FvH4_6g17090

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011793
Physical Location & Seq
Reverse (-)
11041172 .. 11043725
2554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17090.t1

Sequence Viewer

Length: 1275 bp
ATGAGAACCAAAACAACACTCTTCTTTTGGTTATCTGCATTTGCCATTGCACTTGTGTCCATTACCTTTGCACTCCACAGCTTCTCATCTCTTCCCTCAACTTCTGTTTTCTCCGATTATGTGCAAAGCGGCTTCAATGTTGCCAGTTTTGATGGTTTTTTCAAGCAAAGTGGTGAGACCTTATTGTCAATCGTGAGAAGCATTGTGTCCAGAGTCTCCAGACACCATCAACAGCAGCAGAGGATAAATTGCGATGTGAAAATATGGAAATCGAGGCTCATATCGGAATTCAATGTGTTACTTGTTTTGACTGTTGGCTTAAATGGTTGCACAAACTTTAGTAGTGTGCAAAAGGCAGTAGATGCTGCTCCTGAGCTTAGCACTAATAGGACTCTTATCATAATCGATGCAGGTACTTACAGGGAGAAGGTGACGGTTAATGCTAGCAAGACCAATTTGATATTTCAAGGTCAGGGTTATCTCAACACCGCCATAGCTTGGAATGACTCTGCCAATTCTTCTGGAGGGACTGGCCGAAGCGCTTCTGTTTCTATATTTGCTCCCAGTTTCACTGCCTACAACATTAGCTTTAAGAACACAGCCCCGCCACCATCAGAAGGCAGAATCGGAGCACAAGCAGTGGCACTTCGAGTTACTGGAGATCAGGCTGCATTCTATGGATGTGGTTTCTATGGTGCGCAAGACACACTCAACGATGACCGCGGAAGACATTACTTTAGAGAATGTTTCATTCAAGGATCCATTGATTTCATTTTCGGAAGTGCTAGATCACTATATGAGGGGTGCACAATTAATTCCATAGCAAATCCAGTGAAAGATGGGATTCTACTGAAGGGTAGGATAAGTGGAGCCATCACAGCACAAGGAAGGAAATCGATGCAGGAGCAAAGTGGGTATTCCTTTGTGAATTGCAGCATTGGAGGAAGTGGGAAGGTGTGGCTTGGTCGAGCTTGGGGAGCTTATGCCACTGTGGTGTTCTCCAGAACATACATGTCTGATGTTGTTGCTTCAGATGGTTGGAATGACTGGAGTGATTCTTCTAGAGATGAGAAAGTGTTTTATGGGGAGTACGAGTGCACAGGACCAGGAGCAAATAATACAAATAGGGTCTCATATTCAAAGCAGCTCAATGAATCTGAAGCTGCACCCTTTTTGGATGTTTCCTACATTGATGGGGGTATATGGCTGTCATATACTCCACTCTTCAATCTTCATGAAGATTACTATAATGATCACTTTACTTATACTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

425

Amino Acids

46.59

Weight (kDa)

6.99

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 104 - 399 5.4e-67 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 184
Acc16I TGCGCA 1 cut(s) 699
Acc36I ACCTGC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 498
AccII CGCG 1 cut(s) 723
AciI CCGC 5 cut(s) 129, 489, 605, 721, 723
AclWI GGATC 2 cut(s) 753, 766
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 3 cut(s) 872, 1014, 1179
AfaI GTAC 2 cut(s) 415, 1091
AfeI AGCGCT 1 cut(s) 541
AfiI CCNNNNNNNGG 2 cut(s) 498, 617
AflIII ACRYGT 1 cut(s) 1011
AgsI TTSAA 7 cut(s) 136, 163, 292, 467, 755, 1140, 1228
AjnI CCWGG 1 cut(s) 1105
AleI CACNNNNGTG 1 cut(s) 992
AluBI AGCT 8 cut(s) 81, 376, 497, 588, 971, 980, 1147, 1163
AluI AGCT 8 cut(s) 81, 376, 497, 588, 971, 980, 1147, 1163
Alw21I GWGCWC 3 cut(s) 634, 809, 1100
Alw26I GTCTC 3 cut(s) 170, 220, 1135
Alw44I GTGCAC 2 cut(s) 805, 1096
AlwI GGATC 2 cut(s) 753, 766
Aor51HI AGCGCT 1 cut(s) 541
AoxI GGCC 1 cut(s) 532
ApaLI GTGCAC 2 cut(s) 805, 1096
ApeKI GCWGC 6 cut(s) 235, 365, 668, 933, 1144, 1163
ApoI RAATTY 1 cut(s) 287
AseI ATTAAT 1 cut(s) 813
Asp700I GAANNNNTTC 1 cut(s) 541
AspLEI GCGC 2 cut(s) 542, 700
AspS9I GGNCC 1 cut(s) 1103
AsuHPI GGTGA 2 cut(s) 185, 442
AsuNHI GCTAGC 1 cut(s) 443
AvaII GGWCC 1 cut(s) 1103
BaeGI GKGCMC 2 cut(s) 809, 1100
BamHI GGATCC 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 733
Bbv12I GWGCWC 3 cut(s) 634, 809, 1100
BbvI GCAGC 6 cut(s) 247, 352, 655, 945, 1150, 1156
BccI CCATC 7 cut(s) 146, 234, 619, 833, 881, 1028, 1187
BciT130I CCWGG 1 cut(s) 1107
BclI TGATCA 1 cut(s) 1252
BcoDI GTCTC 3 cut(s) 170, 220, 1135
BfaI CTAG 4 cut(s) 444, 786, 1062, 1273
BfoI RGCGCY 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 401
BisI GCNGC 7 cut(s) 130, 236, 366, 669, 934, 1145, 1164
BlpI GCTNAGC 1 cut(s) 377
BlsI GCNGC 7 cut(s) 131, 237, 367, 670, 935, 1146, 1165
Bme1390I CCNGG 1 cut(s) 1107
Bme18I GGWCC 1 cut(s) 1103
BmgT120I GGNCC 1 cut(s) 1103
BmiI GGNNCC 2 cut(s) 760, 871
BmrFI CCNGG 1 cut(s) 1107
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 3 cut(s) 352, 397, 888
BmtI GCTAGC 1 cut(s) 447
BmuI ACTGGG 1 cut(s) 558
BpiI GAAGAC 1 cut(s) 733
BpmI CTGGAG 5 cut(s) 202, 543, 678, 985, 1069
Bpu10I CCTNAGC 1 cut(s) 372
Bpu1102I GCTNAGC 1 cut(s) 377
Bsa29I ATCGAT 2 cut(s) 405, 896
BsaI GGTCTC 2 cut(s) 170, 1135
BsaJI CCNNGG 1 cut(s) 721
BsaXI ACNNNNNCTCC 2 cut(s) 1079, 1109
Bsc4I CCNNNNNNNGG 2 cut(s) 498, 617
Bse1I ACTGG 6 cut(s) 144, 535, 564, 661, 830, 1052
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 1107
BseCI ATCGAT 2 cut(s) 405, 896
BseDI CCNNGG 1 cut(s) 721
BseGI GGATG 2 cut(s) 686, 1183
BseLI CCNNNNNNNGG 2 cut(s) 498, 617
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 363
BseNI ACTGG 6 cut(s) 144, 535, 564, 661, 830, 1052
BseSI GKGCMC 2 cut(s) 809, 1100
BseXI GCAGC 6 cut(s) 247, 352, 655, 945, 1150, 1156
BsgI GTGCAG 1 cut(s) 1149
Bsh1236I CGCG 1 cut(s) 723
BshFI GGCC 1 cut(s) 534
BshVI ATCGAT 2 cut(s) 405, 896
BsiHKAI GWGCWC 3 cut(s) 634, 809, 1100
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 498, 617
BsmAI GTCTC 3 cut(s) 170, 220, 1135
BsmFI GGGAC 1 cut(s) 541
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 1 cut(s) 534
Bso31I GGTCTC 2 cut(s) 170, 1135
Bsp1286I GDGCHC 3 cut(s) 634, 809, 1100
Bsp143I GATC 4 cut(s) 661, 758, 788, 1252
Bsp1720I GCTNAGC 1 cut(s) 377
BspACI CCGC 5 cut(s) 129, 489, 605, 721, 723
BspANI GGCC 1 cut(s) 534
BspCNI CTCAG 1 cut(s) 364
BspDI ATCGAT 2 cut(s) 405, 896
BspFNI CGCG 1 cut(s) 723
BspHI TCATGA 1 cut(s) 1234
BspLI GGNNCC 2 cut(s) 760, 871
BspMI ACCTGC 1 cut(s) 401
BspOI GCTAGC 1 cut(s) 447
BspPI GGATC 2 cut(s) 753, 766
BspTNI GGTCTC 2 cut(s) 170, 1135
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 6 cut(s) 144, 535, 564, 661, 830, 1052
BssECI CCNNGG 1 cut(s) 721
BssMI GATC 4 cut(s) 661, 758, 788, 1252
Bst2UI CCWGG 1 cut(s) 1107
Bst4CI ACNGT 3 cut(s) 313, 436, 991
Bst6I CTCTTC 3 cut(s) 26, 96, 1229
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 372, 377
BstDSI CCRYGG 1 cut(s) 721
BstF5I GGATG 2 cut(s) 686, 1183
BstFNI CGCG 1 cut(s) 723
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 542, 700
BstKTI GATC 4 cut(s) 664, 761, 791, 1255
BstMAI GTCTC 3 cut(s) 170, 220, 1135
BstMBI GATC 4 cut(s) 661, 758, 788, 1252
BstMWI GCNNNNNNNGC 3 cut(s) 362, 878, 977
BstNI CCWGG 1 cut(s) 1107
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 1 cut(s) 1105
BstSLI GKGCMC 2 cut(s) 809, 1100
BstUI CGCG 1 cut(s) 723
BstV1I GCAGC 6 cut(s) 247, 352, 655, 945, 1150, 1156
BstV2I GAAGAC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 758
BstYI RGATCY 1 cut(s) 758
Bsu15I ATCGAT 2 cut(s) 405, 896
BsuRI GGCC 1 cut(s) 534
BsuTUI ATCGAT 2 cut(s) 405, 896
BtgI CCRYGG 1 cut(s) 721
BtgZI GCGATG 1 cut(s) 267
BtsCI GGATG 2 cut(s) 686, 1183
BtsI GCAGTG 2 cut(s) 570, 645
BtsIMutI CAGTG 4 cut(s) 570, 645, 837, 987
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 1 cut(s) 445
CciI TCATGA 1 cut(s) 1234
CfoI GCGC 2 cut(s) 542, 700
Cfr13I GGNCC 1 cut(s) 1103
Cfr42I CCGCGG 1 cut(s) 724
ClaI ATCGAT 2 cut(s) 405, 896
Csp6I GTAC 2 cut(s) 414, 1090
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 2 cut(s) 1012, 1235
CviQI GTAC 2 cut(s) 414, 1090
DdeI CTNAG 2 cut(s) 372, 377
DpnI GATC 4 cut(s) 663, 760, 790, 1254
DpnII GATC 4 cut(s) 661, 758, 788, 1252
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 26, 96, 1229
EarI CTCTTC 3 cut(s) 26, 96, 1229
Eco31I GGTCTC 2 cut(s) 170, 1135
Eco47I GGWCC 1 cut(s) 1103
Eco47III AGCGCT 1 cut(s) 541
Eco57I CTGAAG 3 cut(s) 872, 1014, 1179
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 1105
FaeI CATG 2 cut(s) 1015, 1238
FaqI GGGAC 1 cut(s) 541
FatI CATG 2 cut(s) 1011, 1234
FauI CCCGC 1 cut(s) 612
FbaI TGATCA 1 cut(s) 1252
Fnu4HI GCNGC 7 cut(s) 130, 236, 366, 669, 934, 1145, 1164
FokI GGATG 2 cut(s) 693, 1190
Fsp4HI GCNGC 7 cut(s) 130, 236, 366, 669, 934, 1145, 1164
FspBI CTAG 4 cut(s) 444, 786, 1062, 1273
FspI TGCGCA 1 cut(s) 699
GlaI GCGC 2 cut(s) 541, 699
GluI GCNGC 7 cut(s) 130, 236, 366, 669, 934, 1145, 1164
GsuI CTGGAG 5 cut(s) 202, 543, 678, 985, 1069
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 1 cut(s) 534
HhaI GCGC 2 cut(s) 542, 700
Hin1II CATG 2 cut(s) 1015, 1238
Hin6I GCGC 2 cut(s) 540, 698
HinP1I GCGC 2 cut(s) 540, 698
HinfI GANTC 7 cut(s) 213, 391, 506, 624, 844, 1055, 1154
HphI GGTGA 2 cut(s) 185, 442
Hpy166II GTNNAC 2 cut(s) 807, 1098
Hpy188I TCNGA 8 cut(s) 115, 286, 616, 629, 779, 1018, 1033, 1159
Hpy188III TCNNGA 8 cut(s) 193, 210, 219, 371, 522, 1002, 1062, 1235
Hpy8I GTNNAC 2 cut(s) 807, 1098
HpyAV CCTTC 5 cut(s) 421, 611, 847, 882, 946
HpyCH4III ACNGT 3 cut(s) 313, 436, 991
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 878, 977
HpyF3I CTNAG 2 cut(s) 372, 377
Hsp92II CATG 2 cut(s) 1015, 1238
HspAI GCGC 2 cut(s) 540, 698
Ksp22I TGATCA 1 cut(s) 1252
KspI CCGCGG 1 cut(s) 724
Kzo9I GATC 4 cut(s) 661, 758, 788, 1252
LmnI GCTCC 7 cut(s) 373, 565, 629, 869, 904, 977, 1109
Lsp1109I GCAGC 6 cut(s) 247, 352, 655, 945, 1150, 1156
LweI GCATC 3 cut(s) 352, 397, 888
MaeI CTAG 4 cut(s) 444, 786, 1062, 1273
MaeIII GTNAC 3 cut(s) 297, 430, 652
MalI GATC 4 cut(s) 663, 760, 790, 1254
MboI GATC 4 cut(s) 661, 758, 788, 1252
MboII GAAGA 8 cut(s) 13, 83, 510, 738, 1050, 1216, 1223, 1250
MflI RGATCY 1 cut(s) 758
MhlI GDGCHC 3 cut(s) 634, 809, 1100
MluCI AATT 7 cut(s) 247, 287, 454, 514, 810, 814, 928
MlyI GAGTC 3 cut(s) 222, 385, 500
MmeI TCCRAC 1 cut(s) 1019
MnlI CCTC 6 cut(s) 106, 234, 267, 518, 793, 935
MroXI GAANNNNTTC 1 cut(s) 541
MseI TTAA 4 cut(s) 320, 438, 591, 813
MslI CAYNNNNRTG 1 cut(s) 992
MspA1I CMGCKG 1 cut(s) 723
MspR9I CCNGG 1 cut(s) 1107
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 1 cut(s) 1107
MvnI CGCG 1 cut(s) 723
MwoI GCNNNNNNNGC 3 cut(s) 362, 878, 977
NdeII GATC 4 cut(s) 661, 758, 788, 1252
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 2 cut(s) 1015, 1238
NlaIV GGNNCC 2 cut(s) 760, 871
NmuCI GTSAC 1 cut(s) 430
NsbI TGCGCA 1 cut(s) 699
NspI RCATGY 1 cut(s) 1015
OliI CACNNNNGTG 1 cut(s) 992
PagI TCATGA 1 cut(s) 1234
PciI ACATGT 1 cut(s) 1011
PctI GAATGC 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 4 cut(s) 624, 844, 1055, 1154
PflMI CCANNNNNTGG 1 cut(s) 498
PkrI GCNGC 7 cut(s) 131, 237, 367, 670, 935, 1146, 1165
PleI GAGTC 3 cut(s) 221, 385, 500
PpsI GAGTC 3 cut(s) 221, 385, 500
PscI ACATGT 1 cut(s) 1011
PshBI ATTAAT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 1105
PspGI CCWGG 1 cut(s) 1105
PspN4I GGNNCC 2 cut(s) 760, 871
PspPI GGNCC 1 cut(s) 1103
PsuI RGATCY 1 cut(s) 758
RsaI GTAC 2 cut(s) 415, 1091
RsaNI GTAC 2 cut(s) 414, 1090
RseI CAYNNNNRTG 1 cut(s) 992
SacII CCGCGG 1 cut(s) 724
SaqAI TTAA 4 cut(s) 320, 438, 591, 813
SatI GCNGC 7 cut(s) 130, 236, 366, 669, 934, 1145, 1164
Sau3AI GATC 4 cut(s) 661, 758, 788, 1252
Sau96I GGNCC 1 cut(s) 1103
SchI GAGTC 3 cut(s) 222, 385, 500
ScrFI CCNGG 1 cut(s) 1107
SduI GDGCHC 3 cut(s) 634, 809, 1100
SfaNI GCATC 3 cut(s) 352, 397, 888
Sfr303I CCGCGG 1 cut(s) 724
SgrBI CCGCGG 1 cut(s) 724
SinI GGWCC 1 cut(s) 1103
SmiMI CAYNNNNRTG 1 cut(s) 992
Sse9I AATT 7 cut(s) 247, 287, 454, 514, 810, 814, 928
SsiI CCGC 5 cut(s) 129, 489, 605, 721, 723
SspMI CTAG 4 cut(s) 444, 786, 1062, 1273
StyD4I CCNGG 1 cut(s) 1105
TaaI ACNGT 3 cut(s) 313, 436, 991
TaqI TCGA 5 cut(s) 272, 405, 649, 896, 967
TasI AATT 7 cut(s) 247, 287, 454, 514, 810, 814, 928
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 4 cut(s) 624, 844, 1055, 1154
Tru1I TTAA 4 cut(s) 320, 438, 591, 813
Tru9I TTAA 4 cut(s) 320, 438, 591, 813
TscAI CASTG 4 cut(s) 577, 645, 837, 994
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 6 cut(s) 235, 365, 668, 933, 1144, 1163
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 5 cut(s) 739, 760, 1167, 1223, 1251
TspRI CASTG 4 cut(s) 577, 645, 837, 994
Van91I CCANNNNNTGG 1 cut(s) 498
VneI GTGCAC 2 cut(s) 805, 1096
VpaK11BI GGWCC 1 cut(s) 1103
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 1 cut(s) 287
XbaI TCTAGA 1 cut(s) 1061
XceI RCATGY 1 cut(s) 1015
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 4 cut(s) 444, 786, 1062, 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.