Rorug07G0084500

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000162
Physical Location & Seq
Reverse (-)
6657957 .. 6659983
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0084500.1

Sequence Viewer

Length: 729 bp
ATGAGCGATGACACGGAGAGCTCAAATTGCGAGCCTCCTGACCTTGAGAAGCAGCAGCACCGTGACGGCGGCGCTGAGGCGGTTACCTCAATTCCCGATTCTTCTGCCGTTGAGGATACGGTGCCGCCGCTGCTGAACGTCGTCGTTTCCGAGCCACCACCGGCTCCGACCGCCGCCACCGCCAATTCGTCCAAGGAGGTTAAGGGACAGCATTCCAGAAACTCCAGCGCTCATGAACAATGCAGGGTGTGTCAGCAGGATAAAGATGAAGCTTTGATTGATCTCGGATGCCATTGTCGAGGTGAACTTGCGAAAGCGCACAGATCATGTATAGACACTTGGTTTCAATCCAAAGGATCAAACAAATGCGAGATTTGCCAAGAGGTAGCTACGAATGTGTCAACTCCAGAATCCCAGCCAACTACAAATTATTGGGTTTGGAGAATTGAAACAACCTATCGATCGCAAAACCCAGACCAGGGCTGTATGAGCCCACTTTGGGTGGCATTCTCAATCCTCATTGGTGGTCTGCTGTTGGACGTGCTAATATCTGCCACCCTTGGCATCTCTGCAGTTCCCATTAACATTATCATCGGTGTTATTGTGGTCCTTGGGCTTGGAACTGTACTGAGACTTGCCTTGGCCTTTTGCCATGAGTGGGGTTCGAGGAGAGTTGTGCAGCGGGTGGACACACAGACAGTGCCTCTTGGCTACCATCCTGGTCTTTAG

Protein Analysis

242

Amino Acids

26.2

Weight (kDa)

5.1

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 81 - 126 6.3e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 8 cut(s) 69, 80, 125, 128, 171, 174, 180, 682
AclWI GGATC 1 cut(s) 364
AfaI GTAC 1 cut(s) 627
AfeI AGCGCT 1 cut(s) 229
AfiI CCNNNNNNNGG 4 cut(s) 478, 479, 499, 658
AgsI TTSAA 2 cut(s) 347, 449
AjiI CACGTC 1 cut(s) 541
AjnI CCWGG 2 cut(s) 477, 718
AluBI AGCT 3 cut(s) 21, 272, 389
AluI AGCT 3 cut(s) 21, 272, 389
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 625
AlwI GGATC 1 cut(s) 364
Aor51HI AGCGCT 1 cut(s) 229
AoxI GGCC 1 cut(s) 642
ApeKI GCWGC 4 cut(s) 52, 55, 130, 679
AspLEI GCGC 3 cut(s) 74, 230, 319
AspS9I GGNCC 1 cut(s) 607
AsuHPI GGTGA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 2 cut(s) 23, 494
Bbv12I GWGCWC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 4 cut(s) 64, 67, 117, 691
BccI CCATC 1 cut(s) 723
BceAI ACGGC 2 cut(s) 82, 92
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 479, 720
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 625
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 2 cut(s) 75, 231
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 680
BlsI GCNGC 8 cut(s) 54, 57, 71, 126, 129, 132, 175, 681
Bme1390I CCNGG 2 cut(s) 479, 720
Bme18I GGWCC 1 cut(s) 607
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 607
BmiI GGNNCC 2 cut(s) 123, 165
BmrFI CCNGG 2 cut(s) 479, 720
BmsI GCATC 2 cut(s) 278, 573
BpmI CTGGAG 2 cut(s) 208, 390
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 460
BsaJI CCNNGG 5 cut(s) 192, 478, 559, 610, 639
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 479, 499, 658
Bse118I RCCGGY 1 cut(s) 160
BseBI CCWGG 2 cut(s) 479, 720
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 5 cut(s) 192, 478, 559, 610, 639
BseGI GGATG 2 cut(s) 293, 715
BseLI CCNNNNNNNGG 4 cut(s) 478, 479, 499, 658
BseMII CTCAG 2 cut(s) 66, 620
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 4 cut(s) 64, 67, 117, 691
BseYI CCCAGC 1 cut(s) 414
BsgI GTGCAG 1 cut(s) 698
Bsh1285I CGRYCG 2 cut(s) 171, 464
BshFI GGCC 1 cut(s) 644
BshNI GGYRCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 460
BsiEI CGRYCG 2 cut(s) 171, 464
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 4 cut(s) 478, 479, 499, 658
BsmAI GTCTC 1 cut(s) 625
BsmFI GGGAC 1 cut(s) 219
BsmI GAATGC 2 cut(s) 211, 506
BsnI GGCC 1 cut(s) 644
Bsp1286I GDGCHC 2 cut(s) 23, 494
Bsp143I GATC 4 cut(s) 280, 323, 356, 461
BspACI CCGC 8 cut(s) 69, 80, 125, 128, 171, 174, 180, 682
BspANI GGCC 1 cut(s) 644
BspCNI CTCAG 2 cut(s) 67, 621
BspDI ATCGAT 1 cut(s) 460
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 123, 165
BspMAI CTGCAG 1 cut(s) 574
BspPI GGATC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 5 cut(s) 192, 478, 559, 610, 639
BssMI GATC 4 cut(s) 280, 323, 356, 461
BssT1I CCWWGG 4 cut(s) 192, 559, 610, 639
Bst2UI CCWGG 2 cut(s) 479, 720
Bst4CI ACNGT 4 cut(s) 62, 121, 625, 700
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 2 cut(s) 75, 629
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 2 cut(s) 293, 715
BstH2I RGCGCY 2 cut(s) 75, 231
BstHHI GCGC 3 cut(s) 74, 230, 319
BstKTI GATC 4 cut(s) 283, 326, 359, 464
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 4 cut(s) 280, 323, 356, 461
BstMCI CGRYCG 2 cut(s) 171, 464
BstMWI GCNNNNNNNGC 6 cut(s) 27, 130, 170, 179, 375, 489
BstNI CCWGG 2 cut(s) 479, 720
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 477, 718
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 64, 67, 117, 691
Bsu15I ATCGAT 1 cut(s) 460
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 644
BsuTUI ATCGAT 1 cut(s) 460
BtgZI GCGATG 1 cut(s) 21
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 2 cut(s) 293, 715
BtsIMutI CAGTG 1 cut(s) 705
Cac8I GCNNGC 1 cut(s) 32
CciI TCATGA 1 cut(s) 232
CfoI GCGC 3 cut(s) 74, 230, 319
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 607
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 626
CviAII CATG 3 cut(s) 233, 327, 653
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 2 cut(s) 75, 629
DpnI GATC 4 cut(s) 282, 325, 358, 463
DpnII GATC 4 cut(s) 280, 323, 356, 461
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 4 cut(s) 192, 559, 610, 639
Eco24I GRGCYC 2 cut(s) 23, 494
Eco47I GGWCC 1 cut(s) 607
Eco47III AGCGCT 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 21
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 477, 718
EcoT14I CCWWGG 4 cut(s) 192, 559, 610, 639
EcoT38I GRGCYC 2 cut(s) 23, 494
ErhI CCWWGG 4 cut(s) 192, 559, 610, 639
FaeI CATG 3 cut(s) 236, 330, 656
FaiI YATR 5 cut(s) 234, 328, 332, 488, 654
FaqI GGGAC 1 cut(s) 219
FatI CATG 3 cut(s) 232, 326, 652
FauI CCCGC 1 cut(s) 675
Fnu4HI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 680
FokI GGATG 2 cut(s) 300, 702
FriOI GRGCYC 2 cut(s) 23, 494
Fsp4HI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 680
GlaI GCGC 3 cut(s) 73, 229, 318
GluI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 680
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 2 cut(s) 208, 390
HaeII RGCGCY 2 cut(s) 75, 231
HaeIII GGCC 1 cut(s) 644
HapII CCGG 1 cut(s) 161
HhaI GCGC 3 cut(s) 74, 230, 319
Hin1II CATG 3 cut(s) 236, 330, 656
Hin6I GCGC 3 cut(s) 72, 228, 317
HinP1I GCGC 3 cut(s) 72, 228, 317
HincII GTYRAC 1 cut(s) 402
HindII GTYRAC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 2 cut(s) 98, 410
HpaII CCGG 1 cut(s) 161
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 3 cut(s) 305, 402, 688
Hpy188I TCNGA 3 cut(s) 151, 168, 287
Hpy188III TCNNGA 5 cut(s) 38, 95, 216, 233, 407
Hpy8I GTNNAC 3 cut(s) 305, 402, 688
Hpy99I CGWCG 2 cut(s) 143, 146
HpyCH4III ACNGT 4 cut(s) 62, 121, 625, 700
HpyCH4IV ACGT 2 cut(s) 138, 540
HpyCH4V TGCA 3 cut(s) 243, 572, 679
HpyF10VI GCNNNNNNNGC 6 cut(s) 27, 130, 170, 179, 375, 489
HpyF3I CTNAG 2 cut(s) 75, 629
HpySE526I ACGT 2 cut(s) 138, 540
Hsp92II CATG 3 cut(s) 236, 330, 656
HspAI GCGC 3 cut(s) 72, 228, 317
Kzo9I GATC 4 cut(s) 280, 323, 356, 461
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 4 cut(s) 64, 67, 117, 691
LweI GCATC 2 cut(s) 278, 573
MaeII ACGT 2 cut(s) 138, 540
MaeIII GTNAC 2 cut(s) 62, 82
MalI GATC 4 cut(s) 282, 325, 358, 463
MboI GATC 4 cut(s) 280, 323, 356, 461
MboII GAAGA 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 23, 494
MluCI AATT 5 cut(s) 25, 90, 184, 427, 444
MmeI TCCRAC 2 cut(s) 191, 516
MseI TTAA 2 cut(s) 201, 582
MspA1I CMGCKG 2 cut(s) 130, 682
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 479, 720
Mva1269I GAATGC 2 cut(s) 211, 506
MvaI CCWGG 2 cut(s) 479, 720
MwoI GCNNNNNNNGC 6 cut(s) 27, 130, 170, 179, 375, 489
NdeII GATC 4 cut(s) 280, 323, 356, 461
NlaIII CATG 3 cut(s) 236, 330, 656
NlaIV GGNNCC 2 cut(s) 123, 165
NmuCI GTSAC 1 cut(s) 62
PagI TCATGA 1 cut(s) 232
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 211, 506
PfeI GAWTC 2 cut(s) 98, 410
PkrI GCNGC 8 cut(s) 54, 57, 71, 126, 129, 132, 175, 681
Ple19I CGATCG 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 477, 718
PspEI GGTNACC 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 2 cut(s) 477, 718
PspN4I GGNNCC 2 cut(s) 123, 165
PspPI GGNCC 1 cut(s) 607
PstI CTGCAG 1 cut(s) 574
PvuI CGATCG 1 cut(s) 464
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 201, 582
SatI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 680
Sau3AI GATC 4 cut(s) 280, 323, 356, 461
Sau96I GGNCC 1 cut(s) 607
ScrFI CCNGG 2 cut(s) 479, 720
SduI GDGCHC 2 cut(s) 23, 494
SfaNI GCATC 2 cut(s) 278, 573
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 607
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 25, 90, 184, 427, 444
SsiI CCGC 8 cut(s) 69, 80, 125, 128, 171, 174, 180, 682
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 477, 718
StyI CCWWGG 4 cut(s) 192, 559, 610, 639
TaaI ACNGT 4 cut(s) 62, 121, 625, 700
TaiI ACGT 2 cut(s) 141, 543
TaqI TCGA 3 cut(s) 298, 460, 665
TasI AATT 5 cut(s) 25, 90, 184, 427, 444
TatI WGTACW 1 cut(s) 625
TauI GCSGC 4 cut(s) 72, 127, 130, 176
TfiI GAWTC 2 cut(s) 98, 410
Tru1I TTAA 2 cut(s) 201, 582
Tru9I TTAA 2 cut(s) 201, 582
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 4 cut(s) 52, 55, 130, 679
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 2 cut(s) 249, 282
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.