MD12G1133200.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009024
Physical Location & Seq
Forward (+)
20864790 .. 20867800
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1133200.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGGCAACTCCCACGGCCACCACCGTAAACGCCGTAATGGCCCCGGGTCCAGTGAAACGGCCAATTCGGGCTTTAAATATGGTCCGTCGGATCAAGCGGCTCAAATCCCTAGCTCTAGCTCAACGTCAGCTAAGAAGACCAGCAGCAGCATGGTGGTCGCCTTGCCGTACGCTCACGTCGACTCGAGCTTGCGCGCCCTCTCCGCCCAGGCCGAGGGCTTCGGCCGGCTCGCAATCGGCGGCCTCCACGGCGCCGTTTACCACGTCACCACTCTAGCAGATGATGGTCCGGGTTCACTTAGGGAAGGATGTCGTCAGAAAGAACCGCTTTGGATTGTCTTCGAAATTTCAGGCACTATCCATCTCCAATCTTACTTGAACGTGTCATCGTATAAGACTATAGATGGGCGTGGTCAGAGGATTAAACTTACAGGGAAGGGGTTGAGGTTGAAGGAATGTGAACATGTCATTGTGTGCAATTTAGAGTTTGAAGGTGGTAGAGGGCCAGATGTTGATGGCATTCAAATAAAACCCAATTCTAAACACATATGGATAGATCGCTGCAGCCTTCGTGATTATGAAGACGGACTTATCGATATCACCAGAGGAAGCACTGATATTACAATTTCCAGGTGTCATTTTTCACAGCATGATAAGACAATGCTGATTGGCTCAGATCCCTCTCATATTGATGACAGATGCATCCGGGTTACCATTCACCACTGTTTCTTTGATGGGACCAGACAGAGGCATCCTCGTGTTAGATTTGCAAAAGTACATCTATATAACAATTACACCCGAAACTGGGGCATATATGCTGTTTGTGCTAGTGTAGAATCGCAGATATTCTCGGAATGCAATATATATGAAGCAGGACAGAAAAAAGTGGCATTTAAGTACCTTACGGAGAAGGCAGCGGACAAGGAGACGCCAATGACTGGCCACATTAAGTCCGAAGGCGACTTATTTGTAACGGGAACTCAAGCAGGATTATCCCCCGATGGCGGTGAACATTGCGTGTTTCATCCTACCCAGCACTATCAGACATGGACAAAAGGACCGCCCGTGGACGATCTTAAGCATGTTCTCCAACATTGCGCAGGATGGCAGTGTGTTCCGCGGCCAGCTGATCAAGGATTAGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

383

Amino Acids

42.19

Weight (kDa)

8.52

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 124 - 309 1.6e-29 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1099
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 938
AccI GTMKAC 1 cut(s) 180
AccII CGCG 2 cut(s) 195, 1120
AciI CCGC 9 cut(s) 98, 204, 240, 326, 917, 1005, 1061, 1118, 1120
AclWI GGATC 2 cut(s) 99, 671
AcoI YGGCCR 5 cut(s) 16, 60, 223, 940, 1121
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 2 cut(s) 252, 929
AfaI GTAC 3 cut(s) 170, 777, 899
AfiI CCNNNNNNNGG 6 cut(s) 214, 747, 804, 805, 938, 1004
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 2 cut(s) 381, 463
AgsI TTSAA 4 cut(s) 379, 451, 491, 524
AjiI CACGTC 2 cut(s) 178, 265
AjnI CCWGG 2 cut(s) 207, 629
AluBI AGCT 5 cut(s) 114, 120, 131, 189, 1127
AluI AGCT 5 cut(s) 114, 120, 131, 189, 1127
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 2 cut(s) 99, 671
Ama87I CYCGRG 2 cut(s) 44, 184
AoxI GGCC 9 cut(s) 16, 40, 60, 210, 223, 241, 503, 940, 1121
ApeKI GCWGC 5 cut(s) 144, 147, 561, 564, 914
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 4 cut(s) 195, 197, 254, 1100
AspS9I GGNCC 7 cut(s) 41, 48, 83, 287, 503, 738, 1058
AsuC2I CCSGG 4 cut(s) 45, 46, 291, 707
AsuHPI GGTGA 4 cut(s) 259, 592, 710, 1019
AsuII TTCGAA 1 cut(s) 342
AvaI CYCGRG 2 cut(s) 44, 184
AvaII GGWCC 5 cut(s) 48, 83, 287, 738, 1058
BalI TGGCCA 1 cut(s) 942
BanI GGYRCC 1 cut(s) 251
BauI CACGAG 1 cut(s) 756
BbsI GAAGAC 3 cut(s) 143, 331, 588
BbvI GCAGC 5 cut(s) 156, 159, 548, 576, 926
BccI CCATC 7 cut(s) 278, 369, 398, 509, 728, 995, 1098
BceAI ACGGC 6 cut(s) 18, 31, 75, 151, 239, 265
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 2 cut(s) 209, 631
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 4 cut(s) 45, 46, 291, 707
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 4 cut(s) 111, 117, 275, 828
BfmI CTRYAG 2 cut(s) 399, 562
BfoI RGCGCY 1 cut(s) 255
BfrI CTTAAG 1 cut(s) 1076
BglI GCCNNNNNGGC 2 cut(s) 39, 249
BisI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
BlsI GCNGC 8 cut(s) 100, 146, 149, 242, 563, 566, 916, 1122
Bme1390I CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
Bme18I GGWCC 5 cut(s) 48, 83, 287, 738, 1058
BmeT110I CYCGRG 2 cut(s) 44, 184
BmgBI CACGTC 2 cut(s) 178, 265
BmgT120I GGNCC 7 cut(s) 41, 48, 83, 287, 503, 738, 1058
BmiI GGNNCC 4 cut(s) 43, 49, 253, 739
BmrFI CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 3 cut(s) 689, 711, 760
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 3 cut(s) 143, 331, 588
BplI GAGNNNNNCTC 2 cut(s) 739, 771
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 966
BpuMI CCSGG 4 cut(s) 45, 46, 291, 707
Bsa29I ATCGAT 1 cut(s) 594
BsaHI GRCGYC 2 cut(s) 252, 929
BsaJI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 747, 804, 805, 938, 1004
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 3 cut(s) 51, 809, 943
Bse3DI GCAATG 2 cut(s) 1012, 1093
BseBI CCWGG 2 cut(s) 209, 631
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
BseGI GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BseLI CCNNNNNNNGG 6 cut(s) 214, 747, 804, 805, 938, 1004
BseMI GCAATG 2 cut(s) 1012, 1093
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 3 cut(s) 51, 809, 943
BsePI GCGCGC 1 cut(s) 193
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 5 cut(s) 156, 159, 548, 576, 926
BseYI CCCAGC 1 cut(s) 1032
Bsh1236I CGCG 2 cut(s) 195, 1120
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 9 cut(s) 18, 42, 62, 212, 225, 243, 505, 942, 1123
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 2 cut(s) 44, 184
BsiSI CCGG 4 cut(s) 45, 226, 290, 706
BsiWI CGTACG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 6 cut(s) 214, 747, 804, 805, 938, 1004
BsmAI GTCTC 1 cut(s) 920
BsmBI CGTCTC 1 cut(s) 920
BsmFI GGGAC 1 cut(s) 751
BsmI GAATGC 2 cut(s) 519, 860
BsnI GGCC 9 cut(s) 18, 42, 62, 212, 225, 243, 505, 942, 1123
BsoBI CYCGRG 2 cut(s) 44, 184
Bsp119I TTCGAA 1 cut(s) 342
Bsp143I GATC 5 cut(s) 91, 556, 676, 1072, 1129
BspACI CCGC 9 cut(s) 98, 204, 240, 326, 917, 1005, 1061, 1118, 1120
BspANI GGCC 9 cut(s) 18, 42, 62, 212, 225, 243, 505, 942, 1123
BspCNI CTCAG 1 cut(s) 686
BspDI ATCGAT 1 cut(s) 594
BspFNI CGCG 2 cut(s) 195, 1120
BspLI GGNNCC 4 cut(s) 43, 49, 253, 739
BspMAI CTGCAG 1 cut(s) 566
BspPI GGATC 2 cut(s) 99, 671
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 251
BspTI CTTAAG 1 cut(s) 1076
BsrDI GCAATG 2 cut(s) 1012, 1093
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 3 cut(s) 51, 809, 943
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
BssHII GCGCGC 1 cut(s) 193
BssMI GATC 5 cut(s) 91, 556, 676, 1072, 1129
BssNI GRCGYC 2 cut(s) 252, 929
BssSI CACGAG 1 cut(s) 756
Bst2BI CACGAG 1 cut(s) 756
Bst2UI CCWGG 2 cut(s) 209, 631
Bst4CI ACNGT 2 cut(s) 26, 725
BstACI GRCGYC 2 cut(s) 252, 929
BstAFI CTTAAG 1 cut(s) 1076
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 5 cut(s) 191, 195, 227, 231, 1125
BstDEI CTNAG 3 cut(s) 132, 299, 673
BstDSI CCRYGG 4 cut(s) 13, 247, 1065, 1118
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BstFNI CGCG 2 cut(s) 195, 1120
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 4 cut(s) 195, 197, 254, 1100
BstKTI GATC 5 cut(s) 94, 559, 679, 1075, 1132
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 5 cut(s) 91, 556, 676, 1072, 1129
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
BstNI CCWGG 2 cut(s) 209, 631
BstNSI RCATGY 2 cut(s) 467, 1085
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 6 cut(s) 43, 44, 207, 289, 629, 705
BstSFI CTRYAG 2 cut(s) 399, 562
BstUI CGCG 2 cut(s) 195, 1120
BstV1I GCAGC 5 cut(s) 156, 159, 548, 576, 926
BstV2I GAAGAC 3 cut(s) 143, 331, 588
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZI CGGCCG 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 594
BsuRI GGCC 9 cut(s) 18, 42, 62, 212, 225, 243, 505, 942, 1123
BsuTUI ATCGAT 1 cut(s) 594
BtgI CCRYGG 4 cut(s) 13, 247, 1065, 1118
BtrI CACGTC 2 cut(s) 178, 265
BtsCI GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BtsI GCAGTG 1 cut(s) 1115
BtsIMutI CAGTG 4 cut(s) 58, 612, 721, 1115
Cac8I GCNNGC 5 cut(s) 191, 195, 227, 231, 1125
CfoI GCGC 4 cut(s) 195, 197, 254, 1100
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 7 cut(s) 41, 48, 83, 287, 503, 738, 1058
Cfr42I CCGCGG 1 cut(s) 1121
Cfr9I CCCGGG 1 cut(s) 44
ClaI ATCGAT 1 cut(s) 594
CseI GACGC 1 cut(s) 937
Csp6I GTAC 3 cut(s) 169, 776, 898
CviAII CATG 5 cut(s) 151, 464, 650, 1047, 1082
CviQI GTAC 3 cut(s) 169, 776, 898
DdeI CTNAG 3 cut(s) 132, 299, 673
DinI GGCGCC 1 cut(s) 253
DpnI GATC 5 cut(s) 93, 558, 678, 1074, 1131
DpnII GATC 5 cut(s) 91, 556, 676, 1072, 1129
DraI TTTAAA 1 cut(s) 76
EaeI YGGCCR 5 cut(s) 16, 60, 223, 940, 1121
EagI CGGCCG 1 cut(s) 223
EciI GGCGGA 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 223
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 5 cut(s) 48, 83, 287, 738, 1058
Eco52I CGGCCG 1 cut(s) 223
Eco88I CYCGRG 2 cut(s) 44, 184
Eco91I GGTNACC 1 cut(s) 709
EcoO65I GGTNACC 1 cut(s) 709
EcoRII CCWGG 2 cut(s) 207, 629
EcoRV GATATC 1 cut(s) 598
EcoT22I ATGCAT 1 cut(s) 704
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 5 cut(s) 154, 467, 653, 1050, 1085
FalI AAGNNNNNCTT 4 cut(s) 312, 344, 573, 605
FaqI GGGAC 1 cut(s) 751
FatI CATG 5 cut(s) 150, 463, 649, 1046, 1081
FauNDI CATATG 1 cut(s) 548
FbaI TGATCA 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
FokI GGATG 5 cut(s) 321, 689, 738, 1011, 1116
Fsp4HI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
FspBI CTAG 4 cut(s) 111, 117, 275, 828
FspI TGCGCA 1 cut(s) 1099
GlaI GCGC 4 cut(s) 194, 196, 253, 1099
GluI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
GsaI CCCAGC 1 cut(s) 1036
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 9 cut(s) 18, 42, 62, 212, 225, 243, 505, 942, 1123
HapII CCGG 4 cut(s) 45, 226, 290, 706
HgaI GACGC 1 cut(s) 937
HhaI GCGC 4 cut(s) 195, 197, 254, 1100
Hin1I GRCGYC 2 cut(s) 252, 929
Hin1II CATG 5 cut(s) 154, 467, 653, 1050, 1085
Hin6I GCGC 4 cut(s) 193, 195, 252, 1098
HinP1I GCGC 4 cut(s) 193, 195, 252, 1098
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 2 cut(s) 182, 836
HpaII CCGG 4 cut(s) 45, 226, 290, 706
HphI GGTGA 4 cut(s) 259, 592, 710, 1019
Hpy166II GTNNAC 7 cut(s) 29, 181, 259, 296, 461, 1010, 1069
Hpy188I TCNGA 7 cut(s) 91, 318, 417, 676, 853, 955, 1044
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 7 cut(s) 29, 181, 259, 296, 461, 1010, 1069
Hpy99I CGWCG 2 cut(s) 91, 182
HpyAV CCTTC 7 cut(s) 299, 430, 445, 485, 578, 904, 950
HpyCH4III ACNGT 2 cut(s) 26, 725
HpyCH4IV ACGT 4 cut(s) 125, 177, 264, 381
HpyCH4V TGCA 5 cut(s) 477, 564, 702, 770, 858
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
HpyF3I CTNAG 3 cut(s) 132, 299, 673
HpySE526I ACGT 4 cut(s) 125, 177, 264, 381
Hsp92I GRCGYC 2 cut(s) 252, 929
Hsp92II CATG 5 cut(s) 154, 467, 653, 1050, 1085
HspAI GCGC 4 cut(s) 193, 195, 252, 1098
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 1129
KspI CCGCGG 1 cut(s) 1121
Kzo9I GATC 5 cut(s) 91, 556, 676, 1072, 1129
Lsp1109I GCAGC 5 cut(s) 156, 159, 548, 576, 926
LweI GCATC 3 cut(s) 689, 711, 760
MaeI CTAG 4 cut(s) 111, 117, 275, 828
MaeII ACGT 4 cut(s) 125, 177, 264, 381
MaeIII GTNAC 3 cut(s) 265, 709, 970
MalI GATC 5 cut(s) 93, 558, 678, 1074, 1131
MboI GATC 5 cut(s) 91, 556, 676, 1072, 1129
MboII GAAGA 3 cut(s) 148, 331, 593
MflI RGATCY 1 cut(s) 676
MlsI TGGCCA 1 cut(s) 942
MluCI AATT 6 cut(s) 64, 345, 478, 535, 624, 790
MluNI TGGCCA 1 cut(s) 942
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 2 cut(s) 69, 1114
Mox20I TGGCCA 1 cut(s) 942
Mph1103I ATGCAT 1 cut(s) 704
MroNI GCCGGC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 942
MseI TTAA 5 cut(s) 75, 423, 894, 948, 1077
MslI CAYNNNNRTG 2 cut(s) 690, 756
Msp20I TGGCCA 1 cut(s) 942
MspA1I CMGCKG 3 cut(s) 917, 1120, 1127
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 4 cut(s) 45, 226, 290, 706
MspR9I CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
Mva1269I GAATGC 2 cut(s) 519, 860
MvaI CCWGG 2 cut(s) 209, 631
MvnI CGCG 2 cut(s) 195, 1120
MwoI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
NaeI GCCGGC 1 cut(s) 227
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 4 cut(s) 45, 46, 291, 707
NdeI CATATG 1 cut(s) 548
NdeII GATC 5 cut(s) 91, 556, 676, 1072, 1129
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 5 cut(s) 154, 467, 653, 1050, 1085
NlaIV GGNNCC 4 cut(s) 43, 49, 253, 739
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 265
NsbI TGCGCA 1 cut(s) 1099
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 2 cut(s) 467, 1085
NspV TTCGAA 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 184
PauI GCGCGC 1 cut(s) 193
PciI ACATGT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 591
PctI GAATGC 2 cut(s) 519, 860
PdiI GCCGGC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 836
Pfl23II CGTACG 1 cut(s) 168
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 8 cut(s) 100, 146, 149, 242, 563, 566, 916, 1122
PleI GAGTC 1 cut(s) 176
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 176
PscI ACATGT 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 207, 629
PspEI GGTNACC 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 1032
PspGI CCWGG 2 cut(s) 207, 629
PspLI CGTACG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 43, 49, 253, 739
PspPI GGNCC 7 cut(s) 41, 48, 83, 287, 503, 738, 1058
PspXI VCTCGAGB 1 cut(s) 184
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 676
PteI GCGCGC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 1127
RsaI GTAC 3 cut(s) 170, 777, 899
RsaNI GTAC 3 cut(s) 169, 776, 898
RseI CAYNNNNRTG 2 cut(s) 690, 756
SacII CCGCGG 1 cut(s) 1121
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 5 cut(s) 75, 423, 894, 948, 1077
SatI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
Sau3AI GATC 5 cut(s) 91, 556, 676, 1072, 1129
Sau96I GGNCC 7 cut(s) 41, 48, 83, 287, 503, 738, 1058
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
SfaNI GCATC 3 cut(s) 689, 711, 760
SfcI CTRYAG 2 cut(s) 399, 562
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 184
Sfr303I CCGCGG 1 cut(s) 1121
SfuI TTCGAA 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 1121
SinI GGWCC 5 cut(s) 48, 83, 287, 738, 1058
SlaI CTCGAG 1 cut(s) 184
SmaI CCCGGG 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 690, 756
SmlI CTYRAG 3 cut(s) 184, 981, 1076
SmoI CTYRAG 3 cut(s) 184, 981, 1076
Sse9I AATT 6 cut(s) 64, 345, 478, 535, 624, 790
SsiI CCGC 9 cut(s) 98, 204, 240, 326, 917, 1005, 1061, 1118, 1120
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 4 cut(s) 111, 117, 275, 828
StyD4I CCNGG 6 cut(s) 43, 44, 207, 289, 629, 705
TaaI ACNGT 2 cut(s) 26, 725
TaiI ACGT 4 cut(s) 128, 180, 267, 384
TaqI TCGA 4 cut(s) 180, 185, 342, 594
TasI AATT 6 cut(s) 64, 345, 478, 535, 624, 790
TatI WGTACW 1 cut(s) 775
TauI GCSGC 3 cut(s) 101, 243, 1123
TfiI GAWTC 1 cut(s) 836
Tru1I TTAA 5 cut(s) 75, 423, 894, 948, 1077
Tru9I TTAA 5 cut(s) 75, 423, 894, 948, 1077
TscAI CASTG 4 cut(s) 58, 619, 728, 1115
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 5 cut(s) 144, 147, 561, 564, 914
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 594, 882, 1013
TspGWI ACGGA 3 cut(s) 75, 600, 920
TspMI CCCGGG 1 cut(s) 44
TspRI CASTG 4 cut(s) 58, 619, 728, 1115
Van91I CCANNNNNTGG 1 cut(s) 938
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 5 cut(s) 48, 83, 287, 738, 1058
XapI RAATTY 1 cut(s) 345
XceI RCATGY 2 cut(s) 467, 1085
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 4 cut(s) 111, 117, 275, 828
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.