Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 784 |
| Acc36I | ACCTGC | 1 cut(s) | 784 |
| Acc65I | GGTACC | 1 cut(s) | 241 |
| AccB1I | GGYRCC | 1 cut(s) | 241 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 601 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 983 |
| AcuI | CTGAAG | 2 cut(s) | 131, 454 |
| AcyI | GRCGYC | 1 cut(s) | 45 |
| AfaI | GTAC | 5 cut(s) | 68, 243, 323, 815, 950 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 396, 471, 559, 601, 727 |
| AflIII | ACRYGT | 1 cut(s) | 264 |
| AgsI | TTSAA | 4 cut(s) | 63, 339, 367, 610 |
| AhlI | ACTAGT | 1 cut(s) | 359 |
| AjnI | CCWGG | 1 cut(s) | 706 |
| AluBI | AGCT | 6 cut(s) | 19, 200, 546, 578, 836, 922 |
| AluI | AGCT | 6 cut(s) | 19, 200, 546, 578, 836, 922 |
| Alw21I | GWGCWC | 1 cut(s) | 924 |
| AoxI | GGCC | 6 cut(s) | 3, 74, 151, 449, 531, 550 |
| ApeKI | GCWGC | 3 cut(s) | 11, 826, 1014 |
| ApoI | RAATTY | 1 cut(s) | 983 |
| Asp718I | GGTACC | 1 cut(s) | 241 |
| AspS9I | GGNCC | 5 cut(s) | 75, 151, 161, 550, 908 |
| AsuC2I | CCSGG | 1 cut(s) | 657 |
| AsuHPI | GGTGA | 4 cut(s) | 673, 794, 808, 882 |
| AsuII | TTCGAA | 1 cut(s) | 724 |
| AvaII | GGWCC | 2 cut(s) | 161, 908 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 805, 838 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BanI | GGYRCC | 1 cut(s) | 241 |
| BanII | GRGCYC | 2 cut(s) | 128, 924 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 941, 973 |
| Bbv12I | GWGCWC | 1 cut(s) | 924 |
| BbvI | GCAGC | 3 cut(s) | 23, 813, 1001 |
| BccI | CCATC | 4 cut(s) | 86, 137, 206, 895 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 704, 738 |
| BciT130I | CCWGG | 1 cut(s) | 708 |
| BclI | TGATCA | 2 cut(s) | 784, 934 |
| BcnI | CCSGG | 1 cut(s) | 657 |
| BcuI | ACTAGT | 1 cut(s) | 359 |
| BfaI | CTAG | 1 cut(s) | 360 |
| BfuAI | ACCTGC | 1 cut(s) | 784 |
| BglII | AGATCT | 1 cut(s) | 637 |
| BisI | GCNGC | 3 cut(s) | 12, 827, 1015 |
| BlsI | GCNGC | 3 cut(s) | 13, 828, 1016 |
| Bme1390I | CCNGG | 2 cut(s) | 657, 708 |
| Bme18I | GGWCC | 2 cut(s) | 161, 908 |
| BmgT120I | GGNCC | 5 cut(s) | 75, 151, 161, 550, 908 |
| BmiI | GGNNCC | 4 cut(s) | 127, 163, 243, 413 |
| BmrFI | CCNGG | 2 cut(s) | 657, 708 |
| BmsI | GCATC | 1 cut(s) | 886 |
| BpmI | CTGGAG | 1 cut(s) | 415 |
| Bpu14I | TTCGAA | 1 cut(s) | 724 |
| BpuEI | CTTGAG | 1 cut(s) | 548 |
| BpuMI | CCSGG | 1 cut(s) | 657 |
| Bsa29I | ATCGAT | 1 cut(s) | 258 |
| BsaHI | GRCGYC | 1 cut(s) | 45 |
| BsaJI | CCNNGG | 1 cut(s) | 915 |
| BsaWI | WCCGGW | 1 cut(s) | 244 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 23, 53 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 396, 471, 559, 601, 727 |
| Bse1I | ACTGG | 1 cut(s) | 398 |
| Bse3DI | GCAATG | 2 cut(s) | 811, 951 |
| BseBI | CCWGG | 1 cut(s) | 708 |
| BseCI | ATCGAT | 1 cut(s) | 258 |
| BseDI | CCNNGG | 1 cut(s) | 915 |
| BseGI | GGATG | 2 cut(s) | 129, 318 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 396, 471, 559, 601, 727 |
| BseMI | GCAATG | 2 cut(s) | 811, 951 |
| BseMII | CTCAG | 1 cut(s) | 421 |
| BseNI | ACTGG | 1 cut(s) | 398 |
| BseRI | GAGGAG | 1 cut(s) | 263 |
| BseXI | GCAGC | 3 cut(s) | 23, 813, 1001 |
| BsgI | GTGCAG | 1 cut(s) | 812 |
| Bsh1285I | CGRYCG | 1 cut(s) | 88 |
| BshFI | GGCC | 6 cut(s) | 5, 76, 153, 451, 533, 552 |
| BshNI | GGYRCC | 1 cut(s) | 241 |
| BshVI | ATCGAT | 1 cut(s) | 258 |
| BsiEI | CGRYCG | 1 cut(s) | 88 |
| BsiHKAI | GWGCWC | 1 cut(s) | 924 |
| BsiSI | CCGG | 4 cut(s) | 42, 245, 299, 656 |
| BslFI | GGGAC | 5 cut(s) | 41, 147, 413, 672, 1004 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 396, 471, 559, 601, 727 |
| BsmFI | GGGAC | 5 cut(s) | 41, 147, 413, 672, 1004 |
| BsnI | GGCC | 6 cut(s) | 5, 76, 153, 451, 533, 552 |
| Bsp119I | TTCGAA | 1 cut(s) | 724 |
| Bsp1286I | GDGCHC | 2 cut(s) | 128, 924 |
| Bsp1407I | TGTACA | 1 cut(s) | 948 |
| Bsp143I | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| BspANI | GGCC | 6 cut(s) | 5, 76, 153, 451, 533, 552 |
| BspCNI | CTCAG | 1 cut(s) | 420 |
| BspDI | ATCGAT | 1 cut(s) | 258 |
| BspLI | GGNNCC | 4 cut(s) | 127, 163, 243, 413 |
| BspMI | ACCTGC | 1 cut(s) | 784 |
| BspT104I | TTCGAA | 1 cut(s) | 724 |
| BspT107I | GGYRCC | 1 cut(s) | 241 |
| BsrDI | GCAATG | 2 cut(s) | 811, 951 |
| BsrGI | TGTACA | 1 cut(s) | 948 |
| BsrI | ACTGG | 1 cut(s) | 398 |
| BssECI | CCNNGG | 1 cut(s) | 915 |
| BssMI | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| BssNI | GRCGYC | 1 cut(s) | 45 |
| BssT1I | CCWWGG | 1 cut(s) | 915 |
| Bst2UI | CCWGG | 1 cut(s) | 708 |
| Bst4CI | ACNGT | 5 cut(s) | 89, 161, 419, 671, 884 |
| BstACI | GRCGYC | 1 cut(s) | 45 |
| BstAUI | TGTACA | 1 cut(s) | 948 |
| BstBI | TTCGAA | 1 cut(s) | 724 |
| BstC8I | GCNNGC | 5 cut(s) | 202, 554, 587, 591, 653 |
| BstDEI | CTNAG | 2 cut(s) | 407, 1018 |
| BstEII | GGTNACC | 1 cut(s) | 671 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 469, 557, 725 |
| BstF5I | GGATG | 2 cut(s) | 129, 318 |
| BstKTI | GATC | 5 cut(s) | 145, 258, 640, 787, 937 |
| BstMBI | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| BstMCI | CGRYCG | 1 cut(s) | 88 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 11, 712 |
| BstNI | CCWGG | 1 cut(s) | 708 |
| BstNSI | RCATGY | 1 cut(s) | 268 |
| BstPI | GGTNACC | 1 cut(s) | 671 |
| BstSCI | CCNGG | 2 cut(s) | 655, 706 |
| BstV1I | GCAGC | 3 cut(s) | 23, 813, 1001 |
| BstX2I | RGATCY | 1 cut(s) | 637 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 212 |
| BstYI | RGATCY | 1 cut(s) | 637 |
| Bsu15I | ATCGAT | 1 cut(s) | 258 |
| BsuRI | GGCC | 6 cut(s) | 5, 76, 153, 451, 533, 552 |
| BsuTUI | ATCGAT | 1 cut(s) | 258 |
| BtsCI | GGATG | 2 cut(s) | 129, 318 |
| BtsIMutI | CAGTG | 1 cut(s) | 415 |
| BveI | ACCTGC | 1 cut(s) | 784 |
| Cac8I | GCNNGC | 5 cut(s) | 202, 554, 587, 591, 653 |
| Cfr13I | GGNCC | 5 cut(s) | 75, 151, 161, 550, 908 |
| ClaI | ATCGAT | 1 cut(s) | 258 |
| CseI | GACGC | 1 cut(s) | 34 |
| Csp6I | GTAC | 5 cut(s) | 67, 242, 322, 814, 949 |
| CviAII | CATG | 3 cut(s) | 236, 265, 932 |
| CviQI | GTAC | 5 cut(s) | 67, 242, 322, 814, 949 |
| DdeI | CTNAG | 2 cut(s) | 407, 1018 |
| DpnI | GATC | 5 cut(s) | 144, 257, 639, 786, 936 |
| DpnII | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Ecl136II | GAGCTC | 1 cut(s) | 922 |
| Eco130I | CCWWGG | 1 cut(s) | 915 |
| Eco24I | GRGCYC | 2 cut(s) | 128, 924 |
| Eco47I | GGWCC | 2 cut(s) | 161, 908 |
| Eco53kI | GAGCTC | 1 cut(s) | 922 |
| Eco57I | CTGAAG | 2 cut(s) | 131, 454 |
| Eco91I | GGTNACC | 1 cut(s) | 671 |
| EcoICRI | GAGCTC | 1 cut(s) | 922 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 469, 557, 725 |
| EcoO109I | RGGNCCY | 1 cut(s) | 151 |
| EcoO65I | GGTNACC | 1 cut(s) | 671 |
| EcoRII | CCWGG | 1 cut(s) | 706 |
| EcoT14I | CCWWGG | 1 cut(s) | 915 |
| EcoT22I | ATGCAT | 1 cut(s) | 901 |
| EcoT38I | GRGCYC | 2 cut(s) | 128, 924 |
| ErhI | CCWWGG | 1 cut(s) | 915 |
| FaeI | CATG | 3 cut(s) | 239, 268, 935 |
| FaqI | GGGAC | 5 cut(s) | 41, 147, 413, 672, 1004 |
| FatI | CATG | 3 cut(s) | 235, 264, 931 |
| FauNDI | CATATG | 2 cut(s) | 600, 964 |
| FbaI | TGATCA | 2 cut(s) | 784, 934 |
| Fnu4HI | GCNGC | 3 cut(s) | 12, 827, 1015 |
| FokI | GGATG | 2 cut(s) | 116, 325 |
| FriOI | GRGCYC | 2 cut(s) | 128, 924 |
| Fsp4HI | GCNGC | 3 cut(s) | 12, 827, 1015 |
| FspBI | CTAG | 1 cut(s) | 360 |
| GluI | GCNGC | 3 cut(s) | 12, 827, 1015 |
| GsuI | CTGGAG | 1 cut(s) | 415 |
| HaeIII | GGCC | 6 cut(s) | 5, 76, 153, 451, 533, 552 |
| HapII | CCGG | 4 cut(s) | 42, 245, 299, 656 |
| HgaI | GACGC | 1 cut(s) | 34 |
| Hin1I | GRCGYC | 1 cut(s) | 45 |
| Hin1II | CATG | 3 cut(s) | 239, 268, 935 |
| HincII | GTYRAC | 1 cut(s) | 49 |
| HindII | GTYRAC | 1 cut(s) | 49 |
| HindIII | AAGCTT | 2 cut(s) | 198, 576 |
| HinfI | GANTC | 1 cut(s) | 510 |
| HpaII | CCGG | 4 cut(s) | 42, 245, 299, 656 |
| HphI | GGTGA | 4 cut(s) | 673, 794, 808, 882 |
| Hpy166II | GTNNAC | 4 cut(s) | 49, 442, 748, 844 |
| Hpy188I | TCNGA | 5 cut(s) | 85, 121, 175, 328, 541 |
| Hpy188III | TCNNGA | 4 cut(s) | 373, 428, 443, 629 |
| Hpy8I | GTNNAC | 4 cut(s) | 49, 442, 748, 844 |
| Hpy99I | CGWCG | 2 cut(s) | 107, 671 |
| HpyAV | CCTTC | 5 cut(s) | 69, 345, 478, 731, 886 |
| HpyCH4III | ACNGT | 5 cut(s) | 89, 161, 419, 671, 884 |
| HpyCH4IV | ACGT | 1 cut(s) | 105 |
| HpyCH4V | TGCA | 8 cut(s) | 495, 556, 715, 793, 829, 849, 899, 1004 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 11, 712 |
| HpyF3I | CTNAG | 2 cut(s) | 407, 1018 |
| HpySE526I | ACGT | 1 cut(s) | 105 |
| Hsp92I | GRCGYC | 1 cut(s) | 45 |
| Hsp92II | CATG | 3 cut(s) | 239, 268, 935 |
| KpnI | GGTACC | 1 cut(s) | 245 |
| Ksp22I | TGATCA | 2 cut(s) | 784, 934 |
| Kzo9I | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| LmnI | GCTCC | 3 cut(s) | 131, 417, 919 |
| Lsp1109I | GCAGC | 3 cut(s) | 23, 813, 1001 |
| LweI | GCATC | 1 cut(s) | 886 |
| MaeI | CTAG | 1 cut(s) | 360 |
| MaeII | ACGT | 1 cut(s) | 105 |
| MaeIII | GTNAC | 2 cut(s) | 419, 671 |
| MalI | GATC | 5 cut(s) | 144, 257, 639, 786, 936 |
| MboI | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| MboII | GAAGA | 3 cut(s) | 137, 328, 443 |
| MflI | RGATCY | 1 cut(s) | 637 |
| MhlI | GDGCHC | 2 cut(s) | 128, 924 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 4 cut(s) | 610, 867, 983, 1036 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 504 |
| MmeI | TCCRAC | 2 cut(s) | 108, 896 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| Mph1103I | ATGCAT | 1 cut(s) | 901 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 3 cut(s) | 228, 269, 518 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspI | CCGG | 4 cut(s) | 42, 245, 299, 656 |
| MspR9I | CCNGG | 2 cut(s) | 657, 708 |
| MvaI | CCWGG | 1 cut(s) | 708 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 11, 712 |
| NciI | CCSGG | 1 cut(s) | 657 |
| NdeI | CATATG | 2 cut(s) | 600, 964 |
| NdeII | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| NlaIII | CATG | 3 cut(s) | 239, 268, 935 |
| NlaIV | GGNNCC | 4 cut(s) | 127, 163, 243, 413 |
| NmeAIII | GCCGAG | 1 cut(s) | 994 |
| NsiI | ATGCAT | 1 cut(s) | 901 |
| NspI | RCATGY | 1 cut(s) | 268 |
| NspV | TTCGAA | 1 cut(s) | 724 |
| PaqCI | CACCTGC | 1 cut(s) | 784 |
| PciI | ACATGT | 1 cut(s) | 264 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 601 |
| PkrI | GCNGC | 3 cut(s) | 13, 828, 1016 |
| PleI | GAGTC | 1 cut(s) | 504 |
| PpsI | GAGTC | 1 cut(s) | 504 |
| PscI | ACATGT | 1 cut(s) | 264 |
| Psp124BI | GAGCTC | 1 cut(s) | 924 |
| Psp6I | CCWGG | 1 cut(s) | 706 |
| PspEI | GGTNACC | 1 cut(s) | 671 |
| PspGI | CCWGG | 1 cut(s) | 706 |
| PspN4I | GGNNCC | 4 cut(s) | 127, 163, 243, 413 |
| PspPI | GGNCC | 5 cut(s) | 75, 151, 161, 550, 908 |
| PsuI | RGATCY | 1 cut(s) | 637 |
| RsaI | GTAC | 5 cut(s) | 68, 243, 323, 815, 950 |
| RsaNI | GTAC | 5 cut(s) | 67, 242, 322, 814, 949 |
| SacI | GAGCTC | 1 cut(s) | 924 |
| SaqAI | TTAA | 3 cut(s) | 228, 269, 518 |
| SatI | GCNGC | 3 cut(s) | 12, 827, 1015 |
| Sau3AI | GATC | 5 cut(s) | 142, 255, 637, 784, 934 |
| Sau96I | GGNCC | 5 cut(s) | 75, 151, 161, 550, 908 |
| SchI | GAGTC | 1 cut(s) | 504 |
| ScrFI | CCNGG | 2 cut(s) | 657, 708 |
| SduI | GDGCHC | 2 cut(s) | 128, 924 |
| SfaNI | GCATC | 1 cut(s) | 886 |
| SfuI | TTCGAA | 1 cut(s) | 724 |
| SinI | GGWCC | 2 cut(s) | 161, 908 |
| SmlI | CTYRAG | 1 cut(s) | 527 |
| SmoI | CTYRAG | 1 cut(s) | 527 |
| SpeI | ACTAGT | 1 cut(s) | 359 |
| Sse9I | AATT | 4 cut(s) | 610, 867, 983, 1036 |
| SspI | AATATT | 1 cut(s) | 778 |
| SspMI | CTAG | 1 cut(s) | 360 |
| SstI | GAGCTC | 1 cut(s) | 924 |
| StyD4I | CCNGG | 2 cut(s) | 655, 706 |
| StyI | CCWWGG | 1 cut(s) | 915 |
| TaaI | ACNGT | 5 cut(s) | 89, 161, 419, 671, 884 |
| TaiI | ACGT | 1 cut(s) | 108 |
| TaqI | TCGA | 2 cut(s) | 258, 724 |
| TasI | AATT | 4 cut(s) | 610, 867, 983, 1036 |
| TatI | WGTACW | 3 cut(s) | 66, 321, 948 |
| Tru1I | TTAA | 3 cut(s) | 228, 269, 518 |
| Tru9I | TTAA | 3 cut(s) | 228, 269, 518 |
| TscAI | CASTG | 1 cut(s) | 422 |
| TseI | GCWGC | 3 cut(s) | 11, 826, 1014 |
| TspDTI | ATGAA | 2 cut(s) | 329, 757 |
| TspRI | CASTG | 1 cut(s) | 422 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 601 |
| VpaK11BI | GGWCC | 2 cut(s) | 161, 908 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 469, 557, 725 |
| XapI | RAATTY | 1 cut(s) | 983 |
| XceI | RCATGY | 1 cut(s) | 268 |
| XspI | CTAG | 1 cut(s) | 360 |
| Zsp2I | ATGCAT | 1 cut(s) | 901 |