Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 209 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 889 |
| AccBSI | CCGCTC | 1 cut(s) | 382 |
| AccI | GTMKAC | 3 cut(s) | 227, 2022, 2039 |
| AccII | CGCG | 1 cut(s) | 328 |
| AciI | CCGC | 4 cut(s) | 349, 380, 671, 1985 |
| AcoI | YGGCCR | 1 cut(s) | 1565 |
| AcsI | RAATTY | 2 cut(s) | 72, 1298 |
| AcuI | CTGAAG | 2 cut(s) | 86, 1620 |
| AcyI | GRCGYC | 1 cut(s) | 204 |
| AdeI | CACNNNGTG | 1 cut(s) | 2112 |
| AfaI | GTAC | 6 cut(s) | 218, 1058, 1157, 1513, 1690, 1874 |
| AfeI | AGCGCT | 1 cut(s) | 504 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 630, 721, 889, 1702, 2203 |
| AflII | CTTAAG | 1 cut(s) | 398 |
| AflIII | ACRYGT | 1 cut(s) | 144 |
| AhlI | ACTAGT | 1 cut(s) | 1018 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 83, 115 |
| Alw21I | GWGCWC | 1 cut(s) | 282 |
| Alw26I | GTCTC | 1 cut(s) | 822 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 974 |
| Ama87I | CYCGRG | 2 cut(s) | 653, 1159 |
| Aor51HI | AGCGCT | 1 cut(s) | 504 |
| AoxI | GGCC | 7 cut(s) | 37, 621, 678, 954, 1107, 1227, 1565 |
| ApoI | RAATTY | 2 cut(s) | 72, 1298 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 297, 329, 1821, 1853 |
| AseI | ATTAAT | 1 cut(s) | 1335 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1484 |
| AspLEI | GCGC | 5 cut(s) | 330, 505, 2034, 2133, 2169 |
| AspS9I | GGNCC | 7 cut(s) | 37, 294, 313, 779, 816, 946, 2206 |
| AsuC2I | CCSGG | 1 cut(s) | 1014 |
| AsuHPI | GGTGA | 8 cut(s) | 49, 568, 1019, 1417, 1583, 1939, 2071, 2176 |
| AsuII | TTCGAA | 2 cut(s) | 576, 1501 |
| AvaI | CYCGRG | 2 cut(s) | 653, 1159 |
| AvaII | GGWCC | 6 cut(s) | 294, 313, 779, 816, 946, 2206 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1147, 1180 |
| BalI | TGGCCA | 1 cut(s) | 1567 |
| BamHI | GGATCC | 3 cut(s) | 376, 892, 1971 |
| BanII | GRGCYC | 2 cut(s) | 164, 282 |
| Bbv12I | GWGCWC | 1 cut(s) | 282 |
| BccI | CCATC | 8 cut(s) | 27, 43, 285, 547, 1201, 1429, 1502, 1600 |
| BceAI | ACGGC | 3 cut(s) | 214, 1214, 1341 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 217, 251, 416, 450 |
| BciVI | GTATCC | 1 cut(s) | 1205 |
| BclI | TGATCA | 1 cut(s) | 1219 |
| BcnI | CCSGG | 1 cut(s) | 1014 |
| BcoDI | GTCTC | 1 cut(s) | 822 |
| BcuI | ACTAGT | 1 cut(s) | 1018 |
| BfaI | CTAG | 3 cut(s) | 444, 852, 1019 |
| BfmI | CTRYAG | 2 cut(s) | 1303, 1988 |
| BfoI | RGCGCY | 1 cut(s) | 506 |
| BfrI | CTTAAG | 1 cut(s) | 398 |
| BfuI | GTATCC | 1 cut(s) | 1205 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 962 |
| BglII | AGATCT | 1 cut(s) | 2176 |
| Bme1390I | CCNGG | 1 cut(s) | 1014 |
| Bme18I | GGWCC | 6 cut(s) | 294, 313, 779, 816, 946, 2206 |
| BmeT110I | CYCGRG | 2 cut(s) | 653, 1159 |
| BmgT120I | GGNCC | 7 cut(s) | 37, 294, 313, 779, 816, 946, 2206 |
| BmiI | GGNNCC | 7 cut(s) | 38, 93, 378, 894, 1973, 2207, 2208 |
| BmrFI | CCNGG | 1 cut(s) | 1014 |
| BmrI | ACTGGG | 2 cut(s) | 609, 1911 |
| BmsI | GCATC | 3 cut(s) | 261, 379, 800 |
| BmuI | ACTGGG | 2 cut(s) | 609, 1911 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1073 |
| Bpu14I | TTCGAA | 2 cut(s) | 576, 1501 |
| BpuEI | CTTGAG | 7 cut(s) | 368, 703, 1061, 1184, 1391, 1544, 2219 |
| BpuMI | CCSGG | 1 cut(s) | 1014 |
| BsaBI | GATNNNNATC | 1 cut(s) | 53 |
| BsaHI | GRCGYC | 1 cut(s) | 204 |
| BsaJI | CCNNGG | 4 cut(s) | 372, 883, 942, 1779 |
| BsaWI | WCCGGW | 2 cut(s) | 88, 315 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 83, 113, 540, 570 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 630, 721, 889, 1702, 2203 |
| Bse1I | ACTGG | 6 cut(s) | 604, 1561, 1568, 1917, 2061, 2106 |
| Bse3DI | GCAATG | 1 cut(s) | 1415 |
| Bse8I | GATNNNNATC | 1 cut(s) | 53 |
| BseDI | CCNNGG | 4 cut(s) | 372, 883, 942, 1779 |
| BseGI | GGATG | 6 cut(s) | 38, 394, 815, 847, 861, 1999 |
| BseJI | GATNNNNATC | 1 cut(s) | 53 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 630, 721, 889, 1702, 2203 |
| BseMI | GCAATG | 1 cut(s) | 1415 |
| BseMII | CTCAG | 5 cut(s) | 110, 236, 1551, 2100, 2202 |
| BseNI | ACTGG | 6 cut(s) | 604, 1561, 1568, 1917, 2061, 2106 |
| BseRI | GAGGAG | 4 cut(s) | 581, 584, 689, 815 |
| BseYI | CCCAGC | 1 cut(s) | 1151 |
| BsgI | GTGCAG | 3 cut(s) | 773, 1185, 2037 |
| Bsh1236I | CGCG | 1 cut(s) | 328 |
| Bsh1285I | CGRYCG | 1 cut(s) | 349 |
| BshFI | GGCC | 7 cut(s) | 39, 623, 680, 956, 1109, 1229, 1567 |
| BsiEI | CGRYCG | 1 cut(s) | 349 |
| BsiHKAI | GWGCWC | 1 cut(s) | 282 |
| BsiHKCI | CYCGRG | 2 cut(s) | 653, 1159 |
| BsiSI | CCGG | 5 cut(s) | 89, 316, 814, 1013, 2144 |
| BslFI | GGGAC | 5 cut(s) | 461, 617, 636, 1029, 2219 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 630, 721, 889, 1702, 2203 |
| BsmAI | GTCTC | 1 cut(s) | 822 |
| BsmBI | CGTCTC | 1 cut(s) | 822 |
| BsmFI | GGGAC | 5 cut(s) | 461, 617, 636, 1029, 2219 |
| BsmI | GAATGC | 2 cut(s) | 439, 1389 |
| BsnI | GGCC | 7 cut(s) | 39, 623, 680, 956, 1109, 1229, 1567 |
| BsoBI | CYCGRG | 2 cut(s) | 653, 1159 |
| Bsp119I | TTCGAA | 2 cut(s) | 576, 1501 |
| Bsp1286I | GDGCHC | 2 cut(s) | 164, 282 |
| BspACI | CCGC | 4 cut(s) | 349, 380, 671, 1985 |
| BspANI | GGCC | 7 cut(s) | 39, 623, 680, 956, 1109, 1229, 1567 |
| BspCNI | CTCAG | 5 cut(s) | 109, 237, 1550, 2101, 2203 |
| BspFNI | CGCG | 1 cut(s) | 328 |
| BspHI | TCATGA | 1 cut(s) | 267 |
| BspLI | GGNNCC | 7 cut(s) | 38, 93, 378, 894, 1973, 2207, 2208 |
| BspMAI | CTGCAG | 2 cut(s) | 1307, 1992 |
| BspQI | GCTCTTC | 3 cut(s) | 152, 1103, 1775 |
| BspT104I | TTCGAA | 2 cut(s) | 576, 1501 |
| BspTI | CTTAAG | 1 cut(s) | 398 |
| BsrBI | CCGCTC | 1 cut(s) | 382 |
| BsrDI | GCAATG | 1 cut(s) | 1415 |
| BsrI | ACTGG | 6 cut(s) | 604, 1561, 1568, 1917, 2061, 2106 |
| BssECI | CCNNGG | 4 cut(s) | 372, 883, 942, 1779 |
| BssNAI | GTATAC | 1 cut(s) | 2040 |
| BssNI | GRCGYC | 1 cut(s) | 204 |
| BssT1I | CCWWGG | 3 cut(s) | 372, 883, 942 |
| Bst1107I | GTATAC | 1 cut(s) | 2040 |
| Bst4CI | ACNGT | 5 cut(s) | 232, 1261, 1422, 1484, 2124 |
| Bst6I | CTCTTC | 7 cut(s) | 62, 152, 954, 1103, 1596, 1775, 2066 |
| BstACI | GRCGYC | 1 cut(s) | 204 |
| BstAFI | CTTAAG | 1 cut(s) | 398 |
| BstBI | TTCGAA | 2 cut(s) | 576, 1501 |
| BstC8I | GCNNGC | 3 cut(s) | 731, 1176, 1266 |
| BstDEI | CTNAG | 8 cut(s) | 96, 245, 1073, 1431, 1537, 1941, 2109, 2211 |
| BstF5I | GGATG | 6 cut(s) | 38, 394, 815, 847, 861, 1999 |
| BstFNI | CGCG | 1 cut(s) | 328 |
| BstH2I | RGCGCY | 1 cut(s) | 506 |
| BstHHI | GCGC | 5 cut(s) | 330, 505, 2034, 2133, 2169 |
| BstMAI | GTCTC | 1 cut(s) | 822 |
| BstMCI | CGRYCG | 1 cut(s) | 349 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 677, 962, 971, 1405, 1987 |
| BstNSI | RCATGY | 2 cut(s) | 148, 923 |
| BstSCI | CCNGG | 1 cut(s) | 1012 |
| BstSFI | CTRYAG | 2 cut(s) | 1303, 1988 |
| BstUI | CGCG | 1 cut(s) | 328 |
| BstX2I | RGATCY | 7 cut(s) | 121, 376, 892, 1285, 1878, 1971, 2176 |
| BstYI | RGATCY | 7 cut(s) | 121, 376, 892, 1285, 1878, 1971, 2176 |
| BstZ17I | GTATAC | 1 cut(s) | 2040 |
| BsuI | GTATCC | 1 cut(s) | 1205 |
| BsuRI | GGCC | 7 cut(s) | 39, 623, 680, 956, 1109, 1229, 1567 |
| BtsCI | GGATG | 6 cut(s) | 38, 394, 815, 847, 861, 1999 |
| BtsIMutI | CAGTG | 4 cut(s) | 1549, 1924, 2054, 2106 |
| Cac8I | GCNNGC | 3 cut(s) | 731, 1176, 1266 |
| CaiI | CAGNNNCTG | 1 cut(s) | 974 |
| CciI | TCATGA | 1 cut(s) | 267 |
| CfoI | GCGC | 5 cut(s) | 330, 505, 2034, 2133, 2169 |
| Cfr13I | GGNCC | 7 cut(s) | 37, 294, 313, 779, 816, 946, 2206 |
| CseI | GACGC | 2 cut(s) | 212, 2057 |
| Csp6I | GTAC | 6 cut(s) | 217, 1057, 1156, 1512, 1689, 1873 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 976, 1011 |
| CviQI | GTAC | 6 cut(s) | 217, 1057, 1156, 1512, 1689, 1873 |
| DdeI | CTNAG | 8 cut(s) | 96, 245, 1073, 1431, 1537, 1941, 2109, 2211 |
| DraIII | CACNNNGTG | 1 cut(s) | 2112 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 209 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 209 |
| EaeI | YGGCCR | 1 cut(s) | 1565 |
| Eam1104I | CTCTTC | 7 cut(s) | 62, 152, 954, 1103, 1596, 1775, 2066 |
| EarI | CTCTTC | 7 cut(s) | 62, 152, 954, 1103, 1596, 1775, 2066 |
| EciI | GGCGGA | 1 cut(s) | 686 |
| Ecl136II | GAGCTC | 1 cut(s) | 280 |
| Eco130I | CCWWGG | 3 cut(s) | 372, 883, 942 |
| Eco147I | AGGCCT | 3 cut(s) | 623, 680, 1109 |
| Eco24I | GRGCYC | 2 cut(s) | 164, 282 |
| Eco47I | GGWCC | 6 cut(s) | 294, 313, 779, 816, 946, 2206 |
| Eco47III | AGCGCT | 1 cut(s) | 504 |
| Eco53kI | GAGCTC | 1 cut(s) | 280 |
| Eco57I | CTGAAG | 2 cut(s) | 86, 1620 |
| Eco88I | CYCGRG | 2 cut(s) | 653, 1159 |
| EcoICRI | GAGCTC | 1 cut(s) | 280 |
| EcoO109I | RGGNCCY | 1 cut(s) | 2206 |
| EcoRI | GAATTC | 2 cut(s) | 72, 1298 |
| EcoT14I | CCWWGG | 3 cut(s) | 372, 883, 942 |
| EcoT38I | GRGCYC | 2 cut(s) | 164, 282 |
| ErhI | CCWWGG | 3 cut(s) | 372, 883, 942 |
| Esp3I | CGTCTC | 1 cut(s) | 822 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1227, 1259, 1482, 1514 |
| FaqI | GGGAC | 5 cut(s) | 461, 617, 636, 1029, 2219 |
| FauNDI | CATATG | 1 cut(s) | 42 |
| FbaI | TGATCA | 1 cut(s) | 1219 |
| FblI | GTMKAC | 3 cut(s) | 227, 2022, 2039 |
| FokI | GGATG | 6 cut(s) | 45, 401, 822, 854, 868, 2006 |
| FriOI | GRGCYC | 2 cut(s) | 164, 282 |
| FspBI | CTAG | 3 cut(s) | 444, 852, 1019 |
| GlaI | GCGC | 5 cut(s) | 329, 504, 2033, 2132, 2168 |
| GsaI | CCCAGC | 1 cut(s) | 1155 |
| HaeII | RGCGCY | 1 cut(s) | 506 |
| HaeIII | GGCC | 7 cut(s) | 39, 623, 680, 956, 1109, 1229, 1567 |
| HapII | CCGG | 5 cut(s) | 89, 316, 814, 1013, 2144 |
| HgaI | GACGC | 2 cut(s) | 212, 2057 |
| HhaI | GCGC | 5 cut(s) | 330, 505, 2034, 2133, 2169 |
| Hin1I | GRCGYC | 1 cut(s) | 204 |
| Hin6I | GCGC | 5 cut(s) | 328, 503, 2032, 2131, 2167 |
| HinP1I | GCGC | 5 cut(s) | 328, 503, 2032, 2131, 2167 |
| HincII | GTYRAC | 3 cut(s) | 228, 1743, 2023 |
| HindII | GTYRAC | 3 cut(s) | 228, 1743, 2023 |
| HindIII | AAGCTT | 2 cut(s) | 1520, 1664 |
| HinfI | GANTC | 7 cut(s) | 224, 449, 644, 803, 1810, 2074, 2215 |
| HpaII | CCGG | 5 cut(s) | 89, 316, 814, 1013, 2144 |
| HphI | GGTGA | 8 cut(s) | 49, 568, 1019, 1417, 1583, 1939, 2071, 2176 |
| Hpy166II | GTNNAC | 9 cut(s) | 228, 421, 782, 1425, 1743, 1840, 1947, 2023, 2040 |
| Hpy188I | TCNGA | 7 cut(s) | 156, 178, 287, 456, 1585, 1783, 1801 |
| Hpy8I | GTNNAC | 9 cut(s) | 228, 421, 782, 1425, 1743, 1840, 1947, 2023, 2040 |
| Hpy99I | CGWCG | 4 cut(s) | 206, 215, 232, 263 |
| HpyCH4III | ACNGT | 5 cut(s) | 232, 1261, 1422, 1484, 2124 |
| HpyCH4IV | ACGT | 5 cut(s) | 357, 417, 478, 516, 2135 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 677, 962, 971, 1405, 1987 |
| HpyF3I | CTNAG | 8 cut(s) | 96, 245, 1073, 1431, 1537, 1941, 2109, 2211 |
| HpySE526I | ACGT | 5 cut(s) | 357, 417, 478, 516, 2135 |
| Hsp92I | GRCGYC | 1 cut(s) | 204 |
| HspAI | GCGC | 5 cut(s) | 328, 503, 2032, 2131, 2167 |
| KflI | GGGWCCC | 1 cut(s) | 2206 |
| Ksp22I | TGATCA | 1 cut(s) | 1219 |
| LguI | GCTCTTC | 3 cut(s) | 152, 1103, 1775 |
| LmnI | GCTCC | 2 cut(s) | 91, 487 |
| LweI | GCATC | 3 cut(s) | 261, 379, 800 |
| MaeI | CTAG | 3 cut(s) | 444, 852, 1019 |
| MaeII | ACGT | 5 cut(s) | 357, 417, 478, 516, 2135 |
| MaeIII | GTNAC | 8 cut(s) | 922, 1255, 1447, 1559, 1919, 2008, 2104, 2189 |
| MbiI | CCGCTC | 1 cut(s) | 382 |
| MfeI | CAATTG | 1 cut(s) | 2043 |
| MflI | RGATCY | 7 cut(s) | 121, 376, 892, 1285, 1878, 1971, 2176 |
| MhlI | GDGCHC | 2 cut(s) | 164, 282 |
| MlsI | TGGCCA | 1 cut(s) | 1567 |
| MluCI | AATT | 9 cut(s) | 12, 72, 193, 559, 1169, 1298, 1787, 1814, 2043 |
| MluNI | TGGCCA | 1 cut(s) | 1567 |
| MlyI | GAGTC | 5 cut(s) | 233, 443, 812, 1804, 2083 |
| MmeI | TCCRAC | 6 cut(s) | 321, 520, 723, 870, 973, 1509 |
| Mox20I | TGGCCA | 1 cut(s) | 1567 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1484 |
| MscI | TGGCCA | 1 cut(s) | 1567 |
| MseI | TTAA | 5 cut(s) | 399, 408, 912, 1335, 1817 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1622, 1730, 1928 |
| Msp20I | TGGCCA | 1 cut(s) | 1567 |
| MspA1I | CMGCKG | 3 cut(s) | 787, 974, 1987 |
| MspCI | CTTAAG | 1 cut(s) | 398 |
| MspI | CCGG | 5 cut(s) | 89, 316, 814, 1013, 2144 |
| MspR9I | CCNGG | 1 cut(s) | 1014 |
| MunI | CAATTG | 1 cut(s) | 2043 |
| Mva1269I | GAATGC | 2 cut(s) | 439, 1389 |
| MvnI | CGCG | 1 cut(s) | 328 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 677, 962, 971, 1405, 1987 |
| NciI | CCSGG | 1 cut(s) | 1014 |
| NdeI | CATATG | 1 cut(s) | 42 |
| NlaIV | GGNNCC | 7 cut(s) | 38, 93, 378, 894, 1973, 2207, 2208 |
| NmuCI | GTSAC | 5 cut(s) | 1447, 1919, 2008, 2104, 2189 |
| NspI | RCATGY | 2 cut(s) | 148, 923 |
| NspV | TTCGAA | 2 cut(s) | 576, 1501 |
| PaeR7I | CTCGAG | 1 cut(s) | 1159 |
| PagI | TCATGA | 1 cut(s) | 267 |
| PceI | AGGCCT | 3 cut(s) | 623, 680, 1109 |
| PciI | ACATGT | 1 cut(s) | 144 |
| PciSI | GCTCTTC | 3 cut(s) | 152, 1103, 1775 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 210, 219, 423 |
| PctI | GAATGC | 2 cut(s) | 439, 1389 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1484 |
| PfeI | GAWTC | 2 cut(s) | 644, 2215 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 889 |
| PleI | GAGTC | 5 cut(s) | 232, 443, 811, 1804, 2082 |
| PpsI | GAGTC | 5 cut(s) | 232, 443, 811, 1804, 2082 |
| PpuMI | RGGWCCY | 1 cut(s) | 2206 |
| PscI | ACATGT | 1 cut(s) | 144 |
| PshBI | ATTAAT | 1 cut(s) | 1335 |
| Psp124BI | GAGCTC | 1 cut(s) | 282 |
| Psp5II | RGGWCCY | 1 cut(s) | 2206 |
| PspFI | CCCAGC | 1 cut(s) | 1151 |
| PspN4I | GGNNCC | 7 cut(s) | 38, 93, 378, 894, 1973, 2207, 2208 |
| PspPI | GGNCC | 7 cut(s) | 37, 294, 313, 779, 816, 946, 2206 |
| PspPPI | RGGWCCY | 1 cut(s) | 2206 |
| PspXI | VCTCGAGB | 1 cut(s) | 1159 |
| PstI | CTGCAG | 2 cut(s) | 1307, 1992 |
| PstNI | CAGNNNCTG | 1 cut(s) | 974 |
| PsuI | RGATCY | 7 cut(s) | 121, 376, 892, 1285, 1878, 1971, 2176 |
| PvuII | CAGCTG | 2 cut(s) | 787, 974 |
| RsaI | GTAC | 6 cut(s) | 218, 1058, 1157, 1513, 1690, 1874 |
| RsaNI | GTAC | 6 cut(s) | 217, 1057, 1156, 1512, 1689, 1873 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1622, 1730, 1928 |
| SacI | GAGCTC | 1 cut(s) | 282 |
| SalI | GTCGAC | 2 cut(s) | 226, 2021 |
| SapI | GCTCTTC | 3 cut(s) | 152, 1103, 1775 |
| SaqAI | TTAA | 5 cut(s) | 399, 408, 912, 1335, 1817 |
| Sau96I | GGNCC | 7 cut(s) | 37, 294, 313, 779, 816, 946, 2206 |
| SchI | GAGTC | 5 cut(s) | 233, 443, 812, 1804, 2083 |
| ScrFI | CCNGG | 1 cut(s) | 1014 |
| SduI | GDGCHC | 2 cut(s) | 164, 282 |
| SfaNI | GCATC | 3 cut(s) | 261, 379, 800 |
| SfcI | CTRYAG | 2 cut(s) | 1303, 1988 |
| Sfr274I | CTCGAG | 1 cut(s) | 1159 |
| SfuI | TTCGAA | 2 cut(s) | 576, 1501 |
| SinI | GGWCC | 6 cut(s) | 294, 313, 779, 816, 946, 2206 |
| SlaI | CTCGAG | 1 cut(s) | 1159 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1622, 1730, 1928 |
| SmlI | CTYRAG | 9 cut(s) | 383, 398, 682, 1040, 1159, 1199, 1370, 1523, 2198 |
| SmoI | CTYRAG | 9 cut(s) | 383, 398, 682, 1040, 1159, 1199, 1370, 1523, 2198 |
| SpeI | ACTAGT | 1 cut(s) | 1018 |
| Sse9I | AATT | 9 cut(s) | 12, 72, 193, 559, 1169, 1298, 1787, 1814, 2043 |
| SseBI | AGGCCT | 3 cut(s) | 623, 680, 1109 |
| SsiI | CCGC | 4 cut(s) | 349, 380, 671, 1985 |
| SspI | AATATT | 2 cut(s) | 1637, 2085 |
| SspMI | CTAG | 3 cut(s) | 444, 852, 1019 |
| SstI | GAGCTC | 1 cut(s) | 282 |
| StuI | AGGCCT | 3 cut(s) | 623, 680, 1109 |
| StyD4I | CCNGG | 1 cut(s) | 1012 |
| StyI | CCWWGG | 3 cut(s) | 372, 883, 942 |
| TaaI | ACNGT | 5 cut(s) | 232, 1261, 1422, 1484, 2124 |
| TaiI | ACGT | 5 cut(s) | 360, 420, 481, 519, 2138 |
| TasI | AATT | 9 cut(s) | 12, 72, 193, 559, 1169, 1298, 1787, 1814, 2043 |
| TatI | WGTACW | 3 cut(s) | 216, 1056, 1872 |
| TfiI | GAWTC | 2 cut(s) | 644, 2215 |
| Tru1I | TTAA | 5 cut(s) | 399, 408, 912, 1335, 1817 |
| Tru9I | TTAA | 5 cut(s) | 399, 408, 912, 1335, 1817 |
| TscAI | CASTG | 4 cut(s) | 1549, 1924, 2061, 2113 |
| TseFI | GTSAC | 5 cut(s) | 1447, 1919, 2008, 2104, 2189 |
| Tsp45I | GTSAC | 5 cut(s) | 1447, 1919, 2008, 2104, 2189 |
| TspGWI | ACGGA | 1 cut(s) | 2027 |
| TspRI | CASTG | 4 cut(s) | 1549, 1924, 2061, 2113 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 889 |
| Vha464I | CTTAAG | 1 cut(s) | 398 |
| VpaK11BI | GGWCC | 6 cut(s) | 294, 313, 779, 816, 946, 2206 |
| VspI | ATTAAT | 1 cut(s) | 1335 |
| XapI | RAATTY | 2 cut(s) | 72, 1298 |
| XceI | RCATGY | 2 cut(s) | 148, 923 |
| XhoI | CTCGAG | 1 cut(s) | 1159 |
| XmiI | GTMKAC | 3 cut(s) | 227, 2022, 2039 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1484 |
| XspI | CTAG | 3 cut(s) | 444, 852, 1019 |