Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1339 |
| AbsI | CCTCGAGG | 1 cut(s) | 891 |
| Acc36I | ACCTGC | 1 cut(s) | 1015 |
| AccB1I | GGYRCC | 1 cut(s) | 819 |
| AccBSI | CCGCTC | 1 cut(s) | 16 |
| AccI | GTMKAC | 2 cut(s) | 429, 2013 |
| AciI | CCGC | 3 cut(s) | 16, 170, 612 |
| AclWI | GGATC | 7 cut(s) | 42, 71, 650, 896, 909, 1867, 1967 |
| AcoI | YGGCCR | 1 cut(s) | 1559 |
| AcsI | RAATTY | 5 cut(s) | 1310, 1628, 1703, 1813, 1925 |
| AcuI | CTGAAG | 2 cut(s) | 960, 987 |
| AcyI | GRCGYC | 1 cut(s) | 1336 |
| AfaI | GTAC | 6 cut(s) | 206, 227, 1064, 1684, 1868, 2225 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1882 |
| AflII | CTTAAG | 1 cut(s) | 1205 |
| AjnI | CCWGG | 1 cut(s) | 1516 |
| Alw21I | GWGCWC | 3 cut(s) | 161, 514, 813 |
| Alw26I | GTCTC | 1 cut(s) | 2175 |
| Alw44I | GTGCAC | 1 cut(s) | 157 |
| AlwI | GGATC | 7 cut(s) | 42, 71, 650, 896, 909, 1867, 1967 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 799 |
| Ama87I | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| AoxI | GGCC | 3 cut(s) | 1125, 1559, 2229 |
| ApaLI | GTGCAC | 1 cut(s) | 157 |
| ApeKI | GCWGC | 7 cut(s) | 590, 925, 974, 1273, 1748, 1751, 2113 |
| ApoI | RAATTY | 5 cut(s) | 1310, 1628, 1703, 1813, 1925 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 13, 45, 566, 598 |
| AsuHPI | GGTGA | 2 cut(s) | 304, 1411 |
| AsuII | TTCGAA | 1 cut(s) | 219 |
| AvaI | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| BaeGI | GKGCMC | 1 cut(s) | 161 |
| BalI | TGGCCA | 1 cut(s) | 1561 |
| BamHI | GGATCC | 1 cut(s) | 901 |
| BanI | GGYRCC | 1 cut(s) | 819 |
| BanII | GRGCYC | 1 cut(s) | 514 |
| BbsI | GAAGAC | 3 cut(s) | 26, 1331, 1649 |
| Bbv12I | GWGCWC | 3 cut(s) | 161, 514, 813 |
| BbvI | GCAGC | 7 cut(s) | 602, 937, 986, 1260, 1735, 1763, 2125 |
| BccI | CCATC | 5 cut(s) | 36, 256, 1423, 1432, 1910 |
| BceAI | ACGGC | 1 cut(s) | 2052 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2102, 2136 |
| BciT130I | CCWGG | 1 cut(s) | 1518 |
| BclI | TGATCA | 1 cut(s) | 478 |
| BcoDI | GTCTC | 1 cut(s) | 2175 |
| BfaI | CTAG | 3 cut(s) | 86, 1067, 1167 |
| BfmI | CTRYAG | 3 cut(s) | 1016, 1315, 1749 |
| BfrI | CTTAAG | 1 cut(s) | 1205 |
| BfuAI | ACCTGC | 1 cut(s) | 1015 |
| BglII | AGATCT | 2 cut(s) | 421, 1134 |
| BisI | GCNGC | 9 cut(s) | 170, 591, 612, 926, 975, 1274, 1749, 1752, 2114 |
| BlpI | GCTNAGC | 1 cut(s) | 1596 |
| BlsI | GCNGC | 9 cut(s) | 171, 592, 613, 927, 976, 1275, 1750, 1753, 2115 |
| Bme1390I | CCNGG | 1 cut(s) | 1518 |
| BmeT110I | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| BmiI | GGNNCC | 3 cut(s) | 821, 903, 1887 |
| BmrFI | CCNGG | 1 cut(s) | 1518 |
| BmrI | ACTGGG | 1 cut(s) | 1688 |
| BmsI | GCATC | 5 cut(s) | 120, 448, 585, 961, 2029 |
| BmuI | ACTGGG | 1 cut(s) | 1688 |
| BpiI | GAAGAC | 3 cut(s) | 26, 1331, 1649 |
| BpmI | CTGGAG | 2 cut(s) | 715, 821 |
| Bpu10I | CCTNAGC | 1 cut(s) | 744 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1596 |
| Bpu14I | TTCGAA | 1 cut(s) | 219 |
| BpuEI | CTTGAG | 2 cut(s) | 773, 1103 |
| Bsa29I | ATCGAT | 1 cut(s) | 1780 |
| BsaAI | YACGTR | 1 cut(s) | 1665 |
| BsaBI | GATNNNNATC | 1 cut(s) | 33 |
| BsaHI | GRCGYC | 1 cut(s) | 1336 |
| BsaJI | CCNNGG | 3 cut(s) | 117, 237, 1517 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 713, 743 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1882 |
| Bse1I | ACTGG | 4 cut(s) | 732, 905, 1555, 1683 |
| Bse8I | GATNNNNATC | 1 cut(s) | 33 |
| BseBI | CCWGG | 1 cut(s) | 1518 |
| BseCI | ATCGAT | 1 cut(s) | 1780 |
| BseDI | CCNNGG | 3 cut(s) | 117, 237, 1517 |
| BseGI | GGATG | 6 cut(s) | 47, 856, 1009, 1443, 1674, 1990 |
| BseJI | GATNNNNATC | 1 cut(s) | 33 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1882 |
| BseMII | CTCAG | 4 cut(s) | 119, 806, 1587, 1788 |
| BseNI | ACTGG | 4 cut(s) | 732, 905, 1555, 1683 |
| BseRI | GAGGAG | 1 cut(s) | 1969 |
| BseSI | GKGCMC | 1 cut(s) | 161 |
| BseXI | GCAGC | 7 cut(s) | 602, 937, 986, 1260, 1735, 1763, 2125 |
| BsgI | GTGCAG | 1 cut(s) | 1191 |
| BshFI | GGCC | 3 cut(s) | 1127, 1561, 2231 |
| BshNI | GGYRCC | 1 cut(s) | 819 |
| BshVI | ATCGAT | 1 cut(s) | 1780 |
| BsiHKAI | GWGCWC | 3 cut(s) | 161, 514, 813 |
| BsiHKCI | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| BsiSI | CCGG | 1 cut(s) | 2135 |
| BslFI | GGGAC | 1 cut(s) | 2094 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1882 |
| BsmAI | GTCTC | 1 cut(s) | 2175 |
| BsmFI | GGGAC | 1 cut(s) | 2094 |
| BsnI | GGCC | 3 cut(s) | 1127, 1561, 2231 |
| BsoBI | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| Bsp119I | TTCGAA | 1 cut(s) | 219 |
| Bsp1286I | GDGCHC | 3 cut(s) | 161, 514, 813 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1596 |
| Bsp19I | CCATGG | 1 cut(s) | 237 |
| BspACI | CCGC | 3 cut(s) | 16, 170, 612 |
| BspANI | GGCC | 3 cut(s) | 1127, 1561, 2231 |
| BspCNI | CTCAG | 4 cut(s) | 118, 805, 1588, 1787 |
| BspDI | ATCGAT | 1 cut(s) | 1780 |
| BspHI | TCATGA | 3 cut(s) | 1054, 1800, 2058 |
| BspLI | GGNNCC | 3 cut(s) | 821, 903, 1887 |
| BspMAI | CTGCAG | 2 cut(s) | 1020, 1753 |
| BspMI | ACCTGC | 1 cut(s) | 1015 |
| BspPI | GGATC | 7 cut(s) | 42, 71, 650, 896, 909, 1867, 1967 |
| BspQI | GCTCTTC | 1 cut(s) | 2057 |
| BspT104I | TTCGAA | 1 cut(s) | 219 |
| BspT107I | GGYRCC | 1 cut(s) | 819 |
| BspTI | CTTAAG | 1 cut(s) | 1205 |
| BsrBI | CCGCTC | 1 cut(s) | 16 |
| BsrI | ACTGG | 4 cut(s) | 732, 905, 1555, 1683 |
| BssECI | CCNNGG | 3 cut(s) | 117, 237, 1517 |
| BssNI | GRCGYC | 1 cut(s) | 1336 |
| BssT1I | CCWWGG | 1 cut(s) | 237 |
| Bst2UI | CCWGG | 1 cut(s) | 1518 |
| Bst4CI | ACNGT | 3 cut(s) | 1534, 1540, 2028 |
| Bst6I | CTCTTC | 3 cut(s) | 216, 1977, 2057 |
| BstACI | GRCGYC | 1 cut(s) | 1336 |
| BstAFI | CTTAAG | 1 cut(s) | 1205 |
| BstBAI | YACGTR | 1 cut(s) | 1665 |
| BstBI | TTCGAA | 1 cut(s) | 219 |
| BstC8I | GCNNGC | 4 cut(s) | 979, 1278, 1329, 1509 |
| BstDEI | CTNAG | 9 cut(s) | 105, 281, 646, 744, 792, 882, 952, 1596, 1774 |
| BstDSI | CCRYGG | 1 cut(s) | 237 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1880 |
| BstF5I | GGATG | 6 cut(s) | 47, 856, 1009, 1443, 1674, 1990 |
| BstMAI | GTCTC | 1 cut(s) | 2175 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 253, 262, 286, 971, 2039 |
| BstNI | CCWGG | 1 cut(s) | 1518 |
| BstNSI | RCATGY | 2 cut(s) | 932, 2128 |
| BstSCI | CCNGG | 1 cut(s) | 1516 |
| BstSFI | CTRYAG | 3 cut(s) | 1016, 1315, 1749 |
| BstSLI | GKGCMC | 1 cut(s) | 161 |
| BstV1I | GCAGC | 7 cut(s) | 602, 937, 986, 1260, 1735, 1763, 2125 |
| BstV2I | GAAGAC | 3 cut(s) | 26, 1331, 1649 |
| BstX2I | RGATCY | 5 cut(s) | 421, 642, 901, 1134, 1872 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 31 |
| BstYI | RGATCY | 5 cut(s) | 421, 642, 901, 1134, 1872 |
| Bsu15I | ATCGAT | 1 cut(s) | 1780 |
| BsuRI | GGCC | 3 cut(s) | 1127, 1561, 2231 |
| BsuTUI | ATCGAT | 1 cut(s) | 1780 |
| BtgI | CCRYGG | 1 cut(s) | 237 |
| BtgZI | GCGATG | 1 cut(s) | 608 |
| BtsCI | GGATG | 6 cut(s) | 47, 856, 1009, 1443, 1674, 1990 |
| BtsI | GCAGTG | 2 cut(s) | 606, 827 |
| BtsIMutI | CAGTG | 4 cut(s) | 606, 827, 1676, 1702 |
| BveI | ACCTGC | 1 cut(s) | 1015 |
| Cac8I | GCNNGC | 4 cut(s) | 979, 1278, 1329, 1509 |
| CaiI | CAGNNNCTG | 1 cut(s) | 799 |
| CciI | TCATGA | 3 cut(s) | 1054, 1800, 2058 |
| ClaI | ATCGAT | 1 cut(s) | 1780 |
| Csp6I | GTAC | 6 cut(s) | 205, 226, 1063, 1683, 1867, 2224 |
| CviQI | GTAC | 6 cut(s) | 205, 226, 1063, 1683, 1867, 2224 |
| DdeI | CTNAG | 9 cut(s) | 105, 281, 646, 744, 792, 882, 952, 1596, 1774 |
| EaeI | YGGCCR | 1 cut(s) | 1559 |
| Eam1104I | CTCTTC | 3 cut(s) | 216, 1977, 2057 |
| EarI | CTCTTC | 3 cut(s) | 216, 1977, 2057 |
| Ecl136II | GAGCTC | 1 cut(s) | 512 |
| Eco130I | CCWWGG | 1 cut(s) | 237 |
| Eco147I | AGGCCT | 1 cut(s) | 1127 |
| Eco24I | GRGCYC | 1 cut(s) | 514 |
| Eco53kI | GAGCTC | 1 cut(s) | 512 |
| Eco57I | CTGAAG | 2 cut(s) | 960, 987 |
| Eco88I | CYCGRG | 3 cut(s) | 98, 891, 1256 |
| EcoICRI | GAGCTC | 1 cut(s) | 512 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1880 |
| EcoRI | GAATTC | 2 cut(s) | 1310, 1628 |
| EcoRII | CCWGG | 1 cut(s) | 1516 |
| EcoT14I | CCWWGG | 1 cut(s) | 237 |
| EcoT38I | GRGCYC | 1 cut(s) | 514 |
| ErhI | CCWWGG | 1 cut(s) | 237 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 390, 422, 1112, 1144 |
| FaqI | GGGAC | 1 cut(s) | 2094 |
| FauNDI | CATATG | 1 cut(s) | 1072 |
| FbaI | TGATCA | 1 cut(s) | 478 |
| FblI | GTMKAC | 2 cut(s) | 429, 2013 |
| Fnu4HI | GCNGC | 9 cut(s) | 170, 591, 612, 926, 975, 1274, 1749, 1752, 2114 |
| FokI | GGATG | 6 cut(s) | 54, 863, 1016, 1450, 1681, 1997 |
| FriOI | GRGCYC | 1 cut(s) | 514 |
| Fsp4HI | GCNGC | 9 cut(s) | 170, 591, 612, 926, 975, 1274, 1749, 1752, 2114 |
| FspBI | CTAG | 3 cut(s) | 86, 1067, 1167 |
| GluI | GCNGC | 9 cut(s) | 170, 591, 612, 926, 975, 1274, 1749, 1752, 2114 |
| GsuI | CTGGAG | 2 cut(s) | 715, 821 |
| HaeIII | GGCC | 3 cut(s) | 1127, 1561, 2231 |
| HapII | CCGG | 1 cut(s) | 2135 |
| Hin1I | GRCGYC | 1 cut(s) | 1336 |
| HincII | GTYRAC | 1 cut(s) | 1545 |
| HindII | GTYRAC | 1 cut(s) | 1545 |
| HindIII | AAGCTT | 3 cut(s) | 630, 1325, 1658 |
| HinfI | GANTC | 4 cut(s) | 212, 653, 1804, 1966 |
| HpaII | CCGG | 1 cut(s) | 2135 |
| HphI | GGTGA | 2 cut(s) | 304, 1411 |
| Hpy166II | GTNNAC | 6 cut(s) | 159, 430, 1419, 1545, 1941, 2014 |
| Hpy188I | TCNGA | 6 cut(s) | 118, 375, 474, 627, 1777, 1795 |
| Hpy8I | GTNNAC | 6 cut(s) | 159, 430, 1419, 1545, 1941, 2014 |
| Hpy99I | CGWCG | 1 cut(s) | 1338 |
| HpyAV | CCTTC | 7 cut(s) | 52, 83, 485, 935, 982, 1255, 1598 |
| HpyCH4III | ACNGT | 3 cut(s) | 1534, 1540, 2028 |
| HpyCH4IV | ACGT | 3 cut(s) | 1336, 1454, 1664 |
| HpyCH4V | TGCA | 9 cut(s) | 55, 159, 788, 827, 974, 1018, 1143, 1172, 1751 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 253, 262, 286, 971, 2039 |
| HpyF3I | CTNAG | 9 cut(s) | 105, 281, 646, 744, 792, 882, 952, 1596, 1774 |
| HpySE526I | ACGT | 3 cut(s) | 1336, 1454, 1664 |
| Hsp92I | GRCGYC | 1 cut(s) | 1336 |
| Ksp22I | TGATCA | 1 cut(s) | 478 |
| LguI | GCTCTTC | 1 cut(s) | 2057 |
| LmnI | GCTCC | 5 cut(s) | 119, 1021, 1848, 1885, 2110 |
| Lsp1109I | GCAGC | 7 cut(s) | 602, 937, 986, 1260, 1735, 1763, 2125 |
| LweI | GCATC | 5 cut(s) | 120, 448, 585, 961, 2029 |
| MaeI | CTAG | 3 cut(s) | 86, 1067, 1167 |
| MaeII | ACGT | 3 cut(s) | 1336, 1454, 1664 |
| MaeIII | GTNAC | 9 cut(s) | 378, 931, 1232, 1289, 1441, 1534, 1688, 1732, 1787 |
| MbiI | CCGCTC | 1 cut(s) | 16 |
| MfeI | CAATTG | 1 cut(s) | 637 |
| MflI | RGATCY | 5 cut(s) | 421, 642, 901, 1134, 1872 |
| MhlI | GDGCHC | 3 cut(s) | 161, 514, 813 |
| MlsI | TGGCCA | 1 cut(s) | 1561 |
| MluNI | TGGCCA | 1 cut(s) | 1561 |
| MlyI | GAGTC | 2 cut(s) | 221, 1798 |
| MmeI | TCCRAC | 1 cut(s) | 382 |
| Mox20I | TGGCCA | 1 cut(s) | 1561 |
| MscI | TGGCCA | 1 cut(s) | 1561 |
| Msp20I | TGGCCA | 1 cut(s) | 1561 |
| MspA1I | CMGCKG | 1 cut(s) | 1595 |
| MspCI | CTTAAG | 1 cut(s) | 1205 |
| MspI | CCGG | 1 cut(s) | 2135 |
| MspR9I | CCNGG | 1 cut(s) | 1518 |
| MunI | CAATTG | 1 cut(s) | 637 |
| MvaI | CCWGG | 1 cut(s) | 1518 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 253, 262, 286, 971, 2039 |
| NcoI | CCATGG | 1 cut(s) | 237 |
| NdeI | CATATG | 1 cut(s) | 1072 |
| NlaIV | GGNNCC | 3 cut(s) | 821, 903, 1887 |
| NmuCI | GTSAC | 4 cut(s) | 378, 1232, 1289, 1732 |
| NspI | RCATGY | 2 cut(s) | 932, 2128 |
| NspV | TTCGAA | 1 cut(s) | 219 |
| PaeR7I | CTCGAG | 2 cut(s) | 891, 1256 |
| PagI | TCATGA | 3 cut(s) | 1054, 1800, 2058 |
| PceI | AGGCCT | 1 cut(s) | 1127 |
| PciSI | GCTCTTC | 1 cut(s) | 2057 |
| PfeI | GAWTC | 2 cut(s) | 653, 1966 |
| PkrI | GCNGC | 9 cut(s) | 171, 592, 613, 927, 976, 1275, 1750, 1753, 2115 |
| PleI | GAGTC | 2 cut(s) | 220, 1798 |
| PpsI | GAGTC | 2 cut(s) | 220, 1798 |
| Ppu21I | YACGTR | 1 cut(s) | 1665 |
| Psp124BI | GAGCTC | 1 cut(s) | 514 |
| Psp6I | CCWGG | 1 cut(s) | 1516 |
| PspGI | CCWGG | 1 cut(s) | 1516 |
| PspN4I | GGNNCC | 3 cut(s) | 821, 903, 1887 |
| PspXI | VCTCGAGB | 1 cut(s) | 891 |
| PstI | CTGCAG | 2 cut(s) | 1020, 1753 |
| PstNI | CAGNNNCTG | 1 cut(s) | 799 |
| PsuI | RGATCY | 5 cut(s) | 421, 642, 901, 1134, 1872 |
| PvuII | CAGCTG | 1 cut(s) | 1595 |
| RsaI | GTAC | 6 cut(s) | 206, 227, 1064, 1684, 1868, 2225 |
| RsaNI | GTAC | 6 cut(s) | 205, 226, 1063, 1683, 1867, 2224 |
| SacI | GAGCTC | 1 cut(s) | 514 |
| SapI | GCTCTTC | 1 cut(s) | 2057 |
| SatI | GCNGC | 9 cut(s) | 170, 591, 612, 926, 975, 1274, 1749, 1752, 2114 |
| SchI | GAGTC | 2 cut(s) | 221, 1798 |
| ScrFI | CCNGG | 1 cut(s) | 1518 |
| SduI | GDGCHC | 3 cut(s) | 161, 514, 813 |
| SfaNI | GCATC | 5 cut(s) | 120, 448, 585, 961, 2029 |
| SfcI | CTRYAG | 3 cut(s) | 1016, 1315, 1749 |
| Sfr274I | CTCGAG | 2 cut(s) | 891, 1256 |
| SfuI | TTCGAA | 1 cut(s) | 219 |
| SlaI | CTCGAG | 2 cut(s) | 891, 1256 |
| SmlI | CTYRAG | 5 cut(s) | 752, 891, 1118, 1205, 1256 |
| SmoI | CTYRAG | 5 cut(s) | 752, 891, 1118, 1205, 1256 |
| SseBI | AGGCCT | 1 cut(s) | 1127 |
| SsiI | CCGC | 3 cut(s) | 16, 170, 612 |
| SspMI | CTAG | 3 cut(s) | 86, 1067, 1167 |
| SstI | GAGCTC | 1 cut(s) | 514 |
| StuI | AGGCCT | 1 cut(s) | 1127 |
| StyD4I | CCNGG | 1 cut(s) | 1516 |
| StyI | CCWWGG | 1 cut(s) | 237 |
| TaaI | ACNGT | 3 cut(s) | 1534, 1540, 2028 |
| TaiI | ACGT | 3 cut(s) | 1339, 1457, 1667 |
| TaqI | TCGA | 9 cut(s) | 219, 418, 835, 892, 1257, 1333, 1780, 1934, 2004 |
| TatI | WGTACW | 3 cut(s) | 204, 225, 1866 |
| TauI | GCSGC | 2 cut(s) | 172, 614 |
| TfiI | GAWTC | 2 cut(s) | 653, 1966 |
| TscAI | CASTG | 4 cut(s) | 613, 834, 1683, 1702 |
| TseFI | GTSAC | 4 cut(s) | 378, 1232, 1289, 1732 |
| TseI | GCWGC | 7 cut(s) | 590, 925, 974, 1273, 1748, 1751, 2113 |
| Tsp45I | GTSAC | 4 cut(s) | 378, 1232, 1289, 1732 |
| TspDTI | ATGAA | 5 cut(s) | 23, 265, 411, 1043, 2075 |
| TspRI | CASTG | 4 cut(s) | 613, 834, 1683, 1702 |
| Vha464I | CTTAAG | 1 cut(s) | 1205 |
| VneI | GTGCAC | 1 cut(s) | 157 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1880 |
| XapI | RAATTY | 5 cut(s) | 1310, 1628, 1703, 1813, 1925 |
| XbaI | TCTAGA | 1 cut(s) | 85 |
| XceI | RCATGY | 2 cut(s) | 932, 2128 |
| XhoI | CTCGAG | 2 cut(s) | 891, 1256 |
| XmiI | GTMKAC | 2 cut(s) | 429, 2013 |
| XspI | CTAG | 3 cut(s) | 86, 1067, 1167 |
| ZraI | GACGTC | 1 cut(s) | 1337 |