FvH4_6g17730

Glucomannan 4-beta-mannosyltransferase 9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012740
Physical Location & Seq
Forward (+)
11621524 .. 11625920
4397 bp
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UTR
Exon/CDS
Intron
FvH4_6g17730.t1

Sequence Viewer

Length: 1602 bp
ATGGAGCGGCTTTCGTCGGCAGCGATTATTCCAGACTCGTTTATAGGCGCAAGAGATGATATTTTCATGCAATTTGGGTTGGTATGGAACATGATCAAGGAGCCGTTGATCATTCCTCTGCTGAGAATGGCAGTGGCTATGTGCTTGGTCATGTCGGTTATGATCTTCGCCGAGAGGCTTTACATGGGCGTCGTCATTATTCTGGTACATGTTTTCGGGCGAAAACCGGAGAAGCGATACAAATGGGAGCCCATCAAAGATGATGTTGAGTTGGGAAGCTCGAATTATCCCATGGTTCTGGTCCAAATCCCCATGTTCAATGAAAGAGAGGTTTATCAACTCTCAATCGGAGCTTGTTGTTCGCTAACATGGCCTTCTGATAGACTCATCATTCAAGTCCTTGATGATTCAACAGACCCAACTTGCAAGGCCATGGTAGAGCGAGAGTGCCAAAGATGGGCAGGCAAAGGCATAAACATCAAGTACGAAATCAGAGACAACAGAACAGGGTACAAATCAGGGGCTCTCAAAGAAGGCCTGAAGCGCAGCTACGCCAAAAACTGCGATTACGTCGCCATTTTCGACGCCGACTTTCAACCGGAGCCGGATTTCCTCTCTCGCAGCATTCCCTTCTTCGTCCACAATCCCAATGTCGCTCTTGTTCAGGCTCGCTGGAGATTCGTGAACAATAATGAGTGCTTGATGACAAGATTTCAAGAAATGTCACTGGACTATCATTTCAAAATTGAACAAGAAGTGGGCTCTTCCACACATGCATTCTTTGGGTTCAATGGGACTGCTGGTGTGTGGAGAATTGCTGCAATCAATGAGGCTGGAGGATGGAAGGATCGGACCACGGTGGAAGATATGGATCTAGCTGTTCGAGCAAGCCTCAAAGGGTGGAAATTTGTATATCTTGGTACTCTGCAGGTGAAGAGTGAATTGCCAAGTACTTTCAAGGCCTTCCGCCACCAGCAACATAGGTGGTCTTGTGGTCCGGCTAATCTATTCAAGAAAATGGTGATGGAAATTATAACAAACAAGAAAGTATCAGTATGGCAGAAAGTTCATGTGATCTACAGCTTCTTCTTCGTTAGGAAGATCATTGCACACATCTTTGCATTTTTCTTCTATTGCATTGTCTTGCCAGCAACTATTTTGGTGCCTGAAGTTACCGTACCAAAGTGGGGAGGAGTTTACATCCCTACCATCATTACCCTTCTTAATTCAGTTGGCACTCCAAGATCAATCCACTTGGTGGTCTTCTGGATCCTATTCGAGAACGTCATGTCCCTACACCGTACAAAAGCTACGATCATTGGTATACTAGAGTTTAGTCGAGTGAATGAATGGATCGTCACTGAAAAACTTGGAGATTTTCTTAAGAATAAAGTAGGAGCAAAAGCACCCAAAAAATTTCGATTGAATATTGGAGATAGGCTTCATTTCTTAGAGCTTGCTGTGGGATGTTATCTCTTCTTCTGTGCCTGTTATGATTTCGTCTTTGGCAAAGACAACTACTTCATCTTCTTTTATACCCAATCCCTTGCCTTCTTTATCATGGGATTTGGGTATGTTGGGACAATTGTTCCAACCTCTTAG

Protein Analysis

534

Amino Acids

61.32

Weight (kDa)

9.02

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 97 - 332 1.4e-15 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 102 - 263 8e-14 Glycosyl transferase family 2
Glyco_trans_2_3 PF13632 190 - 386 3.2e-22 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012740)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03760
fragaria_vesca FvH4_6g17730
malus_domestica MD12G1016200.v1.1 MD14G1014300.v1.1
prunus_persica Prupe.7G119100_v2.0.a1
pyrus_communis pycom12g01330 pycom14g01200
rosa_chinensis RchiOBHm_Chr3g0471961
rosa_laevigata RLG00000024116
rosa_multiflora Rmu_sc0000090.1_g000021
rosa_roxburghii Rroxscaffold_6G00409410
rosa_rugosa Rorug03G0121400
rosa_samantha Rh3AG172700 Rh3BG198200 Rh3CG189300 Rh3CG196700 Rh3DG176200
rosa_wichuraiana Rw3G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1034
AarI CACCTGC 1 cut(s) 919
Acc36I ACCTGC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 1162
AccB7I CCANNNNNTGG 1 cut(s) 1258
AccBSI CCGCTC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 1324
AciI CCGC 2 cut(s) 7, 967
AclWI GGATC 5 cut(s) 855, 879, 1264, 1277, 1361
AcsI RAATTY 2 cut(s) 905, 1415
AcuI CTGAAG 2 cut(s) 560, 1188
AcyI GRCGYC 2 cut(s) 189, 585
AdeI CACNNNGTG 1 cut(s) 1258
AfaI GTAC 7 cut(s) 207, 485, 512, 922, 952, 1179, 1303
AfiI CCNNNNNNNGG 3 cut(s) 457, 1187, 1258
AflII CTTAAG 1 cut(s) 1382
AflIII ACRYGT 1 cut(s) 208
AloI GAACNNNNNNTCC 2 cut(s) 1274, 1306
AluBI AGCT 7 cut(s) 279, 353, 549, 878, 1083, 1310, 1456
AluI AGCT 7 cut(s) 279, 353, 549, 878, 1083, 1310, 1456
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 5 cut(s) 855, 879, 1264, 1277, 1361
AoxI GGCC 4 cut(s) 371, 429, 535, 960
ApeKI GCWGC 4 cut(s) 20, 546, 621, 818
ApoI RAATTY 2 cut(s) 905, 1415
AspLEI GCGC 2 cut(s) 50, 546
AspS9I GGNCC 3 cut(s) 301, 852, 995
AsuHPI GGTGA 2 cut(s) 943, 1033
AvaII GGWCC 3 cut(s) 301, 852, 995
BamHI GGATCC 1 cut(s) 1269
BanI GGYRCC 1 cut(s) 1162
BanII GRGCYC 3 cut(s) 252, 526, 764
BarI GAAGNNNNNNTAC 4 cut(s) 533, 565, 1161, 1193
BbsI GAAGAC 1 cut(s) 1255
BbvI GCAGC 4 cut(s) 32, 558, 633, 805
BccI CCATC 5 cut(s) 260, 450, 834, 1018, 1217
BceAI ACGGC 1 cut(s) 88
BclI TGATCA 2 cut(s) 93, 108
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 2 cut(s) 875, 1328
BfmI CTRYAG 2 cut(s) 926, 1078
BfrI CTTAAG 1 cut(s) 1382
BfuAI ACCTGC 1 cut(s) 919
BisI GCNGC 5 cut(s) 8, 21, 547, 622, 819
BlsI GCNGC 5 cut(s) 9, 22, 548, 623, 820
BmcAI AGTACT 1 cut(s) 952
Bme18I GGWCC 3 cut(s) 301, 852, 995
BmgT120I GGNCC 3 cut(s) 301, 852, 995
BmiI GGNNCC 5 cut(s) 102, 249, 603, 1164, 1271
BpiI GAAGAC 1 cut(s) 1255
BplI GAGNNNNNCTC 2 cut(s) 876, 908
BpmI CTGGAG 2 cut(s) 694, 855
BsaBI GATNNNNATC 1 cut(s) 870
BsaHI GRCGYC 2 cut(s) 189, 585
BsaJI CCNNGG 3 cut(s) 291, 432, 855
BsaWI WCCGGW 2 cut(s) 226, 598
Bsc4I CCNNNNNNNGG 3 cut(s) 457, 1187, 1258
Bse1I ACTGG 1 cut(s) 732
Bse3DI GCAATG 1 cut(s) 1104
Bse8I GATNNNNATC 1 cut(s) 870
BseDI CCNNGG 3 cut(s) 291, 432, 855
BseGI GGATG 3 cut(s) 845, 1200, 1472
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 3 cut(s) 457, 1187, 1258
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 732
BseRI GAGGAG 1 cut(s) 1206
BseXI GCAGC 4 cut(s) 32, 558, 633, 805
BshFI GGCC 4 cut(s) 373, 431, 537, 962
BshNI GGYRCC 1 cut(s) 1162
BsiSI CCGG 4 cut(s) 227, 599, 605, 998
BslFI GGGAC 3 cut(s) 808, 1276, 1594
BslI CCNNNNNNNGG 3 cut(s) 457, 1187, 1258
BsmAI GTCTC 1 cut(s) 489
BsmFI GGGAC 3 cut(s) 808, 1276, 1594
BsmI GAATGC 2 cut(s) 624, 776
BsnI GGCC 4 cut(s) 373, 431, 537, 962
Bsp1286I GDGCHC 3 cut(s) 252, 526, 764
Bsp19I CCATGG 2 cut(s) 291, 432
BspACI CCGC 2 cut(s) 7, 967
BspANI GGCC 4 cut(s) 373, 431, 537, 962
BspCNI CTCAG 1 cut(s) 114
BspLI GGNNCC 5 cut(s) 102, 249, 603, 1164, 1271
BspMAI CTGCAG 1 cut(s) 930
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 5 cut(s) 855, 879, 1264, 1277, 1361
BspQI GCTCTTC 1 cut(s) 769
BspT107I GGYRCC 1 cut(s) 1162
BspTI CTTAAG 1 cut(s) 1382
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 1104
BsrI ACTGG 1 cut(s) 732
BssECI CCNNGG 3 cut(s) 291, 432, 855
BssNAI GTATAC 1 cut(s) 1325
BssNI GRCGYC 2 cut(s) 189, 585
BssT1I CCWWGG 2 cut(s) 291, 432
Bst1107I GTATAC 1 cut(s) 1325
Bst4CI ACNGT 3 cut(s) 859, 1177, 1301
Bst6I CTCTTC 3 cut(s) 769, 929, 1481
BstACI GRCGYC 2 cut(s) 189, 585
BstAFI CTTAAG 1 cut(s) 1382
BstC8I GCNNGC 5 cut(s) 463, 670, 889, 1149, 1458
BstDEI CTNAG 3 cut(s) 122, 1450, 1599
BstDSI CCRYGG 3 cut(s) 291, 432, 855
BstF5I GGATG 3 cut(s) 845, 1200, 1472
BstHHI GCGC 2 cut(s) 50, 546
BstMAI GTCTC 1 cut(s) 489
BstMWI GCNNNNNNNGC 3 cut(s) 370, 543, 884
BstNSI RCATGY 2 cut(s) 212, 776
BstSFI CTRYAG 2 cut(s) 926, 1078
BstV1I GCAGC 4 cut(s) 32, 558, 633, 805
BstV2I GAAGAC 1 cut(s) 1255
BstX2I RGATCY 2 cut(s) 871, 1269
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 2 cut(s) 871, 1269
BstZ17I GTATAC 1 cut(s) 1325
BsuRI GGCC 4 cut(s) 373, 431, 537, 962
BtgI CCRYGG 3 cut(s) 291, 432, 855
BtsCI GGATG 3 cut(s) 845, 1200, 1472
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 138, 725, 1359
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 5 cut(s) 463, 670, 889, 1149, 1458
CfoI GCGC 2 cut(s) 50, 546
Cfr13I GGNCC 3 cut(s) 301, 852, 995
CseI GACGC 2 cut(s) 178, 593
Csp6I GTAC 7 cut(s) 206, 484, 511, 921, 951, 1178, 1302
CspCI CAANNNNNGTGG 2 cut(s) 965, 1000
CviQI GTAC 7 cut(s) 206, 484, 511, 921, 951, 1178, 1302
DdeI CTNAG 3 cut(s) 122, 1450, 1599
DraIII CACNNNGTG 1 cut(s) 1258
Eam1104I CTCTTC 3 cut(s) 769, 929, 1481
EarI CTCTTC 3 cut(s) 769, 929, 1481
EciI GGCGGA 1 cut(s) 956
Eco130I CCWWGG 2 cut(s) 291, 432
Eco147I AGGCCT 2 cut(s) 537, 962
Eco24I GRGCYC 3 cut(s) 252, 526, 764
Eco47I GGWCC 3 cut(s) 301, 852, 995
Eco57I CTGAAG 2 cut(s) 560, 1188
EcoT14I CCWWGG 2 cut(s) 291, 432
EcoT22I ATGCAT 1 cut(s) 778
EcoT38I GRGCYC 3 cut(s) 252, 526, 764
ErhI CCWWGG 2 cut(s) 291, 432
FaqI GGGAC 3 cut(s) 808, 1276, 1594
FbaI TGATCA 2 cut(s) 93, 108
FblI GTMKAC 1 cut(s) 1324
Fnu4HI GCNGC 5 cut(s) 8, 21, 547, 622, 819
FokI GGATG 3 cut(s) 852, 1187, 1479
FriOI GRGCYC 3 cut(s) 252, 526, 764
Fsp4HI GCNGC 5 cut(s) 8, 21, 547, 622, 819
FspBI CTAG 2 cut(s) 875, 1328
GlaI GCGC 2 cut(s) 49, 545
GluI GCNGC 5 cut(s) 8, 21, 547, 622, 819
GsuI CTGGAG 2 cut(s) 694, 855
HaeIII GGCC 4 cut(s) 373, 431, 537, 962
HapII CCGG 4 cut(s) 227, 599, 605, 998
HgaI GACGC 2 cut(s) 178, 593
HhaI GCGC 2 cut(s) 50, 546
Hin1I GRCGYC 2 cut(s) 189, 585
Hin6I GCGC 2 cut(s) 48, 544
HinP1I GCGC 2 cut(s) 48, 544
HinfI GANTC 4 cut(s) 35, 384, 407, 678
HpaII CCGG 4 cut(s) 227, 599, 605, 998
HphI GGTGA 2 cut(s) 943, 1033
Hpy166II GTNNAC 4 cut(s) 640, 685, 1198, 1325
Hpy188I TCNGA 4 cut(s) 350, 379, 494, 852
Hpy188III TCNNGA 6 cut(s) 32, 682, 716, 1012, 1267, 1279
Hpy8I GTNNAC 4 cut(s) 640, 685, 1198, 1325
Hpy99I CGWCG 4 cut(s) 19, 194, 575, 587
HpyAV CCTTC 7 cut(s) 384, 527, 640, 838, 973, 1229, 1561
HpyCH4III ACNGT 3 cut(s) 859, 1177, 1301
HpyCH4IV ACGT 2 cut(s) 570, 1284
HpyCH4V TGCA 8 cut(s) 70, 426, 776, 821, 928, 1109, 1121, 1137
HpyF10VI GCNNNNNNNGC 3 cut(s) 370, 543, 884
HpyF3I CTNAG 3 cut(s) 122, 1450, 1599
HpySE526I ACGT 2 cut(s) 570, 1284
Hsp92I GRCGYC 2 cut(s) 189, 585
HspAI GCGC 2 cut(s) 48, 544
Ksp22I TGATCA 2 cut(s) 93, 108
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 6 cut(s) 4, 100, 247, 350, 601, 1397
Lsp1109I GCAGC 4 cut(s) 32, 558, 633, 805
MaeI CTAG 2 cut(s) 875, 1328
MaeII ACGT 2 cut(s) 570, 1284
MaeIII GTNAC 3 cut(s) 723, 1171, 1357
MbiI CCGCTC 1 cut(s) 7
MfeI CAATTG 1 cut(s) 1584
MflI RGATCY 2 cut(s) 871, 1269
MhlI GDGCHC 3 cut(s) 252, 526, 764
MlyI GAGTC 2 cut(s) 29, 378
MnlI CCTC 8 cut(s) 126, 168, 322, 623, 823, 830, 902, 1184
Mph1103I ATGCAT 1 cut(s) 778
MseI TTAA 2 cut(s) 1224, 1383
MspCI CTTAAG 1 cut(s) 1382
MspI CCGG 4 cut(s) 227, 599, 605, 998
MunI CAATTG 1 cut(s) 1584
Mva1269I GAATGC 2 cut(s) 624, 776
MwoI GCNNNNNNNGC 3 cut(s) 370, 543, 884
NcoI CCATGG 2 cut(s) 291, 432
NlaIV GGNNCC 5 cut(s) 102, 249, 603, 1164, 1271
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 2 cut(s) 723, 1357
NsiI ATGCAT 1 cut(s) 778
NspI RCATGY 2 cut(s) 212, 776
PaqCI CACCTGC 1 cut(s) 919
PceI AGGCCT 2 cut(s) 537, 962
PciI ACATGT 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 769
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 579
PctI GAATGC 2 cut(s) 624, 776
PfeI GAWTC 2 cut(s) 407, 678
PflMI CCANNNNNTGG 1 cut(s) 1258
PkrI GCNGC 5 cut(s) 9, 22, 548, 623, 820
PleI GAGTC 2 cut(s) 29, 378
PpsI GAGTC 2 cut(s) 29, 378
PscI ACATGT 1 cut(s) 208
PsiI TTATAA 1 cut(s) 1034
PspN4I GGNNCC 5 cut(s) 102, 249, 603, 1164, 1271
PspPI GGNCC 3 cut(s) 301, 852, 995
PstI CTGCAG 1 cut(s) 930
PsuI RGATCY 2 cut(s) 871, 1269
RsaI GTAC 7 cut(s) 207, 485, 512, 922, 952, 1179, 1303
RsaNI GTAC 7 cut(s) 206, 484, 511, 921, 951, 1178, 1302
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 2 cut(s) 1224, 1383
SatI GCNGC 5 cut(s) 8, 21, 547, 622, 819
Sau96I GGNCC 3 cut(s) 301, 852, 995
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 2 cut(s) 29, 378
SduI GDGCHC 3 cut(s) 252, 526, 764
SfcI CTRYAG 2 cut(s) 926, 1078
SinI GGWCC 3 cut(s) 301, 852, 995
SmlI CTYRAG 1 cut(s) 1382
SmoI CTYRAG 1 cut(s) 1382
SseBI AGGCCT 2 cut(s) 537, 962
SsiI CCGC 2 cut(s) 7, 967
SspI AATATT 1 cut(s) 1429
SspMI CTAG 2 cut(s) 875, 1328
StuI AGGCCT 2 cut(s) 537, 962
StyI CCWWGG 2 cut(s) 291, 432
TaaI ACNGT 3 cut(s) 859, 1177, 1301
TaiI ACGT 2 cut(s) 573, 1287
TaqI TCGA 6 cut(s) 281, 582, 883, 1278, 1339, 1420
TatI WGTACW 1 cut(s) 950
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 407, 678
Tru1I TTAA 2 cut(s) 1224, 1383
Tru9I TTAA 2 cut(s) 1224, 1383
TscAI CASTG 3 cut(s) 138, 732, 1366
TseFI GTSAC 2 cut(s) 723, 1357
TseI GCWGC 4 cut(s) 20, 546, 621, 818
Tsp45I GTSAC 2 cut(s) 723, 1357
TspDTI ATGAA 6 cut(s) 55, 336, 1058, 1362, 1433, 1513
TspRI CASTG 3 cut(s) 138, 732, 1366
Van91I CCANNNNNTGG 1 cut(s) 1258
Vha464I CTTAAG 1 cut(s) 1382
VpaK11BI GGWCC 3 cut(s) 301, 852, 995
XapI RAATTY 2 cut(s) 905, 1415
XceI RCATGY 2 cut(s) 212, 776
XmiI GTMKAC 1 cut(s) 1324
XspI CTAG 2 cut(s) 875, 1328
ZrmI AGTACT 1 cut(s) 952
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.