCTGTTAAAAGACGGCTATGAGGACTTGGCTCATGAGATTGAAACTCAGTTAACGGAAAACGACATTGTTCCCATGATTATTGTGGTCACCGGCATCATTGAGGCGTACGCGGATGCTGGCAAGACCATGGAAGCTCTGGTGTTGTACCTGATTCTTTTGGTCTCTAATTGTGTCCCCAACCCTTACACATATAGTGTTCTCATCAAGGCACTGGCTGCAGACCCGGACCCCAAATTTCTTGAGGTTGCAAAGGAGTGCAAACTGGAGATGATGGAAAAGGGATGGCAGCCCAATGCCGAGACCTACACTGCCGTGTTTAAGGCATTTGCTTTGCAGGAGAAGGTCGAAGAGGGTAAGGAGTTTCGGGAGGAAATGGAAGCTAAGGGCTTCGTGGCAGACAAGATGGCTGTGATTGATGCTCTCAAGGAGACTACAGGACCGGTGGTTAAAAGCATTCACAACATTCTCTTTGATGACTCAGAGTACTCCGACTCAGATGACGATGATGATATAAAGAAATTCTACAAACTGATTGACTTGCAGAAGCTTTCTCCTTATAGTTTTGTAACTCCTCTCCTCAAGATTCCTGATATTGAAAATGACCCAGAGAACCCCAAAGATTTTCAACAAGTCTTCAATAAGATGCACACCGCGGAAGGTTTTTTCAAAACTGTGGTAAGGATGTTCAAAATGCTAGAACGTGAAGGCTTCCTATCTCAGGCTCAAGAGATCAGGGCCAAGATATATTCTTTTTGTGAATCTAATGATATGCCGAAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

377

Amino Acids

42.95

Weight (kDa)

5.18

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 144 - 189 2.5e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 461, 1004
AciI CCGC 4 cut(s) 237, 461, 1002, 1004
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 3 cut(s) 8, 584, 869
AfaI GTAC 3 cut(s) 458, 497, 836
AfiI CCNNNNNNNGG 2 cut(s) 260, 1069
AgeI ACCGGT 1 cut(s) 790
AgsI TTSAA 7 cut(s) 292, 392, 947, 977, 988, 1018, 1039
AjnI CCWGG 2 cut(s) 253, 259
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 4 cut(s) 138, 485, 731, 898
AluI AGCT 4 cut(s) 138, 485, 731, 898
Alw26I GTCTC 3 cut(s) 517, 644, 773
AlwI GGATC 1 cut(s) 170
AoxI GGCC 2 cut(s) 239, 1086
ApaI GGGCCC 1 cut(s) 243
ApeKI GCWGC 2 cut(s) 566, 637
ApoI RAATTY 3 cut(s) 8, 584, 869
AsiGI ACCGGT 1 cut(s) 790
Asp700I GAANNNNTTC 1 cut(s) 213
AspS9I GGNCC 7 cut(s) 239, 240, 257, 266, 577, 788, 1086
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 3 cut(s) 16, 310, 430
AvaII GGWCC 4 cut(s) 257, 266, 577, 788
BaeGI GKGCMC 1 cut(s) 243
BanII GRGCYC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 976
BbvI GCAGC 2 cut(s) 553, 649
BccI CCATC 3 cut(s) 616, 627, 748
BceAI ACGGC 2 cut(s) 379, 647
BciT130I CCWGG 2 cut(s) 255, 261
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 3 cut(s) 517, 644, 773
BfaI CTAG 2 cut(s) 159, 1046
BfmI CTRYAG 2 cut(s) 567, 783
BisI GCNGC 2 cut(s) 567, 638
BlsI GCNGC 2 cut(s) 568, 639
BmcAI AGTACT 1 cut(s) 836
Bme1390I CCNGG 3 cut(s) 255, 261, 575
Bme18I GGWCC 4 cut(s) 257, 266, 577, 788
BmgT120I GGNCC 7 cut(s) 239, 240, 257, 266, 577, 788, 1086
BmiI GGNNCC 2 cut(s) 241, 579
BmrFI CCNGG 3 cut(s) 255, 261, 575
BmsI GCATC 5 cut(s) 330, 453, 454, 757, 984
BpiI GAAGAC 1 cut(s) 976
BpmI CTGGAG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 4 cut(s) 611, 758, 914, 1059
BpuMI CCSGG 1 cut(s) 575
BsaI GGTCTC 2 cut(s) 517, 644
BsaJI CCNNGG 3 cut(s) 253, 477, 1002
BsaWI WCCGGW 2 cut(s) 268, 790
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 1069
Bse118I RCCGGY 2 cut(s) 440, 790
Bse1I ACTGG 3 cut(s) 116, 567, 618
BseBI CCWGG 2 cut(s) 255, 261
BseDI CCNNGG 3 cut(s) 253, 477, 1002
BseGI GGATG 4 cut(s) 345, 469, 638, 1038
BseLI CCNNNNNNNGG 2 cut(s) 260, 1069
BseMII CTCAG 4 cut(s) 410, 843, 858, 1082
BseNI ACTGG 3 cut(s) 116, 567, 618
BseRI GAGGAG 2 cut(s) 912, 917
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 2 cut(s) 553, 649
Bsh1236I CGCG 2 cut(s) 461, 1004
BshFI GGCC 2 cut(s) 241, 1088
BshTI ACCGGT 1 cut(s) 790
BsiSI CCGG 5 cut(s) 269, 281, 441, 575, 791
BsiWI CGTACG 1 cut(s) 456
BslFI GGGAC 2 cut(s) 236, 509
BslI CCNNNNNNNGG 2 cut(s) 260, 1069
BsmAI GTCTC 3 cut(s) 517, 644, 773
BsmFI GGGAC 2 cut(s) 236, 509
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 2 cut(s) 241, 1088
Bso31I GGTCTC 2 cut(s) 517, 644
Bsp120I GGGCCC 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 162, 1080
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 4 cut(s) 237, 461, 1002, 1004
BspANI GGCC 2 cut(s) 241, 1088
BspCNI CTCAG 4 cut(s) 409, 842, 857, 1081
BspFNI CGCG 2 cut(s) 461, 1004
BspHI TCATGA 2 cut(s) 321, 382
BspLI GGNNCC 2 cut(s) 241, 579
BspMAI CTGCAG 1 cut(s) 571
BspPI GGATC 1 cut(s) 170
BspTNI GGTCTC 2 cut(s) 517, 644
BsrFI RCCGGY 2 cut(s) 440, 790
BsrI ACTGG 3 cut(s) 116, 567, 618
BssAI RCCGGY 2 cut(s) 440, 790
BssECI CCNNGG 3 cut(s) 253, 477, 1002
BssMI GATC 2 cut(s) 162, 1080
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 255, 261
Bst4CI ACNGT 2 cut(s) 198, 1024
Bst6I CTCTTC 2 cut(s) 320, 693
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 4 cut(s) 23, 130, 239, 469
BstDEI CTNAG 5 cut(s) 396, 732, 829, 844, 1068
BstDSI CCRYGG 2 cut(s) 477, 1002
BstEII GGTNACC 1 cut(s) 436
BstENI CCTNNNNNAGG 2 cut(s) 258, 1067
BstF5I GGATG 4 cut(s) 345, 469, 638, 1038
BstFNI CGCG 2 cut(s) 461, 1004
BstKTI GATC 2 cut(s) 165, 1083
BstMAI GTCTC 3 cut(s) 517, 644, 773
BstMBI GATC 2 cut(s) 162, 1080
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNI CCWGG 2 cut(s) 255, 261
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 3 cut(s) 253, 259, 573
BstSFI CTRYAG 2 cut(s) 567, 783
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 2 cut(s) 461, 1004
BstV1I GCAGC 2 cut(s) 553, 649
BstV2I GAAGAC 1 cut(s) 976
BsuRI GGCC 2 cut(s) 241, 1088
BtgI CCRYGG 2 cut(s) 477, 1002
BtsCI GGATG 4 cut(s) 345, 469, 638, 1038
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 2 cut(s) 560, 657
Cac8I GCNNGC 4 cut(s) 23, 130, 239, 469
CciI TCATGA 2 cut(s) 321, 382
Cfr10I RCCGGY 2 cut(s) 440, 790
Cfr13I GGNCC 7 cut(s) 239, 240, 257, 266, 577, 788, 1086
Cfr42I CCGCGG 1 cut(s) 1005
Csp6I GTAC 3 cut(s) 457, 496, 835
CspAI ACCGGT 1 cut(s) 790
CviAII CATG 6 cut(s) 36, 322, 344, 383, 424, 478
CviQI GTAC 3 cut(s) 457, 496, 835
DdeI CTNAG 5 cut(s) 396, 732, 829, 844, 1068
DpnI GATC 2 cut(s) 164, 1082
DpnII GATC 2 cut(s) 162, 1080
Eam1104I CTCTTC 2 cut(s) 320, 693
EarI CTCTTC 2 cut(s) 320, 693
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 2 cut(s) 517, 644
Eco47I GGWCC 4 cut(s) 257, 266, 577, 788
Eco91I GGTNACC 1 cut(s) 436
EcoNI CCTNNNNNAGG 2 cut(s) 258, 1067
EcoO65I GGTNACC 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 253, 259
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 6 cut(s) 39, 325, 347, 386, 427, 481
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 2 cut(s) 236, 509
FatI CATG 6 cut(s) 35, 321, 343, 382, 423, 477
FauI CCCGC 1 cut(s) 230
Fnu4HI GCNGC 2 cut(s) 567, 638
FokI GGATG 4 cut(s) 352, 476, 645, 1045
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 2 cut(s) 567, 638
FspBI CTAG 2 cut(s) 159, 1046
GluI GCNGC 2 cut(s) 567, 638
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 2 cut(s) 241, 1088
HapII CCGG 5 cut(s) 269, 281, 441, 575, 791
Hin1II CATG 6 cut(s) 39, 325, 347, 386, 427, 481
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 7 cut(s) 89, 318, 502, 827, 842, 934, 1109
HpaI GTTAAC 1 cut(s) 402
HpaII CCGG 5 cut(s) 269, 281, 441, 575, 791
HphI GGTGA 3 cut(s) 16, 310, 430
Hpy166II GTNNAC 1 cut(s) 402
Hpy188I TCNGA 3 cut(s) 832, 841, 847
Hpy8I GTNNAC 1 cut(s) 402
HpyAV CCTTC 5 cut(s) 228, 308, 685, 1001, 1049
HpyCH4III ACNGT 2 cut(s) 198, 1024
HpyCH4IV ACGT 1 cut(s) 1051
HpyCH4V TGCA 7 cut(s) 343, 569, 599, 609, 685, 892, 997
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 5 cut(s) 396, 732, 829, 844, 1068
HpySE526I ACGT 1 cut(s) 1051
Hsp92II CATG 6 cut(s) 39, 325, 347, 386, 427, 481
KspAI GTTAAC 1 cut(s) 402
KspI CCGCGG 1 cut(s) 1005
Kzo9I GATC 2 cut(s) 162, 1080
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 2 cut(s) 553, 649
LweI GCATC 5 cut(s) 330, 453, 454, 757, 984
MaeI CTAG 2 cut(s) 159, 1046
MaeII ACGT 1 cut(s) 1051
MaeIII GTNAC 2 cut(s) 436, 916
MalI GATC 2 cut(s) 164, 1082
MboI GATC 2 cut(s) 162, 1080
MboII GAAGA 3 cut(s) 337, 710, 976
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 5 cut(s) 8, 49, 517, 584, 869
MlyI GAGTC 2 cut(s) 821, 836
MmeI TCCRAC 1 cut(s) 864
Mph1103I ATGCAT 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 7 cut(s) 12, 48, 171, 356, 401, 669, 798
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 1004
MspI CCGG 5 cut(s) 269, 281, 441, 575, 791
MspR9I CCNGG 3 cut(s) 255, 261, 575
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 2 cut(s) 255, 261
MvnI CGCG 2 cut(s) 461, 1004
MwoI GCNNNNNNNGC 1 cut(s) 566
NciI CCSGG 1 cut(s) 575
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 162, 1080
NlaIII CATG 6 cut(s) 39, 325, 347, 386, 427, 481
NlaIV GGNNCC 2 cut(s) 241, 579
NmeAIII GCCGAG 1 cut(s) 673
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 345
OliI CACNNNNGTG 1 cut(s) 662
PagI TCATGA 2 cut(s) 321, 382
PctI GAATGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 5 cut(s) 89, 318, 502, 934, 1109
Pfl23II CGTACG 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 790
PkrI GCNGC 2 cut(s) 568, 639
PleI GAGTC 2 cut(s) 821, 836
PpsI GAGTC 2 cut(s) 821, 836
Psp6I CCWGG 2 cut(s) 253, 259
PspEI GGTNACC 1 cut(s) 436
PspGI CCWGG 2 cut(s) 253, 259
PspLI CGTACG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 241, 579
PspOMI GGGCCC 1 cut(s) 239
PspPI GGNCC 7 cut(s) 239, 240, 257, 266, 577, 788, 1086
PstI CTGCAG 1 cut(s) 571
RsaI GTAC 3 cut(s) 458, 497, 836
RsaNI GTAC 3 cut(s) 457, 496, 835
RseI CAYNNNNRTG 1 cut(s) 662
SacII CCGCGG 1 cut(s) 1005
SaqAI TTAA 7 cut(s) 12, 48, 171, 356, 401, 669, 798
SatI GCNGC 2 cut(s) 567, 638
Sau3AI GATC 2 cut(s) 162, 1080
Sau96I GGNCC 7 cut(s) 239, 240, 257, 266, 577, 788, 1086
ScaI AGTACT 1 cut(s) 836
SchI GAGTC 2 cut(s) 821, 836
ScrFI CCNGG 3 cut(s) 255, 261, 575
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 5 cut(s) 330, 453, 454, 757, 984
SfcI CTRYAG 2 cut(s) 567, 783
Sfr303I CCGCGG 1 cut(s) 1005
SgrBI CCGCGG 1 cut(s) 1005
SinI GGWCC 4 cut(s) 257, 266, 577, 788
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 4 cut(s) 590, 773, 929, 1074
SmoI CTYRAG 4 cut(s) 590, 773, 929, 1074
Sse9I AATT 5 cut(s) 8, 49, 517, 584, 869
SsiI CCGC 4 cut(s) 237, 461, 1002, 1004
SspMI CTAG 2 cut(s) 159, 1046
StyD4I CCNGG 3 cut(s) 253, 259, 573
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 2 cut(s) 198, 1024
TaiI ACGT 1 cut(s) 1054
TaqI TCGA 1 cut(s) 696
TasI AATT 5 cut(s) 8, 49, 517, 584, 869
TatI WGTACW 1 cut(s) 834
TfiI GAWTC 5 cut(s) 89, 318, 502, 934, 1109
Tru1I TTAA 7 cut(s) 12, 48, 171, 356, 401, 669, 798
Tru9I TTAA 7 cut(s) 12, 48, 171, 356, 401, 669, 798
TscAI CASTG 2 cut(s) 567, 664
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 566, 637
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 210, 310, 317, 338
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 567, 664
VpaK11BI GGWCC 4 cut(s) 257, 266, 577, 788
XagI CCTNNNNNAGG 2 cut(s) 258, 1067
XapI RAATTY 3 cut(s) 8, 584, 869
XcmI CCANNNNNNNNNTGG 2 cut(s) 430, 484
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 2 cut(s) 159, 1046
ZrmI AGTACT 1 cut(s) 836
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.