pycom12g01330

Glucomannan 4-beta-mannosyltransferase 9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012740
Physical Location & Seq
Forward (+)
1120137 .. 1124947
4811 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01330.8

Sequence Viewer

Length: 1608 bp
ATGGAGCGGCTGTCGACGGAGGCGAAGCTCCCGGATTCATTTTTTGGCGGCAGAGACGACATTTCCATGCAAATGGGGTTGATTTGGAGCCAGATCAATCCAGCCCCACTGATCGTTCCTTTGCTGCGGCTCGCCGTCGTTCTGTGTCTGATCATTTCGGTGATGCTCTTCGTCGAGCGGCTGTACATGGCGGTCGTCATCGTTCTGATCAAGCTCTTCGGGCGGAAGCCGGAGAGAAGGTACAAATGGGAGCCCATGAAAGATGACTTGGAGCTCGGAAACTCAGCTTATCCAATGGTTCTGGTCCAAATCCCAATGTACAATGAAAGAGAGGTTTATCAGCTTTCAATTGGAGCTGCATGCGGGCTTTCATGGCCATCCGATAGGATCATCATCCAAGTCCTTGATGATTCAACCGACCCAACCATCAAGGACATGGTGGAGTTGGAGTGCCAAAGGTGGGCAAGCAAGGGGATAAACATTAAGTACGAAATAAGAGACAACAGAAATGGCTACAAAGCTGGGGCTCTCAAAGAAGGCATGAAGCGCAGTTACGTCAAAAGCTGCGATTTTGTGGCCATTTTTGACGCGGATTTCCAGCCGGAGCCCGACTTTCTGTGGCGGACGATCCCCTTCCTCCTCCACAAGCCTGAGCTCGCACTCGTTCAGACTCGCTGGAAGTTCGTGAATTCAAACGAGTGCTTGATGACAAGAATGCAAGAAATGTCACTGGATTATCATTTTACTGTTGAACAAGAAGTGGGCTCCTCCACTCATGCCTTCTTTGGATTCAATGGGACTGCTGGTGTATGGAGAATTGCTGCAATCAATGAAGCCGGAGGATGGAAGGACCGGACGACAGTGGAAGATATGGATCTGGCTGTTCGAGCGAGTCTCAAGGGCTGGAAATTTGTATATCTTGGTACTATACAGGTGAAGAATGAATTGCCAAGCACTTTCAAAGCCTACCGCTACCAACAGCACAGGTGGTCTTGCGGTCCAGCTAATCTTTTCAGGAAAATGGTCATGGAAATTATAAGGAACAAGAAAGTAACATGCTGGAAGAAGGTACATGTGATTTACAGCTTCTTCTTCGTCAGGAAGATCATAGCGCATATCAACGCGTTTGTATTTTACTGCATTGTCTTGCCCGCAACTGTCCTGGTTCCTGAGGTTGTGGTGCCAAAGTGGGGAGGAATTTATATTCCTTCCTTGATTACCCTTCTCAATGCAGTTGGCACTCCAAGGTCAATCCACTTGTTGGTGTTCTGGATCCTATTTGAGAACGTCATGTCACTACACCGTACGAAGGCTACGATTATTGGTCTGCTAGAGGCTAGTCGAGTGAATGAATGGATCGTCACTGAGAAACTTGGAGATGCGCTCAAGACTTTGAATGTGGCCACAAAATTACCCCGAAAACCTAGATTCAGGATCGGAGAAAGGCTTCATTTGTTAGAGTTAGCTGTTGGATTGTACCTCTTCTTCTGTGGCTGTTACGACTTCATCTTTGGGAAGAACTACTTCTACCTGTTCCTTTACATCCAAGCAACTGCCTTCTTTATCATTGGTTTCGGGTATGTTGGGACATTCGTTCCCAACTCTTAA

Protein Analysis

536

Amino Acids

61.62

Weight (kDa)

9.13

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012740)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03760
fragaria_vesca FvH4_6g17730
malus_domestica MD12G1016200.v1.1 MD14G1014300.v1.1
prunus_persica Prupe.7G119100_v2.0.a1
pyrus_communis pycom12g01330 pycom14g01200
rosa_chinensis RchiOBHm_Chr3g0471961
rosa_laevigata RLG00000024116
rosa_multiflora Rmu_sc0000090.1_g000021
rosa_roxburghii Rroxscaffold_6G00409410
rosa_rugosa Rorug03G0121400
rosa_samantha Rh3AG172700 Rh3BG198200 Rh3CG189300 Rh3CG196700 Rh3DG176200
rosa_wichuraiana Rw3G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
AccB1I GGYRCC 1 cut(s) 1180
AccB7I CCANNNNNTGG 1 cut(s) 1261
AccBSI CCGCTC 2 cut(s) 7, 178
AccI GTMKAC 1 cut(s) 14
AccII CGCG 2 cut(s) 590, 1124
AclWI GGATC 7 cut(s) 395, 622, 882, 1267, 1280, 1364, 1442
AcoI YGGCCR 3 cut(s) 374, 576, 1401
AcsI RAATTY 3 cut(s) 688, 908, 1197
AfaI GTAC 8 cut(s) 185, 242, 320, 488, 925, 1071, 1306, 1478
AfiI CCNNNNNNNGG 5 cut(s) 460, 843, 1190, 1261, 1309
AflIII ACRYGT 2 cut(s) 1072, 1122
AgsI TTSAA 7 cut(s) 348, 414, 693, 752, 793, 961, 1396
AjnI CCWGG 1 cut(s) 1161
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
Alw21I GWGCWC 2 cut(s) 276, 657
Alw26I GTCTC 3 cut(s) 48, 492, 899
AlwI GGATC 7 cut(s) 395, 622, 882, 1267, 1280, 1364, 1442
AoxI GGCC 3 cut(s) 374, 576, 1401
ApeKI GCWGC 4 cut(s) 124, 356, 564, 821
ApoI RAATTY 3 cut(s) 688, 908, 1197
ArsI GACNNNNNNTTYG 2 cut(s) 1493, 1525
Asp700I GAANNNNTTC 2 cut(s) 1446, 1523
AspLEI GCGC 3 cut(s) 549, 1114, 1384
AspS9I GGNCC 3 cut(s) 304, 850, 998
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 172, 946
AvaII GGWCC 3 cut(s) 304, 850, 998
AxyI CCTNAGG 1 cut(s) 1170
BalI TGGCCA 3 cut(s) 376, 578, 1403
BamHI GGATCC 1 cut(s) 1272
BanI GGYRCC 1 cut(s) 1180
BanII GRGCYC 6 cut(s) 255, 276, 529, 609, 657, 767
BarI GAAGNNNNNNTAC 4 cut(s) 536, 568, 1469, 1501
Bbv12I GWGCWC 2 cut(s) 276, 657
BbvI GCAGC 4 cut(s) 111, 343, 551, 808
BccI CCATC 3 cut(s) 385, 434, 837
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 1163
BclI TGATCA 2 cut(s) 150, 207
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 3 cut(s) 48, 492, 899
BfaI CTAG 3 cut(s) 1331, 1338, 1425
BisI GCNGC 8 cut(s) 8, 49, 125, 128, 179, 357, 565, 822
BlsI GCNGC 8 cut(s) 9, 50, 126, 129, 180, 358, 566, 823
Bme1390I CCNGG 2 cut(s) 32, 1163
Bme18I GGWCC 3 cut(s) 304, 850, 998
BmgT120I GGNCC 3 cut(s) 304, 850, 998
BmiI GGNNCC 7 cut(s) 89, 252, 606, 766, 1167, 1182, 1274
BmrFI CCNGG 2 cut(s) 32, 1163
BmsI GCATC 2 cut(s) 153, 1369
BplI GAGNNNNNCTC 4 cut(s) 879, 911, 1368, 1400
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 2 cut(s) 881, 1370
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 2 cut(s) 392, 873
BsaJI CCNNGG 1 cut(s) 1244
BsaWI WCCGGW 1 cut(s) 852
BsaXI ACNNNNNCTCC 5 cut(s) 26, 224, 254, 434, 464
Bsc4I CCNNNNNNNGG 5 cut(s) 460, 843, 1190, 1261, 1309
Bse1I ACTGG 1 cut(s) 735
Bse21I CCTNAGG 1 cut(s) 1170
Bse8I GATNNNNATC 2 cut(s) 392, 873
BseBI CCWGG 1 cut(s) 1163
BseDI CCNNGG 1 cut(s) 1244
BseGI GGATG 4 cut(s) 377, 393, 848, 1542
BseJI GATNNNNATC 2 cut(s) 392, 873
BseLI CCNNNNNNNGG 5 cut(s) 460, 843, 1190, 1261, 1309
BseMII CTCAG 4 cut(s) 297, 642, 1161, 1356
BseNI ACTGG 1 cut(s) 735
BseRI GAGGAG 2 cut(s) 629, 757
BseXI GCAGC 4 cut(s) 111, 343, 551, 808
BseYI CCCAGC 1 cut(s) 521
Bsh1236I CGCG 2 cut(s) 590, 1124
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 3 cut(s) 376, 578, 1403
BshNI GGYRCC 1 cut(s) 1180
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 276, 657
BsiSI CCGG 5 cut(s) 32, 230, 602, 837, 853
BsiWI CGTACG 1 cut(s) 1304
BslFI GGGAC 2 cut(s) 811, 1600
BslI CCNNNNNNNGG 5 cut(s) 460, 843, 1190, 1261, 1309
BsmAI GTCTC 3 cut(s) 48, 492, 899
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 811, 1600
BsmI GAATGC 1 cut(s) 720
BsnI GGCC 3 cut(s) 376, 578, 1403
Bsp1286I GDGCHC 6 cut(s) 255, 276, 529, 609, 657, 767
Bsp1407I TGTACA 2 cut(s) 183, 318
BspANI GGCC 3 cut(s) 376, 578, 1403
BspCNI CTCAG 4 cut(s) 296, 643, 1162, 1357
BspFNI CGCG 2 cut(s) 590, 1124
BspLI GGNNCC 7 cut(s) 89, 252, 606, 766, 1167, 1182, 1274
BspPI GGATC 7 cut(s) 395, 622, 882, 1267, 1280, 1364, 1442
BspQI GCTCTTC 2 cut(s) 173, 221
BspT107I GGYRCC 1 cut(s) 1180
BsrBI CCGCTC 2 cut(s) 7, 178
BsrGI TGTACA 2 cut(s) 183, 318
BsrI ACTGG 1 cut(s) 735
BssECI CCNNGG 1 cut(s) 1244
BssT1I CCWWGG 1 cut(s) 1244
Bst2UI CCWGG 1 cut(s) 1163
Bst4CI ACNGT 4 cut(s) 748, 862, 1159, 1304
Bst6I CTCTTC 3 cut(s) 173, 221, 1487
BstAUI TGTACA 2 cut(s) 183, 318
BstC8I GCNNGC 6 cut(s) 132, 361, 365, 466, 657, 1152
BstDEI CTNAG 4 cut(s) 283, 651, 1170, 1365
BstF5I GGATG 4 cut(s) 377, 393, 848, 1542
BstFNI CGCG 2 cut(s) 590, 1124
BstHHI GCGC 3 cut(s) 549, 1114, 1384
BstMAI GTCTC 3 cut(s) 48, 492, 899
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 4 cut(s) 220, 373, 546, 887
BstNI CCWGG 1 cut(s) 1163
BstNSI RCATGY 3 cut(s) 363, 1059, 1076
BstSCI CCNGG 2 cut(s) 30, 1161
BstUI CGCG 2 cut(s) 590, 1124
BstV1I GCAGC 4 cut(s) 111, 343, 551, 808
BstX2I RGATCY 2 cut(s) 874, 1272
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 2 cut(s) 874, 1272
Bsu36I CCTNAGG 1 cut(s) 1170
BsuRI GGCC 3 cut(s) 376, 578, 1403
BtsCI GGATG 4 cut(s) 377, 393, 848, 1542
BtsIMutI CAGTG 4 cut(s) 107, 728, 867, 1362
Cac8I GCNNGC 6 cut(s) 132, 361, 365, 466, 657, 1152
CfoI GCGC 3 cut(s) 549, 1114, 1384
Cfr13I GGNCC 3 cut(s) 304, 850, 998
CseI GACGC 1 cut(s) 596
Csp6I GTAC 8 cut(s) 184, 241, 319, 487, 924, 1070, 1305, 1477
CviQI GTAC 8 cut(s) 184, 241, 319, 487, 924, 1070, 1305, 1477
DdeI CTNAG 4 cut(s) 283, 651, 1170, 1365
EaeI YGGCCR 3 cut(s) 374, 576, 1401
Eam1104I CTCTTC 3 cut(s) 173, 221, 1487
EarI CTCTTC 3 cut(s) 173, 221, 1487
EciI GGCGGA 2 cut(s) 238, 637
Ecl136II GAGCTC 2 cut(s) 274, 655
Eco130I CCWWGG 1 cut(s) 1244
Eco24I GRGCYC 6 cut(s) 255, 276, 529, 609, 657, 767
Eco47I GGWCC 3 cut(s) 304, 850, 998
Eco53kI GAGCTC 2 cut(s) 274, 655
Eco81I CCTNAGG 1 cut(s) 1170
EcoICRI GAGCTC 2 cut(s) 274, 655
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 1161
EcoT14I CCWWGG 1 cut(s) 1244
EcoT38I GRGCYC 6 cut(s) 255, 276, 529, 609, 657, 767
ErhI CCWWGG 1 cut(s) 1244
Esp3I CGTCTC 1 cut(s) 48
FalI AAGNNNNNCTT 2 cut(s) 1508, 1540
FaqI GGGAC 2 cut(s) 811, 1600
FauI CCCGC 2 cut(s) 356, 1159
FbaI TGATCA 2 cut(s) 150, 207
FblI GTMKAC 1 cut(s) 14
Fnu4HI GCNGC 8 cut(s) 8, 49, 125, 128, 179, 357, 565, 822
FokI GGATG 4 cut(s) 364, 380, 855, 1529
FriOI GRGCYC 6 cut(s) 255, 276, 529, 609, 657, 767
Fsp4HI GCNGC 8 cut(s) 8, 49, 125, 128, 179, 357, 565, 822
FspBI CTAG 3 cut(s) 1331, 1338, 1425
GlaI GCGC 3 cut(s) 548, 1113, 1383
GluI GCNGC 8 cut(s) 8, 49, 125, 128, 179, 357, 565, 822
GsaI CCCAGC 1 cut(s) 525
HaeIII GGCC 3 cut(s) 376, 578, 1403
HapII CCGG 5 cut(s) 32, 230, 602, 837, 853
HgaI GACGC 1 cut(s) 596
HhaI GCGC 3 cut(s) 549, 1114, 1384
Hin6I GCGC 3 cut(s) 547, 1112, 1382
HinP1I GCGC 3 cut(s) 547, 1112, 1382
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 6 cut(s) 35, 410, 670, 789, 892, 1428
HpaII CCGG 5 cut(s) 32, 230, 602, 837, 853
HphI GGTGA 2 cut(s) 172, 946
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 6 cut(s) 150, 207, 278, 382, 669, 1439
Hpy188III TCNNGA 7 cut(s) 685, 1015, 1099, 1169, 1270, 1387, 1432
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 3 cut(s) 19, 140, 176
HpyCH4III ACNGT 4 cut(s) 748, 862, 1159, 1304
HpyCH4IV ACGT 2 cut(s) 555, 1287
HpyCH4V TGCA 6 cut(s) 70, 359, 718, 824, 1140, 1232
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 373, 546, 887
HpyF3I CTNAG 4 cut(s) 283, 651, 1170, 1365
HpySE526I ACGT 2 cut(s) 555, 1287
HspAI GCGC 3 cut(s) 547, 1112, 1382
Ksp22I TGATCA 2 cut(s) 150, 207
LguI GCTCTTC 2 cut(s) 173, 221
LmnI GCTCC 8 cut(s) 4, 33, 87, 250, 271, 353, 604, 770
Lsp1109I GCAGC 4 cut(s) 111, 343, 551, 808
LweI GCATC 2 cut(s) 153, 1369
MaeI CTAG 3 cut(s) 1331, 1338, 1425
MaeII ACGT 2 cut(s) 555, 1287
MaeIII GTNAC 6 cut(s) 551, 726, 1051, 1293, 1360, 1496
MbiI CCGCTC 2 cut(s) 7, 178
MfeI CAATTG 1 cut(s) 348
MflI RGATCY 2 cut(s) 874, 1272
MhlI GDGCHC 6 cut(s) 255, 276, 529, 609, 657, 767
MlsI TGGCCA 3 cut(s) 376, 578, 1403
MluCI AATT 8 cut(s) 348, 688, 816, 908, 944, 1032, 1197, 1409
MluI ACGCGT 1 cut(s) 1122
MluNI TGGCCA 3 cut(s) 376, 578, 1403
MlyI GAGTC 2 cut(s) 664, 901
MmeI TCCRAC 2 cut(s) 426, 1450
Mox20I TGGCCA 3 cut(s) 376, 578, 1403
MroXI GAANNNNTTC 2 cut(s) 1446, 1523
MscI TGGCCA 3 cut(s) 376, 578, 1403
MseI TTAA 2 cut(s) 483, 1606
MslI CAYNNNNRTG 3 cut(s) 65, 71, 158
Msp20I TGGCCA 3 cut(s) 376, 578, 1403
MspI CCGG 5 cut(s) 32, 230, 602, 837, 853
MspR9I CCNGG 2 cut(s) 32, 1163
MunI CAATTG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 720
MvaI CCWGG 1 cut(s) 1163
MvnI CGCG 2 cut(s) 590, 1124
MwoI GCNNNNNNNGC 4 cut(s) 220, 373, 546, 887
NciI CCSGG 1 cut(s) 32
NlaIV GGNNCC 7 cut(s) 89, 252, 606, 766, 1167, 1182, 1274
NmuCI GTSAC 3 cut(s) 726, 1293, 1360
NspI RCATGY 3 cut(s) 363, 1059, 1076
PaeI GCATGC 1 cut(s) 363
PciI ACATGT 1 cut(s) 1072
PciSI GCTCTTC 2 cut(s) 173, 221
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 1313
PctI GAATGC 1 cut(s) 720
PdmI GAANNNNTTC 2 cut(s) 1446, 1523
PfeI GAWTC 4 cut(s) 35, 410, 789, 1428
Pfl23II CGTACG 1 cut(s) 1304
PflMI CCANNNNNTGG 1 cut(s) 1261
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 8 cut(s) 9, 50, 126, 129, 180, 358, 566, 823
PleI GAGTC 2 cut(s) 664, 900
PpsI GAGTC 2 cut(s) 664, 900
PscI ACATGT 1 cut(s) 1072
PsiI TTATAA 1 cut(s) 1037
Psp124BI GAGCTC 2 cut(s) 276, 657
Psp6I CCWGG 1 cut(s) 1161
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 1161
PspLI CGTACG 1 cut(s) 1304
PspN4I GGNNCC 7 cut(s) 89, 252, 606, 766, 1167, 1182, 1274
PspPI GGNCC 3 cut(s) 304, 850, 998
PsrI GAACNNNNNNTAC 2 cut(s) 1511, 1543
PsuI RGATCY 2 cut(s) 874, 1272
RsaI GTAC 8 cut(s) 185, 242, 320, 488, 925, 1071, 1306, 1478
RsaNI GTAC 8 cut(s) 184, 241, 319, 487, 924, 1070, 1305, 1477
RseI CAYNNNNRTG 3 cut(s) 65, 71, 158
SacI GAGCTC 2 cut(s) 276, 657
SalI GTCGAC 1 cut(s) 13
SapI GCTCTTC 2 cut(s) 173, 221
SaqAI TTAA 2 cut(s) 483, 1606
SatI GCNGC 8 cut(s) 8, 49, 125, 128, 179, 357, 565, 822
Sau96I GGNCC 3 cut(s) 304, 850, 998
SchI GAGTC 2 cut(s) 664, 901
ScrFI CCNGG 2 cut(s) 32, 1163
SduI GDGCHC 6 cut(s) 255, 276, 529, 609, 657, 767
SfaNI GCATC 2 cut(s) 153, 1369
SinI GGWCC 3 cut(s) 304, 850, 998
SmiMI CAYNNNNRTG 3 cut(s) 65, 71, 158
SmlI CTYRAG 2 cut(s) 896, 1385
SmoI CTYRAG 2 cut(s) 896, 1385
SphI GCATGC 1 cut(s) 363
Sse9I AATT 8 cut(s) 348, 688, 816, 908, 944, 1032, 1197, 1409
SspMI CTAG 3 cut(s) 1331, 1338, 1425
SstI GAGCTC 2 cut(s) 276, 657
StyD4I CCNGG 2 cut(s) 30, 1161
StyI CCWWGG 1 cut(s) 1244
TaaI ACNGT 4 cut(s) 748, 862, 1159, 1304
TaiI ACGT 2 cut(s) 558, 1290
TaqI TCGA 4 cut(s) 14, 174, 886, 1342
TasI AATT 8 cut(s) 348, 688, 816, 908, 944, 1032, 1197, 1409
TatI WGTACW 2 cut(s) 183, 318
TauI GCSGC 4 cut(s) 10, 51, 130, 181
TfiI GAWTC 4 cut(s) 35, 410, 789, 1428
Tru1I TTAA 2 cut(s) 483, 1606
Tru9I TTAA 2 cut(s) 483, 1606
TscAI CASTG 4 cut(s) 114, 735, 867, 1369
TseFI GTSAC 3 cut(s) 726, 1293, 1360
TseI GCWGC 4 cut(s) 124, 356, 564, 821
Tsp45I GTSAC 3 cut(s) 726, 1293, 1360
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 4 cut(s) 114, 735, 867, 1369
Van91I CCANNNNNTGG 1 cut(s) 1261
VpaK11BI GGWCC 3 cut(s) 304, 850, 998
XapI RAATTY 3 cut(s) 688, 908, 1197
XceI RCATGY 3 cut(s) 363, 1059, 1076
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 14
XmnI GAANNNNTTC 2 cut(s) 1446, 1523
XspI CTAG 3 cut(s) 1331, 1338, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.