RLG00000033929

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000162
Physical Location & Seq
Forward (+)
34309695 .. 34315532
5838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033929

Sequence Viewer

Length: 2265 bp
ATGGGACATGATACTTGTAGAAGCATACATGAGGCTGTATACTTCGTGGATCAGTTGCATCGGAACATAGAGAATTTGGATTTCAATTCAATCTCCGATGACCAATTTCCCAATTGGTGCAACTCGTTGCCGCCAGTGGTGTCCTCTCCACCTTCAGGCAGCGGTGGTCCTCTCAATACCAGCTACCGTAATAATGTTGCAACCAACACTGTGACAGCTCTATCGGAGGACAACGAGAGGCTGAGGCGGAGCAACAACATACTTATGTCCGAGTTCAACCTTATGAGAAAGCTCTACAACAATATCATCTACTTCGTTCAGAACCATGTCAAGCCTGTCGCTCCAAGCAATTGTTACCCAGGTTCTCTACTTCTTTGCAACCCATCACCAAACTCACACAATCCCAACTCTTCTGCTGCCATAAACAAGTTTTTGAACCACGTTCTTGGGTACTACCCTGAGTGGGCTATCGTCAAGCTAACCCGAATCCTCGAGGGGTCGCCGGAGACGCCGTTCGACGCCGAATACCACATCTATGTCTACTCCACCGTCTACAACATGTGCGACGATCACTCCCAGCAGGTCTACGAGGCTTGTCGCGAGACCATCGAGGCCTACAACACTGAGATTGTGTTGCCTGGTTTAGCAGACAAGCACGGCGCGCTTTTGCTCCTGGAGTTCGTCAGAAGGTGGAGGAATCACAAGGCTCTGATGCGGTGGCTATGGCGTTTCTTTATTGTTCTCGATCAGTATTATGTTGAGAAAGCAAAGGTTCCTGGGATTAAGCAGGCTGGGATTATCGGGTTTCGCTATGAGGAATGCTTGAGGAGGGAGAAGGAGAGAGTATTGCATTACCTGCATTCGAGCAGTGAGGAGAAGTTGTTGAAGAAAGTGCACTATGAGCTGGTGGTGGTTTATGCACACCAACTGCTCGATGAAAGGGATTCTGGGTGTAGTGCTTTGCTTCGAGATAATAAGGTGGAGGATCTGGCTAGGATGTATAGGCTTTACAGTAGAACACCTAAAGAGTTGGAACTTGTTGTTAATGTGTTCAGACAATATGTTACTGATGAAGGTAAAGCGTTTGTGCAACAAGCTGAAGAAGCTGCAAGTAACCAGCAGGCTTCAAATGCAGAGCATGGGGTGGTCCTTATCAGAAAAATAATTGAAATATATGACAAGTATGTGGGATATAGAGGCGTTTGTGGGAGTCCGAGTGCTGAATTGCTTGCTGGATTCTGTGATAGTATTCTTAAGAAGGGTGGCTGTCAGCTTAGTAATGAGGCCATAGATGAAAGTTTTGAGAAGGTTGTTAAGGTGCTTGCTTATTTTAATGAGAAAGATGTTCTTGCAGAGTTCTGCAGGAAAAAACCGGCTTGCCGGTTGCTCTTTGATCGGAGTGCCAATGATGACCGTGAGAATAGTTTTCTAACAAAGCTGAAGCAGATGCAAAGATGGAAGGAATGGTGTGTCACAGATTTAACATTGGCTAAGGACACTCAGACCAACTTCAAAAATTTTCTTGACAGCAATCCAAATTTACAACCAGGGATTGATTTGACGGTCACTATTCTGACAACTGGTTACTGGCCAAGTTACAAATCAACTGATCTTAACCTTCCTGAAGAGATGGTCAAGTGTGTTGAAGTTTTCTATGAATTCTTTGACACACAAAAGAAATTGAGAAAGCTTTCATGGATTTACTCCTTGGGTACTTGCATTGTCATTGCCAAGTTTGAACCAAAAACCATGGAATTGGTTGCGTCGACATATCAGGCTGCTCTCCTTCTGCTCTTCAATTATGCTGATAGATTGAGCTATTCAGAAATATTGAATCAGTTGAACCTCACCAATGAGGACTTGGTTAGATTGCTTCATTCGTTGTCATGTGCCAAGTACAAGATCCTCATTAAGGAACCAAATACCAAGGCTATCTCACCAAATGACGACTTTATGTTCAACTATAAGTTCACTGATAAGATGAAAACAATTGAGATTCCTCTCCCACCAGTTGATGAAAGGACGAAGGGGATTGAGGAAGTTGACAAAGACAGGCGATATGCCATTGATGCTGCAATTGTAAGGATTATGAAGAGTAGGAAAATTTTGGACCATCAAGAATTAGTCATGTGGTCTGTTGAGCAGTTGCAGCGCATGTTCAAGCCAGACATTACAGCGATTAAGAAGCGTATTGAAGATCTTATCACACGTGAATACCTGGAGAGGGACGAGGAAAACCCTACCATCTTCAAGTATCTTGCATGA

Protein Analysis

755

Amino Acids

87.4

Weight (kDa)

6.33

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 179 - 270 9.5e-12 Cullin alpha solenoid domain
Cullin PF00888 271 - 395 6.9e-21 Cullin alpha solenoid domain
Cullin PF00888 405 - 482 7.7e-18 Cullin alpha solenoid domain
Cullin_AB PF26557 524 - 657 6.4e-40 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 684 - 746 3.8e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 491
Acc36I ACCTGC 2 cut(s) 571, 864
AccI GTMKAC 5 cut(s) 39, 540, 552, 585, 1766
AccII CGCG 2 cut(s) 600, 662
AciI CCGC 4 cut(s) 131, 162, 247, 715
AclWI GGATC 3 cut(s) 57, 993, 1897
AcoI YGGCCR 1 cut(s) 1589
AcsI RAATTY 5 cut(s) 73, 1516, 1537, 1658, 2103
AcuI CTGAAG 4 cut(s) 138, 1119, 1460, 1644
AcvI CACGTG 1 cut(s) 2210
AcyI GRCGYC 2 cut(s) 509, 519
AfaI GTAC 3 cut(s) 452, 1714, 1898
AfiI CCNNNNNNNGG 4 cut(s) 155, 463, 1912, 2224
AflII CTTAAG 1 cut(s) 1253
AflIII ACRYGT 2 cut(s) 558, 2207
AjnI CCWGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 2 cut(s) 500, 596
Alw44I GTGCAC 1 cut(s) 893
AlwI GGATC 3 cut(s) 57, 993, 1897
Ama87I CYCGRG 1 cut(s) 491
AoxI GGCC 3 cut(s) 612, 1284, 1589
ApaLI GTGCAC 1 cut(s) 893
ApeKI GCWGC 6 cut(s) 159, 416, 1106, 1778, 2072, 2149
ApoI RAATTY 5 cut(s) 73, 1516, 1537, 1658, 2103
Asp700I GAANNNNTTC 1 cut(s) 1690
AspLEI GCGC 3 cut(s) 662, 664, 2154
AspS9I GGNCC 3 cut(s) 167, 1147, 2110
AsuHPI GGTGA 3 cut(s) 378, 1840, 1929
AvaI CYCGRG 1 cut(s) 491
AvaII GGWCC 3 cut(s) 167, 1147, 2110
BaeGI GKGCMC 1 cut(s) 897
BalI TGGCCA 1 cut(s) 1591
BbrPI CACGTG 1 cut(s) 2210
Bbv12I GWGCWC 1 cut(s) 897
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 6 cut(s) 171, 403, 1093, 1765, 2059, 2161
BccI CCATC 6 cut(s) 391, 614, 1449, 1624, 2121, 2252
BceAI ACGGC 2 cut(s) 496, 673
BciT130I CCWGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
BcoDI GTCTC 2 cut(s) 500, 596
BfaI CTAG 1 cut(s) 993
BfmI CTRYAG 1 cut(s) 1360
BfrI CTTAAG 1 cut(s) 1253
BfuAI ACCTGC 2 cut(s) 571, 864
BglII AGATCT 1 cut(s) 2197
BisI GCNGC 7 cut(s) 131, 160, 417, 1107, 1779, 2073, 2150
BlsI GCNGC 7 cut(s) 132, 161, 418, 1108, 1780, 2074, 2151
Bme1390I CCNGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
Bme18I GGWCC 3 cut(s) 167, 1147, 2110
BmeT110I CYCGRG 1 cut(s) 491
BmgT120I GGNCC 3 cut(s) 167, 1147, 2110
BmiI GGNNCC 2 cut(s) 774, 1917
BmrFI CCNGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
BmsI GCATC 4 cut(s) 67, 702, 1437, 2059
BpmI CTGGAG 2 cut(s) 695, 2240
Bpu10I CCTNAGC 2 cut(s) 242, 1491
BpuEI CTTGAG 1 cut(s) 844
BsaAI YACGTR 1 cut(s) 2210
BsaHI GRCGYC 2 cut(s) 509, 519
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 6 cut(s) 358, 776, 1547, 1707, 1749, 1926
BsaXI ACNNNNNCTCC 8 cut(s) 497, 527, 557, 587, 866, 896, 1201, 1231
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 463, 1912, 2224
Bse118I RCCGGY 2 cut(s) 1372, 1380
Bse1I ACTGG 4 cut(s) 134, 1585, 1592, 2009
Bse3DI GCAATG 1 cut(s) 1725
BseBI CCWGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
BseDI CCNNGG 6 cut(s) 358, 776, 1547, 1707, 1749, 1926
BseGI GGATG 1 cut(s) 1002
BseLI CCNNNNNNNGG 4 cut(s) 155, 463, 1912, 2224
BseMI GCAATG 1 cut(s) 1725
BseMII CTCAG 4 cut(s) 233, 450, 615, 1514
BseNI ACTGG 4 cut(s) 134, 1585, 1592, 2009
BsePI GCGCGC 1 cut(s) 660
BseRI GAGGAG 2 cut(s) 841, 887
BseSI GKGCMC 1 cut(s) 897
BseXI GCAGC 6 cut(s) 171, 403, 1093, 1765, 2059, 2161
BseYI CCCAGC 2 cut(s) 576, 791
Bsh1236I CGCG 2 cut(s) 600, 662
BshFI GGCC 3 cut(s) 614, 1286, 1591
BsiHKAI GWGCWC 1 cut(s) 897
BsiHKCI CYCGRG 1 cut(s) 491
BsiSI CCGG 3 cut(s) 503, 1373, 1381
BslFI GGGAC 2 cut(s) 18, 2240
BslI CCNNNNNNNGG 4 cut(s) 155, 463, 1912, 2224
BsmAI GTCTC 2 cut(s) 500, 596
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 18, 2240
BsmI GAATGC 2 cut(s) 824, 859
BsnI GGCC 3 cut(s) 614, 1286, 1591
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
Bsp19I CCATGG 1 cut(s) 1749
Bsp68I TCGCGA 1 cut(s) 600
BspACI CCGC 4 cut(s) 131, 162, 247, 715
BspANI GGCC 3 cut(s) 614, 1286, 1591
BspCNI CTCAG 4 cut(s) 234, 451, 616, 1513
BspFNI CGCG 2 cut(s) 600, 662
BspLI GGNNCC 2 cut(s) 774, 1917
BspMAI CTGCAG 1 cut(s) 1364
BspMI ACCTGC 2 cut(s) 571, 864
BspPI GGATC 3 cut(s) 57, 993, 1897
BspQI GCTCTTC 1 cut(s) 1799
BspTI CTTAAG 1 cut(s) 1253
BspTNI GGTCTC 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 1725
BsrFI RCCGGY 2 cut(s) 1372, 1380
BsrI ACTGG 4 cut(s) 134, 1585, 1592, 2009
BssAI RCCGGY 2 cut(s) 1372, 1380
BssECI CCNNGG 6 cut(s) 358, 776, 1547, 1707, 1749, 1926
BssHII GCGCGC 1 cut(s) 660
BssMI GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
BssNAI GTATAC 1 cut(s) 40
BssNI GRCGYC 2 cut(s) 509, 519
BssT1I CCWWGG 3 cut(s) 1707, 1749, 1926
Bst1107I GTATAC 1 cut(s) 40
Bst2UI CCWGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
Bst4CI ACNGT 6 cut(s) 188, 211, 550, 1013, 1415, 1564
Bst6I CTCTTC 4 cut(s) 415, 1620, 1799, 2087
BstACI GRCGYC 2 cut(s) 509, 519
BstAFI CTTAAG 1 cut(s) 1253
BstAPI GCANNNNNTGC 1 cut(s) 856
BstBAI YACGTR 1 cut(s) 2210
BstC8I GCNNGC 6 cut(s) 662, 789, 1122, 1230, 1323, 1378
BstDEI CTNAG 6 cut(s) 242, 459, 624, 1274, 1491, 1500
BstDSI CCRYGG 1 cut(s) 1749
BstENI CCTNNNNNAGG 1 cut(s) 1910
BstF5I GGATG 1 cut(s) 1002
BstFNI CGCG 2 cut(s) 600, 662
BstHHI GCGC 3 cut(s) 662, 664, 2154
BstKTI GATC 8 cut(s) 52, 571, 748, 988, 1396, 1612, 1905, 2200
BstMAI GTCTC 2 cut(s) 500, 596
BstMBI GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
BstMWI GCNNNNNNNGC 8 cut(s) 508, 661, 856, 901, 1103, 1130, 2069, 2149
BstNI CCWGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
BstNSI RCATGY 2 cut(s) 562, 2158
BstSCI CCNGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
BstSFI CTRYAG 1 cut(s) 1360
BstSLI GKGCMC 1 cut(s) 897
BstUI CGCG 2 cut(s) 600, 662
BstV1I GCAGC 6 cut(s) 171, 403, 1093, 1765, 2059, 2161
BstX2I RGATCY 3 cut(s) 985, 1902, 2197
BstXI CCANNNNNNTGG 2 cut(s) 446, 1756
BstYI RGATCY 3 cut(s) 985, 1902, 2197
BstZ17I GTATAC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 614, 1286, 1591
BtgI CCRYGG 1 cut(s) 1749
BtsCI GGATG 1 cut(s) 1002
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 5 cut(s) 141, 207, 621, 874, 1971
BtuMI TCGCGA 1 cut(s) 600
BveI ACCTGC 2 cut(s) 571, 864
Cac8I GCNNGC 6 cut(s) 662, 789, 1122, 1230, 1323, 1378
CfoI GCGC 3 cut(s) 662, 664, 2154
Cfr10I RCCGGY 2 cut(s) 1372, 1380
Cfr13I GGNCC 3 cut(s) 167, 1147, 2110
CseI GACGC 3 cut(s) 517, 527, 1752
Csp6I GTAC 3 cut(s) 451, 1713, 1897
CviQI GTAC 3 cut(s) 451, 1713, 1897
DdeI CTNAG 6 cut(s) 242, 459, 624, 1274, 1491, 1500
DpnI GATC 8 cut(s) 51, 570, 747, 987, 1395, 1611, 1904, 2199
DpnII GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
EaeI YGGCCR 1 cut(s) 1589
Eam1104I CTCTTC 4 cut(s) 415, 1620, 1799, 2087
EarI CTCTTC 4 cut(s) 415, 1620, 1799, 2087
EciI GGCGGA 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 1707, 1749, 1926
Eco147I AGGCCT 1 cut(s) 614
Eco31I GGTCTC 1 cut(s) 596
Eco47I GGWCC 3 cut(s) 167, 1147, 2110
Eco57I CTGAAG 4 cut(s) 138, 1119, 1460, 1644
Eco72I CACGTG 1 cut(s) 2210
Eco88I CYCGRG 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 1910
EcoRI GAATTC 1 cut(s) 1658
EcoRII CCWGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
EcoT14I CCWWGG 3 cut(s) 1707, 1749, 1926
ErhI CCWWGG 3 cut(s) 1707, 1749, 1926
Esp3I CGTCTC 1 cut(s) 500
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FaqI GGGAC 2 cut(s) 18, 2240
FblI GTMKAC 5 cut(s) 39, 540, 552, 585, 1766
Fnu4HI GCNGC 7 cut(s) 131, 160, 417, 1107, 1779, 2073, 2150
FokI GGATG 1 cut(s) 1009
Fsp4HI GCNGC 7 cut(s) 131, 160, 417, 1107, 1779, 2073, 2150
FspBI CTAG 1 cut(s) 993
GlaI GCGC 3 cut(s) 661, 663, 2153
GluI GCNGC 7 cut(s) 131, 160, 417, 1107, 1779, 2073, 2150
GsaI CCCAGC 2 cut(s) 580, 795
GsuI CTGGAG 2 cut(s) 695, 2240
HaeIII GGCC 3 cut(s) 614, 1286, 1591
HapII CCGG 3 cut(s) 503, 1373, 1381
HgaI GACGC 3 cut(s) 517, 527, 1752
HhaI GCGC 3 cut(s) 662, 664, 2154
Hin1I GRCGYC 2 cut(s) 509, 519
Hin6I GCGC 3 cut(s) 660, 662, 2152
HinP1I GCGC 3 cut(s) 660, 662, 2152
HincII GTYRAC 2 cut(s) 1767, 2044
HindII GTYRAC 2 cut(s) 1767, 2044
HindIII AAGCTT 1 cut(s) 1688
HinfI GANTC 7 cut(s) 486, 697, 944, 1210, 1236, 1834, 1996
HpaII CCGG 3 cut(s) 503, 1373, 1381
HphI GGTGA 3 cut(s) 378, 1840, 1929
Hpy166II GTNNAC 8 cut(s) 40, 541, 553, 586, 895, 1767, 1971, 2044
Hpy188III TCNNGA 6 cut(s) 599, 743, 968, 1523, 1622, 2117
Hpy8I GTNNAC 8 cut(s) 40, 541, 553, 586, 895, 1767, 1971, 2044
Hpy99I CGWCG 3 cut(s) 521, 569, 1768
HpyCH4III ACNGT 6 cut(s) 188, 211, 550, 1013, 1415, 1564
HpyCH4IV ACGT 2 cut(s) 441, 2209
HpyF10VI GCNNNNNNNGC 8 cut(s) 508, 661, 856, 901, 1103, 1130, 2069, 2149
HpyF3I CTNAG 6 cut(s) 242, 459, 624, 1274, 1491, 1500
HpySE526I ACGT 2 cut(s) 441, 2209
Hsp92I GRCGYC 2 cut(s) 509, 519
HspAI GCGC 3 cut(s) 660, 662, 2152
Kzo9I GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
LguI GCTCTTC 1 cut(s) 1799
LmnI GCTCC 3 cut(s) 249, 346, 675
Lsp1109I GCAGC 6 cut(s) 171, 403, 1093, 1765, 2059, 2161
LweI GCATC 4 cut(s) 67, 702, 1437, 2059
MaeI CTAG 1 cut(s) 993
MaeII ACGT 2 cut(s) 441, 2209
MaeIII GTNAC 8 cut(s) 211, 353, 1063, 1112, 1471, 1564, 1583, 1595
MalI GATC 8 cut(s) 51, 570, 747, 987, 1395, 1611, 1904, 2199
MboI GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
MboII GAAGA 8 cut(s) 402, 898, 1112, 1637, 1786, 2104, 2207, 2239
MfeI CAATTG 4 cut(s) 112, 349, 1989, 2076
MflI RGATCY 3 cut(s) 985, 1902, 2197
MhlI GDGCHC 1 cut(s) 897
MlsI TGGCCA 1 cut(s) 1591
MluNI TGGCCA 1 cut(s) 1591
MlyI GAGTC 1 cut(s) 1219
MmeI TCCRAC 1 cut(s) 1011
Mox20I TGGCCA 1 cut(s) 1591
MroXI GAANNNNTTC 1 cut(s) 1690
MscI TGGCCA 1 cut(s) 1591
MseI TTAA 9 cut(s) 783, 1044, 1254, 1314, 1332, 1481, 1614, 1911, 2181
MslI CAYNNNNRTG 2 cut(s) 263, 534
Msp20I TGGCCA 1 cut(s) 1591
MspA1I CMGCKG 1 cut(s) 162
MspCI CTTAAG 1 cut(s) 1253
MspI CCGG 3 cut(s) 503, 1373, 1381
MspR9I CCNGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
MunI CAATTG 4 cut(s) 112, 349, 1989, 2076
Mva1269I GAATGC 2 cut(s) 824, 859
MvaI CCWGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
MvnI CGCG 2 cut(s) 600, 662
MwoI GCNNNNNNNGC 8 cut(s) 508, 661, 856, 901, 1103, 1130, 2069, 2149
NcoI CCATGG 1 cut(s) 1749
NdeII GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
NlaIV GGNNCC 2 cut(s) 774, 1917
NmuCI GTSAC 3 cut(s) 211, 1471, 1564
NruI TCGCGA 1 cut(s) 600
NspI RCATGY 2 cut(s) 562, 2158
PaeR7I CTCGAG 1 cut(s) 491
PauI GCGCGC 1 cut(s) 660
PceI AGGCCT 1 cut(s) 614
PciI ACATGT 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 1799
PctI GAATGC 2 cut(s) 824, 859
PdmI GAANNNNTTC 1 cut(s) 1690
PfeI GAWTC 6 cut(s) 486, 697, 944, 1236, 1834, 1996
PfoI TCCNGGA 1 cut(s) 672
PkrI GCNGC 7 cut(s) 132, 161, 418, 1108, 1780, 2074, 2151
PleI GAGTC 1 cut(s) 1218
PmaCI CACGTG 1 cut(s) 2210
PmlI CACGTG 1 cut(s) 2210
PpsI GAGTC 1 cut(s) 1218
Ppu21I YACGTR 1 cut(s) 2210
PscI ACATGT 1 cut(s) 558
Psp6I CCWGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
PspCI CACGTG 1 cut(s) 2210
PspFI CCCAGC 2 cut(s) 576, 791
PspGI CCWGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
PspN4I GGNNCC 2 cut(s) 774, 1917
PspPI GGNCC 3 cut(s) 167, 1147, 2110
PspXI VCTCGAGB 1 cut(s) 491
PstI CTGCAG 1 cut(s) 1364
PsuI RGATCY 3 cut(s) 985, 1902, 2197
PteI GCGCGC 1 cut(s) 660
RruI TCGCGA 1 cut(s) 600
RsaI GTAC 3 cut(s) 452, 1714, 1898
RsaNI GTAC 3 cut(s) 451, 1713, 1897
RseI CAYNNNNRTG 2 cut(s) 263, 534
SalI GTCGAC 1 cut(s) 1765
SapI GCTCTTC 1 cut(s) 1799
SaqAI TTAA 9 cut(s) 783, 1044, 1254, 1314, 1332, 1481, 1614, 1911, 2181
SatI GCNGC 7 cut(s) 131, 160, 417, 1107, 1779, 2073, 2150
Sau3AI GATC 8 cut(s) 49, 568, 745, 985, 1393, 1609, 1902, 2197
Sau96I GGNCC 3 cut(s) 167, 1147, 2110
SchI GAGTC 1 cut(s) 1219
ScrFI CCNGG 6 cut(s) 360, 639, 674, 777, 1548, 2219
SduI GDGCHC 1 cut(s) 897
SfaNI GCATC 4 cut(s) 67, 702, 1437, 2059
SfcI CTRYAG 1 cut(s) 1360
Sfr274I CTCGAG 1 cut(s) 491
SinI GGWCC 3 cut(s) 167, 1147, 2110
SlaI CTCGAG 1 cut(s) 491
SmiMI CAYNNNNRTG 2 cut(s) 263, 534
SmlI CTYRAG 3 cut(s) 491, 823, 1253
SmoI CTYRAG 3 cut(s) 491, 823, 1253
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 4 cut(s) 131, 162, 247, 715
SspI AATATT 1 cut(s) 1830
SspMI CTAG 1 cut(s) 993
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 6 cut(s) 358, 637, 672, 775, 1546, 2217
StyI CCWWGG 3 cut(s) 1707, 1749, 1926
TaaI ACNGT 6 cut(s) 188, 211, 550, 1013, 1415, 1564
TaiI ACGT 2 cut(s) 444, 2212
TaqI TCGA 8 cut(s) 492, 516, 609, 744, 863, 933, 967, 1766
TatI WGTACW 1 cut(s) 1896
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 6 cut(s) 486, 697, 944, 1236, 1834, 1996
Tru1I TTAA 9 cut(s) 783, 1044, 1254, 1314, 1332, 1481, 1614, 1911, 2181
Tru9I TTAA 9 cut(s) 783, 1044, 1254, 1314, 1332, 1481, 1614, 1911, 2181
TscAI CASTG 5 cut(s) 141, 214, 628, 874, 1978
TseFI GTSAC 3 cut(s) 211, 1471, 1564
TseI GCWGC 6 cut(s) 159, 416, 1106, 1778, 2072, 2149
Tsp45I GTSAC 3 cut(s) 211, 1471, 1564
TspDTI ATGAA 9 cut(s) 951, 1086, 1308, 1671, 1683, 1865, 1997, 2031, 2105
TspRI CASTG 5 cut(s) 141, 214, 628, 874, 1978
Vha464I CTTAAG 1 cut(s) 1253
VneI GTGCAC 1 cut(s) 893
VpaK11BI GGWCC 3 cut(s) 167, 1147, 2110
XagI CCTNNNNNAGG 1 cut(s) 1910
XapI RAATTY 5 cut(s) 73, 1516, 1537, 1658, 2103
XceI RCATGY 2 cut(s) 562, 2158
XcmI CCANNNNNNNNNTGG 1 cut(s) 1858
XhoI CTCGAG 1 cut(s) 491
XmiI GTMKAC 5 cut(s) 39, 540, 552, 585, 1766
XmnI GAANNNNTTC 1 cut(s) 1690
XspI CTAG 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.