RchiOBHm_Chr5g0039501

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000162
Physical Location & Seq
Forward (+)
34179624 .. 34181870
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31807

Sequence Viewer

Length: 2247 bp
ATGGAGAGAAAAATTATCTTGCTTGATGAAGGATGGGGCCATATGCAGATGGGGATCACCAAGCTGAAGAGAATTCTCGAAGGACTACCGGAGCCTCAGTTCAGCTCACAGGAATACATGAATCTTTACACGGTGATTTACAACATGTGTGTTCAGAAGCCCCCTCACGACTACTCTGAAAAGCTCTATAATAATTACGGCGACGCCTTCGTCGAGTACATCGAGTCGACGGTGTTGCCTTCCCTGAGAGAGAAGCACGACGAGTTCATGATGCGTGAGCTCGTCCGAAGATGGTCCAATCACAAACTCATGGTCCGGTGGCTCACGCGCTTCTTTCAGTATCTCGACCGCTACTACGTCTGCCGAAACTCCAAGGATCCGCTCAAGGATGCTGGTCTTCAGCGATTTAAGGATCACGTTTACAACGAGATGAATGCAAAGGCTAGGGACTCTCTGATAGCTCTTATTTTGAGGGAACGTGAAGGGGAGCAGATTGACAGAGCGCTGATGAAGAACGTGGTTGATGTATTCGTGGAGGTTGGAATGGGAGAGATGGTGAATTACGAGGAGGAGTTCGAAAAACACATGGTTGATGATACTGGGGACTACTACTCTCGAAAGGCCTCGATTTGGATTGTGGAGGATTCTTGTCCCGAGTACATGATGAAGGCGGAGGAGGCCTTGAGGAAGGAGAAGGAGAGAGTTTCCCATTACCTACATTCGGGCAGCGAGCAAAAGCTGTTGGAGAGGGTGCAGCAAGAGTTGTTGGTGGTTCAAGGTCCACAGCTGCTTGATAAAGAGGGGTCTGGATGCCGGACCTTGCTTCGAGACGATAGGGTGGATGACTTGTCTAGGATGTTCAGGCTTTACCATAAAATATCCCAAGGGTTGGATCCTGTTGCTGCTATGTTTAAGAAGCATGTTACTAATGAAGGCACAGCCTTGGTCCAACAGGCCGAAGAGGCAACAGCAGCTGCAAGTGGCAACAAGCAAGGTGGTGGTGCTGGTGAGCCGGGACTAGTATTTATCAGCAAAATACTTGAGCTACATGATAAGTACATGGGATATGTCCTTAGCTGCTTCCAAAACCACTCGCTCTTCCACAAGGCCTTGAAAGAGGCTTTTGAGGTATTCTGTAATAAATCTGTTGCTGGGTACTCCAGTGCAGAATTGCTTGCTACATTCTGTGATAGTATCCTCAAGCAGATGGGTGCGAGTGATCAGGAGGCCGTAGAAGAAACTCTTGACAAGATTGTTACGGTGCTTGCATATTTCAGTGACAAGGATCTTTTCGCCGAATTCTGCAGGAAGAAACTTTCCCGTAGGCTGCTCATGACTCCTGATGATAAGAACAAGAACCGTGCTGACCAGCTTGACAATGAGAGAATGCTTCTAACAAAGCTGAAACAGCAATGTGGAGGACAGTTCACCTCTAAGATGGAGGGAATGGTCACAGATATTACTATTTCTCGGGAAAGCGAGAAGAATTTCAAAGACTATCTTGGAAAAGATGGTACTCCAAAGCTTGAGTTGGATTTCTCAGTCACTGTTCTAACAACTGGTAACTGGCCAAGCTATAAAACATCTGATCTTCACCTTCCTGAAGAGATGGTCAAGTGCATTGAAGTGTATAAAGAATATTATGACAGAGAAAAGAAACACAGGAAGCTTTCATGGATTTATTCATTGGGTACTTGCCATGTTAGGGGCAACTTTGAGCCAAAACCCATTGAAATGCATATGTCAACCTATCAGGCTGCTCTCCTGCTGCTCTTCAACACCTCGGAGAAATTGAGCTTTTCGGAGATATTGACTCAATTAAATCTTACCAAAGATGATGTCCACAGAACGCTTCATTCATTTTCTTGTGCCAAGTACAAGATCCTTCTCAAAGAGCCTAACACAAAGACAATCTCCCCCAGTGACACCTTTATGTTCAACTCTAAGTTCACCGACAGAAACAGGAAAATCAGGATCCCTCTGCCACCGCTGCAGGATGAAAGGAAGAAGGTCACGGAAGATGTCGACAAAGAGCGCAAGTATACAATTGACGCTGCACTGGTGAGGATTATGAAGAGTCGAAAAATATTGGGTCATCAACAACTGGTCACTGAGTGTCTTGAACAGTTGCAGCGCACGTTCAAGCCGGACATCAAAACAATCAAGAGGCGCATTGAAGATCTCATCACCCGTGACTACCTTGAGAGGGACCCTGAGAATCCTAACACATTTAAGTATGTCGCATGA

Protein Analysis

748

Amino Acids

87.22

Weight (kDa)

6.87

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 25 - 492 2.4e-120 Cullin alpha solenoid domain
Cullin_AB PF26557 517 - 650 1e-42 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 678 - 740 2.3e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 3 cut(s) 227, 2025, 2042
AccII CGCG 1 cut(s) 328
AciI CCGC 4 cut(s) 349, 380, 671, 1988
AcoI YGGCCR 1 cut(s) 1568
AcsI RAATTY 3 cut(s) 72, 1298, 1486
AcuI CTGAAG 3 cut(s) 86, 383, 1623
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 1 cut(s) 2115
AfaI GTAC 7 cut(s) 218, 659, 1058, 1157, 1516, 1693, 1877
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1705, 2206
AflIII ACRYGT 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 1018
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 822
AlwNI CAGNNNCTG 2 cut(s) 974, 1547
Ama87I CYCGRG 2 cut(s) 653, 1470
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 7 cut(s) 37, 621, 678, 954, 1107, 1227, 1568
ApoI RAATTY 3 cut(s) 72, 1298, 1486
ArsI GACNNNNNNTTYG 4 cut(s) 297, 329, 1824, 1856
Asp700I GAANNNNTTC 1 cut(s) 1487
AspLEI GCGC 5 cut(s) 330, 505, 2037, 2136, 2172
AspS9I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2209
AsuC2I CCSGG 1 cut(s) 1014
AsuHPI GGTGA 9 cut(s) 49, 145, 568, 1019, 1420, 1586, 1942, 2074, 2179
AsuII TTCGAA 1 cut(s) 576
AvaI CYCGRG 2 cut(s) 653, 1470
AvaII GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2209
BaeI ACNNNNGTAYC 2 cut(s) 1147, 1180
BalI TGGCCA 1 cut(s) 1570
BamHI GGATCC 3 cut(s) 376, 892, 1974
BanII GRGCYC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 282
BccI CCATC 8 cut(s) 27, 43, 285, 547, 1201, 1432, 1505, 1603
BceAI ACGGC 2 cut(s) 214, 1214
BcgI CGANNNNNNTGC 4 cut(s) 217, 251, 416, 450
BciVI GTATCC 1 cut(s) 1205
BclI TGATCA 1 cut(s) 1219
BcnI CCSGG 1 cut(s) 1014
BcoDI GTCTC 1 cut(s) 822
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 3 cut(s) 444, 852, 1019
BfmI CTRYAG 2 cut(s) 1303, 1991
BfoI RGCGCY 1 cut(s) 506
BfuI GTATCC 1 cut(s) 1205
BglI GCCNNNNNGGC 1 cut(s) 962
BglII AGATCT 1 cut(s) 2179
Bme1390I CCNGG 1 cut(s) 1014
Bme18I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2209
BmeT110I CYCGRG 2 cut(s) 653, 1470
BmgT120I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2209
BmiI GGNNCC 7 cut(s) 38, 93, 378, 894, 1976, 2210, 2211
BmrFI CCNGG 1 cut(s) 1014
BmrI ACTGGG 2 cut(s) 609, 1914
BmsI GCATC 3 cut(s) 261, 379, 800
BmuI ACTGGG 2 cut(s) 609, 1914
BpiI GAAGAC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 1144
Bpu10I CCTNAGC 1 cut(s) 1073
Bpu14I TTCGAA 1 cut(s) 576
BpuEI CTTGAG 6 cut(s) 368, 703, 1061, 1184, 1547, 2222
BpuMI CCSGG 1 cut(s) 1014
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 4 cut(s) 372, 883, 942, 1782
BsaWI WCCGGW 2 cut(s) 88, 315
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 540, 570
Bsc4I CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1705, 2206
Bse1I ACTGG 7 cut(s) 604, 1161, 1564, 1571, 1920, 2064, 2109
Bse3DI GCAATG 1 cut(s) 1418
Bse8I GATNNNNATC 1 cut(s) 53
BseDI CCNNGG 4 cut(s) 372, 883, 942, 1782
BseGI GGATG 6 cut(s) 38, 394, 815, 847, 861, 2002
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1705, 2206
BseMI GCAATG 1 cut(s) 1418
BseMII CTCAG 5 cut(s) 110, 236, 1554, 2103, 2205
BseNI ACTGG 7 cut(s) 604, 1161, 1564, 1571, 1920, 2064, 2109
BseRI GAGGAG 3 cut(s) 581, 584, 689
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 3 cut(s) 773, 1185, 2040
Bsh1236I CGCG 1 cut(s) 328
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1229, 1570
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 2 cut(s) 653, 1470
BsiSI CCGG 5 cut(s) 89, 316, 814, 1013, 2147
BslFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2222
BslI CCNNNNNNNGG 5 cut(s) 630, 721, 889, 1705, 2206
BsmAI GTCTC 1 cut(s) 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2222
BsmI GAATGC 2 cut(s) 439, 1392
BsnI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1229, 1570
BsoBI CYCGRG 2 cut(s) 653, 1470
Bsp119I TTCGAA 1 cut(s) 576
Bsp1286I GDGCHC 1 cut(s) 282
BspACI CCGC 4 cut(s) 349, 380, 671, 1988
BspANI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1229, 1570
BspCNI CTCAG 5 cut(s) 109, 237, 1553, 2104, 2206
BspFNI CGCG 1 cut(s) 328
BspHI TCATGA 2 cut(s) 267, 1332
BspLI GGNNCC 7 cut(s) 38, 93, 378, 894, 1976, 2210, 2211
BspMAI CTGCAG 2 cut(s) 1307, 1995
BspQI GCTCTTC 2 cut(s) 1103, 1778
BspT104I TTCGAA 1 cut(s) 576
BsrBI CCGCTC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 1418
BsrI ACTGG 7 cut(s) 604, 1161, 1564, 1571, 1920, 2064, 2109
BssECI CCNNGG 4 cut(s) 372, 883, 942, 1782
BssNAI GTATAC 1 cut(s) 2043
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 3 cut(s) 372, 883, 942
Bst1107I GTATAC 1 cut(s) 2043
Bst4CI ACNGT 7 cut(s) 133, 232, 1261, 1361, 1425, 1549, 2127
Bst6I CTCTTC 6 cut(s) 62, 954, 1103, 1599, 1778, 2069
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 731, 1176, 1266
BstDEI CTNAG 8 cut(s) 96, 245, 1073, 1434, 1540, 1944, 2112, 2214
BstF5I GGATG 6 cut(s) 38, 394, 815, 847, 861, 2002
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 5 cut(s) 330, 505, 2037, 2136, 2172
BstMAI GTCTC 1 cut(s) 822
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1408, 1990
BstNSI RCATGY 2 cut(s) 148, 923
BstSCI CCNGG 1 cut(s) 1012
BstSFI CTRYAG 2 cut(s) 1303, 1991
BstUI CGCG 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 6 cut(s) 376, 892, 1285, 1881, 1974, 2179
BstYI RGATCY 6 cut(s) 376, 892, 1285, 1881, 1974, 2179
BstZ17I GTATAC 1 cut(s) 2043
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1229, 1570
BtsCI GGATG 6 cut(s) 38, 394, 815, 847, 861, 2002
BtsIMutI CAGTG 6 cut(s) 1168, 1282, 1545, 1927, 2057, 2109
Cac8I GCNNGC 3 cut(s) 731, 1176, 1266
CaiI CAGNNNCTG 2 cut(s) 974, 1547
CciI TCATGA 2 cut(s) 267, 1332
CfoI GCGC 5 cut(s) 330, 505, 2037, 2136, 2172
Cfr13I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2209
CseI GACGC 2 cut(s) 212, 2060
Csp6I GTAC 7 cut(s) 217, 658, 1057, 1156, 1515, 1692, 1876
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 7 cut(s) 217, 658, 1057, 1156, 1515, 1692, 1876
DdeI CTNAG 8 cut(s) 96, 245, 1073, 1434, 1540, 1944, 2112, 2214
DraIII CACNNNGTG 1 cut(s) 2115
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 1568
Eam1104I CTCTTC 6 cut(s) 62, 954, 1103, 1599, 1778, 2069
EarI CTCTTC 6 cut(s) 62, 954, 1103, 1599, 1778, 2069
EciI GGCGGA 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 372, 883, 942
Eco147I AGGCCT 3 cut(s) 623, 680, 1109
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2209
Eco47III AGCGCT 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 3 cut(s) 86, 383, 1623
Eco88I CYCGRG 2 cut(s) 653, 1470
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 2209
EcoRI GAATTC 2 cut(s) 72, 1298
EcoT14I CCWWGG 3 cut(s) 372, 883, 942
EcoT22I ATGCAT 1 cut(s) 1740
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 3 cut(s) 372, 883, 942
Esp3I CGTCTC 1 cut(s) 822
FalI AAGNNNNNCTT 4 cut(s) 1227, 1259, 1485, 1517
FaqI GGGAC 5 cut(s) 461, 617, 636, 1029, 2222
FauNDI CATATG 2 cut(s) 42, 1740
FbaI TGATCA 1 cut(s) 1219
FblI GTMKAC 3 cut(s) 227, 2025, 2042
FokI GGATG 6 cut(s) 45, 401, 822, 854, 868, 2009
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 3 cut(s) 444, 852, 1019
GlaI GCGC 5 cut(s) 329, 504, 2036, 2135, 2171
GsaI CCCAGC 1 cut(s) 1155
GsuI CTGGAG 1 cut(s) 1144
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1229, 1570
HapII CCGG 5 cut(s) 89, 316, 814, 1013, 2147
HgaI GACGC 2 cut(s) 212, 2060
HhaI GCGC 5 cut(s) 330, 505, 2037, 2136, 2172
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 5 cut(s) 328, 503, 2035, 2134, 2170
HinP1I GCGC 5 cut(s) 328, 503, 2035, 2134, 2170
HincII GTYRAC 3 cut(s) 228, 1746, 2026
HindII GTYRAC 3 cut(s) 228, 1746, 2026
HindIII AAGCTT 2 cut(s) 1523, 1667
HinfI GANTC 8 cut(s) 121, 224, 449, 644, 1336, 1813, 2077, 2218
HpaII CCGG 5 cut(s) 89, 316, 814, 1013, 2147
HphI GGTGA 9 cut(s) 49, 145, 568, 1019, 1420, 1586, 1942, 2074, 2179
Hpy166II GTNNAC 9 cut(s) 228, 421, 782, 1428, 1746, 1843, 1950, 2026, 2043
Hpy188I TCNGA 7 cut(s) 156, 178, 287, 456, 1588, 1786, 1804
Hpy8I GTNNAC 9 cut(s) 228, 421, 782, 1428, 1746, 1843, 1950, 2026, 2043
Hpy99I CGWCG 4 cut(s) 206, 215, 232, 263
HpyCH4III ACNGT 7 cut(s) 133, 232, 1261, 1361, 1425, 1549, 2127
HpyCH4IV ACGT 5 cut(s) 357, 417, 478, 516, 2138
HpyF10VI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1408, 1990
HpyF3I CTNAG 8 cut(s) 96, 245, 1073, 1434, 1540, 1944, 2112, 2214
HpySE526I ACGT 5 cut(s) 357, 417, 478, 516, 2138
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 5 cut(s) 328, 503, 2035, 2134, 2170
KflI GGGWCCC 1 cut(s) 2209
Ksp22I TGATCA 1 cut(s) 1219
LguI GCTCTTC 2 cut(s) 1103, 1778
LmnI GCTCC 2 cut(s) 91, 487
LweI GCATC 3 cut(s) 261, 379, 800
MaeI CTAG 3 cut(s) 444, 852, 1019
MaeII ACGT 5 cut(s) 357, 417, 478, 516, 2138
MbiI CCGCTC 1 cut(s) 382
MfeI CAATTG 1 cut(s) 2046
MflI RGATCY 6 cut(s) 376, 892, 1285, 1881, 1974, 2179
MhlI GDGCHC 1 cut(s) 282
MlsI TGGCCA 1 cut(s) 1570
MluNI TGGCCA 1 cut(s) 1570
MlyI GAGTC 5 cut(s) 233, 443, 1330, 1807, 2086
MmeI TCCRAC 5 cut(s) 520, 723, 870, 973, 1512
Mox20I TGGCCA 1 cut(s) 1570
Mph1103I ATGCAT 1 cut(s) 1740
MroXI GAANNNNTTC 1 cut(s) 1487
MscI TGGCCA 1 cut(s) 1570
MseI TTAA 4 cut(s) 408, 912, 1820, 2232
MslI CAYNNNNRTG 3 cut(s) 1625, 1733, 1931
Msp20I TGGCCA 1 cut(s) 1570
MspA1I CMGCKG 3 cut(s) 787, 974, 1990
MspI CCGG 5 cut(s) 89, 316, 814, 1013, 2147
MspR9I CCNGG 1 cut(s) 1014
MunI CAATTG 1 cut(s) 2046
Mva1269I GAATGC 2 cut(s) 439, 1392
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1408, 1990
NciI CCSGG 1 cut(s) 1014
NdeI CATATG 2 cut(s) 42, 1740
NlaIV GGNNCC 7 cut(s) 38, 93, 378, 894, 1976, 2210, 2211
NmuCI GTSAC 7 cut(s) 1277, 1450, 1543, 1922, 2011, 2107, 2192
NsiI ATGCAT 1 cut(s) 1740
NspI RCATGY 2 cut(s) 148, 923
NspV TTCGAA 1 cut(s) 576
PagI TCATGA 2 cut(s) 267, 1332
PceI AGGCCT 3 cut(s) 623, 680, 1109
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 2 cut(s) 1103, 1778
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 219, 423
PctI GAATGC 2 cut(s) 439, 1392
PdmI GAANNNNTTC 1 cut(s) 1487
PfeI GAWTC 3 cut(s) 121, 644, 2218
PflMI CCANNNNNTGG 1 cut(s) 889
PleI GAGTC 5 cut(s) 232, 443, 1330, 1807, 2085
PpsI GAGTC 5 cut(s) 232, 443, 1330, 1807, 2085
PpuMI RGGWCCY 1 cut(s) 2209
PscI ACATGT 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 2209
PspFI CCCAGC 1 cut(s) 1151
PspN4I GGNNCC 7 cut(s) 38, 93, 378, 894, 1976, 2210, 2211
PspPI GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2209
PspPPI RGGWCCY 1 cut(s) 2209
PstI CTGCAG 2 cut(s) 1307, 1995
PstNI CAGNNNCTG 2 cut(s) 974, 1547
PsuI RGATCY 6 cut(s) 376, 892, 1285, 1881, 1974, 2179
PvuII CAGCTG 2 cut(s) 787, 974
RsaI GTAC 7 cut(s) 218, 659, 1058, 1157, 1516, 1693, 1877
RsaNI GTAC 7 cut(s) 217, 658, 1057, 1156, 1515, 1692, 1876
RseI CAYNNNNRTG 3 cut(s) 1625, 1733, 1931
SacI GAGCTC 1 cut(s) 282
SalI GTCGAC 2 cut(s) 226, 2024
SapI GCTCTTC 2 cut(s) 1103, 1778
SaqAI TTAA 4 cut(s) 408, 912, 1820, 2232
Sau96I GGNCC 7 cut(s) 37, 294, 313, 779, 816, 946, 2209
SchI GAGTC 5 cut(s) 233, 443, 1330, 1807, 2086
ScrFI CCNGG 1 cut(s) 1014
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 3 cut(s) 261, 379, 800
SfcI CTRYAG 2 cut(s) 1303, 1991
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2209
SmiMI CAYNNNNRTG 3 cut(s) 1625, 1733, 1931
SmlI CTYRAG 6 cut(s) 383, 682, 1040, 1199, 1526, 2201
SmoI CTYRAG 6 cut(s) 383, 682, 1040, 1199, 1526, 2201
SpeI ACTAGT 1 cut(s) 1018
SseBI AGGCCT 3 cut(s) 623, 680, 1109
SsiI CCGC 4 cut(s) 349, 380, 671, 1988
SspI AATATT 2 cut(s) 1640, 2088
SspMI CTAG 3 cut(s) 444, 852, 1019
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 3 cut(s) 623, 680, 1109
StyD4I CCNGG 1 cut(s) 1012
StyI CCWWGG 3 cut(s) 372, 883, 942
TaaI ACNGT 7 cut(s) 133, 232, 1261, 1361, 1425, 1549, 2127
TaiI ACGT 5 cut(s) 360, 420, 481, 519, 2141
TatI WGTACW 4 cut(s) 216, 657, 1056, 1875
TfiI GAWTC 3 cut(s) 121, 644, 2218
Tru1I TTAA 4 cut(s) 408, 912, 1820, 2232
Tru9I TTAA 4 cut(s) 408, 912, 1820, 2232
TscAI CASTG 6 cut(s) 1168, 1282, 1552, 1927, 2064, 2116
TseFI GTSAC 7 cut(s) 1277, 1450, 1543, 1922, 2011, 2107, 2192
Tsp45I GTSAC 7 cut(s) 1277, 1450, 1543, 1922, 2011, 2107, 2192
TspGWI ACGGA 1 cut(s) 2030
TspRI CASTG 6 cut(s) 1168, 1282, 1552, 1927, 2064, 2116
Van91I CCANNNNNTGG 1 cut(s) 889
VpaK11BI GGWCC 6 cut(s) 294, 313, 779, 816, 946, 2209
XapI RAATTY 3 cut(s) 72, 1298, 1486
XceI RCATGY 2 cut(s) 148, 923
XmiI GTMKAC 3 cut(s) 227, 2025, 2042
XmnI GAANNNNTTC 1 cut(s) 1487
XspI CTAG 3 cut(s) 444, 852, 1019
Zsp2I ATGCAT 1 cut(s) 1740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.