RLG00000024471

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009024
Physical Location & Seq
Forward (+)
36137627 .. 36140205
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024471

Sequence Viewer

Length: 1128 bp
ATGGGCAACTCCCACGGCCACCGCAAACACCGTAACGGCGCGAACCGCCCCGTAACGCCCAATCCACCCTTTAAATATGGCCCATCCGATCCCCAAGCTCATATCCCCAGCTCAGCTAAGCCCACCATGGCGGTGTCCTTGCCTTACTCTCATGTTGACTCCACCCTTCGCGCCCTCGCTGCCCAAGCTGAAGGCTTTGGTCGCTTAGCAATCGGCGGCCTCCATGGTCCTCTTTACCACGTTACTACTCTAGCCGATGATGGTCCAGGGTCACTTAGGGAAGGCTGTCGAAAGAAAGAGCCACTTTGGATTGTCTTTGATATTTCGGGCACTATCCATCTCTCGTCTTACTTGAATGTGTCGTCGTATAAGACAATTGATGGCCGGGGTCAGAGGATTAAGTTTACTGGGAAGGGGTTGAGGCTAAAGGAATGTGAGCATGTGATTATATGCAATCTAGAGTTTGAAGGTGGTCGAGGACCAGATGTTGATGGTATTCAGATAAAACCAAACTCTAAGCACATATGGATAGACCGGTGCAGCCTTCGTGATTATGAAGACGGACTTATAGATATCACCAGATGCAGCACGGATATTACAATTTCGAGGTGTCATTTCTCGCAGCATGATAAGACAATGCTAATCGGAGCAGACCCCTCTCATGTAGATGACAGATGCATCCGGGTTACAATTCACCACTGTTTCTTTGATGGGACCCGACAGCGGCATCCTCGTGTTAGATTCGCCAAAGTACATCTATACAACAATTACACCCGAAACTGGGGCATATATGCTGTTTGTGCCAGTGTAGAATCGCAGATATACTCTCAATGCAATATATATGAAGCAGGACAGAAGAAAATAGCGTTTAAATACCTTACAGAGAAGGCAGCGGACAGGGAGGCGGCAATGACTGGCTTCATTAGGTCGGAAGGGGACTTATTCATAACTGGAACTCAAGCAGGATTAGCCACCGAGGGTGGTGAAAATTGCATGTTTCATCCTAGCCAACACTACCAGACATGGACGGTAGGACCTCCAGTGGATGATCTGAAACATGTTCTCCAACATTGCACCGGATGGCAGTCCATTCCTCGGCCAGCTGATCAGGCAAGTATAGCACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

376

Amino Acids

41.77

Weight (kDa)

8.53

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 117 - 287 8.8e-28 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 41, 171
AciI CCGC 7 cut(s) 22, 46, 131, 216, 724, 893, 905
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 3 cut(s) 16, 382, 1097
AcuI CTGAAG 1 cut(s) 210
AfaI GTAC 1 cut(s) 753
AfiI CCNNNNNNNGG 4 cut(s) 723, 780, 781, 1095
AflIII ACRYGT 1 cut(s) 1057
AgeI ACCGGT 1 cut(s) 534
AgsI TTSAA 2 cut(s) 355, 467
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 5 cut(s) 98, 111, 116, 188, 1103
AluI AGCT 5 cut(s) 98, 111, 116, 188, 1103
AlwI GGATC 1 cut(s) 83
AoxI GGCC 5 cut(s) 16, 79, 217, 382, 1097
ApeKI GCWGC 5 cut(s) 179, 540, 585, 622, 890
AsiGI ACCGGT 1 cut(s) 534
AspLEI GCGC 2 cut(s) 41, 173
AspS9I GGNCC 6 cut(s) 80, 227, 263, 479, 714, 1034
AsuC2I CCSGG 2 cut(s) 386, 683
AsuHPI GGTGA 3 cut(s) 568, 686, 995
AvaII GGWCC 5 cut(s) 227, 263, 479, 714, 1034
BaeGI GKGCMC 1 cut(s) 332
BauI CACGAG 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 564
BbvI GCAGC 5 cut(s) 166, 552, 597, 634, 902
BccI CCATC 7 cut(s) 91, 254, 345, 374, 485, 704, 1074
BceAI ACGGC 2 cut(s) 31, 52
BciT130I CCWGG 1 cut(s) 267
BclI TGATCA 1 cut(s) 1105
BcnI CCSGG 2 cut(s) 386, 683
BfaI CTAG 3 cut(s) 251, 458, 1005
BisI GCNGC 8 cut(s) 180, 217, 541, 586, 623, 725, 891, 906
BlpI GCTNAGC 3 cut(s) 112, 117, 205
BlsI GCNGC 8 cut(s) 181, 218, 542, 587, 624, 726, 892, 907
Bme1390I CCNGG 3 cut(s) 267, 386, 683
Bme18I GGWCC 5 cut(s) 227, 263, 479, 714, 1034
BmgT120I GGNCC 6 cut(s) 80, 227, 263, 479, 714, 1034
BmiI GGNNCC 2 cut(s) 715, 716
BmrFI CCNGG 3 cut(s) 267, 386, 683
BmrI ACTGGG 2 cut(s) 417, 790
BmsI GCATC 4 cut(s) 572, 665, 687, 736
BmuI ACTGGG 2 cut(s) 417, 790
BpiI GAAGAC 1 cut(s) 564
BpmI CTGGAG 1 cut(s) 1023
Bpu1102I GCTNAGC 3 cut(s) 112, 117, 205
BpuEI CTTGAG 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 386, 683
BsaJI CCNNGG 7 cut(s) 13, 126, 223, 266, 385, 975, 1094
BsaWI WCCGGW 2 cut(s) 534, 1076
BsaXI ACNNNNNCTCC 2 cut(s) 1047, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 723, 780, 781, 1095
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 6 cut(s) 412, 785, 804, 919, 955, 1040
Bse3DI GCAATG 2 cut(s) 915, 1069
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 7 cut(s) 13, 126, 223, 266, 385, 975, 1094
BseGI GGATG 6 cut(s) 83, 678, 727, 1000, 1051, 1085
BseLI CCNNNNNNNGG 4 cut(s) 723, 780, 781, 1095
BseMI GCAATG 2 cut(s) 915, 1069
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 6 cut(s) 412, 785, 804, 919, 955, 1040
BseSI GKGCMC 1 cut(s) 332
BseXI GCAGC 5 cut(s) 166, 552, 597, 634, 902
BseYI CCCAGC 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 41, 171
BshFI GGCC 5 cut(s) 18, 81, 219, 384, 1099
BshTI ACCGGT 1 cut(s) 534
BsiSI CCGG 4 cut(s) 385, 535, 682, 1077
BslFI GGGAC 2 cut(s) 727, 950
BslI CCNNNNNNNGG 4 cut(s) 723, 780, 781, 1095
BsmFI GGGAC 2 cut(s) 727, 950
BsnI GGCC 5 cut(s) 18, 81, 219, 384, 1099
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 3 cut(s) 88, 1048, 1105
Bsp1720I GCTNAGC 3 cut(s) 112, 117, 205
Bsp19I CCATGG 2 cut(s) 126, 223
BspACI CCGC 7 cut(s) 22, 46, 131, 216, 724, 893, 905
BspANI GGCC 5 cut(s) 18, 81, 219, 384, 1099
BspCNI CTCAG 1 cut(s) 125
BspFNI CGCG 2 cut(s) 41, 171
BspLI GGNNCC 2 cut(s) 715, 716
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 915, 1069
BsrFI RCCGGY 1 cut(s) 534
BsrI ACTGG 6 cut(s) 412, 785, 804, 919, 955, 1040
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 7 cut(s) 13, 126, 223, 266, 385, 975, 1094
BssMI GATC 3 cut(s) 88, 1048, 1105
BssSI CACGAG 1 cut(s) 732
BssT1I CCWWGG 2 cut(s) 126, 223
Bst2BI CACGAG 1 cut(s) 732
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 4 cut(s) 32, 701, 1030, 1125
BstC8I GCNNGC 1 cut(s) 1101
BstDEI CTNAG 5 cut(s) 112, 117, 205, 275, 516
BstDSI CCRYGG 3 cut(s) 13, 126, 223
BstF5I GGATG 6 cut(s) 83, 678, 727, 1000, 1051, 1085
BstFNI CGCG 2 cut(s) 41, 171
BstHHI GCGC 2 cut(s) 41, 173
BstKTI GATC 3 cut(s) 91, 1051, 1108
BstMBI GATC 3 cut(s) 88, 1048, 1105
BstMWI GCNNNNNNNGC 8 cut(s) 45, 179, 185, 201, 800, 968, 1109, 1118
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 3 cut(s) 443, 997, 1061
BstSCI CCNGG 3 cut(s) 265, 384, 681
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 2 cut(s) 41, 171
BstV1I GCAGC 5 cut(s) 166, 552, 597, 634, 902
BstV2I GAAGAC 1 cut(s) 564
BsuRI GGCC 5 cut(s) 18, 81, 219, 384, 1099
BtgI CCRYGG 3 cut(s) 13, 126, 223
BtsCI GGATG 6 cut(s) 83, 678, 727, 1000, 1051, 1085
BtsIMutI CAGTG 3 cut(s) 697, 811, 1047
Cac8I GCNNGC 1 cut(s) 1101
CfoI GCGC 2 cut(s) 41, 173
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 6 cut(s) 80, 227, 263, 479, 714, 1034
Csp6I GTAC 1 cut(s) 752
CspAI ACCGGT 1 cut(s) 534
CviAII CATG 9 cut(s) 127, 152, 224, 440, 626, 662, 994, 1023, 1058
CviQI GTAC 1 cut(s) 752
DdeI CTNAG 5 cut(s) 112, 117, 205, 275, 516
DpnI GATC 3 cut(s) 90, 1050, 1107
DpnII GATC 3 cut(s) 88, 1048, 1105
DraI TTTAAA 2 cut(s) 73, 871
EaeI YGGCCR 3 cut(s) 16, 382, 1097
Eco130I CCWWGG 2 cut(s) 126, 223
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 5 cut(s) 227, 263, 479, 714, 1034
Eco57I CTGAAG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 714, 1034
EcoRII CCWGG 1 cut(s) 265
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 126, 223
EcoT22I ATGCAT 1 cut(s) 680
ErhI CCWWGG 2 cut(s) 126, 223
FaeI CATG 9 cut(s) 130, 155, 227, 443, 629, 665, 997, 1026, 1061
FalI AAGNNNNNCTT 4 cut(s) 288, 320, 549, 581
FaqI GGGAC 2 cut(s) 727, 950
FatI CATG 9 cut(s) 126, 151, 223, 439, 625, 661, 993, 1022, 1057
FauNDI CATATG 1 cut(s) 524
FbaI TGATCA 1 cut(s) 1105
Fnu4HI GCNGC 8 cut(s) 180, 217, 541, 586, 623, 725, 891, 906
FokI GGATG 6 cut(s) 70, 665, 714, 987, 1058, 1092
Fsp4HI GCNGC 8 cut(s) 180, 217, 541, 586, 623, 725, 891, 906
FspBI CTAG 3 cut(s) 251, 458, 1005
GlaI GCGC 2 cut(s) 40, 172
GluI GCNGC 8 cut(s) 180, 217, 541, 586, 623, 725, 891, 906
GsaI CCCAGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 5 cut(s) 18, 81, 219, 384, 1099
HapII CCGG 4 cut(s) 385, 535, 682, 1077
HhaI GCGC 2 cut(s) 41, 173
Hin1II CATG 9 cut(s) 130, 155, 227, 443, 629, 665, 997, 1026, 1061
Hin6I GCGC 2 cut(s) 39, 171
HinP1I GCGC 2 cut(s) 39, 171
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 3 cut(s) 158, 741, 812
HpaII CCGG 4 cut(s) 385, 535, 682, 1077
HphI GGTGA 3 cut(s) 568, 686, 995
Hpy166II GTNNAC 2 cut(s) 157, 405
Hpy188I TCNGA 6 cut(s) 88, 393, 501, 647, 931, 1053
Hpy188III TCNNGA 2 cut(s) 458, 548
Hpy8I GTNNAC 2 cut(s) 157, 405
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 8 cut(s) 176, 185, 275, 406, 461, 554, 880, 926
HpyCH4III ACNGT 4 cut(s) 32, 701, 1030, 1125
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 7 cut(s) 453, 540, 585, 678, 834, 993, 1074
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 179, 185, 201, 800, 968, 1109, 1118
HpyF3I CTNAG 5 cut(s) 112, 117, 205, 275, 516
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 9 cut(s) 130, 155, 227, 443, 629, 665, 997, 1026, 1061
HspAI GCGC 2 cut(s) 39, 171
KflI GGGWCCC 1 cut(s) 714
Ksp22I TGATCA 1 cut(s) 1105
Kzo9I GATC 3 cut(s) 88, 1048, 1105
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 5 cut(s) 166, 552, 597, 634, 902
LweI GCATC 4 cut(s) 572, 665, 687, 736
MaeI CTAG 3 cut(s) 251, 458, 1005
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 5 cut(s) 32, 52, 241, 270, 685
MalI GATC 3 cut(s) 90, 1050, 1107
MboI GATC 3 cut(s) 88, 1048, 1105
MboII GAAGA 2 cut(s) 569, 868
MfeI CAATTG 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 332
MluCI AATT 5 cut(s) 375, 600, 690, 766, 988
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 909, 1090
Mph1103I ATGCAT 1 cut(s) 680
MseI TTAA 3 cut(s) 72, 399, 870
MslI CAYNNNNRTG 3 cut(s) 131, 666, 732
MspA1I CMGCKG 3 cut(s) 724, 893, 1103
MspI CCGG 4 cut(s) 385, 535, 682, 1077
MspR9I CCNGG 3 cut(s) 267, 386, 683
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 2 cut(s) 41, 171
MwoI GCNNNNNNNGC 8 cut(s) 45, 179, 185, 201, 800, 968, 1109, 1118
NciI CCSGG 2 cut(s) 386, 683
NcoI CCATGG 2 cut(s) 126, 223
NdeI CATATG 1 cut(s) 524
NdeII GATC 3 cut(s) 88, 1048, 1105
NlaIII CATG 9 cut(s) 130, 155, 227, 443, 629, 665, 997, 1026, 1061
NlaIV GGNNCC 2 cut(s) 715, 716
NmeAIII GCCGAG 1 cut(s) 1075
NmuCI GTSAC 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 680
NspI RCATGY 3 cut(s) 443, 997, 1061
PciI ACATGT 1 cut(s) 1057
PfeI GAWTC 2 cut(s) 741, 812
PinAI ACCGGT 1 cut(s) 534
PkrI GCNGC 8 cut(s) 181, 218, 542, 587, 624, 726, 892, 907
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PpuMI RGGWCCY 2 cut(s) 714, 1034
PscI ACATGT 1 cut(s) 1057
Psp5II RGGWCCY 2 cut(s) 714, 1034
Psp6I CCWGG 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 107
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 2 cut(s) 715, 716
PspPI GGNCC 6 cut(s) 80, 227, 263, 479, 714, 1034
PspPPI RGGWCCY 2 cut(s) 714, 1034
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
RseI CAYNNNNRTG 3 cut(s) 131, 666, 732
SaqAI TTAA 3 cut(s) 72, 399, 870
SatI GCNGC 8 cut(s) 180, 217, 541, 586, 623, 725, 891, 906
Sau3AI GATC 3 cut(s) 88, 1048, 1105
Sau96I GGNCC 6 cut(s) 80, 227, 263, 479, 714, 1034
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 267, 386, 683
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 4 cut(s) 572, 665, 687, 736
SinI GGWCC 5 cut(s) 227, 263, 479, 714, 1034
SmiMI CAYNNNNRTG 3 cut(s) 131, 666, 732
SmlI CTYRAG 1 cut(s) 957
SmoI CTYRAG 1 cut(s) 957
Sse9I AATT 5 cut(s) 375, 600, 690, 766, 988
SsiI CCGC 7 cut(s) 22, 46, 131, 216, 724, 893, 905
SspMI CTAG 3 cut(s) 251, 458, 1005
StyD4I CCNGG 3 cut(s) 265, 384, 681
StyI CCWWGG 2 cut(s) 126, 223
TaaI ACNGT 4 cut(s) 32, 701, 1030, 1125
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 289, 475, 605
TasI AATT 5 cut(s) 375, 600, 690, 766, 988
TatI WGTACW 1 cut(s) 751
TauI GCSGC 3 cut(s) 219, 727, 908
TfiI GAWTC 2 cut(s) 741, 812
Tru1I TTAA 3 cut(s) 72, 399, 870
Tru9I TTAA 3 cut(s) 72, 399, 870
TscAI CASTG 3 cut(s) 704, 811, 1047
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 5 cut(s) 179, 540, 585, 622, 890
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 5 cut(s) 570, 858, 910, 934, 989
TspGWI ACGGA 2 cut(s) 576, 605
TspRI CASTG 3 cut(s) 704, 811, 1047
VpaK11BI GGWCC 5 cut(s) 227, 263, 479, 714, 1034
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 3 cut(s) 443, 997, 1061
XspI CTAG 3 cut(s) 251, 458, 1005
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.