Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 432 |
| Acc65I | GGTACC | 1 cut(s) | 267 |
| AccB1I | GGYRCC | 3 cut(s) | 49, 173, 267 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 716 |
| AciI | CCGC | 1 cut(s) | 252 |
| AclWI | GGATC | 1 cut(s) | 412 |
| AcsI | RAATTY | 3 cut(s) | 300, 736, 848 |
| AfaI | GTAC | 4 cut(s) | 269, 484, 512, 648 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 716 |
| AgsI | TTSAA | 2 cut(s) | 443, 934 |
| AhlI | ACTAGT | 1 cut(s) | 764 |
| AjnI | CCWGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| AluBI | AGCT | 7 cut(s) | 31, 107, 119, 151, 545, 783, 928 |
| AluI | AGCT | 7 cut(s) | 31, 107, 119, 151, 545, 783, 928 |
| Alw21I | GWGCWC | 1 cut(s) | 121 |
| Alw26I | GTCTC | 3 cut(s) | 150, 508, 567 |
| AlwI | GGATC | 1 cut(s) | 412 |
| AoxI | GGCC | 2 cut(s) | 109, 702 |
| ApeKI | GCWGC | 2 cut(s) | 28, 68 |
| ApoI | RAATTY | 3 cut(s) | 300, 736, 848 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 848 |
| Asp718I | GGTACC | 1 cut(s) | 267 |
| AspLEI | GCGC | 1 cut(s) | 41 |
| AspS9I | GGNCC | 1 cut(s) | 110 |
| AsuHPI | GGTGA | 3 cut(s) | 120, 377, 488 |
| AsuII | TTCGAA | 1 cut(s) | 852 |
| BaeGI | GKGCMC | 1 cut(s) | 178 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 251, 284 |
| BanI | GGYRCC | 3 cut(s) | 49, 173, 267 |
| BanII | GRGCYC | 1 cut(s) | 121 |
| Bbv12I | GWGCWC | 1 cut(s) | 121 |
| BbvI | GCAGC | 2 cut(s) | 40, 80 |
| BccI | CCATC | 3 cut(s) | 473, 527, 721 |
| BciT130I | CCWGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| BcoDI | GTCTC | 3 cut(s) | 150, 508, 567 |
| BcuI | ACTAGT | 1 cut(s) | 764 |
| BfaI | CTAG | 4 cut(s) | 32, 344, 357, 765 |
| BisI | GCNGC | 2 cut(s) | 29, 69 |
| BlsI | GCNGC | 2 cut(s) | 30, 70 |
| Bme1390I | CCNGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| BmgT120I | GGNCC | 1 cut(s) | 110 |
| BmiI | GGNNCC | 3 cut(s) | 51, 175, 269 |
| BmrFI | CCNGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| BmsI | GCATC | 3 cut(s) | 50, 343, 616 |
| BpmI | CTGGAG | 2 cut(s) | 135, 443 |
| Bpu14I | TTCGAA | 1 cut(s) | 852 |
| BsaBI | GATNNNNATC | 1 cut(s) | 195 |
| BsaJI | CCNNGG | 4 cut(s) | 112, 169, 263, 715 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 716 |
| Bse1I | ACTGG | 1 cut(s) | 51 |
| Bse3DI | GCAATG | 1 cut(s) | 670 |
| Bse8I | GATNNNNATC | 1 cut(s) | 195 |
| BseBI | CCWGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| BseDI | CCNNGG | 4 cut(s) | 112, 169, 263, 715 |
| BseGI | GGATG | 1 cut(s) | 334 |
| BseJI | GATNNNNATC | 1 cut(s) | 195 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 716 |
| BseMI | GCAATG | 1 cut(s) | 670 |
| BseMII | CTCAG | 3 cut(s) | 161, 173, 243 |
| BseNI | ACTGG | 1 cut(s) | 51 |
| BseSI | GKGCMC | 1 cut(s) | 178 |
| BseXI | GCAGC | 2 cut(s) | 40, 80 |
| BshFI | GGCC | 2 cut(s) | 111, 704 |
| BshNI | GGYRCC | 3 cut(s) | 49, 173, 267 |
| BsiHKAI | GWGCWC | 1 cut(s) | 121 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 716 |
| BsmAI | GTCTC | 3 cut(s) | 150, 508, 567 |
| BsmBI | CGTCTC | 1 cut(s) | 150 |
| BsnI | GGCC | 2 cut(s) | 111, 704 |
| Bsp119I | TTCGAA | 1 cut(s) | 852 |
| Bsp1286I | GDGCHC | 2 cut(s) | 121, 178 |
| Bsp143I | GATC | 2 cut(s) | 190, 417 |
| Bsp19I | CCATGG | 1 cut(s) | 715 |
| BspACI | CCGC | 1 cut(s) | 252 |
| BspANI | GGCC | 2 cut(s) | 111, 704 |
| BspCNI | CTCAG | 3 cut(s) | 160, 172, 242 |
| BspHI | TCATGA | 1 cut(s) | 636 |
| BspLI | GGNNCC | 3 cut(s) | 51, 175, 269 |
| BspPI | GGATC | 1 cut(s) | 412 |
| BspT104I | TTCGAA | 1 cut(s) | 852 |
| BspT107I | GGYRCC | 3 cut(s) | 49, 173, 267 |
| BsrDI | GCAATG | 1 cut(s) | 670 |
| BsrI | ACTGG | 1 cut(s) | 51 |
| BssECI | CCNNGG | 4 cut(s) | 112, 169, 263, 715 |
| BssMI | GATC | 2 cut(s) | 190, 417 |
| BssT1I | CCWWGG | 1 cut(s) | 715 |
| Bst2UI | CCWGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| Bst6I | CTCTTC | 1 cut(s) | 160 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 902 |
| BstBI | TTCGAA | 1 cut(s) | 852 |
| BstC8I | GCNNGC | 1 cut(s) | 109 |
| BstDEI | CTNAG | 3 cut(s) | 147, 159, 229 |
| BstDSI | CCRYGG | 1 cut(s) | 715 |
| BstF5I | GGATG | 1 cut(s) | 334 |
| BstHHI | GCGC | 1 cut(s) | 41 |
| BstKTI | GATC | 2 cut(s) | 193, 420 |
| BstMAI | GTCTC | 3 cut(s) | 150, 508, 567 |
| BstMBI | GATC | 2 cut(s) | 190, 417 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 58, 869, 902 |
| BstNI | CCWGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| BstNSI | RCATGY | 3 cut(s) | 23, 900, 943 |
| BstSCI | CCNGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| BstSLI | GKGCMC | 1 cut(s) | 178 |
| BstV1I | GCAGC | 2 cut(s) | 40, 80 |
| BstX2I | RGATCY | 1 cut(s) | 417 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 892 |
| BstYI | RGATCY | 1 cut(s) | 417 |
| BsuRI | GGCC | 2 cut(s) | 111, 704 |
| BtgI | CCRYGG | 1 cut(s) | 715 |
| BtsCI | GGATG | 1 cut(s) | 334 |
| BtsIMutI | CAGTG | 1 cut(s) | 44 |
| Cac8I | GCNNGC | 1 cut(s) | 109 |
| CciI | TCATGA | 1 cut(s) | 636 |
| CfoI | GCGC | 1 cut(s) | 41 |
| Cfr13I | GGNCC | 1 cut(s) | 110 |
| CseI | GACGC | 1 cut(s) | 132 |
| Csp6I | GTAC | 4 cut(s) | 268, 483, 511, 647 |
| CviAII | CATG | 7 cut(s) | 20, 25, 496, 637, 716, 897, 940 |
| CviQI | GTAC | 4 cut(s) | 268, 483, 511, 647 |
| DdeI | CTNAG | 3 cut(s) | 147, 159, 229 |
| DpnI | GATC | 2 cut(s) | 192, 419 |
| DpnII | GATC | 2 cut(s) | 190, 417 |
| DraI | TTTAAA | 1 cut(s) | 376 |
| Eam1104I | CTCTTC | 1 cut(s) | 160 |
| EarI | CTCTTC | 1 cut(s) | 160 |
| EciI | GGCGGA | 1 cut(s) | 241 |
| Ecl136II | GAGCTC | 1 cut(s) | 119 |
| Eco130I | CCWWGG | 1 cut(s) | 715 |
| Eco147I | AGGCCT | 1 cut(s) | 704 |
| Eco24I | GRGCYC | 1 cut(s) | 121 |
| Eco53kI | GAGCTC | 1 cut(s) | 119 |
| EcoICRI | GAGCTC | 1 cut(s) | 119 |
| EcoRI | GAATTC | 2 cut(s) | 300, 848 |
| EcoRII | CCWGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| EcoT14I | CCWWGG | 1 cut(s) | 715 |
| EcoT38I | GRGCYC | 1 cut(s) | 121 |
| ErhI | CCWWGG | 1 cut(s) | 715 |
| Esp3I | CGTCTC | 1 cut(s) | 150 |
| FaeI | CATG | 7 cut(s) | 23, 28, 499, 640, 719, 900, 943 |
| FatI | CATG | 7 cut(s) | 19, 24, 495, 636, 715, 896, 939 |
| Fnu4HI | GCNGC | 2 cut(s) | 29, 69 |
| FokI | GGATG | 1 cut(s) | 341 |
| FriOI | GRGCYC | 1 cut(s) | 121 |
| Fsp4HI | GCNGC | 2 cut(s) | 29, 69 |
| FspBI | CTAG | 4 cut(s) | 32, 344, 357, 765 |
| GlaI | GCGC | 1 cut(s) | 40 |
| GluI | GCNGC | 2 cut(s) | 29, 69 |
| GsuI | CTGGAG | 2 cut(s) | 135, 443 |
| HaeIII | GGCC | 2 cut(s) | 111, 704 |
| HgaI | GACGC | 1 cut(s) | 132 |
| HhaI | GCGC | 1 cut(s) | 41 |
| Hin1II | CATG | 7 cut(s) | 23, 28, 499, 640, 719, 900, 943 |
| Hin6I | GCGC | 1 cut(s) | 39 |
| HinP1I | GCGC | 1 cut(s) | 39 |
| HindIII | AAGCTT | 1 cut(s) | 781 |
| HphI | GGTGA | 3 cut(s) | 120, 377, 488 |
| Hpy188I | TCNGA | 2 cut(s) | 162, 618 |
| Hpy188III | TCNNGA | 3 cut(s) | 637, 805, 934 |
| HpyAV | CCTTC | 5 cut(s) | 141, 224, 287, 406, 437 |
| HpyCH4V | TGCA | 3 cut(s) | 28, 593, 905 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 58, 869, 902 |
| HpyF3I | CTNAG | 3 cut(s) | 147, 159, 229 |
| Hsp92II | CATG | 7 cut(s) | 23, 28, 499, 640, 719, 900, 943 |
| HspAI | GCGC | 1 cut(s) | 39 |
| KpnI | GGTACC | 1 cut(s) | 271 |
| Kzo9I | GATC | 2 cut(s) | 190, 417 |
| LmnI | GCTCC | 2 cut(s) | 116, 925 |
| Lsp1109I | GCAGC | 2 cut(s) | 40, 80 |
| LweI | GCATC | 3 cut(s) | 50, 343, 616 |
| MaeI | CTAG | 4 cut(s) | 32, 344, 357, 765 |
| MaeIII | GTNAC | 1 cut(s) | 8 |
| MalI | GATC | 2 cut(s) | 192, 419 |
| MboI | GATC | 2 cut(s) | 190, 417 |
| MboII | GAAGA | 1 cut(s) | 147 |
| MflI | RGATCY | 1 cut(s) | 417 |
| MhlI | GDGCHC | 2 cut(s) | 121, 178 |
| MluCI | AATT | 7 cut(s) | 138, 300, 402, 427, 736, 848, 910 |
| MnlI | CCTC | 4 cut(s) | 290, 577, 694, 828 |
| MroXI | GAANNNNTTC | 1 cut(s) | 848 |
| MseI | TTAA | 6 cut(s) | 63, 375, 396, 407, 492, 909 |
| MslI | CAYNNNNRTG | 2 cut(s) | 635, 890 |
| MspR9I | CCNGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| MvaI | CCWGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 58, 869, 902 |
| NcoI | CCATGG | 1 cut(s) | 715 |
| NdeII | GATC | 2 cut(s) | 190, 417 |
| NlaIII | CATG | 7 cut(s) | 23, 28, 499, 640, 719, 900, 943 |
| NlaIV | GGNNCC | 3 cut(s) | 51, 175, 269 |
| NmuCI | GTSAC | 1 cut(s) | 8 |
| NspI | RCATGY | 3 cut(s) | 23, 900, 943 |
| NspV | TTCGAA | 1 cut(s) | 852 |
| PagI | TCATGA | 1 cut(s) | 636 |
| PceI | AGGCCT | 1 cut(s) | 704 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 199 |
| PdmI | GAANNNNTTC | 1 cut(s) | 848 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 716 |
| PfoI | TCCNGGA | 1 cut(s) | 420 |
| PkrI | GCNGC | 2 cut(s) | 30, 70 |
| PsiI | TTATAA | 1 cut(s) | 432 |
| Psp124BI | GAGCTC | 1 cut(s) | 121 |
| Psp6I | CCWGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| PspGI | CCWGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| PspN4I | GGNNCC | 3 cut(s) | 51, 175, 269 |
| PspPI | GGNCC | 1 cut(s) | 110 |
| PsuI | RGATCY | 1 cut(s) | 417 |
| RsaI | GTAC | 4 cut(s) | 269, 484, 512, 648 |
| RsaNI | GTAC | 4 cut(s) | 268, 483, 511, 647 |
| RseI | CAYNNNNRTG | 2 cut(s) | 635, 890 |
| SacI | GAGCTC | 1 cut(s) | 121 |
| SaqAI | TTAA | 6 cut(s) | 63, 375, 396, 407, 492, 909 |
| SatI | GCNGC | 2 cut(s) | 29, 69 |
| Sau3AI | GATC | 2 cut(s) | 190, 417 |
| Sau96I | GGNCC | 1 cut(s) | 110 |
| ScrFI | CCNGG | 5 cut(s) | 114, 171, 265, 422, 576 |
| SduI | GDGCHC | 2 cut(s) | 121, 178 |
| SfaNI | GCATC | 3 cut(s) | 50, 343, 616 |
| SfuI | TTCGAA | 1 cut(s) | 852 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 635, 890 |
| SpeI | ACTAGT | 1 cut(s) | 764 |
| Sse9I | AATT | 7 cut(s) | 138, 300, 402, 427, 736, 848, 910 |
| SseBI | AGGCCT | 1 cut(s) | 704 |
| SsiI | CCGC | 1 cut(s) | 252 |
| SspI | AATATT | 1 cut(s) | 372 |
| SspMI | CTAG | 4 cut(s) | 32, 344, 357, 765 |
| SstI | GAGCTC | 1 cut(s) | 121 |
| StuI | AGGCCT | 1 cut(s) | 704 |
| StyD4I | CCNGG | 5 cut(s) | 112, 169, 263, 420, 574 |
| StyI | CCWWGG | 1 cut(s) | 715 |
| TaqI | TCGA | 4 cut(s) | 383, 660, 852, 889 |
| TasI | AATT | 7 cut(s) | 138, 300, 402, 427, 736, 848, 910 |
| Tru1I | TTAA | 6 cut(s) | 63, 375, 396, 407, 492, 909 |
| Tru9I | TTAA | 6 cut(s) | 63, 375, 396, 407, 492, 909 |
| TscAI | CASTG | 1 cut(s) | 51 |
| TseFI | GTSAC | 1 cut(s) | 8 |
| TseI | GCWGC | 2 cut(s) | 28, 68 |
| Tsp45I | GTSAC | 1 cut(s) | 8 |
| TspDTI | ATGAA | 4 cut(s) | 425, 625, 653, 824 |
| TspGWI | ACGGA | 1 cut(s) | 341 |
| TspRI | CASTG | 1 cut(s) | 51 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 716 |
| XapI | RAATTY | 3 cut(s) | 300, 736, 848 |
| XceI | RCATGY | 3 cut(s) | 23, 900, 943 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 271 |
| XmnI | GAANNNNTTC | 1 cut(s) | 848 |
| XspI | CTAG | 4 cut(s) | 32, 344, 357, 765 |