AT4G02570

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000162
Physical Location & Seq
Forward (+)
1128515 .. 1133774
5260 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02570.2

Sequence Viewer

Length: 2217 bp
ATGGAGCGCAAGACTATTGACTTGGAGCAAGGATGGGACTATATGCAGACTGGAATTACTAAGCTGAAACGGATTCTGGAAGGATTGAATGAGCCAGCATTTGACTCTGAGCAATATATGATGCTTTATACGACTATCTACAACATGTGCACCCAGAAACCTCCTCATGATTACTCACAGCAGCTTTATGACAAGTATCGGGAAGCATTTGAGGAATATATTAACTCAACAGTTTTGCCTGCGTTGAGGGAGAAGCATGATGAGTTTATGCTGCGGGAGCTATTTAAAAGATGGTCAAACCATAAAGTAATGGTCAGATGGCTATCCCGCTTCTTCTACTACCTTGACCGTTACTTCATTGCTCGGAGATCACTTCCACCACTGAATGAAGTTGGCCTGACATGCTTCCGTGACCTGGTTTATAATGAGCTACATTCTAAGGTCAAACAAGCTGTAATAGCTCTTGTTGATAAAGAACGGGAGGGCGAGCAGATTGATAGGGCCCTGCTGAAAAACGTATTAGATATCTATGTAGAGATTGGAATGGGGCAGATGGAGAGGTATGAAGAAGATTTTGAAAGCTTCATGCTTCAAGATACTTCTTCGTATTATTCTCGCAAGGCATCAAGCTGGATTCAGGAAGATTCTTGCCCTGATTACATGTTGAAGTCTGAAGAATGTCTAAAGAAGGAGAGGGAGAGAGTGGCTCACTACCTACACTCAAGCAGTGAGCCAAAGCTGGTTGAGAAAGTACAACATGAATTGCTGGTTGTGTTTGCAAGTCAGCTTCTAGAAAAAGAACACTCAGGGTGCCGTGCATTGCTAAGAGATGACAAGGTGGATGATCTCTCCAGGATGTACAGGCTTTACCATAAAATTTTGCGAGGCTTGGAACCTGTTGCAAACATCTTTAAGCAGCATGTCACAGCAGAGGGTAACGCTCTTGTCCAACAGGCCGAAGACACGGCTACTAATCAGGTTGCAAATACTGCTAGCGTCCAGGAACAGGTTCTTATCAGAAAAGTGATTGAACTTCATGATAAATACATGGTATATGTCACCGAGTGTTTCCAGAACCACACCCTCTTCCATAAGGCTTTGAAAGAGGCATTTGAGATTTTTTGTAACAAAACGGTTGCTGGAAGTTCAAGTGCAGAACTACTTGCAACATTTTGCGACAATATTCTCAAAAAGGGGGGAAGTGAAAAGCTGAGTGATGAAGCTATCGAAGATACGCTTGAGAAGGTTGTCAAATTGCTTGCATACATAAGTGACAAGGATCTTTTCGCTGAGTTCTACAGGAAGAAGCTGGCCCGTAGGCTCTTATTTGATCGCAGTGCTAATGATGATCATGAGAGAAGTATCCTGACAAAGCTCAAGCAACAATGTGGTGGACAGTTTACTTCTAAGATGGAGGGCATGGTGACGGATTTGACACTGGCAAGAGAAAACCAAAACAGTTTCGAGGATTATCTAGGCAGTAACCCTGCTGCAAACCCAGGGATTGACTTGACCGTCACTGTTCTTACCACTGGTTTCTGGCCAAGTTACAAATCATTTGACATAAATCTACCCAGTGAAATGATCAAGTGTGTTGAAGTCTTCAAAGGGTTTTATGAAACGAAAACGAAACACAGGAAGCTTACGTGGATCTATTCACTGGGAACTTGTCACATAAACGGGAAGTTTGATCAAAAGGCCATCGAGTTAATAGTGTCTACTTACCAGGCTGCTGTGCTTCTACTCTTTAACACAACTGACAAGTTAAGTTACACTGAGATCTTGGCTCAACTGAACCTAAGCCATGAAGATCTAGTTAGGTTGCTTCATTCCTTGTCATGTGCTAAGTACAAGATACTCCTTAAGGAGCCAAACACCAAGACTGTCTCCCAGAATGATGCCTTTGAGTTCAACTCCAAATTCACCGATAGAATGCGCAGAATCAAGATCCCTCTTCCCCCAGTTGATGAAAGGAAGAAAGTCGTTGAAGATGTCGATAAAGACAGAAGATATGCAATTGATGCTGCCATTGTCAGGATCATGAAGAGCAGGAAAGTATTGGGACATCAACAACTTGTTTCTGAGTGTGTTGAGCAACTTAGCCGAATGTTCAAGCCTGATATCAAAGCGATCAAGAAGCGTATGGAGGATTTAATAACCAGAGATTATTTGGAGAGGGACAAGGAGAATCCTAACATGTTTAGGTACTTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000151 GO:0000228 GO:0000793 GO:0000794 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009941 GO:0009987 GO:0010033 GO:0010087 GO:0010154 GO:0015630 GO:0019005 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042752 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0099402 GO:1901700 GO:1901701 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 ko05168 ko05200 map00190 map01100 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map04714 map04723 map04932 map05010 map05012 map05016 map05168 map05200
Pfam Domains
Protein Families
Cullin Cullin_Nedd8

Protein Analysis

738

Amino Acids

86.3

Weight (kDa)

6.71

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 31 - 483 4.2e-135 Cullin alpha solenoid domain
Cullin_AB PF26557 508 - 641 5.7e-45 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 668 - 730 8.9e-27 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AasI GACNNNNNNGTC 1 cut(s) 1514
Acc16I TGCGCA 1 cut(s) 1937
AccB1I GGYRCC 1 cut(s) 810
AccI GTMKAC 1 cut(s) 1718
AciI CCGC 2 cut(s) 274, 328
AclWI GGATC 4 cut(s) 1287, 1658, 1942, 2045
AcoI YGGCCR 1 cut(s) 1541
AcsI RAATTY 2 cut(s) 876, 1919
AcuI CTGAAG 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 1065
AfaI GTAC 4 cut(s) 753, 860, 1850, 2207
AfiI CCNNNNNNNGG 3 cut(s) 415, 1006, 2034
AflII CTTAAG 1 cut(s) 1862
AflIII ACRYGT 3 cut(s) 144, 660, 2196
AjnI CCWGG 5 cut(s) 414, 851, 999, 1498, 1725
Alw21I GWGCWC 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 1891
Alw44I GTGCAC 1 cut(s) 148
AlwI GGATC 4 cut(s) 1287, 1658, 1942, 2045
AoxI GGCC 6 cut(s) 394, 501, 954, 1311, 1541, 1698
ApaI GGGCCC 1 cut(s) 505
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 6 cut(s) 181, 271, 916, 1490, 1730, 2024
ApoI RAATTY 2 cut(s) 876, 1919
Asp700I GAANNNNTTC 1 cut(s) 1008
AspLEI GCGC 2 cut(s) 9, 1938
AspS9I GGNCC 3 cut(s) 501, 502, 1312
AsuHPI GGTGA 3 cut(s) 1051, 1435, 1915
AsuNHI GCTAGC 1 cut(s) 992
BaeGI GKGCMC 2 cut(s) 152, 505
BalI TGGCCA 1 cut(s) 1543
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 505
BbsI GAAGAC 2 cut(s) 966, 1594
Bbv12I GWGCWC 1 cut(s) 152
BbvI GCAGC 6 cut(s) 193, 258, 928, 1477, 1717, 2011
BccI CCATC 6 cut(s) 27, 285, 312, 547, 1405, 1709
BceAI ACGGC 2 cut(s) 798, 981
BciT130I CCWGG 5 cut(s) 416, 853, 1001, 1500, 1727
BciVI GTATCC 1 cut(s) 1373
BclI TGATCA 3 cut(s) 1348, 1584, 1690
BcoDI GTCTC 1 cut(s) 1891
BfaI CTAG 4 cut(s) 791, 993, 1475, 1814
BfmI CTRYAG 1 cut(s) 1297
BfrI CTTAAG 1 cut(s) 1862
BfuI GTATCC 1 cut(s) 1373
BglII AGATCT 2 cut(s) 1779, 1810
BisI GCNGC 6 cut(s) 182, 272, 917, 1491, 1731, 2025
BlsI GCNGC 6 cut(s) 183, 273, 918, 1492, 1732, 2026
Bme1390I CCNGG 5 cut(s) 416, 853, 1001, 1500, 1727
BmgT120I GGNCC 3 cut(s) 501, 502, 1312
BmiI GGNNCC 4 cut(s) 503, 812, 894, 1869
BmrFI CCNGG 5 cut(s) 416, 853, 1001, 1500, 1727
BmrI ACTGGG 3 cut(s) 1569, 1670, 1955
BmsI GCATC 4 cut(s) 111, 632, 1888, 2011
BmtI GCTAGC 1 cut(s) 996
BmuI ACTGGG 3 cut(s) 1569, 1670, 1955
BpiI GAAGAC 2 cut(s) 966, 1594
BplI GAGNNNNNCTC 4 cut(s) 691, 723, 1898, 1930
BpmI CTGGAG 1 cut(s) 835
Bpu10I CCTNAGC 1 cut(s) 1799
BpuEI CTTGAG 3 cut(s) 706, 1259, 1361
BsaAI YACGTR 1 cut(s) 1647
BsaBI GATNNNNATC 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 1498, 1499
Bsc4I CCNNNNNNNGG 3 cut(s) 415, 1006, 2034
Bse1I ACTGG 6 cut(s) 55, 1443, 1537, 1575, 1665, 1961
Bse3DI GCAATG 2 cut(s) 357, 818
Bse8I GATNNNNATC 1 cut(s) 322
BseBI CCWGG 5 cut(s) 416, 853, 1001, 1500, 1727
BseDI CCNNGG 2 cut(s) 1498, 1499
BseGI GGATG 3 cut(s) 38, 847, 861
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 3 cut(s) 415, 1006, 2034
BseMI GCAATG 2 cut(s) 357, 818
BseMII CTCAG 6 cut(s) 99, 819, 1202, 1281, 1767, 2073
BseNI ACTGG 6 cut(s) 55, 1443, 1537, 1575, 1665, 1961
BseRI GAGGAG 1 cut(s) 153
BseSI GKGCMC 2 cut(s) 152, 505
BseXI GCAGC 6 cut(s) 193, 258, 928, 1477, 1717, 2011
BsgI GTGCAG 1 cut(s) 1173
BshFI GGCC 6 cut(s) 396, 503, 956, 1313, 1543, 1700
BshNI GGYRCC 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 152
BslFI GGGAC 3 cut(s) 50, 2076, 2192
BslI CCNNNNNNNGG 3 cut(s) 415, 1006, 2034
BsmAI GTCTC 1 cut(s) 1891
BsmFI GGGAC 3 cut(s) 50, 2076, 2192
BsmI GAATGC 1 cut(s) 1938
BsnI GGCC 6 cut(s) 396, 503, 956, 1313, 1543, 1700
Bsp120I GGGCCC 1 cut(s) 501
Bsp1286I GDGCHC 2 cut(s) 152, 505
Bsp1407I TGTACA 1 cut(s) 858
BspACI CCGC 2 cut(s) 274, 328
BspANI GGCC 6 cut(s) 396, 503, 956, 1313, 1543, 1700
BspCNI CTCAG 6 cut(s) 100, 818, 1203, 1282, 1768, 2074
BspHI TCATGA 4 cut(s) 166, 1036, 1351, 2040
BspLI GGNNCC 4 cut(s) 503, 812, 894, 1869
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 4 cut(s) 1287, 1658, 1942, 2045
BspQI GCTCTTC 1 cut(s) 2039
BspT107I GGYRCC 1 cut(s) 810
BspTI CTTAAG 1 cut(s) 1862
BsrDI GCAATG 2 cut(s) 357, 818
BsrGI TGTACA 1 cut(s) 858
BsrI ACTGG 6 cut(s) 55, 1443, 1537, 1575, 1665, 1961
BssECI CCNNGG 2 cut(s) 1498, 1499
Bst2UI CCWGG 5 cut(s) 416, 853, 1001, 1500, 1727
Bst4CI ACNGT 8 cut(s) 232, 350, 1135, 1398, 1460, 1516, 1522, 1885
Bst6I CTCTTC 3 cut(s) 1091, 1959, 2039
BstAFI CTTAAG 1 cut(s) 1862
BstAPI GCANNNNNTGC 2 cut(s) 989, 2021
BstAUI TGTACA 1 cut(s) 858
BstBAI YACGTR 1 cut(s) 1647
BstC8I GCNNGC 6 cut(s) 96, 240, 488, 994, 1260, 1311
BstF5I GGATG 3 cut(s) 38, 847, 861
BstHHI GCGC 2 cut(s) 9, 1938
BstMAI GTCTC 1 cut(s) 1891
BstMWI GCNNNNNNNGC 5 cut(s) 277, 402, 458, 989, 2021
BstNI CCWGG 5 cut(s) 416, 853, 1001, 1500, 1727
BstNSI RCATGY 5 cut(s) 148, 405, 664, 923, 2200
BstSCI CCNGG 5 cut(s) 414, 851, 999, 1498, 1725
BstSFI CTRYAG 1 cut(s) 1297
BstSLI GKGCMC 2 cut(s) 152, 505
BstV1I GCAGC 6 cut(s) 193, 258, 928, 1477, 1717, 2011
BstV2I GAAGAC 2 cut(s) 966, 1594
BstX2I RGATCY 5 cut(s) 1279, 1650, 1779, 1810, 1947
BstYI RGATCY 5 cut(s) 1279, 1650, 1779, 1810, 1947
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 6 cut(s) 396, 503, 956, 1313, 1543, 1700
BtsCI GGATG 3 cut(s) 38, 847, 861
BtsI GCAGTG 2 cut(s) 733, 1342
BtsIMutI CAGTG 9 cut(s) 380, 733, 1342, 1436, 1518, 1530, 1582, 1658, 1773
Cac8I GCNNGC 6 cut(s) 96, 240, 488, 994, 1260, 1311
CciI TCATGA 4 cut(s) 166, 1036, 1351, 2040
CfoI GCGC 2 cut(s) 9, 1938
Cfr13I GGNCC 3 cut(s) 501, 502, 1312
CseI GACGC 1 cut(s) 985
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 4 cut(s) 752, 859, 1849, 2206
CviQI GTAC 4 cut(s) 752, 859, 1849, 2206
DraI TTTAAA 1 cut(s) 286
DraIII CACNNNGTG 1 cut(s) 1065
DrdI GACNNNNNNGTC 1 cut(s) 1514
DseDI GACNNNNNNGTC 1 cut(s) 1514
EaeI YGGCCR 1 cut(s) 1541
Eam1104I CTCTTC 3 cut(s) 1091, 1959, 2039
EarI CTCTTC 3 cut(s) 1091, 1959, 2039
Eco24I GRGCYC 1 cut(s) 505
Eco32I GATATC 2 cut(s) 526, 2122
Eco57I CTGAAG 1 cut(s) 693
EcoO109I RGGNCCY 2 cut(s) 501, 502
EcoRII CCWGG 5 cut(s) 414, 851, 999, 1498, 1725
EcoRV GATATC 2 cut(s) 526, 2122
EcoT38I GRGCYC 1 cut(s) 505
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 3 cut(s) 50, 2076, 2192
FauI CCCGC 2 cut(s) 267, 335
FbaI TGATCA 3 cut(s) 1348, 1584, 1690
FblI GTMKAC 1 cut(s) 1718
Fnu4HI GCNGC 6 cut(s) 182, 272, 917, 1491, 1731, 2025
FokI GGATG 3 cut(s) 45, 854, 868
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 6 cut(s) 182, 272, 917, 1491, 1731, 2025
FspBI CTAG 4 cut(s) 791, 993, 1475, 1814
FspI TGCGCA 1 cut(s) 1937
GlaI GCGC 2 cut(s) 8, 1937
GluI GCNGC 6 cut(s) 182, 272, 917, 1491, 1731, 2025
GsuI CTGGAG 1 cut(s) 835
HaeIII GGCC 6 cut(s) 396, 503, 956, 1313, 1543, 1700
HgaI GACGC 1 cut(s) 985
HhaI GCGC 2 cut(s) 9, 1938
Hin6I GCGC 2 cut(s) 7, 1936
HinP1I GCGC 2 cut(s) 7, 1936
HindIII AAGCTT 2 cut(s) 580, 1640
HinfI GANTC 6 cut(s) 73, 104, 634, 644, 1941, 2188
HphI GGTGA 3 cut(s) 1051, 1435, 1915
Hpy166II GTNNAC 4 cut(s) 150, 1394, 1401, 1719
Hpy188I TCNGA 6 cut(s) 109, 317, 366, 673, 1019, 2083
Hpy8I GTNNAC 4 cut(s) 150, 1394, 1401, 1719
HpyAV CCTTC 3 cut(s) 74, 682, 1237
HpyCH4III ACNGT 8 cut(s) 232, 350, 1135, 1398, 1460, 1516, 1522, 1885
HpyCH4IV ACGT 2 cut(s) 516, 1646
HpyF10VI GCNNNNNNNGC 5 cut(s) 277, 402, 458, 989, 2021
HpySE526I ACGT 2 cut(s) 516, 1646
HspAI GCGC 2 cut(s) 7, 1936
Ksp22I TGATCA 3 cut(s) 1348, 1584, 1690
LguI GCTCTTC 1 cut(s) 2039
LmnI GCTCC 4 cut(s) 4, 25, 277, 1867
Lsp1109I GCAGC 6 cut(s) 193, 258, 928, 1477, 1717, 2011
LweI GCATC 4 cut(s) 111, 632, 1888, 2011
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 4 cut(s) 791, 993, 1475, 1814
MaeII ACGT 2 cut(s) 516, 1646
MfeI CAATTG 1 cut(s) 2016
MflI RGATCY 5 cut(s) 1279, 1650, 1779, 1810, 1947
MhlI GDGCHC 2 cut(s) 152, 505
MlsI TGGCCA 1 cut(s) 1543
MluCI AATT 6 cut(s) 54, 761, 876, 1253, 1919, 2016
MluNI TGGCCA 1 cut(s) 1543
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 973
Mox20I TGGCCA 1 cut(s) 1543
MroXI GAANNNNTTC 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 1543
MseI TTAA 8 cut(s) 222, 285, 912, 1709, 1749, 1766, 1863, 2153
Msp20I TGGCCA 1 cut(s) 1543
MspCI CTTAAG 1 cut(s) 1862
MspR9I CCNGG 5 cut(s) 416, 853, 1001, 1500, 1727
MunI CAATTG 1 cut(s) 2016
Mva1269I GAATGC 1 cut(s) 1938
MvaI CCWGG 5 cut(s) 416, 853, 1001, 1500, 1727
MwoI GCNNNNNNNGC 5 cut(s) 277, 402, 458, 989, 2021
NheI GCTAGC 1 cut(s) 992
NlaIV GGNNCC 4 cut(s) 503, 812, 894, 1869
NmuCI GTSAC 7 cut(s) 410, 922, 1057, 1271, 1423, 1516, 1670
NsbI TGCGCA 1 cut(s) 1937
NspI RCATGY 5 cut(s) 148, 405, 664, 923, 2200
PagI TCATGA 4 cut(s) 166, 1036, 1351, 2040
PasI CCCWGGG 1 cut(s) 1499
PciI ACATGT 3 cut(s) 144, 660, 2196
PciSI GCTCTTC 1 cut(s) 2039
PctI GAATGC 1 cut(s) 1938
PdmI GAANNNNTTC 1 cut(s) 1008
PfeI GAWTC 5 cut(s) 73, 634, 644, 1941, 2188
PfoI TCCNGGA 2 cut(s) 851, 999
PkrI GCNGC 6 cut(s) 183, 273, 918, 1492, 1732, 2026
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 1647
PscI ACATGT 3 cut(s) 144, 660, 2196
PsiI TTATAA 1 cut(s) 423
Psp6I CCWGG 5 cut(s) 414, 851, 999, 1498, 1725
PspGI CCWGG 5 cut(s) 414, 851, 999, 1498, 1725
PspN4I GGNNCC 4 cut(s) 503, 812, 894, 1869
PspOMI GGGCCC 1 cut(s) 501
PspPI GGNCC 3 cut(s) 501, 502, 1312
PsuI RGATCY 5 cut(s) 1279, 1650, 1779, 1810, 1947
RsaI GTAC 4 cut(s) 753, 860, 1850, 2207
RsaNI GTAC 4 cut(s) 752, 859, 1849, 2206
SapI GCTCTTC 1 cut(s) 2039
SaqAI TTAA 8 cut(s) 222, 285, 912, 1709, 1749, 1766, 1863, 2153
SatI GCNGC 6 cut(s) 182, 272, 917, 1491, 1731, 2025
Sau96I GGNCC 3 cut(s) 501, 502, 1312
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 5 cut(s) 416, 853, 1001, 1500, 1727
SduI GDGCHC 2 cut(s) 152, 505
SexAI ACCWGGT 1 cut(s) 414
SfaNI GCATC 4 cut(s) 111, 632, 1888, 2011
SfcI CTRYAG 1 cut(s) 1297
SmlI CTYRAG 4 cut(s) 721, 1238, 1376, 1862
SmoI CTYRAG 4 cut(s) 721, 1238, 1376, 1862
Sse9I AATT 6 cut(s) 54, 761, 876, 1253, 1919, 2016
SsiI CCGC 2 cut(s) 274, 328
SspI AATATT 1 cut(s) 1183
SspMI CTAG 4 cut(s) 791, 993, 1475, 1814
StyD4I CCNGG 5 cut(s) 414, 851, 999, 1498, 1725
TaaI ACNGT 8 cut(s) 232, 350, 1135, 1398, 1460, 1516, 1522, 1885
TaiI ACGT 2 cut(s) 519, 1649
TaqI TCGA 4 cut(s) 1227, 1464, 1704, 1995
TasI AATT 6 cut(s) 54, 761, 876, 1253, 1919, 2016
TatI WGTACW 3 cut(s) 751, 858, 1848
TfiI GAWTC 5 cut(s) 73, 634, 644, 1941, 2188
Tru1I TTAA 8 cut(s) 222, 285, 912, 1709, 1749, 1766, 1863, 2153
Tru9I TTAA 8 cut(s) 222, 285, 912, 1709, 1749, 1766, 1863, 2153
TscAI CASTG 9 cut(s) 387, 733, 1342, 1443, 1525, 1537, 1582, 1665, 1780
TseFI GTSAC 7 cut(s) 410, 922, 1057, 1271, 1423, 1516, 1670
TseI GCWGC 6 cut(s) 181, 271, 916, 1490, 1730, 2024
Tsp45I GTSAC 7 cut(s) 410, 922, 1057, 1271, 1423, 1516, 1670
TspGWI ACGGA 3 cut(s) 85, 398, 1442
TspRI CASTG 9 cut(s) 387, 733, 1342, 1443, 1525, 1537, 1582, 1665, 1780
Vha464I CTTAAG 1 cut(s) 1862
VneI GTGCAC 1 cut(s) 148
XapI RAATTY 2 cut(s) 876, 1919
XbaI TCTAGA 1 cut(s) 790
XceI RCATGY 5 cut(s) 148, 405, 664, 923, 2200
XcmI CCANNNNNNNNNTGG 1 cut(s) 2167
XmiI GTMKAC 1 cut(s) 1718
XmnI GAANNNNTTC 1 cut(s) 1008
XspI CTAG 4 cut(s) 791, 993, 1475, 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.