Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1517 |
| Acc36I | ACCTGC | 1 cut(s) | 967 |
| AccBSI | CCGCTC | 2 cut(s) | 475, 2011 |
| AccII | CGCG | 1 cut(s) | 613 |
| AciI | CCGC | 7 cut(s) | 271, 475, 578, 590, 620, 1961, 2011 |
| AclWI | GGATC | 3 cut(s) | 62, 1514, 1852 |
| AcoI | YGGCCR | 1 cut(s) | 1544 |
| AcsI | RAATTY | 3 cut(s) | 157, 1026, 1295 |
| AcuI | CTGAAG | 2 cut(s) | 86, 2013 |
| AfaI | GTAC | 4 cut(s) | 116, 215, 710, 866 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1867, 1966, 2182 |
| AflIII | ACRYGT | 2 cut(s) | 144, 916 |
| AhlI | ACTAGT | 1 cut(s) | 1202 |
| AjnI | CCWGG | 3 cut(s) | 939, 1501, 2175 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1461, 1493 |
| AleI | CACNNNNGTG | 1 cut(s) | 1840 |
| Alw21I | GWGCWC | 2 cut(s) | 88, 1038 |
| AlwI | GGATC | 3 cut(s) | 62, 1514, 1852 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 736, 1523 |
| AoxI | GGCC | 4 cut(s) | 291, 936, 951, 1544 |
| ApeKI | GCWGC | 9 cut(s) | 232, 268, 751, 962, 1258, 1324, 1733, 1744, 2030 |
| ApoI | RAATTY | 3 cut(s) | 157, 1026, 1295 |
| AspLEI | GCGC | 2 cut(s) | 615, 2148 |
| AspS9I | GGNCC | 4 cut(s) | 291, 937, 1012, 2185 |
| AsuC2I | CCSGG | 3 cut(s) | 90, 391, 650 |
| AsuHPI | GGTGA | 5 cut(s) | 269, 634, 673, 839, 1999 |
| AsuII | TTCGAA | 1 cut(s) | 1467 |
| AvaII | GGWCC | 2 cut(s) | 1012, 2185 |
| BalI | TGGCCA | 1 cut(s) | 1546 |
| BanII | GRGCYC | 2 cut(s) | 88, 1038 |
| BbsI | GAAGAC | 3 cut(s) | 1356, 1889, 2199 |
| Bbv12I | GWGCWC | 2 cut(s) | 88, 1038 |
| BbvI | GCAGC | 9 cut(s) | 219, 255, 763, 949, 1245, 1311, 1720, 1731, 2017 |
| BccI | CCATC | 6 cut(s) | 27, 143, 248, 282, 535, 2095 |
| BceAI | ACGGC | 1 cut(s) | 211 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 214, 248, 944, 978 |
| BciT130I | CCWGG | 3 cut(s) | 941, 1503, 2177 |
| BclI | TGATCA | 1 cut(s) | 1587 |
| BcnI | CCSGG | 3 cut(s) | 90, 391, 650 |
| BcuI | ACTAGT | 1 cut(s) | 1202 |
| BfaI | CTAG | 6 cut(s) | 77, 375, 849, 900, 1203, 1679 |
| BfmI | CTRYAG | 1 cut(s) | 1300 |
| BfuAI | ACCTGC | 1 cut(s) | 967 |
| BglII | AGATCT | 1 cut(s) | 2155 |
| BisI | GCNGC | 9 cut(s) | 233, 269, 752, 963, 1259, 1325, 1734, 1745, 2031 |
| BlsI | GCNGC | 9 cut(s) | 234, 270, 753, 964, 1260, 1326, 1735, 1746, 2032 |
| BmcAI | AGTACT | 1 cut(s) | 710 |
| Bme1390I | CCNGG | 6 cut(s) | 90, 391, 650, 941, 1503, 2177 |
| Bme18I | GGWCC | 2 cut(s) | 1012, 2185 |
| BmgT120I | GGNCC | 4 cut(s) | 291, 937, 1012, 2185 |
| BmiI | GGNNCC | 5 cut(s) | 7, 891, 1483, 1872, 2186 |
| BmrFI | CCNGG | 6 cut(s) | 90, 391, 650, 941, 1503, 2177 |
| BmsI | GCATC | 5 cut(s) | 573, 949, 1404, 2017, 2121 |
| BpiI | GAAGAC | 3 cut(s) | 1356, 1889, 2199 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 688, 720 |
| BpmI | CTGGAG | 1 cut(s) | 2198 |
| Bpu14I | TTCGAA | 1 cut(s) | 1467 |
| BpuEI | CTTGAG | 3 cut(s) | 700, 1175, 1262 |
| BpuMI | CCSGG | 3 cut(s) | 90, 391, 650 |
| Bsa29I | ATCGAT | 1 cut(s) | 789 |
| BsaJI | CCNNGG | 3 cut(s) | 253, 939, 1502 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 27, 830, 860 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1867, 1966, 2182 |
| Bse1I | ACTGG | 5 cut(s) | 1014, 1069, 1170, 1446, 1540 |
| Bse3DI | GCAATG | 1 cut(s) | 1394 |
| BseBI | CCWGG | 3 cut(s) | 941, 1503, 2177 |
| BseCI | ATCGAT | 1 cut(s) | 789 |
| BseDI | CCNNGG | 3 cut(s) | 253, 939, 1502 |
| BseGI | GGATG | 5 cut(s) | 15, 38, 526, 546, 858 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1867, 1966, 2182 |
| BseMI | GCAATG | 1 cut(s) | 1394 |
| BseMII | CTCAG | 3 cut(s) | 188, 719, 1805 |
| BseNI | ACTGG | 5 cut(s) | 1014, 1069, 1170, 1446, 1540 |
| BseRI | GAGGAG | 2 cut(s) | 125, 697 |
| BseXI | GCAGC | 9 cut(s) | 219, 255, 763, 949, 1245, 1311, 1720, 1731, 2017 |
| BseYI | CCCAGC | 2 cut(s) | 992, 1142 |
| BsgI | GTGCAG | 4 cut(s) | 169, 770, 2016, 2126 |
| Bsh1236I | CGCG | 1 cut(s) | 613 |
| BshFI | GGCC | 4 cut(s) | 293, 938, 953, 1546 |
| BshVI | ATCGAT | 1 cut(s) | 789 |
| BsiHKAI | GWGCWC | 2 cut(s) | 88, 1038 |
| BsiSI | CCGG | 3 cut(s) | 89, 390, 650 |
| BslFI | GGGAC | 3 cut(s) | 1020, 1213, 2198 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1867, 1966, 2182 |
| BsmFI | GGGAC | 3 cut(s) | 1020, 1213, 2198 |
| BsmI | GAATGC | 3 cut(s) | 680, 715, 799 |
| BsnI | GGCC | 4 cut(s) | 293, 938, 953, 1546 |
| Bsp119I | TTCGAA | 1 cut(s) | 1467 |
| Bsp1286I | GDGCHC | 2 cut(s) | 88, 1038 |
| BspACI | CCGC | 7 cut(s) | 271, 475, 578, 590, 620, 1961, 2011 |
| BspANI | GGCC | 4 cut(s) | 293, 938, 953, 1546 |
| BspCNI | CTCAG | 3 cut(s) | 187, 718, 1804 |
| BspDI | ATCGAT | 1 cut(s) | 789 |
| BspFNI | CGCG | 1 cut(s) | 613 |
| BspLI | GGNNCC | 5 cut(s) | 7, 891, 1483, 1872, 2186 |
| BspMI | ACCTGC | 1 cut(s) | 967 |
| BspPI | GGATC | 3 cut(s) | 62, 1514, 1852 |
| BspT104I | TTCGAA | 1 cut(s) | 1467 |
| BsrBI | CCGCTC | 2 cut(s) | 475, 2011 |
| BsrDI | GCAATG | 1 cut(s) | 1394 |
| BsrI | ACTGG | 5 cut(s) | 1014, 1069, 1170, 1446, 1540 |
| BssECI | CCNNGG | 3 cut(s) | 253, 939, 1502 |
| Bst2UI | CCWGG | 3 cut(s) | 941, 1503, 2177 |
| Bst4CI | ACNGT | 6 cut(s) | 229, 257, 1401, 1525, 1839, 1888 |
| Bst6I | CTCTTC | 1 cut(s) | 2045 |
| BstBI | TTCGAA | 1 cut(s) | 1467 |
| BstC8I | GCNNGC | 6 cut(s) | 588, 951, 974, 1263, 1314, 1544 |
| BstDEI | CTNAG | 4 cut(s) | 174, 705, 1791, 1829 |
| BstDSI | CCRYGG | 1 cut(s) | 253 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1865 |
| BstF5I | GGATG | 5 cut(s) | 15, 38, 526, 546, 858 |
| BstFNI | CGCG | 1 cut(s) | 613 |
| BstHHI | GCGC | 2 cut(s) | 615, 2148 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 610, 757, 959, 1384, 2027 |
| BstNI | CCWGG | 3 cut(s) | 941, 1503, 2177 |
| BstNSI | RCATGY | 1 cut(s) | 148 |
| BstSCI | CCNGG | 6 cut(s) | 88, 389, 648, 939, 1501, 2175 |
| BstSFI | CTRYAG | 1 cut(s) | 1300 |
| BstUI | CGCG | 1 cut(s) | 613 |
| BstV1I | GCAGC | 9 cut(s) | 219, 255, 763, 949, 1245, 1311, 1720, 1731, 2017 |
| BstV2I | GAAGAC | 3 cut(s) | 1356, 1889, 2199 |
| BstX2I | RGATCY | 2 cut(s) | 1857, 2155 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1711 |
| BstYI | RGATCY | 2 cut(s) | 1857, 2155 |
| Bsu15I | ATCGAT | 1 cut(s) | 789 |
| BsuRI | GGCC | 4 cut(s) | 293, 938, 953, 1546 |
| BsuTUI | ATCGAT | 1 cut(s) | 789 |
| BtgI | CCRYGG | 1 cut(s) | 253 |
| BtgZI | GCGATG | 1 cut(s) | 226 |
| BtsCI | GGATG | 5 cut(s) | 15, 38, 526, 546, 858 |
| BtsI | GCAGTG | 1 cut(s) | 730 |
| BtsIMutI | CAGTG | 5 cut(s) | 730, 1062, 1439, 1521, 1835 |
| BveI | ACCTGC | 1 cut(s) | 967 |
| Cac8I | GCNNGC | 6 cut(s) | 588, 951, 974, 1263, 1314, 1544 |
| CaiI | CAGNNNCTG | 2 cut(s) | 736, 1523 |
| CfoI | GCGC | 2 cut(s) | 615, 2148 |
| Cfr13I | GGNCC | 4 cut(s) | 291, 937, 1012, 2185 |
| ClaI | ATCGAT | 1 cut(s) | 789 |
| Csp6I | GTAC | 4 cut(s) | 115, 214, 709, 865 |
| CviQI | GTAC | 4 cut(s) | 115, 214, 709, 865 |
| DdeI | CTNAG | 4 cut(s) | 174, 705, 1791, 1829 |
| DraI | TTTAAA | 1 cut(s) | 910 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1517 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1517 |
| EaeI | YGGCCR | 1 cut(s) | 1544 |
| Eam1104I | CTCTTC | 1 cut(s) | 2045 |
| EarI | CTCTTC | 1 cut(s) | 2045 |
| EciI | GGCGGA | 1 cut(s) | 593 |
| Ecl136II | GAGCTC | 2 cut(s) | 86, 1036 |
| Eco24I | GRGCYC | 2 cut(s) | 88, 1038 |
| Eco47I | GGWCC | 2 cut(s) | 1012, 2185 |
| Eco53kI | GAGCTC | 2 cut(s) | 86, 1036 |
| Eco57I | CTGAAG | 2 cut(s) | 86, 2013 |
| EcoICRI | GAGCTC | 2 cut(s) | 86, 1036 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1865 |
| EcoO109I | RGGNCCY | 1 cut(s) | 937 |
| EcoRI | GAATTC | 1 cut(s) | 1295 |
| EcoRII | CCWGG | 3 cut(s) | 939, 1501, 2175 |
| EcoT38I | GRGCYC | 2 cut(s) | 88, 1038 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 378, 410 |
| FaqI | GGGAC | 3 cut(s) | 1020, 1213, 2198 |
| FauI | CCCGC | 3 cut(s) | 264, 468, 1968 |
| FbaI | TGATCA | 1 cut(s) | 1587 |
| Fnu4HI | GCNGC | 9 cut(s) | 233, 269, 752, 963, 1259, 1325, 1734, 1745, 2031 |
| FokI | GGATG | 5 cut(s) | 2, 45, 533, 553, 865 |
| FriOI | GRGCYC | 2 cut(s) | 88, 1038 |
| Fsp4HI | GCNGC | 9 cut(s) | 233, 269, 752, 963, 1259, 1325, 1734, 1745, 2031 |
| FspBI | CTAG | 6 cut(s) | 77, 375, 849, 900, 1203, 1679 |
| GlaI | GCGC | 2 cut(s) | 614, 2147 |
| GluI | GCNGC | 9 cut(s) | 233, 269, 752, 963, 1259, 1325, 1734, 1745, 2031 |
| GsaI | CCCAGC | 2 cut(s) | 996, 1146 |
| GsuI | CTGGAG | 1 cut(s) | 2198 |
| HaeIII | GGCC | 4 cut(s) | 293, 938, 953, 1546 |
| HapII | CCGG | 3 cut(s) | 89, 390, 650 |
| HhaI | GCGC | 2 cut(s) | 615, 2148 |
| Hin6I | GCGC | 2 cut(s) | 613, 2146 |
| HinP1I | GCGC | 2 cut(s) | 613, 2146 |
| HincII | GTYRAC | 2 cut(s) | 207, 2002 |
| HindII | GTYRAC | 2 cut(s) | 207, 2002 |
| HindIII | AAGCTT | 3 cut(s) | 1310, 1771, 1799 |
| HpaII | CCGG | 3 cut(s) | 89, 390, 650 |
| HphI | GGTGA | 5 cut(s) | 269, 634, 673, 839, 1999 |
| Hpy166II | GTNNAC | 6 cut(s) | 207, 568, 1397, 1835, 1926, 2002 |
| Hpy188I | TCNGA | 8 cut(s) | 109, 221, 489, 1338, 1458, 1564, 1762, 1780 |
| Hpy188III | TCNNGA | 9 cut(s) | 58, 77, 280, 341, 407, 804, 1021, 1192, 1241 |
| Hpy8I | GTNNAC | 6 cut(s) | 207, 568, 1397, 1835, 1926, 2002 |
| Hpy99I | CGWCG | 2 cut(s) | 229, 1323 |
| HpyCH4III | ACNGT | 6 cut(s) | 229, 257, 1401, 1525, 1839, 1888 |
| HpyCH4IV | ACGT | 1 cut(s) | 918 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 610, 757, 959, 1384, 2027 |
| HpyF3I | CTNAG | 4 cut(s) | 174, 705, 1791, 1829 |
| HpySE526I | ACGT | 1 cut(s) | 918 |
| HspAI | GCGC | 2 cut(s) | 613, 2146 |
| Ksp22I | TGATCA | 1 cut(s) | 1587 |
| Lsp1109I | GCAGC | 9 cut(s) | 219, 255, 763, 949, 1245, 1311, 1720, 1731, 2017 |
| LweI | GCATC | 5 cut(s) | 573, 949, 1404, 2017, 2121 |
| MaeI | CTAG | 6 cut(s) | 77, 375, 849, 900, 1203, 1679 |
| MaeII | ACGT | 1 cut(s) | 918 |
| MaeIII | GTNAC | 7 cut(s) | 919, 1060, 1274, 1426, 1519, 1550, 1987 |
| MbiI | CCGCTC | 2 cut(s) | 475, 2011 |
| MflI | RGATCY | 2 cut(s) | 1857, 2155 |
| MhlI | GDGCHC | 2 cut(s) | 88, 1038 |
| MlsI | TGGCCA | 1 cut(s) | 1546 |
| MluNI | TGGCCA | 1 cut(s) | 1546 |
| MlyI | GAGTC | 3 cut(s) | 209, 1783, 2189 |
| MmeI | TCCRAC | 1 cut(s) | 608 |
| Mox20I | TGGCCA | 1 cut(s) | 1546 |
| MscI | TGGCCA | 1 cut(s) | 1546 |
| MseI | TTAA | 8 cut(s) | 909, 1497, 1530, 1569, 1769, 1866, 1890, 2139 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1433, 1840 |
| Msp20I | TGGCCA | 1 cut(s) | 1546 |
| MspI | CCGG | 3 cut(s) | 89, 390, 650 |
| MspR9I | CCNGG | 6 cut(s) | 90, 391, 650, 941, 1503, 2177 |
| MssI | GTTTAAAC | 1 cut(s) | 910 |
| Mva1269I | GAATGC | 3 cut(s) | 680, 715, 799 |
| MvaI | CCWGG | 3 cut(s) | 941, 1503, 2177 |
| MvnI | CGCG | 1 cut(s) | 613 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 610, 757, 959, 1384, 2027 |
| NciI | CCSGG | 3 cut(s) | 90, 391, 650 |
| NlaIV | GGNNCC | 5 cut(s) | 7, 891, 1483, 1872, 2186 |
| NmeAIII | GCCGAG | 1 cut(s) | 271 |
| NmuCI | GTSAC | 5 cut(s) | 1060, 1274, 1426, 1519, 1987 |
| NspI | RCATGY | 1 cut(s) | 148 |
| NspV | TTCGAA | 1 cut(s) | 1467 |
| OliI | CACNNNNGTG | 1 cut(s) | 1840 |
| PciI | ACATGT | 1 cut(s) | 144 |
| PctI | GAATGC | 3 cut(s) | 680, 715, 799 |
| PfeI | GAWTC | 8 cut(s) | 73, 641, 820, 1173, 1614, 1622, 1757, 1951 |
| PkrI | GCNGC | 9 cut(s) | 234, 270, 753, 964, 1260, 1326, 1735, 1746, 2032 |
| PleI | GAGTC | 3 cut(s) | 208, 1783, 2189 |
| PmeI | GTTTAAAC | 1 cut(s) | 910 |
| PpsI | GAGTC | 3 cut(s) | 208, 1783, 2189 |
| PscI | ACATGT | 1 cut(s) | 144 |
| Psp124BI | GAGCTC | 2 cut(s) | 88, 1038 |
| Psp6I | CCWGG | 3 cut(s) | 939, 1501, 2175 |
| PspFI | CCCAGC | 2 cut(s) | 992, 1142 |
| PspGI | CCWGG | 3 cut(s) | 939, 1501, 2175 |
| PspN4I | GGNNCC | 5 cut(s) | 7, 891, 1483, 1872, 2186 |
| PspPI | GGNCC | 4 cut(s) | 291, 937, 1012, 2185 |
| PstNI | CAGNNNCTG | 2 cut(s) | 736, 1523 |
| PsuI | RGATCY | 2 cut(s) | 1857, 2155 |
| RsaI | GTAC | 4 cut(s) | 116, 215, 710, 866 |
| RsaNI | GTAC | 4 cut(s) | 115, 214, 709, 865 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1433, 1840 |
| SacI | GAGCTC | 2 cut(s) | 88, 1038 |
| SaqAI | TTAA | 8 cut(s) | 909, 1497, 1530, 1569, 1769, 1866, 1890, 2139 |
| SatI | GCNGC | 9 cut(s) | 233, 269, 752, 963, 1259, 1325, 1734, 1745, 2031 |
| Sau96I | GGNCC | 4 cut(s) | 291, 937, 1012, 2185 |
| ScaI | AGTACT | 1 cut(s) | 710 |
| SchI | GAGTC | 3 cut(s) | 209, 1783, 2189 |
| ScrFI | CCNGG | 6 cut(s) | 90, 391, 650, 941, 1503, 2177 |
| SduI | GDGCHC | 2 cut(s) | 88, 1038 |
| SfaNI | GCATC | 5 cut(s) | 573, 949, 1404, 2017, 2121 |
| SfcI | CTRYAG | 1 cut(s) | 1300 |
| SfuI | TTCGAA | 1 cut(s) | 1467 |
| SinI | GGWCC | 2 cut(s) | 1012, 2185 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1433, 1840 |
| SmlI | CTYRAG | 3 cut(s) | 679, 1190, 1241 |
| SmoI | CTYRAG | 3 cut(s) | 679, 1190, 1241 |
| SpeI | ACTAGT | 1 cut(s) | 1202 |
| SsiI | CCGC | 7 cut(s) | 271, 475, 578, 590, 620, 1961, 2011 |
| SspI | AATATT | 1 cut(s) | 1785 |
| SspMI | CTAG | 6 cut(s) | 77, 375, 849, 900, 1203, 1679 |
| SstI | GAGCTC | 2 cut(s) | 88, 1038 |
| StyD4I | CCNGG | 6 cut(s) | 88, 389, 648, 939, 1501, 2175 |
| TaaI | ACNGT | 6 cut(s) | 229, 257, 1401, 1525, 1839, 1888 |
| TaiI | ACGT | 1 cut(s) | 921 |
| TaqI | TCGA | 7 cut(s) | 24, 719, 789, 1151, 1158, 1467, 1509 |
| TaqII | GACCGA | 1 cut(s) | 1075 |
| TatI | WGTACW | 3 cut(s) | 114, 213, 708 |
| TfiI | GAWTC | 8 cut(s) | 73, 641, 820, 1173, 1614, 1622, 1757, 1951 |
| Tru1I | TTAA | 8 cut(s) | 909, 1497, 1530, 1569, 1769, 1866, 1890, 2139 |
| Tru9I | TTAA | 8 cut(s) | 909, 1497, 1530, 1569, 1769, 1866, 1890, 2139 |
| TscAI | CASTG | 5 cut(s) | 730, 1069, 1446, 1528, 1842 |
| TseFI | GTSAC | 5 cut(s) | 1060, 1274, 1426, 1519, 1987 |
| TseI | GCWGC | 9 cut(s) | 232, 268, 751, 962, 1258, 1324, 1733, 1744, 2030 |
| Tsp45I | GTSAC | 5 cut(s) | 1060, 1274, 1426, 1519, 1987 |
| TspDTI | ATGAA | 7 cut(s) | 576, 761, 942, 1091, 1989, 2063, 2205 |
| TspGWI | ACGGA | 2 cut(s) | 224, 1002 |
| TspRI | CASTG | 5 cut(s) | 730, 1069, 1446, 1528, 1842 |
| VpaK11BI | GGWCC | 2 cut(s) | 1012, 2185 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1865 |
| XapI | RAATTY | 3 cut(s) | 157, 1026, 1295 |
| XbaI | TCTAGA | 1 cut(s) | 76 |
| XceI | RCATGY | 1 cut(s) | 148 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1813 |
| XspI | CTAG | 6 cut(s) | 77, 375, 849, 900, 1203, 1679 |
| ZrmI | AGTACT | 1 cut(s) | 710 |