Rorug03G0121400

Pentatricopeptide repeat-containing protein At5g12100

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000162
Physical Location & Seq
Forward (+)
9822435 .. 9826713
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0121400.1

Sequence Viewer

Length: 1431 bp
ATGGAAAACGGTCACCACCACACAGCCAGCGGCCCTCGCTCCGACGACCGTATCAAGCTCAACGTCGGAGGCAAGCTCTTCGAAACTACCTTATCGACAATCCAGGCAGGCGGTCCCGACTCTCTCTTAGCCGCCCTCTCGAGCCGGACCTCCGACGACCCGGACCCGGTTTTCATAGACCGGGACCCGGAGATCTTCTCAGTCATACTCTCTCTCCTCCGCTCCGACGGTCTCCCTTCCACGGCTCGCCGATTCGCCAAGCAAGAACTCGCCGATGAAGCTCTCTACTACGGCATCGAGTCGCAGCTCAAGTCGGCGATGTTGCCGCCTCCGTTATCCGGAATCGACGCCTCGATCGTCACAACTGTACATCCCGCCTCCGACGGCCTCCCCTCGGCGATCACAGCTGGTACCGACGGCTCCGTTTGGATCGCACACGGCGGTCAAATATCCAGCTACGATTGGAATCTGTGCCACGCCGGAACGATCCGTACTCATTTGGAGGACATAAACTCGATTTGCCGAGTGTATCCTGAAATCGCCGCCGTAGGATCCGAATCCGACCCGGGGCTCCACTTCTACGATTTCTCCGGCGTGCGAAACATGGGGGCGATCCACTGGACCGACCCGGCCGACCCGAGAATCTTCAAGGCCCGAGTTACGTCCGTCTCGAACTCGCCGAGCTCAGTCTTCGCCGCTTTCGATTGCCCGCACAGAGAGAACTGCATCTTAGTGATCGACAAATCAACGCTCAAGATCGTCGGTGAGCTGAGCAGGCAATCGGGAAGCTCGGCGAAGAACTTGGCCGTCGGAAAGCTGACGTGGCTGCCTGAAACGGGCTTGATTGTTGGGAGCTCCGTCACTTGCGGTGCGTTCGGGTACTCGGGTTATATCCGAATGTGGGACCCGAGGTCTAAGGAGGTGGTTTGGGAGACGAACGAGCCAGGGTCGGGGCGGAGCAGTAGGTTCGGGGACTCCTTCGCCGACATCGACGTGGACATTGAAGAGTCGACTCTGTTCAAGGTGTGTTCAAAGTCTGGAGATTTGGCAATGGCAGACCTGCGTAATTTAGGGGATGATCCGTGGGTTTATTTACAAGATAAGGACCCAAGCATGAGGAACATAAAGGGGGGTGGCAATAGCAGTAGTGTAATTCACTGTTACAAAAACCAAGCCTTTGTGGGCAGAGATGGTGCCTTGGAGGTTTGGTCGAGACTGCCGAAGAAAGCCGAAAATAGGGTGGATGGAGAAAGTGAGGTTTGCGAGGGATTGTATAGGAGGAATTTTGTGGATAAAGAGGAGGATGCTCAGAGAGGGATTATAAAGAAAATCGAAGGCGGTGGGAATAGGCTGTTTGTTGTTAGAGAAGATGTTGAAGGTATTGAGGTGTGGGAGAGCTCTCATTCTTCTGGTGCAGTTTCAGTTTTGTGA

Protein Analysis

476

Amino Acids

51.61

Weight (kDa)

5.15

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 18 - 102 5e-14 BTB/POZ domain
Beta_prop_At2g24240 PF25279 131 - 465 2.1e-18 At2g24240-like, C-terminal beta propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
Acc36I ACCTGC 1 cut(s) 1068
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 410, 1193
AccBSI CCGCTC 1 cut(s) 222
AccI GTMKAC 1 cut(s) 1010
AccIII TCCGGA 1 cut(s) 338
AclWI GGATC 6 cut(s) 437, 481, 546, 559, 607, 1073
AcoI YGGCCR 2 cut(s) 630, 804
AcsI RAATTY 1 cut(s) 1282
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 4 cut(s) 369, 412, 493, 881
AgsI TTSAA 5 cut(s) 649, 1004, 1021, 1032, 1376
AhdI GACNNNNNGTC 1 cut(s) 910
AjiI CACGTC 2 cut(s) 822, 994
AjnI CCWGG 2 cut(s) 102, 943
Alw21I GWGCWC 3 cut(s) 686, 857, 1400
Alw26I GTCTC 4 cut(s) 236, 673, 926, 1207
AlwI GGATC 6 cut(s) 437, 481, 546, 559, 607, 1073
Ama87I CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 5 cut(s) 31, 385, 630, 651, 804
ApeKI GCWGC 2 cut(s) 304, 826
ApoI RAATTY 1 cut(s) 1282
Asp718I GGTACC 1 cut(s) 410
AspS9I GGNCC 9 cut(s) 32, 113, 147, 163, 184, 621, 652, 904, 1105
AsuC2I CCSGG 7 cut(s) 161, 167, 182, 188, 566, 567, 629
AsuHPI GGTGA 2 cut(s) 5, 776
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
AvaII GGWCC 7 cut(s) 113, 147, 163, 184, 621, 904, 1105
BaeI ACNNNNGTAYC 4 cut(s) 34, 67, 871, 904
BamHI GGATCC 1 cut(s) 551
BanI GGYRCC 2 cut(s) 410, 1193
BanII GRGCYC 4 cut(s) 573, 686, 857, 1400
BbsI GAAGAC 1 cut(s) 682
Bbv12I GWGCWC 3 cut(s) 686, 857, 1400
BbvI GCAGC 2 cut(s) 316, 813
BccI CCATC 2 cut(s) 1184, 1238
BceAI ACGGC 7 cut(s) 258, 307, 400, 433, 454, 530, 791
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 949, 983
BciT130I CCWGG 2 cut(s) 104, 945
BciVI GTATCC 1 cut(s) 540
BcnI CCSGG 7 cut(s) 161, 167, 182, 188, 566, 567, 629
BcoDI GTCTC 4 cut(s) 236, 673, 926, 1207
BfuAI ACCTGC 1 cut(s) 1068
BfuI GTATCC 1 cut(s) 540
BglII AGATCT 1 cut(s) 192
BisI GCNGC 7 cut(s) 31, 132, 305, 326, 543, 696, 827
BlpI GCTNAGC 1 cut(s) 770
BlsI GCNGC 7 cut(s) 32, 133, 306, 327, 544, 697, 828
Bme1390I CCNGG 9 cut(s) 104, 161, 167, 182, 188, 566, 567, 629, 945
Bme18I GGWCC 7 cut(s) 113, 147, 163, 184, 621, 904, 1105
BmeRI GACNNNNNGTC 1 cut(s) 910
BmeT110I CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
BmgBI CACGTC 2 cut(s) 822, 994
BmgT120I GGNCC 9 cut(s) 32, 113, 147, 163, 184, 621, 652, 904, 1105
BmrFI CCNGG 9 cut(s) 104, 161, 167, 182, 188, 566, 567, 629, 945
BmsI GCATC 3 cut(s) 303, 735, 1294
BpiI GAAGAC 1 cut(s) 682
BplI GAGNNNNNCTC 4 cut(s) 60, 92, 182, 214
BpmI CTGGAG 1 cut(s) 1059
Bpu1102I GCTNAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 293, 737
BpuMI CCSGG 7 cut(s) 161, 167, 182, 188, 566, 567, 629
BsaBI GATNNNNATC 2 cut(s) 465, 556
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 8 cut(s) 240, 393, 565, 566, 908, 944, 1082, 1197
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 6 cut(s) 198, 228, 572, 602, 1193, 1223
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 1056
Bse8I GATNNNNATC 2 cut(s) 465, 556
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 2 cut(s) 104, 945
BseDI CCNNGG 8 cut(s) 240, 393, 565, 566, 908, 944, 1082, 1197
BseGI GGATG 4 cut(s) 370, 1081, 1249, 1309
BseJI GATNNNNATC 2 cut(s) 465, 556
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 4 cut(s) 213, 699, 761, 1322
BseNI ACTGG 1 cut(s) 623
BseRI GAGGAG 2 cut(s) 206, 1313
BseX3I CGGCCG 1 cut(s) 630
BseXI GCAGC 2 cut(s) 316, 813
Bsh1285I CGRYCG 3 cut(s) 49, 357, 633
BshFI GGCC 5 cut(s) 33, 387, 632, 653, 806
BshNI GGYRCC 2 cut(s) 410, 1193
BsiEI CGRYCG 3 cut(s) 49, 357, 633
BsiHKAI GWGCWC 3 cut(s) 686, 857, 1400
BsiHKCI CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
BslFI GGGAC 4 cut(s) 99, 197, 917, 986
BsmAI GTCTC 4 cut(s) 236, 673, 926, 1207
BsmBI CGTCTC 2 cut(s) 673, 926
BsmFI GGGAC 4 cut(s) 99, 197, 917, 986
BsnI GGCC 5 cut(s) 33, 387, 632, 653, 806
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 4 cut(s) 573, 686, 857, 1400
Bsp13I TCCGGA 1 cut(s) 338
Bsp1407I TGTACA 1 cut(s) 367
Bsp1720I GCTNAGC 1 cut(s) 770
BspANI GGCC 5 cut(s) 33, 387, 632, 653, 806
BspCNI CTCAG 4 cut(s) 212, 698, 762, 1321
BspEI TCCGGA 1 cut(s) 338
BspMI ACCTGC 1 cut(s) 1068
BspPI GGATC 6 cut(s) 437, 481, 546, 559, 607, 1073
BspQI GCTCTTC 1 cut(s) 83
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 410, 1193
BspTNI GGTCTC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 1056
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 8 cut(s) 240, 393, 565, 566, 908, 944, 1082, 1197
BssNI GRCGYC 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 1197
Bst2UI CCWGG 2 cut(s) 104, 945
Bst4CI ACNGT 5 cut(s) 11, 50, 230, 367, 1160
Bst6I CTCTTC 2 cut(s) 83, 999
BstACI GRCGYC 1 cut(s) 348
BstAUI TGTACA 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 7 cut(s) 28, 74, 109, 247, 596, 710, 776
BstDEI CTNAG 7 cut(s) 127, 199, 685, 730, 770, 915, 1308
BstDSI CCRYGG 2 cut(s) 240, 1082
BstEII GGTNACC 1 cut(s) 11
BstF5I GGATG 4 cut(s) 370, 1081, 1249, 1309
BstMAI GTCTC 4 cut(s) 236, 673, 926, 1207
BstMCI CGRYCG 3 cut(s) 49, 357, 633
BstMWI GCNNNNNNNGC 5 cut(s) 36, 278, 404, 775, 823
BstNI CCWGG 2 cut(s) 104, 945
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 9 cut(s) 102, 159, 165, 180, 186, 564, 565, 627, 943
BstV1I GCAGC 2 cut(s) 316, 813
BstV2I GAAGAC 1 cut(s) 682
BstX2I RGATCY 2 cut(s) 192, 551
BstYI RGATCY 2 cut(s) 192, 551
BstZI CGGCCG 1 cut(s) 630
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 5 cut(s) 33, 387, 632, 653, 806
BtgI CCRYGG 2 cut(s) 240, 1082
BtgZI GCGATG 1 cut(s) 332
BtrI CACGTC 2 cut(s) 822, 994
BtsCI GGATG 4 cut(s) 370, 1081, 1249, 1309
BtsIMutI CAGTG 2 cut(s) 616, 1156
BveI ACCTGC 1 cut(s) 1068
Cac8I GCNNGC 7 cut(s) 28, 74, 109, 247, 596, 710, 776
Cfr13I GGNCC 9 cut(s) 32, 113, 147, 163, 184, 621, 652, 904, 1105
Cfr9I CCCGGG 1 cut(s) 565
CseI GACGC 1 cut(s) 356
Csp6I GTAC 4 cut(s) 368, 411, 492, 880
CviAII CATG 2 cut(s) 604, 1114
CviQI GTAC 4 cut(s) 368, 411, 492, 880
DdeI CTNAG 7 cut(s) 127, 199, 685, 730, 770, 915, 1308
DriI GACNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 2 cut(s) 630, 804
EagI CGGCCG 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 83, 999
Eam1105I GACNNNNNGTC 1 cut(s) 910
EarI CTCTTC 2 cut(s) 83, 999
EciI GGCGGA 1 cut(s) 970
Ecl136II GAGCTC 3 cut(s) 684, 855, 1398
EclXI CGGCCG 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 1197
Eco24I GRGCYC 4 cut(s) 573, 686, 857, 1400
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 7 cut(s) 113, 147, 163, 184, 621, 904, 1105
Eco52I CGGCCG 1 cut(s) 630
Eco53kI GAGCTC 3 cut(s) 684, 855, 1398
Eco88I CYCGRG 6 cut(s) 139, 565, 637, 654, 883, 907
Eco91I GGTNACC 1 cut(s) 11
EcoICRI GAGCTC 3 cut(s) 684, 855, 1398
EcoO109I RGGNCCY 3 cut(s) 184, 904, 1105
EcoO65I GGTNACC 1 cut(s) 11
EcoRII CCWGG 2 cut(s) 102, 943
EcoT14I CCWWGG 1 cut(s) 1197
EcoT38I GRGCYC 4 cut(s) 573, 686, 857, 1400
ErhI CCWWGG 1 cut(s) 1197
Esp3I CGTCTC 2 cut(s) 673, 926
FaeI CATG 2 cut(s) 607, 1117
FaiI YATR 9 cut(s) 176, 206, 509, 605, 891, 1115, 1124, 1275, 1322
FaqI GGGAC 4 cut(s) 99, 197, 917, 986
FatI CATG 2 cut(s) 603, 1113
FauI CCCGC 2 cut(s) 382, 717
FblI GTMKAC 1 cut(s) 1010
Fnu4HI GCNGC 7 cut(s) 31, 132, 305, 326, 543, 696, 827
FokI GGATG 4 cut(s) 357, 1088, 1256, 1316
FriOI GRGCYC 4 cut(s) 573, 686, 857, 1400
Fsp4HI GCNGC 7 cut(s) 31, 132, 305, 326, 543, 696, 827
GluI GCNGC 7 cut(s) 31, 132, 305, 326, 543, 696, 827
GsuI CTGGAG 1 cut(s) 1059
HaeIII GGCC 5 cut(s) 33, 387, 632, 653, 806
HgaI GACGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 2 cut(s) 607, 1117
HincII GTYRAC 1 cut(s) 1011
HindII GTYRAC 1 cut(s) 1011
HphI GGTGA 2 cut(s) 5, 776
Hpy166II GTNNAC 2 cut(s) 997, 1011
Hpy188III TCNNGA 9 cut(s) 116, 139, 339, 533, 670, 754, 783, 1038, 1212
Hpy8I GTNNAC 2 cut(s) 997, 1011
HpyAV CCTTC 4 cut(s) 246, 988, 1328, 1370
HpyCH4III ACNGT 5 cut(s) 11, 50, 230, 367, 1160
HpyCH4IV ACGT 4 cut(s) 63, 662, 821, 993
HpyCH4V TGCA 2 cut(s) 726, 1415
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 278, 404, 775, 823
HpyF3I CTNAG 7 cut(s) 127, 199, 685, 730, 770, 915, 1308
HpySE526I ACGT 4 cut(s) 63, 662, 821, 993
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 2 cut(s) 607, 1117
KflI GGGWCCC 2 cut(s) 184, 904
Kpn2I TCCGGA 1 cut(s) 338
KpnI GGTACC 1 cut(s) 414
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 7 cut(s) 44, 227, 425, 576, 852, 860, 957
Lsp1109I GCAGC 2 cut(s) 316, 813
LweI GCATC 3 cut(s) 303, 735, 1294
MaeII ACGT 4 cut(s) 63, 662, 821, 993
MaeIII GTNAC 5 cut(s) 11, 358, 658, 859, 1160
MbiI CCGCTC 1 cut(s) 222
MboII GAAGA 9 cut(s) 70, 187, 637, 682, 808, 1016, 1234, 1379, 1398
MflI RGATCY 2 cut(s) 192, 551
MhlI GDGCHC 4 cut(s) 573, 686, 857, 1400
MluCI AATT 3 cut(s) 1066, 1152, 1282
MlyI GAGTC 5 cut(s) 113, 308, 968, 1006, 1016
MmeI TCCRAC 7 cut(s) 46, 66, 177, 249, 405, 585, 790
MroI TCCGGA 1 cut(s) 338
MslI CAYNNNNRTG 2 cut(s) 731, 992
MspA1I CMGCKG 2 cut(s) 30, 407
MspR9I CCNGG 9 cut(s) 104, 161, 167, 182, 188, 566, 567, 629, 945
MvaI CCWGG 2 cut(s) 104, 945
MwoI GCNNNNNNNGC 5 cut(s) 36, 278, 404, 775, 823
NciI CCSGG 7 cut(s) 161, 167, 182, 188, 566, 567, 629
NlaIII CATG 2 cut(s) 607, 1117
NmeAIII GCCGAG 4 cut(s) 374, 548, 705, 770
NmuCI GTSAC 3 cut(s) 11, 358, 859
NspV TTCGAA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 83
PcsI WCGNNNNNNNCGW 6 cut(s) 354, 668, 677, 699, 987, 1217
PfeI GAWTC 5 cut(s) 252, 342, 466, 557, 642
PkrI GCNGC 7 cut(s) 32, 133, 306, 327, 544, 697, 828
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 5 cut(s) 113, 307, 968, 1006, 1015
PpsI GAGTC 5 cut(s) 113, 307, 968, 1006, 1015
PpuMI RGGWCCY 3 cut(s) 184, 904, 1105
PsiI TTATAA 1 cut(s) 1322
Psp124BI GAGCTC 3 cut(s) 686, 857, 1400
Psp5II RGGWCCY 3 cut(s) 184, 904, 1105
Psp6I CCWGG 2 cut(s) 102, 943
PspEI GGTNACC 1 cut(s) 11
PspGI CCWGG 2 cut(s) 102, 943
PspPI GGNCC 9 cut(s) 32, 113, 147, 163, 184, 621, 652, 904, 1105
PspPPI RGGWCCY 3 cut(s) 184, 904, 1105
PsrI GAACNNNNNNTAC 2 cut(s) 475, 507
PsuI RGATCY 2 cut(s) 192, 551
PvuI CGATCG 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 4 cut(s) 369, 412, 493, 881
RsaNI GTAC 4 cut(s) 368, 411, 492, 880
RseI CAYNNNNRTG 2 cut(s) 731, 992
SacI GAGCTC 3 cut(s) 686, 857, 1400
SalI GTCGAC 1 cut(s) 1009
SapI GCTCTTC 1 cut(s) 83
SatI GCNGC 7 cut(s) 31, 132, 305, 326, 543, 696, 827
Sau96I GGNCC 9 cut(s) 32, 113, 147, 163, 184, 621, 652, 904, 1105
SchI GAGTC 5 cut(s) 113, 308, 968, 1006, 1016
ScrFI CCNGG 9 cut(s) 104, 161, 167, 182, 188, 566, 567, 629, 945
SduI GDGCHC 4 cut(s) 573, 686, 857, 1400
SfaNI GCATC 3 cut(s) 303, 735, 1294
Sfr274I CTCGAG 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 7 cut(s) 113, 147, 163, 184, 621, 904, 1105
SlaI CTCGAG 1 cut(s) 139
SmaI CCCGGG 1 cut(s) 567
SmiMI CAYNNNNRTG 2 cut(s) 731, 992
SmlI CTYRAG 3 cut(s) 139, 308, 752
SmoI CTYRAG 3 cut(s) 139, 308, 752
Sse9I AATT 3 cut(s) 1066, 1152, 1282
SstI GAGCTC 3 cut(s) 686, 857, 1400
StyD4I CCNGG 9 cut(s) 102, 159, 165, 180, 186, 564, 565, 627, 943
StyI CCWWGG 1 cut(s) 1197
TaaI ACNGT 5 cut(s) 11, 50, 230, 367, 1160
TaiI ACGT 4 cut(s) 66, 665, 824, 996
TaqII GACCGA 1 cut(s) 638
TasI AATT 3 cut(s) 1066, 1152, 1282
TatI WGTACW 1 cut(s) 367
TauI GCSGC 5 cut(s) 33, 134, 328, 545, 698
TfiI GAWTC 5 cut(s) 252, 342, 466, 557, 642
TscAI CASTG 2 cut(s) 623, 1163
TseFI GTSAC 3 cut(s) 11, 358, 859
TseI GCWGC 2 cut(s) 304, 826
Tsp45I GTSAC 3 cut(s) 11, 358, 859
TspDTI ATGAA 2 cut(s) 163, 291
TspGWI ACGGA 6 cut(s) 321, 412, 479, 655, 847, 1071
TspMI CCCGGG 1 cut(s) 565
TspRI CASTG 2 cut(s) 623, 1163
VpaK11BI GGWCC 7 cut(s) 113, 147, 163, 184, 621, 904, 1105
XapI RAATTY 1 cut(s) 1282
XhoI CTCGAG 1 cut(s) 139
XmaI CCCGGG 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.