Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1464 |
| AccB1I | GGYRCC | 2 cut(s) | 1464, 1591 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 375 |
| AccI | GTMKAC | 2 cut(s) | 98, 1446 |
| AccII | CGCG | 1 cut(s) | 2060 |
| AciI | CCGC | 4 cut(s) | 713, 1589, 1625, 2058 |
| AclWI | GGATC | 7 cut(s) | 155, 503, 516, 1481, 1859, 1877, 1890 |
| AcoI | YGGCCR | 3 cut(s) | 473, 685, 714 |
| AcsI | RAATTY | 4 cut(s) | 192, 534, 1398, 2187 |
| AcuI | CTGAAG | 1 cut(s) | 741 |
| AfaI | GTAC | 4 cut(s) | 1278, 1466, 1722, 2108 |
| AfeI | AGCGCT | 1 cut(s) | 615 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 375, 677, 981, 1379, 1380 |
| AgsI | TTSAA | 7 cut(s) | 16, 86, 493, 1151, 1674, 2173, 2318 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 664 |
| AjnI | CCWGG | 3 cut(s) | 319, 1237, 1938 |
| Alw21I | GWGCWC | 2 cut(s) | 129, 595 |
| Alw26I | GTCTC | 3 cut(s) | 1250, 1519, 1740 |
| Alw44I | GTGCAC | 1 cut(s) | 125 |
| AlwI | GGATC | 7 cut(s) | 155, 503, 516, 1481, 1859, 1877, 1890 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 482 |
| Ama87I | CYCGRG | 2 cut(s) | 1385, 1886 |
| Aor51HI | AGCGCT | 1 cut(s) | 615 |
| ApaLI | GTGCAC | 1 cut(s) | 125 |
| ApeKI | GCWGC | 6 cut(s) | 224, 275, 1067, 1097, 1613, 1742 |
| ApoI | RAATTY | 4 cut(s) | 192, 534, 1398, 2187 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 660 |
| Asp718I | GGTACC | 1 cut(s) | 1464 |
| AspLEI | GCGC | 2 cut(s) | 616, 781 |
| AspS9I | GGNCC | 3 cut(s) | 1016, 1547, 1814 |
| AsuC2I | CCSGG | 3 cut(s) | 1596, 1887, 1888 |
| AsuHPI | GGTGA | 6 cut(s) | 560, 660, 1151, 1486, 2077, 2282 |
| AvaI | CYCGRG | 2 cut(s) | 1385, 1886 |
| AvaII | GGWCC | 1 cut(s) | 1547 |
| AxyI | CCTNAGG | 1 cut(s) | 1264 |
| BaeGI | GKGCMC | 1 cut(s) | 129 |
| BalI | TGGCCA | 2 cut(s) | 475, 687 |
| BamHI | GGATCC | 2 cut(s) | 508, 1882 |
| BanI | GGYRCC | 2 cut(s) | 1464, 1591 |
| BanII | GRGCYC | 1 cut(s) | 1386 |
| BauI | CACGAG | 1 cut(s) | 2130 |
| BbsI | GAAGAC | 1 cut(s) | 663 |
| Bbv12I | GWGCWC | 2 cut(s) | 129, 595 |
| BbvI | GCAGC | 6 cut(s) | 211, 262, 1079, 1109, 1600, 1754 |
| BccI | CCATC | 7 cut(s) | 402, 526, 671, 869, 1007, 1373, 1784 |
| BceAI | ACGGC | 2 cut(s) | 2099, 2282 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2111, 2145 |
| BciT130I | CCWGG | 3 cut(s) | 321, 1239, 1940 |
| BcnI | CCSGG | 3 cut(s) | 1596, 1887, 1888 |
| BcoDI | GTCTC | 3 cut(s) | 1250, 1519, 1740 |
| BfaI | CTAG | 5 cut(s) | 752, 1542, 1551, 1760, 1965 |
| BfmI | CTRYAG | 7 cut(s) | 244, 708, 900, 1200, 1209, 1740, 1908 |
| BfoI | RGCGCY | 2 cut(s) | 617, 782 |
| BisI | GCNGC | 8 cut(s) | 225, 276, 714, 1068, 1098, 1590, 1614, 1743 |
| BlpI | GCTNAGC | 1 cut(s) | 1000 |
| BlsI | GCNGC | 8 cut(s) | 226, 277, 715, 1069, 1099, 1591, 1615, 1744 |
| BmcAI | AGTACT | 1 cut(s) | 1278 |
| Bme1390I | CCNGG | 6 cut(s) | 321, 1239, 1596, 1887, 1888, 1940 |
| Bme18I | GGWCC | 1 cut(s) | 1547 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 664 |
| BmeT110I | CYCGRG | 2 cut(s) | 1385, 1886 |
| BmgT120I | GGNCC | 3 cut(s) | 1016, 1547, 1814 |
| BmiI | GGNNCC | 6 cut(s) | 510, 1466, 1593, 1884, 2127, 2252 |
| BmrFI | CCNGG | 6 cut(s) | 321, 1239, 1596, 1887, 1888, 1940 |
| BmsI | GCATC | 8 cut(s) | 176, 818, 826, 1165, 1328, 1964, 1996, 2284 |
| BpiI | GAAGAC | 1 cut(s) | 663 |
| BpmI | CTGGAG | 2 cut(s) | 390, 835 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1000 |
| BpuEI | CTTGAG | 1 cut(s) | 279 |
| BpuMI | CCSGG | 3 cut(s) | 1596, 1887, 1888 |
| BsaAI | YACGTR | 1 cut(s) | 941 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2317 |
| BsaI | GGTCTC | 2 cut(s) | 1519, 1740 |
| BsaJI | CCNNGG | 9 cut(s) | 671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 375, 677, 981, 1379, 1380 |
| Bse118I | RCCGGY | 1 cut(s) | 716 |
| Bse1I | ACTGG | 1 cut(s) | 1786 |
| Bse21I | CCTNAGG | 1 cut(s) | 1264 |
| Bse3DI | GCAATG | 4 cut(s) | 913, 1651, 1884, 2016 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2317 |
| BseBI | CCWGG | 3 cut(s) | 321, 1239, 1940 |
| BseDI | CCNNGG | 9 cut(s) | 671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115 |
| BseGI | GGATG | 4 cut(s) | 625, 682, 833, 885 |
| BseJI | GATNNNNATC | 1 cut(s) | 2317 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 375, 677, 981, 1379, 1380 |
| BseMI | GCAATG | 4 cut(s) | 913, 1651, 1884, 2016 |
| BseMII | CTCAG | 6 cut(s) | 303, 639, 1014, 1278, 1495, 2234 |
| BseNI | ACTGG | 1 cut(s) | 1786 |
| BseRI | GAGGAG | 3 cut(s) | 786, 789, 2118 |
| BseSI | GKGCMC | 1 cut(s) | 129 |
| BseX3I | CGGCCG | 1 cut(s) | 714 |
| BseXI | GCAGC | 6 cut(s) | 211, 262, 1079, 1109, 1600, 1754 |
| BsgI | GTGCAG | 3 cut(s) | 57, 1029, 2141 |
| Bsh1236I | CGCG | 1 cut(s) | 2060 |
| Bsh1285I | CGRYCG | 2 cut(s) | 717, 1625 |
| BshNI | GGYRCC | 2 cut(s) | 1464, 1591 |
| BsiEI | CGRYCG | 2 cut(s) | 717, 1625 |
| BsiHKAI | GWGCWC | 2 cut(s) | 129, 595 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1385, 1886 |
| BsiSI | CCGG | 3 cut(s) | 717, 1596, 1887 |
| BslFI | GGGAC | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 375, 677, 981, 1379, 1380 |
| BsmAI | GTCTC | 3 cut(s) | 1250, 1519, 1740 |
| BsmFI | GGGAC | 1 cut(s) | 43 |
| BsmI | GAATGC | 2 cut(s) | 348, 400 |
| Bso31I | GGTCTC | 2 cut(s) | 1519, 1740 |
| BsoBI | CYCGRG | 2 cut(s) | 1385, 1886 |
| Bsp1286I | GDGCHC | 3 cut(s) | 129, 595, 1386 |
| Bsp1407I | TGTACA | 1 cut(s) | 1720 |
| Bsp143I | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1000 |
| Bsp19I | CCATGG | 3 cut(s) | 688, 1049, 1162 |
| BspACI | CCGC | 4 cut(s) | 713, 1589, 1625, 2058 |
| BspCNI | CTCAG | 6 cut(s) | 304, 640, 1013, 1277, 1494, 2233 |
| BspFNI | CGCG | 1 cut(s) | 2060 |
| BspHI | TCATGA | 1 cut(s) | 871 |
| BspLI | GGNNCC | 6 cut(s) | 510, 1466, 1593, 1884, 2127, 2252 |
| BspMAI | CTGCAG | 2 cut(s) | 1744, 1912 |
| BspPI | GGATC | 7 cut(s) | 155, 503, 516, 1481, 1859, 1877, 1890 |
| BspT107I | GGYRCC | 2 cut(s) | 1464, 1591 |
| BspTNI | GGTCTC | 2 cut(s) | 1519, 1740 |
| BsrDI | GCAATG | 4 cut(s) | 913, 1651, 1884, 2016 |
| BsrFI | RCCGGY | 1 cut(s) | 716 |
| BsrGI | TGTACA | 1 cut(s) | 1720 |
| BsrI | ACTGG | 1 cut(s) | 1786 |
| BssAI | RCCGGY | 1 cut(s) | 716 |
| BssECI | CCNNGG | 9 cut(s) | 671, 688, 1049, 1162, 1224, 1886, 1938, 1939, 2115 |
| BssMI | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| BssNAI | GTATAC | 1 cut(s) | 99 |
| BssSI | CACGAG | 1 cut(s) | 2130 |
| BssT1I | CCWWGG | 4 cut(s) | 688, 1049, 1162, 1224 |
| Bst1107I | GTATAC | 1 cut(s) | 99 |
| Bst2BI | CACGAG | 1 cut(s) | 2130 |
| Bst2UI | CCWGG | 3 cut(s) | 321, 1239, 1940 |
| Bst4CI | ACNGT | 9 cut(s) | 991, 1063, 1330, 1368, 1498, 2065, 2077, 2149, 2270 |
| BstAUI | TGTACA | 1 cut(s) | 1720 |
| BstBAI | YACGTR | 1 cut(s) | 941 |
| BstC8I | GCNNGC | 6 cut(s) | 542, 595, 718, 811, 1174, 2124 |
| BstDEI | CTNAG | 7 cut(s) | 312, 648, 793, 1000, 1264, 1481, 2220 |
| BstDSI | CCRYGG | 4 cut(s) | 688, 1049, 1162, 2115 |
| BstF5I | GGATG | 4 cut(s) | 625, 682, 833, 885 |
| BstFNI | CGCG | 1 cut(s) | 2060 |
| BstH2I | RGCGCY | 2 cut(s) | 617, 782 |
| BstHHI | GCGC | 2 cut(s) | 616, 781 |
| BstKTI | GATC | 6 cut(s) | 163, 511, 937, 1489, 1867, 1885 |
| BstMAI | GTCTC | 3 cut(s) | 1250, 1519, 1740 |
| BstMBI | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| BstMCI | CGRYCG | 2 cut(s) | 717, 1625 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 550, 710, 785, 794, 1094, 1259, 1821 |
| BstNI | CCWGG | 3 cut(s) | 321, 1239, 1940 |
| BstNSI | RCATGY | 1 cut(s) | 1683 |
| BstSCI | CCNGG | 6 cut(s) | 319, 1237, 1594, 1885, 1886, 1938 |
| BstSFI | CTRYAG | 7 cut(s) | 244, 708, 900, 1200, 1209, 1740, 1908 |
| BstSLI | GKGCMC | 1 cut(s) | 129 |
| BstUI | CGCG | 1 cut(s) | 2060 |
| BstV1I | GCAGC | 6 cut(s) | 211, 262, 1079, 1109, 1600, 1754 |
| BstV2I | GAAGAC | 1 cut(s) | 663 |
| BstX2I | RGATCY | 2 cut(s) | 508, 1882 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 525 |
| BstYI | RGATCY | 2 cut(s) | 508, 1882 |
| BstZ17I | GTATAC | 1 cut(s) | 99 |
| BstZI | CGGCCG | 1 cut(s) | 714 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1264 |
| BtgI | CCRYGG | 4 cut(s) | 688, 1049, 1162, 2115 |
| BtsCI | GGATG | 4 cut(s) | 625, 682, 833, 885 |
| BtsI | GCAGTG | 2 cut(s) | 1041, 1655 |
| BtsIMutI | CAGTG | 6 cut(s) | 831, 1041, 1059, 1489, 1655, 2082 |
| Cac8I | GCNNGC | 6 cut(s) | 542, 595, 718, 811, 1174, 2124 |
| CaiI | CAGNNNCTG | 1 cut(s) | 482 |
| CciI | TCATGA | 1 cut(s) | 871 |
| CfoI | GCGC | 2 cut(s) | 616, 781 |
| Cfr10I | RCCGGY | 1 cut(s) | 716 |
| Cfr13I | GGNCC | 3 cut(s) | 1016, 1547, 1814 |
| Cfr9I | CCCGGG | 1 cut(s) | 1886 |
| Csp6I | GTAC | 4 cut(s) | 1277, 1465, 1721, 2107 |
| CviQI | GTAC | 4 cut(s) | 1277, 1465, 1721, 2107 |
| DdeI | CTNAG | 7 cut(s) | 312, 648, 793, 1000, 1264, 1481, 2220 |
| DpnI | GATC | 6 cut(s) | 162, 510, 936, 1488, 1866, 1884 |
| DpnII | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| DraI | TTTAAA | 1 cut(s) | 1575 |
| DriI | GACNNNNNGTC | 1 cut(s) | 664 |
| EaeI | YGGCCR | 3 cut(s) | 473, 685, 714 |
| EagI | CGGCCG | 1 cut(s) | 714 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 664 |
| EclXI | CGGCCG | 1 cut(s) | 714 |
| Eco130I | CCWWGG | 4 cut(s) | 688, 1049, 1162, 1224 |
| Eco147I | AGGCCT | 1 cut(s) | 1176 |
| Eco24I | GRGCYC | 1 cut(s) | 1386 |
| Eco31I | GGTCTC | 2 cut(s) | 1519, 1740 |
| Eco47I | GGWCC | 1 cut(s) | 1547 |
| Eco47III | AGCGCT | 1 cut(s) | 615 |
| Eco52I | CGGCCG | 1 cut(s) | 714 |
| Eco57I | CTGAAG | 1 cut(s) | 741 |
| Eco81I | CCTNAGG | 1 cut(s) | 1264 |
| Eco88I | CYCGRG | 2 cut(s) | 1385, 1886 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1547 |
| EcoRII | CCWGG | 3 cut(s) | 319, 1237, 1938 |
| EcoT14I | CCWWGG | 4 cut(s) | 688, 1049, 1162, 1224 |
| EcoT22I | ATGCAT | 1 cut(s) | 1409 |
| EcoT38I | GRGCYC | 1 cut(s) | 1386 |
| ErhI | CCWWGG | 4 cut(s) | 688, 1049, 1162, 1224 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 245, 277 |
| FaqI | GGGAC | 1 cut(s) | 43 |
| FblI | GTMKAC | 2 cut(s) | 98, 1446 |
| Fnu4HI | GCNGC | 8 cut(s) | 225, 276, 714, 1068, 1098, 1590, 1614, 1743 |
| FokI | GGATG | 4 cut(s) | 632, 689, 840, 872 |
| FriOI | GRGCYC | 1 cut(s) | 1386 |
| Fsp4HI | GCNGC | 8 cut(s) | 225, 276, 714, 1068, 1098, 1590, 1614, 1743 |
| FspBI | CTAG | 5 cut(s) | 752, 1542, 1551, 1760, 1965 |
| GlaI | GCGC | 2 cut(s) | 615, 780 |
| GluI | GCNGC | 8 cut(s) | 225, 276, 714, 1068, 1098, 1590, 1614, 1743 |
| GsuI | CTGGAG | 2 cut(s) | 390, 835 |
| HaeII | RGCGCY | 2 cut(s) | 617, 782 |
| HapII | CCGG | 3 cut(s) | 717, 1596, 1887 |
| HhaI | GCGC | 2 cut(s) | 616, 781 |
| Hin6I | GCGC | 2 cut(s) | 614, 779 |
| HinP1I | GCGC | 2 cut(s) | 614, 779 |
| HpaII | CCGG | 3 cut(s) | 717, 1596, 1887 |
| HphI | GGTGA | 6 cut(s) | 560, 660, 1151, 1486, 2077, 2282 |
| Hpy166II | GTNNAC | 6 cut(s) | 99, 127, 883, 1039, 1447, 1603 |
| Hpy188I | TCNGA | 7 cut(s) | 198, 389, 484, 1189, 1297, 1454, 2223 |
| Hpy188III | TCNNGA | 9 cut(s) | 267, 407, 759, 872, 1542, 1665, 1727, 2248, 2284 |
| Hpy8I | GTNNAC | 6 cut(s) | 99, 127, 883, 1039, 1447, 1603 |
| Hpy99I | CGWCG | 1 cut(s) | 2065 |
| HpyAV | CCTTC | 6 cut(s) | 79, 494, 973, 1805, 2037, 2218 |
| HpyCH4III | ACNGT | 9 cut(s) | 991, 1063, 1330, 1368, 1498, 2065, 2077, 2149, 2270 |
| HpyCH4IV | ACGT | 1 cut(s) | 940 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 550, 710, 785, 794, 1094, 1259, 1821 |
| HpyF3I | CTNAG | 7 cut(s) | 312, 648, 793, 1000, 1264, 1481, 2220 |
| HpySE526I | ACGT | 1 cut(s) | 940 |
| HspAI | GCGC | 2 cut(s) | 614, 779 |
| KpnI | GGTACC | 1 cut(s) | 1468 |
| KroI | GCCGGC | 1 cut(s) | 716 |
| KroNI | GCCGGC | 1 cut(s) | 718 |
| Kzo9I | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| LmnI | GCTCC | 8 cut(s) | 239, 363, 611, 776, 816, 1940, 2131, 2250 |
| Lsp1109I | GCAGC | 6 cut(s) | 211, 262, 1079, 1109, 1600, 1754 |
| LweI | GCATC | 8 cut(s) | 176, 818, 826, 1165, 1328, 1964, 1996, 2284 |
| MaeI | CTAG | 5 cut(s) | 752, 1542, 1551, 1760, 1965 |
| MaeII | ACGT | 1 cut(s) | 940 |
| MaeIII | GTNAC | 7 cut(s) | 666, 1690, 1762, 1943, 2077, 2203, 2305 |
| MalI | GATC | 6 cut(s) | 162, 510, 936, 1488, 1866, 1884 |
| MboI | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| MboII | GAAGA | 3 cut(s) | 668, 2082, 2203 |
| MfeI | CAATTG | 4 cut(s) | 306, 948, 954, 1470 |
| MflI | RGATCY | 2 cut(s) | 508, 1882 |
| MhlI | GDGCHC | 3 cut(s) | 129, 595, 1386 |
| MlsI | TGGCCA | 2 cut(s) | 475, 687 |
| MluNI | TGGCCA | 2 cut(s) | 475, 687 |
| MlyI | GAGTC | 3 cut(s) | 488, 1117, 2296 |
| MmeI | TCCRAC | 1 cut(s) | 1483 |
| Mox20I | TGGCCA | 2 cut(s) | 475, 687 |
| Mph1103I | ATGCAT | 1 cut(s) | 1409 |
| MroNI | GCCGGC | 1 cut(s) | 716 |
| MroXI | GAANNNNTTC | 1 cut(s) | 660 |
| MscI | TGGCCA | 2 cut(s) | 475, 687 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1161 |
| Msp20I | TGGCCA | 2 cut(s) | 475, 687 |
| MspA1I | CMGCKG | 2 cut(s) | 713, 1004 |
| MspI | CCGG | 3 cut(s) | 717, 1596, 1887 |
| MspR9I | CCNGG | 6 cut(s) | 321, 1239, 1596, 1887, 1888, 1940 |
| MunI | CAATTG | 4 cut(s) | 306, 948, 954, 1470 |
| Mva1269I | GAATGC | 2 cut(s) | 348, 400 |
| MvaI | CCWGG | 3 cut(s) | 321, 1239, 1940 |
| MvnI | CGCG | 1 cut(s) | 2060 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 550, 710, 785, 794, 1094, 1259, 1821 |
| NaeI | GCCGGC | 1 cut(s) | 718 |
| NciI | CCSGG | 3 cut(s) | 1596, 1887, 1888 |
| NcoI | CCATGG | 3 cut(s) | 688, 1049, 1162 |
| NdeII | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| NgoMIV | GCCGGC | 1 cut(s) | 716 |
| NlaIV | GGNNCC | 6 cut(s) | 510, 1466, 1593, 1884, 2127, 2252 |
| NmuCI | GTSAC | 5 cut(s) | 666, 1690, 1762, 2077, 2305 |
| NsiI | ATGCAT | 1 cut(s) | 1409 |
| NspI | RCATGY | 1 cut(s) | 1683 |
| PaeR7I | CTCGAG | 1 cut(s) | 1385 |
| PagI | TCATGA | 1 cut(s) | 871 |
| PasI | CCCWGGG | 1 cut(s) | 1939 |
| PceI | AGGCCT | 1 cut(s) | 1176 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2308 |
| PctI | GAATGC | 2 cut(s) | 348, 400 |
| PdiI | GCCGGC | 1 cut(s) | 718 |
| PdmI | GAANNNNTTC | 1 cut(s) | 660 |
| PfeI | GAWTC | 9 cut(s) | 290, 337, 390, 461, 623, 913, 1217, 2014, 2318 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 375 |
| PfoI | TCCNGGA | 1 cut(s) | 319 |
| PkrI | GCNGC | 8 cut(s) | 226, 277, 715, 1069, 1099, 1591, 1615, 1744 |
| PleI | GAGTC | 3 cut(s) | 487, 1117, 2295 |
| PpsI | GAGTC | 3 cut(s) | 487, 1117, 2295 |
| Ppu21I | YACGTR | 1 cut(s) | 941 |
| PpuMI | RGGWCCY | 1 cut(s) | 1547 |
| Psp5II | RGGWCCY | 1 cut(s) | 1547 |
| Psp6I | CCWGG | 3 cut(s) | 319, 1237, 1938 |
| PspGI | CCWGG | 3 cut(s) | 319, 1237, 1938 |
| PspN4I | GGNNCC | 6 cut(s) | 510, 1466, 1593, 1884, 2127, 2252 |
| PspPI | GGNCC | 3 cut(s) | 1016, 1547, 1814 |
| PspPPI | RGGWCCY | 1 cut(s) | 1547 |
| PspXI | VCTCGAGB | 1 cut(s) | 1385 |
| PstI | CTGCAG | 2 cut(s) | 1744, 1912 |
| PstNI | CAGNNNCTG | 1 cut(s) | 482 |
| PsuI | RGATCY | 2 cut(s) | 508, 1882 |
| PvuII | CAGCTG | 1 cut(s) | 1004 |
| RsaI | GTAC | 4 cut(s) | 1278, 1466, 1722, 2108 |
| RsaNI | GTAC | 4 cut(s) | 1277, 1465, 1721, 2107 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1161 |
| SatI | GCNGC | 8 cut(s) | 225, 276, 714, 1068, 1098, 1590, 1614, 1743 |
| Sau3AI | GATC | 6 cut(s) | 160, 508, 934, 1486, 1864, 1882 |
| Sau96I | GGNCC | 3 cut(s) | 1016, 1547, 1814 |
| ScaI | AGTACT | 1 cut(s) | 1278 |
| SchI | GAGTC | 3 cut(s) | 488, 1117, 2296 |
| ScrFI | CCNGG | 6 cut(s) | 321, 1239, 1596, 1887, 1888, 1940 |
| SduI | GDGCHC | 3 cut(s) | 129, 595, 1386 |
| SfaNI | GCATC | 8 cut(s) | 176, 818, 826, 1165, 1328, 1964, 1996, 2284 |
| SfcI | CTRYAG | 7 cut(s) | 244, 708, 900, 1200, 1209, 1740, 1908 |
| Sfr274I | CTCGAG | 1 cut(s) | 1385 |
| SinI | GGWCC | 1 cut(s) | 1547 |
| SlaI | CTCGAG | 1 cut(s) | 1385 |
| SmaI | CCCGGG | 1 cut(s) | 1888 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1161 |
| SmlI | CTYRAG | 2 cut(s) | 294, 1385 |
| SmoI | CTYRAG | 2 cut(s) | 294, 1385 |
| SseBI | AGGCCT | 1 cut(s) | 1176 |
| SsiI | CCGC | 4 cut(s) | 713, 1589, 1625, 2058 |
| SspMI | CTAG | 5 cut(s) | 752, 1542, 1551, 1760, 1965 |
| StuI | AGGCCT | 1 cut(s) | 1176 |
| StyD4I | CCNGG | 6 cut(s) | 319, 1237, 1594, 1885, 1886, 1938 |
| StyI | CCWWGG | 4 cut(s) | 688, 1049, 1162, 1224 |
| TaaI | ACNGT | 9 cut(s) | 991, 1063, 1330, 1368, 1498, 2065, 2077, 2149, 2270 |
| TaiI | ACGT | 1 cut(s) | 943 |
| TaqI | TCGA | 4 cut(s) | 108, 933, 1386, 1621 |
| TatI | WGTACW | 3 cut(s) | 1276, 1720, 2106 |
| TauI | GCSGC | 2 cut(s) | 716, 1592 |
| TfiI | GAWTC | 9 cut(s) | 290, 337, 390, 461, 623, 913, 1217, 2014, 2318 |
| TscAI | CASTG | 6 cut(s) | 838, 1048, 1066, 1489, 1662, 2082 |
| TseFI | GTSAC | 5 cut(s) | 666, 1690, 1762, 2077, 2305 |
| TseI | GCWGC | 6 cut(s) | 224, 275, 1067, 1097, 1613, 1742 |
| Tsp45I | GTSAC | 5 cut(s) | 666, 1690, 1762, 2077, 2305 |
| TspMI | CCCGGG | 1 cut(s) | 1886 |
| TspRI | CASTG | 6 cut(s) | 838, 1048, 1066, 1489, 1662, 2082 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 375 |
| VneI | GTGCAC | 1 cut(s) | 125 |
| VpaK11BI | GGWCC | 1 cut(s) | 1547 |
| XapI | RAATTY | 4 cut(s) | 192, 534, 1398, 2187 |
| XbaI | TCTAGA | 1 cut(s) | 1541 |
| XceI | RCATGY | 1 cut(s) | 1683 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1876 |
| XhoI | CTCGAG | 1 cut(s) | 1385 |
| XmaI | CCCGGG | 1 cut(s) | 1886 |
| XmiI | GTMKAC | 2 cut(s) | 98, 1446 |
| XmnI | GAANNNNTTC | 1 cut(s) | 660 |
| XspI | CTAG | 5 cut(s) | 752, 1542, 1551, 1760, 1965 |
| ZrmI | AGTACT | 1 cut(s) | 1278 |
| Zsp2I | ATGCAT | 1 cut(s) | 1409 |