FvH4_6g39480

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011793
Physical Location & Seq
Reverse (-)
31173022 .. 31174650
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39480.t1

Sequence Viewer

Length: 1074 bp
ATGTGTCTGAAAACAACATGTTTAACATGTTTTGTTGCCCTTCTTGTTGCCCTTCTTGCAGTTCTATTCGCATCTGCACATCTTACCACTAACCAAAACTCACCATCTGCTCTCAGCATCCCATATGCTGCGCATCATAAACATGAAAATGATGATCCTCTCCCAGACACAACTGCAACCTCCTATCTTTGCGTCGATCAAAACGGGCATTTGAGAAAGTGGAGGTTCCGATTAGCAAACAAACCAAATATAACATTTCAAGGAGAAGGGTATTTATGGACTGTAATTGCTTGGAATGACACGGCCAAGTCATCATATAGCACAAAACTAAGTGCCTCTGTTCATGTTGCTGGTGCCAACTTCATTGCTAAGAACATAAGCTTCAAGAATACGGCTCCACCTCCAGAACCTGGTGTTGATGATGCTCAAGCAGTAGCGCTTAGAGTATCGGGAGATAAAGCTGCATTTTGGGGTTGTGGATTCTATGGATACCAAGACACCCTCAATGATGAGAATGGTCGCCATTACTTCAAGGATTGCTATATCCAGGGCACCGTTGATTACATCTATGGCAATGGACAATCATTCTATGAGAATTGCCAGCTGATCACCATCGCAGCCGCCGGATCAATTGCGTATATTACAGCCCACGGCAGAAGCAATACCGTCAGGGTTGAGAATACGTGTGCTGGCTTTGCCTTTGTTAGTTGCACCATCGATGGGAGTGGAATAGTCTGGTTAGGTCGAGAATGTAGGCGTTTTGCACGAGTAGTTTTTGCCAACACCTACATGAGTAACATTATCACTCCAGAAGGTTGGAATGCAACAACATCCGGAGATGAAAATATAGGCATGCATGGCAGGACTGTGTTCTTTGGAGAATACAAATGCAAGAGTCCAGGAGCTAATATGAACAAGCGTAAACCTTTCGTGCACAATCTCACTCACAGTCAAGCTTATTCTCATTTCCTTACTGTGTCTTTCATCGACGGGGTTGAATGCCTGAGTGGCTGCTACCCTTTAATAATTAGCCTTCTCTCTTTTACGCTCTCTCTAGCTAGCTATGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

358

Amino Acids

39.19

Weight (kDa)

7.55

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 80 - 331 1.8e-48 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 132
AccB1I GGYRCC 2 cut(s) 353, 551
AccB7I CCANNNNNTGG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 833
AciI CCGC 1 cut(s) 621
AclWI GGATC 2 cut(s) 149, 634
AcoI YGGCCR 1 cut(s) 303
AfeI AGCGCT 1 cut(s) 438
AfiI CCNNNNNNNGG 2 cut(s) 410, 720
AflIII ACRYGT 3 cut(s) 17, 26, 683
AgsI TTSAA 4 cut(s) 260, 385, 532, 998
AjnI CCWGG 3 cut(s) 409, 546, 898
AluBI AGCT 7 cut(s) 381, 461, 604, 905, 956, 1058, 1062
AluI AGCT 7 cut(s) 381, 461, 604, 905, 956, 1058, 1062
Alw21I GWGCWC 1 cut(s) 936
Alw44I GTGCAC 1 cut(s) 932
AlwI GGATC 2 cut(s) 149, 634
AlwNI CAGNNNCTG 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 833
Aor51HI AGCGCT 1 cut(s) 438
AoxI GGCC 1 cut(s) 303
ApaLI GTGCAC 1 cut(s) 932
ApeKI GCWGC 4 cut(s) 128, 461, 617, 1011
AspLEI GCGC 2 cut(s) 133, 439
AsuHPI GGTGA 2 cut(s) 93, 601
AsuNHI GCTAGC 1 cut(s) 1058
BaeGI GKGCMC 2 cut(s) 554, 936
BanI GGYRCC 2 cut(s) 353, 551
BauI CACGAG 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 4 cut(s) 115, 448, 629, 998
BccI CCATC 4 cut(s) 112, 620, 713, 722
BceAI ACGGC 3 cut(s) 318, 408, 667
BciT130I CCWGG 3 cut(s) 411, 548, 900
BciVI GTATCC 1 cut(s) 482
BclI TGATCA 1 cut(s) 606
BfaI CTAG 2 cut(s) 1055, 1059
BfoI RGCGCY 1 cut(s) 440
BfuI GTATCC 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 1008
BisI GCNGC 5 cut(s) 129, 462, 618, 621, 1012
BlsI GCNGC 5 cut(s) 130, 463, 619, 622, 1013
Bme1390I CCNGG 3 cut(s) 411, 548, 900
BmiI GGNNCC 4 cut(s) 227, 355, 396, 553
BmrFI CCNGG 3 cut(s) 411, 548, 900
BmsI GCATC 4 cut(s) 80, 126, 142, 412
BmtI GCTAGC 1 cut(s) 1062
BpmI CTGGAG 2 cut(s) 387, 792
BpuEI CTTGAG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 717
BsaAI YACGTR 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 547, 649
BsaWI WCCGGW 1 cut(s) 833
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 720
Bse3DI GCAATG 2 cut(s) 363, 580
Bse8I GATNNNNATC 1 cut(s) 611
BseAI TCCGGA 1 cut(s) 833
BseBI CCWGG 3 cut(s) 411, 548, 900
BseCI ATCGAT 1 cut(s) 717
BseDI CCNNGG 2 cut(s) 547, 649
BseGI GGATG 2 cut(s) 117, 830
BseJI GATNNNNATC 1 cut(s) 611
BseLI CCNNNNNNNGG 2 cut(s) 410, 720
BseMI GCAATG 2 cut(s) 363, 580
BseMII CTCAG 2 cut(s) 127, 995
BseSI GKGCMC 2 cut(s) 554, 936
BseXI GCAGC 4 cut(s) 115, 448, 629, 998
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 2 cut(s) 353, 551
BshVI ATCGAT 1 cut(s) 717
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 2 cut(s) 624, 834
BslI CCNNNNNNNGG 2 cut(s) 410, 720
BsmI GAATGC 2 cut(s) 826, 1004
BsnI GGCC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 554, 936
Bsp13I TCCGGA 1 cut(s) 833
Bsp143I GATC 4 cut(s) 154, 196, 606, 626
BspACI CCGC 1 cut(s) 621
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 2 cut(s) 126, 996
BspDI ATCGAT 1 cut(s) 717
BspEI TCCGGA 1 cut(s) 833
BspLI GGNNCC 4 cut(s) 227, 355, 396, 553
BspOI GCTAGC 1 cut(s) 1062
BspPI GGATC 2 cut(s) 149, 634
BspT107I GGYRCC 2 cut(s) 353, 551
BsrDI GCAATG 2 cut(s) 363, 580
BssECI CCNNGG 2 cut(s) 547, 649
BssMI GATC 4 cut(s) 154, 196, 606, 626
BssSI CACGAG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 3 cut(s) 411, 548, 900
Bst4CI ACNGT 6 cut(s) 283, 556, 667, 868, 950, 976
BstBAI YACGTR 1 cut(s) 684
BstC8I GCNNGC 4 cut(s) 602, 691, 854, 1060
BstDEI CTNAG 5 cut(s) 113, 329, 369, 440, 1004
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 2 cut(s) 117, 830
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 2 cut(s) 133, 439
BstKTI GATC 4 cut(s) 157, 199, 609, 629
BstMBI GATC 4 cut(s) 154, 196, 606, 626
BstMWI GCNNNNNNNGC 4 cut(s) 56, 695, 858, 1008
BstNI CCWGG 3 cut(s) 411, 548, 900
BstNSI RCATGY 3 cut(s) 21, 30, 856
BstSCI CCNGG 3 cut(s) 409, 546, 898
BstSLI GKGCMC 2 cut(s) 554, 936
BstV1I GCAGC 4 cut(s) 115, 448, 629, 998
BstXI CCANNNNNNTGG 1 cut(s) 816
Bsu15I ATCGAT 1 cut(s) 717
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 1 cut(s) 717
BtgI CCRYGG 1 cut(s) 649
BtgZI GCGATG 1 cut(s) 598
BtsCI GGATG 2 cut(s) 117, 830
Cac8I GCNNGC 4 cut(s) 602, 691, 854, 1060
CaiI CAGNNNCTG 1 cut(s) 410
CfoI GCGC 2 cut(s) 133, 439
ClaI ATCGAT 1 cut(s) 717
CseI GACGC 1 cut(s) 181
CsiI ACCWGGT 1 cut(s) 409
CviAII CATG 7 cut(s) 18, 27, 143, 344, 790, 853, 857
DdeI CTNAG 5 cut(s) 113, 329, 369, 440, 1004
DpnI GATC 4 cut(s) 156, 198, 608, 628
DpnII GATC 4 cut(s) 154, 196, 606, 626
EaeI YGGCCR 1 cut(s) 303
Eco47III AGCGCT 1 cut(s) 438
EcoRII CCWGG 3 cut(s) 409, 546, 898
EcoT22I ATGCAT 1 cut(s) 858
FaeI CATG 7 cut(s) 21, 30, 146, 347, 793, 856, 860
FatI CATG 7 cut(s) 17, 26, 142, 343, 789, 852, 856
FauNDI CATATG 1 cut(s) 124
FbaI TGATCA 1 cut(s) 606
Fnu4HI GCNGC 5 cut(s) 129, 462, 618, 621, 1012
FokI GGATG 2 cut(s) 104, 817
Fsp4HI GCNGC 5 cut(s) 129, 462, 618, 621, 1012
FspBI CTAG 2 cut(s) 1055, 1059
FspI TGCGCA 1 cut(s) 132
GlaI GCGC 2 cut(s) 132, 438
GluI GCNGC 5 cut(s) 129, 462, 618, 621, 1012
GsuI CTGGAG 2 cut(s) 387, 792
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 1 cut(s) 305
HapII CCGG 2 cut(s) 624, 834
HgaI GACGC 1 cut(s) 181
HhaI GCGC 2 cut(s) 133, 439
Hin1II CATG 7 cut(s) 21, 30, 146, 347, 793, 856, 860
Hin6I GCGC 2 cut(s) 131, 437
HinP1I GCGC 2 cut(s) 131, 437
HindIII AAGCTT 2 cut(s) 379, 954
HinfI GANTC 2 cut(s) 480, 895
HpaII CCGG 2 cut(s) 624, 834
HphI GGTGA 2 cut(s) 93, 601
Hpy166II GTNNAC 2 cut(s) 923, 934
Hpy188I TCNGA 2 cut(s) 9, 230
Hpy188III TCNNGA 6 cut(s) 385, 404, 450, 746, 809, 834
Hpy8I GTNNAC 2 cut(s) 923, 934
Hpy99I CGWCG 2 cut(s) 197, 992
HpyAV CCTTC 5 cut(s) 50, 62, 260, 806, 1043
HpyCH4III ACNGT 6 cut(s) 283, 556, 667, 868, 950, 976
HpyCH4IV ACGT 1 cut(s) 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 695, 858, 1008
HpyF3I CTNAG 5 cut(s) 113, 329, 369, 440, 1004
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 7 cut(s) 21, 30, 146, 347, 793, 856, 860
HspAI GCGC 2 cut(s) 131, 437
Kpn2I TCCGGA 1 cut(s) 833
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 4 cut(s) 154, 196, 606, 626
LmnI GCTCC 2 cut(s) 400, 902
Lsp1109I GCAGC 4 cut(s) 115, 448, 629, 998
LweI GCATC 4 cut(s) 80, 126, 142, 412
MabI ACCWGGT 1 cut(s) 409
MaeI CTAG 2 cut(s) 1055, 1059
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 1 cut(s) 794
MalI GATC 4 cut(s) 156, 198, 608, 628
MboI GATC 4 cut(s) 154, 196, 606, 626
MfeI CAATTG 1 cut(s) 630
MhlI GDGCHC 2 cut(s) 554, 936
MluCI AATT 4 cut(s) 285, 595, 630, 1026
MlyI GAGTC 1 cut(s) 904
MmeI TCCRAC 1 cut(s) 797
MnlI CCTC 6 cut(s) 168, 190, 216, 346, 411, 512
Mph1103I ATGCAT 1 cut(s) 858
MroI TCCGGA 1 cut(s) 833
MseI TTAA 2 cut(s) 23, 1022
MslI CAYNNNNRTG 3 cut(s) 141, 147, 788
MspA1I CMGCKG 1 cut(s) 604
MspI CCGG 2 cut(s) 624, 834
MspR9I CCNGG 3 cut(s) 411, 548, 900
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 2 cut(s) 826, 1004
MvaI CCWGG 3 cut(s) 411, 548, 900
MwoI GCNNNNNNNGC 4 cut(s) 56, 695, 858, 1008
NdeI CATATG 1 cut(s) 124
NdeII GATC 4 cut(s) 154, 196, 606, 626
NheI GCTAGC 1 cut(s) 1058
NlaIII CATG 7 cut(s) 21, 30, 146, 347, 793, 856, 860
NlaIV GGNNCC 4 cut(s) 227, 355, 396, 553
NsbI TGCGCA 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 3 cut(s) 21, 30, 856
PaeI GCATGC 1 cut(s) 856
PciI ACATGT 2 cut(s) 17, 26
PctI GAATGC 2 cut(s) 826, 1004
PfeI GAWTC 1 cut(s) 480
PflMI CCANNNNNTGG 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 898
PkrI GCNGC 5 cut(s) 130, 463, 619, 622, 1013
PleI GAGTC 1 cut(s) 903
PpsI GAGTC 1 cut(s) 903
Ppu21I YACGTR 1 cut(s) 684
PscI ACATGT 2 cut(s) 17, 26
Psp6I CCWGG 3 cut(s) 409, 546, 898
PspGI CCWGG 3 cut(s) 409, 546, 898
PspN4I GGNNCC 4 cut(s) 227, 355, 396, 553
PstNI CAGNNNCTG 1 cut(s) 410
PvuII CAGCTG 1 cut(s) 604
RseI CAYNNNNRTG 3 cut(s) 141, 147, 788
SaqAI TTAA 2 cut(s) 23, 1022
SatI GCNGC 5 cut(s) 129, 462, 618, 621, 1012
Sau3AI GATC 4 cut(s) 154, 196, 606, 626
SchI GAGTC 1 cut(s) 904
ScrFI CCNGG 3 cut(s) 411, 548, 900
SduI GDGCHC 2 cut(s) 554, 936
SexAI ACCWGGT 1 cut(s) 409
SfaNI GCATC 4 cut(s) 80, 126, 142, 412
SmiMI CAYNNNNRTG 3 cut(s) 141, 147, 788
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SphI GCATGC 1 cut(s) 856
Sse9I AATT 4 cut(s) 285, 595, 630, 1026
SsiI CCGC 1 cut(s) 621
SspMI CTAG 2 cut(s) 1055, 1059
StyD4I CCNGG 3 cut(s) 409, 546, 898
TaaI ACNGT 6 cut(s) 283, 556, 667, 868, 950, 976
TaiI ACGT 1 cut(s) 686
TaqI TCGA 4 cut(s) 195, 717, 745, 987
TasI AATT 4 cut(s) 285, 595, 630, 1026
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 2 cut(s) 23, 1022
Tru9I TTAA 2 cut(s) 23, 1022
TseI GCWGC 4 cut(s) 128, 461, 617, 1011
TspDTI ATGAA 6 cut(s) 159, 332, 352, 855, 926, 973
Van91I CCANNNNNTGG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 932
XceI RCATGY 3 cut(s) 21, 30, 856
XspI CTAG 2 cut(s) 1055, 1059
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.