Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 525 |
| AccII | CGCG | 1 cut(s) | 282 |
| AclWI | GGATC | 1 cut(s) | 28 |
| AcsI | RAATTY | 1 cut(s) | 662 |
| AcuI | CTGAAG | 2 cut(s) | 332, 723 |
| AfaI | GTAC | 5 cut(s) | 218, 258, 410, 446, 622 |
| AflIII | ACRYGT | 2 cut(s) | 81, 462 |
| AgsI | TTSAA | 5 cut(s) | 92, 165, 338, 344, 469 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 548, 580 |
| AluBI | AGCT | 3 cut(s) | 121, 541, 652 |
| AluI | AGCT | 3 cut(s) | 121, 541, 652 |
| Alw21I | GWGCWC | 1 cut(s) | 307 |
| Alw26I | GTCTC | 2 cut(s) | 542, 700 |
| AlwI | GGATC | 1 cut(s) | 28 |
| ApeKI | GCWGC | 2 cut(s) | 118, 175 |
| ApoI | RAATTY | 1 cut(s) | 662 |
| AsuHPI | GGTGA | 2 cut(s) | 31, 702 |
| AsuII | TTCGAA | 1 cut(s) | 378 |
| BbsI | GAAGAC | 1 cut(s) | 146 |
| Bbv12I | GWGCWC | 1 cut(s) | 307 |
| BbvI | GCAGC | 2 cut(s) | 130, 162 |
| BcoDI | GTCTC | 2 cut(s) | 542, 700 |
| BfaI | CTAG | 1 cut(s) | 657 |
| BfuAI | ACCTGC | 1 cut(s) | 525 |
| BisI | GCNGC | 2 cut(s) | 119, 176 |
| BlsI | GCNGC | 2 cut(s) | 120, 177 |
| BmcAI | AGTACT | 2 cut(s) | 410, 446 |
| BmsI | GCATC | 2 cut(s) | 349, 434 |
| BpiI | GAAGAC | 1 cut(s) | 146 |
| BpmI | CTGGAG | 1 cut(s) | 665 |
| Bpu14I | TTCGAA | 1 cut(s) | 378 |
| BpuEI | CTTGAG | 1 cut(s) | 505 |
| BsaBI | GATNNNNATC | 1 cut(s) | 639 |
| Bse3DI | GCAATG | 2 cut(s) | 559, 747 |
| Bse8I | GATNNNNATC | 1 cut(s) | 639 |
| BseGI | GGATG | 3 cut(s) | 364, 623, 640 |
| BseJI | GATNNNNATC | 1 cut(s) | 639 |
| BseMI | GCAATG | 2 cut(s) | 559, 747 |
| BseMII | CTCAG | 3 cut(s) | 47, 57, 128 |
| BseRI | GAGGAG | 2 cut(s) | 90, 662 |
| BseXI | GCAGC | 2 cut(s) | 130, 162 |
| Bsh1236I | CGCG | 1 cut(s) | 282 |
| BsiHKAI | GWGCWC | 1 cut(s) | 307 |
| BsmAI | GTCTC | 2 cut(s) | 542, 700 |
| BsmI | GAATGC | 2 cut(s) | 485, 604 |
| Bsp119I | TTCGAA | 1 cut(s) | 378 |
| Bsp1286I | GDGCHC | 1 cut(s) | 307 |
| Bsp143I | GATC | 1 cut(s) | 20 |
| BspCNI | CTCAG | 3 cut(s) | 46, 56, 127 |
| BspFNI | CGCG | 1 cut(s) | 282 |
| BspMI | ACCTGC | 1 cut(s) | 525 |
| BspPI | GGATC | 1 cut(s) | 28 |
| BspT104I | TTCGAA | 1 cut(s) | 378 |
| BsrDI | GCAATG | 2 cut(s) | 559, 747 |
| BssMI | GATC | 1 cut(s) | 20 |
| Bst4CI | ACNGT | 2 cut(s) | 169, 553 |
| BstBI | TTCGAA | 1 cut(s) | 378 |
| BstDEI | CTNAG | 3 cut(s) | 33, 43, 114 |
| BstF5I | GGATG | 3 cut(s) | 364, 623, 640 |
| BstFNI | CGCG | 1 cut(s) | 282 |
| BstKTI | GATC | 1 cut(s) | 23 |
| BstMAI | GTCTC | 2 cut(s) | 542, 700 |
| BstMBI | GATC | 1 cut(s) | 20 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 677 |
| BstNSI | RCATGY | 3 cut(s) | 85, 391, 466 |
| BstUI | CGCG | 1 cut(s) | 282 |
| BstV1I | GCAGC | 2 cut(s) | 130, 162 |
| BstV2I | GAAGAC | 1 cut(s) | 146 |
| BtsCI | GGATG | 3 cut(s) | 364, 623, 640 |
| BtsIMutI | CAGTG | 2 cut(s) | 174, 558 |
| BveI | ACCTGC | 1 cut(s) | 525 |
| Csp6I | GTAC | 5 cut(s) | 217, 257, 409, 445, 621 |
| CviAII | CATG | 5 cut(s) | 82, 194, 388, 463, 578 |
| CviJI | RGCY | 4 cut(s) | 60, 121, 541, 652 |
| CviKI_1 | RGCY | 4 cut(s) | 60, 121, 541, 652 |
| CviQI | GTAC | 5 cut(s) | 217, 257, 409, 445, 621 |
| DdeI | CTNAG | 3 cut(s) | 33, 43, 114 |
| DpnI | GATC | 1 cut(s) | 22 |
| DpnII | GATC | 1 cut(s) | 20 |
| Eco57I | CTGAAG | 2 cut(s) | 332, 723 |
| EcoT22I | ATGCAT | 1 cut(s) | 273 |
| FaeI | CATG | 5 cut(s) | 85, 197, 391, 466, 581 |
| FatI | CATG | 5 cut(s) | 81, 193, 387, 462, 577 |
| Fnu4HI | GCNGC | 2 cut(s) | 119, 176 |
| FokI | GGATG | 3 cut(s) | 371, 630, 647 |
| Fsp4HI | GCNGC | 2 cut(s) | 119, 176 |
| FspBI | CTAG | 1 cut(s) | 657 |
| GluI | GCNGC | 2 cut(s) | 119, 176 |
| GsuI | CTGGAG | 1 cut(s) | 665 |
| Hin1II | CATG | 5 cut(s) | 85, 197, 391, 466, 581 |
| HinfI | GANTC | 2 cut(s) | 592, 693 |
| HphI | GGTGA | 2 cut(s) | 31, 702 |
| Hpy166II | GTNNAC | 2 cut(s) | 39, 710 |
| Hpy188I | TCNGA | 6 cut(s) | 28, 46, 138, 525, 534, 597 |
| Hpy188III | TCNNGA | 2 cut(s) | 615, 644 |
| Hpy8I | GTNNAC | 2 cut(s) | 39, 710 |
| HpyAV | CCTTC | 3 cut(s) | 463, 483, 698 |
| HpyCH4III | ACNGT | 2 cut(s) | 169, 553 |
| HpyCH4IV | ACGT | 1 cut(s) | 219 |
| HpyCH4V | TGCA | 3 cut(s) | 211, 271, 418 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 677 |
| HpyF3I | CTNAG | 3 cut(s) | 33, 43, 114 |
| HpySE526I | ACGT | 1 cut(s) | 219 |
| Hsp92II | CATG | 5 cut(s) | 85, 197, 391, 466, 581 |
| Kzo9I | GATC | 1 cut(s) | 20 |
| LmnI | GCTCC | 1 cut(s) | 649 |
| LpnPI | CCDG | 5 cut(s) | 197, 530, 600, 629, 710 |
| Lsp1109I | GCAGC | 2 cut(s) | 130, 162 |
| LweI | GCATC | 2 cut(s) | 349, 434 |
| MaeI | CTAG | 1 cut(s) | 657 |
| MaeII | ACGT | 1 cut(s) | 219 |
| MaeIII | GTNAC | 1 cut(s) | 512 |
| MalI | GATC | 1 cut(s) | 22 |
| MboI | GATC | 1 cut(s) | 20 |
| MboII | GAAGA | 8 cut(s) | 59, 151, 153, 325, 392, 547, 610, 743 |
| MfeI | CAATTG | 2 cut(s) | 144, 587 |
| MhlI | GDGCHC | 1 cut(s) | 307 |
| MluCI | AATT | 9 cut(s) | 106, 144, 226, 294, 339, 353, 587, 662, 673 |
| MlyI | GAGTC | 1 cut(s) | 702 |
| MmeI | TCCRAC | 1 cut(s) | 413 |
| MnlI | CCTC | 9 cut(s) | 42, 52, 108, 111, 142, 277, 489, 640, 742 |
| Mph1103I | ATGCAT | 1 cut(s) | 273 |
| MunI | CAATTG | 2 cut(s) | 144, 587 |
| Mva1269I | GAATGC | 2 cut(s) | 485, 604 |
| MvnI | CGCG | 1 cut(s) | 282 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 677 |
| NdeII | GATC | 1 cut(s) | 20 |
| NlaIII | CATG | 5 cut(s) | 85, 197, 391, 466, 581 |
| NsiI | ATGCAT | 1 cut(s) | 273 |
| NspI | RCATGY | 3 cut(s) | 85, 391, 466 |
| NspV | TTCGAA | 1 cut(s) | 378 |
| PciI | ACATGT | 2 cut(s) | 81, 462 |
| PctI | GAATGC | 2 cut(s) | 485, 604 |
| PfeI | GAWTC | 1 cut(s) | 592 |
| PkrI | GCNGC | 2 cut(s) | 120, 177 |
| PleI | GAGTC | 1 cut(s) | 701 |
| PpsI | GAGTC | 1 cut(s) | 701 |
| PscI | ACATGT | 2 cut(s) | 81, 462 |
| RsaI | GTAC | 5 cut(s) | 218, 258, 410, 446, 622 |
| RsaNI | GTAC | 5 cut(s) | 217, 257, 409, 445, 621 |
| SatI | GCNGC | 2 cut(s) | 119, 176 |
| Sau3AI | GATC | 1 cut(s) | 20 |
| ScaI | AGTACT | 2 cut(s) | 410, 446 |
| SchI | GAGTC | 1 cut(s) | 702 |
| SduI | GDGCHC | 1 cut(s) | 307 |
| SetI | ASST | 9 cut(s) | 34, 44, 100, 123, 222, 519, 543, 654, 709 |
| SfaNI | GCATC | 2 cut(s) | 349, 434 |
| SfuI | TTCGAA | 1 cut(s) | 378 |
| SmlI | CTYRAG | 1 cut(s) | 484 |
| SmoI | CTYRAG | 1 cut(s) | 484 |
| Sse9I | AATT | 9 cut(s) | 106, 144, 226, 294, 339, 353, 587, 662, 673 |
| SspMI | CTAG | 1 cut(s) | 657 |
| TaaI | ACNGT | 2 cut(s) | 169, 553 |
| TaiI | ACGT | 1 cut(s) | 222 |
| TaqI | TCGA | 2 cut(s) | 378, 721 |
| TasI | AATT | 9 cut(s) | 106, 144, 226, 294, 339, 353, 587, 662, 673 |
| TatI | WGTACW | 4 cut(s) | 256, 408, 444, 620 |
| TfiI | GAWTC | 1 cut(s) | 592 |
| TscAI | CASTG | 2 cut(s) | 174, 558 |
| TseI | GCWGC | 2 cut(s) | 118, 175 |
| TspDTI | ATGAA | 6 cut(s) | 17, 141, 381, 492, 566, 651 |
| TspGWI | ACGGA | 2 cut(s) | 470, 647 |
| TspRI | CASTG | 2 cut(s) | 174, 558 |
| XapI | RAATTY | 1 cut(s) | 662 |
| XceI | RCATGY | 3 cut(s) | 85, 391, 466 |
| XspI | CTAG | 1 cut(s) | 657 |
| ZrmI | AGTACT | 2 cut(s) | 410, 446 |
| Zsp2I | ATGCAT | 1 cut(s) | 273 |