RLG00000033923

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000162
Physical Location & Seq
Forward (+)
34215351 .. 34217594
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033923

Sequence Viewer

Length: 2244 bp
ATGGAGAGAAAAATTATCTTGCTTGATGAAGGATGGGGCCATATACAGAAGGGGATCAGCAAGCTGAAGAGAATTCTCGAAGAACTACCGGAGCCTCAGTTCAGCTCACAGGAATACATGGATCTTTACACAGTGATTTACAACATGTGTGTTCAGAAGACCCCTCACGACTACTCTGAACAGCTCTATATTAATTACGGCGACGTCTTCGTCGAGTACATCGAGTCGACGGTGTTGCCTTCCCTGAGAGAGAAGCACGACGAGTTCATGATGCGTGAGCTCGTCCGAAGATGGTCCAATCACAAACTCATGGTCCGGTGGCTCACGCGCTTCTTTGAGTATCTCGACCGCTACTACGTCGGCCGACGCTCCAAGGATCCTCTCAAGGATGCTGGACTTAAGCGATTTAAGGATCACGTTTACAACGAGATGAATGCAAAGGCTAGGGACTCTCTGATAGCTCTTATTTTGAGGGAACGTGAAGGGGAGCAGATTGACAGAGCGCTGATGAAGAACGTGGTTGATATATTCGTGGAGGTTGGAATGGGAGAGATGGTGAATTACGAGGAGGAGTTCGAAAAACACATGGTTGATGATACTGGGGAATACTACTCTCGTAAGGCCTCGATTTGGATTGTGGAGGATTCTTGTCCCGAGTACATGATGAAGGCGGAGGAGGCCTTGAGGAAGGAGAAGGAGAGAGTTTCCCATTACCTACACTCGGGCAGCGAGCAAAAGCTGTTGGAGAGGGTGCAGCAAGAGTTGTTGGTGGTTCAAGGTCCACAGCTGCTTGATAAAGAGGAGTCTGGATGCCGGACCTTGCTTCGAGACGATAGGGTGGATGACTTGTCTAGGATGTTCAGGCTTTACAATAAAATACCCCAAGGGTTGGATCCTGTTGCTGCTATGTTTAAGAAGCATGTTACTAATGAAGGCACAGCCTTGGTCCAACAGGCCGAAGAGGCAACAGCAGCTGCAAGTGGCAACAAGCAAGGTGGTGGTGCTGGTGAGCCGGGACTAGTATTTATCAGCAAAATACTTGAGCTACATGATAAGTACATGGGATATGTCCTTAGCTGCTTCCAAAACCACTCGCTCTTCCACAAGGCCTTGAAAGAGGCTTTCGAGGTGTTTTGTAATAAATCTATTGCTGGGTACTCGAGTGCGGAATTGCTTGCTACATTCTGTGATAGTATCCTCAAGCGGATCGGTGAGAGTGACGAGGCCATAGAAGAAACTCTTGACAAGATTGTTACGGTGCTTGCATATTTCAGTGACAAGGATCTTTTCGCCGAATTCTGCAGGAAGAAACTTGCCCGTAGGCTGCTCATTCATGATGATAAGAAGAAGCAGAACCGTGCTGACCAGCTTGACAATGAGAGAATGCTTCTAACAAAGCTGAAACAGCAATGTGGAGGACAGTTCACCTCTAAGATGGAGGGAATGGTCACAGATATTACTATTTCTCAGGAAAGCGAGAAGAGTTTCGAAGACTATCTTCAAAAAGATGGTACTCCAAAGCTTGCGTTGGATTTCTCCGTCACTGTTCTAACAACTGGTAACTGGCCAAGCTATAAAACATCTGATCTTCACCTTCCTGAAGAGATGGTCAAGTGCATTGAAGTGTATAAAGAATATTATGACAGAGAAAAGAAACACAGAAAGCTTTCATGGATTTATTCACTGGGCAATTGCCTTGTTAGGGGCAACTTTGAGCCAAAACCCATTGAAATGAATATGTCAACCTATCAGGCTGCTCTCCTGCTGCTCTTCAACACCTCTGAGAAATTGAGCTTTTCAGAGATATTGACTCAATTAAATCTTACCAAAGATGATGTCCACAGAACGCTTCATTCATTTTCTTGTGCCAAGTACAAGATCCTTCTCAAAGAGCCCAACACAAAGACAATCTCCCCCGGTGACACCTTTATGTTCAACTCTAAGTTCACCGACAGAATGAGGAAAATCAGGATCCCTCTGCCACCGCTGCAGGATGAAAGGAAGAAGGTCACGGAAGATGTCGACAAAGAGCGCAAGTATACAATTGACGCTGCACTGGTGAGGATTATGAAGAGTCGAAAAATATTGGGTCATCAACAACTGGTCACTGAGTGTCTTGAACAGTTGCAGCGCACGTTCAAGCCGGAGATCAAAACAATCAAGAGGCGCATTGAAGATCTCATCACCCGTGACTACCTTGAGAGGGACCCTGAGAATCCTAACACATTCAAGTATGTCGCATGA

Protein Analysis

748

Amino Acids

87.31

Weight (kDa)

6.47

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 21 - 491 1.4e-119 Cullin alpha solenoid domain
Cullin_AB PF26557 516 - 649 1.4e-42 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 677 - 739 3.8e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 889
AccI GTMKAC 3 cut(s) 227, 2022, 2039
AccII CGCG 1 cut(s) 328
AciI CCGC 5 cut(s) 349, 671, 1166, 1204, 1985
AcoI YGGCCR 2 cut(s) 361, 1565
AcsI RAATTY 2 cut(s) 72, 1295
AcuI CTGAAG 2 cut(s) 86, 1620
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 2112
AfaI GTAC 6 cut(s) 218, 659, 1058, 1157, 1513, 1874
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1702, 2203
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 1018
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 822
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 3 cut(s) 653, 721, 1159
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 8 cut(s) 37, 361, 621, 678, 954, 1107, 1224, 1565
ApoI RAATTY 2 cut(s) 72, 1295
ArsI GACNNNNNNTTYG 4 cut(s) 297, 329, 1821, 1853
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 2 cut(s) 1484, 1666
AspLEI GCGC 5 cut(s) 330, 505, 2034, 2133, 2169
AspS9I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2206
AsuC2I CCSGG 2 cut(s) 1014, 1917
AsuHPI GGTGA 9 cut(s) 568, 1019, 1223, 1417, 1583, 1931, 1939, 2071, 2176
AsuII TTCGAA 2 cut(s) 576, 1488
AvaI CYCGRG 3 cut(s) 653, 721, 1159
AvaII GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2206
BaeI ACNNNNGTAYC 2 cut(s) 1147, 1180
BalI TGGCCA 1 cut(s) 1567
BamHI GGATCC 3 cut(s) 376, 892, 1971
BanII GRGCYC 2 cut(s) 282, 1896
BbsI GAAGAC 3 cut(s) 164, 199, 1497
Bbv12I GWGCWC 1 cut(s) 282
BccI CCATC 6 cut(s) 27, 285, 547, 1429, 1502, 1600
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 4 cut(s) 217, 251, 416, 450
BciVI GTATCC 1 cut(s) 1205
BcnI CCSGG 2 cut(s) 1014, 1917
BcoDI GTCTC 1 cut(s) 822
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 3 cut(s) 444, 852, 1019
BfmI CTRYAG 2 cut(s) 1300, 1988
BfoI RGCGCY 1 cut(s) 506
BfrI CTTAAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 1205
BglI GCCNNNNNGGC 1 cut(s) 962
BglII AGATCT 1 cut(s) 2176
Bme1390I CCNGG 2 cut(s) 1014, 1917
Bme18I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2206
BmeT110I CYCGRG 3 cut(s) 653, 721, 1159
BmgT120I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2206
BmiI GGNNCC 7 cut(s) 38, 93, 378, 894, 1973, 2207, 2208
BmrFI CCNGG 2 cut(s) 1014, 1917
BmrI ACTGGG 2 cut(s) 609, 1694
BmsI GCATC 3 cut(s) 261, 379, 800
BmuI ACTGGG 2 cut(s) 609, 1694
BpiI GAAGAC 3 cut(s) 164, 199, 1497
Bpu10I CCTNAGC 1 cut(s) 1073
Bpu14I TTCGAA 2 cut(s) 576, 1488
BpuEI CTTGAG 5 cut(s) 368, 703, 1061, 1184, 2219
BpuMI CCSGG 2 cut(s) 1014, 1917
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 372, 883, 942, 1915
BsaWI WCCGGW 2 cut(s) 88, 315
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 540, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1702, 2203
Bse1I ACTGG 6 cut(s) 604, 1561, 1568, 1689, 2061, 2106
Bse3DI GCAATG 1 cut(s) 1415
BseDI CCNNGG 4 cut(s) 372, 883, 942, 1915
BseGI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1999
BseLI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1702, 2203
BseMI GCAATG 1 cut(s) 1415
BseMII CTCAG 6 cut(s) 110, 236, 1481, 1773, 2100, 2202
BseNI ACTGG 6 cut(s) 604, 1561, 1568, 1689, 2061, 2106
BseRI GAGGAG 4 cut(s) 581, 584, 689, 815
BseX3I CGGCCG 1 cut(s) 361
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 2 cut(s) 773, 2037
Bsh1236I CGCG 1 cut(s) 328
Bsh1285I CGRYCG 2 cut(s) 349, 364
BshFI GGCC 8 cut(s) 39, 363, 623, 680, 956, 1109, 1226, 1567
BsiEI CGRYCG 2 cut(s) 349, 364
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 3 cut(s) 653, 721, 1159
BsiSI CCGG 6 cut(s) 89, 316, 814, 1013, 1917, 2144
BslFI GGGAC 4 cut(s) 461, 636, 1029, 2219
BslI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1702, 2203
BsmAI GTCTC 1 cut(s) 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 4 cut(s) 461, 636, 1029, 2219
BsmI GAATGC 2 cut(s) 439, 1389
BsnI GGCC 8 cut(s) 39, 363, 623, 680, 956, 1109, 1226, 1567
BsoBI CYCGRG 3 cut(s) 653, 721, 1159
Bsp119I TTCGAA 2 cut(s) 576, 1488
Bsp1286I GDGCHC 2 cut(s) 282, 1896
BspACI CCGC 5 cut(s) 349, 671, 1166, 1204, 1985
BspANI GGCC 8 cut(s) 39, 363, 623, 680, 956, 1109, 1226, 1567
BspCNI CTCAG 6 cut(s) 109, 237, 1480, 1774, 2101, 2203
BspFNI CGCG 1 cut(s) 328
BspHI TCATGA 2 cut(s) 267, 1333
BspLI GGNNCC 7 cut(s) 38, 93, 378, 894, 1973, 2207, 2208
BspMAI CTGCAG 2 cut(s) 1304, 1992
BspQI GCTCTTC 2 cut(s) 1103, 1775
BspT104I TTCGAA 2 cut(s) 576, 1488
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 1415
BsrI ACTGG 6 cut(s) 604, 1561, 1568, 1689, 2061, 2106
BssECI CCNNGG 4 cut(s) 372, 883, 942, 1915
BssNAI GTATAC 1 cut(s) 2040
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 3 cut(s) 372, 883, 942
Bst1107I GTATAC 1 cut(s) 2040
Bst4CI ACNGT 7 cut(s) 133, 232, 1258, 1358, 1422, 1546, 2124
Bst6I CTCTTC 7 cut(s) 62, 954, 1103, 1475, 1596, 1775, 2066
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 398
BstBI TTCGAA 2 cut(s) 576, 1488
BstC8I GCNNGC 5 cut(s) 62, 731, 1176, 1263, 1524
BstDEI CTNAG 9 cut(s) 96, 245, 1073, 1431, 1467, 1782, 1941, 2109, 2211
BstF5I GGATG 6 cut(s) 38, 394, 815, 847, 861, 1999
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 5 cut(s) 330, 505, 2034, 2133, 2169
BstMAI GTCTC 1 cut(s) 822
BstMCI CGRYCG 2 cut(s) 349, 364
BstMWI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1405, 1987
BstNSI RCATGY 2 cut(s) 148, 923
BstSCI CCNGG 2 cut(s) 1012, 1915
BstSFI CTRYAG 2 cut(s) 1300, 1988
BstUI CGCG 1 cut(s) 328
BstV2I GAAGAC 3 cut(s) 164, 199, 1497
BstX2I RGATCY 7 cut(s) 121, 376, 892, 1282, 1878, 1971, 2176
BstYI RGATCY 7 cut(s) 121, 376, 892, 1282, 1878, 1971, 2176
BstZ17I GTATAC 1 cut(s) 2040
BstZI CGGCCG 1 cut(s) 361
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 8 cut(s) 39, 363, 623, 680, 956, 1109, 1226, 1567
BtsCI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1999
BtsIMutI CAGTG 6 cut(s) 138, 1279, 1542, 1682, 2054, 2106
Cac8I GCNNGC 5 cut(s) 62, 731, 1176, 1263, 1524
CaiI CAGNNNCTG 1 cut(s) 974
CciI TCATGA 2 cut(s) 267, 1333
CfoI GCGC 5 cut(s) 330, 505, 2034, 2133, 2169
Cfr13I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2206
CseI GACGC 2 cut(s) 375, 2057
Csp6I GTAC 6 cut(s) 217, 658, 1057, 1156, 1512, 1873
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 6 cut(s) 217, 658, 1057, 1156, 1512, 1873
DdeI CTNAG 9 cut(s) 96, 245, 1073, 1431, 1467, 1782, 1941, 2109, 2211
DraIII CACNNNGTG 1 cut(s) 2112
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 2 cut(s) 361, 1565
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 7 cut(s) 62, 954, 1103, 1475, 1596, 1775, 2066
EarI CTCTTC 7 cut(s) 62, 954, 1103, 1475, 1596, 1775, 2066
EciI GGCGGA 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 361
Eco130I CCWWGG 3 cut(s) 372, 883, 942
Eco147I AGGCCT 3 cut(s) 623, 680, 1109
Eco24I GRGCYC 2 cut(s) 282, 1896
Eco47I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2206
Eco47III AGCGCT 1 cut(s) 504
Eco52I CGGCCG 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 86, 1620
Eco88I CYCGRG 3 cut(s) 653, 721, 1159
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 2206
EcoRI GAATTC 2 cut(s) 72, 1295
EcoT14I CCWWGG 3 cut(s) 372, 883, 942
EcoT38I GRGCYC 2 cut(s) 282, 1896
ErhI CCWWGG 3 cut(s) 372, 883, 942
Esp3I CGTCTC 1 cut(s) 822
FalI AAGNNNNNCTT 4 cut(s) 1224, 1256, 1482, 1514
FaqI GGGAC 4 cut(s) 461, 636, 1029, 2219
FblI GTMKAC 3 cut(s) 227, 2022, 2039
FokI GGATG 6 cut(s) 45, 401, 822, 854, 868, 2006
FriOI GRGCYC 2 cut(s) 282, 1896
FspBI CTAG 3 cut(s) 444, 852, 1019
GlaI GCGC 5 cut(s) 329, 504, 2033, 2132, 2168
GsaI CCCAGC 1 cut(s) 1155
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 8 cut(s) 39, 363, 623, 680, 956, 1109, 1226, 1567
HapII CCGG 6 cut(s) 89, 316, 814, 1013, 1917, 2144
HgaI GACGC 2 cut(s) 375, 2057
HhaI GCGC 5 cut(s) 330, 505, 2034, 2133, 2169
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 5 cut(s) 328, 503, 2032, 2131, 2167
HinP1I GCGC 5 cut(s) 328, 503, 2032, 2131, 2167
HincII GTYRAC 3 cut(s) 228, 1743, 2023
HindII GTYRAC 3 cut(s) 228, 1743, 2023
HindIII AAGCTT 2 cut(s) 1520, 1664
HinfI GANTC 7 cut(s) 224, 449, 644, 803, 1810, 2074, 2215
HpaII CCGG 6 cut(s) 89, 316, 814, 1013, 1917, 2144
HphI GGTGA 9 cut(s) 568, 1019, 1223, 1417, 1583, 1931, 1939, 2071, 2176
Hpy166II GTNNAC 9 cut(s) 228, 421, 782, 1425, 1743, 1840, 1947, 2023, 2040
Hpy188I TCNGA 7 cut(s) 156, 178, 287, 456, 1585, 1783, 1801
Hpy8I GTNNAC 9 cut(s) 228, 421, 782, 1425, 1743, 1840, 1947, 2023, 2040
Hpy99I CGWCG 6 cut(s) 206, 215, 232, 263, 362, 369
HpyCH4III ACNGT 7 cut(s) 133, 232, 1258, 1358, 1422, 1546, 2124
HpyCH4IV ACGT 6 cut(s) 204, 357, 417, 478, 516, 2135
HpyCH4V TGCA 9 cut(s) 437, 754, 977, 1265, 1302, 1617, 1990, 2054, 2128
HpyF10VI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1405, 1987
HpyF3I CTNAG 9 cut(s) 96, 245, 1073, 1431, 1467, 1782, 1941, 2109, 2211
HpySE526I ACGT 6 cut(s) 204, 357, 417, 478, 516, 2135
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 5 cut(s) 328, 503, 2032, 2131, 2167
KflI GGGWCCC 1 cut(s) 2206
LguI GCTCTTC 2 cut(s) 1103, 1775
LmnI GCTCC 3 cut(s) 91, 374, 487
LweI GCATC 3 cut(s) 261, 379, 800
MaeI CTAG 3 cut(s) 444, 852, 1019
MaeII ACGT 6 cut(s) 204, 357, 417, 478, 516, 2135
MfeI CAATTG 2 cut(s) 1690, 2043
MflI RGATCY 7 cut(s) 121, 376, 892, 1282, 1878, 1971, 2176
MhlI GDGCHC 2 cut(s) 282, 1896
MlsI TGGCCA 1 cut(s) 1567
MluNI TGGCCA 1 cut(s) 1567
MlyI GAGTC 5 cut(s) 233, 443, 812, 1804, 2083
MmeI TCCRAC 5 cut(s) 520, 723, 870, 973, 1509
Mox20I TGGCCA 1 cut(s) 1567
MroXI GAANNNNTTC 2 cut(s) 1484, 1666
MscI TGGCCA 1 cut(s) 1567
MseI TTAA 5 cut(s) 192, 399, 408, 912, 1817
MslI CAYNNNNRTG 3 cut(s) 1622, 1730, 1928
Msp20I TGGCCA 1 cut(s) 1567
MspA1I CMGCKG 3 cut(s) 787, 974, 1987
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 6 cut(s) 89, 316, 814, 1013, 1917, 2144
MspR9I CCNGG 2 cut(s) 1014, 1917
MunI CAATTG 2 cut(s) 1690, 2043
Mva1269I GAATGC 2 cut(s) 439, 1389
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1405, 1987
NciI CCSGG 2 cut(s) 1014, 1917
NlaIV GGNNCC 7 cut(s) 38, 93, 378, 894, 1973, 2207, 2208
NmuCI GTSAC 8 cut(s) 1217, 1274, 1447, 1540, 1919, 2008, 2104, 2189
NspI RCATGY 2 cut(s) 148, 923
NspV TTCGAA 2 cut(s) 576, 1488
PaeR7I CTCGAG 1 cut(s) 1159
PagI TCATGA 2 cut(s) 267, 1333
PceI AGGCCT 3 cut(s) 623, 680, 1109
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 2 cut(s) 1103, 1775
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 219, 423
PctI GAATGC 2 cut(s) 439, 1389
PdmI GAANNNNTTC 2 cut(s) 1484, 1666
PfeI GAWTC 2 cut(s) 644, 2215
PflMI CCANNNNNTGG 1 cut(s) 889
PleI GAGTC 5 cut(s) 232, 443, 811, 1804, 2082
PpsI GAGTC 5 cut(s) 232, 443, 811, 1804, 2082
PpuMI RGGWCCY 1 cut(s) 2206
PscI ACATGT 1 cut(s) 144
PshBI ATTAAT 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 2206
PspFI CCCAGC 1 cut(s) 1151
PspN4I GGNNCC 7 cut(s) 38, 93, 378, 894, 1973, 2207, 2208
PspPI GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2206
PspPPI RGGWCCY 1 cut(s) 2206
PspXI VCTCGAGB 1 cut(s) 1159
PstI CTGCAG 2 cut(s) 1304, 1992
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 7 cut(s) 121, 376, 892, 1282, 1878, 1971, 2176
PvuII CAGCTG 2 cut(s) 787, 974
RsaI GTAC 6 cut(s) 218, 659, 1058, 1157, 1513, 1874
RsaNI GTAC 6 cut(s) 217, 658, 1057, 1156, 1512, 1873
RseI CAYNNNNRTG 3 cut(s) 1622, 1730, 1928
SacI GAGCTC 1 cut(s) 282
SalI GTCGAC 2 cut(s) 226, 2021
SapI GCTCTTC 2 cut(s) 1103, 1775
SaqAI TTAA 5 cut(s) 192, 399, 408, 912, 1817
Sau96I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2206
SchI GAGTC 5 cut(s) 233, 443, 812, 1804, 2083
ScrFI CCNGG 2 cut(s) 1014, 1917
SduI GDGCHC 2 cut(s) 282, 1896
SfaNI GCATC 3 cut(s) 261, 379, 800
SfcI CTRYAG 2 cut(s) 1300, 1988
Sfr274I CTCGAG 1 cut(s) 1159
SfuI TTCGAA 2 cut(s) 576, 1488
SinI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2206
SlaI CTCGAG 1 cut(s) 1159
SmiMI CAYNNNNRTG 3 cut(s) 1622, 1730, 1928
SmlI CTYRAG 7 cut(s) 383, 398, 682, 1040, 1159, 1199, 2198
SmoI CTYRAG 7 cut(s) 383, 398, 682, 1040, 1159, 1199, 2198
SpeI ACTAGT 1 cut(s) 1018
SseBI AGGCCT 3 cut(s) 623, 680, 1109
SsiI CCGC 5 cut(s) 349, 671, 1166, 1204, 1985
SspI AATATT 2 cut(s) 1637, 2085
SspMI CTAG 3 cut(s) 444, 852, 1019
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 3 cut(s) 623, 680, 1109
StyD4I CCNGG 2 cut(s) 1012, 1915
StyI CCWWGG 3 cut(s) 372, 883, 942
TaaI ACNGT 7 cut(s) 133, 232, 1258, 1358, 1422, 1546, 2124
TaiI ACGT 6 cut(s) 207, 360, 420, 481, 519, 2138
TatI WGTACW 4 cut(s) 216, 657, 1056, 1872
TfiI GAWTC 2 cut(s) 644, 2215
Tru1I TTAA 5 cut(s) 192, 399, 408, 912, 1817
Tru9I TTAA 5 cut(s) 192, 399, 408, 912, 1817
TscAI CASTG 6 cut(s) 138, 1279, 1549, 1689, 2061, 2113
TseFI GTSAC 8 cut(s) 1217, 1274, 1447, 1540, 1919, 2008, 2104, 2189
Tsp45I GTSAC 8 cut(s) 1217, 1274, 1447, 1540, 1919, 2008, 2104, 2189
TspGWI ACGGA 2 cut(s) 1528, 2027
TspRI CASTG 6 cut(s) 138, 1279, 1549, 1689, 2061, 2113
Van91I CCANNNNNTGG 1 cut(s) 889
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2206
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 2 cut(s) 72, 1295
XceI RCATGY 2 cut(s) 148, 923
XhoI CTCGAG 1 cut(s) 1159
XmiI GTMKAC 3 cut(s) 227, 2022, 2039
XmnI GAANNNNTTC 2 cut(s) 1484, 1666
XspI CTAG 3 cut(s) 444, 852, 1019
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.