Rh3BG190300

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011793
Physical Location & Seq
Reverse (-)
16831811 .. 16834397
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG190300.1

Sequence Viewer

Length: 1302 bp
ATGAGAACCAAAACAACATTCTTCTTTTGGCTCTCTGCCTTGGCTATTGCACTAGTTTCCATTACCTTTGCACTCCGCAGCTTCTCATCTCTTTCCTCAACTTCTGTAATCTCCAACTTTGTGCAAACTGGCTCCGATCTTGCCATGTTTGATGTTTTTTTCAAGCAAAGTGTTGAGACCTTATTGTCAACCGTGAGAAGCATTGTGTCCAGACACCATCATCACCAGCAGAGGATAAACTGCGACGAAAAATGGAACTCGAGGCTCATATCCGAATTCAATGTGTCACTTGTTTTGACTGTTGGCTTAAATGGCTGCACCAACTTTAGTAGTGTGCAAAAGGCAGTAGATGCCGTTCCTGAGCTTAGCACTAATAGGACACTCATCATAATCGACTCAGGTACTTACAGGGAGAAGGTGACAGTTAATGTTAGCAAGACCAATTTGATATTTCAAGGTCAGGGTTATCTTAACACTGCCATAGCTTGGAATGACTCGGCCAATTCTTCTGGGGGTACTGGCCGAAGCTCTTCAGTTTCTATATCTGCTCCCAATTTCACTGCCTACAACATTAGCTTTAAGAACACAGCTCCGCCACCATCAAAAGGCAGAATTGGAGCACAGGCAGTGGCACTTCGAGTTTCTGGAGATCAAGCTGCATTTTATGGATGTGGTTTCTATGGGGCGCAAGACACTCTCAATGACGACCGTGGAAGACATTATTTTAGAGAATGTTTCATTCAAGGATCCATAGATTTCATTTTCGGAAGCGCTAGATCACTATATGAGGGGTGCACCATTAATTCAATAGCAAATCCAGTGAAAGGTGGGATTCCACTGAAAGGTAGGATAAGTGGAGCCATCACAGCACAAGGAAGGCAATCAATGCAGGAGCAAAGTGGGTATTCCTTTGTGAATTGCAGTATTGGAGGAAGTGGGAGAGTGTGGCTTGGTCGAGCTTGGGGAGCTTACGCCACTGTGGTTTTCTCCAAAACGTACATGTCTGACGTTGTTGCTTCAGATGGTTGGAATGACTGGAGTGACCCTTCTAGAGATCAGAAAGTGTTTTATGGGGAGTACGAGTGCATAGGACCAGGAGCAAATAATACAAATAGGGTCTCATATTCAAAGCAGCTCAACGAATCTGAGCAGCACCCTTTTTGGATGTTTCCTACATTGATGGGGATGAATGGCTTTTATATACTCCACTCTTCAGTCTTCATGAAGATTACCATATGCACAATTATCAGTTTACTTACTAGTATGTGTAATGTTCCCTCTCCCAGGCGTGTCGCATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

433

Amino Acids

47.53

Weight (kDa)

9.08

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 100 - 377 2.6e-65 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 486
AciI CCGC 2 cut(s) 76, 593
AclWI GGATC 2 cut(s) 741, 754
AcoI YGGCCR 2 cut(s) 498, 520
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 3 cut(s) 516, 1002, 1197
AfaI GTAC 4 cut(s) 403, 517, 998, 1079
AfeI AGCGCT 1 cut(s) 772
AfiI CCNNNNNNNGG 5 cut(s) 486, 605, 824, 842, 1284
AflIII ACRYGT 1 cut(s) 999
AgsI TTSAA 6 cut(s) 163, 280, 455, 743, 807, 1128
AhlI ACTAGT 2 cut(s) 52, 1259
AjnI CCWGG 2 cut(s) 1093, 1283
AjuI GAANNNNNNNTTGG 1 cut(s) 34
Alw21I GWGCWC 2 cut(s) 622, 797
Alw26I GTCTC 2 cut(s) 170, 1123
Alw44I GTGCAC 1 cut(s) 793
AlwI GGATC 2 cut(s) 741, 754
Ama87I CYCGRG 1 cut(s) 259
Aor51HI AGCGCT 1 cut(s) 772
AoxI GGCC 2 cut(s) 498, 520
ApaLI GTGCAC 1 cut(s) 793
ApeKI GCWGC 5 cut(s) 78, 315, 656, 1132, 1150
ApoI RAATTY 1 cut(s) 275
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 529
AspLEI GCGC 2 cut(s) 688, 773
AspS9I GGNCC 1 cut(s) 1091
AsuHPI GGTGA 2 cut(s) 215, 430
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 1 cut(s) 1091
BaeGI GKGCMC 1 cut(s) 797
BamHI GGATCC 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 721, 1210
Bbv12I GWGCWC 2 cut(s) 622, 797
BbvI GCAGC 5 cut(s) 90, 302, 643, 1144, 1162
BccI CCATC 5 cut(s) 225, 607, 869, 1016, 1174
BceAI ACGGC 1 cut(s) 338
BciT130I CCWGG 2 cut(s) 1095, 1285
BcoDI GTCTC 2 cut(s) 170, 1123
BcuI ACTAGT 2 cut(s) 52, 1259
BfaI CTAG 4 cut(s) 53, 774, 1050, 1260
BfoI RGCGCY 1 cut(s) 774
BisI GCNGC 5 cut(s) 79, 316, 657, 1133, 1151
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 5 cut(s) 80, 317, 658, 1134, 1152
Bme1390I CCNGG 2 cut(s) 1095, 1285
Bme18I GGWCC 1 cut(s) 1091
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 1091
BmiI GGNNCC 3 cut(s) 133, 748, 859
BmrFI CCNGG 2 cut(s) 1095, 1285
BmsI GCATC 1 cut(s) 340
BpiI GAAGAC 2 cut(s) 721, 1210
BpmI CTGGAG 2 cut(s) 666, 1057
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 1 cut(s) 365
BsaI GGTCTC 2 cut(s) 170, 1123
BsaJI CCNNGG 3 cut(s) 39, 709, 1283
BsaXI ACNNNNNCTCC 2 cut(s) 1067, 1097
Bsc4I CCNNNNNNNGG 5 cut(s) 486, 605, 824, 842, 1284
Bse1I ACTGG 4 cut(s) 133, 523, 818, 1040
BseBI CCWGG 2 cut(s) 1095, 1285
BseDI CCNNGG 3 cut(s) 39, 709, 1283
BseGI GGATG 3 cut(s) 674, 1170, 1191
BseLI CCNNNNNNNGG 5 cut(s) 486, 605, 824, 842, 1284
BseMII CTCAG 3 cut(s) 351, 411, 1137
BseNI ACTGG 4 cut(s) 133, 523, 818, 1040
BseSI GKGCMC 1 cut(s) 797
BseXI GCAGC 5 cut(s) 90, 302, 643, 1144, 1162
BsgI GTGCAG 1 cut(s) 301
Bsh1285I CGRYCG 1 cut(s) 709
BshFI GGCC 2 cut(s) 500, 522
BsiEI CGRYCG 1 cut(s) 709
BsiHKAI GWGCWC 2 cut(s) 622, 797
BsiHKCI CYCGRG 1 cut(s) 259
BslI CCNNNNNNNGG 5 cut(s) 486, 605, 824, 842, 1284
BsmAI GTCTC 2 cut(s) 170, 1123
BsnI GGCC 2 cut(s) 500, 522
Bso31I GGTCTC 2 cut(s) 170, 1123
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 622, 797
Bsp143I GATC 5 cut(s) 136, 649, 746, 776, 1054
Bsp1720I GCTNAGC 1 cut(s) 365
BspACI CCGC 2 cut(s) 76, 593
BspANI GGCC 2 cut(s) 500, 522
BspCNI CTCAG 3 cut(s) 352, 410, 1138
BspHI TCATGA 1 cut(s) 1221
BspLI GGNNCC 3 cut(s) 133, 748, 859
BspPI GGATC 2 cut(s) 741, 754
BspQI GCTCTTC 1 cut(s) 535
BspTNI GGTCTC 2 cut(s) 170, 1123
BsrI ACTGG 4 cut(s) 133, 523, 818, 1040
BssECI CCNNGG 3 cut(s) 39, 709, 1283
BssMI GATC 5 cut(s) 136, 649, 746, 776, 1054
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 1095, 1285
Bst4CI ACNGT 5 cut(s) 193, 301, 424, 710, 979
Bst6I CTCTTC 2 cut(s) 535, 1216
BstAPI GCANNNNNTGC 2 cut(s) 350, 886
BstDEI CTNAG 4 cut(s) 360, 365, 397, 1146
BstDSI CCRYGG 1 cut(s) 709
BstENI CCTNNNNNAGG 1 cut(s) 1282
BstF5I GGATG 3 cut(s) 674, 1170, 1191
BstH2I RGCGCY 1 cut(s) 774
BstHHI GCGC 2 cut(s) 688, 773
BstKTI GATC 5 cut(s) 139, 652, 749, 779, 1057
BstMAI GTCTC 2 cut(s) 170, 1123
BstMBI GATC 5 cut(s) 136, 649, 746, 776, 1054
BstMCI CGRYCG 1 cut(s) 709
BstMWI GCNNNNNNNGC 5 cut(s) 312, 350, 866, 886, 965
BstNI CCWGG 2 cut(s) 1095, 1285
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 2 cut(s) 1093, 1283
BstSLI GKGCMC 1 cut(s) 797
BstV1I GCAGC 5 cut(s) 90, 302, 643, 1144, 1162
BstV2I GAAGAC 2 cut(s) 721, 1210
BstX2I RGATCY 1 cut(s) 746
BstYI RGATCY 1 cut(s) 746
BsuRI GGCC 2 cut(s) 500, 522
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 3 cut(s) 674, 1170, 1191
BtsI GCAGTG 3 cut(s) 474, 558, 633
BtsIMutI CAGTG 6 cut(s) 474, 558, 633, 825, 836, 975
CciI TCATGA 1 cut(s) 1221
CfoI GCGC 2 cut(s) 688, 773
Cfr13I GGNCC 1 cut(s) 1091
Csp6I GTAC 4 cut(s) 402, 516, 997, 1078
CviAII CATG 3 cut(s) 145, 1000, 1222
CviQI GTAC 4 cut(s) 402, 516, 997, 1078
DdeI CTNAG 4 cut(s) 360, 365, 397, 1146
DpnI GATC 5 cut(s) 138, 651, 748, 778, 1056
DpnII GATC 5 cut(s) 136, 649, 746, 776, 1054
DrdI GACNNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 184
EaeI YGGCCR 2 cut(s) 498, 520
Eam1104I CTCTTC 2 cut(s) 535, 1216
EarI CTCTTC 2 cut(s) 535, 1216
EciI GGCGGA 1 cut(s) 582
Eco130I CCWWGG 1 cut(s) 39
Eco31I GGTCTC 2 cut(s) 170, 1123
Eco47I GGWCC 1 cut(s) 1091
Eco47III AGCGCT 1 cut(s) 772
Eco57I CTGAAG 3 cut(s) 516, 1002, 1197
Eco88I CYCGRG 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 1282
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 1093, 1283
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 3 cut(s) 148, 1003, 1225
FatI CATG 3 cut(s) 144, 999, 1221
FauNDI CATATG 1 cut(s) 1235
Fnu4HI GCNGC 5 cut(s) 79, 316, 657, 1133, 1151
FokI GGATG 3 cut(s) 681, 1177, 1198
Fsp4HI GCNGC 5 cut(s) 79, 316, 657, 1133, 1151
FspBI CTAG 4 cut(s) 53, 774, 1050, 1260
GlaI GCGC 2 cut(s) 687, 772
GluI GCNGC 5 cut(s) 79, 316, 657, 1133, 1151
GsuI CTGGAG 2 cut(s) 666, 1057
HaeII RGCGCY 1 cut(s) 774
HaeIII GGCC 2 cut(s) 500, 522
HhaI GCGC 2 cut(s) 688, 773
Hin1II CATG 3 cut(s) 148, 1003, 1225
Hin6I GCGC 2 cut(s) 686, 771
HinP1I GCGC 2 cut(s) 686, 771
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HinfI GANTC 4 cut(s) 395, 494, 832, 1142
HphI GGTGA 2 cut(s) 215, 430
Hpy166II GTNNAC 3 cut(s) 189, 795, 1253
Hpy188I TCNGA 7 cut(s) 136, 274, 767, 1006, 1021, 1059, 1147
Hpy188III TCNNGA 5 cut(s) 210, 359, 645, 1050, 1222
Hpy8I GTNNAC 3 cut(s) 189, 795, 1253
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 409, 870, 1056
HpyCH4III ACNGT 5 cut(s) 193, 301, 424, 710, 979
HpyCH4IV ACGT 2 cut(s) 995, 1008
HpyF10VI GCNNNNNNNGC 5 cut(s) 312, 350, 866, 886, 965
HpyF3I CTNAG 4 cut(s) 360, 365, 397, 1146
HpySE526I ACGT 2 cut(s) 995, 1008
Hsp92II CATG 3 cut(s) 148, 1003, 1225
HspAI GCGC 2 cut(s) 686, 771
Kzo9I GATC 5 cut(s) 136, 649, 746, 776, 1054
LguI GCTCTTC 1 cut(s) 535
LmnI GCTCC 8 cut(s) 137, 553, 595, 617, 857, 892, 965, 1097
Lsp1109I GCAGC 5 cut(s) 90, 302, 643, 1144, 1162
LweI GCATC 1 cut(s) 340
MaeI CTAG 4 cut(s) 53, 774, 1050, 1260
MaeII ACGT 2 cut(s) 995, 1008
MaeIII GTNAC 3 cut(s) 285, 418, 1040
MalI GATC 5 cut(s) 138, 651, 748, 778, 1056
MboI GATC 5 cut(s) 136, 649, 746, 776, 1054
MboII GAAGA 7 cut(s) 13, 498, 522, 726, 1203, 1210, 1237
MflI RGATCY 1 cut(s) 746
MhlI GDGCHC 2 cut(s) 622, 797
MluCI AATT 8 cut(s) 275, 442, 502, 553, 612, 802, 916, 1242
MlyI GAGTC 2 cut(s) 389, 488
MmeI TCCRAC 2 cut(s) 138, 1007
MnlI CCTC 6 cut(s) 106, 225, 255, 781, 923, 1288
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 5 cut(s) 308, 426, 471, 579, 801
MspR9I CCNGG 2 cut(s) 1095, 1285
MvaI CCWGG 2 cut(s) 1095, 1285
MwoI GCNNNNNNNGC 5 cut(s) 312, 350, 866, 886, 965
NdeI CATATG 1 cut(s) 1235
NdeII GATC 5 cut(s) 136, 649, 746, 776, 1054
NlaIII CATG 3 cut(s) 148, 1003, 1225
NlaIV GGNNCC 3 cut(s) 133, 748, 859
NmeAIII GCCGAG 1 cut(s) 476
NmuCI GTSAC 3 cut(s) 285, 418, 1040
NspI RCATGY 1 cut(s) 1003
PaeR7I CTCGAG 1 cut(s) 259
PagI TCATGA 1 cut(s) 1221
PciI ACATGT 1 cut(s) 999
PciSI GCTCTTC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 2 cut(s) 832, 1142
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 5 cut(s) 80, 317, 658, 1134, 1152
PleI GAGTC 2 cut(s) 389, 488
PpsI GAGTC 2 cut(s) 389, 488
PscI ACATGT 1 cut(s) 999
PshBI ATTAAT 1 cut(s) 801
Psp6I CCWGG 2 cut(s) 1093, 1283
PspGI CCWGG 2 cut(s) 1093, 1283
PspN4I GGNNCC 3 cut(s) 133, 748, 859
PspPI GGNCC 1 cut(s) 1091
PspXI VCTCGAGB 1 cut(s) 259
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
PsuI RGATCY 1 cut(s) 746
RsaI GTAC 4 cut(s) 403, 517, 998, 1079
RsaNI GTAC 4 cut(s) 402, 516, 997, 1078
SapI GCTCTTC 1 cut(s) 535
SaqAI TTAA 5 cut(s) 308, 426, 471, 579, 801
SatI GCNGC 5 cut(s) 79, 316, 657, 1133, 1151
Sau3AI GATC 5 cut(s) 136, 649, 746, 776, 1054
Sau96I GGNCC 1 cut(s) 1091
SchI GAGTC 2 cut(s) 389, 488
ScrFI CCNGG 2 cut(s) 1095, 1285
SduI GDGCHC 2 cut(s) 622, 797
SfaNI GCATC 1 cut(s) 340
Sfr274I CTCGAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 1091
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
SpeI ACTAGT 2 cut(s) 52, 1259
Sse9I AATT 8 cut(s) 275, 442, 502, 553, 612, 802, 916, 1242
SsiI CCGC 2 cut(s) 76, 593
SspMI CTAG 4 cut(s) 53, 774, 1050, 1260
StyD4I CCNGG 2 cut(s) 1093, 1283
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 5 cut(s) 193, 301, 424, 710, 979
TaiI ACGT 2 cut(s) 998, 1011
TaqI TCGA 4 cut(s) 260, 393, 637, 955
TasI AATT 8 cut(s) 275, 442, 502, 553, 612, 802, 916, 1242
TfiI GAWTC 2 cut(s) 832, 1142
Tru1I TTAA 5 cut(s) 308, 426, 471, 579, 801
Tru9I TTAA 5 cut(s) 308, 426, 471, 579, 801
TscAI CASTG 6 cut(s) 481, 565, 633, 825, 843, 982
TseFI GTSAC 3 cut(s) 285, 418, 1040
TseI GCWGC 5 cut(s) 78, 315, 656, 1132, 1150
Tsp45I GTSAC 3 cut(s) 285, 418, 1040
TspDTI ATGAA 5 cut(s) 727, 748, 1202, 1210, 1238
TspRI CASTG 6 cut(s) 481, 565, 633, 825, 843, 982
Van91I CCANNNNNTGG 1 cut(s) 486
VneI GTGCAC 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 1091
VspI ATTAAT 1 cut(s) 801
XagI CCTNNNNNAGG 1 cut(s) 1282
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 1 cut(s) 1049
XceI RCATGY 1 cut(s) 1003
XhoI CTCGAG 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 4 cut(s) 53, 774, 1050, 1260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.