Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 740 |
| AcoI | YGGCCR | 1 cut(s) | 470 |
| AcuI | CTGAAG | 4 cut(s) | 128, 330, 362, 525 |
| AfaI | GTAC | 3 cut(s) | 307, 741, 767 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 115, 468, 755 |
| AgeI | ACCGGT | 1 cut(s) | 461 |
| AjnI | CCWGG | 1 cut(s) | 447 |
| AluBI | AGCT | 5 cut(s) | 58, 340, 532, 660, 682 |
| AluI | AGCT | 5 cut(s) | 58, 340, 532, 660, 682 |
| Alw26I | GTCTC | 1 cut(s) | 602 |
| AlwI | GGATC | 1 cut(s) | 740 |
| Ama87I | CYCGRG | 1 cut(s) | 428 |
| AoxI | GGCC | 1 cut(s) | 470 |
| ApeKI | GCWGC | 4 cut(s) | 73, 283, 346, 623 |
| AsiGI | ACCGGT | 1 cut(s) | 461 |
| AspLEI | GCGC | 1 cut(s) | 525 |
| AspS9I | GGNCC | 2 cut(s) | 238, 554 |
| AsuC2I | CCSGG | 3 cut(s) | 134, 429, 430 |
| AsuHPI | GGTGA | 2 cut(s) | 358, 382 |
| AsuII | TTCGAA | 1 cut(s) | 587 |
| AsuNHI | GCTAGC | 1 cut(s) | 194 |
| AvaI | CYCGRG | 1 cut(s) | 428 |
| AvaII | GGWCC | 2 cut(s) | 238, 554 |
| BalI | TGGCCA | 1 cut(s) | 472 |
| BanII | GRGCYC | 1 cut(s) | 435 |
| BbsI | GAAGAC | 2 cut(s) | 318, 320 |
| BbvI | GCAGC | 4 cut(s) | 85, 270, 358, 610 |
| BccI | CCATC | 2 cut(s) | 370, 505 |
| BciT130I | CCWGG | 1 cut(s) | 449 |
| BclI | TGATCA | 1 cut(s) | 387 |
| BcnI | CCSGG | 3 cut(s) | 134, 429, 430 |
| BcoDI | GTCTC | 1 cut(s) | 602 |
| BfaI | CTAG | 3 cut(s) | 171, 195, 410 |
| BisI | GCNGC | 4 cut(s) | 74, 284, 347, 624 |
| BlsI | GCNGC | 4 cut(s) | 75, 285, 348, 625 |
| BmcAI | AGTACT | 1 cut(s) | 307 |
| Bme1390I | CCNGG | 4 cut(s) | 134, 429, 430, 449 |
| Bme18I | GGWCC | 2 cut(s) | 238, 554 |
| BmeT110I | CYCGRG | 1 cut(s) | 428 |
| BmgT120I | GGNCC | 2 cut(s) | 238, 554 |
| BmiI | GGNNCC | 2 cut(s) | 555, 760 |
| BmrFI | CCNGG | 4 cut(s) | 134, 429, 430, 449 |
| BmrI | ACTGGG | 1 cut(s) | 560 |
| BmsI | GCATC | 1 cut(s) | 119 |
| BmtI | GCTAGC | 1 cut(s) | 198 |
| BmuI | ACTGGG | 1 cut(s) | 560 |
| BpiI | GAAGAC | 2 cut(s) | 318, 320 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 10, 42 |
| BpmI | CTGGAG | 1 cut(s) | 270 |
| Bpu14I | TTCGAA | 1 cut(s) | 587 |
| BpuEI | CTTGAG | 1 cut(s) | 738 |
| BpuMI | CCSGG | 3 cut(s) | 134, 429, 430 |
| BsaAI | YACGTR | 1 cut(s) | 190 |
| BsaJI | CCNNGG | 2 cut(s) | 234, 428 |
| BsaWI | WCCGGW | 1 cut(s) | 461 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 152, 182, 289, 319 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 115, 468, 755 |
| Bse118I | RCCGGY | 1 cut(s) | 461 |
| Bse1I | ACTGG | 4 cut(s) | 45, 473, 555, 606 |
| Bse3DI | GCAATG | 1 cut(s) | 356 |
| BseBI | CCWGG | 1 cut(s) | 449 |
| BseDI | CCNNGG | 2 cut(s) | 234, 428 |
| BseGI | GGATG | 2 cut(s) | 134, 405 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 115, 468, 755 |
| BseMI | GCAATG | 1 cut(s) | 356 |
| BseMII | CTCAG | 1 cut(s) | 692 |
| BseNI | ACTGG | 4 cut(s) | 45, 473, 555, 606 |
| BseXI | GCAGC | 4 cut(s) | 85, 270, 358, 610 |
| BsgI | GTGCAG | 1 cut(s) | 92 |
| BshFI | GGCC | 1 cut(s) | 472 |
| BshTI | ACCGGT | 1 cut(s) | 461 |
| BsiHKCI | CYCGRG | 1 cut(s) | 428 |
| BsiSI | CCGG | 3 cut(s) | 133, 429, 462 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 115, 468, 755 |
| BsmAI | GTCTC | 1 cut(s) | 602 |
| BsnI | GGCC | 1 cut(s) | 472 |
| BsoBI | CYCGRG | 1 cut(s) | 428 |
| Bsp119I | TTCGAA | 1 cut(s) | 587 |
| Bsp1286I | GDGCHC | 1 cut(s) | 435 |
| Bsp143I | GATC | 3 cut(s) | 387, 490, 745 |
| BspANI | GGCC | 1 cut(s) | 472 |
| BspCNI | CTCAG | 1 cut(s) | 691 |
| BspLI | GGNNCC | 2 cut(s) | 555, 760 |
| BspOI | GCTAGC | 1 cut(s) | 198 |
| BspPI | GGATC | 1 cut(s) | 740 |
| BspT104I | TTCGAA | 1 cut(s) | 587 |
| BsrDI | GCAATG | 1 cut(s) | 356 |
| BsrFI | RCCGGY | 1 cut(s) | 461 |
| BsrI | ACTGG | 4 cut(s) | 45, 473, 555, 606 |
| BssAI | RCCGGY | 1 cut(s) | 461 |
| BssECI | CCNNGG | 2 cut(s) | 234, 428 |
| BssMI | GATC | 3 cut(s) | 387, 490, 745 |
| BssT1I | CCWWGG | 1 cut(s) | 234 |
| Bst2UI | CCWGG | 1 cut(s) | 449 |
| Bst4CI | ACNGT | 1 cut(s) | 317 |
| Bst6I | CTCTTC | 1 cut(s) | 501 |
| BstBAI | YACGTR | 1 cut(s) | 190 |
| BstBI | TTCGAA | 1 cut(s) | 587 |
| BstC8I | GCNNGC | 1 cut(s) | 196 |
| BstDEI | CTNAG | 1 cut(s) | 678 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 113, 753 |
| BstF5I | GGATG | 2 cut(s) | 134, 405 |
| BstHHI | GCGC | 1 cut(s) | 525 |
| BstKTI | GATC | 3 cut(s) | 390, 493, 748 |
| BstMAI | GTCTC | 1 cut(s) | 602 |
| BstMBI | GATC | 3 cut(s) | 387, 490, 745 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 346, 529 |
| BstNI | CCWGG | 1 cut(s) | 449 |
| BstNSI | RCATGY | 1 cut(s) | 215 |
| BstSCI | CCNGG | 4 cut(s) | 132, 427, 428, 447 |
| BstV1I | GCAGC | 4 cut(s) | 85, 270, 358, 610 |
| BstV2I | GAAGAC | 2 cut(s) | 318, 320 |
| BstX2I | RGATCY | 1 cut(s) | 745 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 248, 601 |
| BstYI | RGATCY | 1 cut(s) | 745 |
| BsuRI | GGCC | 1 cut(s) | 472 |
| BtsCI | GGATG | 2 cut(s) | 134, 405 |
| BtsIMutI | CAGTG | 2 cut(s) | 52, 548 |
| Cac8I | GCNNGC | 1 cut(s) | 196 |
| CfoI | GCGC | 1 cut(s) | 525 |
| Cfr10I | RCCGGY | 1 cut(s) | 461 |
| Cfr13I | GGNCC | 2 cut(s) | 238, 554 |
| Cfr9I | CCCGGG | 1 cut(s) | 428 |
| Csp6I | GTAC | 3 cut(s) | 306, 740, 766 |
| CspAI | ACCGGT | 1 cut(s) | 461 |
| CviAII | CATG | 4 cut(s) | 77, 212, 537, 730 |
| CviQI | GTAC | 3 cut(s) | 306, 740, 766 |
| DdeI | CTNAG | 1 cut(s) | 678 |
| DpnI | GATC | 3 cut(s) | 389, 492, 747 |
| DpnII | GATC | 3 cut(s) | 387, 490, 745 |
| EaeI | YGGCCR | 1 cut(s) | 470 |
| Eam1104I | CTCTTC | 1 cut(s) | 501 |
| EarI | CTCTTC | 1 cut(s) | 501 |
| Eco130I | CCWWGG | 1 cut(s) | 234 |
| Eco24I | GRGCYC | 1 cut(s) | 435 |
| Eco47I | GGWCC | 2 cut(s) | 238, 554 |
| Eco57I | CTGAAG | 4 cut(s) | 128, 330, 362, 525 |
| Eco88I | CYCGRG | 1 cut(s) | 428 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 113, 753 |
| EcoO109I | RGGNCCY | 1 cut(s) | 554 |
| EcoRII | CCWGG | 1 cut(s) | 447 |
| EcoT14I | CCWWGG | 1 cut(s) | 234 |
| EcoT38I | GRGCYC | 1 cut(s) | 435 |
| ErhI | CCWWGG | 1 cut(s) | 234 |
| FaeI | CATG | 4 cut(s) | 80, 215, 540, 733 |
| FaiI | YATR | 9 cut(s) | 78, 96, 178, 180, 213, 538, 544, 596, 731 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 644, 676 |
| FatI | CATG | 4 cut(s) | 76, 211, 536, 729 |
| FbaI | TGATCA | 1 cut(s) | 387 |
| Fnu4HI | GCNGC | 4 cut(s) | 74, 284, 347, 624 |
| FokI | GGATG | 2 cut(s) | 141, 392 |
| FriOI | GRGCYC | 1 cut(s) | 435 |
| Fsp4HI | GCNGC | 4 cut(s) | 74, 284, 347, 624 |
| FspBI | CTAG | 3 cut(s) | 171, 195, 410 |
| GlaI | GCGC | 1 cut(s) | 524 |
| GluI | GCNGC | 4 cut(s) | 74, 284, 347, 624 |
| GsuI | CTGGAG | 1 cut(s) | 270 |
| HaeIII | GGCC | 1 cut(s) | 472 |
| HapII | CCGG | 3 cut(s) | 133, 429, 462 |
| HhaI | GCGC | 1 cut(s) | 525 |
| Hin1II | CATG | 4 cut(s) | 80, 215, 540, 733 |
| Hin6I | GCGC | 1 cut(s) | 523 |
| HinP1I | GCGC | 1 cut(s) | 523 |
| HinfI | GANTC | 3 cut(s) | 122, 418, 584 |
| HpaII | CCGG | 3 cut(s) | 133, 429, 462 |
| HphI | GGTGA | 2 cut(s) | 358, 382 |
| Hpy166II | GTNNAC | 1 cut(s) | 359 |
| Hpy188I | TCNGA | 6 cut(s) | 147, 206, 297, 333, 490, 667 |
| Hpy188III | TCNNGA | 2 cut(s) | 126, 503 |
| Hpy8I | GTNNAC | 1 cut(s) | 359 |
| HpyAV | CCTTC | 5 cut(s) | 218, 374, 509, 567, 759 |
| HpyCH4III | ACNGT | 1 cut(s) | 317 |
| HpyCH4IV | ACGT | 2 cut(s) | 189, 564 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 73, 261 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 346, 529 |
| HpyF3I | CTNAG | 1 cut(s) | 678 |
| HpySE526I | ACGT | 2 cut(s) | 189, 564 |
| Hsp92II | CATG | 4 cut(s) | 80, 215, 540, 733 |
| HspAI | GCGC | 1 cut(s) | 523 |
| Ksp22I | TGATCA | 1 cut(s) | 387 |
| Kzo9I | GATC | 3 cut(s) | 387, 490, 745 |
| LmnI | GCTCC | 1 cut(s) | 758 |
| Lsp1109I | GCAGC | 4 cut(s) | 85, 270, 358, 610 |
| LweI | GCATC | 1 cut(s) | 119 |
| MaeI | CTAG | 3 cut(s) | 171, 195, 410 |
| MaeII | ACGT | 2 cut(s) | 189, 564 |
| MaeIII | GTNAC | 3 cut(s) | 214, 464, 476 |
| MalI | GATC | 3 cut(s) | 389, 492, 747 |
| MboI | GATC | 3 cut(s) | 387, 490, 745 |
| MboII | GAAGA | 3 cut(s) | 320, 323, 518 |
| MflI | RGATCY | 1 cut(s) | 745 |
| MhlI | GDGCHC | 1 cut(s) | 435 |
| MlsI | TGGCCA | 1 cut(s) | 472 |
| MluCI | AATT | 2 cut(s) | 362, 436 |
| MluNI | TGGCCA | 1 cut(s) | 472 |
| MmeI | TCCRAC | 3 cut(s) | 42, 265, 585 |
| MnlI | CCTC | 7 cut(s) | 6, 12, 112, 154, 245, 379, 565 |
| Mox20I | TGGCCA | 1 cut(s) | 472 |
| MscI | TGGCCA | 1 cut(s) | 472 |
| MseI | TTAA | 1 cut(s) | 639 |
| Msp20I | TGGCCA | 1 cut(s) | 472 |
| MspI | CCGG | 3 cut(s) | 133, 429, 462 |
| MspR9I | CCNGG | 4 cut(s) | 134, 429, 430, 449 |
| MvaI | CCWGG | 1 cut(s) | 449 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 346, 529 |
| NciI | CCSGG | 3 cut(s) | 134, 429, 430 |
| NdeII | GATC | 3 cut(s) | 387, 490, 745 |
| NheI | GCTAGC | 1 cut(s) | 194 |
| NlaIII | CATG | 4 cut(s) | 80, 215, 540, 733 |
| NlaIV | GGNNCC | 2 cut(s) | 555, 760 |
| NmeAIII | GCCGAG | 1 cut(s) | 673 |
| NspI | RCATGY | 1 cut(s) | 215 |
| NspV | TTCGAA | 1 cut(s) | 587 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 195 |
| PfeI | GAWTC | 3 cut(s) | 122, 418, 584 |
| PinAI | ACCGGT | 1 cut(s) | 461 |
| PkrI | GCNGC | 4 cut(s) | 75, 285, 348, 625 |
| Ppu21I | YACGTR | 1 cut(s) | 190 |
| PpuMI | RGGWCCY | 1 cut(s) | 554 |
| Psp5II | RGGWCCY | 1 cut(s) | 554 |
| Psp6I | CCWGG | 1 cut(s) | 447 |
| PspGI | CCWGG | 1 cut(s) | 447 |
| PspN4I | GGNNCC | 2 cut(s) | 555, 760 |
| PspPI | GGNCC | 2 cut(s) | 238, 554 |
| PspPPI | RGGWCCY | 1 cut(s) | 554 |
| PsuI | RGATCY | 1 cut(s) | 745 |
| RsaI | GTAC | 3 cut(s) | 307, 741, 767 |
| RsaNI | GTAC | 3 cut(s) | 306, 740, 766 |
| SaqAI | TTAA | 1 cut(s) | 639 |
| SatI | GCNGC | 4 cut(s) | 74, 284, 347, 624 |
| Sau3AI | GATC | 3 cut(s) | 387, 490, 745 |
| Sau96I | GGNCC | 2 cut(s) | 238, 554 |
| ScaI | AGTACT | 1 cut(s) | 307 |
| ScrFI | CCNGG | 4 cut(s) | 134, 429, 430, 449 |
| SduI | GDGCHC | 1 cut(s) | 435 |
| SfaNI | GCATC | 1 cut(s) | 119 |
| SfuI | TTCGAA | 1 cut(s) | 587 |
| SinI | GGWCC | 2 cut(s) | 238, 554 |
| SmaI | CCCGGG | 1 cut(s) | 430 |
| SmlI | CTYRAG | 1 cut(s) | 753 |
| SmoI | CTYRAG | 1 cut(s) | 753 |
| Sse9I | AATT | 2 cut(s) | 362, 436 |
| SspMI | CTAG | 3 cut(s) | 171, 195, 410 |
| StyD4I | CCNGG | 4 cut(s) | 132, 427, 428, 447 |
| StyI | CCWWGG | 1 cut(s) | 234 |
| TaaI | ACNGT | 1 cut(s) | 317 |
| TaiI | ACGT | 2 cut(s) | 192, 567 |
| TaqI | TCGA | 4 cut(s) | 41, 421, 570, 587 |
| TasI | AATT | 2 cut(s) | 362, 436 |
| TatI | WGTACW | 3 cut(s) | 305, 739, 765 |
| TfiI | GAWTC | 3 cut(s) | 122, 418, 584 |
| Tru1I | TTAA | 1 cut(s) | 639 |
| Tru9I | TTAA | 1 cut(s) | 639 |
| TscAI | CASTG | 2 cut(s) | 52, 555 |
| TseI | GCWGC | 4 cut(s) | 73, 283, 346, 623 |
| TspDTI | ATGAA | 5 cut(s) | 237, 280, 394, 708, 718 |
| TspMI | CCCGGG | 1 cut(s) | 428 |
| TspRI | CASTG | 2 cut(s) | 52, 555 |
| VpaK11BI | GGWCC | 2 cut(s) | 238, 554 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 113, 753 |
| XceI | RCATGY | 1 cut(s) | 215 |
| XmaI | CCCGGG | 1 cut(s) | 428 |
| XspI | CTAG | 3 cut(s) | 171, 195, 410 |
| ZrmI | AGTACT | 1 cut(s) | 307 |