Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 86 |
| AciI | CCGC | 5 cut(s) | 30, 90, 93, 200, 332 |
| AcoI | YGGCCR | 2 cut(s) | 165, 333 |
| AfaI | GTAC | 3 cut(s) | 206, 260, 354 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 83 |
| AgeI | ACCGGT | 1 cut(s) | 43 |
| AgsI | TTSAA | 1 cut(s) | 122 |
| AleI | CACNNNNGTG | 1 cut(s) | 341 |
| AluBI | AGCT | 3 cut(s) | 191, 241, 305 |
| AluI | AGCT | 3 cut(s) | 191, 241, 305 |
| Alw21I | GWGCWC | 2 cut(s) | 163, 295 |
| Alw26I | GTCTC | 2 cut(s) | 135, 292 |
| Alw44I | GTGCAC | 1 cut(s) | 291 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 150 |
| AoxI | GGCC | 2 cut(s) | 165, 333 |
| ApaLI | GTGCAC | 1 cut(s) | 291 |
| ApeKI | GCWGC | 1 cut(s) | 241 |
| AsiGI | ACCGGT | 1 cut(s) | 43 |
| AsuHPI | GGTGA | 1 cut(s) | 47 |
| BaeGI | GKGCMC | 1 cut(s) | 295 |
| BanI | GGYRCC | 1 cut(s) | 86 |
| Bbv12I | GWGCWC | 2 cut(s) | 163, 295 |
| BbvI | GCAGC | 1 cut(s) | 228 |
| BceAI | ACGGC | 1 cut(s) | 152 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 129, 163 |
| BcoDI | GTCTC | 2 cut(s) | 135, 292 |
| BfaI | CTAG | 1 cut(s) | 302 |
| BfmI | CTRYAG | 1 cut(s) | 96 |
| BisI | GCNGC | 4 cut(s) | 30, 93, 242, 333 |
| BlsI | GCNGC | 4 cut(s) | 31, 94, 243, 334 |
| BmiI | GGNNCC | 2 cut(s) | 48, 88 |
| BmsI | GCATC | 1 cut(s) | 358 |
| BsaAI | YACGTR | 1 cut(s) | 356 |
| BsaBI | GATNNNNATC | 1 cut(s) | 59 |
| BsaJI | CCNNGG | 1 cut(s) | 168 |
| BsaWI | WCCGGW | 1 cut(s) | 43 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 83 |
| Bse118I | RCCGGY | 4 cut(s) | 32, 43, 83, 244 |
| Bse1I | ACTGG | 1 cut(s) | 168 |
| Bse8I | GATNNNNATC | 1 cut(s) | 59 |
| BseDI | CCNNGG | 1 cut(s) | 168 |
| BseJI | GATNNNNATC | 1 cut(s) | 59 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BseMII | CTCAG | 1 cut(s) | 287 |
| BseNI | ACTGG | 1 cut(s) | 168 |
| BseSI | GKGCMC | 1 cut(s) | 295 |
| BseXI | GCAGC | 1 cut(s) | 228 |
| BsgI | GTGCAG | 1 cut(s) | 57 |
| Bsh1285I | CGRYCG | 1 cut(s) | 84 |
| BshFI | GGCC | 2 cut(s) | 167, 335 |
| BshNI | GGYRCC | 1 cut(s) | 86 |
| BshTI | ACCGGT | 1 cut(s) | 43 |
| BsiEI | CGRYCG | 1 cut(s) | 84 |
| BsiHKAI | GWGCWC | 2 cut(s) | 163, 295 |
| BsiSI | CCGG | 4 cut(s) | 33, 44, 84, 245 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 83 |
| BsmAI | GTCTC | 2 cut(s) | 135, 292 |
| BsnI | GGCC | 2 cut(s) | 167, 335 |
| Bsp1286I | GDGCHC | 2 cut(s) | 163, 295 |
| Bsp1407I | TGTACA | 1 cut(s) | 204 |
| Bsp143I | GATC | 1 cut(s) | 100 |
| BspACI | CCGC | 5 cut(s) | 30, 90, 93, 200, 332 |
| BspANI | GGCC | 2 cut(s) | 167, 335 |
| BspCNI | CTCAG | 1 cut(s) | 288 |
| BspLI | GGNNCC | 2 cut(s) | 48, 88 |
| BspT107I | GGYRCC | 1 cut(s) | 86 |
| BsrFI | RCCGGY | 4 cut(s) | 32, 43, 83, 244 |
| BsrGI | TGTACA | 1 cut(s) | 204 |
| BsrI | ACTGG | 1 cut(s) | 168 |
| BssAI | RCCGGY | 4 cut(s) | 32, 43, 83, 244 |
| BssECI | CCNNGG | 1 cut(s) | 168 |
| BssMI | GATC | 1 cut(s) | 100 |
| Bst4CI | ACNGT | 1 cut(s) | 263 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 377 |
| BstAUI | TGTACA | 1 cut(s) | 204 |
| BstBAI | YACGTR | 1 cut(s) | 356 |
| BstC8I | GCNNGC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 2 cut(s) | 237, 296 |
| BstDSI | CCRYGG | 1 cut(s) | 168 |
| BstKTI | GATC | 1 cut(s) | 103 |
| BstMAI | GTCTC | 2 cut(s) | 135, 292 |
| BstMBI | GATC | 1 cut(s) | 100 |
| BstMCI | CGRYCG | 1 cut(s) | 84 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 197, 377 |
| BstSFI | CTRYAG | 1 cut(s) | 96 |
| BstSLI | GKGCMC | 1 cut(s) | 295 |
| BstV1I | GCAGC | 1 cut(s) | 228 |
| BsuRI | GGCC | 2 cut(s) | 167, 335 |
| BtgI | CCRYGG | 1 cut(s) | 168 |
| BtsIMutI | CAGTG | 3 cut(s) | 50, 161, 293 |
| Cac8I | GCNNGC | 1 cut(s) | 246 |
| CaiI | CAGNNNCTG | 1 cut(s) | 150 |
| Cfr10I | RCCGGY | 4 cut(s) | 32, 43, 83, 244 |
| Csp6I | GTAC | 3 cut(s) | 205, 259, 353 |
| CspAI | ACCGGT | 1 cut(s) | 43 |
| CviAII | CATG | 2 cut(s) | 208, 377 |
| CviJI | RGCY | 7 cut(s) | 40, 167, 191, 241, 248, 305, 335 |
| CviKI_1 | RGCY | 7 cut(s) | 40, 167, 191, 241, 248, 305, 335 |
| CviQI | GTAC | 3 cut(s) | 205, 259, 353 |
| DdeI | CTNAG | 2 cut(s) | 237, 296 |
| DpnI | GATC | 1 cut(s) | 102 |
| DpnII | GATC | 1 cut(s) | 100 |
| EaeI | YGGCCR | 2 cut(s) | 165, 333 |
| FaeI | CATG | 2 cut(s) | 211, 380 |
| FaiI | YATR | 8 cut(s) | 155, 188, 209, 217, 219, 272, 315, 378 |
| FatI | CATG | 2 cut(s) | 207, 376 |
| Fnu4HI | GCNGC | 4 cut(s) | 30, 93, 242, 333 |
| Fsp4HI | GCNGC | 4 cut(s) | 30, 93, 242, 333 |
| FspBI | CTAG | 1 cut(s) | 302 |
| GluI | GCNGC | 4 cut(s) | 30, 93, 242, 333 |
| HaeIII | GGCC | 2 cut(s) | 167, 335 |
| HapII | CCGG | 4 cut(s) | 33, 44, 84, 245 |
| Hin1II | CATG | 2 cut(s) | 211, 380 |
| HpaII | CCGG | 4 cut(s) | 33, 44, 84, 245 |
| HphI | GGTGA | 1 cut(s) | 47 |
| Hpy166II | GTNNAC | 2 cut(s) | 205, 293 |
| Hpy188I | TCNGA | 1 cut(s) | 367 |
| Hpy188III | TCNNGA | 1 cut(s) | 140 |
| Hpy8I | GTNNAC | 2 cut(s) | 205, 293 |
| Hpy99I | CGWCG | 1 cut(s) | 68 |
| HpyCH4III | ACNGT | 1 cut(s) | 263 |
| HpyCH4IV | ACGT | 1 cut(s) | 355 |
| HpyCH4V | TGCA | 5 cut(s) | 74, 293, 346, 371, 380 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 197, 377 |
| HpyF3I | CTNAG | 2 cut(s) | 237, 296 |
| HpySE526I | ACGT | 1 cut(s) | 355 |
| Hsp92II | CATG | 2 cut(s) | 211, 380 |
| KroI | GCCGGC | 1 cut(s) | 244 |
| KroNI | GCCGGC | 1 cut(s) | 246 |
| Kzo9I | GATC | 1 cut(s) | 100 |
| LpnPI | CCDG | 8 cut(s) | 46, 57, 97, 130, 144, 149, 182, 258 |
| Lsp1109I | GCAGC | 1 cut(s) | 228 |
| LweI | GCATC | 1 cut(s) | 358 |
| MaeI | CTAG | 1 cut(s) | 302 |
| MaeII | ACGT | 1 cut(s) | 355 |
| MalI | GATC | 1 cut(s) | 102 |
| MboI | GATC | 1 cut(s) | 100 |
| MboII | GAAGA | 1 cut(s) | 301 |
| MhlI | GDGCHC | 2 cut(s) | 163, 295 |
| MluCI | AATT | 2 cut(s) | 123, 180 |
| MroNI | GCCGGC | 1 cut(s) | 244 |
| MseI | TTAA | 3 cut(s) | 126, 179, 228 |
| MslI | CAYNNNNRTG | 1 cut(s) | 341 |
| MspA1I | CMGCKG | 1 cut(s) | 332 |
| MspI | CCGG | 4 cut(s) | 33, 44, 84, 245 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 197, 377 |
| NaeI | GCCGGC | 1 cut(s) | 246 |
| NdeII | GATC | 1 cut(s) | 100 |
| NgoMIV | GCCGGC | 1 cut(s) | 244 |
| NlaIII | CATG | 2 cut(s) | 211, 380 |
| NlaIV | GGNNCC | 2 cut(s) | 48, 88 |
| OliI | CACNNNNGTG | 1 cut(s) | 341 |
| PdiI | GCCGGC | 1 cut(s) | 246 |
| PinAI | ACCGGT | 1 cut(s) | 43 |
| PkrI | GCNGC | 4 cut(s) | 31, 94, 243, 334 |
| Ppu21I | YACGTR | 1 cut(s) | 356 |
| PspN4I | GGNNCC | 2 cut(s) | 48, 88 |
| PstNI | CAGNNNCTG | 1 cut(s) | 150 |
| RsaI | GTAC | 3 cut(s) | 206, 260, 354 |
| RsaNI | GTAC | 3 cut(s) | 205, 259, 353 |
| RseI | CAYNNNNRTG | 1 cut(s) | 341 |
| SaqAI | TTAA | 3 cut(s) | 126, 179, 228 |
| SatI | GCNGC | 4 cut(s) | 30, 93, 242, 333 |
| Sau3AI | GATC | 1 cut(s) | 100 |
| SduI | GDGCHC | 2 cut(s) | 163, 295 |
| SetI | ASST | 7 cut(s) | 149, 193, 243, 307, 354, 358, 386 |
| SfaNI | GCATC | 1 cut(s) | 358 |
| SfcI | CTRYAG | 1 cut(s) | 96 |
| SgrAI | CRCCGGYG | 1 cut(s) | 32 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 341 |
| Sse9I | AATT | 2 cut(s) | 123, 180 |
| SsiI | CCGC | 5 cut(s) | 30, 90, 93, 200, 332 |
| SspMI | CTAG | 1 cut(s) | 302 |
| TaaI | ACNGT | 1 cut(s) | 263 |
| TaiI | ACGT | 1 cut(s) | 358 |
| TaqI | TCGA | 1 cut(s) | 139 |
| TasI | AATT | 2 cut(s) | 123, 180 |
| TatI | WGTACW | 1 cut(s) | 204 |
| TauI | GCSGC | 3 cut(s) | 32, 95, 335 |
| Tru1I | TTAA | 3 cut(s) | 126, 179, 228 |
| Tru9I | TTAA | 3 cut(s) | 126, 179, 228 |
| TscAI | CASTG | 3 cut(s) | 57, 168, 300 |
| TseI | GCWGC | 1 cut(s) | 241 |
| TspDTI | ATGAA | 2 cut(s) | 71, 330 |
| TspRI | CASTG | 3 cut(s) | 57, 168, 300 |
| VneI | GTGCAC | 1 cut(s) | 291 |
| XspI | CTAG | 1 cut(s) | 302 |