Rroxscaffold_1G00041650

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000162
Physical Location & Seq
Reverse (-)
59557162 .. 59559401
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00041650.1

Sequence Viewer

Length: 2214 bp
ATGGAGAGACAAATTATCTTGCTTGATGAAGGATGGGGCCATATGCAGAATGGGATCACCAAGCTGAAGAGAATTCTCGAAGGACTACCGGAGCCTCAGTTCAGCTCACAGGAATACATAGATCTTTACACAGTGATTTACAACATGTGTGTTCAGAAGCCCCCTCACGACTACTCTGTACAGCTCTATAATAATTACGGCGAGGTCTTCGTCGAGTACATCGAGTCGACGGTGTTGCCTTCCCTGAGAGAGAAGCACGACGAGTTCATGATGCGTGAGCTCGTCCGAAGATGGTCCAATCACAAACTCATGGTCCGGTGGCTCACTCGCTTCTTTCAGTATCTCGACCGCTACTACGTCTGCCGAAACTCCAAGGATCCGCTCAAGGATGCTGGACTTAAGCGATTTAAGGATCACGTTTACAACGAGATGAATGCAAAGGCTAGGGACTCTCTGATAGCTCTTATTTTGAGGGAACGTGAAGGGGAGCAGATTGACAGAGCGCTGATGAAGAACGTGGTTGATATATTCGTGGAGGTTGGAATGGGAGAGATGGTGAATTACGAGGAGGAGTTCGAAAAACACATGGTTGATGATACTGGGGACTACTACTCTCGAAAGGCCTCGATTTGGATTGTGGAGGATTCTTGTCCCGAGTACATGATGAAGGCGGAGGAGGCCTTGAGGAAGGAGAAGGAGAGAGTTTCCCATTACCTACATTCGGGCAGCGAGCAAAAGCTGTTGGAGAGGGTGCAGCAAGAGTTGTTGGTGGTTCAAGGTCCACAGCTGCTTGATAAAGAGGGGTCTGGATGCCGGACCTTGCTTCGAGACGATAGGGTGGATGACTTGTCTAGGATGTTCAGGCTTTACCATAAAATACCCCAAGGGTTGGATCCTGTTGCTGCTATGTTTAAGAAGCATGTTACTAATGAAGGCACAGCCTTGGTCCAACAGGCCGAAGAGGCAACAGCAGCTGCAAGTGGCAACAAGCAAGGTGGTGGTGCTGGTGAGCCGGGACTAGTATTTATCAGCAAAATACTTGAGCTACATGATAAGTACATGGGATATGTCCTTAGCTGCTTCCAAAACCACTCGCTCTTCCACAAGGCCTTGAAAGAGGCTTTTGAGGTATTCTGTAATAAATCTGTTGCTGGGTGCTCGAGTGCAGAATTGCTTGCTACATTCTGTGATAGTATCCTCAAGCAGATGGGTGAGAGTGACGAGGCCAAAGAAGAAACTCTTGACAAGATTGTTACGGTTCTTGCATATTTCAGTGACAAGGATCTTTTCGCCGAATTCTGCAGGAAGAAACTTGCCCGTAGGCTGCTCATGGTTCATGATGATAAGAAGAACCGTGCTGACCAGCTTGACAATGAGAGAATGCTTCTAACAAAGCTGAAACAGCAATGTGGAGGACAGTTCACCTCTAAGATGGAGGGAATGGTCACAGATATTACTATTTCTCGGGAAAGCGAGAAGAGTTTCAAAGACTATCTTCGAAAAGATGGTACTCCAAAGCTTGAGTTGGATTTCTCAGTCACTGTTCTAACAACTGGTAACTGGCCAAGCTATAAAACATCTGATCTTCACCTTCCTGAAGAGATGGTCAAGTGCATTGAAGTGTATAAAGAATATTATGACAGGGAAAAGAAACACAGGAAGCTTTCATGGATTTATTCATTGGGTACTTGCCATGTTAGGGGCAACTTTGAGCCAAAACCCATTGAAATGCTTATGTCAACCTATCAGGCTGCTCTCCTGCTACTCTTCAACACCTCGGAGAAATTGAGCTTTTCAGAAATATTGACTCAATTAAATCTTACCAAAGATGATGTCCACAGAACGCTTCATTCATTTTCTTGTGCCAAGTACAAGATCCTTCTCAAAGAGCCTAACACAAAGACAATCTCCCCATTCACCGACAGATTGAGGAAAATCAGGATCCCTCTGCCACCGCTGCAGGATGAAAGGAAGAAGGTCACGGAAGATGTCGACAAAGAGCGCAAGTATACAATTGACGCTGCACTGGTGAGGATTATGAAGAGTCGAAAAATATTGGGTCATCAACAACTGGTCACTGAGTGTCTTGAACAGTTGCAGCGCACGTTCAAGCCAGACATCAAAACAATCAAGAGGCGCATTGAAGATCTCATCACCCGTGACTACCTTGAGAGGGACCCTGAGAATCCTAACACATTCAAGTATGTCGCATGA

Protein Analysis

737

Amino Acids

86.02

Weight (kDa)

6.87

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 25 - 490 4.8e-120 Cullin alpha solenoid domain
Cullin_AB PF26557 515 - 638 7.1e-38 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 667 - 729 2.3e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 3 cut(s) 227, 1992, 2009
AciI CCGC 4 cut(s) 349, 380, 671, 1955
AcoI YGGCCR 1 cut(s) 1562
AcsI RAATTY 2 cut(s) 72, 1295
AcuI CTGAAG 2 cut(s) 86, 1617
AdeI CACNNNGTG 1 cut(s) 2082
AfaI GTAC 7 cut(s) 180, 218, 659, 1058, 1510, 1687, 1871
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1699, 2173
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 1018
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
Alw21I GWGCWC 2 cut(s) 282, 1160
Alw26I GTCTC 1 cut(s) 822
AlwNI CAGNNNCTG 2 cut(s) 974, 1541
Ama87I CYCGRG 3 cut(s) 653, 1159, 1464
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 7 cut(s) 37, 621, 678, 954, 1107, 1224, 1562
ApoI RAATTY 2 cut(s) 72, 1295
ArsI GACNNNNNNTTYG 4 cut(s) 297, 329, 1818, 1850
Asp700I GAANNNNTTC 1 cut(s) 1481
AspLEI GCGC 4 cut(s) 505, 2004, 2103, 2139
AspS9I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2176
AsuC2I CCSGG 1 cut(s) 1014
AsuHPI GGTGA 9 cut(s) 49, 568, 1019, 1223, 1414, 1580, 1909, 2041, 2146
AsuII TTCGAA 2 cut(s) 576, 1498
AvaI CYCGRG 3 cut(s) 653, 1159, 1464
AvaII GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2176
BalI TGGCCA 1 cut(s) 1564
BamHI GGATCC 3 cut(s) 376, 892, 1941
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 2 cut(s) 282, 1160
BccI CCATC 7 cut(s) 27, 285, 547, 1201, 1426, 1499, 1597
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 4 cut(s) 217, 251, 416, 450
BciVI GTATCC 1 cut(s) 1205
BcnI CCSGG 1 cut(s) 1014
BcoDI GTCTC 1 cut(s) 822
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 3 cut(s) 444, 852, 1019
BfmI CTRYAG 2 cut(s) 1300, 1958
BfoI RGCGCY 1 cut(s) 506
BfrI CTTAAG 1 cut(s) 398
BfuI GTATCC 1 cut(s) 1205
BglI GCCNNNNNGGC 1 cut(s) 962
BglII AGATCT 2 cut(s) 121, 2146
Bme1390I CCNGG 1 cut(s) 1014
Bme18I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2176
BmeT110I CYCGRG 3 cut(s) 653, 1159, 1464
BmgT120I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2176
BmiI GGNNCC 7 cut(s) 38, 93, 378, 894, 1943, 2177, 2178
BmrFI CCNGG 1 cut(s) 1014
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 3 cut(s) 261, 379, 800
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 1073
Bpu14I TTCGAA 2 cut(s) 576, 1498
BpuEI CTTGAG 6 cut(s) 368, 703, 1061, 1184, 1541, 2189
BpuMI CCSGG 1 cut(s) 1014
BsaJI CCNNGG 4 cut(s) 372, 883, 942, 1776
BsaWI WCCGGW 2 cut(s) 88, 315
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 540, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1699, 2173
Bse1I ACTGG 5 cut(s) 604, 1558, 1565, 2031, 2076
Bse3DI GCAATG 1 cut(s) 1412
BseDI CCNNGG 4 cut(s) 372, 883, 942, 1776
BseGI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1969
BseLI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1699, 2173
BseMI GCAATG 1 cut(s) 1412
BseMII CTCAG 5 cut(s) 110, 236, 1548, 2070, 2172
BseNI ACTGG 5 cut(s) 604, 1558, 1565, 2031, 2076
BseRI GAGGAG 3 cut(s) 581, 584, 689
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 3 cut(s) 773, 1185, 2007
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 2 cut(s) 282, 1160
BsiHKCI CYCGRG 3 cut(s) 653, 1159, 1464
BsiSI CCGG 4 cut(s) 89, 316, 814, 1013
BslFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2189
BslI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1699, 2173
BsmAI GTCTC 1 cut(s) 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2189
BsmI GAATGC 2 cut(s) 439, 1386
BsnI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BsoBI CYCGRG 3 cut(s) 653, 1159, 1464
Bsp119I TTCGAA 2 cut(s) 576, 1498
Bsp1286I GDGCHC 2 cut(s) 282, 1160
Bsp1407I TGTACA 1 cut(s) 178
BspACI CCGC 4 cut(s) 349, 380, 671, 1955
BspANI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BspCNI CTCAG 5 cut(s) 109, 237, 1547, 2071, 2173
BspHI TCATGA 2 cut(s) 267, 1336
BspLI GGNNCC 7 cut(s) 38, 93, 378, 894, 1943, 2177, 2178
BspMAI CTGCAG 2 cut(s) 1304, 1962
BspQI GCTCTTC 1 cut(s) 1103
BspT104I TTCGAA 2 cut(s) 576, 1498
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 1412
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 5 cut(s) 604, 1558, 1565, 2031, 2076
BssECI CCNNGG 4 cut(s) 372, 883, 942, 1776
BssNAI GTATAC 1 cut(s) 2010
BssT1I CCWWGG 3 cut(s) 372, 883, 942
Bst1107I GTATAC 1 cut(s) 2010
Bst4CI ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2094
Bst6I CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 2036
BstAFI CTTAAG 1 cut(s) 398
BstAUI TGTACA 1 cut(s) 178
BstBI TTCGAA 2 cut(s) 576, 1498
BstC8I GCNNGC 2 cut(s) 731, 1176
BstDEI CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2079, 2181
BstF5I GGATG 6 cut(s) 38, 394, 815, 847, 861, 1969
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 4 cut(s) 505, 2004, 2103, 2139
BstMAI GTCTC 1 cut(s) 822
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1957
BstNSI RCATGY 2 cut(s) 148, 923
BstSCI CCNGG 1 cut(s) 1012
BstSFI CTRYAG 2 cut(s) 1300, 1958
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 7 cut(s) 121, 376, 892, 1282, 1875, 1941, 2146
BstYI RGATCY 7 cut(s) 121, 376, 892, 1282, 1875, 1941, 2146
BstZ17I GTATAC 1 cut(s) 2010
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BtsCI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1969
BtsIMutI CAGTG 5 cut(s) 138, 1279, 1539, 2024, 2076
Cac8I GCNNGC 2 cut(s) 731, 1176
CaiI CAGNNNCTG 2 cut(s) 974, 1541
CciI TCATGA 2 cut(s) 267, 1336
CfoI GCGC 4 cut(s) 505, 2004, 2103, 2139
Cfr13I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2176
CseI GACGC 1 cut(s) 2027
Csp6I GTAC 7 cut(s) 179, 217, 658, 1057, 1509, 1686, 1870
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 7 cut(s) 179, 217, 658, 1057, 1509, 1686, 1870
DdeI CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2079, 2181
DraIII CACNNNGTG 1 cut(s) 2082
EaeI YGGCCR 1 cut(s) 1562
Eam1104I CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 2036
EarI CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 2036
EciI GGCGGA 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 372, 883, 942
Eco147I AGGCCT 3 cut(s) 623, 680, 1109
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2176
Eco47III AGCGCT 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 86, 1617
Eco88I CYCGRG 3 cut(s) 653, 1159, 1464
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 2176
EcoRI GAATTC 2 cut(s) 72, 1295
EcoT14I CCWWGG 3 cut(s) 372, 883, 942
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 3 cut(s) 372, 883, 942
Esp3I CGTCTC 1 cut(s) 822
FalI AAGNNNNNCTT 4 cut(s) 1224, 1256, 1479, 1511
FaqI GGGAC 5 cut(s) 461, 617, 636, 1029, 2189
FauNDI CATATG 1 cut(s) 42
FblI GTMKAC 3 cut(s) 227, 1992, 2009
FokI GGATG 6 cut(s) 45, 401, 822, 854, 868, 1976
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 3 cut(s) 444, 852, 1019
GlaI GCGC 4 cut(s) 504, 2003, 2102, 2138
GsaI CCCAGC 1 cut(s) 1155
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
HapII CCGG 4 cut(s) 89, 316, 814, 1013
HgaI GACGC 1 cut(s) 2027
HhaI GCGC 4 cut(s) 505, 2004, 2103, 2139
Hin6I GCGC 4 cut(s) 503, 2002, 2101, 2137
HinP1I GCGC 4 cut(s) 503, 2002, 2101, 2137
HincII GTYRAC 3 cut(s) 228, 1740, 1993
HindII GTYRAC 3 cut(s) 228, 1740, 1993
HindIII AAGCTT 2 cut(s) 1517, 1661
HinfI GANTC 6 cut(s) 224, 449, 644, 1807, 2044, 2185
HpaII CCGG 4 cut(s) 89, 316, 814, 1013
HphI GGTGA 9 cut(s) 49, 568, 1019, 1223, 1414, 1580, 1909, 2041, 2146
Hpy166II GTNNAC 8 cut(s) 228, 421, 782, 1422, 1740, 1837, 1993, 2010
Hpy188I TCNGA 6 cut(s) 156, 287, 456, 1582, 1780, 1798
Hpy8I GTNNAC 8 cut(s) 228, 421, 782, 1422, 1740, 1837, 1993, 2010
Hpy99I CGWCG 3 cut(s) 215, 232, 263
HpyCH4III ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2094
HpyCH4IV ACGT 5 cut(s) 357, 417, 478, 516, 2105
HpyF10VI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1957
HpyF3I CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2079, 2181
HpySE526I ACGT 5 cut(s) 357, 417, 478, 516, 2105
HspAI GCGC 4 cut(s) 503, 2002, 2101, 2137
KflI GGGWCCC 1 cut(s) 2176
LguI GCTCTTC 1 cut(s) 1103
LmnI GCTCC 2 cut(s) 91, 487
LweI GCATC 3 cut(s) 261, 379, 800
MaeI CTAG 3 cut(s) 444, 852, 1019
MaeII ACGT 5 cut(s) 357, 417, 478, 516, 2105
MbiI CCGCTC 1 cut(s) 382
MfeI CAATTG 1 cut(s) 2013
MflI RGATCY 7 cut(s) 121, 376, 892, 1282, 1875, 1941, 2146
MhlI GDGCHC 2 cut(s) 282, 1160
MlsI TGGCCA 1 cut(s) 1564
MluCI AATT 9 cut(s) 12, 72, 193, 559, 1169, 1295, 1784, 1811, 2013
MluNI TGGCCA 1 cut(s) 1564
MlyI GAGTC 4 cut(s) 233, 443, 1801, 2053
MmeI TCCRAC 5 cut(s) 520, 723, 870, 973, 1506
Mox20I TGGCCA 1 cut(s) 1564
MroXI GAANNNNTTC 1 cut(s) 1481
MscI TGGCCA 1 cut(s) 1564
MseI TTAA 4 cut(s) 399, 408, 912, 1814
MslI CAYNNNNRTG 2 cut(s) 1619, 1727
Msp20I TGGCCA 1 cut(s) 1564
MspA1I CMGCKG 3 cut(s) 787, 974, 1957
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 4 cut(s) 89, 316, 814, 1013
MspR9I CCNGG 1 cut(s) 1014
MunI CAATTG 1 cut(s) 2013
Mva1269I GAATGC 2 cut(s) 439, 1386
MwoI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1957
NciI CCSGG 1 cut(s) 1014
NdeI CATATG 1 cut(s) 42
NlaIV GGNNCC 7 cut(s) 38, 93, 378, 894, 1943, 2177, 2178
NmuCI GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1978, 2074, 2159
NspI RCATGY 2 cut(s) 148, 923
NspV TTCGAA 2 cut(s) 576, 1498
PaeR7I CTCGAG 1 cut(s) 1159
PagI TCATGA 2 cut(s) 267, 1336
PceI AGGCCT 3 cut(s) 623, 680, 1109
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 1103
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 423
PctI GAATGC 2 cut(s) 439, 1386
PdmI GAANNNNTTC 1 cut(s) 1481
PfeI GAWTC 2 cut(s) 644, 2185
PflMI CCANNNNNTGG 1 cut(s) 889
PleI GAGTC 4 cut(s) 232, 443, 1801, 2052
PpsI GAGTC 4 cut(s) 232, 443, 1801, 2052
PpuMI RGGWCCY 1 cut(s) 2176
PscI ACATGT 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 2176
PspFI CCCAGC 1 cut(s) 1151
PspN4I GGNNCC 7 cut(s) 38, 93, 378, 894, 1943, 2177, 2178
PspPI GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2176
PspPPI RGGWCCY 1 cut(s) 2176
PspXI VCTCGAGB 1 cut(s) 1159
PstI CTGCAG 2 cut(s) 1304, 1962
PstNI CAGNNNCTG 2 cut(s) 974, 1541
PsuI RGATCY 7 cut(s) 121, 376, 892, 1282, 1875, 1941, 2146
PvuII CAGCTG 2 cut(s) 787, 974
RsaI GTAC 7 cut(s) 180, 218, 659, 1058, 1510, 1687, 1871
RsaNI GTAC 7 cut(s) 179, 217, 658, 1057, 1509, 1686, 1870
RseI CAYNNNNRTG 2 cut(s) 1619, 1727
SacI GAGCTC 1 cut(s) 282
SalI GTCGAC 2 cut(s) 226, 1991
SapI GCTCTTC 1 cut(s) 1103
SaqAI TTAA 4 cut(s) 399, 408, 912, 1814
Sau96I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2176
SchI GAGTC 4 cut(s) 233, 443, 1801, 2053
ScrFI CCNGG 1 cut(s) 1014
SduI GDGCHC 2 cut(s) 282, 1160
SfaNI GCATC 3 cut(s) 261, 379, 800
SfcI CTRYAG 2 cut(s) 1300, 1958
Sfr274I CTCGAG 1 cut(s) 1159
SfuI TTCGAA 2 cut(s) 576, 1498
SinI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2176
SlaI CTCGAG 1 cut(s) 1159
SmiMI CAYNNNNRTG 2 cut(s) 1619, 1727
SmlI CTYRAG 8 cut(s) 383, 398, 682, 1040, 1159, 1199, 1520, 2168
SmoI CTYRAG 8 cut(s) 383, 398, 682, 1040, 1159, 1199, 1520, 2168
SpeI ACTAGT 1 cut(s) 1018
Sse9I AATT 9 cut(s) 12, 72, 193, 559, 1169, 1295, 1784, 1811, 2013
SseBI AGGCCT 3 cut(s) 623, 680, 1109
SsiI CCGC 4 cut(s) 349, 380, 671, 1955
SspI AATATT 3 cut(s) 1634, 1803, 2055
SspMI CTAG 3 cut(s) 444, 852, 1019
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 3 cut(s) 623, 680, 1109
StyD4I CCNGG 1 cut(s) 1012
StyI CCWWGG 3 cut(s) 372, 883, 942
TaaI ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2094
TaiI ACGT 5 cut(s) 360, 420, 481, 519, 2108
TasI AATT 9 cut(s) 12, 72, 193, 559, 1169, 1295, 1784, 1811, 2013
TatI WGTACW 5 cut(s) 178, 216, 657, 1056, 1869
TfiI GAWTC 2 cut(s) 644, 2185
Tru1I TTAA 4 cut(s) 399, 408, 912, 1814
Tru9I TTAA 4 cut(s) 399, 408, 912, 1814
TscAI CASTG 5 cut(s) 138, 1279, 1546, 2031, 2083
TseFI GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1978, 2074, 2159
Tsp45I GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1978, 2074, 2159
TspGWI ACGGA 1 cut(s) 1997
TspRI CASTG 5 cut(s) 138, 1279, 1546, 2031, 2083
Van91I CCANNNNNTGG 1 cut(s) 889
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2176
XapI RAATTY 2 cut(s) 72, 1295
XceI RCATGY 2 cut(s) 148, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 1159
XmiI GTMKAC 3 cut(s) 227, 1992, 2009
XmnI GAANNNNTTC 1 cut(s) 1481
XspI CTAG 3 cut(s) 444, 852, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.