Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0000162

Physical Location & Seq

Genomic Coordinates Forward (+)

10445756 .. 10447485

Gene Structure 1730 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1135800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 909 bp

ATGGAAGGCAGAAGACCAGTTATTAATTGGGGCGACGGATGGAACTACATCCAGGAGAGGATTACGAAACTAATTAGGGTAATTACAGAAGGGTTACCGGAGAATCATATCGATGCAGAAGAATGCATGGCTGCTTATACCATGATCTTTAACATGTGCATTCAGAAACCTCCTTATGACTATTCTCCTAATCTTTACGAAAAATATCGGGAGACATTCGAGGAATACCTTACTTCAATGGTGCTGCCAGTTCTAAGAGAGAGGCCAGATGAGTTTTTACTGCATGATTTTGTCAAACGTTGGGAAAACCACAAAGTCATCGTTAGGTGGCTGTCACGCTTGTTTATCTATCTTGATCGTTACTACATTGAGCGGAGATCACTTCCTGGGCTGAAAGAAGTTGCACTTACCTGTTTCCGCGACTTGGTTTACCTCCAGGTGAATGCGCGCGTGACAGCAGCTGTAATAGGTTTTATTCATAGAGAACGTGAGGGAGCGCAAATTGACAGAGCGCTGCTGAAGAATGTGATGGATACATTGGTTGAAATCAAGGTGGAGGGAGTGAAGACTGCTTATGAAGTGAACTTTGAAGCACACATGCTGAGAGAAACCGGTGTATACTATTCGCATAAAGCATCGAGTTGGATCATGGAGGATTCTTATAGCGATTACATGTTGAAGGCGGAGGAATGCGTGAGAAGGGAGAGGGAGAGGGTTTCGCATTACCTGAATCCAAGTAGTGAAAATAAGGTGGTGGAGATTGTGAAACATTGCTTGCTGGTGGTTTATGCAACTCAACTGATTGAAAAGAAGCTTTTTTCTGAATCTGTATCTAGTGCTTCGCTTGCAGCTGATTATGTTGAGGAGCTTTCAAGGAAATTTATTGCTAGTGTAACATTGGCACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000228 GO:0000793 GO:0000794 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009524 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009987 GO:0010033 GO:0010087 GO:0010154 GO:0015630 GO:0019899 GO:0022414 GO:0023052 GO:0031461 GO:0031625 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0042221 GO:0042752 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0099402 GO:1901700 GO:1901701 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04114 ko04120 ko04141 ko04310 ko04340 ko04341 ko04350 ko04710 ko05168 ko05200 map04110 map04111 map04114 map04120 map04141 map04310 map04340 map04341 map04350 map04710 map05168 map05200

Pfam Domains

Protein Families

Cullin Cullin_Nedd8

Protein Analysis

303

Amino Acids

35.59

Weight (kDa)

5.98

Isoelectric Point (pI)

53.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cullin	PF00888	14 - 278	2.9e-52	Cullin alpha solenoid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca	FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica	MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica	Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis	pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis	RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata	RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora	Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii	Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa	Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha	Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana	Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	373
AccI	GTMKAC	1 cut(s)	618
AccII	CGCG	3 cut(s)	420, 448, 450
AciI	CCGC	3 cut(s)	373, 418, 683
AclI	AACGTT	1 cut(s)	298
AclWI	GGATC	1 cut(s)	653
AcsI	RAATTY	1 cut(s)	878
AcuI	CTGAAG	1 cut(s)	539
AfeI	AGCGCT	1 cut(s)	513
AfiI	CCNNNNNNNGG	1 cut(s)	424
AflIII	ACRYGT	2 cut(s)	153, 672
AgeI	ACCGGT	1 cut(s)	611
AgsI	TTSAA	6 cut(s)	237, 545, 590, 679, 806, 873
AjnI	CCWGG	3 cut(s)	51, 385, 435
AluBI	AGCT	4 cut(s)	461, 814, 851, 868
AluI	AGCT	4 cut(s)	461, 814, 851, 868
Alw26I	GTCTC	1 cut(s)	206
AlwI	GGATC	1 cut(s)	653
AlwNI	CAGNNNCTG	1 cut(s)	461
Aor51HI	AGCGCT	1 cut(s)	513
AoxI	GGCC	1 cut(s)	263
ApeKI	GCWGC	5 cut(s)	131, 244, 458, 514, 848
ApoI	RAATTY	1 cut(s)	878
AseI	ATTAAT	1 cut(s)	24
AsiGI	ACCGGT	1 cut(s)	611
AspLEI	GCGC	4 cut(s)	448, 450, 499, 514
AsuHPI	GGTGA	1 cut(s)	451
BaeI	ACNNNNGTAYC	2 cut(s)	525, 558
BbsI	GAAGAC	2 cut(s)	19, 572
BbvI	GCAGC	5 cut(s)	118, 231, 470, 501, 860
BccI	CCATC	2 cut(s)	33, 523
BciT130I	CCWGG	3 cut(s)	53, 387, 437
BciVI	GTATCC	1 cut(s)	526
BcoDI	GTCTC	1 cut(s)	206
BfaI	CTAG	2 cut(s)	834, 888
BfoI	RGCGCY	1 cut(s)	515
BfuI	GTATCC	1 cut(s)	526
BisI	GCNGC	5 cut(s)	132, 245, 459, 515, 849
BlsI	GCNGC	5 cut(s)	133, 246, 460, 516, 850
Bme1390I	CCNGG	3 cut(s)	53, 387, 437
BmrFI	CCNGG	3 cut(s)	53, 387, 437
BmsI	GCATC	2 cut(s)	103, 644
BpiI	GAAGAC	2 cut(s)	19, 572
BpmI	CTGGAG	1 cut(s)	419
Bsa29I	ATCGAT	1 cut(s)	111
BsaJI	CCNNGG	1 cut(s)	386
BsaWI	WCCGGW	2 cut(s)	97, 611
Bsc4I	CCNNNNNNNGG	1 cut(s)	424
Bse118I	RCCGGY	1 cut(s)	611
Bse1I	ACTGG	2 cut(s)	17, 248
Bse3DI	GCAATG	1 cut(s)	769
BseBI	CCWGG	3 cut(s)	53, 387, 437
BseCI	ATCGAT	1 cut(s)	111
BseDI	CCNNGG	1 cut(s)	386
BseGI	GGATG	2 cut(s)	44, 48
BseLI	CCNNNNNNNGG	1 cut(s)	424
BseMI	GCAATG	1 cut(s)	769
BseMII	CTCAG	1 cut(s)	593
BseNI	ACTGG	2 cut(s)	17, 248
BsePI	GCGCGC	1 cut(s)	446
BseRI	GAGGAG	1 cut(s)	878
BseXI	GCAGC	5 cut(s)	118, 231, 470, 501, 860
Bsh1236I	CGCG	3 cut(s)	420, 448, 450
BshFI	GGCC	1 cut(s)	265
BshTI	ACCGGT	1 cut(s)	611
BshVI	ATCGAT	1 cut(s)	111
BsiSI	CCGG	2 cut(s)	98, 612
BslI	CCNNNNNNNGG	1 cut(s)	424
BsmAI	GTCTC	1 cut(s)	206
BsmI	GAATGC	4 cut(s)	128, 159, 448, 695
BsnI	GGCC	1 cut(s)	265
Bsp143I	GATC	4 cut(s)	144, 355, 377, 645
BspACI	CCGC	3 cut(s)	373, 418, 683
BspANI	GGCC	1 cut(s)	265
BspCNI	CTCAG	1 cut(s)	594
BspDI	ATCGAT	1 cut(s)	111
BspFNI	CGCG	3 cut(s)	420, 448, 450
BspPI	GGATC	1 cut(s)	653
BsrBI	CCGCTC	1 cut(s)	373
BsrDI	GCAATG	1 cut(s)	769
BsrFI	RCCGGY	1 cut(s)	611
BsrI	ACTGG	2 cut(s)	17, 248
BssAI	RCCGGY	1 cut(s)	611
BssECI	CCNNGG	1 cut(s)	386
BssHII	GCGCGC	1 cut(s)	446
BssMI	GATC	4 cut(s)	144, 355, 377, 645
BssNAI	GTATAC	1 cut(s)	619
Bst1107I	GTATAC	1 cut(s)	619
Bst2UI	CCWGG	3 cut(s)	53, 387, 437
Bst4CI	ACNGT	1 cut(s)	906
BstC8I	GCNNGC	3 cut(s)	448, 776, 846
BstDEI	CTNAG	2 cut(s)	254, 602
BstEII	GGTNACC	1 cut(s)	93
BstF5I	GGATG	2 cut(s)	44, 48
BstFNI	CGCG	3 cut(s)	420, 448, 450
BstH2I	RGCGCY	1 cut(s)	515
BstHHI	GCGC	4 cut(s)	448, 450, 499, 514
BstKTI	GATC	4 cut(s)	147, 358, 380, 648
BstMAI	GTCTC	1 cut(s)	206
BstMBI	GATC	4 cut(s)	144, 355, 377, 645
BstMWI	GCNNNNNNNGC	1 cut(s)	845
BstNI	CCWGG	3 cut(s)	53, 387, 437
BstNSI	RCATGY	3 cut(s)	157, 601, 676
BstPI	GGTNACC	1 cut(s)	93
BstSCI	CCNGG	3 cut(s)	51, 385, 435
BstUI	CGCG	3 cut(s)	420, 448, 450
BstV1I	GCAGC	5 cut(s)	118, 231, 470, 501, 860
BstV2I	GAAGAC	2 cut(s)	19, 572
BstZ17I	GTATAC	1 cut(s)	619
Bsu15I	ATCGAT	1 cut(s)	111
BsuI	GTATCC	1 cut(s)	526
BsuRI	GGCC	1 cut(s)	265
BsuTUI	ATCGAT	1 cut(s)	111
BtsCI	GGATG	2 cut(s)	44, 48
Cac8I	GCNNGC	3 cut(s)	448, 776, 846
CaiI	CAGNNNCTG	1 cut(s)	461
CfoI	GCGC	4 cut(s)	448, 450, 499, 514
Cfr10I	RCCGGY	1 cut(s)	611
ClaI	ATCGAT	1 cut(s)	111
CspAI	ACCGGT	1 cut(s)	611
CviAII	CATG	7 cut(s)	127, 142, 154, 284, 598, 649, 673
CviJI	RGCY	8 cut(s)	131, 265, 331, 391, 461, 814, 851, 868
CviKI_1	RGCY	8 cut(s)	131, 265, 331, 391, 461, 814, 851, 868
DdeI	CTNAG	2 cut(s)	254, 602
DpnI	GATC	4 cut(s)	146, 357, 379, 647
DpnII	GATC	4 cut(s)	144, 355, 377, 645
EciI	GGCGGA	1 cut(s)	698
Eco47III	AGCGCT	1 cut(s)	513
Eco57I	CTGAAG	1 cut(s)	539
Eco91I	GGTNACC	1 cut(s)	93
EcoO65I	GGTNACC	1 cut(s)	93
EcoRII	CCWGG	3 cut(s)	51, 385, 435
EcoT22I	ATGCAT	1 cut(s)	128
FaeI	CATG	7 cut(s)	130, 145, 157, 287, 601, 652, 676
FalI	AAGNNNNNCTT	2 cut(s)	390, 422
FatI	CATG	7 cut(s)	126, 141, 153, 283, 597, 648, 672
FblI	GTMKAC	1 cut(s)	618
Fnu4HI	GCNGC	5 cut(s)	132, 245, 459, 515, 849
FokI	GGATG	2 cut(s)	35, 51
Fsp4HI	GCNGC	5 cut(s)	132, 245, 459, 515, 849
FspBI	CTAG	2 cut(s)	834, 888
GlaI	GCGC	4 cut(s)	447, 449, 498, 513
GluI	GCNGC	5 cut(s)	132, 245, 459, 515, 849
GsuI	CTGGAG	1 cut(s)	419
HaeII	RGCGCY	1 cut(s)	515
HaeIII	GGCC	1 cut(s)	265
HapII	CCGG	2 cut(s)	98, 612
HhaI	GCGC	4 cut(s)	448, 450, 499, 514
Hin1II	CATG	7 cut(s)	130, 145, 157, 287, 601, 652, 676
Hin6I	GCGC	4 cut(s)	446, 448, 497, 512
HinP1I	GCGC	4 cut(s)	446, 448, 497, 512
HindIII	AAGCTT	1 cut(s)	812
HinfI	GANTC	4 cut(s)	103, 656, 730, 824
HpaII	CCGG	2 cut(s)	98, 612
HphI	GGTGA	1 cut(s)	451
Hpy166II	GTNNAC	3 cut(s)	430, 583, 619
Hpy188I	TCNGA	2 cut(s)	165, 823
Hpy188III	TCNNGA	2 cut(s)	209, 353
Hpy8I	GTNNAC	3 cut(s)	430, 583, 619
Hpy99I	CGWCG	1 cut(s)	38
HpyAV	CCTTC	3 cut(s)	83, 673, 693
HpyCH4III	ACNGT	1 cut(s)	906
HpyCH4IV	ACGT	2 cut(s)	298, 487
HpyCH4V	TGCA	7 cut(s)	116, 126, 159, 283, 404, 791, 848
HpyF10VI	GCNNNNNNNGC	1 cut(s)	845
HpyF3I	CTNAG	2 cut(s)	254, 602
HpySE526I	ACGT	2 cut(s)	298, 487
Hsp92II	CATG	7 cut(s)	130, 145, 157, 287, 601, 652, 676
HspAI	GCGC	4 cut(s)	446, 448, 497, 512
Kzo9I	GATC	4 cut(s)	144, 355, 377, 645
LmnI	GCTCC	2 cut(s)	494, 865
Lsp1109I	GCAGC	5 cut(s)	118, 231, 470, 501, 860
LweI	GCATC	2 cut(s)	103, 644
MaeI	CTAG	2 cut(s)	834, 888
MaeII	ACGT	2 cut(s)	298, 487
MaeIII	GTNAC	5 cut(s)	93, 333, 359, 451, 892
MalI	GATC	4 cut(s)	146, 357, 379, 647
MbiI	CCGCTC	1 cut(s)	373
MboI	GATC	4 cut(s)	144, 355, 377, 645
MboII	GAAGA	4 cut(s)	24, 131, 532, 577
MluCI	AATT	5 cut(s)	25, 72, 81, 501, 878
MmeI	TCCRAC	1 cut(s)	623
Mph1103I	ATGCAT	1 cut(s)	128
MseI	TTAA	2 cut(s)	24, 150
MslI	CAYNNNNRTG	1 cut(s)	111
MspA1I	CMGCKG	2 cut(s)	461, 851
MspI	CCGG	2 cut(s)	98, 612
MspR9I	CCNGG	3 cut(s)	53, 387, 437
Mva1269I	GAATGC	4 cut(s)	128, 159, 448, 695
MvaI	CCWGG	3 cut(s)	53, 387, 437
MvnI	CGCG	3 cut(s)	420, 448, 450
MwoI	GCNNNNNNNGC	1 cut(s)	845
NdeII	GATC	4 cut(s)	144, 355, 377, 645
NlaIII	CATG	7 cut(s)	130, 145, 157, 287, 601, 652, 676
NmuCI	GTSAC	2 cut(s)	333, 451
NsiI	ATGCAT	1 cut(s)	128
NspI	RCATGY	3 cut(s)	157, 601, 676
PauI	GCGCGC	1 cut(s)	446
PciI	ACATGT	2 cut(s)	153, 672
PctI	GAATGC	4 cut(s)	128, 159, 448, 695
PfeI	GAWTC	4 cut(s)	103, 656, 730, 824
PfoI	TCCNGGA	1 cut(s)	51
PinAI	ACCGGT	1 cut(s)	611
PkrI	GCNGC	5 cut(s)	133, 246, 460, 516, 850
PscI	ACATGT	2 cut(s)	153, 672
PshBI	ATTAAT	1 cut(s)	24
Psp1406I	AACGTT	1 cut(s)	298
Psp6I	CCWGG	3 cut(s)	51, 385, 435
PspEI	GGTNACC	1 cut(s)	93
PspGI	CCWGG	3 cut(s)	51, 385, 435
PstNI	CAGNNNCTG	1 cut(s)	461
PteI	GCGCGC	1 cut(s)	446
PvuII	CAGCTG	2 cut(s)	461, 851
RseI	CAYNNNNRTG	1 cut(s)	111
SaqAI	TTAA	2 cut(s)	24, 150
SatI	GCNGC	5 cut(s)	132, 245, 459, 515, 849
Sau3AI	GATC	4 cut(s)	144, 355, 377, 645
ScrFI	CCNGG	3 cut(s)	53, 387, 437
SfaNI	GCATC	2 cut(s)	103, 644
SmiMI	CAYNNNNRTG	1 cut(s)	111
Sse9I	AATT	5 cut(s)	25, 72, 81, 501, 878
SsiI	CCGC	3 cut(s)	373, 418, 683
SspMI	CTAG	2 cut(s)	834, 888
StyD4I	CCNGG	3 cut(s)	51, 385, 435
TaaI	ACNGT	1 cut(s)	906
TaiI	ACGT	2 cut(s)	301, 490
TaqI	TCGA	3 cut(s)	111, 219, 638
TasI	AATT	5 cut(s)	25, 72, 81, 501, 878
TfiI	GAWTC	4 cut(s)	103, 656, 730, 824
Tru1I	TTAA	2 cut(s)	24, 150
Tru9I	TTAA	2 cut(s)	24, 150
TseFI	GTSAC	2 cut(s)	333, 451
TseI	GCWGC	5 cut(s)	131, 244, 458, 514, 848
Tsp45I	GTSAC	2 cut(s)	333, 451
TspDTI	ATGAA	2 cut(s)	467, 591
TspGWI	ACGGA	1 cut(s)	51
VspI	ATTAAT	1 cut(s)	24
XapI	RAATTY	1 cut(s)	878
XceI	RCATGY	3 cut(s)	157, 601, 676
XcmI	CCANNNNNNNNNTGG	1 cut(s)	24
XmiI	GTMKAC	1 cut(s)	618
XspI	CTAG	2 cut(s)	834, 888
Zsp2I	ATGCAT	1 cut(s)	128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.