MD12G1104100.v1.1

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011793
Physical Location & Seq
Forward (+)
16395388 .. 16397324
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1104100.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGACAACCAAAACAACCCTTTTCTTCTGGCTCTCAGCCTTTGCCATTGCACTCCTTTCCACTTTTAAATGCCTCCCCCTCACCCCCTTTCTTTCCCCAACTTCCATTATTTCCGAAAGTGCTCGAAACAGCTTCAAGCTTGCCATATTTGATCAGGTTTTCGAGCAAACCGGTGAGAGCTTACTGTCCACCCTAACAAGCATTGTGGCTAGACACCACCACCACCGTCAGCGTAGGCATACATGTGACCAGACCAAACGGAAAAACTCTAGATTCATGTCCGAATACAATGTATCCCTTGTTTTGACAGTAGGCTTCAAAGGTTGCTCAAATTTTAGTAGTGTCCAAAATGCGGTTGATGCTGTTCCGGAGCTTAGTATTAATAGAACCCTCATCATAATCAACTCCGGCATTTACAGGGAAAAGGTGACAGTGAGTGCTAACAAGACCAACTTGATAATTCAAGGACAAGGTTATCTAAACACTATCATAGCATGGAATGACACTGCAAATTCTACTGGAGGGACTGCTTTTAGCTCTTCAGTTGCCATCTTTGCTCCTTGTTTCACTGCCTACAACATTAGCTTTCAGAACACAGCTCCGCCGCCAGCTCCGGGAAGAATTGGAGCACAGGCAGTGGCCCTGAGAATTGCAGGAGACCAAGCTGCATTTTATGGGTGTGGCTTTTATGGTGCACAAGACACGCTTAATGATGATCGTGGGAGACATTATTTTAGAGAATGTTTCATTCAGGGCTCCATTGATTTCATTTTTGGAAAGGCTAGATCACTATATGAGGGGTGCATCCTTAACTCCATAGCAAATGCAGTCAAATCGGGGATAAGCGGAGCCATCACGGCTCAAGGAAGGCAGTCGATGCAGGAGCAAAGCGGGTTTTCCTTTGTGAATTGTAGCGTTGGAGGAAGTGGGACAGTGTGGCTTGGTCGAGCATGGGGAGCTTATGCGACTGTTGTGTTTTCGAAAACATACATGTCTGATGTTGTTGCTTCAGATGGATGGAATGACTGGAAAGACCCCTCCAGAGACCCGACGGTATTATTTGGGGAGTACGATTGCATGGGGTCCGGTGCGAATTATACATATAGGGTTTCGTATGGAAGGCAACTGAATGAATCTGAAGCAGCTCCTTTCTTGGACATTTCGTACATTGATGGAAATGATTGGCTTTTACATCCTCCACTCTTCAACCTTCATGATGATCACAATCTCAACGATCAATGGACTCGTACATTCAAGTATTTTCGTAGCGTTTTTATTTATCAAACATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

431

Amino Acids

47.64

Weight (kDa)

8.02

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 103 - 393 2.1e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 5 cut(s) 353, 602, 605, 846, 891
AcsI RAATTY 2 cut(s) 331, 511
AcuI CTGAAG 3 cut(s) 525, 993, 1158
AfaI GTAC 3 cut(s) 1070, 1166, 1249
AfiI CCNNNNNNNGG 2 cut(s) 352, 614
AflIII ACRYGT 2 cut(s) 242, 990
AgeI ACCGGT 1 cut(s) 170
AgsI TTSAA 5 cut(s) 136, 319, 464, 1207, 1255
Alw21I GWGCWC 3 cut(s) 124, 631, 697
Alw26I GTCTC 3 cut(s) 651, 718, 1038
Alw44I GTGCAC 1 cut(s) 693
Aor13HI TCCGGA 1 cut(s) 367
AoxI GGCC 1 cut(s) 639
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 2 cut(s) 665, 1142
ApoI RAATTY 2 cut(s) 331, 511
AseI ATTAAT 1 cut(s) 381
AsiGI ACCGGT 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 640, 1083
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 3 cut(s) 73, 185, 439
AsuII TTCGAA 1 cut(s) 980
AvaII GGWCC 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 758
Bbv12I GWGCWC 3 cut(s) 124, 631, 697
BbvI GCAGC 2 cut(s) 652, 1154
BccI CCATC 5 cut(s) 557, 860, 1007, 1011, 1166
BceAI ACGGC 1 cut(s) 873
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 2 cut(s) 151, 1219
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 3 cut(s) 651, 718, 1038
BfaI CTAG 3 cut(s) 210, 270, 783
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 857
BisI GCNGC 3 cut(s) 605, 666, 1143
BlsI GCNGC 3 cut(s) 606, 667, 1144
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 1 cut(s) 1083
BmgT120I GGNCC 2 cut(s) 640, 1083
BmiI GGNNCC 3 cut(s) 757, 850, 1084
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 3 cut(s) 349, 813, 867
BpmI CTGGAG 2 cut(s) 540, 1024
Bpu14I TTCGAA 1 cut(s) 980
BpuEI CTTGAG 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 1224
BsaI GGTCTC 2 cut(s) 651, 1038
BsaWI WCCGGW 3 cut(s) 170, 367, 1085
Bsc4I CCNNNNNNNGG 2 cut(s) 352, 614
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 523, 1031
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 1224
BseAI TCCGGA 1 cut(s) 367
BseGI GGATG 3 cut(s) 804, 1022, 1192
BseJI GATNNNNATC 1 cut(s) 1224
BseLI CCNNNNNNNGG 2 cut(s) 352, 614
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 48, 635
BseNI ACTGG 2 cut(s) 523, 1031
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 2 cut(s) 652, 1154
BshFI GGCC 1 cut(s) 641
BshTI ACCGGT 1 cut(s) 170
BsiHKAI GWGCWC 3 cut(s) 124, 631, 697
BsiSI CCGG 5 cut(s) 171, 368, 408, 614, 1086
BslFI GGGAC 2 cut(s) 538, 943
BslI CCNNNNNNNGG 2 cut(s) 352, 614
BsmAI GTCTC 3 cut(s) 651, 718, 1038
BsmFI GGGAC 2 cut(s) 538, 943
BsnI GGCC 1 cut(s) 641
Bso31I GGTCTC 2 cut(s) 651, 1038
Bsp119I TTCGAA 1 cut(s) 980
Bsp1286I GDGCHC 4 cut(s) 124, 631, 697, 758
Bsp13I TCCGGA 1 cut(s) 367
Bsp143I GATC 5 cut(s) 151, 715, 785, 1219, 1234
BspACI CCGC 5 cut(s) 353, 602, 605, 846, 891
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 2 cut(s) 47, 636
BspEI TCCGGA 1 cut(s) 367
BspHI TCATGA 1 cut(s) 1213
BspLI GGNNCC 3 cut(s) 757, 850, 1084
BspQI GCTCTTC 1 cut(s) 544
BspT104I TTCGAA 1 cut(s) 980
BspTNI GGTCTC 2 cut(s) 651, 1038
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 2 cut(s) 523, 1031
BssAI RCCGGY 1 cut(s) 170
BssMI GATC 5 cut(s) 151, 715, 785, 1219, 1234
Bst4CI ACNGT 7 cut(s) 186, 227, 310, 433, 934, 970, 1054
Bst6I CTCTTC 2 cut(s) 544, 1208
BstAPI GCANNNNNTGC 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 141, 609
BstDEI CTNAG 3 cut(s) 34, 374, 644
BstF5I GGATG 3 cut(s) 804, 1022, 1192
BstKTI GATC 5 cut(s) 154, 718, 788, 1222, 1237
BstMAI GTCTC 3 cut(s) 651, 718, 1038
BstMBI GATC 5 cut(s) 151, 715, 785, 1219, 1234
BstMWI GCNNNNNNNGC 5 cut(s) 359, 554, 857, 877, 956
BstNSI RCATGY 2 cut(s) 246, 994
BstSCI CCNGG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 697
BstV1I GCAGC 2 cut(s) 652, 1154
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 1 cut(s) 641
BtsCI GGATG 3 cut(s) 804, 1022, 1192
BtsI GCAGTG 3 cut(s) 504, 567, 642
BtsIMutI CAGTG 5 cut(s) 438, 504, 567, 642, 939
Cac8I GCNNGC 2 cut(s) 141, 609
CciI TCATGA 1 cut(s) 1213
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 640, 1083
Csp6I GTAC 3 cut(s) 1069, 1165, 1248
CspAI ACCGGT 1 cut(s) 170
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 7 cut(s) 243, 277, 495, 951, 991, 1078, 1214
CviQI GTAC 3 cut(s) 1069, 1165, 1248
DdeI CTNAG 3 cut(s) 34, 374, 644
DpnI GATC 5 cut(s) 153, 717, 787, 1221, 1236
DpnII GATC 5 cut(s) 151, 715, 785, 1219, 1234
DraI TTTAAA 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 544, 1208
EarI CTCTTC 2 cut(s) 544, 1208
EciI GGCGGA 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 758
Eco31I GGTCTC 2 cut(s) 651, 1038
Eco47I GGWCC 1 cut(s) 1083
Eco57I CTGAAG 3 cut(s) 525, 993, 1158
EcoT38I GRGCYC 1 cut(s) 758
FaeI CATG 7 cut(s) 246, 280, 498, 954, 994, 1081, 1217
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 690, 722
FaqI GGGAC 2 cut(s) 538, 943
FatI CATG 7 cut(s) 242, 276, 494, 950, 990, 1077, 1213
FauI CCCGC 1 cut(s) 884
FbaI TGATCA 2 cut(s) 151, 1219
Fnu4HI GCNGC 3 cut(s) 605, 666, 1143
FokI GGATG 3 cut(s) 791, 1029, 1179
FriOI GRGCYC 1 cut(s) 758
Fsp4HI GCNGC 3 cut(s) 605, 666, 1143
FspBI CTAG 3 cut(s) 210, 270, 783
GluI GCNGC 3 cut(s) 605, 666, 1143
GsuI CTGGAG 2 cut(s) 540, 1024
HaeIII GGCC 1 cut(s) 641
HapII CCGG 5 cut(s) 171, 368, 408, 614, 1086
Hin1II CATG 7 cut(s) 246, 280, 498, 954, 994, 1081, 1217
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 3 cut(s) 273, 1133, 1243
HpaII CCGG 5 cut(s) 171, 368, 408, 614, 1086
HphI GGTGA 3 cut(s) 73, 185, 439
Hpy166II GTNNAC 2 cut(s) 189, 695
Hpy188I TCNGA 7 cut(s) 115, 283, 591, 997, 1012, 1138, 1292
Hpy188III TCNNGA 4 cut(s) 270, 368, 1041, 1214
Hpy8I GTNNAC 2 cut(s) 189, 695
Hpy99I CGWCG 1 cut(s) 1054
HpyAV CCTTC 3 cut(s) 861, 1113, 1220
HpyCH4III ACNGT 7 cut(s) 186, 227, 310, 433, 934, 970, 1054
HpyCH4V TGCA 9 cut(s) 50, 509, 653, 668, 695, 804, 827, 880, 1077
HpyF10VI GCNNNNNNNGC 5 cut(s) 359, 554, 857, 877, 956
HpyF3I CTNAG 3 cut(s) 34, 374, 644
Hsp92II CATG 7 cut(s) 246, 280, 498, 954, 994, 1081, 1217
Kpn2I TCCGGA 1 cut(s) 367
Ksp22I TGATCA 2 cut(s) 151, 1219
Kzo9I GATC 5 cut(s) 151, 715, 785, 1219, 1234
LguI GCTCTTC 1 cut(s) 544
Lsp1109I GCAGC 2 cut(s) 652, 1154
LweI GCATC 3 cut(s) 349, 813, 867
MaeI CTAG 3 cut(s) 210, 270, 783
MaeIII GTNAC 2 cut(s) 245, 427
MalI GATC 5 cut(s) 153, 717, 787, 1221, 1236
MboI GATC 5 cut(s) 151, 715, 785, 1219, 1234
MboII GAAGA 4 cut(s) 16, 531, 630, 1195
MhlI GDGCHC 4 cut(s) 124, 631, 697, 758
MluCI AATT 7 cut(s) 331, 459, 511, 621, 648, 907, 1093
MlyI GAGTC 1 cut(s) 1237
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 8 cut(s) 83, 89, 401, 515, 790, 914, 1048, 1206
MroI TCCGGA 1 cut(s) 367
MseI TTAA 4 cut(s) 66, 381, 708, 810
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 5 cut(s) 171, 368, 408, 614, 1086
MspR9I CCNGG 1 cut(s) 615
MwoI GCNNNNNNNGC 5 cut(s) 359, 554, 857, 877, 956
NciI CCSGG 1 cut(s) 615
NdeII GATC 5 cut(s) 151, 715, 785, 1219, 1234
NlaIII CATG 7 cut(s) 246, 280, 498, 954, 994, 1081, 1217
NlaIV GGNNCC 3 cut(s) 757, 850, 1084
NmuCI GTSAC 2 cut(s) 245, 427
NspI RCATGY 2 cut(s) 246, 994
NspV TTCGAA 1 cut(s) 980
PagI TCATGA 1 cut(s) 1213
PciI ACATGT 2 cut(s) 242, 990
PciSI GCTCTTC 1 cut(s) 544
PfeI GAWTC 2 cut(s) 273, 1133
PfoI TCCNGGA 1 cut(s) 613
PinAI ACCGGT 1 cut(s) 170
PkrI GCNGC 3 cut(s) 606, 667, 1144
PleI GAGTC 1 cut(s) 1237
PpsI GAGTC 1 cut(s) 1237
PscI ACATGT 2 cut(s) 242, 990
PshBI ATTAAT 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 757, 850, 1084
PspPI GGNCC 2 cut(s) 640, 1083
RsaI GTAC 3 cut(s) 1070, 1166, 1249
RsaNI GTAC 3 cut(s) 1069, 1165, 1248
RseI CAYNNNNRTG 1 cut(s) 243
SapI GCTCTTC 1 cut(s) 544
SaqAI TTAA 4 cut(s) 66, 381, 708, 810
SatI GCNGC 3 cut(s) 605, 666, 1143
Sau3AI GATC 5 cut(s) 151, 715, 785, 1219, 1234
Sau96I GGNCC 2 cut(s) 640, 1083
SchI GAGTC 1 cut(s) 1237
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 4 cut(s) 124, 631, 697, 758
SfaNI GCATC 3 cut(s) 349, 813, 867
SfuI TTCGAA 1 cut(s) 980
SinI GGWCC 1 cut(s) 1083
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
Sse9I AATT 7 cut(s) 331, 459, 511, 621, 648, 907, 1093
SsiI CCGC 5 cut(s) 353, 602, 605, 846, 891
SspMI CTAG 3 cut(s) 210, 270, 783
StyD4I CCNGG 1 cut(s) 613
TaaI ACNGT 7 cut(s) 186, 227, 310, 433, 934, 970, 1054
TaqI TCGA 5 cut(s) 124, 162, 875, 946, 980
TasI AATT 7 cut(s) 331, 459, 511, 621, 648, 907, 1093
TauI GCSGC 1 cut(s) 607
TfiI GAWTC 2 cut(s) 273, 1133
Tru1I TTAA 4 cut(s) 66, 381, 708, 810
Tru9I TTAA 4 cut(s) 66, 381, 708, 810
TscAI CASTG 5 cut(s) 438, 511, 574, 642, 939
TseFI GTSAC 2 cut(s) 245, 427
TseI GCWGC 2 cut(s) 665, 1142
Tsp45I GTSAC 2 cut(s) 245, 427
TspDTI ATGAA 5 cut(s) 265, 736, 757, 1146, 1202
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 5 cut(s) 438, 511, 574, 642, 939
VneI GTGCAC 1 cut(s) 693
VpaK11BI GGWCC 1 cut(s) 1083
VspI ATTAAT 1 cut(s) 381
XapI RAATTY 2 cut(s) 331, 511
XbaI TCTAGA 1 cut(s) 269
XceI RCATGY 2 cut(s) 246, 994
XspI CTAG 3 cut(s) 210, 270, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.