FvH4_3g22682

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000162
Physical Location & Seq
Forward (+)
15711712 .. 15713774
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22682.t1

Sequence Viewer

Length: 2034 bp
ATGGAGCAGAAACAATGCATCCAGTTTCAAGAAGGATGGGACTATATGGAGAAGGGGATCACCAAGCTGAAGAGAATTCTCGAAGGAGAATCGGAGCAGTTCAGATCAGATGAATACATGCATCTTTACACCAAGATCTACAACATGTGTACTCAGAAGCCCCCTCACGACTACTCTAAACTGCTCTATGAGGGATATGAAGGGGTCTTCGTCGAGTACACCGAGTCGACGGTGTTGCCTACTCTGAGAGAAAAGCACGGCGAGTTCATGCTACGTGAGTTTGTTCAAAGATGGTCCAATCATAAACTCATGGTCAGGTGGCTCTCGCGCTTCTTTCACTATCTCGACCGGTATTTCATCGCTAGGCGTAACCTCGATTCGCTCAAGGTCGCTGGACATAAGAAGTTTAAGGAGCATGCTTATATGGAGATGAAAGAAAAGGCTAAGGACTTTCTGATAGCTCTTATTTTGATGGAACGTGAAGGGGAGCAGATTGACAGAGGGCTGATGAAGAATGTGGTTGATATATTCGTGGAAATTGGAATGGGAGAGATGATCAACTACGAGGAGGATTTCGAAAAGCACATGGTTGAGGATTCCGGAGAGTACTACTCTCGAAAGGCCTCGTACATGGTGAAGGCGGAGGAGGCATTGAGGAAGGAGAAGGAGAGAGTTTCGCATTACCTGCACTCGGGCAGCGAGCAAAAGCTGTTGGAGAGGGTGCAGAAGGAGTTGCTGGTTGTTCCAGGTCCACAGCTGCTTGACAAAGAGGAGTCTGGGTGCCGAGCCTTGCTTCGAGACGATAGGGAGGATGATTTGTCTAGGATGTACAGGCTTTACCATAAAATACCACAAGGGTTGGAACCTGTTGCGGCTCTGTTTAAGAATCATGTTACTAATGAAGGCACGGCCTTGGTGCAACAGGCTGAAGAGGCGACGGCAGCTGCAACTGGCACCAAGCCAGGTGGTGGTGCTGGTGAGCCGGGGCTAGTGTTTATTAAGAAAGTACTTGAGCTACATGATAAGTACATGGGATATGTACTTAACTGCTTCCAGAACCACGCGCTCTTCCACAAGGCCTTGAAAGAGGCTTTTGAGGTGTTCTGTAATAAATCTATGGCTGGGTACTCGAGTGCGGAATTGCTAGCTACATTCTGTGATAGCCTCCTCAAGAAGAATGCCGAAAGTGTAGAGGCCATGGAAGAAACACTTGACAAGGTTGTTACGGTGCTTGCATATTTCAGTGACAAAGATCTTTTCGCCGAATTTTGCAGGAAGAAACTTGGCCGTAGGCTGCTTACTGGTGATGACCCCCATCATGATGATGAGAGAATGCTTCTAGCAAAGCTGAAGCAACAATTTGGTGCACAGTTCACCTGCAAGATGGAGGGAATGGTCACTGATATTACCGTTTCTCGGGATAAACTGAGGGCTTTCCAAGACTTGGGTTGGAGTTCAAAGCTTGGGTTGGATTTCTCAGTCACTGTTCTAACCACTGGTAATTGGCCAACCTACAAAACCTCTGATATTCACCTTCCTGAAGAGATGGTCAAGTGTGTAGAAGTGTTCAAACAATACTATGACACACAACATAAACAGAGAAAACTTTCATGGATCTATACATTGGGTAATTGCTCTGTCAGGGGCAACTTTGAGCCAAAATCTATTGAGTTGATTATGTCAGCATATCAAGCTGCTCTGCTCCTGCTCTTCAACAACTCGGAGACATTGAGCTTTTCAGAGATAGTGACTCAGTTGAATCTTACCAAAGATGAAGTGCACAGAACGCTTCATTCGGTTTCGTGTGCCAAGTACAAGATCCTTGTGAAGGAGCCTAACACAAAGACAATCTCCCAGAGTGACACTTTTACCTTCAACTCCAAGTTCACCGATAGACTGAGGAAAATCAGGGTCACCCCCGTGCCGCCGCCGCAGGATGAGAGGAAGAAAGTGACAGAAGATGTCGACAAAGAGCGCAAGTATACCATTGATGCTGCACTTGTGAGGATTATGAAGAGGGTCATCAACAACTGA

Protein Analysis

678

Amino Acids

78.71

Weight (kDa)

6.89

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 13 - 474 2.2e-111 Cullin alpha solenoid domain
Cullin_AB PF26557 496 - 629 9.6e-44 Cullin alpha+beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1385
Acc36I ACCTGC 2 cut(s) 693, 1385
AccB1I GGYRCC 2 cut(s) 780, 953
AccB7I CCANNNNNTGG 2 cut(s) 968, 1444
AccI GTMKAC 3 cut(s) 227, 1965, 1982
AccII CGCG 2 cut(s) 328, 1064
AccIII TCCGGA 1 cut(s) 599
AciI CCGC 6 cut(s) 641, 872, 1136, 1925, 1928, 1931
AclWI GGATC 3 cut(s) 65, 1622, 1813
AcoI YGGCCR 2 cut(s) 1285, 1505
AcsI RAATTY 2 cut(s) 75, 1265
AcuI CTGAAG 4 cut(s) 89, 948, 1370, 1560
AfiI CCNNNNNNNGG 5 cut(s) 691, 968, 1416, 1444, 1828
AflIII ACRYGT 1 cut(s) 144
AgeI ACCGGT 1 cut(s) 348
AgsI TTSAA 8 cut(s) 29, 287, 1084, 1458, 1570, 1714, 1759, 1876
AjnI CCWGG 2 cut(s) 745, 961
AleI CACNNNNGTG 1 cut(s) 1919
Alw21I GWGCWC 2 cut(s) 1369, 1782
Alw26I GTCTC 2 cut(s) 792, 1718
Alw44I GTGCAC 2 cut(s) 1365, 1778
AlwI GGATC 3 cut(s) 65, 1622, 1813
AlwNI CAGNNNCTG 1 cut(s) 1484
Ama87I CYCGRG 3 cut(s) 691, 1129, 1416
Aor13HI TCCGGA 1 cut(s) 599
AoxI GGCC 6 cut(s) 621, 909, 1077, 1194, 1285, 1505
ApaLI GTGCAC 2 cut(s) 1365, 1778
ApeKI GCWGC 7 cut(s) 696, 757, 941, 944, 1294, 1694, 1994
ApoI RAATTY 2 cut(s) 75, 1265
AsiGI ACCGGT 1 cut(s) 348
Asp700I GAANNNNTTC 1 cut(s) 1606
AspLEI GCGC 3 cut(s) 330, 1066, 1977
AspS9I GGNCC 2 cut(s) 294, 749
AsuC2I CCSGG 1 cut(s) 984
AsuHPI GGTGA 8 cut(s) 52, 646, 989, 1316, 1366, 1523, 1879, 1906
AsuII TTCGAA 1 cut(s) 576
AsuNHI GCTAGC 1 cut(s) 1144
AvaI CYCGRG 3 cut(s) 691, 1129, 1416
AvaII GGWCC 2 cut(s) 294, 749
BaeGI GKGCMC 2 cut(s) 1369, 1782
BaeI ACNNNNGTAYC 2 cut(s) 1117, 1150
BalI TGGCCA 1 cut(s) 1507
BanI GGYRCC 2 cut(s) 780, 953
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 2 cut(s) 1369, 1782
BbvI GCAGC 7 cut(s) 708, 744, 931, 953, 1281, 1681, 1981
BccI CCATC 6 cut(s) 30, 285, 466, 1323, 1378, 1540
BceAI ACGGC 4 cut(s) 274, 924, 954, 1272
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 747, 963
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 1 cut(s) 984
BcoDI GTCTC 2 cut(s) 792, 1718
BfaI CTAG 5 cut(s) 363, 822, 989, 1145, 1340
BfuAI ACCTGC 2 cut(s) 693, 1385
BglII AGATCT 2 cut(s) 135, 1252
BmcAI AGTACT 2 cut(s) 608, 1008
Bme1390I CCNGG 3 cut(s) 747, 963, 984
Bme18I GGWCC 2 cut(s) 294, 749
BmeT110I CYCGRG 3 cut(s) 691, 1129, 1416
BmgT120I GGNCC 2 cut(s) 294, 749
BmiI GGNNCC 4 cut(s) 782, 864, 955, 1833
BmrFI CCNGG 3 cut(s) 747, 963, 984
BmsI GCATC 3 cut(s) 27, 130, 1981
BmtI GCTAGC 1 cut(s) 1148
BpiI GAAGAC 1 cut(s) 199
BplI GAGNNNNNCTC 2 cut(s) 596, 628
Bpu10I CCTNAGC 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 576
BpuEI CTTGAG 3 cut(s) 368, 1031, 1154
BpuMI CCSGG 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 275
BsaJI CCNNGG 3 cut(s) 912, 983, 1197
BsaWI WCCGGW 2 cut(s) 348, 599
BsaXI ACNNNNNCTCC 2 cut(s) 764, 794
Bsc4I CCNNNNNNNGG 5 cut(s) 691, 968, 1416, 1444, 1828
Bse118I RCCGGY 1 cut(s) 348
Bse1I ACTGG 4 cut(s) 22, 955, 1306, 1501
BseAI TCCGGA 1 cut(s) 599
BseBI CCWGG 2 cut(s) 747, 963
BseDI CCNNGG 3 cut(s) 912, 983, 1197
BseGI GGATG 5 cut(s) 18, 41, 817, 831, 1942
BseLI CCNNNNNNNGG 5 cut(s) 691, 968, 1416, 1444, 1828
BseMII CTCAG 6 cut(s) 167, 236, 1418, 1491, 1766, 1889
BseNI ACTGG 4 cut(s) 22, 955, 1306, 1501
BseRI GAGGAG 4 cut(s) 581, 659, 785, 1157
BseSI GKGCMC 2 cut(s) 1369, 1782
BseXI GCAGC 7 cut(s) 708, 744, 931, 953, 1281, 1681, 1981
BseYI CCCAGC 1 cut(s) 1121
BsgI GTGCAG 3 cut(s) 671, 743, 1980
Bsh1236I CGCG 2 cut(s) 328, 1064
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 6 cut(s) 623, 911, 1079, 1196, 1287, 1507
BshNI GGYRCC 2 cut(s) 780, 953
BshTI ACCGGT 1 cut(s) 348
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 1369, 1782
BsiHKCI CYCGRG 3 cut(s) 691, 1129, 1416
BsiSI CCGG 3 cut(s) 349, 600, 983
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 5 cut(s) 691, 968, 1416, 1444, 1828
BsmAI GTCTC 2 cut(s) 792, 1718
BsmBI CGTCTC 1 cut(s) 792
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 2 cut(s) 1183, 1338
BsnI GGCC 6 cut(s) 623, 911, 1079, 1196, 1287, 1507
BsoBI CYCGRG 3 cut(s) 691, 1129, 1416
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 1369, 1782
Bsp13I TCCGGA 1 cut(s) 599
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 6 cut(s) 641, 872, 1136, 1925, 1928, 1931
BspANI GGCC 6 cut(s) 623, 911, 1079, 1196, 1287, 1507
BspCNI CTCAG 6 cut(s) 166, 237, 1419, 1490, 1765, 1890
BspEI TCCGGA 1 cut(s) 599
BspFNI CGCG 2 cut(s) 328, 1064
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 4 cut(s) 782, 864, 955, 1833
BspMI ACCTGC 2 cut(s) 693, 1385
BspOI GCTAGC 1 cut(s) 1148
BspPI GGATC 3 cut(s) 65, 1622, 1813
BspQI GCTCTTC 2 cut(s) 1073, 1715
BspT104I TTCGAA 1 cut(s) 576
BspT107I GGYRCC 2 cut(s) 780, 953
BsrFI RCCGGY 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 4 cut(s) 22, 955, 1306, 1501
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 3 cut(s) 912, 983, 1197
BssMI GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
BssNAI GTATAC 1 cut(s) 1983
BssT1I CCWWGG 2 cut(s) 912, 1197
Bst1107I GTATAC 1 cut(s) 1983
Bst2UI CCWGG 2 cut(s) 747, 963
Bst4CI ACNGT 5 cut(s) 232, 1228, 1371, 1411, 1486
Bst6I CTCTTC 6 cut(s) 65, 924, 1073, 1536, 1715, 2009
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 828
BstBAI YACGTR 1 cut(s) 275
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 4 cut(s) 417, 701, 1146, 1233
BstDEI CTNAG 7 cut(s) 153, 245, 444, 1427, 1477, 1752, 1898
BstDSI CCRYGG 1 cut(s) 1197
BstEII GGTNACC 1 cut(s) 1912
BstENI CCTNNNNNAGG 1 cut(s) 1826
BstF5I GGATG 5 cut(s) 18, 41, 817, 831, 1942
BstFNI CGCG 2 cut(s) 328, 1064
BstHHI GCGC 3 cut(s) 330, 1066, 1977
BstKTI GATC 7 cut(s) 60, 107, 138, 558, 1255, 1617, 1821
BstMAI GTCTC 2 cut(s) 792, 1718
BstMBI GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 7 cut(s) 647, 685, 932, 941, 1691, 1786, 1930
BstNI CCWGG 2 cut(s) 747, 963
BstNSI RCATGY 3 cut(s) 121, 148, 419
BstPI GGTNACC 1 cut(s) 1912
BstSCI CCNGG 3 cut(s) 745, 961, 982
BstSLI GKGCMC 2 cut(s) 1369, 1782
BstUI CGCG 2 cut(s) 328, 1064
BstV1I GCAGC 7 cut(s) 708, 744, 931, 953, 1281, 1681, 1981
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 4 cut(s) 135, 1252, 1614, 1818
BstYI RGATCY 4 cut(s) 135, 1252, 1614, 1818
BstZ17I GTATAC 1 cut(s) 1983
BsuRI GGCC 6 cut(s) 623, 911, 1079, 1196, 1287, 1507
BtgI CCRYGG 1 cut(s) 1197
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 5 cut(s) 18, 41, 817, 831, 1942
BtsIMutI CAGTG 4 cut(s) 1249, 1398, 1482, 1494
BveI ACCTGC 2 cut(s) 693, 1385
Cac8I GCNNGC 4 cut(s) 417, 701, 1146, 1233
CaiI CAGNNNCTG 1 cut(s) 1484
CciI TCATGA 1 cut(s) 1318
CfoI GCGC 3 cut(s) 330, 1066, 1977
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 294, 749
CspAI ACCGGT 1 cut(s) 348
CspCI CAANNNNNGTGG 4 cut(s) 840, 875, 946, 981
DdeI CTNAG 7 cut(s) 153, 245, 444, 1427, 1477, 1752, 1898
DpnI GATC 7 cut(s) 59, 106, 137, 557, 1254, 1616, 1820
DpnII GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
EaeI YGGCCR 2 cut(s) 1285, 1505
Eam1104I CTCTTC 6 cut(s) 65, 924, 1073, 1536, 1715, 2009
EarI CTCTTC 6 cut(s) 65, 924, 1073, 1536, 1715, 2009
EciI GGCGGA 1 cut(s) 656
Eco130I CCWWGG 2 cut(s) 912, 1197
Eco147I AGGCCT 2 cut(s) 623, 1079
Eco47I GGWCC 2 cut(s) 294, 749
Eco57I CTGAAG 4 cut(s) 89, 948, 1370, 1560
Eco88I CYCGRG 3 cut(s) 691, 1129, 1416
Eco91I GGTNACC 1 cut(s) 1912
EcoNI CCTNNNNNAGG 1 cut(s) 1826
EcoO65I GGTNACC 1 cut(s) 1912
EcoRI GAATTC 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 745, 961
EcoT14I CCWWGG 2 cut(s) 912, 1197
EcoT22I ATGCAT 2 cut(s) 20, 123
ErhI CCWWGG 2 cut(s) 912, 1197
Esp3I CGTCTC 1 cut(s) 792
FalI AAGNNNNNCTT 2 cut(s) 1194, 1226
FaqI GGGAC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 3 cut(s) 227, 1965, 1982
FokI GGATG 5 cut(s) 5, 48, 824, 838, 1949
FspBI CTAG 5 cut(s) 363, 822, 989, 1145, 1340
GlaI GCGC 3 cut(s) 329, 1065, 1976
GsaI CCCAGC 1 cut(s) 1125
HaeIII GGCC 6 cut(s) 623, 911, 1079, 1196, 1287, 1507
HapII CCGG 3 cut(s) 349, 600, 983
HhaI GCGC 3 cut(s) 330, 1066, 1977
Hin6I GCGC 3 cut(s) 328, 1064, 1975
HinP1I GCGC 3 cut(s) 328, 1064, 1975
HincII GTYRAC 2 cut(s) 228, 1966
HindII GTYRAC 2 cut(s) 228, 1966
HindIII AAGCTT 1 cut(s) 1460
HinfI GANTC 8 cut(s) 89, 224, 377, 596, 773, 886, 1750, 1759
HpaII CCGG 3 cut(s) 349, 600, 983
HphI GGTGA 8 cut(s) 52, 646, 989, 1316, 1366, 1523, 1879, 1906
Hpy188I TCNGA 9 cut(s) 94, 104, 109, 156, 246, 456, 1525, 1723, 1741
Hpy99I CGWCG 3 cut(s) 215, 232, 940
HpyCH4III ACNGT 5 cut(s) 232, 1228, 1371, 1411, 1486
HpyCH4IV ACGT 2 cut(s) 274, 478
HpyF10VI GCNNNNNNNGC 7 cut(s) 647, 685, 932, 941, 1691, 1786, 1930
HpyF3I CTNAG 7 cut(s) 153, 245, 444, 1427, 1477, 1752, 1898
HpySE526I ACGT 2 cut(s) 274, 478
HspAI GCGC 3 cut(s) 328, 1064, 1975
Kpn2I TCCGGA 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
LguI GCTCTTC 2 cut(s) 1073, 1715
LmnI GCTCC 6 cut(s) 4, 94, 412, 487, 1707, 1831
Lsp1109I GCAGC 7 cut(s) 708, 744, 931, 953, 1281, 1681, 1981
LweI GCATC 3 cut(s) 27, 130, 1981
MaeI CTAG 5 cut(s) 363, 822, 989, 1145, 1340
MaeII ACGT 2 cut(s) 274, 478
MalI GATC 7 cut(s) 59, 106, 137, 557, 1254, 1616, 1820
MboI GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
MflI RGATCY 4 cut(s) 135, 1252, 1614, 1818
MhlI GDGCHC 2 cut(s) 1369, 1782
MlsI TGGCCA 1 cut(s) 1507
MluCI AATT 7 cut(s) 75, 537, 1139, 1265, 1358, 1501, 1630
MluNI TGGCCA 1 cut(s) 1507
MlyI GAGTC 3 cut(s) 233, 782, 1744
MmeI TCCRAC 4 cut(s) 693, 840, 1430, 1449
Mox20I TGGCCA 1 cut(s) 1507
Mph1103I ATGCAT 2 cut(s) 20, 123
MroI TCCGGA 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 1606
MscI TGGCCA 1 cut(s) 1507
MseI TTAA 4 cut(s) 408, 882, 999, 1044
MslI CAYNNNNRTG 3 cut(s) 1320, 1323, 1919
Msp20I TGGCCA 1 cut(s) 1507
MspA1I CMGCKG 2 cut(s) 757, 944
MspI CCGG 3 cut(s) 349, 600, 983
MspR9I CCNGG 3 cut(s) 747, 963, 984
Mva1269I GAATGC 2 cut(s) 1183, 1338
MvaI CCWGG 2 cut(s) 747, 963
MvnI CGCG 2 cut(s) 328, 1064
MwoI GCNNNNNNNGC 7 cut(s) 647, 685, 932, 941, 1691, 1786, 1930
NciI CCSGG 1 cut(s) 984
NcoI CCATGG 1 cut(s) 1197
NdeII GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
NheI GCTAGC 1 cut(s) 1144
NlaIV GGNNCC 4 cut(s) 782, 864, 955, 1833
NmeAIII GCCGAG 1 cut(s) 809
NmuCI GTSAC 7 cut(s) 1244, 1396, 1480, 1747, 1859, 1912, 1951
NsiI ATGCAT 2 cut(s) 20, 123
NspI RCATGY 3 cut(s) 121, 148, 419
NspV TTCGAA 1 cut(s) 576
OliI CACNNNNGTG 1 cut(s) 1919
PaeI GCATGC 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 1129
PagI TCATGA 1 cut(s) 1318
PaqCI CACCTGC 1 cut(s) 1385
PceI AGGCCT 2 cut(s) 623, 1079
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 2 cut(s) 1073, 1715
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 2 cut(s) 1183, 1338
PdmI GAANNNNTTC 1 cut(s) 1606
PfeI GAWTC 5 cut(s) 89, 377, 596, 886, 1759
PflMI CCANNNNNTGG 2 cut(s) 968, 1444
PinAI ACCGGT 1 cut(s) 348
PleI GAGTC 3 cut(s) 232, 781, 1744
PpsI GAGTC 3 cut(s) 232, 781, 1744
Ppu21I YACGTR 1 cut(s) 275
PscI ACATGT 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 745, 961
PspEI GGTNACC 1 cut(s) 1912
PspFI CCCAGC 1 cut(s) 1121
PspGI CCWGG 2 cut(s) 745, 961
PspN4I GGNNCC 4 cut(s) 782, 864, 955, 1833
PspPI GGNCC 2 cut(s) 294, 749
PspXI VCTCGAGB 1 cut(s) 1129
PstNI CAGNNNCTG 1 cut(s) 1484
PsuI RGATCY 4 cut(s) 135, 1252, 1614, 1818
PvuII CAGCTG 2 cut(s) 757, 944
RseI CAYNNNNRTG 3 cut(s) 1320, 1323, 1919
SalI GTCGAC 2 cut(s) 226, 1964
SapI GCTCTTC 2 cut(s) 1073, 1715
SaqAI TTAA 4 cut(s) 408, 882, 999, 1044
Sau3AI GATC 7 cut(s) 57, 104, 135, 555, 1252, 1614, 1818
Sau96I GGNCC 2 cut(s) 294, 749
ScaI AGTACT 2 cut(s) 608, 1008
SchI GAGTC 3 cut(s) 233, 782, 1744
ScrFI CCNGG 3 cut(s) 747, 963, 984
SduI GDGCHC 2 cut(s) 1369, 1782
SfaNI GCATC 3 cut(s) 27, 130, 1981
Sfr274I CTCGAG 1 cut(s) 1129
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 2 cut(s) 294, 749
SlaI CTCGAG 1 cut(s) 1129
SmiMI CAYNNNNRTG 3 cut(s) 1320, 1323, 1919
SmlI CTYRAG 4 cut(s) 383, 1010, 1129, 1169
SmoI CTYRAG 4 cut(s) 383, 1010, 1129, 1169
SphI GCATGC 1 cut(s) 419
Sse9I AATT 7 cut(s) 75, 537, 1139, 1265, 1358, 1501, 1630
SseBI AGGCCT 2 cut(s) 623, 1079
SsiI CCGC 6 cut(s) 641, 872, 1136, 1925, 1928, 1931
SspMI CTAG 5 cut(s) 363, 822, 989, 1145, 1340
StuI AGGCCT 2 cut(s) 623, 1079
StyD4I CCNGG 3 cut(s) 745, 961, 982
StyI CCWWGG 2 cut(s) 912, 1197
TaaI ACNGT 5 cut(s) 232, 1228, 1371, 1411, 1486
TaiI ACGT 2 cut(s) 277, 481
TasI AATT 7 cut(s) 75, 537, 1139, 1265, 1358, 1501, 1630
TatI WGTACW 8 cut(s) 149, 216, 606, 828, 1006, 1026, 1039, 1812
TauI GCSGC 4 cut(s) 875, 1927, 1930, 1933
TfiI GAWTC 5 cut(s) 89, 377, 596, 886, 1759
Tru1I TTAA 4 cut(s) 408, 882, 999, 1044
Tru9I TTAA 4 cut(s) 408, 882, 999, 1044
TscAI CASTG 4 cut(s) 1249, 1405, 1489, 1501
TseFI GTSAC 7 cut(s) 1244, 1396, 1480, 1747, 1859, 1912, 1951
TseI GCWGC 7 cut(s) 696, 757, 941, 944, 1294, 1694, 1994
Tsp45I GTSAC 7 cut(s) 1244, 1396, 1480, 1747, 1859, 1912, 1951
TspRI CASTG 4 cut(s) 1249, 1405, 1489, 1501
Van91I CCANNNNNTGG 2 cut(s) 968, 1444
VneI GTGCAC 2 cut(s) 1365, 1778
VpaK11BI GGWCC 2 cut(s) 294, 749
XagI CCTNNNNNAGG 1 cut(s) 1826
XapI RAATTY 2 cut(s) 75, 1265
XceI RCATGY 3 cut(s) 121, 148, 419
XhoI CTCGAG 1 cut(s) 1129
XmiI GTMKAC 3 cut(s) 227, 1965, 1982
XmnI GAANNNNTTC 1 cut(s) 1606
XspI CTAG 5 cut(s) 363, 822, 989, 1145, 1340
ZrmI AGTACT 2 cut(s) 608, 1008
Zsp2I ATGCAT 2 cut(s) 20, 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.